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ExPASy ProtParam tool

ProtParam
User-provided sequence:

10 20 30 40 50 60
MAHNHRHKHK LMNTINIAKN DFSDIELAAI PFNTLADHYG ERLAREQLAL EHESYEMGEA

70 80 90 100 110 120

RFRKMFERQL KAGEVADNAA AKPLITTLLP KMIARINDWF EEVKAKRGKR PTAFQFLQEI

130 140 150 160 170 180

KPEAVAYITI KTTLACLTSA DNTTVQAVAS AIGRAIEDEA RFGRIRDLEA KHFKKNVEEQ

190 200 210 220 230 240

LNKRVGHVYK KAFMQVVEAD MLSKGLLGGE AWSSWHKEDS IHVGVRCIEM LIESTGMVSL

250 260 270 280 290 300

HRQNAGVVGQ DSETIELAPE YAEAIATRAG ALAGISPMFQ PCVVPPKPWT GITGGGYWAN

310 320 330 340 350 360

GRRPLALVRT HSKKALMRYE DVYMPEVYKA INIAQNTAWK INKKVLAVAN VITKWKHCPV

370 380 390 400 410 420

EDIPAIEREE LPMKPEDIDM NPEALTAWKR AAAAVYRKDK ARKSRRISLE FMLEQANKFA

430 440 450 460 470 480

NHKAIWFPYN MDWRGRVYAV SMFNPQGNDM TKGLLTLAKG KPIGKEGYYW LKIHGANCAG

490 500 510 520 530 540

VDKVPFPERI KFIEENHENI MACAKSPLEN TWWAEQDSPF CFLAFCFEYA GVQHHGLSYN

550 560 570 580 590 600

CSLPLAFDGS CSGIQHFSAM LRDEVGGRAV NLLPSETVQD IYGIVAKKVN EILQADAING

610 620 630 640 650 660

TDNEVVTVTD ENTGEISEKV KLGTKALAGQ WLAYGVTRSV TKRSVMTLAY GSKEFGFRQQ

670 680 690 700 710 720

VLEDTIQPAI DSGKGLMFTQ PNQAAGYMAK LIWESVSVTV VAAVEAMNWL KSAAKLLAAE

730 740 750 760 770 780

VKDKKTGEIL RKRCAVHWVT PDGFPVWQEY KKPIQTRLNL MFLGQFRLQP TINTNKDSEI

790 800 810 820 830 840

DAHKQESGIA PNFVHSQDGS HLRKTVVWAH EKYGIESFAL IHDSFGTIPA DAANLFKAVR

850 860 870 880 890

ETMVDTYESC DVLADFYDQF ADQLHESQLD KMPALPAKGN LNLRDILESD FAFA

References and documentation are available.

Number of amino acids: 894

Molecular weight: 100245.92

Theoretical pI: 7.35

Amino acid composition: CSV format

Ala (A) 101 11.3%
Arg (R) 42 4.7%
Asn (N) 41 4.6%
Asp (D) 43 4.8%
Cys (C) 12 1.3%
Gln (Q) 33 3.7%
Glu (E) 67 7.5%
Gly (G) 54 6.0%
His (H) 26 2.9%
Ile (I) 52 5.8%
Leu (L) 68 7.6%
Lys (K) 68 7.6%
Met (M) 27 3.0%
Phe (F) 37 4.1%
Pro (P) 37 4.1%
Ser (S) 41 4.6%
Thr (T) 44 4.9%
Trp (W) 19 2.1%
Tyr (Y) 24 2.7%
Val (V) 58 6.5%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%

(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 110

Total number of positively charged residues (Arg + Lys): 110

Atomic composition:

Carbon C 4486
Hydrogen H 7021
Nitrogen N 1233
Oxygen O 1298
Sulfur S 39

Formula: C4486H7021N1233O1298S39
Total number of atoms: 14077

Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 141010

Abs 0.1% (=1 g/l) 1.407, assuming all pairs of Cys residues form cystines

Ext. coefficient 140260

Abs 0.1% (=1 g/l) 1.399, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 40.84

This classifies the protein as unstable.

Aliphatic index: 82.46

Grand average of hydropathicity (GRAVY): -0.304

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