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Article history: Foodborne diseases remain substantial and safety of food is an important health, social and economical issue.
Received on: 19/04/2013 Food borne illnesses caused by microbial contamination raises concerns to find alternate sources which are safe
Revised on: 02/05/2013 to human and environmental health. This study was investigated to determine the antibacterial activity of broccoli
Accepted on: 23/05/2013 (Brassica oleracea L. var. italica). Various solvent extracts of broccoli were prepared and analyzed for their
Available online: 30/05/2013 phytoconstituents. A total of six food borne bacteria viz., Bacillus cereus ATCC 10876, Bacillus subtilis ATCC
6633, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, Salmonella typhimurium MTCC 3224
and Shigella flexneri ATCC 12022 were tested against the broccoli extracts. Minimum inhibitory concentration
Key words:
(MIC) values of 10 - 320 µg ml-1 were recorded against most of the pathogens with acetone and methanol as the
Broccoli, food borne
potential extracts. B. subtilis ATCC 6633 (15.4 mm) and Bacillus cereus ATCC 10876 (16.3 mm) were found to
pathogens, antibacterial,
be the most sensitive organisms among the pathogens tested.
Brassica oleracea
© 2013 Sibi G et al. This is an open access article distributed under the terms of the Creative Commons Attribution License -NonCommercial-
ShareAlike Unported License (http://creativecommons.org/licenses/by-nc-sa/3.0/).
Sibi et al. / Journal of Applied Pharmaceutical Science 3 (05); 2013: 100-103 101
The extracts were filtered using Whatman No.1 filter The data obtained were statistically analyzed and the results were
paper and concentrated to dryness under reduced pressure in a expressed as means along with standard deviation of three parallel
rotary evaporator and stored in sterile vials at 4°C until used. measurements.
Table. 3: Zone diameter of inhibition (mm) in MIC by well diffusion assay (µg/ml) against Gram positive bacteria.
B. cereus ATCC 10876 B. subtilis ATCC 6633 S. aureus ATCC 6538
10 20 40 80 160 320 10 20 40 80 160 320 10 20 40 80 160 320
Pet - - - - - - 16.01 16.21 17.14 16.07 17.08 NG - - - 5.04 7.85 9.64
Chl - - - 9.33 9.67 10.24 - - - - - - - - - - - -
Eta - - - - - - - - - 3.10 5.31 6.58 - - - - - 4.69
Ace 11.12 11.64 12.20 12.23 13.09 15.61 - - - 3.86 3.03 7.01 - - - - - -
Met - 4.18 6.05 6.35 8.34 11.87 6.14 6.98 7.28 7.37 8.26 11.00 - - - - - -
Aqu - - - - - - - - - - - - - - - - - -
Note: NG - no growth
Table. 4: Zone diameter of inhibition (mm) in MIC by well diffusion assay (µg/ml) against Gram negative bacteria.
E. coli ATCC 8739 S. typhi MTCC 3224 S. flexneri ATCC 12022
10 20 40 80 160 320 10 20 40 80 160 320 10 20 40 80 160 320
Pet - - - - - - - - - - - - - - - - - -
Chl - - - - - - 6.12 6.20 7.41 6.34 7.63 9.21 - - - - - -
Eta - - - - - - - - - - - - - - - - - -
Ace 3.00 3.96 6.74 7.04 9.34 18.1 - - - - - 3.94 4.14 4.52 5.38 5.63 8.11 10.01
Met 2.36 4.08 6.11 6.96 7.57 10.35 - - - - - - 5.00 5.09 5.64 6.67 8.34 10.14
Aqu - - - - - - - - - - - - - - - - - -
S.aureus ATCC 6538 was sensitive to petroleum ether activity, bioactive compounds from broccoli (Brassica oleracea L.
and ethyl acetate extracts with the MIC values of ≥ 160 and ≥ 320 var. italica) have scope for the possible use in food industries to
µg ml-1 but other extracts had no activity against the food borne stay away from food borne pathogens.
pathogen. Previous studies recorded the inhibitory activity of
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