EXPERIMENT RESULT

Nu. Procedure Observationt Result Reaction Conclusion

1. Determine Peroxide Number
5 grams of sample (oil/lipid)
Poured into erlenmeyer
Added 30 mL acetic
chloroform solution
Shaken until diluted perfectly
Added 0,5 mL saturated solution
of KI
Waited until 20 minutes while
shaken
Added 30 mL of aquadest
Titrated with Na2S2O3
Before
- Aquadest : colorless
- Palm Oil : yellow solution
- Acetic acid chloroform :
acid- colorless
- Saturated KI solution :
colorless
- Na2S2O3 solution 0,1N :
colorless
- Starch solution 1% : colorless 2S2O32- S4O62- + 2e-
After
- Palm oil + acetic acid
CH3(CH2)7CH=CH(CH2)7COOH The peroxide
+3/2O2CH3(CH2)7CHCH(CH2)2COOH number is
+ H2O2 15,9792
meq/kg
H2O2 +2KI I2 + 2KOH
I2 + Na2S2O3 2NaI
I2 + 2e- 2I-
I2 + 2S2O32- S4O62-

Yellow (-) color solution chloroform : yellow (+++)

- Palm oil + acetic acid
chloroform + KI : yellow
(++)
Nu. Procedure Observationt Result Raction Conclusion
- Palm oil + acetic acid
chloroform + KI + aquadest :
2 layer, yellow (++) on
bottom, turbid colorless on
Yellow (-) color solution
top
Added 0,5 mL of starch solution 1% - Titrated with Na2S2O3 :
Titrated with Na2S2O3 0,1 N until yellow (+) on bottom, turbid
clear colorless on top
Calculated the volume of Na2S2O3 - (+) starch solution 1% +
0,1 N Titrated with Na2S2O3 : form
Peroxide Number 2 layer, pale yellow in bottom
colorless on top
Nu. Procedure Observationt Result Reaction Conclusion
Blanco for Peroxide Number Before

5 grams of diluted water - Aquadest : colorless

- Palm Oil : yellow solution
Poured into erlenmeyer
- Acetic acid chloroform :
Added 30 mL acetic acid-chloroform
colorless
solution
- Saturated KI solution :
Shaken until diluted perfectly
colorless
Added 0,5 mL saturated solution of KI
- Na2S2O3 solution 0,1N :
Waited until 20 minutes while shaken
colorless
Added 30 mL of aquadest - Starch solution 1% : colorless
Titrated with Na2S2O3 0,1 N
Yellow (-) color solution After
Added 0,5 mL of starch solution - Aquadest + acetic acid

Titrated with Na2S2O3 0,1 N until clear chloroform : colorless

Calculated the volume of Na2S2O3 - Aquadest + acetic acid

chloroform + KI : colorless
Peroxide Number
- After leaved 20 minutes :
yellow solution (+)
- Aquadest + acetic acid
chloroform + saturated KI +
aquadest : colorless
- Titrated with Na2S2O3 :
become turbid white soluion
- (+) starch solution 1% +
Titrated with Na2S2O3 : form
2 layer, upper (colorless),
lower (white)
- Volume of Na2S2O3 0,1N
V1 = 1,9 mL
V2 = 1,7 mL
V3 = 1,8 mL
VB = 1 mL
Nu. Procedure Observationt Result Reaction Conclusion
2. Determine FFA Before Free fatty acid shows the flaw of oil The FFA
- Palm oil : yellow quality as long as much of free fatty acid, value is
6 grams of sample
- Alcohol : colorless the peroxide nmber bigger 0,0709%
- PP indicator : colorless
Inserted into erlenmeyer
- NaOH solution : colorless CH3(CH2)7CH = CH(CH2)7 COOH +
Added 10 mL of alcohol 96%
After CH3CH2OH
Added 5 drops of PP indicator - Palm oil + alcohol : formed CH3(CH2)7CH = CH(CH2)7 COOCH2CH3 +
Titrated with 0,1N NaOH solution two layer, upper (yellow), H2O (l)
until the color is red and not change for lower yellow (+)
30 second - Palm oil + alcohol + PP CH3(CH2)7CH = CH(CH2)7 COOCH2CH3 +
Calculated the % FFA indicator : formed two layer, NaOH
% Free Fatty Acid (FFA) upper (yellow), lower yellow CH3(CH2)7CH = CH(CH2)7 COONa +
CH3CH2OH
(+)
- After titrated with NaOH
become soft pink
V1 = 0,2 mL
V2 = 0,3 mL
V3 = 0,3 mL
Nu. Procedure Observation Result Reaction Conclusion
Blanco for FFA Before
6 grams of aquadest - Aquadest : colorless
- Alcohol : colorless
Inserted into erlenmeyer - PP indicator : colorless
Added 10 mL of alcohol 96% - NaOH solution : colorless
Added 5 drops of PP indicator After
- Aquadest + alcohol :
Titrated with 0,1N NaOH solution until the
colorless solution
color is red and not change for 30 second
- Aquadest + alcohol + PP
Calculated the % FFA
indicator : colorless solution

% Free Fatty Acid (FFA) - After titrated with NaOH

become soft pink
VB = 0,1 mL