Академический Документы
Профессиональный Документы
Культура Документы
QUESTION 1
Gas chromatography (GC), liquid chromatography (LC), and supercritical fluid chromatography
(SFC) are three types of separation methods with different mobile phases.
ii) the types of sample that are suitable for each of the above separation method.
(6 marks)
b) For each of the above separation methods, discuss one main parameter (exclude flow
rate) that can be manipulated to improve the separation of analytes.
(6 marks)
QUESTION 2
Too much sample vented out, small amount of sample flow into the column (1), hence
small peak (1) and not reproducible (1).
ii) Too high split ratio was set for a split mode injection of a split/splitless injector.
(3 marks)
ii) Would increasing the isothermal column temperature to 250C improve the
separation? If not, suggest an approach to improve the separation.
Khonghy/CHM510/TUTORIAL 2 5
UiTM/FSG/AS202/CHM510
(6 marks)
c) In the analysis of phenols using gas chromatography (GC), discuss the followings :
ii) Mass spectrometry was chosen as the detector out of other common GC detectors.
(3 marks)
QUESTION 3
The analysis of polyaromatic hydrocarbons was carried out with the following GC conditions:
column, poly(phenylmethyl)siloxane (5% phenyl); injection, split 1:20, detector, mass
spectrometry; column temperature, 100C for 2 min then programmed to 350C at 10C/min.
The chromatogram is shown below.
Khonghy/CHM510/TUTORIAL 2 6
UiTM/FSG/AS202/CHM510
(3 marks)
ii) Is it possible to decrease the rentention time of naphthalene by increasing the initial
column temperature to 200C without affecting the separation of other compounds?
Explain.
(4 marks)
iii) Suggest another possible detector for this analysis? Verify your choice.
(3 marks)
Khonghy/CHM510/TUTORIAL 2 7
UiTM/FSG/AS202/CHM510
QUESTION 4
Figure 1
ii) Figure 2 shows a typical chromatogram observed for the separation of polar
analytes using PDMS column. Explain the cause of the observed peak tailing.
Suggest an approach to avoid this problem.
(3 marks)
Figure 2
d) The analysis of drug in urine was analyzed using a gas chromatography with
split/splitless injector. The drug was extracted using solid phase extraction and elution
Khonghy/CHM510/TUTORIAL 2 8
UiTM/FSG/AS202/CHM510
was achieved using methanol. Suggest a suitable injection mode for this analysis.
Explain.
(3 marks)
QUESTION 5
Explain why a split injection mode is not suitable for this analysis. Suggest an alternative
approach for sample injection in order to improve the separation of chlorphrifos.
QUESTION 6
Khonghy/CHM510/TUTORIAL 2 9
UiTM/FSG/AS202/CHM510
iii) Suggest a suitable carrier gas for the analysis. Verify your answer.
(3 marks)
Khonghy/CHM510/TUTORIAL 2
10