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Practical insight in artificial

reproduction and larval rearing


methods at Research Institute for
Fisheries and Aquaculture NARIC
HAKI

Uro Ljubobratovi1, Gza Pter1, Zoltn Horvth2, Andrs Rnyai1

1Research Institute for Fisheries and Aquaculture NARIC HAKI, H-5540


Szarvas, Anna liget 8, Hungary

2 H&H Carpi Halszati Kft., H-7814 csrd, Kossuth u. 7., Hungary

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NARIC HAKI
Lucioperca

Aquaexcel

Aquaexcel2020

GOODFISH (GINOP-2.3.2-15-2016-00025)

Nationally funded projects

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H&H Carpio Fishfarming Ltd.

Work started in 2010. After first results achived


from pond reared juveniles commercial production
started in 2013.
2015 cooperation started with HAKI, still 100 % of production from pond reared
juveniles In 2016, 26 % of juvenile prod. was from larvae In 2017, 76 % of
prod. was from larvae technology seems promising, but the outcome is still
unstable.

What we have:
Good technology (know how, facility) for weaning pond reared juveniles.
Tagged broodstock from 2014-2015.
Promising technology for larvae rearing in cooperation with HAKI

Future prospects, developements:


Shifting production to 100 % larvae production
Development of own feeders for live feed and micro feeds for better larvae production
Hatchery to start production in 2018-2019

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EPFC workshop 2017 New Skillsets in Percid Fish Culture
Comments Tasks
Impractical and laborious Practical

Unstable and unreliable Stable and repeatable

Hard and complicated Transmissible

Impossible unfeasible Feasible


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Artificial reproduction + larviculture

Robert Summerfelt
Daniel Zarski
et al.
et al.

Vitellogenesis Maturation Gametes Larvae

Sagiv Kolkovski
et al.

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1. Maturation temperature

12 6h
14 4h Ovulation

16 2h
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2. Hormonal application
hCG 500 IU kg-1 Perfect
conditions

sGnRHa 50 g kg-1 Critical


conditions

Which hormone to use?

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3. Maturation monitoring

Zarski et al., 2012


I 72 hours

II and III 48 hours

IV and V 24 hours

Further on every day until


stadium VI
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4. Suture
Needle 40 mm, 1/2 or 3/8, round or triangle section

Thread coated braids 2/0-1/0, monofilaments 1- 2

Method by Zarski et al. (2015)

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Percid Fish Culture
5. Gamete collection
Stripping upon eggs disposal occasionally
tricky recognition of full ovulation
Sperm collection catheterization

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6. Fertilisation and egg de-adhesion
Dry method 0.5-1 ml of sperm
per 100g of eggs
Fertilisation 2 minutes in plain
water

Egg washing in milk solution


during the egg swelling period

Finish with short kaolin bath

Procedure rather long research


to develop shorter reliable
procedure
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7. Egg incubation and hatching
Temperature 14-15 C
Oxygen 110-120 %
Incubation 7-8 days
Prophylaxis nothing or
formalin
Hatching upon eye
pigmentation
Eggs counting volumetrically
Synchronized hatching

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Larvae

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Environment

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Period until swim bladder
inflation
SBI
Swim
bladder
Period after swim bladder
inflation
POST-SBI

Month-old juvenile
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SBI period
1 -14 DPH
Stocking density 50-100 larvae l-1
Temperature 16 C
Flow up-welling 30 50 %
exchange per hour
Light 14:10 LD 10-15 lux day, 1
lux night
Feeding Artemia nauplii 100-300
nauplii larvae-1 day-1
Sprayer flat fan nozzle with
intensity adjustment

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POST-SBI period
15-30 DPH
Temperature increase from 16-19
during weaning
Flow circular 75-200% exchange
per hour
Light constant 30-40 lux until 25
DPH
Weaning period 15-20 DPH
Artemia reduction 20% per day
Dry feed Otohime B1, B2, C1
80-300 g m-1 day-1
31 DPH harvest
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Results
Cultured breeders
Embryo survival 50 80 %

RAS Pond-in-pond
Larviculture efficiency 20 38 %

Embryo survival 75 90 %
Wild breeders
Larviculture efficiency 4 33 %

Larviculture yield 10 20 proper fish per litter

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Implementation
Stizonativ Ltd. Kecskemet

Training

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Fish Culture
Future tasks
R epeat R
efine

T ransmit

Full control

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Fish Culture
Wish yall!!!

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