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NIST-traceable pH buffer 45


To measure pH correctly, the electrode-instrument set Certificate of Analysis

needs to be calibrated regularly with buffer solutions. Each CRISON buffer solution is supplied with a
CRISON supplies three ready-to-use solutions. At 25°C, certificate of analysis that indicates:
their pH values are 4.01, 7.00 and 9.21. uncertainty, traceability, composition, batch number and ��
expiry date. (See Page 132)

pH 7,00 buffer: Potassium and di-sodium phosphates
pH 4.01 buffer: Potassium hydrogen phthalate
pH 9.21 buffer: Borax
They also contain germicide.

These are technical buffers prepared as per DIN 19267.
They are contrasted with standard reference material
(SRM) from the National Institute of Standards & Tech-
nology, NIST, in line with DIN 19266.
The pH tolerance range is ± 0.02 pH.
Buffer capacity: ß = 0.02 mol/l per pH unit.
Dilution value: Δ 1/2 ≤ 0.052 pH.
Shelf life: At least 2 years if stored correctly.
No colourings; they do not contaminate the electrode.
Immune to micro-organisms.
Economical. They can be used repeatedly, thanks to their
s an
excellent buffer capacity. commend f
CRISON re so lu ti o no
alkaline bu ec au se it
5 °C) b
pH 9.21 (2 th an solutions
ab le
is more st
pH values at different temperatures values.
with higher
ºC pH
0 4.01 7,12 9,52
10 4.00 7.06 9.38 Ordering information
20 4.00 7.02 9.26 Code Description
25 4.01 7.00 9.21 94 60 Buffer solution pH 4.01, 125 ml flask
30 4.01 6.99 9.16
94 61 Buffer solution pH 7.00, 125 ml flask
40 4.03 6.97 9.06
50 4.06 6.97 8.99 94 62 Buffer solution pH 9.21, 125 ml flask
60 4.10 6.98 8.93 94 63 Buffer solution pH 4.01, 250 ml flask
70 4.16 7.00 8.88
94 64 Buffer solution pH 7.00, 250 ml flask
80 4.22 7.04 8.83
90 4.30 7.09 8.79 94 65 Buffer solution pH 9.21, 250 ml flask

Laboratory Catalogue 2004

1st Edition
46 Electrolyte solutions and
electrode regenerators

Electrolyte solutions Regenerating solutions

An important part of the maintenance of liquid In some cases, these solutions can correct operating
electrolyte electrodes is to check the level of the anomalies in pH and redox electrodes.
�� reference electrolyte and refill when necessary. The most common symptoms of an electrode in need of
The reference electrolyte is also used to refill the regeneration are:
protective cap of the membrane when the electrode will • slow response.
not be used for a long time. • high asymmetry potential.
• poor sensitivity
CRISON supplies three electrode regenerating solutions.
This is a solution of KCl 3M. Electrode cleaner with pepsin
It is the electrolyte used with Solution of HCl 0.1 M, enriched with pepsin, pH ≈1.
reference element of encap- It is indicated in the regeneration of electrodes that
sulated AgCl crystals. usually measure samples with a high protein content,
such as milk, cheese, meat, blood, serum, etc...
CRISOLYT A The electrode is cleaned by removal of the proteins
This is a solution of KCl 3M, thanks to the hydrolysing ability of pepsin in acidic
saturated with AgCl. It is the mediums.
electrolyte used with referen- If used on electrodes with a polymer electrolyte, only
ce element of Ag/AgCl wire. the glass membrane should be immersed, never the
CRISOLYT-G, for specific applications
CRISOLYT-G is a KCI solution in which much of the Diaphragm cleaner
water is replaced by glycerine. The high glycerine Thiourea solution in HCl 0.1 M, pH ≈1.
content prevents a reaction between the KCI and the Appropriate for eliminating the black deposit which
proteins in the medium. This prevents early blockage forms on the ceramic diaphragm of the electrode,
of the diaphragm, extends the life of the electrode and blocking the electrolyte exit.
above all, ensures that the electrode operates soundly. The black diaphragm appears when an electrode
This electrolyte is recommended for measuring in: is used with a Ag/AgCI wire reference in samples
• samples with a high protein content. containing sulphides, reducing substances, etc... The sil-
• partially organic samples. ver salts which are formed are dissolved in the thiourea
• samples containing oils. solution.
• samples at low temperatures.
Membrane reactivator
This is a solution with 1-2 % HF.
CRISOLYT V It is recommended to reactivate pH electrodes with a
CRISOLYT V is a highly slow response or poor sensitivity. The hydrofluoric acid
viscous 3M KCI and tylose renews the hydrated gel layer of the membrane.
solution. This solution is useful when others have not had positive
This electrolyte is used in li- results, although success cannot be guaranteed.
quid electrolyte electrodes to If used on electrodes with a polymer electrolyte, only
reduce electrolyte flow and the glass membrane should be immersed, never the
hence the frequency with diaphragm.
which the electrolyte has to
be refilled. Ordering information
Code Description
KNO3 1M 95 00 CRISOLYT, KCI 3M, 250 ml flask
This is the reference elec-
95 01 CRISOLYT A, KCI 3M + AgCl, 250 ml flask
trolyte for metallic silver electrodes and some selective
electrodes. 95 02 CRISOLYT-G, KCI + glycerine, 250 ml flask
95 03 CRISOLYT V, KCl + tylose, 250 ml flask
LiCl 1 M in ethanol 95 20 LiCl 1M in ethanol, 250 ml flask
Reference electrolyte for titrations in non-aqueous 95 21 KNO3 1M, 250 ml flask
96 00 Electrode cleaner with pepsin, 250 ml flask
96 10 Diaphragm cleaner, 250 ml flask
96 20 Membrane reactivator, HF, 250 ml flask

Laboratory Catalogue 2004

1st Edition