Rape investigation by using DNA TEST

Rape investigation is the procedure to gather facts about a suspected rape, including forensic
identification of a perpetrator, type of rape and other details.

The vast majority of rapes are committed by persons known to the victim: only five percent of assaults
are perpetrated by a stranger according to one survey. Therefore, the identity of the perpetrator is
frequently reported. Biological evidence such as semen, blood, vaginal secretions, saliva, vaginal
epithelial cells may be identified and genetically typed by a crime lab. The information derived from the
analysis can often help determine whether sexual contact occurred, provide information regarding the
circumstances of the incident, and be compared to reference samples collected from patients and
suspects. Personnel in the United States of America collect evidence for potential rape cases by using
rape kits. The time it takes to have rape kits processed has been criticized.

Perpetrator identification:

DNA profiling:

DNA profiling is used by crime laboratories for testing biological evidence, most commonly by means of
the polymerase chain reaction (PCR), which allows analysis of samples of limited quality and quantity by
making millions of copies. An advanced form of PCR testing called short tandem repeats (STR) generates
a DNA profile that can be compared to DNA from a suspect or a crime scene. Blood, buccal (inner cheek)
swabbings or saliva should also be collected from victims to distinguish their DNA from that of suspects.

Criminals may plant fake DNA samples at crime scenes. In one case Dr. John Schneeberger, who raped
one of his sedated patients and left semen on her underwear, surgically inserted a Penrose drain into his
arm and filled it with foreign blood and anticoagulants. Police drew what they believed to be
Schneeberger's blood and compared DNA on three occasions without a match.

Circumstances and type of rape:

Abrasions, bruises and lacerations on the victim help elucidate how a rape was carried out. 8 to 45
percent of victims show evidence of external trauma, most commonly at the mouth, throat, wrists,
arms, breasts and thighs: trauma to these sites comprise approximately two thirds of injuries, while
trauma to the vagina and perineum account for approximately 20 percent. Recent coitus can be
determined by performing a vaginal wet-mount microscopy examination (or oral/anal if indicated) for
detection of motile sperm, which are seen on the slide if less than three hours have elapsed since
ejaculation. However, only one-third of sexual assaults result in ejaculation into a body orifice. Further,
the alleged assailant may have had a vasectomy or have experienced sexual dysfunction (roughly 50
percent of assailants suffer from impotence or ejaculatory dysfunction). In addition, acid phosphatase
levels in high concentrations is a good indicator of recent coitus. Acid phosphatase is found in prostatic
secretions and activity decreases with time and is usually absent after 24 hours. Prostate-specific
antigen (PSA) may be detected within a 48-hour period. The seminal fluid of vasectomized men also
contains a significant PSA level. Nonmotile sperm may be detected even beyond 72 hours after
intercourse depending on staining techniques.

It was held by the Chandhigarh High Court [India] as reported in 2006 Volume No. [2] Name of Legal
Monthly Publication -Acquittal Page No. 199 – Chandhigarh High Court – Dilip Rao sahib Deshmukh, J. –
Bablu @ Uday Vs. State of Chandhigarh – Criminal Appeal No. 412 of 2006 – Decided on 27.7.2006 –
Where in a rape case doctor’s found hymen intact, merely because doctors stated that partial
penetration was possible, no such inference could be drawn in favor of the prosecution and the
conviction in the rape case was set aside by the High Court.