SPS4

Agilent SPS 4
Autosampler
User’s Guide
Agilent Technologies
Notices
No part of this manual may be reproduced in Technology Licenses
any form or by any means (including elec- CAUTION
tronic storage and retrieval or translation The hardware and/or software described in
into a foreign language) without prior agree- this document are furnished under a license A CAUTION notice denotes a haz-
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Restricted Rights Legend that, if not correctly performed or
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Warranty Agencies of the U.S. Government will tion on the usage - it does not
The material contained in this docu- receive no greater than Restricted Rights as
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law, Agilent disclaims all warranties, applicable in any technical data. user guides. Any other use may result in
either express or implied, with regard damage to the product or personal injury.
to this manual and any information Safety Notices Agilent is not responsible for any damages
contained herein, including but not caused, in whole or in part, by improper use
limited to the implied warranties of of the products, unauthorized alterations,
merchantability and fitness for a par- adjustments or modifications to the prod-
ticular purpose. Agilent shall not be
WA R N I N G ucts, failure to comply with procedures in
liable for errors or for incidental or Agilent product user guides, or use of the
consequential damages in connec-
A WARNING notice denotes a products in violation of applicable laws,
tion with the furnishing, use, or per- hazard. It calls attention to an rules or regulations.
formance of this document or of any operating procedure, practice, or Agilent shall not be liable for errors or dam-
information contained herein. Should the like that, if not correctly per- ages on products or performance due to
Agilent and the user have a separate using non-Agilent genuine parts.
formed or adhered to, could result
written agreement with warranty
terms covering the material in this
in personal injury or death. Do not
document that conflict with these proceed beyond a WARNING
terms, the warranty terms in the sep- notice until the indicated condi-
arate agreement shall control. tions are fully understood and
met.
Contents
Contents
1 Safety Practices and Hazards
Electrical Hazards 8
Flammable Liquids 9
Other Precautions 9
Safety Symbols 10
Warning Symbols 11
CE Compliance 12
Environmental Conditions 12
Electromagnetic Compatibility 13
2 Introduction
Overview 16
Front and Rear Views 17
Front View 17
Rear View 19
Intended Use 21
Specifications 22
Environmental 22
Power 22
Weights and Dimensions 23
Key Specifications 23
Options and Accessories 25
3 Installation
Installation Procedure 30
Orientation 30
Bench Requirements 32
Agilent SPS 4 Autosampler User’s Guide 3

Contents
Inspection and Unpacking 32

Unpacking and Lifting 32
Installing the Cover Kit 34
Fitting the Spill Tray 34
Installing the Rack Location Mat 36
Installing the Wash Reservoir 37
Installing the Standards Rack 39
Installing the Sample Racks 40
Fitting the Sample Probe 42
Fitting the Wash Pump Tubing 44
Two Drain Arrangements 46
Loading Wash Pump Tubing 47
Pressure Plate Adjustment 48
Connecting the Power and the Communications Cable 48
Confirming the Operation 50
Setting up the Instrument Controlling Software for SPS 4 50
Notes on Lifting the Autosampler 51
4 Operation
Starting up the Autosampler 54
Shutting Down the Autosampler 55
Flushing the Wash Reservoir and Flow Path 55
Software Control 56
5 Maintenance
Routine Maintenance 58
Cleaning 58
4 Agilent SPS 4 Autosampler User’s Guide

Contents
Replacing the Wash Pump Tubing 59

Lubrication 60
Service 60
Spare Parts 60
6 Troubleshooting
Troubleshooting Guide 64
Power Supply Problems 65
Operating State Problems 65
Error Indicator Codes 66
Initialization Problems 68
Probing Problems 69
Probe Arm Jam 69
When the Sample Probe Hits an Object 69
Communication Problems 70
7 Running the Calibration Wizard

Before User Calibration 72
Calibration Wizard Overview 72
Performing User Calibration 74
Error List 79
A Appendices
Introduction 82
Tools Required 83
Unpacking your Autosampler Cover 83
Installing your Autosampler Cover 84
Door Operation 91

Contents
Connections 92
Care and Maintenance 92
Preventing Earthquake Damage 94
Default Rinse Materials 95

Agilent SPS 4 Autosampler
User’s Guide
1
Safety Practices and Hazards
Electrical Hazards 8
Flammable Liquids 9
Other Precautions 9
Safety Symbols 10
Your Agilent SPS 4 autosampler has been carefully designed

so that when used properly you have an accurate, fast,
flexible and safe analytical system.
If the equipment is used in a manner not specified by
Agilent, the protection provided by the equipment may be
impaired.
Information about safety practices appears throughout the
documentation provided with your equipment. Before using
the equipment, ensure you read these safety practices
carefully.
Observe all relevant safety practices at all times.
Agilent Technologies 7
Electrical Hazards
The Agilent SPS 4 contains electrical circuits, devices, and components
operating at dangerous voltages. Contact with these circuits, devices and
components can cause death, serious injury, or painful electrical shock.
To reduce the risks of electrical shock, this equipment employs a
three- wire electrical cord and plug to ground the equipment. To use this
safety feature:
• Ensure the matching wall outlet receptacle is properly wired and
grounded correctly.
• Never use a three- wire to two- wire plug adapter.
• Never use a two wire extension cord or a non- grounded multiple outlet
receptacle strip.
• Do not use any power cord other than that supplied with this
equipment.
• The power cord set that is shipped with this equipment cannot be used
with other products.
• Position the autosampler so that it is easy to access the mains power
on the wall outlet or to remove the power cord from the autosampler or
wall outlet.
• Only Agilent- trained, Agilent- qualified, or Agilent- approved customer
support representatives may open panels or covers that are secured by
screws. Removal of such panels or covers can expose parts that involve
the risk of electric shock or personal injury.
This equipment is rated Equipment Class I.

NOTE
Application of the wrong supply voltage can create a fire hazard and a
potentially serious shock hazard, and can seriously damage the
autosampler.
Do not connect the autosampler to the mains power supply until all
checks and settings have been completed.
Replace or repair faulty or frayed insulation on power cords.

Safety Practices and Hazards 1
The autosampler must always be connected to protective earth through the power
WA R N I N G
cord. Position the autosampler so that it is easy to access the mains power switch
on the wall outlet or to remove the power cord from the autosampler or wall outlet.
Flammable Liquids
Observe the following precautions when using your autosampler in
conjunction with flammable liquids.
To reduce the risk of fire:
• Ensure the following autosampler parts do not contact the surface of
the fluid or have parts near the surface of the fluid:
• parts that consume power
• parts that produce heat
• parts that conduct heat from other areas in any form
• Ensure no parts in or near the fluid surface are capable of igniting a
flammable liquid.
• Operators must adhere to standard laboratory practices for the safe
handling of flammable liquids.
Other Precautions
Use of the autosampler in any manner other than that specified by Agilent
may impair the protection provided by the equipment.
Use of the autosampler may involve materials, solvents and solutions that
are flammable, corrosive, toxic or otherwise hazardous.
Careless, improper, or unskilled use of materials, solvents and solutions
can create explosions, fire, toxicity and other hazards that can result in
death, serious personal injury and damage to equipment and property.

Always ensure that laboratory safety practices governing the use, handling
and disposal of such materials are strictly observed. These safety practices
should include the wearing of appropriate safety clothing and safety
glasses.
Components of the autosampler move automatically and at speed.
Keep clear of the probe arm assembly during operation to prevent injury.
WA R N I N G
A protective cover is available to prevent accidental contact with the probe arm assembly.
NOTE Contact your local Agilent representative for further information.
Safety Symbols
The general definitions of safety symbols used on equipment or in
manuals are listed below.
Direct current.
^ On supply.
Off supply.
Hazardous voltage and potential for electrical shock. Do not touch
terminals that have this symbol when the equipment is on.
Caution, refer to accompanying documentation. Equipment marked with
this symbol indicates that it is necessary for the user to refer to the
instruction manual.
The CE mark shows that the product complies with all applicable
European Directives.
The CSA mark is a registered trademark of the Canadian Standards

Association.

The RCM mark is a registered trademark of the Australian

Communications Authority. This signifies compliance with the Australian
EMC Framework Regulations under the terms of the Radio
communications Act.
This ISM device complies with Canadian ICES- 001.
Cet appareil ISM est conforme à la norme NMB- 001 du Canada.
This is the symbol for an Industrial, Scientific and Medical, Group 1 Class
A product. (CISPR 11)
South Korean Certification (KC) mark.
Warning Symbols
The following is a list of symbols that appear in conjunction with
warnings in this manual or on the equipment. The hazard they describe is
also shown.
A triangular symbol indicates the warning type. The meanings of the
symbols that may appear alongside warnings in the documentation or on
the instrument itself are as follows:

CE Compliance
Your autosampler has been designed to comply with the requirements of
the Electromagnetic Compatibility (EMC) Directive and the Machinery
Directive (commonly referred to as the MD) of the European Union.
Agilent has confirmed that each product complies with the relevant
Directives by testing a prototype against the prescribed EN (European
Norm) standards.
Proof that a product complies with these directives is indicated by the CE
symbol on the rear of the product, and the documentation that
accompanies the product containing a copy of the Declaration of
Conformity. The Declaration of Conformity (DoC) is the legal declaration
by Agilent that the product complies with the directives listed above, and
shows the EN standards to which the product was tested to demonstrate
compliance.
Environmental Conditions
This equipment meets the following IEC classifications:
• Pollution Degree 2 (See Note.)
• Installation Category II (See Note.)
• Equipment Class 1
The "Pollution Degree" indicates the level to which a solid, liquid, or gas deteriorates
NOTE according to its dielectric strength. The "2" indicates a normal indoor atmosphere.
The "Installation Category" indicates the regulation for impulse withstand voltage and is
also known as the "Over Voltage Category". The "II" indicates electrical equipment.

Electromagnetic Compatibility
EN55011/CISPR11
Group 1 ISM equipment: group 1 contains all ISM equipment in which
there is intentionally generated and/or used conductively coupled
radio- frequency energy which is necessary for the internal functioning of
the equipment itself.
Class A equipment is equipment suitable for use in all establishments
other than domestic and those directly connected to a low voltage power
supply network which supplies buildings used for domestic purposes.
This device complies with the requirements of CISPR11, Group 1, Class A
as professional radiation equipment. Therefore, there may be potential
difficulties in ensuring electromagnetic compatibility in other
environments, due to conducted as well as radiated disturbances.
Operation is subject to the following two conditions:
1 This equipment may not cause harmful interference.
2 This equipment must accept any interference received, including
interference that may cause undesired operation.
If this equipment does cause harmful interference to radio or television
reception, which can be determined by turning the equipment off and on,
the user is encouraged to try one or more of the following measures:
1 Relocate the radio or antenna.
2 Move the equipment away from the radio or television.
3 Plug the equipment into a different electrical outlet, so that the
equipment and the radio or television are on separate electrical circuits.
4 Ensure that all peripheral equipment is also certified.
5 Ensure that appropriate cables are used to connect the equipment to
peripheral equipment.
6 Consult your equipment dealer, Agilent Technologies, or an experienced
technician for assistance.
Changes or modifications not expressly approved by Agilent Technologies
could void the user's authority to operate the equipment.

South Korean Class A EMC declaration

This equipment is Class A suitable for professional use and is for use in
electromagnetic environments outside of the home.
A 급 기기
( 업무용 방송통신기자재 )
이 기기는 업무용 (A 급 ) 전자파적합기기로서 판
매자 또는 사용자는 이 점을 주의하시기 바라
며 , 가정외의 지역에서 사용하는 것을 목적으
로 합니다 .
ICES/NMB-001
This ISM device complies with Canadian ICES- 001.
Cet appareil ISM est conforme à la norme NMB- 001 du Canada.
Sound Emission
This device complies with Machinery Directive 2006/42/EC.
Sound pressure Lp < 70 dB(A)

User’s Guide
2
Introduction
Overview 16
Front and Rear Views 17
Intended Use 21
Specifications 22
Options and Accessories 25
2 Introduction
Overview
The Agilent SPS 4 autosampler, represents the state- of- the- art in
autosampler design and is the latest generation of autosamplers from
Agilent. The Agilent SPS 4 is a random access, single probe autosampler,
providing front- end automation for several Agilent instruments.
The Agilent SPS 4 can accommodate a variety of fixtures, including a
standards rack, four sample racks, a wash reservoir, a sample probe and
other accessories.
Depending on your application, there is a choice of options. Note that
“diameters” in the following list mean outside diameters (OD):
• Standards Rack:
34 well rack (12 wells for 29 mm diameter tubes + 22 wells for 17 mm
diameter tubes)
5 well rack (for 61 mm diameter bottles)
• Flat Pack Sample Racks:
21 position rack for 30 mm diameter tubes
• Four 96- well microtiter plates with microtiter plate adapter
• Wash Reservoir: single or dual port
• Sample Probe: range of different probe types, probe materials and
internal diameters

Introduction 2
Front and Rear Views
Front View
The Agilent SPS 4 has 3 axes of movement for the sample probe —
longitudinal (X- axis), rotary or theta (T- axis) and up and down (Z- axis).
Figure 1 shows the front view of the Agilent SPS 4.
X-Axis 9
T-Axis
Z-Axis
7
4 3 2 1 5
Figure 1 Front view of the Agilent SPS 4
1 Spill Tray
The spill tray sits on the bottom base bars of the Agilent SPS 4, and
catches any spills.
2 Rack Location Mat
The rack location mat sits on top of the spill tray, and positions the
sample racks, standards rack and fixed wash reservoir.

2 Introduction
3 Standards Rack
The standards rack is located in the center of the rack location mat.
There are 2 standard rack options.
• 34 well (12 wells for 29 mm OD tubes + 22 wells for 17 mm OD
tubes)
• 5 well (for 61 mm OD bottles)
4 Sample Racks
The sample racks are located on both (left and right) sides of the
standards rack. The rack location mat has a maximum of four sample
rack positions — two rack positions on either side of the standards rack
position.
• 21 position rack for 30 mm OD tubes
5 Microtiter Plate Adapter
The microtiter plate adapter is used to mount four 96- well microtiter
plates on the rack location mat. It occupies two sample rack positions.
6 Fixed Wash Reservoir
The fixed wash reservoir is located at the rear of the rack location mat.
It is supplied with tubing used to connect the wash reservoir to the
rinse source and the waste container.
7 Probe Arm
The probe arm moves the sample probe on 3 axes — longitudinal
(X- axis), rotary or theta (T- axis) and up and down (Z- axis).
8 Sample Probe
The sample probe is mounted on the probe arm.
9 Power/Error Indicator
The power/error indicator is located on the front panel. The LEDs are
green to indicate power is on and the autosampler is functioning
correctly. The indicator lights change to red when an error occurs in
the equipment. There are 4 error indicators and these illuminate in
different combinations to indicate a specific error condition. See "Error

Introduction 2
Indicator Codes" on Page 66 for details of the various error codes.
Rear View
Figure 2 shows the rear view of the Agilent SPS 4.
6 5 4 3 2 1
Figure 2 Rear View of Agilent SPS 4
1 Power Switch
The power switch turns on/off the Agilent SPS 4.
I: Power is ON.
O: Power is OFF.
When the Agilent SPS 4 is turned on, the initialization sequence runs to
check the basic functions and set the probe position. If there are no
errors, the power/error indicator turns green.
2 Power Supply Input
The output connector from the AC adapter is connected to this socket.

2 Introduction
3 Host Communications Port (USB)

The host communications port connects the Agilent SPS 4 to a
computer controlling your Agilent instrument or in some cases, to the
instrument directly, using the furnished standard USB Type A to B
cable. The Agilent SPS 4 will appear as a virtual COM port under
Windows.
4 Auxiliary Communications Port (AUX)
The auxiliary communications port provides an RS485 interface, but is
not used in current Agilent software.
5 3- channel Peristaltic Pump
The peristaltic pump is located in the center on the rear panel.
Maximum two channels of three- channel pump are used to pump the
rinse solution to the fixed wash reservoir. One channel can also be used
to pump the used rinse solution to the waste container (optional). This
peristaltic pump may also be called a wash pump.
The wash pump speed is adjustable using the instrument control
software.
6 Purge Input
The purge input port is not currently used.
7 Cable Ducting
The cable ducting is used to hold the USB cable and the power cable.
8 Wash Pump Tubing Ducting
A ducting plate that extends vertically down the back of the
autosampler for routing the tubing from the wash pump to the fixed
wash reservoir. Clips are used to secure the wash pump tubing in place.
The same clips are also used to anchor the sample line from the sample
probe to an optimal length.

Introduction 2
Intended Use
The Agilent SPS 4 is designed to automate the presentation of liquid
samples, drawn from tubes held in racks loaded on the rack location mat,
to the instrument for analysis. The Agilent SPS 4 is controlled through
software commands sent via a USB interface.
The Agilent SPS 4 should only be used within the stated specifications.
If the equipment is used in a manner not specified by Agilent, the protection provided
CAUTION
by the equipment may be impaired.

2 Introduction
Specifications
Environmental
Your Agilent SPS 4 is designed for indoor use only. It is suitable for
Installation Category II and Pollution Degree 2. The site should be selected
to avoid dusty or corrosive atmospheres.
Table 1 Suitable environmental conditions for the Agilent SPS 4
Environmental Condition Specification
Temperature Range: Non-Operating -20°C–60°C, 20–80 % RH

-4°F–140°F, 20–80 % RH
Temperature Range: Operating 10°C–35°C, 20–80 % RH
50°F–95°F, 20–80 % RH
Operational Altitude 0–2000 m
0–6562 feet
Power
The power cable that is included with the Agilent SPS 4 has an AC
adapter. It is rated at the following.
Table 2 Input/output rating of the AC adapter for the Agilent SPS 4
Power Specification
Power Supply: Input: 100–240 V, 47/63 Hz, 1.5 A.

Output: 24 VDC, 2.5 A
Autosampler Input Power: Input: 24 VDC, 2.5 A

Introduction 2
Weights and Dimensions

Danger to hands and feet. When packaged the Agilent SPS 4 weighs approximately
WA R N I N G
18.6 kg (41.0 lbs). To avoid injury to personnel or damage to equipment, always use a
fork lift or other suitable lifting device when moving the instrument. If a fork lift is
not available, the Agilent SPS 4 will need to be lifted by two people.
Table 3 Weights and dimensions
Weights and Dimensions Specification
Length: 600 mm (23.6 in)
Depth: 363 mm (14.3 in)
Height: 510 mm (20.1 in)
Shipping Dimensions: * 812 mm x 532 mm x 714 mm,

(Packed in a Single Box) 32.0 in x 20.9 in x 28.1 in (approx.)
Net Weight * 15 kg, 33.1 lbs (approx.)
Shipping Weight: * Single Box 18.6 kg, 41.0 lbs (approx.)

*: For the mainframe only. Another package is added for accessory kits.
Key Specifications
Table 4 Agilent SPS 4 Key Specifications
Parameter Specification
Max. Number of Sample Racks 4
Max. Number of Standards racks 1
Max. Tube Height 150 mm (5.9 in)
Probe Wash Options Fixed Wash Reservoir (FWR) — single or optional dual
port
Wash Pump 3 individual channels, speed controlled by the instrument

control software

2 Introduction
Table 4 Agilent SPS 4 Key Specifications
Parameter Specification
Power/Error Indicators 4
Communications Connections Host: USB (5 VDC, 100 mA Max.)

Auxiliary: RS485 (-8–12.5 VDC, 250 mA Max.) [not used]
Host Interface Port USB (Type A to Type B)
Auxiliary Port (not used) RS485 (DB9 Male connector)

Introduction 2
Options and Accessories

The available options and accessories for the Agilent SPS 4 are listed in
Table 5.
Table 5 Available options and accessories
Description
Single port fixed wash reservoir kit
Standard cover kit
Sample probe kit, 0.25 mm ID
Dual port fixed wash reservoir
Microtiter plate adapter kit
High uptake volume rinse kit
Rinse tube kit for low volatile organic
Pump drain kit
Well plate startup kit
Nebulizer probe mount kit
Standard rack location mat
Sample probe to suit microtiter plate
Sample rack, round holes, 21 position, for 30 mm OD tubes, white

2 Introduction
Description
Standards rack, 5 wells x φ61 mm (suits LDPE 250 ml bottles)
Standards rack, 34 wells — 12 wells x φ29 mm and 22 wells x φ17 mm
Spill tray
Tube anchor
Peri pump tube 3 bridged 1.3 mm grey/grey 12/pk
Peri pump tube 3 bridged 2.29 mm purple/black 12/pk
Peri pump tube 3 bridged 2.79 mm purple/white 12/pk
Pump tubing Santoprene,3-bridged, 1.3 mm grey/grey, 12/pk
Polyethylene tube 1.5 mm x 0.6 mm
PTFE Tube 2 mm x 3 mm
Tubing, PVC, 8 mm x 5 mm, 2 m
Tubing, 2.5 mm ID, 2 m
Tubing-solvent resistant 0.093-in-ID, 2 m
Tubing-solvent resistant 0.187-in-ID, 2 m
Barb connector 2.5 mm-1.5 mm
Fitting-Barb straight-through 4.8 x 2.4 mm-ID-tubing polypropylene natural
Fitting-Barb straight-through 2.4 mm-ID-tubing polypropylene natural
LDPE Bottle 250 ml Narrow Mouth 250/pk
Polypropylene test tubes, 17 mm OD, 16.5 mL, 1000/pk
Centrifuge, screw capped, 30 mm OD, 50 mL, 500/pk polypropylene
Drainage Tank, polyethylene, 4 liter
Drainage Tank,10 liter
96-well Plates, 0.5 ml, PP 10pk

Introduction 2
Description
Rack overlay for 60 position sample rack

Allows 16 mm OD tubes to be used in 60 position rack (Qty 2 recommended per rack)
Rack overlay for 40 position sample rack

Allows 18 mm OD tubes to be used in 40 position rack (Qty 2 recommended per rack)

2 Introduction
This page is intentionally left blank.

User’s Guide
3
Installation
Installation Procedure 30
Orientation 30
Bench Requirements 32
Inspection and Unpacking 32
Fitting the Spill Tray 34
Installing the Rack Location Mat 36
Installing the Wash Reservoir 37
Installing the Standards Rack 39
Installing the Sample Racks 40
Fitting the Sample Probe 42
Fitting the Wash Pump Tubing 44
Connecting the Power and the Communications Cable 48
Confirming the Operation 50
Setting up the Instrument Controlling Software for SPS 4 50
Notes on Lifting the Autosampler 51
The Agilent SPS 4 autosampler is designed to be completely

self- installable. Instructions for setting up the autosampler
are included in this manual. For more information on setting
up the instrument software, refer to the documents provided
with the instrument or the instrument's help.
This chapter provides the installation instructions for the
SPS 4 unit and typical accessories. For other optional
accessories, refer to the installation instructions of the
accessories.
3 Installation
Installation Procedure
Carry out the assembly and test instructions as listed in the order below.
1 Inspection and Unpacking
2 Installing the Cover Kit
3 Fitting the Spill Tray
4 Installing the Rack Location Mat
5 Installing the Wash Reservoir
6 Installing the Standards Rack
7 Installing the Sample Racks
8 Fitting the Sample Probe
9 Fitting the Wash Pump Tubing
10 Connecting the Power and the Communications Cable
11 Confirming the Operation
12 Setting up the Instrument Controlling Software for SPS 4
Position the autosampler so that it is easy to access the mains power switch on the
WA R N I N G
wall outlet or to remove the power cord from the autosampler or wall outlet.
Orientation
Any references to “Front”, “Left”, “Right” or “Rear”, and 3 axes of
movement for the sample probe — longitudinal (X- axis), rotary or theta
(T- axis) and up and down (Z- axis) are as shown in Figure 3.

Installation 3
X-Axis
Front
T-Axis Z-Axis
Left
Right
Rear
Figure 3 Autosampler orientation

3 Installation
Bench Requirements
The autosampler should be placed on a bench with a height between
790 mm (31.1") and 910 mm (35.8") for optimal user ergonomics. A
footprint of 600 mm × 363 mm (23.6" × 14.3") is required. The bench
should be flat with no more than 3 mm (7/64”) variation. If a sampler
cover kit is fitted, additional space should be allowed on the side where
the exhaust hose ducting is located.
The autosampler should be located in close proximity to your instrument
so as to reduce the delay caused by the length of capillary tubing
connecting to the autosampler.
Inspection and Unpacking
Unpacking and Lifting

Keep the shipping box and packaging. If you need to return the autosampler during the
NOTE warranty period, it is a requirement of the warranty that the equipment is repacked in the
original shipping box. For instructions on packing the equipment, refer to the
Packing/Unpacking Guide.
Inspect all items for damage. Any damage or missing items should be reported to
CAUTION
Agilent immediately. Cite the part number and serial number of your autosampler.
Danger to hands and feet. When unpacked the autosampler is heavy. To avoid injury
WA R N I N G
to personnel or damage to equipment, always use at least two people to lift the
autosampler into position.

Installation 3
The unit should be lifted/moved from the positions shown in Figure 4.

One person should be at the front and one person should be at the rear of
the unit. Bend at the knees if lifting from a low position.
At least one Note: Remove

person lifting these plastic
from rear. inserts before
lifting unit.
At least one
person lifting
from front.
Figure 4 Lifting positions for removing the autosampler from shipping carton
Place the unit on a suitable bench and check all of the items you have
received against the packing list. A typical autosampler configuration
could include the following items:
• Spill tray
• Rack location mat
• Sample probe *
• Standards rack *
• Sample racks *
• Wash reservoir kit *
• Communications cable (USB) *
• Power supply cable including an AC adapter (as appropriate for your
location) **
• Tubing (including 3- bridge pump tubing) *
*: These items are packed in an accessory kit box.
**: This item is packed in another box.

3 Installation
Installing the Cover Kit
A Protective Cover is available as an optional accessory. It is used to

protect:
• samples from drafts, dust and airborne contamination
• the operating environment and/or the autosampler from the fumes or
solvent vapors arising from exposed liquids
• the operator from possible injury caused by the movement of the probe
arm or sharp edged sample probe
• the operator from sample fumes
It is made from transparent plastic panels provided in any easy- to- fit kit
form. When fitted, the protective cover creates a closed environment and
takes up no additional bench space. The autosampler working area is
accessed through a sliding door that can lock in a raised position to allow
for easy access to individual samples or racks.
When you install the autosampler with the optional Cover Kit, install the Cover Kit before
NOTE installing the autosampler. For instructions on installing the Cover Kit, refer to “Installing
the Cover Kit” on Page 82.
It is recommended that the autosampler is fitted with a Cover kit when handling
WA R N I N G
acidic solutions (samples, standards or rinse solutions), In this case, the fumes
must be extracted continuously and at all times through an exhaust hose, which is
connected to one of the Cover’s side panels.
Ensure that the autosampler does not have a cover kit installed if working with
organic solvents.
Fitting the Spill Tray

To prevent you and the instrument from earthquake damage, it is recommended to
WA R N I N G
secure the main body of the autosampler on your bench before this step. For how to
secure, see “Preventing Earthquake Damage” on Page 94.


Installation 3
Base bars
Spill tray
Front edge
Figure 5 Spill tray
The autosampler has a spill tray underneath the rack location mat to
contain any excess liquid that occurs as a result of an accidental spillage,
for example, from a dropped sample rack or maybe an unanticipated
overflow of the wash reservoir.
Place the spill tray flat on the deepest section located between the base
bars. It should be fitted with the front edge oriented as shown in
Figure 5.
It is recommended that any ingress of liquid into the spill tray is cleaned
up immediately after a spill has occurred. To access the spill tray for
cleaning, the sample racks, the standards rack and the fixed wash
reservoir (together with associated tubing) need to be removed from the
rack location mat (refer to “Installing the Rack Location Mat” on Page 36)
before it can be lifted off the spill tray to access any contained liquid.
If rinse fluid, sample fluid, standard fluid or other liquids spill on the spill tray, wipe
WA R N I N G
up immediately. These liquids may corrode the instruments. Highly alkaline or acidic
fluids can injure the skin and mucus membranes. Exercise caution when handling
these fluids.

3 Installation
Installing the Rack Location Mat
Default fixed wash

reservoir position
Dovetail feature
located at left edge
of frame
Figure 6 Rack location mat
The autosampler has a separate rack location mat which sits on top of the
spill tray and positions the sample racks (or a rack adapter for 96- well
microtiter plates), standards rack and fixed wash reservoir.
To fit the rack location mat:
1 Ensure that the spill tray is correctly fitted onto the autosampler base
bars.
2 One end of the rack location mat has a "dovetail" feature as shown in
Figures 6 and 7. Orient the rack location mat so the dovetail feature is
on the left hand side.
Figure 7 Rack location mat fitted to the left end frame
3 Ensure that the slot for the fixed wash reservoir is located at the rear
of the unit when installing the rack location mat.

Installation 3
4 Position the rack location mat over the spill tray and align the dovetail
feature in the mat with the dovetail feature in the left end frame before
pressing down firmly.
5 Check that the rack location mat fits firmly in place on the spill tray by
trying to move it from side to side and front to back. There should not
be any movement when fitted correctly.
6 If the rack location mat does not fit correctly even after completing the
steps above, contact your local Agilent office or representative.
Installing the Wash Reservoir
Figure 8 Single port fixed wash reservoir and kit
The fixed wash reservoir is supplied as a kit with the inlet and outlet
nipples fitted along with some lengths of inlet (2.5 mm ID) tubing and
outlet (5.0 mm ID) tubing. For the single port fixed wash reservoir, rinse
solution flows up through the smaller wash chamber and overflows into
the larger drain chamber to waste. For the dual port fixed wash reservoir,
there are two wash chambers and a common drain chamber.
The fixed wash reservoir is mounted at the rear of the rack location mat
(see Figure 6).
To install the fixed wash reservoir:
1 Put the base of the fixed wash reservoir into the locating hole and twist
it clockwise 60 degrees so it locks into position. The nipples on the
fixed wash reservoir should be facing toward the rear of the

3 Installation
autosampler (see Figure 8).

2 Ensure that the fixed wash reservoir is seated correctly by visually
checking that it is both perpendicular and firmly attached to the rack
location mat.
3 The larger (5 mm) nipple is the outlet nipple. Attach the 5 mm ID
tubing and place the other end of the tubing (drain line) into a suitable
waste container.
If a rear autosampler cover panel is fitted, the drain line needs to be
guided through the oval rubber grommet in the bottom of the rear cover
(see Figure 9).
Air locks in the drain line may cause rinse solution in the fixed wash reservoir to back up
NOTE and overflow. To prevent air locks, the drain line should fall freely and drop vertically into the
waste container. The end of the drain line should be suspended above the liquid level in the
waste container.
4 The 2.5 mm tubing is attached to the inlet (lower) nipple which, in

turn, is connected to the outlet tubing from the wash pump. For the
dual port fixed wash reservoir, there are two inlet nipples which will
need to be connected to tubing from 2 separate channels of the wash
pump.
If a rear autosampler cover panel is fitted, the inlet tubing from the
wash pump needs to be guided through the oval rubber grommet in the
bottom of the rear cover (see Figure 9).
5 Re- fit the rack location mat to the unit.
Figure 9 Feeding tubing through rubber grommet on rear cover (if fitted)

Installation 3
For the materials of the rinse solution, see “Default Rinse Materials” on
Page 95.
Check the following points regularly:

NOTE
• There is no leakage between the main body and the connector of the
fixed wash reservoir.
• The drain is not blocked by rinse and sample solution chemistry.
Installing the Standards Rack
Figure 10 Standards racks
The standards racks are located in the center of the rack location mat by
placing the rack’s feet into the holes in the rack location mat. There are 2
standard rack options as shown in Figure 10. Either may be supplied with
the equipment depending on your application.
• 34 well rack (12 wells for 29 mm OD tubes + 22 wells for 17 mm OD
tubes)
• 5 well rack (for 61 mm OD bottles)
To install the standards rack:
1 Locate the holes for the standards rack in the rack location mat.
2 Align the feet from the standards rack with the holes in the rack
location mat. Press down on the rack to ensure it is correctly located
and the bottom of the standards rack is sitting flush with the rack
location mat.

3 Installation
Figure 11 Installing the standards rack
Installing the Sample Racks

The sample racks are located on both (left and right) sides of the
standards rack by mating the rack’s feet with the holes in the rack
location mat. The rack location mat has maximum four sample rack
positions — two rack positions on the either side of the standards rack
position. The following rack options are supported.
To install the sample racks:
1 Locate the holes for the sample racks in the rack location mat.
2 Align the feet from each sample rack with the holes in the rack location
mat. Press down on the rack to ensure it is correctly located and the
bottom of the sample rack is sitting flush with the rack location mat.

Installation 3
To ensure the sample racks are seated correctly in the rack location mat, it is recommended
NOTE that you use the sample racks supplied with the autosampler. Sample racks from alternative
suppliers may not fit correctly.
Figure 12 Autosampler with 2 sample racks fitted either side of a standard rack
It is important to ensure that the vials sit vertically within the sample rack. If a vial sits
CAUTION
at an angle in the rack, the probe may hit the side of the vial as it enters or leaves the
vial. This can affect the alignment of the probe and/or damage the vial and the probe,
which might lead to loss of sample.
If there is a gap between the diameters of the vial and the rack well, the vials may not
sit vertically, resulting in the probe hitting the edge of a vial. Ensure that the selected
vials fit the rack correctly.
For example, a 60 position rack needs vials that OD is within 16.1 mm to 16.5 mm at a
height of 60 mm. If the diameter is less than or equal to 16.1 mm, you may be able to
use them with optional rack overlays.

3 Installation
Fitting the Sample Probe

Always switch off the power to the autosampler before installing or removing the
WA R N I N G
sample probe.
Tube restraint
Z-Axis Slide
Probe mount block
Mount nut
Eyelet for tube routing
Sample probe
Tube routing this side Accessories plate
Figure 13 Installing the sample probe
To fit the sample probe:

1 Turn the autosampler off.
2 Manually rotate the probe arm so it faces the front of the autosampler
for easy access.
3 Undo the mount nut from the probe mount block on the sample probe
and slide the nut off the sample probe.
4 Place the mount nut from the sample probe on top of the accessories
plate with the recessed edge facing up and the holes approximately
aligned.
5 Move the Z- axis slide down stopping just above mount nut sitting on
the accessories plate.

Installation 3
6 Slip the tip of the sample probe into the hole in the middle of the
Z- axis slide, through the mount nut and the accessories plate until the
probe mount block is sitting in the z- axis slide.
7 Slide the mount nut up onto the probe mount block and tighten to
secure the sample probe to the Z- axis slide.
8 Route the sample line from the sample probe to the left around the
probe arm housing. When using a carbon fiber sample probe, do not
run the sample line through the hook (or tube restraint) at the top of
the Z- axis slide. Connect the cable tie to the eyelet on the underside of
the probe arm cover and leave a loop of approximately 10 mm. Thread
the sample line through the loop in the cable tie as shown in Figure 14.
Do not use the tube restraint for pre- formed sample probes. It is only
NOTE used for non pre- formed sample probes.
Cable tie tubing through Tube Anchor Grommet

the eyelet on bottom of
housing
Colored Markers
Figure 14 Routing the sample line tubing

3 Installation
9 Feed the sample line through the rubber grommet in the middle of the
wash pump tubing ducting and then slot the sample line into the tube
anchor clip between the colored markers on the tubing as shown in
Figure 14. The tube markers on the sample line define the optimal
sample length of sample line from the sample probe to the exit point of
the autosampler.
The following lists the color of the tube markers on the sample probe
tubes.
• Red: Sample probe kit, 0.25 mm ID
• Yellow: Sample probe kit, 0.5 mm ID
• Green: Sample probe kit, 0.8 mm ID
• Blue: Sample probe kit, 1.0 mm ID
• Black: Sample probe kit, 1.2 mm ID
• Red: Sample probe to suit microtiter plate
10 Connect the sample line to the input of your instrument's sample
introduction system, adjusting the length from the exit point as
required.
It is important to ensure that the length of the tubing allows the probe arm to move
CAUTION
freely in all axes. If it is too tight, it will restrict the probe arm and may cause a
movement failure. If it is too loose, the probe arm may become tangled in the tubing as
it moves.
Fitting the Wash Pump Tubing

A wash pump is fitted on the rear of the autosampler and is used to
supply fresh rinse solution to the fixed wash reservoir. The wash pump is
a three channel peristaltic pump with four rollers and an individual
pressure plate for each channel. One and two channels of the wash pump
are used to feed rinse solution into the single and dual port fixed wash
reservoirs respectively. The third channel can also be used to pump the
used rinse solution to the waste container (optional).
The wash pump is controlled by the instrument control software and
rotates clockwise.

Installation 3
The wash pump accommodates standard 3 bridged pump tubing which is

available in a range of internal diameters for delivering different flow
rates. The optimal distance between the bridges on the pump tubing is
82–85 mm.
Wash pump tubing is available in different tube materials so ensure that you select a tube
NOTE material that is compatible with the rinse solution. If you are not sure which material to use,
contact your Agilent representative for a recommendation.
When a pump channel is not in use, release the occlusion arm on the channel and remove
the tubing from the guides to extend the life of the tubing.
Occlusion arm and spring
Pressure plate
Rotor Tube clamping block
Pump tubing
Tube anchor clip
Figure 15 Wash pump tube routing and connections
To get access to the wash pump to fit or change the pump tubing, the
autosampler may need to be moved.
Before moving the autosampler, ensure all sample racks and the standards rack have been
NOTE removed. In addition it may be necessary to disconnect the power cable, interface cable,
sample line, inlet line to the wash pump or the drain line from the fixed wash reservoir
depending on the distance moved.

3 Installation
Two Drain Arrangements

The waste rinse solution drains from the wash reservoir by means of a
gravity drain, which is the standard arrangement for draining the wash
reservoir. If preferred, you can create a pumped drain arrangement. This
section introduces the gravity drain arrangement and the pumped drain
arrangement.
Gravity Drain Arrangement

In a gravity drain arrangement, the rinse solution moves through a
channel (and two channels for dual port wash reservoir) of the peristaltic
pump to the inlet of the wash reservoir, as shown in Figure 16. It then
drains out from the outlet of the wash reservoir into the waste container
by means of a gravity drain.
(A) For Single Port Wash Reservoir (B) For Dual Port Wash Reservoir
Single Port Wash Pump Dual Port Wash Pump
Wash Reservoir Wash Reservoir
OUT
OUT
FWD FWD FWD

IN
ROTATION ROTATION ROTATION

IN
OUT
OUT
OUT
IN
IN
IN
Flow
Flow
Waste Out Rinse Solution In Waste Out

Rinse Solution In
Figure 16 Gravity Drain Arrangement
Pumped Drain Arrangement

In a pumped drain arrangement, the rinse solution moves through a
channel (or two channels for dual port wash reservoir) of the peristaltic
pump to the inlet of the wash reservoir. It then drains out from the outlet
of the wash reservoir into the waste container through other channel of

Installation 3
the peristaltic pump, as shown in Figure 17.
Wash Reservoir Wash Pump
OUT
FWD
IN
ROTATION
OUT
IN
Flow
Waste Out
Figure 17 Pumped Drain Arrangement
Loading Wash Pump Tubing

1 Release the pressure plate by disengaging the occlusion arm.
2 Fit one of the outside bridges on the pump tubing into the appropriate
left tube clamping block.
3 Feed the tubing around the rotor and fit the center tube bridge into the
right tube clamping block. Ensure there are no twists or kinks in the
tubing after loading as this will adversely affect tube life.
4 Reposition the pressure plate over the rotor and fix by slotting the
occlusion arm back into the track slot.
5 Route the outlet of the wash pump tubing through the tube anchor clips
to the delivery line or input of the fixed wash reservoir.
6 Route the inlet line of the wash pump tubing through the tube anchor
clip(s) and connect this to the suction line from the rinse solution
reservoir.

3 Installation
• Keep delivery and suction lines as short as possible.

NOTE
• Run at a slow speed when pumping viscous fluids.
• Use the largest bore tube running at a slow speed for the longest tube life.
• It is recommended to release the occlusion arm when the pump is not in use to extend
the life of the pump tubing.
Pressure Plate Adjustment

The position of the occlusion spring on each channel of the wash pump is
set in the factory to optimize the tension on the pressure plate when
pump tubing is fitted to the wash pump. It is possible to adjust the
tension on the pressure plate on a specific channel (only if the wash
pump can be turned on via the instrument control software) using the
following procedure:
1 Fit a new pump tube to the specific wash pump channel as described
on Page 47.
2 Connect the inlet line to the wash reservoir and place the outlet line
into a beaker.
3 Loosen the occlusion screw with a 5.5 mm (7/32”) wrench until there is
no pressure on the pump tubing.
4 Turn the wash pump on using the instrument control software.
5 While the wash pump is running, tighten the occlusion screw until rinse
solution starts to flow.
6 Tighten the screw a further half turn.
Connecting the Power and the Communications Cable

Before connecting any part of the autosampler to the mains power supply,
check you have the correct power supply. The power requirements are
given in “Power” on Page 22.

Installation 3
Application of the wrong supply voltage can create a fire hazard, or a potentially
WA R N I N G
serious shock hazard, and can seriously damage the instrument and any attached
ancillary equipment. Do not connect the instrument to the mains power until you
have ensured that the mains power supply from the outlets in your laboratory is
within the range stated in “Power” on Page 22.
The rear panel of the autosampler contains the peristaltic pump, purge
input, the power socket and switch, and a USB port for communication
between the autosampler and the computer that is running instrument
control software, as shown in Figure 2 on Page 19.
To connect the power:
1 Ensure that the power switch is turned off.
2 Connect the barrel output connector of the AC adapter to the power
supply input on the rear panel of the autosampler.
3 Connect the input plug of the AC adapter to a suitable mains power
outlet.
Ensure the autosampler is always connected to the protective grounding of
the mains supply.
Do not switch on the autosampler until all checks and settings for the
operation have been completed.
Host Communications Port (USB)

The host communications port (USB) is used to connect the autosampler
to the computer controlling your instrument.
To connect your autosampler:
1 Plug one end of the supplied USB cable into the host communications
port.
2 Connect the other end of the USB cable to a USB port on your
computer or instrument.
3 Fix the USB cable and the power cable using the cable ducting on the
rear column of the autosampler.

3 Installation
Auxiliary Communications Port

NOTE
The Auxiliary communications port is not used in current Agilent software.
Confirming the Operation

When the autosampler is turned on it will go through initialization tests
and set the probe position. If the initialization process is not successful,
refer to the troubleshooting information that is provided in “Initialization
Problems” on Page 68.
Ensure that the probe arm’s movement is not interfered with during
operation/initialization.
During the initialization sequence:
1 The probe moves upwards on the Z- axis and stops.
2 The probe arm moves in the opposite X- axis direction to which the
probe arm is facing and homes against an end frame.
3 The X- axis may move to clear the end frame. The probe arm rotates
counter- clockwise on the Theta- axis and stops.
4 The X- axis homes against the left end frame.
5 The probe arm moves to the four corners of the rack location mat.
6 The probe arm stops above the fixed wash reservoir.
Setting up the Instrument Controlling Software for SPS 4

You need to set up your instrument control software in order to set
parameters such as the rack configuration, the communication port, probe
depth, and so on. For instructions on how to install your instrument
software, see the associated documentation or the instrument Help.
Ensure that the autosampler can be controlled by the instrument software
when setup is complete.

Installation 3
Notes on Lifting the Autosampler

When lifting the autosampler:
• Ensure the power is off and the power and USB cables are
disconnected.
• Remove any accessories fitted to the rack location mat such as sample
racks, standards rack, the fixed wash reservoir and any associated
tubing.
• Remove the clear side covers (if fitted).
• It is advisable to lift the autosampler with two people positioning their
hands through the end frames at either end as shown in Figure 18.
One person to lift One person to lift

with hands through with hands through
left window right window
Figure 18 Lifting positions for removing the autosampler
The autosampler should never be carried by the probe carriage or the wash pump
CAUTION
tubing ducting.

3 Installation

User’s Guide
4
Operation
Starting up the Autosampler 54
Shutting Down the Autosampler 55
Flushing the Wash Reservoir and Flow Path 55
Software Control 56
4 Operation
Starting up the Autosampler

Before starting the autosampler, carefully read Chapter 1, "Safety Practices
and Hazards" and ensure that the laboratory is set up according to the
details specified.
To start up the autosampler:
1 Ensure the autosampler is connected via a three- pin, grounded power
cord of the AC adapter to a three- pin, grounded power outlet.
2 Ensure that the wash reservoir is properly connected.
3 Turn on the power using the Power Switch on the rear panel of the
autosampler.
The autosampler performs an initialization sequence on startup. The
power/error indicator is illuminated green when the initialization
sequence is completed correctly.
The initialization sequence moves the probe arm through the four corners of the rack
NOTE location mat and finishes with the probe repositioned above the fixed wash reservoir. This
process takes about 30 seconds.
If an error occurs during the initialization sequence, refer to Chapter 6,

"Troubleshooting".
4 Adjust the pressure plates of the wash pump until the desired rinse
solution flow rate is achieved. Refer to “Pressure Plate Adjustment” on
Page 48.
5 Purge air from the wash system (wash reservoir and flow path) by
placing the rinse solution uptake tubing in the rinse solution source
and running the rinse solution through the wash reservoir.
Ensure there are no air bubbles visible in the rinse uptake tubing
before you start using the autosampler.

Operation 4
Shutting Down the Autosampler

To shutdown the autosampler, complete the following steps:
1 Drain the wash system by removing the rinse solution uptake tubing
from the rinse solution source. Let the wash pump run until all solution
drains from the tube attached to the wash reservoir outlet.
If you use a rinse solution other than deionized water, flush the wash
reservoir with deionized water before shutting down the autosampler.
For more information, see the following section, “Flushing the Wash
Reservoir and Flow Path” on Page 55.
2 Release the pressure plate on the wash pump.
Releasing the pressure plate decreases wear on the pump tubing.
3 Turn off the autosampler power switch.
Flushing the Wash Reservoir and Flow Path

Generally, you can operate the autosampler without flushing the wash
system (wash reservoir and flow path). Under normal circumstances, you
can simply drain the wash system prior to shutting down the autosampler.
However, you need to flush the wash reservoir and flow path under two
circumstances:
• during initial startup of the autosampler after installation
• following the use of strong bases, acids, or organic solvents as rinse
agents
Flushing the wash system during initial startup of the autosampler
removes any contaminants that could cause interference during sample
analysis. Flushing the wash system after using strong rinse agents
prevents degradation and failure of the flow path components.
To flush the wash reservoir and flow path, complete the following steps:
1 Insert the rinse uptake tubing into a deionized water source.
If you are flushing the wash system during initial startup, first use a 2% nitric acid solution
NOTE as the rinse agent, followed by deionized water.

4 Operation
2 Run the rinse solution through the wash reservoir and flow path for 5
to 10 minutes.
Once the wash system has been flushed, you can proceed with the
sampling sequence or drain the wash system as part of the shutdown
procedure.
Software Control
All operations of the autosampler can be controlled by commands from
the instrument control software. For details on how the software interface
interacts with the autosampler, consult the relevant section of the manuals
or instrument Help.

User’s Guide
5
Maintenance
Routine Maintenance 58
Cleaning 58
Replacing the Wash Pump Tubing 59
Lubrication 60
Service 60
Spare Parts 60
The Agilent SPS 4 autosampler has been designed for low

maintenance and there are no user adjustments necessary
under normal operating conditions.
5 Maintenance
Routine Maintenance
The following parts of the instrument require routine maintenance.
Daily
• Confirm that the capillary tubing from the probe to the nebulizer is
secure and kink free.
• Rinse the probe and transfer tubing by pumping rinse solution or
distilled water at the end of analysis.
• Clean up any spills and wipe the rack location mat and the probe arm
with a damp cloth. Refer to “Cleaning” on Page 58 for further details.
• Check the probe and capillary tubing for blockages.
Weekly
• Check the wash pump flow rates, and replace the pump tubing if
necessary. Alternatively, move the tubing so that the other set of
bridges are secured in the peristaltic pump.
Cleaning
The probe arm of the autosampler can move without warning that may pose a
WA R N I N G
potential hazard due to its moving parts. Always switch off the power to the
autosampler before cleaning the autosampler.
Clean any sample spills immediately to preserve the life of the instrument.
CAUTION
The exterior surfaces of the autosampler, spill tray, rack location mat and
accessories should be kept clean. All cleaning should be done with a soft,
lint- free cloth. If necessary, this cloth can be dampened with water or a
mild detergent.

Maintenance 5
Do not use organic solvents or abrasive cleaning agents. This will help to preserve the
CAUTION
plastic components and painted surfaces.
If necessary, the probe can be removed from the autosampler and placed
in an ultrasonic bath for cleaning.
A rinse solution should always be pumped through the autosampler at the end of a run.
NOTE
Replacing the Wash Pump Tubing

Routine maintenance of the autosampler includes replacement of the wash
pump tubing. Because of the operating nature of the wash pump, the
tubing will probably be the most frequently replaced item on the Agilent
SPS 4 autosampler. If you use strong bases, acids, or solvents as rinsing
agents, the tubing may break down rapidly.
The optimal distance between the bridges on the pump tubing is
82–85 mm.
To replace the wash pump tubing, complete the following steps:
1 Shutdown and unplug the autosampler.
2 Release the pressure plate by disengaging the occlusion arm.
3 Remove the old tubing, and replace the pump tubing.
Carefully pull or cut the old tubing to remove it.
Replace the new tubing carefully. Damage can result if you apply too
much force.
4 Install new tubing following the instructions in “Loading Wash Pump
Tubing” on Page 47.
• Keep delivery and suction lines as short as possible.

NOTE
• Run at a slow speed when pumping viscous fluids.
• Use the largest bore tube running at a slow speed for the longest tube life.
• It is recommended to release the occlusion arm when the pump is not in use to extend
the life of the pump tubing.

5 Maintenance
It is possible to adjust the tension on the pressure plate on a specific

channel (only if the wash pump can be turned on via the instrument
control software) using the following procedure. If necessary, adjust the
tension on the pressure plates following the instructions in “Pressure Plate
Adjustment” on Page 48.
Lubrication
The autosampler is self lubricating and does not need the user to apply
lubrication.
Service
An authorized Agilent customer support representative must perform all
servicing of the autosampler. For contact information, refer to the Agilent
Technologies website for your local representative’s contact details:
www.agilent.com
Spare Parts
For ordering information of the consumable spare parts, see the Agilent
website at www.agilent.com.
Only use Agilent spare parts with your autosampler.

NOTE
Table 6 Consumable spare parts
Description
Standard rack location mat
Spill tray
Tube anchor
Cable ducting

Maintenance 5
Table 6 Consumable spare parts
Description
Nipple, PVDF, 5 mm ID, FWR
Nipple, PVDF, 2.5 mm ID, FWR
Tubing, PVC, 8 mm OD x 5 mm ID
Tubing, 2.5 mm ID 2 meter length
Barb connector 2.5 mm - 1.5 mm

5 Maintenance

User’s Guide
6
Troubleshooting
Troubleshooting Guide 64
Power Supply Problems 65
Operating State Problems 65
Error Indicator Codes 66
Initialization Problems 68
Probing Problems 69
Communication Problems 70
If your Agilent SPS 4 Autosampler is not operating correctly,

it can be difficult to determine whether the condition is
caused by the autosampler, the instrument control software
or communication between the two.
This chapter provides some useful steps to diagnose most
problems related to the autosampler.
Never remove the top panel of the autosampler chassis.

WA R N I N G
6 Troubleshooting
Troubleshooting Guide
The Agilent SPS 4 is equipped with diagnostic tools such as power/error
indicator and initialization sequence on startup to assist in identifying the
problems.
Power/Error
Indicator: Provides useful information on the error state of the
autosampler.
Initialization
Sequence: Tests that the probe arm functions in all its movement
axes by moving through the standard and sample
positions for the currently loaded mat and rack settings.
To identify your problem:
• If the autosampler appears to have no power and the power/error
indicator is not illuminated, refer to “Power Supply Problems” on
Page 65.
• If the power/error indicator is not lit or the LEDs are lit RED, refer to
“Operating State Problems” on Page 65. For the error state indicated by
the power/error indicator, refer to “Error Indicator Codes” on Page 66.
• If the autosampler fails in the initialization sequence, refer to
“Initialization Problems” on Page 68.
• If the initialization sequence is successful but the autosampler has a
malfunction in the probing operation, refer to “Probing Problems” on
Page 69.
• If the initialization sequence is successful but you cannot control the
autosampler from the instrument control software, refer to
“Communication Problems” on Page 70.
If these diagnostic tools do not assist you in identifying your problem,
contact an authorized Agilent representative. Refer to the Agilent
Technologies website for your local representative’s contact details:
www.agilent.com

Troubleshooting 6
Power Supply Problems

If the Agilent SPS 4 appears to have no power and the power/error
indicator is not illuminated:
1 Check that the power cable is connected to the power supply and that
it is turned on.
2 Visually check the Agilent SPS 4 and power lead for any electrical
damage.
3 If the Agilent SPS 4 still fails to initialize contact your Agilent customer
support representative.
Operating State Problems

The power/error indicator is used to identify the operating state of the
Agilent SPS 4:
• If it is lit GREEN then the Autosampler has power. It is reasonable to
assume that the autosampler is working correctly.
• If it is not lit, then turn off the power at the power switch on the rear
panel of the autosampler. Turn the power off at the power point. Check
that the power cable is correctly seated into the power supply. Check
the power plug from the power supply is inserted correctly into the
power supply inlet on the rear panel of the autosampler. Turn the
power on at the power point. Turn the power to the autosampler back
on at the power switch.
• If the autosampler initializes but the power/error indicator does not
illuminate then the indicator is potentially faulty. Contact your
authorized Agilent representative.
• If the autosampler does not initialize and the power/error indicator
does not illuminate then it is possible that either the power supply
or main board may be faulty. Contact your authorized Agilent
representative.
• If the autosampler does not complete initialization and the LEDs in
the power/error indicator are illuminated RED, the autosampler is in
an error state. Refer to the error codes in “Error Indicator Codes” on
Page 66 to identify the cause.

6 Troubleshooting
If the problem affecting operation cannot be identified, contact your

authorized Agilent representative.
Error Indicator Codes

The power/error indicator on the front panel is a line of four LEDs. These
LEDs illuminate RED when an error has occurred with the Agilent SPS 4.
The red illumination of these LEDs indicates a binary error code to assist
you in identifying the possible cause of the incorrect functioning of the
unit. Other more extensive error reporting is available via the software
commands.
Table 7 shows the binary error codes by the power/error indicator and
error descriptions. In the table, E1 means the leftmost LED, E4 means the
rightmost LED, 0 means LED off, G means LED (green) on, and R means
LED (red) on.
If an error occurs, a combination of the LEDs show the binary error code
for the fault and an error message is sent to the instrument control
software. For example, Error 7 = 0RRR is a theta- axis position error.
Table 7 Error Indicator Codes
E1 E2 E3 E4 Error Error Description Action

Code
G G G G 0 No error —
0 0 0 R 1 Main PCB Error Reinitialize the SPS 4. If
error persists, contact
your authorized Agilent
representative.
0 0 R 0 2 Program memory check-sum error Reinitialize the SPS 4. If

representative.
0 0 R R 3 Configuration memory check-sum error Reinitialize the SPS 4. If
representative.

Troubleshooting 6

Code
0 R 0 0 4 RAM test error Reinitialize the SPS 4. If
representative.
0 R 0 R 5 Axis initialization error Reinitialize the SPS 4. If

representative.
0 R R 0 6 X-axis position error. The X-axis motor Refer to “Probing

was commanded to move, but did not Problems” on Page 69
reach the desired position. for details.
0 R R R 7 Theta-axis position error. The Refer to “Probing

Theta-axis motor was commanded to Problems” on Page 69
move, but did not reach the desired for details.
position.
R 0 0 0 8 Z-axis position error. The Z-axis motor Refer to “Probing

was commanded to move, but did not Problems” on Page 69
reach the desired position. for details.
R 0 0 R 9 EEPROM error Reinitialize the SPS 4. If

representative.
R 0 R 0 10 Mat checksum error Reinitialize the SPS 4. If

representative.
R 0 R R 11 X-axis movement detected without the Refer to “Probing

motor being commanded to move. Problems” on Page 69
for details.
R R 0 0 12 Theta-axis movement detected without Refer to “Probing

the motor being commanded to move. Problems” on Page 69
for details.

6 Troubleshooting

Code
R R 0 R 13 Z-axis movement detected without the Refer to “Probing
motor being commanded to move. Problems” on Page 69
for details.
R R R 0 14 Reserved. —
R R R R 15 CPU exception error Reinitialize the SPS 4. If
representative.
Initialization Problems
The initialization sequence is a powerful self- diagnostic tool. It runs
immediately after the autosampler is powered on and tests the
functionality of the autosampler. The probe arm goes through a sequence
of movements and stops above the fixed wash reservoir position on the
rack location mat. If there is a problem with the autosampler, then it will
likely manifest itself at some stage during the initialization sequence.
The correct initialization sequence can be seen on Page 50.
If the autosampler fails to complete initialization, the power/error
indicator illuminates to display the error condition. See Table 7 for the
error indicator codes.
Check the Agilent SPS 4 by doing the following:
1 Turn the power off and move each axis manually until they stop and
determine if there is any mechanical obstruction preventing full travel.
2 If no mechanical obstruction can be identified turn the unit back on
and allow it to attempt the initialization process again.
3 If unsuccessful, contact your Agilent customer service representative.

Troubleshooting 6
Probing Problems
Probe Arm Jam

A probe arm jam is indicated when the Agilent SPS 4 cannot complete its
instructed move. This could be caused by:
• The probe arm hitting an obstacle or the sample line is restricted while
moving into position.
• The moving components have excessive friction.
• The position of the probe arm has been moved without being driven by
the autosampler (for example, when bumped by a user).
If this happens, check the autosampler by doing the following:
1 Turn the power off and move each axis manually until they stop and
determine if there is any mechanical obstruction preventing full travel.
2 If no mechanical obstruction can be identified turn the unit back on
and allow it to attempt the initialization sequence again.
3 If unsuccessful contact your Agilent customer support representative.
When the Sample Probe Hits an Object

If the sample probe hits an object like a sample rack or sample vial, or it
misses the wash position in the fixed wash reservoir, it may generate
error condition 13 (refer to Table 1) and the autosampler will stop. Check
the following:
1 Check that the probe is straight and mounted tightly to the Z- axis slide.
2 The probe should pass through the center of the accessories plate at
the bottom of the probe arm (see Figure 13).
3 Check that the spill tray and rack location mat are fitted correctly (see
Chapter 3, "Installation" for instructions).
4 Check that sample tubes sit vertically in the sample rack and are not
leaning to one side. If the sample tubes are not suitable for use with
the sample rack, change them to the correct diameter tubes to suit the
sample rack or change to the correct sample rack size.

6 Troubleshooting
5 Check that the fixed wash reservoir is correctly mounted.

6 Check that the rack configuration is correct.
If all accessories are fitted correctly, reset the unit and attempt to enter
the fixed wash reservoir again. If unsuccessful, perform user calibration.
User calibration makes minor adjustments to the centering of the sample
probe on the Agilent SPS 4.
For information on user calibration, refer to Chapter 7, "Running the
Calibration Wizard".
If you performed the calibration procedure but the problem remains
unresolved, contact your Agilent customer support representative.
Communication Problems
1 Check that the computer and the autosampler have the same
communication settings.
2 Disconnect the USB cable and initialize the autosampler to ensure that
it is in a working state. To do this, turn off the power switch, wait
10 seconds and then turn the power switch on. If the Agilent SPS 4
completes the initialization sequence with no error indicators, then this
usually indicates that the autosampler is working fine.
3 Reconnect the USB cable and ensure it is firmly seated at both ends.
4 Using the instrument control software, send a command to the Agilent
SPS 4. If the Agilent SPS 4 responds when the command is sent, this
means it has received the command from the computer. If unsuccessful,
contact your authorized Agilent representative for further instructions.

User’s Guide
7
Running the Calibration Wizard
Before User Calibration 72
Calibration Wizard Overview 72
Performing User Calibration 74
Error List 79
The sample probe can be displaced from the center after

hitting a sample vial or the sides of the fixed wash reservoir.
User calibration allows for minor adjustments to center the
sample probe. This chapter provides instructions on
performing user calibration for the Agilent SPS 4
autosampler using the Calibration Wizard software.
If centering for the sample probe on your autosampler is
misaligned, refer to Chapter 6, "Troubleshooting".
Before User Calibration

Check the following points before performing user calibration. Performing
user calibration when the unit and accessories have not been setup
correctly could cause a hazard.
• The spill tray is correctly fitted.
• The rack location mat is seated correctly in the spill tray and end
frames.
• The sample racks are correctly engaged in the locating holes in the rack
location mat and are seated flat on the rack location mat.
• A sample probe is fitted and has been mounted correctly.
• The sample probe is essentially straight.
Calibration Wizard Overview

Use the calibration wizard to adjust the centering of the sample probe.
There are four calibration points, one for each corner in the movement
range of the probe arm. At each calibration point, adjust the probe
position.
The following shows the Calibration Wizard window.
Figure 19 Calibration Wizard Window

Running the Calibration Wizard 7
Com Port: This area is used to connect this wizard to your autosampler. When
the wizard starts, select the relevant COM port, and then click
Connect. Refer to the instrument Help for more information.
Undo button: Reverts to the original calibration values and restarts the calibration
process.
Reset button: Starts the calibration process.
Mat Layout: The area under the Com Port area shows the information on the rack
layout specific to your autosampler when the calibration wizard has
successfully connected to your autosampler.
Z Axis Position: This area is used to adjust the Z-axis position of the sample probe at
each calibration point. The indicator shows the present height of
the sample probe. The Up/Down bottoms move the sample probe
height.
Probe Arm: This area is used to adjust the X and Y-axes positions of the sample
probe at each calibration point.
“Rear Left”, “Rear Right”, “Front Left”, and “Front Right”
buttons: Moves the probe to the calibration point “Rear Left”,
“Rear Right”, “Front Left”, or “Front Right”. Since the general
calibration process automatically moves the probe in the correct
order, you do not need to use these buttons. If you want to try again
after performing general calibration, we recommend moving the
probe to the calibration point you want to recalibrate.
The indicators next to the “Rear Left”, “Rear Right”, “Front Left”,
and “Front Right” buttons indicate the following status.
Orange: Ready to calibrate at this point.
Green: Calibration successful at this point.
Red: Not ready or calibration error.
Arrow buttons: Adjusts the XY position. Use the single arrow
buttons to move the sample probe 0.1 mm for fine adjustments and
the double arrow buttons to move it 1 mm for larger adjustments.
Capture button: Stores the calibration data at each calibration
point. Press this when the probe is centered in a good position in the
sample.

Performing User Calibration

To perform user calibration:
1 Ensure the autosampler power is on.
The sample racks fitted should be empty and not contain any sample
vials. If a microtiter plate Adapter is fitted, it should be loaded with
four 96 well microtiter plates.
Close the instrument software before launching the Calibration Wizard.

NOTE
2 Start the Calibration Wizard software. The Calibration Wizard window

opens as shown below.
3 Select the appropriate COM port from the drop- down list, and then
click Connect in the Com Port field.
If you chose the wrong one, an error message appears. If the
appropriate COM port number is not listed, check the autosampler
power, the USB connection, and the COM port setting in the instrument
control software.
4 If the calibration wizard can connect to your autosampler successfully,
the field under the Com Port field shows the information on the rack
layout specific to your autosampler. Ensure that the sample rack types
displayed on the screen correspond to those loaded into their respective
positions on the rack location mat.

5 Click Reset to start the calibration process.
During the calibration process, it is important that you do not click the Capture button while
NOTE moving to the different calibration points. If you have adjusted the position to be too far
away from the theoretical XY position at any point during the calibration, an error message
is displayed to instruct you to try again.
6 The button labeled “Rear Left” changes to orange and the sample probe
moves to point “Rear Left” above the far left sample well in the sample
rack or microtiter plate in that position. The sample probe is at the top
of the Z- Axis stroke (i.e., the Z- axis position is zero).
To adjust the probe position in point “Rear Left”:

a Adjust the sample probe height to the top of the sample well using
the Up/Down buttons.
b Adjust the XY position to the middle of the well using the arrow
buttons.
c When the position of the probe is centered in the sample well, click
Capture.
The indicator next to the “Rear Left” button changes from orange to
green.

7 When point “Rear Left” is captured, the sample probe moves to point
“Rear Right” above the far right sample well in the sample rack or
microtiter plate in that position, and the indicator next to the button
labeled “Rear Right” changes to orange.
To adjust the probe position in point “Rear Right”:

buttons.
Capture.
The indicator next to the “Rear Right” button changes from orange to
green.

8 When point “Rear Right” is captured, the sample probe moves to point
“Front Left” above the front left sample well in the sample rack or
labeled “Front Left” changes to orange.
To adjust the probe position in point “Front Left”:

buttons.
Capture.
The indicator next to the “Front Left” button changes from orange to
green.

9 When point “Front Left” is captured, the sample probe moves to point
“Front Right” above the front right sample well in the sample rack or
labeled “Front Right” changes to orange.
To adjust the probe position in point “Front Right”:

buttons.
Capture.
The indicator next to the “Front Right” button changes from orange
to green.

10 When all four points have been captured, a confirmation message is

displayed asking if you want to write the new calibration values.
• Click Yes to write the calibration values.

The autosampler is adjusted using the new calibration value.
• Click No if you want to retry.
The calibration process is restarted.
11 After calibration has been successfully completed, check the positioning
of the sample probe by clicking on each corner position button, and
driving the Z- Axis position down. Confirm that the sample probe is
positioned in the center of each well.
Error List
On running the calibration wizard software, you may encounter any error
listed below:
• Serial Port Error
Example: Autosampler did not respond.
A serial port error occurs if the software cannot communicate with the
autosampler. Check that the power is on, and no error lights are
illuminated. Also confirm the USB connection and the COM port setting
in the instrument control software. If the problem still remains
unresolved, contact your Agilent customer support representative.

• Envelope Error
Example: Probe reached the maximum Y limit.
An envelope error occurs if you try to move the sample probe outside
its motion envelope limits. If the well position is outside the motion
envelope, contact your Agilent customer support representative.
• Capture Failure
Example: Probe is too far from starting point.
A capture failure occurs if the point you are trying to capture is too far
away from the expected position. Check that the sample probe positions
above the correct well position, and the sample racks you are using
match the description in the Calibration Wizard. If the problem still
remains unresolved, contact your Agilent customer support
representative.
• Autosampler Error
Example: Move Z failed with ERR(8).
An autosampler error occurs if the arm has a collision, such as if the
probe hits the sample rack. If this happens, click the Reset button. The
autosampler will reset, and the calibration process can be continued. If
the problem still remains unresolved, contact your Agilent customer
support representative.
• Skipping Calibration
Example: None of the positions were adjusted. Skipping calibration.
If all four captured positions are within 0.5 mm of their original
position, the existing calibration is deemed to be accurate, so the
calibration does not be adjusted. In this case, any problems you are
having are unlikely to be caused by alignment. Contact your Agilent
customer support representative.

User’s Guide
A
Appendices
Preventing Earthquake Damage 94
Default Rinse Materials 95
A Appendices
Installing the Cover Kit
Introduction
The Autosampler Cover covers an Agilent SPS 4 autosampler and can be
used to:
• Help protect the operator and the laboratory environment from sample
vapors.
• Protect the samples from drafts, dust and possible airborne
contamination.
• Protect the autosampler from corrosive vapors arising from handling
acidic samples, standards or rinse solution.
Figure 20 Autosampler Cover
When fitted, the autosampler cover takes up no additional bench space.

The autosampler working area is accessed through a front sliding door
that can lock in a raised position to allow for easy access to individual
samples or racks (refer to Figure 20).

Appendices A
The autosampler cover requires approximately 10–20 minutes to assemble.

It is recommended that these instructions are read through first and then
followed step by step from the beginning.
Tools Required
The tools required are:
• Cotton or rubber gloves are recommended to avoid fingerprints on the
cover as a result of assembly.
Unpacking your Autosampler Cover

Use gloves to avoid fingerprints on the clear panels.
NOTE
Ensure you open the box from the top. Remove each component in its
individual wrapping and arrange these on a clean flat bench so they can
be recognized from the images in Figure 21.
Do not slide the clear panels across the bench or against each other to avoid
CAUTION
scratching.
The autosampler cover kit is supplied as an easy- to- fit kit and consists of
the following components:

A Appendices
1x Front Cover 1x Rear Cover 1x Side Cover with a hole 1x Side Cover (Blank)
1x Grommet
1x Duct Hose 1x Nut 1x Screw (fitted to rear cover)
Connection
Figure 21 Components in the autosampler cover kit
Installing your Autosampler Cover

Fitting the Rear Cover
1 Remove the cable ducting that clips onto the rear of the end frame to
hold the power and communication cables in place.
2 Remove the Spill Tray and the Rack Location Mat.
3 Lay the unit forward onto its front while supporting the probe arm.

Appendices A
4 Slide the Rear Cover into the slots in the bottom of the end frames
with the fitted grommet to the right side of the wash pump tubing
ducting as shown below.
Grommet in pump
ducting to align with
hole in cover
Grommet to right side
Remove cable ducting
5 Lift the top of the end cover up over the edge of the chassis as shown
below.
Lift cover up over

this edge

A Appendices
6 Align the hole in the wash pump tubing ducting with the hole in the
rear cover — a small amount of force may be applied as shown below.
Align hole in ducting

channel with hole in
cover
7 Fasten the Rear Cover to the wash pump tubing ducting using the
plastic screw and nut supplied as shown below. Tighten by hand only.
Fasten using screw

and nut

Appendices A
8 Re- fit the cable ducting to the right end frame. Route the power and
communication cables through the cable ducting and clip onto the right
end frame on the rear of the autosampler.
a Flare the bottom end of the cable ducting and fit to bottom of end
frame cover slot as shown below.
b Move along the ducting pushing into place so that it clips over the
outer edge of the end frame as shown below.

A Appendices
9 Stand the autosampler back up onto its feet and reposition. The rear
cover is now fitted as shown below.
10 Re- fit the Spill Tray.

11 Re- fit the Rack Location Mat, carefully feeding the fixed wash reservoir
tubing through the grommet in the rear cover.

Appendices A
Fitting the Side Covers

1 Fit the exhaust hose connection into the side cover with the hole by
rotating the end with the bayonet fitting clockwise as shown below.
2 Take the side cover with the exhaust hose connection fitted and fit to
the side of the autosampler that suits connection to an exhaust hose.
The side cover is pushed into the recesses of the end frame from the
inside and slides down to lock in place.
3 Fit the other side cover (blank) to the other end of the autosampler
using the same process.

A Appendices
Fitting the Front Cover

1 Position the front cover with the handle facing outwards and the
bottom fold facing inwards. Guide the front cover into the slots at the
top of the end frames as shown below.
2 Supporting the front cover underneath, gently guide the front cover
down until it sits on the rack location mat.

Appendices A
Door Operation
The door (front cover) has two positions:
1 In the Closed position, the door creates an enclosure around the
samples.
2 In the Open position, sample racks and standard racks/tubes can be
loaded or unloaded.
Dropping the front cover can cause liquid to splash from sample tubes which could
WA R N I N G
potentially be harmful to the operator. Gently slide the front cover down.
The door is held in the open position by a step in the end frame slots.
Do not remove samples racks or standard racks or vials while the autosampler is
WA R N I N G
operating.
Step in end frame slots

A Appendices
To change the sample probe or access the fixed wash reservoir, it is

recommended to first remove the front cover. Slide the front cover up
until the handle is almost touching the chassis and then flex it slightly
forward to remove.
Connections
General
There is an entry/exit point to the fixed wash reservoir through a rubber
grommet on the rear cover.
Exhaust Ducting
On the side cover there is a ducting connection for connecting exhaust
ducting. The hole will suit ducting with an internal diameter of 50 mm
(1.97”) used in conjunction with a cable tie or hose clamp.
Care and Maintenance

• Do not use abrasive cleaners or strong solvents to clean the cover as
this will reduce the clarity of the cover.
• Use a soft cloth and household window cleaner to clean off fingerprints
on the cover.
• If using volatile chemicals ensure the cover is adequately evacuated at
all times.
It is strongly recommended that an autosampler cover is fitted to the autosampler

WA R N I N G
when it is used with samples, standards or rinse solution(s) that release corrosive
fumes in any concentration. Whenever such liquids are present, the atmospheric
contents of the sample cover must be extracted continuously at a minimum rate of
350 liters per minute. Corrosive samples and standards should be removed from the
autosampler once analysis is completed.

Appendices A
Ensure that the autosampler DOES NOT have a cover kit installed if working with
WA R N I N G
organic solvents. Any failure in the extraction system could present a fire hazard.
Failure to provide adequate extraction at all times will potentially concentrate the
CAUTION
corrosive fumes and damage the autosampler. This damage is not covered by warranty.

A Appendices
Preventing Earthquake Damage

If necessary, prepare seismic brackets similar to those in figure below and
secure the brackets.
Seismic brackets are not supplied by Agilent. Prepare these items separately.
NOTE
8 mm
Bracket
(example)
188 mm
75 mm

Appendices A
Default Rinse Materials

To avoid damaging rinse lines, select materials with appropriate chemical
resistance for your application, particularly if you use a rinse solution
other than deionized water.
For your reference, the standard supplied rinse lines contain the following
materials:
Part Material
Polyethylene tube 1.5 mm x 0.6 mm Polyethylene

Peri pump tube Solva, 3 bridged grey/grey 12/pk Polyvinyl chloride (PVC)
Solvaflex (Solva)
Barb connector 2.5mm-1.5mm Polypropylene (PP)
Tubing, 2.5mm ID PVC
Nipple, PP, 2.5mm ID, FWR PP
Single port rinse station PP main part PP
Nipple, PVDF, 5mm ID, FWR Polyvinylidene fluoride
(PVDF)
Tubing, PVC, 8mm x 5mm PVC

A Appendices

www.agilent.com
In This Book
This book explains the
procedures necessary to use
the Agilent SPS 4
Autosampler to enhance
sample analysis.
© Agilent Technologies, Inc. 2015
Printed in Malaysia
Revision A, May 2015
*G8410-90000*
G8410-90000
Agilent Technologies

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Agilent SPS 4

Agilent SPS 4 Autosampler User’s Guide 3

Inspection and Unpacking 32

4 Agilent SPS 4 Autosampler User’s Guide

Replacing the Wash Pump Tubing 59

7 Running the Calibration Wizard

Agilent SPS 4 Autosampler User’s Guide 5

6 Agilent SPS 4 Autosampler User’s Guide

Your Agilent SPS 4 autosampler has been carefully designed

This equipment is rated Equipment Class I.

8 Agilent SPS 4 Autosampler User’s Guide

Agilent SPS 4 Autosampler User’s Guide 9

The CSA mark is a registered trademark of the Canadian Standards

10 Agilent SPS 4 Autosampler User’s Guide

The RCM mark is a registered trademark of the Australian

Agilent SPS 4 Autosampler User’s Guide 11

12 Agilent SPS 4 Autosampler User’s Guide

Agilent SPS 4 Autosampler User’s Guide 13

South Korean Class A EMC declaration

14 Agilent SPS 4 Autosampler User’s Guide

16 Agilent SPS 4 Autosampler User’s Guide

Front and Rear Views

Agilent SPS 4 Autosampler User’s Guide 17

18 Agilent SPS 4 Autosampler User’s Guide

Indicator Codes" on Page 66 for details of the various error codes.

Figure 2 Rear View of Agilent SPS 4

Agilent SPS 4 Autosampler User’s Guide 19

3 Host Communications Port (USB)

20 Agilent SPS 4 Autosampler User’s Guide

Agilent SPS 4 Autosampler User’s Guide 21

Table 1 Suitable environmental conditions for the Agilent SPS 4

Environmental Condition Specification

Temperature Range: Non-Operating -20°C–60°C, 20–80 % RH

Table 2 Input/output rating of the AC adapter for the Agilent SPS 4

Power Supply: Input: 100–240 V, 47/63 Hz, 1.5 A.

Autosampler Input Power: Input: 24 VDC, 2.5 A

22 Agilent SPS 4 Autosampler User’s Guide

Weights and Dimensions

Table 3 Weights and dimensions

Weights and Dimensions Specification

Length: 600 mm (23.6 in)

Depth: 363 mm (14.3 in)

Height: 510 mm (20.1 in)

Shipping Dimensions: * 812 mm x 532 mm x 714 mm,

Net Weight * 15 kg, 33.1 lbs (approx.)

Shipping Weight: * Single Box 18.6 kg, 41.0 lbs (approx.)

Max. Number of Sample Racks 4

Max. Number of Standards racks 1

Max. Tube Height 150 mm (5.9 in)

Wash Pump 3 individual channels, speed controlled by the instrument

Agilent SPS 4 Autosampler User’s Guide 23

Table 4 Agilent SPS 4 Key Specifications

Communications Connections Host: USB (5 VDC, 100 mA Max.)

Auxiliary Port (not used) RS485 (DB9 Male connector)

24 Agilent SPS 4 Autosampler User’s Guide

Options and Accessories

Table 5 Available options and accessories

Single port fixed wash reservoir kit

Standard cover kit

Sample probe kit, 0.25 mm ID

Sample probe kit, 0.5 mm ID

Sample probe kit, 0.8 mm ID

Sample probe kit, 1.0 mm ID

Sample probe kit, 1.2 mm ID