CHE 309 Intro Bioeng A2 G.

Turcotte
Ryerson University January, 2018

Students must submit their answers through D2L before 11 am on Thursday, January 25, 2018. There is
no need for a cover page but students should use a header similar to the one on this page, with their
names instead of the instructor’s. Please include the questions before answering them.

Question 1. True of False. Please remember that all parts of the sentence must be True for the
sentence to be deemed True (50 marks)

True or
False?
1 Pichia stipitis is a yeast capable of making yeast from wood carbohydrates,
and the nucleus of prokaryotes is the only gene sequence in the cell
2 Liquid nutrients are supplied to cells in a Petri dish, and a piece of skin has
only one type of human cells
3 Stem cells will differentiate into many types of animal cells, and a piece of
plant leaf can produce a whole plant (in the right conditions)
4 Viruses are obligate parasites, and all photosynthetic microbes are algae
5 Methanogens are archaebacteria, and yeasts are fungi
6 Microorganisms are prokaryotes, and all microorganisms are multicellular
7 Eukaryotes have a cell wall, and some prokaryotes have a cell wall
8 Eubacteria are eukaryotes, and the average size of viruses is 0.03-0.2 x 10-
6
m (0.03-0.2 µm)
9 The average size of a prokaryote is 0.5-3 x 10-6m, and cyanobacteria are
eukaryotes called algae
10 Animal cells have no cell wall, and sterols are present in cell membranes of
eukaryotes
11 Animal cells reproduce sexually, and eukaryotes by fission
12 Respiration and oxidative phosphorylation occur in mitochondria, and
protein modifications (N-linked glycosylation) occur in the rough
endoplasmic reticulum
13 Lipid synthesis in prokaryotes occur in the smooth ER, and ribosomes are
the site of protein synthesis
14 Lysosomes, peroxisomes and glyoxysomes are only present in eukaryotes
15 N-linked glycosylation of proteins occurs in the rough ER, and O-linked
glycosylation of proteins occurs in the Golgi body (complex; apparatus)
16 Some prokaryotes and all eukaryotes have vacuoles
17 Both photosynthetic bacteria and eukaryotes have chloroplasts to perform
photosynthesis
18 Molds grown in submerged cultures can produce aggregates 10 times the
size of the filamentous form growing n moist, solid surfaces
19 Fungi are yeasts, and all eukaryotes have vacuoles
20 Using a 20-nm pore size filter for a bacterial solution contaminated by
bacteriophages, healthy bacteria will remain on the filter and the viruses
will all be found in the filtrate
21 The glycosylation of proteins changes the protein structure, function and
stability
CHE 309 Intro Bioeng A2 G. Turcotte
Ryerson University January, 2018

22 Totipotent cells can differentiate to make a complete organism, and

unipotent cells can only reproduce their own type of cells
23 In a batch fermentation, the product is recovered in small amounts along
the fermentation
24 Cyanobacteria are eukaryotes capable of using light energy, and
Agrobacterium tumefaciens infects plant roots
25 Gram-positive cells have strong cell walls containing peptidoglycans, and
Gram-negative cells appear pink because they lost the violet stain (of the
first step of the technique) during the second step

Question 2. Reproduce, here, the linear graph of the textbook Problem 6.3 (part (a) of Question 4 in
Assignment 1, last week):

(a) Add to the graph two vertical lines indicating the beginning and the end of the
exponential phase of growth of the bacterium, and neatly indicate the name of the
phase at the appropriate position. (10 marks)

(b) Justify your chosen locations for the beginning and the end of the exponential phase
of growth (in other words, what proof do you have that the locations are correct?).
(15 marks)

Question 3. Highlight in red each word that you changed in order to make the whole paragraph to
become correct and True (25 marks)

In industry, a mixture of lactic acid bacteria (LAB) is used to ferment a mixture of

carbohydrates. Examples are Lactobacillus sp., Streptococcus sp., and Leuconostoc sp.
One bacteria of yogurt and cheese is L. bulgaricus, whereas L. Mesenteroides serves as
lactic acid yeast in Southern India Idli bread. In other industries, the fungus A. niger
produces citric acid for soft drink production, and ethanolic drinks could be made with
the yeast S. cerevisiae.

Total: /100
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