RESULT

Stock Diluted
Concentration of carmoisine Volume of Concentration Volume of
(ppm) carmoisine (ml) of carmoisine carmoisine (ml)
M1 V1 (ppm) V2
M2
1 100 2.5 5 50
2 100 7.5 15 50
3 100 12.5 25 50
4 100 17.5 35 50
5 100 22.5 45 50

Using formula:
M1V1 = M2V2
Machine: Shimadzu UV-1800
Wavelength scan
i. Instrument parameters
Starting wavelength = 400 nm
Ending wavelength = 800 nm
Path length =

ii. Result for wavelength scan

Sample used = carmoisine
Concentration of the sample =
ʎmax obtained = 517.0 nm
Absorbance = 1.816 A

Photometric scan
i. Instrument parameters
Wavelength, ʎ =
Path length =
ii. Results for photometric scan
No. Sample Concentration (ppm) Absorbance
1 Std 1 0 0.000
2 Std 2 5 0.190
 3 Std 3 15 0.560
4 Std 4 25 0.908
5 Std 5 35 1.197
6 Std 6 45 1.806
7 Unknown #1 50 0.772
8 Unknown #2 40 0.821

y = 0.0382x - 0.0192
R² = 0.9878
1.5

1
Absorbance

0.5

0
0 5 10 15 20 25 30 35 40 45 50

-0.5
concentration, ppm

R2 = 0.9878

Machine: Shimadzu UV-1800

Wavelength scan
iii. Instrument parameters
Starting wavelength = 400 nm
Ending wavelength = 800 nm
Path length =

iv. Result for wavelength scan

Sample used = carmoisine
Concentration of the sample =
ʎmax obtained = 516.0 nm
 Absorbance = 1.691 A

Photometric scan
iii. Instrument parameters
Wavelength, ʎ =
Path length =
iv. Results for photometric scan
No. Sample Concentration (ppm) Absorbance
1 Std 1 0 0.000
2 Std 2 5 0.187
3 Std 3 15 0.545
4 Std 4 25 0.900
5 Std 5 35 1.177
6 Std 6 45 1.710
7 Unknown #1 20 0.511
8 Unknown #2 80 1.471

1.8
y = 0.0366x - 0.0086
1.6 R² = 0.9929

1.4

1.2

1
Axis Title

0.8

0.6

0.4

0.2

0
0 5 10 15 20 25 30 35 40 45 50
-0.2
Axis Title

R2 = 0.9929