BALCERA, GOROSPE, JAPLIT

MARTINEZG, SALINAS 4BIO5 (Mus Musculus) 23 NOV 2017

Gene Expression When Knocked-Out

NODAL § Expressed in the node § Unable to form

§ For formation of embryonic pattern
primitive streak and § Removal of one copy
node prevents formation of
§ required to form AVE multiple primitive
(anterior visceral streaks
endoderm)
§ mesoderm formation
§ left side; assoc with
left-right assymetry
formation

activin receptor § Receptors for Nodal § Abolish antero-

IIA and B posterior polarity of
(Acvr2a and embryo
Acvr2a) § Mesoderm that forms
is entirely
Smad2 § For signal extraembryonic
transduction of
NODAL-like factors

Foxh1 § Partner of Smad2 in

transductional
regulation

Eomesodermin § T-box transcription § Represses E-cadherin Target genes:

factor § Induces Epithelial- Lmx1, Mesp1
mesenchymal transition

Hex § Transcription factor

§ After morphogenetic
movements, become
shifted to anterior end

Cerberus-like1 § Inducing factor § Multiple primitive

(Cerl1) § Inhibit nodal, bmp, streaks form
wnt

dick-kopf1 § Inhibit wnt

(Dkk1)

Lefty § from TGFbeta § Multiple primitive

superfamily streaks form
§ antagonistic to nodal
action
§ left side; assoc with
left-right assymetry
formation w/ nodal
BALCERA, GOROSPE, JAPLIT
MARTINEZG, SALINAS 4BIO5 (Mus Musculus) 23 NOV 2017

Gene Expression When Knocked-Out

WNT3 § At proximal end of § No primitive streak

epiblast or mesoderm
§ Active in primitive
streak
§ important in posterior
body formation

BMP § Expressed at distal § Defective in Additional BMP

extraembryonic primordial germ cells inhibitors work with
ectoderm and embryonic Chrd and Nog to
§ Active in primitive mesoderm
prevent formation of
streak
§ neural induction trunk and tail nervous
system

SOX2 § Expressed all over,

ICM stage onwards

OTX2 § Expressed in whole

epiblast
§ suppressed in
posterior as streak forms

Chordin (Chrd) § In anterior streak and § Prevents formation of

node epiblast forebrain
Noggin (Nog) § Induce neural tissue § Gives defects defects
from epiblast in notochord and
(NEURAL sclerotome
INDUCTION)
§ Mesodermal
dorsalization

BMP4 § induces primordial germ § NO PGC when applied to § w/ PGCs reactivate

cells (PGC) epiblast expression of Oct4,
§ from extraembryonic Sox2
ectoderm

Bhlh-type § asymmetry of the heart

dHAND § expressed on right
eHAND § expressed on left

PITX2, ISL1 § N-Cadherin synthesis,

for counterclockwise
coil in intestines
BALCERA, GOROSPE, JAPLIT
MARTINEZG, SALINAS 4BIO5 (Mus Musculus) 23 NOV 2017

Gene Expression When Knocked-Out

TG737 § genre product § No cilia

POLARIS § symmetrical nodal
§ cilia assembly expression

ZP3 § zona’s glycoprotein as Formation of normal

a specific sperm oocytes but no zona
receptor (infertile)

Cdx2 § Upregulation is activated

by Activation of FGF
signaling (needed in
formation of
trophoectoderm)

TEAD4 § TEF family transcription

factor that expresses Cdx2
§ suppresses expression of
Nanog and Oct4
§ trophoectoderm formation

ELF5 § transcription factor

needed for the maintenance
of cell division
§ upregulated by FGF4 and
NODAL

OCT4, NANOG § pluripotency factors

and SOX2 § Stem cell properties are
dependent on the
upregulation of these
Transcription factors