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Lab Protocols > Methods > Electrophoresis > Gel Electrophoresis > Comparison of TAE and TBE electrophoresis
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APR 1 Comparison of TAE and TBE electrophoresis
2017
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Properties TBE TAE Remarks
repeated electrophoresis
stranded) DNA
Resolution High resolution High resolution for TBE supports agarose cross-linking better than
fragments fragments
puri⒄�ed DNA
Recovery of DNA Poor Good Borate from TBE bu er interacts with DNA
from agarose gel
Integrity of DNA High Low Borate from TBE bu er inhibits many enzyme
activity including DNA modifying enzymes
Cost Costly Cheaper Highly conc. TAE (50X) require less volume to
be transported.
DNA electrophoresis
http://www.scilifelab.com/techniques/comparisontaetbeelectrophoresisbuffer/ 1/2