Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality Polyclonal
Host Rabbit
Size
Conjugate Unconjugated
0.025 mg
Order Details NB300-653SS
6-8 Business Days
Datasheet 0.1 mg
Reviews & Publications NB300-653
Protocols & FAQs
In Stock: 2-4 Business Days
Support & Research

Synaptophysin Antibody Summary

Synthetic peptide from the N-terminal region of human Synaptophysin,with

Immunogen sequence FATCGSYSGELQLSVDCANKTE, corresponding to amino
acids 41-62.
Localization Integral membrane protein. Synaptic vesicles.
Marker Pre-synaptic marker
The does not cross-react other synaptic vesicle proteins such as
Specificity
synaptotagmin, GAP45, and SNAP25 protiens.
Clonality Polyclonal
Host Rabbit
Species Human, Mouse, Rat, Chicken, Monkey
Gene SYP
Purity Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards
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a future purchase.
Reward

Applications/Dilutions
 Western Blot 1:500-1:1000
 Immunocytochemistry/Immunofluorescence
1:10-1:500
Dilutions  Immunohistochemistry 1:10-1:500
 Immunohistochemistry-Paraffin 1:10-1:500
 Immunoprecipitation 1:125
 Immunofluorescence (PMID: 21368026). Antibody
detects a 38 kDa band by WB.
Application Notes
Immunoprecipitation was reported in scientific
literature.
Positive Control

Brain Lysate (NB820-59705)

Pancreas Lysate (NB820-59713)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw
Storage
cycles.
Buffer Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
Preservative 0.02% Sodium Azide
Purity Immunogen affinity purified

Alternate Names for Synaptophysin Antibody

 Major synaptic vesicle protein p38
 MRX
 MRXSYP
 Synaptophysin
 SYP

Background
Synaptophysin is a 38 kDa synaptic vesicle associated protein. The pathology of synaptic
components is investigated by measuring the expression of synaptophysin that is mainly
concentrated in synaptic terminals. The expression of synaptophysin is a measure of dynamic
plastic changes in nerve tissue. A decrease in synaptophysin and other synaptic vesicle
associated proteins (Synaptotagmin, synaptosomal associated protein 25 kDa, SNAP-25, CLC
ad GAP-43) generally means degeneration in the nerve fibers and an increased expression
results in enhanced neuroplasticity and neuronal hypertrophy. In conditions involving
generative changes in the nerve tissue, the expression of psynaptophysin is generally reduced,
for example, in Schizophrenia an Alzhiemers disease. A decreased expression of synaptophysin
in hippocampus of Schizoprenic patients has also been reported.

Limitations
This product is for research use only and is not approved for use in humans or in clinical
diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Synaptophysin Antibody NB300-653SS
Species: Hu, Mu, Rt, Ch, Mk
Applications: WB, ICC/IF, IHC, IHC-P, IP

Mouse Brain Tissue

Human Cerebellum
Human Pancreas Tissue