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Name: Enriquez, Vergel Jigs 4Bio8

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report

Pre-lab Quiz Results


You scored 100% by answering 4 out of 4 questions correctly.

1. Which of the following is true of osmosis?


You correctly answered: c. It is a type of diffusion.

2. Which of the following occurs when a hypertonic solution is added to cells?


You correctly answered: d. The cells shrink.

3. The variable that affects osmotic pressure is


You correctly answered: a. the concentration of nondiffusing solutes.

4. The net movement of water would be into the cell in a


You correctly answered: b. hypotonic solution.

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Experiment Results
Predict Question:
Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will have?
Your answer : a. increased osmotic pressure

Predict Question 2: What do you think will be the pressure result of the current experimental conditions?
Your answer : c. pressure above the left beaker

Stop & Think Questions:


Why do you think there is no pressure change?
You correctly answered: b. Sodium is able to diffuse through the pores.

Why do you think there was no pressure change?


You correctly answered: c. There is no net movement of water because the solute concentrations are the same.

Experiment Data:

Run Number Solute MWCO Start Conc. L Pres. L Start Conc. R Pres. R Rate
1 Na+ Cl- 20 5.00 170 0.00 0 0.0000
1 Albumin 20 0.00 170 0.00 0 0.0000
1 Glucose 20 0.00 170 0.00 0 0.0000
2 Na+ Cl- 20 10.00 340 0.00 0 0.0000
2 Albumin 20 0.00 340 0.00 0 0.0000
2 Glucose 20 0.00 340 0.00 0 0.0000
3 Na+ Cl- 50 10.00 0 0.00 0 0.0167
3 Albumin 50 0.00 0 0.00 0 0.0000
3 Glucose 50 0.00 0 0.00 0 0.0000
4 Na+ Cl- 100 0.00 136 0.00 0 0.0000
4 Albumin 100 0.00 136 0.00 0 0.0000
4 Glucose 100 8.00 136 0.00 0 0.0000
5 Na+ Cl- 200 0.00 0 0.00 0 0.0000
5 Albumin 200 0.00 0 0.00 0 0.0000
5 Glucose 200 8.00 0 0.00 0 0.0036
6 Na+ Cl- 200 0.00 153 0.00 0 0.0000
6 Albumin 200 9.00 153 0.00 0 0.0000
6 Glucose 200 0.00 153 10.00 0 0.0044

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Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic pressure will
You correctly answered: c. double.

2. Which membrane did not allow Na+ Cl- to pass through?


You correctly answered: a. 20 MWCO

3. When a solute is able to diffuse through a membrane


You correctly answered: b. equilibrium is reached.

4. Water diffuses
You correctly answered: c. toward solutes.

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Review Sheet Results
1. Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and why it has this effect. How well
did the results compare with your prediction?
Your answer:
The increase of NaCl resulted in an increase of osmotic pressure. I was right in predicting that the pressure would increase
due to the water diffusing from the lower concentration gradient to the higher concentration gradient but the some
membranes i.e. 20 MWCO membrance did not permit transport.

2. Describe one way in which osmosis is similar to simple diffusion and one way in which it is different.
Your answer:
Osmosis and diffusion both move from a higher concentration to a lower concentration. Osmosis differs from diffusion
because it only applies to water and goes in the direction of the solute.

3. Solutes are sometimes measured in milliosmoles. Explain the statement, “Water chases milliosmoles.”
Your answer:
It means that as a solute increases and the number of milliosmoles rise, the water will decrease.

4. The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO
membrane in place. Explain the results. How well did the results compare with your prediction?
Your answer:
The albumin was unable to diffuse through the membrane because it weighs too much. This resulted in a buildup of
pressure of 153 above the left beaker that contained the albium. The glucose diffused from right to left until equilibium was
reached.

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