Equipo 1

Dosis RotaRod (10 min) RotaRod(20 min) RotaRod (30 min) Miorelajacin (10 min)Miorelajacin (20 min)
0 60 60 60 32 33
0 60 60 26 15 5
0.625 47 60 60 14 14
0.625 37 52 19 7 5
1.25 7 2 3 5 7
1.25 32 36 14 11 11
2.5 12 8 0 3 5
2.5 0 1 0 3 3
5 3 0 0 3 1
5 1 0 0 3 2

0 0.625 1.25 2.5 5

0 60 60 60 60 60
10 60 42 19.5 6 2
20 60 56 19 4.5 0
30 60 39.5 8.5 0 0
S =
EEM
0S Eem 0.625
0 60 60
10 60 0 0 42
20 60 0 0 56
30 60 0 0 39.5

Curso temporal de Diacepam

Coordinacin motora
Efecto Modelo70 DE50 (mg/kg)
Coordinacin Hill 60 1.4
motora
Miorrelajacin Semilogartmico 1.11
50
Sedacin Semilogartmico <0.625
Tiempo en Rotarod (s) 40
Actividad motora - < 0.625
Ansioltico Semilogartmico
30 0.91 0.30
Anticonvulsionan
te 20

10

0
0 10 20 30

Tiempo (min)
0
0 10 20 30

Tiempo (min)
 Miorelajacin (30 min)Levantamientos Cuadros explorados Hoyos explorados C. Tnica C. Clnica
30 52 94 35 30 30
10 36 64 24 30 0:00
8 0 0 0
4 6 31 0 74 239
10 0 0 0
4 0 79 8
3 0 0 0 0 0
4 0 0
1 0 0 1 5 5
4 0 0 0 0 0

S EEM
0

S Eem 1.25 S Eem 2.5

60 60
7.0710678119 5 19.5 17.6776695297 12.5 6
5.6568542495 4 19 24.0416305603 17 4.5
28.9913780286 20.5 8.5 7.7781745931 5.5 0

e Diacepam
motora

0 mg/kg
0.625 mg/kg
1.25 mg/kg
2.5 mg/kg
5 mg/kg

20 30

(min)
 20 30

(min)
Muerte
85
1.25

0
0

S Eem 5S EEM
60
8.48528137 6 2 1.41421356 1
4.94974747 3.5 0 0 0
0 0 0 0 0
Equipo 1
Dosis Miorelajacin (10 Miorelajacin (20Miorelajacin (30 Miorelajacin (10 min)Miorelajacin (20 min)
0 32 33 30 32 32
0 15 5 10 15 15
0.625 14 14 8 14 14
0.625 7 5 4 7 7
1.25 5 7 10 5 5
1.25 11 11 4 11 11
2.5 3 5 3 3 3
2.5 3 3 4 3 3
5 3 1 1 3 3
5 3 2 4 3 3

0 0.625 1.25 2.5 5

0 30 30 30 30 30
10 23.5 10.5 8 3 3
20 19 9.5 9 4 1.5
30 20 6 7 3.5 2.5
S =
EEM
0S Eem 0.625
0 60 60
10 60 12.0208152802 8.5 42
20 60 19.7989898732 14 56
30 60 14.1421356237 10 39.5

Efecto Modelo DE50 (mg/kg)

Coordinacin Hill 1.4
motora
Miorrelajacin Semilogartmico 1.11
Sedacin Semilogartmico <0.625

Actividad motora - < 0.625

Ansioltico Semilogartmico 0.91 0.30
Anticonvulsionan
te
Miorelajacin (30 min)Levantamientos Cuadros explorados Hoyos explorados C. Tnica C. Clnica
32 52 94 35 30 30
15 36 64 24 30 0:00
14 0 0 0
7 6 31 0 74 239
5 0 0 0
11 0 79 8
3 0 0 0 0 0
3 0 0
3 0 0 1 5 5
3 0 0 0 0 0

S EEM
12.0208152802

S Eem 1.25 S Eem 2.5

60 60
4.9497474683 3.5 19.5 4.2426406871 3 6
6.3639610307 4.5 19 2.8284271247 2 4.5
2.8284271247 2 8.5 4.2426406871 3 0

Curso temporal de Diacepam

Miorrelajacin
35

30

25 0 mg/kg
Tiempo en alambre (s) 0.625 mg/kg
20
1.25 mg/kg
2.5 mg/kg
15
5 mg/kg
10

0
0 10 20 30

Tiempo (min)
5

0
0 10 20 30

Tiempo (min)
Muerte
85
1.25

0
0

S Eem 5S EEM
60
0 0 2 0 0
1.41421356 1 0 0.70710678 0.5
0.70710678 0.5 0 2.12132034 1.5
Equipo 1
Dosis Miorelajacin (10 Miorelajacin (20Miorelajacin (30 Miorelajacin (10 min)Miorelajacin (20 min)
0 32 33 30 32 32
0 15 5 10 15 15
0.625 14 14 8 14 14
0.625 7 5 4 7 7
1.25 5 7 10 5 5
1.25 11 11 4 11 11
2.5 3 5 3 3 3
2.5 3 3 4 3 3
5 3 1 1 3 3
5 3 2 4 3 3

0 0.625 1.25 2.5 5

10 44 6 0.5 0.5 0

S 11.313708499 0 0.7071067812 0.7071067812 0

EEM 8 0 0.5 0.5 0
0S Eem 0.625 E
0 60 60
10 60 12.0208152802 8.5 42
20 60 19.7989898732 14 56
30 60 0 0 39.5

Nmero de leva

Efecto Modelo DE50 (mg/kg)

Coordinacin Hill 1.4
motora
Miorrelajacin Semilogartmico 1.11
Sedacin Semilogartmico <0.625

Actividad motora - < 0.625

Ansioltico Semilogartmico 0.91 0.30
Anticonvulsionan
te
 Miorelajacin (30 min)Levantamientos Cuadros explorados Hoyos explorados C. Tnica C. Clnica
32 52 94 35 30 30
15 36 64 24 30 0:00
14 6 0 0
7 6 31 0 74 239
5 1 0 0
11 0 79 8
3 1 0 0 0 0
3 0 0
3 0 0 1 5 5
3 0 0 0 0 0

S EEM
12.0208152802

Curva Dosis-Respuesta
S Efecto Eem 1.25 S
sedante del Diacepam Eem 2.5
60 60
4.9497474683 55 3.5 19.5 4.2426406871 3 6
6.3639610307 4.5 19 2.8284271247 2 4.5
2.8284271247 45 2 8.5 4.2426406871 3 0

0 mg/kg
35 0.625 mg/kg
Nmero de levantamientos
1.25 mg/kg
25 2.5 mg/kg
5 mg/kg
15

-5

Dosis
Muerte
85
1.25

0
0

S Eem 5S EEM
60
0 0 2 0 0
1.41421356 1 0 0.70710678 0.5
0.70710678 0.5 0 2.12132034 1.5
Equipo 1
Dosis Miorelajacin (10 Miorelajacin (20Miorelajacin (30 Miorelajacin (10 min)Miorelajacin (20 min)
0 94 33 30 32 32
0 64 5 10 15 15
0.625 0 14 8 14 14
0.625 31 5 4 7 7
1.25 0 7 10 5 5
1.25 79 11 4 11 11
2.5 0 5 3 3 3
2.5 0 3 4 3 3
5 0 1 1 3 3
5 0 2 4 3 3

0 0.625 1.25 2.5 5

10 79 49 59 20 0

S 21.2132034356 25.4558441227 28.2842712475 28.2842712475 0

EEM 15 18 20 20 0
0S Eem 0.625
0 60 60
10 60 21.2132034356 15 42 Curv
20 60 19.7989898732 14 56
30 60 0 0
Efecto39.5en la ac
90

80

70

60
Efecto Modelo DE50 (mg/kg)
Nmero de
Coordinacin cruces 50
Hill 1.4
motora
40
Miorrelajacin Semilogartmico 1.11
Sedacin Semilogartmico <0.625 30

Actividad motora - < 0.625 20

Ansioltico Semilogartmico 0.91 0.30 10

Anticonvulsionan 0
te
 Miorelajacin (30 min)Levantamientos Cuadros explorados Hoyos explorados C. Tnica C. Clnica
32 52 94 35 30 30
15 36 64 24 30 0:00
14 0 67 0
7 6 31 0 74 239
5 0 39 0
11 0 79 8
3 0 40 0 0 0
3 0 0
3 0 0 1 5 5
3 0 0 0 0 0

S EEM
21.2132034356

S Eem 1.25 S Eem 2.5

60 60
21.9203102168 15.5
Curva Dosis-Respuesta 19.5 55.8614357137 39.5 6
6.3639610307 4.5 19 2.8284271247 2 4.5
ecto en la 2.8284271247
actividad motora2
del Diacepam
8.5 4.2426406871 3 0
90

80

70
0 mg/kg
60 0.625 mg/kg
ro de
ces 50 1.25 mg/kg
2.5 mg/kg
40 5 mg/kg
30

20

10

Dosis
Muerte
85
1.25

0
0

S Eem 5S EEM
60
0 0 2 0 0
1.41421356 1 0 0.70710678 0.5
0.70710678 0.5 0 2.12132034 1.5
Equipo 1
Dosis Miorelajacin (10 Miorelajacin (20Miorelajacin (30 Miorelajacin (10 min)Miorelajacin (20 min)
0 94 33 30 32 32
0 64 5 10 15 15
0.625 0 14 8 14 14
0.625 31 5 4 7 7
1.25 0 7 10 5 5
1.25 79 11 4 11 11
2.5 0 5 3 3 3
2.5 0 3 4 3 3
5 0 1 1 3 3
5 0 2 4 3 3

0 0.625 1.25 2.5 5

10 29.5 27.5 12.5 0.5 0

S 7.7781745931 6.3639610307 6.3639610307 0.7071067812 0

EEM 5.5 4.5 4.5 0.5 0
0S Eem 0.625 Curv
0 60 Actividad
60
10 60 21.2132034356 15 42
20 60 19.7989898732 14 56 35
30 60 0 0 39.5
30

25
Nmero de
exploraciones 20

Efecto Modelo DE50 (mg/kg) 15

Coordinacin Hill 1.4
motora 10
Miorrelajacin Semilogartmico 1.11
Sedacin Semilogartmico <0.625 5

Actividad motora - < 0.625 0

Ansioltico Semilogartmico 0.91 0.30
Anticonvulsionan
te
 Miorelajacin (30 min)Levantamientos Cuadros explorados Hoyos explorados C. Tnica C. Clnica
32 52 94 35 30 30
15 36 64 24 30 0:00
14 0 67 32
7 6 31 23 74 239
5 0 39 17
11 0 79 8
3 0 40 1 0 0
3 0 0 0
3 0 0 0 5 5
3 0 0 0 0 0

S EEM
21.2132034356

Curva Dosis-Respuesta
S Eem 1.25 S Eem 2.5
Actividad ansioltica del Diacepam 60 60
21.9203102168 15.5 19.5 55.8614357137 39.5 6
35 6.3639610307 4.5 19 2.8284271247 2 4.5
2.8284271247 2 8.5 4.2426406871 3 0
30

25 0 mg/kg
ero de 0.625 mg/kg
aciones 20 1.25 mg/kg
2.5 mg/kg
15 5 mg/kg

10

Dosis
Muerte
85
1.25

0
0

S Eem 5S EEM
60
0 0 2 0 0
1.41421356 1 0 0.70710678 0.5
0.70710678 0.5 0 2.12132034 1.5
Dosis RotaRod (10 min) RotaRod(20 min) RotaRod (30 min)
0 60 60 60
0
0.625 47 60 60
0.625
1.25 7 2 3
1.25 32 36 14
2.5 12 8 0
2.5 0 1 0
5 3 0 0
5
Dosis Miorelajacin (10 min)Miorelajacin (20 min)Miorelajacin (30 min)
0 32 33 30
0
0.625 14 14 8
0.625
1.25 5 7 10
1.25 11 11 4
2.5 3 5 3
2.5 3 3 4
5 3 1 1
5
Dosis Levantamientos
0 52
0
0.625
0.625
1.25
1.25 0
2.5 0
2.5
5 0
5
Dosis Cuadros explorados
0 94
0
0.625
0.625
1.25
1.25 79
2.5 0
2.5
5 0
5
 Dosis Hoyos explorados Promedio S EEM
0 35 29.5 7.77817459 5.5
0 24 0
0.625 0 0 0 0
0.625 0 0
1.25 0 4 5.65685425 4
1.25 8 0
2.5 0 0 0 0
2.5 0 0
5 1 0.5 0.70710678 0.5
5 0

Exploracione% No efecto % Efecto

Promedio Control 29.5 100
0.625 0 0 100
0.625 0 0 100
1.25 0 0 100
1.25 8 27.1186440678 72.8813559
2.5 0 0 100
2.5 0 0 100
5 1 3.3898305085 96.6101695
5 0 0 100
35

30

25
0 mg/kg
20 0.625 mg/kg
1.25 mg/kg
15 2.5 mg/kg
5 mg/kg
10

0
 Control 0.625 mg/kg 1.25 mg/kg 2.5 mg/kg 5 mg/kg 5 mg/kg
C. Tnica 30 74 0 290 0 5
C.Clnica 30 239 0 1084 0 5
Muerte 85 0 0 0 0 0

1
0.9
0.8
0.7
0.6
0 mg/kg
0.5 0.625 mg/kg
2.5 mg/kg
0.4
5 mg/kg
0.3
0.2
0.1
0
1 2 3 4
 S EEM

0 #DIV/0!
T ie m p o d e la t e n cia (s e g)

Efecto anticonvulsionante

C.
Tnica
0 mg/kg C.
0.625 mg/kg Clnica
2.5 mg/kg
5 mg/kg

Control0.625 mg/kg
1.25 mg/kg2.5 mg/kg 5 mg/kg 5 mg/kg
Dosis