Endamoeba coli

Overview of Endamoeba coli: Amoebiasis is defined as infection with the protozoan parasite
Entamoeba histolytica. However there are several Endamebas species which are closely related
to E. histolytica such as E. dipar and E. coli. (Anonymous 1997)

Entamoeba coli are a non-pathogenic species of Entamoeba that frequently exists as

a commensal parasite in large intestine of the human. Both cysts and trophozoites of the species
are passed in stool and considered diagnostic (CDC 2009). Cysts are typically found in formed
stool, whereas trophozoites are typically found in diarrheal stool. Colonization of the
nonpathogenic amebae occurs after ingestion of mature cysts in fecally-contaminated food,
water, or fomites(CDC 2009).

Summary of data:

Rendtorff, R. C. (1954). "The Experimental Transmission of Human Intestinal Prozoan

Parasites.I. Endamoeba coli Cyst Given in Capasules." American Journal of Hygien 59: 196-208-
studied the Entamoeba coli exposure to human volunteers (Rendtorff 1954). Known numbers of
cysts of E. coli were administered orally in capsules to volunteers to determine numbers of
parasites necessary to establish the infection

Table 1. Summary of data for Entamoeba coli

Experiment Host Type/Pathogen Route/ Dose Best Fit Optimized

Number
Reference Response ID50
Strain # of Doses Units Model Parameter(s)
= 0.1008
(Rendtorff No. of
1 Humans Ingestion 5 Infection Exponential N50 = 341.03 341.03
1954) cysts
Optimized Models and Fitting Analyses

Optimization Output for experiment 1

Table 2. Humans model data

Table 3. Best fitting model determination
Dose Infected Not infected Total
Model Deviance 20.95,1
10000 2 0 2
Exponential 27.82 3.8
1000 0 2 2 22.78
Beta Poisson 5.04
100 2 2 4
Beta-Poisson is best fitting model
10 3 7 10

1 1 7 8

(Rendtorff 1954)

Table 4 Optimized parameters for the best fitting (Exponential), obtained from 10,000 bootstrap iterations

Percentiles
MLE
Parameter
Estimate
0.5% 2.5% 5% 95% 97.5% 99.5%

0.00108 0.0117 0.0259 0.037 0.2125 0.2597 0.3075

N50 341.03 11.065 18.837 30.496 149244.7 2141374 1.5e7

ID50 341.03 0.007819 0.093663 0.100392 0.007819 13.25071 67.90096

Figure 1 Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of
the parameters

Figure 2 Beta-Poisson model plot, with confidence bounds around optimized model
Anonymous (1997). "Entamoeba taxonomy." Bulletin of the World Health Organization 75(3):
291-292.

CDC (2009). Intestinal Amebae. D. o. P. Diseases.

Rendtorff, R. C. (1954). "The Experimental Transmission of Human Intestinal Prozoan

Parasites. I. Endamoeba coli Cyst Given in Capasules." American Journal of Hygien 59:
196-208.