ESRinterfacing Manual

Doc. No: D195.07.02 Rev.
01
Last revision: 10.09.2008
TEST1
ROLLER 20
ROLLER 10/10PN
ROLLER 20PN
INTERFACING MANUAL
REVISIONS
Revision Date Author Software Software Software Comments

n TEST1 ROLLER20 ROLLER10/20PN
00 08/07/2005 AP 4.05x 5.02 Creation
01 09/07/2008 AP 601F 6.01C R10-01.00P ROLLER10/20PN INTEGRATION
INTERFACING LATEX PROTOCOL
CONTACT ADDRESS
MANUFACTURER
SIRE Analytical Systems S.r.l.
Via Biella 121/3

33100 UDINE ITALY
tel. +39-0432/547454
fax +39-0432/547378
WORLDWIDE EXCLUSIVE DISTRIBUTOR
ALIFAX s.p.a.
Alifax S.p.A.
via Petrarca, 2/1
35020 Polverara (PD) - Italy
Tel. +39 049 0992000
Fax +39 049 5855434
http://www.alifax.com
INDEX
1. INTRODUCTION------------------------------------------------------------------------------------------------------------------------------------------------------------------- 1
2. ADD PARTS to INSTALL-------------------------------------------------------------------------------------------------------------------------------------------------------- 1
3. USEFUL TOOLS FOR DEBUGGING---------------------------------------------------------------------------------------------------------------------------------------- 1
4. SYMBOLS --------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 1
5. TEST1 RS232 PORTS features: ---------------------------------------------------------------------------------------------------------------------------------------------- 2
6. SERIAL CABLE PIN CONNECTIONS TO CONNECT TEST1 TO PC-LAB: ------------------------------------------------------------------------------------- 2
7. TEST1 protocol options ---------------------------------------------------------------------------------------------------------------------------------------------------------- 3
8. RS232 test function to check the communication of TEST1----------------------------------------------------------------------------------------------------------- 4
9. QUERY PROTOCOL project for TEST1 ------------------------------------------------------------------------------------------------------------------------------------ 5
10. Protocol description: -------------------------------------------------------------------------------------------------------------------------------------------------------------- 7
11. ROLLER20 (old model) RS232 PORTS features:------------------------------------------------------------------------------------------------------------------------ 9
12. SERIAL CABLE PIN CONNECTIONS TO CONNECT ROLLER20 TO PC-LAB: ------------------------------------------------------------------------------- 9
13. ROLLER 20 protocol options --------------------------------------------------------------------------------------------------------------------------------------------------10
14. RS232 test function to check the communication of ROLLER20 (OLD MODEL)-------------------------------------------------------------------------------11
15. QUERY PROTOCOL project for ROLLER20. ----------------------------------------------------------------------------------------------------------------------------13
16. Protocol description: -------------------------------------------------------------------------------------------------------------------------------------------------------------17
17. ROLLER 10- 10PN - 20PN RS232 PORTS features: ------------------------------------------------------------------------------------------------------------------19
18. SERIAL CABLE PIN CONNECTIONS TO CONNECT ROLLER10PN/20PN TO PC-LAB: -----------------------------------------------------------------19
19. ROLLER10PN/20PN PROTOCOL OPTIONS: ---------------------------------------------------------------------------------------------------------------------------20
20. RS232 test to check the communication of ROLLER10PN/20PN --------------------------------------------------------------------------------------------------22
21. QUERY PROTOCOL project for ROLLER 10- 10PN / ROLLER20PN. -------------------------------------------------------------------------------------------23
22. Protocol explanation (BCI Configuration): ---------------------------------------------------------------------------------------------------------------------------------24
23. TEST1, ROLLER10PN-ROLLER20PN and ROLLER20(old model) Multiple connections ------------------------------------------------------------------26
24. INTERFACING LATEX PROTOCOL----------------------------------------------------------------------------------------------------------------------------------------27
LATEX PROTOCOL INTERFACE EXPLICATION Up to Sw. version 6.01C -----------------------------------------------------------------------------------27
LATEX PROTOCOL INTERFACE EXPLICATION From Sw. version 6.01D (Test 1 and new Roller 10-10PN / 20PN) ----------------------------28
Note: The paragraphs written with the italic blue characters (as on this note) indicate a variation or an
addition to the previous version of the manual.
1. INTRODUCTION
This manual is focused to give detailed information about serial ports connections available on TEST1
ROLLER20 (old model) ROLLER10/10PN/20PN instruments and communication protocol features.
It should help Software House Companies during the modification or the installation of their program in the
Host Computer.
COMMON PARTS
2. ADD PARTS to INSTALL

A standard cable to connect two Personal Computers via serial port.
This cable must have:
D-type 25 pin female connector at one side and a D-type 9 pin female connector at the
opposite side for TEST1 and ROLLER20 (OLD MODEL).
D-type 9 pin female connector at one side and a D-type 9 pin female connector at the
opposite side for ROLLER10PN/20PN.
3. USEFUL TOOLS FOR DEBUGGING

A PC computer with a communication program installed (like HyperTerminal of Windows) to test the
instrument communications.
HyperTerminal allows the technician to check the communication messages byte by byte.
To run it follow the path: start, all programs, accessories, communication, HyperTerminal; run it and
choose new connection. Type a preferred name e.g. TEST1, press OK and then EXIT.
Open the curtain menu pressing File on the left upper side of HyperTerminal window and Property; a new
window will be opened. Press Connection and choose the serial port where the serial cable has been
connected: e.g.Com1.
Press Configuration and choose the speed which should be 9600Bps. Press OK twice.
4. SYMBOLS
The below symbols will be displayed on HyperTerminal if the program is configured with Terminal character.
= STX symbol
= ETX symbol
= ACK symbol
= NAK symbol
CHECKSUM = XOR calculation of part of R received message to check the correctness of it.
1
5. TEST1 RS232 PORTS features:
Communication ports parameters are fixed and cannot be modified.
Baud rate 9600
Parity N
Data bit number 8
Stop bit 1
HyperTerminal program must have the same parameters to a correct data exchanging.
PORT2 connection.
This instrument communicates through Serial Port 2. This

port uses a 25 pin male (DB25) serial connector. The used
pins are: no. 2 for TXDATA, no. 3 for RXDATA and 7 for
ground. The remaining pins are connected together as null-
modem configuration (4-5-8 and 6-20).
PORT1 connection
Serial Port 1 cannot be used for communication. It is connected

internally to the INTERNAL BAR CODE READER (IBCR if installed)
or it can be connected to an EXTERNAL BAR CODE READER (EBCR)
The maximum number of ASCII characters for each
patient ID is 15 (fifteen).
6. SERIAL CABLE PIN CONNECTIONS TO CONNECT TEST1 TO PC-LAB:

DB9 F DB25 F
1 1
2 2
3 3
4 4
5 5
6 6
7 7
8 8
9 9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
2
7. TEST1 protocol options
The right choice of the protocol enables the Query functionality.
The table below specify different configurations:
PROTOCOL Query DESCRIPTION R message structure SYMBOLS NOTES

DAT8 No Patient ID codes, STX Checksum note
having length of
1 byte
characters of 8
R 1 byte Checksum value is the result of
digits at Not used 2 byte Exclusive OR (XOR) of all
maximum, could Identification no 8 byte characters of the message,
be read by an Not used 6 byte excluded STX, ETX and
Checksum. If the Checksum value
external bar code ESR result 4 byte
calculated by the analyzer is 03
reader (EBCR) Checksum 1 byte (corresponding to ETX), or 02
or they could be ETX 1 byte (corresponding to STX), or 00
introduced typing (corresponding to NULL), the
them manually. value will be automatically
TOTAL = 24 byte
The output replaced with 7F hex value.
message Software House must adopt the
structure will be same procedure when
followed by the calculating checksum of the
sedimentation strings received from the
curve date (like analyzer.
STD) if KINET
option in the
COMMUNICA-
TION menu is
enabled.

DAT15 Yes It works by an STX 1 byte Checksum note
internal (IBCR) R 1 byte
Checksum value is the result of
or external bar Workstation Exclusive OR (XOR) of all
characters of the message,
code reader number 2 byte excluded STX, ETX and
(EBCR). It Identification no 15 byte Checksum. If the Checksum value
accepts the Rack no 2 byte calculated by the analyzer is 03
maximum Position 2 byte (corresponding to ETX), or 02
(corresponding to STX), or 00
patient identity Cycle/Bayer (Note) 2 byte (corresponding to NULL), the
code with 15 ESR result 4 byte value will be automatically
characters of Checksum 1 byte replaced with 7F hex value.
length. This ETX 1 byte Software House must adopt the
same procedure when
protocol will be
calculating checksum of the
activated TOTAL = 31 byte strings received from the
automatically if analyzer.
an IBCR is Bayer note:
connected. This field is managed by Bayer
parameter on tech/communication
Menu:
If Bayer is 0, the field deals with
the number of Cycle otherwise if
Bayer is 1, the field will contain
'01' (hex 30 and 31) to maintain
the Bayer protocol compatibility.
DAT8 either DAT15 option can be choice accessing in the COMMUNICATION MENU passing through
TECH MENU. Press key 5 from MAIN MENU and keys 1 and subsequently 9 as password and then key 3.
If the internal bar code reader is installed in a TEST1 instrument, DAT15 will be forced automatically at powering
on.
In the TEST1 COMMUNICATION MENU, RS232 Protocol option can be recalled pressing key
1- DAT08 2- DAT15 This message will be displayed
Option (1) Enables the patient identification number length to 8 bytes.

Option (2) Enables the patient identification number length to15 bytes.
3
8. RS232 test function to check the communication of TEST1
It simulates one transmission of 10 TEST1 results to host computer through RS232 serial port (SERIAL PORT 2).
If DAT8 is chosen, a message with 8 ID digits will be sent to the Host.
Selecting DAT15, the ID digit number to send to the Host is going to be 15.
DAT8 test transmission and its explanation:
Hex Length Description Note1:

Field name Symbol
value Checksum note
STX 02 1 char The first field is the same to all ten strings
Checksum value is the result of
R 52 1 char The second field is the same to all ten strings Exclusive OR (XOR) of all
Workstation The third field is the same to all ten strings characters of the message,
2 char
Number excluded STX, ETX and
Patient ID The furth field is different from the others strings Checksum. If the Checksum
8 char
Number value calculated by the analyzer is
Rack no. 2 char The fifth field is the same to all ten strings 03 (corresponding to ETX), or 02
Position 2 char The sixth field is different from the others (corresponding to STX), or 00
Bayer (Note2) 2 char The seventh field is the same to all ten strings (corresponding to NULL), the
Result 4 char The eighth field is different from the others value will be automatically
Checksum The nighth field is different from the others. replaced with 7F hex value.
1 char Software House must adopt the
(Note1)
The tenth field is the same to all ten strings. same procedure when
ETX 03 1 char
Note : what above explained refers to the TEST strings received from the
Strings instrument send to host computer, this analyzer.
string is displayed above this table.
Note2: Bayer field remains

activated to , '01' (hex 30 and 31)
because it is only a test.
Total length 24 char
DAT15 test transmission and its explanation:
Hex Length Description Note1:

Field name Symbol
value Checksum value is the result of
Exclusive OR (XOR) of all
STX ( 02 1 char The first field is the same to all ten strings
R 52 1 char The second field is the same to all ten strings excluded STX, ETX and
Workstation The third field is the same to all ten strings Checksum. If the Checksum value
2 char
Number calculated by the analyzer is 03
Patient ID The furth field is different from the others (corresponding to ETX), or 02
15 char
Number strings (corresponding to STX), or 00
Rack no. 2 char The fifth field is the same to all ten strings (corresponding to NULL), the
Position 2 char The sixth field is different from the others value will be automatically
Bayer (Note2) 2 char The seventh field is the same to all ten strings replaced with 7F hex value.
The eighth field is different from the others Software House must adopt the
Result 4 char
same procedure when
Checksum The nighth field is different from the others.
1 char calculating checksum of the
(Note1)
strings received from the
The tenth field is the same to all ten strings.
ETX 03 1 char analyzer.
Note : what above explained refers to the TEST
Strings instrument send to host computer, this
string is displayed above this table. Note2: Bayer field remains activated
to , '01' (hex 30 and 31)
4
In the COMMUNICATION MENU, RS232 function can be recalled pressing .
COMMUNICATION This message will be displayed

RS232 TEST TX
9. QUERY PROTOCOL project for TEST1

This document describes the serial protocol (Query Protocol) used by TEST1 with the Internal Bar Code
Reader (IBCR). It queries HOST Computer through Serial Port 2 (RS232C).
This protocol is the same of QUERY TEST1 program; a Windows software designed to be loaded in a
Personal Computer (LAB PC) installed in a laboratory and connected to HOST through a network.
QUERY protocol uses two files to exchange data for the communication with HOST by using the LAB PC like
a bridge between TEST1 and HOST Computer.
Further information of QUERY TEST1 program software can be taken from the QUERY TEST1 installation
and using manual.
TEST1 Settings for a correct communication
COMMUNICATION MENU in the TECH MENU, allows the configuration of some parameters for the correct communication
protocol. The accessing in it is allowed by typing key 5 from MAIN MENU, keys 1 and subsequently 9 for the password
requesting and key 3.
COMMUNICATION
1. RS232 PROTOCOL
2. RS232 TEST
3. KINET. SETUP
4. SETUP [ * ]
5. ENABLE BAYER
6. MEASURE MISSING ID
7. TIME OUT UART
8. TEST1 NUMBER
9. CLEAR
5
The configuration for a correct Query protocol functioning is:
RS232 PROTOCOL= It has to be set to DAT15 (Working with an Internal Bar Code Reader it is forced automatically).
To access inside and select DAT15 press key 1 in the COMMUNICATION menu.
KINET. SETUP= It must be kept to 0. Press key 3 in the COMMUNICATION menu to access inside and select 0.
This parameter, when activated, sends a kinetic curve data for factory checking after having
transmitted R message.
SETUP [ * ]= Press key 4 in the COMMUNICATION menu to access inside the option and 1 to enable the
asterisk character transmission to the host. It works when a analysed sample gives a low value of
hematocrit (around 20% and less).
The position of it in the message is explained in the Warnings (next page).
ENABLE BAYER Press key 5 in the COMMUNICATION menu to access Bayer option and 1 to enable it to
guarantee the Bayer protocol specifications (field Cycle/Bayer = 01).
If Bayer protocol is not requested this option must be kept to 0 (disable).
MEASURE MISSING ID= Press key 6 in the COMMUNICATION menu to access to the option and key 1 to allow the
analysis of samples without any identification number or not readable by IBCR.
Keeping it to 0, these samples wont be analysed.
TIME OUT UART= Press key 7 in the COMMUNICATION menu to access this option to set the waiting time (from 2 to
99 seconds) if the increasing of the waiting time to receive T message and the confirmation byte
(ACK) for having received R message is requested. The maximum time to receiving T message
and the confirmation byte (ACK) for having received R message remain set to 2 seconds keeping
it to OFF. If within this time no message comes in, the instrument will go ahead. This option works
if ACK option, available pressing 9 in the COMMUNICATION menu is enabled.
Verify of the parameters setting in the flag list printing:
Pressing 6 in the TECH MENU, the flag list printing is going to be obtained like in the following example:
TEST1
UK ver. 6.01F
Jun 27 2008 xx:xx:xx.
TEST1 SN. xxx
# AVAILABILITY xxxx
# GLOBAL COUNT. xxxx
BoosterY 1.0000
Model Fact 1.0000 .
MIXING CYCLES 180
MIXING SPEED 1
Smart warning 2000
Instrument number N 1
RS232 DAT15
FLOW CONT. ON
OFFSET LEV. FLOW 556
T. 100 974
FS fact 1.0159
Miss. ID set. 1
Change ID 0
ACK set 0
Print setup 1
Magnif Set 1
Calib. setup 1
Bayer. setup 1
[ * ] setup 0
Kinet.setup 0
Mixer speed 1
Offset car. 0
Offset nee. 0
Washing time 20
Vs. 0
TimeOut 20
6
10. Protocol description:
1. The internal Scanner reads the bar code (patient ID) from the label stuck on the tube, the instrument creates
Q message and sends it to HOST computer through RS232
serial port (or to PC LAB PC if it is connected like a bridge). <STX>Q01123456789 0000a<ETX>
2. HOST computer verifies if the patient identity code is present in the working list and, accordingly, it replies with
"T" message. TEST1 waits to receiving it within two seconds and then it goes ahead.
If ACK option is off, TEST1 is going to remain waiting up to 2 seconds for receiving it, otherwise if it is on,
TEST1 is going to wait for all the set time (TIMEOUT from 2 to 99 seconds).
TIMEOUT UART time and ACK are selectable in the COMMUNICATION MENU.
T message is going to have the result field filled with 0001, if

<STX>T01123456789 0001e<ETX>
the sample requires the analysis. The result field will be filled with
0000 if the sample doesnt require the analysis.
<STX>T01123456789 0000d<ETX>
3. After measuring (24 seconds about), TEST1 sends R

message to host replacing the result field value (0001) <STX>R01123456789 0103050012f <ETX>
with the ESR read value which should be between 0002
and 0120 or negative.
Errors messages are reported like: -001 for SM (Sample <STX>R01123456789 010305-004l <ETX>
Missing) error, -002 for NR (Not Readable) error and
-004 for NF (No flow) error.
4. Host computer should send ACK confirmation byte ( symbol displayed on the HyperTerminal program) to
TEST1 for having accepted R message. TEST1 will ignore it, if ACK is off otherwise it will wait for all the set
time (TIMEOUT from 2 to 99 seconds) if ACK is OFF.
ACK = Hexadecimal code 06
Warnings:
1 If a patient identity (ID) read by the TEST1 Scanner doesnt find its own correspondent code in the Host
computer working list, T message will be replaced by <NAK> message (NAK Message) Hex code 21
( symbol displayed on the HyperTerminal program). The sample will be
however analysed and R message sent to host.
2 If a particular sample has a very low hematocrit (around 20% and less) and * option in the communication
menu is enabled, the result field of R message, sent
to the host, will contains an asterisk set in the first <STX>R01123456789 010305*015[ <ETX>
position on the left and its own ESR result
(i.e.
If the analysis of a particular sample generates an error and * option in the communication menu is enabled,
ESR result field will contain 0 in the first left position,
- (minus) in the second and the error code (i.e. <STX>R01123456789 0103050-04\ <ETX>
3 If the internal Scanner cannot read the patient ID and MISS ID option in the Communication setup is
0, Q message wont be created. The sample without its own patient identity wont be processed.
7
4 If the internal Scanner cannot read the patient ID and MISS ID option in the Communication setup is
1, Q message wont be created. The sample without its own patient identity will be, however,
processed and R message sent to host with an identity code generated by the instrument itself.
The new patient ID, in this case, will be composed by 10 characters: cycle number (from 01 to 99)
(2 characters), instrument s/n (4 characters), rack number (from 01 to 04- 2 characters) sample position in
the rack (from 01 to 15- 2 characters).
Example:
Instrument s/n Rack number
0307920106
Cycle number Sample position in the rack
A complete R string for the transmission could be:
[Workstation number] [Instrument s/n] [Rack number] [ESR] [Checksum]
<STX>R010307920106 0106030012f <ETX>
[Cycle number] [Sample position] [Cycle number (Note 2)]
8
11. ROLLER20 (old model) RS232 PORTS features:
Communication ports parameters are fixed and cannot be modified.
Baud rate 9600
Parity N
Data bit number 8
Stop bit 1
HyperTerminal program must have the same parameters to a correct data exchanging.
PORT2 connection.
This instrument communicates to host through Serial Port 2.This

port uses a 25 pin male (DB25) serial connector and the used
pins are: no. 2 for TXDATA, no. 3 for RXDATA and 7 for ground.
The remaining pins are connected together as null-modem
configuration (4-5-8 and 6-20).
PORT1 connection.
Serial Port 1 has been reserved for the connection of an

external SCANNER The maximum number of ASCII
characters for each patient ID is 15 (fifteen).
12. SERIAL CABLE PIN CONNECTIONS TO CONNECT ROLLER20 TO PC-LAB:

DB9 F DB25 F
1 1
2 2
3 3
4 4
5 5
6 6
7 7
8 8
9 9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
9
13. ROLLER 20 protocol options
The right choice of the protocol enables the Query functionality.
The table below specify the different configurations:

BCI Yes It works by an STX 1 byte Checksum note:
internal (IBCR) or R 1 byte
Checksum value is the result
external bar code of Exclusive OR (XOR) of all
Workstation number characters of the message,
reader (EBCR). It
2 byte excluded STX, ETX and
accepts up to 15
Identification n 15 byte Checksum. If the Checksum
characters of value calculated by the
maximum patient Rack number 2 byte
analyzer is 03 (corresponding
identity code. The Position 2 byte to ETX), or 02 (corresponding
instrument enables Cycle/Bayer 2 byte to STX), or 00 (corresponding
Query protocol ESR result 4 byte to NULL), the value will be
which enquires the Checksum 1 byte automatically replaced with 7F
host if a specific hex value.
ETX 1 byte
sample needs to be Software House must adopt
TOTAL = 31 byte the same procedure when
analyzed or not.
strings received from the
analyzer.
Bayer note: This field is

managed by Bayer parameter
on Tech Menu.
If Bayer is 0, the field deals
with the number of Cycle
otherwise if Bayer is 1, the
field will contain '01' (hex 30
and 31) to maintain the Bayer
protocol compatibility.
STD, DAT8 DAT15 or BCI protocol can be selected by software accessing in the SETUP menu.
In the SETUP MENU list, RS232 Protocol can be recalled by pressing or until the following
message is displayed:
SETUP
RS232 PROTOCOL Confirm the function by pressing
The following menu will be displayed:
1 - STD 2 - DAT08
3 - DAT15 4 BCI _
10
14. RS232 test function to check the communication of ROLLER20 (OLD MODEL)
It simulates one test transmission of 10 test results to host computer through RS232 serial port (SERIAL PORT 2).
If DAT8 is chosen, a message with 8 ID digits will be sent to the Host.
Selecting DAT15 or BCI, the ID digit number to send to the Host is going to be 15.
STD test transmission and its examination:
No test is available in STD test
DAT8 test transmission and its examination:
Hex Length Description Checksum note:

Field name Symbol
2 char
Patient ID The furth field is different from the others strings (corresponding to ETX), or 02
8 char
Number (corresponding to STX), or 00
Bayer The seventh field is the same to all ten strings replaced with 7F hex value.
(Note 2)
Result 4 char The eighth field is different from the others same procedure when
Checksum The nighth field is different from the others. calculating checksum of the
1 char
(Note 1) strings received from the
The tenth field is the same to all ten strings. analyzer.
ETX 03 1 char
Bayer note: Bayer field remains
Note : what above explained refers to the TEST
Strings instrument send to host computer, this activated to , '01' (hex 30 and 31)
string is displayed above this table. because it is only a test.
DAT15 test transmission and its examination:

Field name Symbol
2 char
Patient ID The furth field is different from the others strings (corresponding to ETX), or 02
15 char
Number (corresponding to STX), or 00
(Note 2)
Result 4 char The eighth field is different from the others same procedure when
1 char
ETX 03 1 char
Note : what above explained refers to the TEST Bayer note: Bayer field remains
Strings instrument send to host computer, this activated to , '01' (hex 30 and 31)
string is displayed above this table. because it is only a test.
11
BCI test transmission and its examination:

Field name Symbol
Workstation The third field is the same to all ten strings Checksum. If the Checksum
2 char
Number value calculated by the analyzer
Patient ID The furth field is different from the others strings is 03 (corresponding to ETX), or
15 char
Number 02 (corresponding to STX), or 00
2 char Software House must adopt
(Note 2)
Result 4 char The eighth field is different from the others the same procedure when
1 char
ETX 03 1 char
Note : what above explained refers to the TEST Bayer note: Bayer field remains
Strings instrument send to host computer, this activated to , '01' (hex 30 and
string is displayed above this table. 31) because it is only a test.
In the TECH MENU, RS232 function can be recalled pressing or for many times until the
following message is displayed.
COMMUNICATION
RS232 TEST TX Press to run the test
12
15. QUERY PROTOCOL project for ROLLER20.
This paragraph describes the serial protocol (Query Protocol) used by ROLLER20 with an External Bar
Code Reader (EBCR) connected to Serial Port 2 (RS232C).
This protocol is the same used by QUERY TEST1 program; a Windows software designed to be loaded in a
QUERY protocol uses two files to exchange data for the communication with HOST by using the LAB PC like
a bridge between ROLLER20 and HOST Computer.
Further information of QUERY TEST1 program software can be taken from the QUERY TEST1 installation and
using manual.
Settings for a Query protocol correct communication on ROLLER20
The configuration for a correct Query protocol functioning is:
RS232 PROTOCOL= It must be set to BCI (And an External Bar Code Reader must be installed).
This function is available in the SETUP MENU. It can be recalled by pressing or
until the following message is displayed:
SETUP
RS232 PROTOCOL
Confirm the function by pressing
The following menu will be displayed:
1 - STD 2 - DAT08 Make your choice and press CLEAR

3 - DAT15 4 BCI _ twice to go back MAIN MENU
Chose 4 for a Query correct communication:
13
KINET. SETUP= This function is available in the SETUP MENU. It can be recalled by pressing or
SETUP
KINET. SETUP Confirm the function by pressing
The following message will be displayed:
KINET. SETUP= 0
1 -Y 0-N _
It must be set to 0
SETUP [ * ]= This function is available in the TECH MENU. It can be recalled by pressing or
TEC. CAL MENU 19

[ * ] SETUP Confirm the function by pressing
1- [ * ] [Y]
0- [ * ] [N]
The asterisk function activated allows the instrument to send the asterisk parameter to the
Host computer through the serial port. It will be displayed near the ESR value if the
sample analyzed has a haematocrit value low (< 20%).
IDENTITY ESR
Example of ESR result (045) with the * parameter enabled
and sent to the host computer with the patient identification
2367483493 *045 together.
IDENTITY ESR
Example of a sample analysed with a haematocrit value over
20% and * parameter enabled. The ESR result (5) will be
2367483493 0-05 sent to the host computer using this form.
Note:
Having disabled the asterisk function, the instrument cannot send the asterisk character to the host if the analysed
sample had a haematocrit value lower then 20% but it will be printed out next the ESR result however.
14
ENABLE BAYER Bayer communication protocol detects the Bayer field value (the previous field of the
ESR result) in the R message. If Bayer function in the COMMUNICATION menu is
enabled, the field will contain '01' (hex 30 and 31) to maintain the Bayer protocol
compatibility otherwise if it is disabled, the field will report the number of Cycle.
This function is available in the TECH MENU. It can be recalled by pressing or
TEC. CAL MENU 7

ENABLE BAYER Confirm the function by pressing
1- ENABLE [Y]
0- DISABLE [N]
Query setup= This function is available in the TECH MENU. It can be recalled by pressing or
TEC. CAL MENU 17

QUERY SETUP
1- Query [Y]
0- Query [N]
If Query is enabled (default), the instrument will be able to interrogate the Host computer
for the analysis requesting of each specific sample.
ACK= This function is available in the TECH MENU. It can be recalled by pressing or
TEC.CAL MENU 18
ACK SETUP Confirm the function by pressing
1 ACK [Y]
0 ACK [N]
This function allows to extend the waiting time for receiving T message (Query answer from the HOST computer)
and ACK (acknowledge message confirmation from the HOST computer for having received R message). To
modify the extension time (in seconds), it is available the TIMEOUT function explained in the Protocol description
(Paragraph 15).
15
Parameters verification:
In the SETUP menu you will find FLAG LIST function. Press or until the following message is
displayed:
SETUP
FLAGS LIST
Below is an example
ROLLER 20
UK ver. 6.01C
ROLLER20 SN. xxx
# AVAILABILITY xxxx
# GLOBAL COUNT. xxxx
GAIN 1.0000
MIXING CYCLES 180
MIXING SPEED 1
RS232 BCI
FLOW CONT. ON
OFFSET LEV. FLOW 800
OT OFFSET LEV. 700
T. 100 974
ROLLER n. 1
ACK setup 0
Query setup 1
Print setup 1
Calib. setup 1
Bayer. setup 1
[ * ] setup 0
Kinet.setup 0
Offset nee. 0
Vs 0
TimeOut 20
Warning SMART 1000
16
16. Protocol description:
1. The patient bar code ID stuck on each sample tube can be
read by using of an External Bar Code Reader connected at <STX>Q01123456789 0000a<ETX>
the ROLLER Serial Port1. The instrument will create and send
Q message to the HOST computer through RS232 serial port (or to LAB PC if used like a bridge) if Query
Setup option is 1. (Note 1)
2. HOST computer will verify if the patient identity has been inserted in the working list and then it replies with
"T" message.
If ACK option (in the TECH MENU list) is 0, ROLLER 20 remains waiting up to 2 seconds for receiving it.
otherwise if ACK option is 1 it will wait for all the set time (TIMEOUT from 2 to 99 seconds).
TIMEOUT UART time option is located in the TECH MENU list. (Note 2)
As soon as the instrument receives T message, it will be checked.

The wheel will rotate to one position to allow the operator to
insert the sample tube, if T message has the result field <STX>T01123456789 0001e<ETX>
compiled with 0001 (that means the sample requires the
analysis). The wheel remains stopped, if the result field will be <STX>T01123456789 0000d<ETX>
0000 (the analysis of that particular sample is not requested).
th
The procedure above explained will be repeated for the first to the 18 sample tubes before starting
the analysis.
3. After measuring (20 seconds about), ROLLER 20 will

<STX>R01123456789 0103050012F <ETX>
compile R messages replacing the result field value
(0001) with an ESR value between values of 0002 and
0120 or a negative one. For reading errors, the
instrument will report the error codes like: -001 for SM <STX>R01123456789 010305-004\ <ETX>
error, -002 for NR error and -004 for NF error.

4. Host computer should send an ACK confirmation byte ( symbol on hyper ACK Hex 06
terminal program) to ROLLER 20 for having accepted R message. The
instrument will ignore it, if ACK option is 0.
It will wait for all the set time (TIMEOUT from 2 to 99 seconds) if ACK option is 1.
Warnings:
1. If the patient identity (ID) read by the Scanner doesnt find its own correspondent in the Host computer
working list, T message will be replaced by <NAK> message Hexadecimal code 21
( symbol on hyper terminal program), the wheel will however rotate to the next
position to allow the operator to insert the tube. The sample will be analysed and R message sent to host.
2. If a particular sample has a very low haematocrit (less then 20%) and * option in the TECH MENU is
enabled, the result field of R message sent to the host will be composed by an asterisk in the first position
on the left and the rest its own ESR result (i.e. *015 ).
If the analysis of a particular sample generates an error, ESR result field will have 0 in the first left position
- (minus) for the second position and the rest of the field the error code (i.e. 0-04 ).
3. If no label has been stuck on the tube or the external Scanner cannot read the patient ID, Q
message wont be created. The wheel will rotate to accept the sample by pressing ENTER key
The sample will be however processed and R message sent to host with an identity code
generated by itself.
17
The new patient ID in this case will be composed by 10 characters:
cycle number (2 characters from 01 to 99), instrument s/n (4 characters), wheel number ( 2 characters)
sample position in the wheel (from 01 to 18- 2 characters).
Example:
Instrument s/n Wheel number
0307920106
Cycle number Sample position in the wheel
A complete R string for the transmission will be:
[Workstation number] [Instrument s/n] [Wheel number] [ESR] [Checksum]
<STX>R010307920106 0106030012I <ETX>
Note 1: If Query Setup option is 0, no Query protocol will be created; the instrument is going to accept and analyse all
samples.
Note 2: Follow the instruction looking at the Chapter 13-8 of the technical manual for TIMEOUT UART activation.
Note 3: This field is related to Bayer parameter on SETUP. If Bayer is 0, the field contains the number of Cycle. If Bayer is 1,
the field contains '01' (hex 30 and 31) to keep compatibility with Bayer protocol.
Note 4: Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and
Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to
STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value.
Software House must adopt the same procedure when calculating checksum of the strings received from the
analyzer.
18
17. ROLLER 10- 10PN - 20PN RS232 PORTS features:
PORT2 connection.
The instrument communicates through Serial Port 1.This port

uses a 9 pin male (DB9) serial connector.
The used pins are: n 2 for TXDATA, n 3 for RXDATA an d 7

for ground. The remaining pins are connected together as null-
modem configuration.
This cable must have a D-type 9 pin female connector from

one side and a D-type 9 pin female connector at the opposite
side.
PORT1 connection
Serial Port 1 has been reserved for the connection of an

external SCANNER The maximum number of ASCII
characters for each patient ID is 15 (fifteen).
18. SERIAL CABLE PIN CONNECTIONS TO CONNECT ROLLER10PN/20PN TO PC-LAB:

DB9 F DB9 F
1 1
2 2
3 3
4 4
5 5
6 6
7 7
8 8
9 9
19
19. ROLLER10PN/20PN PROTOCOL OPTIONS:
The choice of the protocol enables the Query functionality.
Patient ID codes, having length of characters of 8 digits at maximum, could be read by an external bar code reader (EBCR) or they could be
introduced typing them manually.
The tables below specify different configurations:
(NO QUERY) DAT8 transmission and its examination:

Checksum note:
Field name Symbol Hex value Length Description
See note at the end of this
page
STX 02 1 char Start transmission
R 52 1 char R states for result message Note2: Bayer field is not used, in
Workstation Is the number identifying the instrument if more than this case show s the
2 char progressive analysis cycle
Number one is connected to Host computer
Patient ID
8 char Patient identification
Number
Wheel no. 2 char Indicate the progressive number of analysis made
Position 2 char Indicate the sample position inside the wheel
Bayer 2 char
(Note 2) See note 2
Result 4 char ESR result
Checksum 1 char See note 1
(Note 1)
ETX 03 1 char End transmission

(NO QUERY) DAT15 transmission and its examination:

Note1:
page
R 52 1 char R states for result message Note2:see previous table
Workstation Is the number identifying the instrument if more than
2 char
Patient ID
Number
Rack no. 2 char Indicate the progressive number of analysis made
Bayer 2 char
(Note 2) See note 2
(Note 1)

(QUERY) BCI transmission and its examination:

Note1:
page
R 52 1 char R states for result message Note2:see previous table
Workstation Is the number identifying the instrument if more than
2 char
Patient ID
Number
Rack no. 2 char Indicate the progressive number of analysis made
Bayer 2 char
(Note 2) See note 2
(Note 1)

Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum
value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be
automatically replaced with 7F hex value.
Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer.
20
NO transmission:
NO Host No transmission
NO
In the Communication menu is possible to choose the appropriate protocol and options:
To activate the below option the password 1990 is compulsory.

From Main Screen, the path to enable it is Setup, Log In Out, LOGIN
A keyboard to type the password is going to be displayed. Type the password:1990 then press the left arrow to
confirm or Clear to exit without changing anything, in both cases, the instrument is going to display again the
previous image, then press Back.
21
20. RS232 test to check the communication of ROLLER10PN/20PN
To activate the below option the password 1990 is compulsory.
From Main Screen, the path to enable it is Setup, Log In Out, LOGIN
A keyboard to type the password is going to be displayed. Type the password:1990 then press the left arrow to
confirm or Clear to exit without changing anything, in both cases, the instrument is going to display again the
previous image, then press Back.
Pressing Comm menu and TEST it is possible to send a communication test to host computer through RS232
serial port.
If DAT8 is chosen, the test message having 8 ID digits will be sent to the Host like:
<0x2>R01TESTR10A0001010002U<0x3>
<0x2>R01TESTR10B0001020015S<0x3>
<0x2>R01TESTR10C0001030031U<0x3>
<0x2>R01TESTR10D0001040090^<0x3>
<0x2>R01TESTR10E0001050100V<0x3>
<0x2>R01TESTR10F0001060120T<0x3>
<0x2>R01TESTR10G000107-004N<0x3>
<0x2>R01TESTR10H000108-004N<0x3>
<0x2>R01TESTR10I000109-002H<0x3>
<0x2>R01TESTR10J000110-001@<0x3>
Selecting DAT15 or BCI, the ID digit number to send to the Host is going to be 15 like:
<0x2>R01TESTR10A 0101010002t<0x3>
<0x2>R01TESTR10B 0102010015r<0x3>
<0x2>R01TESTR10C 0103010031t<0x3>
<0x2>R01TESTR10D 0104010090<0x7f><0x3>
<0x2>R01TESTR10E 0105010100w<0x3>
<0x2>R01TESTR10F 0106010120u<0x3>
<0x2>R01TESTR10G 010701-004o<0x3>
<0x2>R01TESTR10H 010801-004o<0x3>
<0x2>R01TESTR10I 010901-002i<0x3>
<0x2>R01TESTR10J 011001-001a<0x3>
If NO HOST is configured, the instrument will not communicate with Host Computer
22
21. QUERY PROTOCOL project for ROLLER 10- 10PN / ROLLER20PN.
This paragraph describes the serial protocol (Query Protocol) used by ROLLER10PN /ROLLER20PN with an
External Bar Code Reader (EBCR) connected to Serial Port 1 (25 PIN).
This protocol is the same used by QUERY TEST1 program; a Windows software designed to be loaded in a
QUERY protocol uses two files to exchange data for the communication with HOST by using LAB PC like a
bridge between ROLLER10PN /ROLLER20PN and HOST Computer.
Further information of QUERY TEST1 program software can be taken from the QUERY TEST1 installation
and using manual.
Fields to check looking at

the flag list.
23
22. Protocol explanation (BCI Configuration):
1. The patient bar code ID stuck on each sample tube can be read
by using an External Bar Code Reader connected to Roller 10 <STX>Q01123456789 0000a<ETX>
Serial Port1. The instrument will create and send Q message
to HOST computer through RS232 serial port (or to LAB PC if it is used like a bridge) if Query Setup option
is 1. (Note 3)
2. HOST computer verifies if the patient identity has been inserted in the working list and then it replies with "T"
message. Roller 10 remains waiting for receiving it on time (within 2 seconds), if ACK option is disabled.
It will wait for all the set time if ACK option is enabled. (Follow the instruction looking on page 22 of this manual.)
3. As soon as the instrument receives T message, it is checked. <STX>T01123456789 0001e<ETX>
4. The wheel is going to rotate to one position to allow the operator
to insert the sample tube, if T message has the result field <STX>T01123456789 0000d<ETX>
compiled with 0001 (that means the sample requires the
analysis). The wheel will remain stop, if the result field is 0000 (the analysis of that particular sample is not
requested).
th
Before starting the analysis, the explained above, from 1 to 2, procedure will be repeated for the further 10
th
(Roller 10) or the 20 (Roller 20) sample tubes.
5. After measuring (20 seconds about), Roller 10 compile

<STX>R01123456789 0103050012f <ETX>
R messages replacing the result field value (0001)
with an ESR value that, read on the paper could be
between values of 0002 and 0120 or a negative one. <STX>R01123456789 010305-004l <ETX>
For reading errors, the instrument will report the error
codes like: --001 for SM error, -002 for NR error and -004 for NF error.
6. Host computer should send an ACK confirmation byte ( symbol looking at the
Hexadecimal code 0x06
hyper terminal program) to Roller 10 for having accepted R message. The
instrument will ignore it, if ACK option is disabled. It will wait for all the set time (TIMEOUT from 2 to 60
seconds) if ACK option is enabled.
Protocol explanation (DAT 8 DAT 15 Configuration):
In this case the instrument doesnt query the Host Computer, it just perform the analisis and send the result to host
computer as explaned at points 3 and 4.
Warnings:
1. If the patient identity (ID) read by the Scanner doesnt find its own correspondent in the Host computer
working list, T message will be replaced by <NAK> message ( symbol Hexadecimal code 0x21
looking at the hyper terminal program). If MISS ID option is enabled, the wheel
will rotate to the next position to allow the operator to insert the tube. The sample, then will be analysed and
R message sent to host.
2. If no label has been stuck on the tube or the external Scanner cannot read the patient ID, Q message wont
be created. The wheel is going to rotate to accept the sample tube by pressing Next button on touch-screen
display.
3. The sample is going to be processed and R message sent to host with an identity code generated by itself.
The new patient ID in this case will be composed by 10 characters:
4. Cycle number (2 characters from 01 to 99), instrument s/n (4 characters), wheel number ( 2 characters)
sample position in the wheel (from 01 to 10- 2 characters).
24
Example:
Instrument s/n Wheel number
0307920106
Cycle number Sample position in the wheel
A complete R string for the transmission will be:
[Workstation number] [Instrument s/n] [Wheel number] [ESR] [Checksum]
<STX>R010307920106 0106030012f <ETX>
Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding
to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value.
Software House must adopt the same procedure when calculating checksum of the strings received from
the analyzer.
Note 2: <STX> character (symbol looking at the hyper terminal program) in the serial transmission is represented by
0x02 hexadecimal bytes and <ETX> ( symbol looking at the hyper terminal program) by 0x03.
Note 3: If Query Setup option is 0, no Query protocol will be created; the instrument will accept and analyse all samples.
Note 4: Follow the instruction looking at the of the technical manual for TIMEOUT UART activation.
Note 5: This field is related to Bayer parameter on SETUP. If Bayer is 0, the field contains the number of Cycle. If Bayer is
1, the field contains '01' (hex 30 and 31) to keep compatibility with Bayer protocol.
25
23. TEST1, ROLLER10PN-ROLLER20PN and ROLLER20(old model) Multiple
connections
Query protocol is able to manage multiple connections by using the same PC-LAB with multiple serial ports.
In that case every instrument connected to the PC-LAB has to have an identification number different for each and
the PC-LAB the same number of serial ports as the instruments.
Our instruments have an option (TEST1 number in the COMMUNICATION menu (for TEST1) and ROLLER
number in the TECH MENU (for ROLLER20) to assign a different identification number to each instrument,
R message should be different for each like in the following example:
[Workstation number] [Checksum]
<STX> R010307920106 0106030012I <ETX>
<STX> R020308100106 0106030012z <ETX>
<STX> R030308150106 0106030012~ <ETX>
26
24. INTERFACING LATEX PROTOCOL
LATEX PROTOCOL INTERFACE EXPLICATION Up to Sw. version 6.01C (Test 1), 6.01B (Roller20 old
model)
The three R Latex messages are sent, to the serial port, after having analysed the three referent Latex of the
Control kit and at the end of the washing, by withdrawing water from the fifth and sixth inserted tube, for rinsing the
capillary.
It should be understood R Latex messages are not available by using Calibration Latex kit.
Messages
R message for Latex
Hex Length
Field name Description
value
STX (Note 2) 02 1 char
R 52 1 char Means the instrument is sending a Result
Identifies the workstation if more instrument are
Workstation Number 2 char
connected, normally is 01
LATEXSTDL 9 char Is the identification of the latex tube
Can be 1, 2 or 3 and identifies level 1, 2 and 3 of
Field 1 1 char
latex
ESR Value 3 char This is the expected Latex ESR value
Space. 20 2 char Two spaces
Field 2 2 char Fixed to 01
Field 3 2 char The same as Field 1
ESR Value 4 char This is the detected Latex ESR value
Checksum (Note 1) 1 char
ETX 03 1 char
the analyzer.
Note 2: <STX> character (symbol looking at the hyper terminal program) in the serial transmission is represented by 0x02 hexadecimal
bytes and <ETX> ( symbol looking at the hyper terminal program) by 0x03.
here are some examples:

<0x2>R01LATEXSTDL1005 0100010005^<0x3>
<0x2>R01LATEXSTDL2028 0101010029><0x3>
<0x2>R01LATEXSTDL3071 0102010072><0x3>
27
LATEX PROTOCOL INTERFACE EXPLICATION From Sw. version 6.01D (Test 1), 6.01C (Roller20 old model)
and 1.00P ( new Roller 10-10PN / 20PN)
When the instrument runs latex controls, it is possible to send the results to host computer, in this case the instrument will
send a specific string here explained:
R message for Latex

Hex Length
Field name Description
value
STX (Note 2) 02 1 char
R 52 1 char Means the instrument is sending a Result
Identifies the workstation if more instrument are
Workstation Number 2 char
connected, normally is 01
Is the identification of the latex and the Lot
LATEX0 XYZ 9 char
to which they belong
Can be 1, 2 or 3 and identifies level 1, 2 and 3 of
Field 1 1 char
latex
ESR Value 3 char This is the expected Latex ESR value
Space. 20 2 char Two spaces
Field 2 2 char Fixed to 01
ESR Value 4 char This is the detected Latex ESR value
Checksum (Note 1) 1 char
ETX 03 1 char
the analyzer.
here are some examples:
<0x2>R01LATEX08451005 0101010002<0x19><0x3>
<0x2>R01LATEX08452036 0102010034<0x1c><0x3>
<0x2>R01LATEX08453083 0103010071<0x13><0x3>
28

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Doc. No: D195.07.02 Rev.

Revision Date Author Software Software Software Comments

SIRE Analytical Systems S.r.l.

Via Biella 121/3

WORLDWIDE EXCLUSIVE DISTRIBUTOR

2. ADD PARTS to INSTALL

3. USEFUL TOOLS FOR DEBUGGING

This instrument communicates through Serial Port 2. This

Serial Port 1 cannot be used for communication. It is connected

6. SERIAL CABLE PIN CONNECTIONS TO CONNECT TEST1 TO PC-LAB:

PROTOCOL Query DESCRIPTION R message structure SYMBOLS NOTES

PROTOCOL Query DESCRIPTION R message structure SYMBOLS NOTES

1- DAT08 2- DAT15 This message will be displayed

Option (1) Enables the patient identification number length to 8 bytes.

DAT8 test transmission and its explanation:

Hex Length Description Note1:

Note2: Bayer field remains

DAT15 test transmission and its explanation:

Hex Length Description Note1:

COMMUNICATION This message will be displayed

9. QUERY PROTOCOL project for TEST1

TEST1 Settings for a correct communication

Verify of the parameters setting in the flag list printing:

T message is going to have the result field filled with 0001, if

3. After measuring (24 seconds about), TEST1 sends R

Instrument s/n Rack number

Cycle number Sample position in the rack

A complete R string for the transmission could be:

[Workstation number] [Instrument s/n] [Rack number] [ESR] [Checksum]

<STX>R010307920106 0106030012f <ETX>

[Cycle number] [Sample position] [Cycle number (Note 2)]

This instrument communicates to host through Serial Port 2.This

Serial Port 1 has been reserved for the connection of an

12. SERIAL CABLE PIN CONNECTIONS TO CONNECT ROLLER20 TO PC-LAB:

PROTOCOL Query DESCRIPTION R message structure SYMBOLS NOTES

Bayer note: This field is

The following menu will be displayed:

No test is available in STD test

DAT8 test transmission and its examination:

Hex Length Description Checksum note:

DAT15 test transmission and its examination:

Hex Length Description Checksum note:

Hex Length Description Checksum note:

Settings for a Query protocol correct communication on ROLLER20

The configuration for a correct Query protocol functioning is:

This function is available in the SETUP MENU. It can be recalled by pressing or

until the following message is displayed:

The following menu will be displayed:

1 - STD 2 - DAT08 Make your choice and press CLEAR

Chose 4 for a Query correct communication:

until the following message is displayed:

The following message will be displayed:

until the following message is displayed:

TEC. CAL MENU 19

The following message will be displayed:

This function is available in the TECH MENU. It can be recalled by pressing or

until the following message is displayed:

TEC. CAL MENU 7

The following message will be displayed:

until the following message is displayed:

TEC. CAL MENU 17

until the following message is displayed:

The following message will be displayed:

As soon as the instrument receives T message, it will be checked.