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International Journal of Universal Pharmacy and Bio Sciences 6(2): March-April 2017
KEYWORDS: ABSTRACT
The Zanthoxylum Rhetsa(Roxb.) DC.Is belongs with the family of
Zanthoxylumrhetsa,
Rutaceae. In this plant is the having the various chemical
Phytochemical Study,
constituents like terpenoid, xanthyletin and sesamin, alkaloids,
Flavonoids, Alkaloids.
flavonoids and sabinene etc. The chemical constituents are the
For Correspondence:
important in the medical activities of the plant. The various parts
K.Gopalasatheeskumar *
Address: (bark, fruits and spines) of the plants are undergoes the various
KMCH college of activities in the collected works. There is no studies on the stem
Pharmacy, KovaiEstate parts of this plant is not present, so that this study is aimed to that
,Kalapatti Road, the phytochemical studies of the various types of the stem extracts.
Coimbatore-641048, In this study aqueous extract, benzene extract and ethanolic extract
Tamil Nadu, India. of the stem part of Zanthoxylum Rhetsaare the used for the
phytochemical studies in this work. This work concluded that the
various extracts are having the various chemical constituents. In
this work is very useful to the further study of the stem part of
Zanthoxylumrhetsa.
INTRODUCTION:
The Zanthoxylum Rhetsa(Roxb.) DC. Is belongs with the family of Rutaceae. The synonym of this
plant is Budrunga. A genus of herbs is well distributed in India, Bangladesh, bhutan, china etc. In
Tamil this plant is known as Veerasingi. [1,13] This plant contains the many of the chemical sustances
like terpenoid, xanthyletin and sesamin, alkaloids, flavonoids and sabinene. The different parts of
this species have been used in traditional medicine. The bark is having diuretic actions and used to
[2]
management of dental caries. In India old medicine, the bark has been used to treat cardiac,
respiratory diseases, tooth infection, stomach infection and rheumatism. The fruits are used as spice
and the essential oil extracted after the fruits is known as Mullilam oil used as anti-inflammatory,
antiseptic, anticholera, diarrhoea, hypo-cholesterolemic, mosquito repellent and soothing agent for
dental caries.[3,4] The Kanikkars family makes a paste of hard spines arranged by rubbing them
against rock with water and applies the extract to the breast of a nursing mother to aid pain and also
to increase milk supply. In the Phillippines, the powdered bark mixed with oil is a best formula to
treat stomach ache. In adding, the bark decoction is similarly taken to treat chest pain and chewed
bark applied as remedy for snake bites. The various parts of the plant is used for the various
medicinal values, there is many evidence is there for the study of bark, fruits and spines. [5]The
chemical constituents of the plants are the very important for the medicinal activities and there is no
evidence for the study of the stem of this plant, so that in this study aimed for the primary
phytochemical tests for the stem extracts are performed. In this study is very useful to the further
study of the various activities present in this stem part of this plant. [11]
II. MATERIALS AND METHODS
2.1 Plant material
The stem of Zanthoxylum rhesta (Roxb.) DC. Were collected from the area of Kollimalai hills in
Erode district, Tamilnadu, India. The stem of Zanthoxylum Rhetsa(Roxb.) DC. The plant material
was authenticated from Plant Anatomy Research Centre, Chennai, Tamilnadu, India and a voucher
specimen no: PARC/2014/2089.
2.1.1 Extraction of Plant Material:
The stem material was dried in the shade for two months. Then shade dried plant was subjected to
get coarse powder. The coarse powders were subjected to Soxhlet apparatus by using various
solvent according to their polarity. [6,12]
2.1.2 Materials Requirement
Shade dried stem powder, Benzene extract AR, Ethanol Extract AR, Aqueous Extract AR with
0.25% Chloroform water.
III.RESULTS
The results of the various extracts are phytochemical tests are given in the table 1, table 2 and table
3. The table 1 contains the all the positive and negative tests for the chemical constituents and table
2 and table 3 are contain only of the positive results of the chemical constituents.
Table 1: Phtyo-Chemical Tests for Benzene Extract of Zanthoxylum Rhetsa(Roxb.)Dc.
Compound Test Observation Inference
Mayers reagent Yellow precipitate
Alkaloids Dragondroffs Orange brown
reagent precipitate
Hagers reagent Yellow precipitate Presence of alkaloids
Wagners reagent Reddish brown
precipitate
Tannic acid test A precipitate
Ammonia Pink flocculent
Reineckatetest precipitate
Carbohydrates Molischs test No purple ring is
Fehlings solution test No Red precipitate
Barfoeds test No red cupric oxide
Seliwanoffs test No rose color is
produced Absence of
Test for pentoses No red color is carbohtdrates
formed.
Osazone formation No formation of
test osazone
Amino acids Millons test No white precipitate Absence of amino
Ninhydrine test No violet color acids
Iodinated chloride No blue color
Cellulose test Absence of cellulose
Iodine solution test No blue- violet
Lignin Phloroglucinol test Pink color Presence of lignin
Saffranine solution Pink color
Volatile Oil Sudan III solution Red color Presence of volatile
Fixed oils and fats Soap formation test Formation of soap Presence of fixed oil
color
Fixed oils and fats Soap formation test No Formation of soap absence of fixed oils
The tables are explains various extracts are having various chemical constituents. The benzene
extract contains the chemical constituents like alkaloids, lignin, volatile oils, fixed oils, flavonoids,
pectin and steroids. The ethanolic extract shows the positive results on alkaloids, amino acids,
volatile oils, fixed oils, flavonoids, glycosides, mucilage and steroids. And the aqueous extract is
shows the positive results of amino acids, volatile oils, fixed oils, flavonoids, mucilage, proteins
and starch. These are the results are the essential for the further studies on the stem part of
ZanthoxylumRhetsa(Roxb.)Dc.
III. CONCLUSION
In this present study the various chemical tests are performed in the three types of extracts of stem
part of ZanthoxylumRhetsa(Roxb.)Dc is performed from the results of this study, concluded that the
various extracts (benzene, ethanol, aqueous) are the having various chemical constituents. This
study is important in the study of the stem of ZanthoxylumRhetsa(Roxb.)Dc in the further medicinal
activity studies.
ACKNOWLEDGEMENT
I thankful to the almighty for blessings in successful completion of this work, my special thanks to
Mr.S.Parthiban and I would like to thank my friendsV.SanishDevan, M.Sanjay, A.Jeevanantham.
My deepest heartfelt gratitude and indebtedness belong to my Parents and Sisters who showed love,
affection, encouragement and finally made it possible to complete this work.
REFFERENCES
1. Jyothi B Patil,Shivanna H, KrishnamoorthiHegde. Seed Source Variation on Fruit and Seed
Traits of Zanthoxylumrhetsa- a Medicinal Tree under High Exploitation in Central Western
Ghats, India.International Journal of Pharmacy & Life Sciences. 2014; 5(12): 4081-4085.