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NET JRF Subjective Question Paper 1
Prepared for Biotecnika.org by
Prof Neha Suman
 This is the first set of 20 Subjective questions.
Solve them and send it in MS word format or PDF/
scanned format to info@biotecnika.org and we will
evaluate and let you know about your All India
score.

Instructions:
♣ Each question Carries 5 Marks.

♣ Reply each question in not less than 100 words

♣ Do not try to copy from Text Books since the

marks obtained by you will not reflect your

preparation level.
♣ It’s advisable that you first write the answers

first using pen and paper and later try to

compile it online.
1) Explain the role of following in gene cloning:
♣ Restriction endonucleases
♣ YAC
♣ Linkers
♣ adaptors
♣ Terminal nucleotidyl transferases
♣ Shuttle vectors
♣ Plasmids

2) What is southern hybridisation? Why it is so called? What is the basis of this

blotting technique? Make a list of the applications of this technique?

3) What do you understand by DNA sequencing? Kindly illustrate the two

methods of DNA sequencing?

4) Explain primer walking? How it is different from chromosome walking?

5) What is vector? Illustrate at least 5 properties of a good vector. What are the
differences between the cloning & expression vector? How are they designed?

6) How a target gene containing vector is transferred into the host? How a clone
containing a specific DNA insert can be selected?

7) What is the function of phenol-chloroform-isoamyl alcohol in DNA isolation?

Why phenol is used in DNA isolation despite the fact that it degrades the DNA?
How you can remove the excess of phenol in the isolated DNA?

8) What are DNA markers? Give a brief description of some important DNA
markers & discuss their application

9) what is the importance of agarose gel electrophoresis in RDT? How one can
visualise DNA incorporated in the gel? What is the significance of this process in
isolation & separation of DNA?

10) can DNA be chemically synthesized? How? What is the application of these
chemically synthesized DNA?

11) What is PCR? What si the principle behind this technique? Briefly describe
the process of PCR.

12) What do you understand by cDNA library? How it is different from genomic
DNA library? How the clones are stored using this library?

13) Suggest some of the screening strategies used for these libraries? Why it is
necessary to perform screening before storing the clones?
14) What is human genome project? How the genome was sequenced during
HGP? Whose genome was sequenced?
15) Apart from plasmids suggest some of the other vectors involved in RDT?
How are these vectors superior than the regular plasmid vectors? What type of
vectors we use in cDNA library?

16) What are DNA ligases? What is its function? What is the role of ligases in
RDT experiments? What are the cofactors needed for their action?

17) What is Ti plasmid? Where are they found? Draw a schematic diagram to
illustrate its structure

18) Suggest at least two vectors each involved in transferring DNA to plant &
animal hosts.

19) How the location of a gene can be studied? How much important is in situ
hybridization to visualize the position of a gene on a eukaryotic chromosome?

20) suggest different methods used in separation of DNA & RNA. Also explain
the principle behind these separation methods
 Thank You!!!
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