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Hatchability Problem Analysis
H. R. Wilson
Introduction
When a problem occurs in hatchability, usually it can be categorized as a hatchery,
egg handling, or breeder flock problem. If the problem has originated within the
breeder flock, it is probable that it happened at least 4 weeks earlier, assuming 3
weeks of incubation and 1 week of egg storage. This delay in identifying a problem is
costly and may even make it impossible to determine the cause if the effect is of
short duration. It is necessary to identify the problem as early as possible, using
candling at 1 week of incubation and constantly monitoring unhatched eggs, to
minimize the delay in taking corrective measures. Analysis of hatch debris does not
yield definitive diagnoses; however, it is a useful tool for determining the most likely
areas for further examination.
It is of utmost importance for hatchery, egg handling, and breeder farm personnel to
work together as a team to produce top quality chicks and to identify problems when
they occur. Very accurate and complete records of the breeder flock (including egg
production, mortality, morbidity, egg weight, shell quality, hatchability, feed
consumption, and antibody titers) and the egg history from the nest through the
hatchery are essential in providing clues to most hatchability problems. Personnel
should be trained in recognizing problems, identifying causes, and implementing
appropriate corrective measures.
The objective of the following outline is to suggest possible causes, and corrective
measures when appropriate, for some of the signs of trouble observed when
decreased hatchability occurs.
General Comments
The magnitude of the effects of deviations from recommended incubation conditions
(temperature, humidity, turning frequency, ventilation, and egg orientation) is a
function of the severity of the deviation, the length of time of the deviation, and the
age of the embryo at the time of the deviation. The manifestation of abnormalities
and the embryonic age at which mortality peaks occur due to nutritional factors
usually depend upon the severity of the nutrient deficiency, how long the deficiency
has existed, or how long an adequate diet has been fed to the breeders following a
deficiency. Therefore, depletion rate, repletion rate, egg deposition efficiency,
interference from inhibitors, and yolk formation time are factors that contribute to the
effects manifested in embryonic abnormalities and mortality.
2. Sign: Eggs candle clear; broken out eggs show enlarged germinal disc;
no blood. Fertile. Some are termed "blastoderm without embryo." Causes:
1. Eggs stored too long. They should be stored <7 days.
2. Eggs held under poor conditions, temperature too high or too low.
Fluctuating temperatures. Temperature should be 60° to 65°F (15.6° to
18.3°C).
3. Fumigation improper -- too severe or done between 12 and 96 h of
incubation. Incorrectly spraying or foaming eggs with disinfectant.
4. Eggs damaged during handling and transport by jarring, temperature
shock (temperature increased or decreased too rapidly), etc.
5. Eggshell sealed -- respiration inhibited.
6. High temperature in early incubation.
7. Very young or very old breeders.
8. Heredity, inbreeding, chromosome abnormalities, or parthenogenesis.
9. Breeder flock diseases.
10. Failure of a basic organ system to develop normally.
11. Egg wash temperature too high.
12. Egg-borne infections (e.g., salmonella).
13. Drugs, toxins, pesticides, etc.
14. Infrequent or incomplete egg collection.
3. Sign: Eggs candle clear; broken out eggs show blood ring or small
embryo that died before 3 days of incubation; no dark eye visible.Causes:
1. Eggs stored too long or under improper temperature.
2. Fumigation improper -- too severe or done between 12 and 96 h of
incubation.
3. High temperature in early incubation.
4. Low temperature in early incubation.
5. Eggs damaged during transport by jarring, etc.
6. Breeder flock diseases.
7. Old breeders.
8. Embryological development accidents.
9. Inbreeding, chromosome abnormalities.
10. Severe nutritional deficiencies, e.g., biotin, vitamin A, copper, vitamin E,
boron, or pantothenic acid.
11. Frequently associated with a high incidence of infertility.
12. Drugs, toxins, or pesticides.
13. Contamination.
14. Embryos less developed at oviposition, i.e., pre-endoderm or very early
endoderm formation.
9. Sign: Chicks stuck in shell, dry; chicks with shell fragments stuck to
down feathers. Causes:
1. Humidity too low during egg storage, incubation, and/or hatching.
2. Improper egg turning.
3. Cracked eggs or poor shell quality.
13. Sign: Unhealed navel, wet, odorous; mushy, large, soft-bodied, and
lethargic chick. Causes:
1. Omphalitis (navel infection). Contamination from dirty trays, unsanitary
machines or hatchery, dirty eggs, inadequate egg sanitation or fumigation.
2. Low incubator temperature.
3. High incubator or hatcher humidity.
4. Inadequate ventilation.
26. Sign: Small air cell, broad pip area, membrane incompletely cut, red
hocks, edematous chick, unabsorbed albumen, yolk incompletely
retracted, egg weight loss <10%. Causes:
1. High incubator humidity.
2. Very thick shells, as in pullet flocks.
3. Low incubator temperature.
27. Sign: Micromelia (shortened long bones, parrot beak, bent bones);
chondrodystrophy (similar to micromelia). Causes:
1. Heredity, lethal genes.
2. Nutritional deficiencies (biotin or manganese).
31. Sign: Swollen head and back of neck (exudative diathesis - increased
capillary permeability). Causes:
1. Nutritional deficiencies -- vitamin E or selenium.
2. Vitamin D3: Late embryonic mortality (>17 days); stunting; poor skeletal
growth; rickets.
5. Thiamin: Polyneuritis; early mortality peak and late peak =>19 days; many
dead chicks in hatching trays.
11. Folic acid: Bent tibia, syndactylism (toe webbing), flattened head, small eyes,
exposed viscera, parrot beak, other beak defects, stunting; peak embryonic
mortality days >17.
12. Vitamin B12: Edema (especially around eyes), hemorrhages, curled toes, short
beak, poor leg muscle development, dwarfing, fatty liver, enlarged thyroid,
dilated, irregularly shaped heart, head-between-thighs malposition; peak
embryonic mortality during days 8 to 14 (small peak) and 16 to 18.
14. Zinc: Skeletal defects, especially in posterior vertebral column (most common
defect is rumplessness), small eyes, exposed viscera, beak and head
abnormalities, edema. Chicks are weak; will not stand, eat, or drink. Embryonic
mortality can be very high.
15. Calcium: Effects more indirect through poor shell quality, increased egg weight
loss, and increased contamination. Stunted growth, decreased bone
development, and increased mortality tend to occur in later stages. A great
excess of calcium also will cause embryonic abnormalities.
16. Magnesium: Nervous tremor, gasping, and convulsions at hatching.
17. Phosphorus: Abnormal bone formation, stunting; mortality peaks during days
14 to 16.
18. Copper: Blood and circulatory system defects. Mortality peaks during days <3.
20. Selenium: Exudative diathesis; selenium will spare vitamin E. Very high levels
of selenium are toxic: edema of head and neck, twisted legs, necrosis in brain
and spinal cord, short upper beak, missing eyes, protruding eyes, an increase
in malpositions.
21. Molybdenum: >17 ppm in the egg results in 100% mortality by day 12.
22. Lithium: Excess causes high embryonic mortality associated with inhibited
development, eye defects, enlarged aorta, abnormal neural tube.
23. Boron: Excess boron in egg (44 ppm) causes embryonic mortality in early
development and at day 13. Abnormalities similar to those of riboflavin
deficiency. Face, beak, and appendicular skeleton abnormalities.
24. Protein, amino acids: Deficiency, excess, or imbalance of some amino acids
can cause embryonic abnormalities and mortality. Abnormalities include small
or abnormal upper and/or lower beak, disorganized protrusions in the brain,
exposed viscera, twisted and shortened limbs, twisted spine, short body,
degeneration of the eye.
25. Fat, fatty acids: Linoleic acid deficiency: slow development, 75% of embryos
in the head-over-right-wing malposition; mortality peaks during days 1 to 4, 8 to
14, and >21. Lipid transfer from the yolk to the embryo is reduced in the first
few eggs produced by young pullets; this appears to result in increased
embryonic mortality.
27. Microorganisms:
1.
1. Infectious bronchitis: Stunting, retarded lung development, small
heart, enlarged spleen. Small chick resulting from thin, porous shell
and excessive water loss.
2. Newcastle disease: Reduced growth, small amnion, abnormalities in
neural and sensory tissues in early embryo.
3. Botulism: Muscle atrophy, fat accumulation, joint problems, short
upper beak.
4. Staphylococcus: Extensive hemorrhages and tissue damage.
5. Streptococcus: Destruction of the synovial lining of the joints.
6. E. coli: Rots.
7. Aspergillus: Black or dark green rots. Embryo red or dark, dwarfed.
8. S. pullorum, S. gallinarum, and S. typhimurium: Egg transmitted.
Embryonic septicemia, high embryonic mortality, high chick
mortality.
The hatch residue breakout includes all eggs that did not hatch. Candle breakout
and residue breakout should be done weekly or at least every 3 weeks. Regular,
consistent analysis of these breakouts will result in flock histories that can be used to
diagnose hatchability problems, minimize losses, and compare strains, flocks, farms,
hatcheries, and many other variables.
Sample selection and size are important for obtaining valid results from the
breakouts. Samples should be selected to include eggs from representative locations
in setters and hatchers for each flock at each sampling time. Suggested minimums
for sample size include: (1) 10 unhatched eggs from 5 hatcher trays; (2) all
unhatched eggs from 4 hatcher trays per setter or hatcher; (3) all unhatched eggs
from 1,000 set eggs; as well as many others.
Records should include, but not be limited to, the following variables: flock, strain,
farm, date set, machine(s) used, location of eggs in machine, number of eggs set,
number of fertile eggs, number of early dead (0 to 7 days), number of middle dead (8
to 14 days), number of late dead (embryos 15 days or older), age of each embryo,
malpositions (in embryos 19 days or older), number pipping, malformations, number
of eggs contaminated (rots), number of cracked eggs (transfer cracks and others),
unusual egg traits (size, shape, shell quality, cleanliness), number of dead and culled
chicks, and number of live chicks. Clear, accurate records are essential for useful
egg breakout analysis.
Eggs should be removed from the hatcher tray, placed on egg flats, and identified as
to flock, location, etc. The exterior of the egg is examined first for egg traits, pipping,
and location of the air cell. The shell is cracked at the large end, over the air cell, and
a hole opened in the shell and membranes to observe the interior of the egg. If the
egg appears to be infertile or contains a very early dead embryo, the germinal disc
must be located to make a definitive identification of fertility. If the embryo is
relatively small, the egg can be broken into a dish for further examination. Eggs with
late-stage embryos should be observed for pipping into the air cell, then opened with
tweezers or scissors from large end to small end without disturbing the position of
the embryo.
The embryo's position (see earlier discussion on positions), the embryo's age (see
section on development stages), malformations, contamination, and other factors
should be observed and recorded. Comparisons with live embryos of various ages
can be used to train those developing experience in the breakout technique.
References
Abbott, U. K. 1975. Identifying causes of problems in hatchability. Poultry
Digest (November): 446-47.
Anonymous. 1971. Incubation trouble shooting. Denver: Robbins Incubator
Company.
Anonymous. 1991. Hatchery trouble shooting guide. Glastonbury, Conn.: Arbor
Acres, Inc.
Coleman, M. A. 1986. Solving hatchability problems. Poultry
International (December): 12-15.
Hodgetts, B. 1988. Why do your embryos die? Internat. Hatchery Practice 2 (3): 4, 5,
7, 9.
Hodgetts, B. Solving hatchability problems. Information for flock farms and
hatcheries. ADAS Ministry of Agriculture, Fisheries and Food. Wolverhampton, UK.
Landauer, W. 1967. The hatchability of chicken eggs as influenced by environment
and heredity. Monograph I (Revised). Storrs Agricultural Experiment Station, Conn.
McDaniel, G. R. 1990. Hatchability: Many factors affect results. Poultry Digest 49 (9):
20, 22, 24, 28, 30.
North, M. O., and D. D. Bell. 1990. Commercial chicken production manual, 103-34.
New York: Van Nostrand Reinhold.
Patten, B. M. 1964. Foundations of embryology. 2d ed. New York: McGraw-Hill, Inc.
Romanoff, A. L. 1960. The avian embryo. New York: The MacMillan Company.
Romanoff, A. L., and A. J. Romanoff. 1972. Pathogenesis of the avian embryo. New
York: Wiley-Interscience.
Summers, J. D., and S. Leeson. 1985. Poultry nutrition handbook, 119-24. University
of Guelph, Canada: Department of Animal and Poultry Science.
Tullett, S. G., and R. C. Noble. 1989. Understanding the chick embryo (III). Low
hatchability problems in young parent stock. Misset International Poultry (January):
8-9.
Wilson, H. R. 1991. "Physiological requirements of the developing embryo:
Temperature and turning." In Avian incubation, ed. S.G. Tullett, 145-56. Developed
from Poultry Science Symposium Number Twenty-Two. London: Butterworth-
Heinemann.
Wilson, J. L. 1991. Hatching egg breakout methods are explained. Poultry Digest 50
(9): 20, 22, 24, 25.
Wineland, M. J., and J. T. Brake. 1984. Trouble-shooting fertility and hatchability
problems. PS&T Guide No. 34. North Carolina Agricultural Extension Service.
Footnotes
1. This document is CIR1112, one of a series of the Animal Science Department,
Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences,
University of Florida. Original publication date October 2004. Reviewed June 2003.
Visit the EDIS Web Site athttp://edis.ifas.ufl.edu
2. Henry R. Wilson, Professor Poultry Physiology, Department of Dairy and Poultry
Sciences, Cooperative Extension Service, Institute of Food and Agricultural
Sciences, University of Florida, Gainesville FL 32611.
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Egg Candling and Breakout Analysis
PUBLICATION 8134
If a hatch is poor, identify the cause of the trouble as quickly as possible. A poor hatch may
be caused by a failure in fertilization or by excessive mortality of the embryos due to a
variety of factors. (For a more complete list of problems, causes, and possible solutions,
see Common Incubation Problems).
Careful examination of a sample of eggs to diagnose hatching problems must include not
only candling but breakout analysis. Even during problem-free periods, chicken, pheasant,
partridge, and quail eggs should be candled after 5 to 7 days of incubation. Turkey, duck,
and goose eggs—because of their longer incubation periods — should be candled after 8 to
10 days of incubation. The sample should be candled again at transfer to the hatcher, and
dead germs should be broken out and examined. For information on setting up an organized
program for commercial hatchery quality control, see Quality Control Procedures for the
Hatchery (Mauldin 1993).
SAMPLING
If small groups of eggs are set, the most appropriate sample is the entire group. If sets
exceed 300 eggs, a sample of 100 to 200 eggs should be examined. In large hatcheries,
sampling procedures should be carefully planned with the assistance of a statistician or
poultry specialist to optimize the quality of the results and minimize costs. During problem
periods, more frequent candling and analysis may be advisable. To determine true fertility,
breakout analysis is required.
The color photos included in this publication help you to distinguish normal,
healthy embryos from infertile eggs and “early-deads.” Refer to Common Incubation
Problems for causes and possible remedies.
The loss of incubated eggs from modern, high-hatching chicken strains, stored under
optimal conditions, should be no more than 10 percent at the first candling. Losses in
turkey, waterfowl, and game bird eggs may be slightly higher. The mortality measured by
candling and breakout during the first candling normally represents one-third of the total
mortality to be expected. The mortality after the second candling should represent two-
thirds of the total to be expected, with very little mortality during midincubation. Mortality
during midincubation may indicate a dietary deficiency if infected embryos or
developmental abnormalities are not seen. However, the most common nutrient
deficiencies result from marginal vitamin levels in the diet and usually cause weak chicks
that have difficulty hatching, without other symptoms.
When eggs are candled after the first mortality peak at 7 to 10 days, three distinct classes
can be recognized: living normal embryos, blood rings, and clears. If eggs are candled at
transfer, clears should not found unless they were missed on the first candling. Dead
embryos should be found in small numbers. Some of these may be associated with poor or
damaged shells that were not removed during the first candling or that developed after the
first candling. Breakout may reveal infected eggs, which can be detected by their abnormal
color and odor.
Examination of eggs that fail to hatch reveals several types of abnormalities. Look for
malpositioned embryos (other than head under right wing and in the large end of the egg).
Excessively wet or dry embryos indicate incorrect incubator humidity, extended or
improper (dry) egg storage, or poor shell quality. A few genetically abnormal embryos may
be found at this stage, but if numbers are excessive, conduct a more detailed investigation.
LIVING NORMAL EMBRYOS
Use this photo as a reference in determining the developmental age of normal turkey
embryos.
Note the small, centrally located embryo and the developing blood vessels in the
yolksac membrane.
4-day-old normal chicken embryo.
Note the more advanced circulatory system than in the turkey embryo of equal age. The
dark area near the center of the egg is the embryo; the radiating lines are the blood
vessels of the extraembryonic mebranes (yolk sac).
24-day-old normal turkey egg.
The light upper part (left side of photo) is the enlarged air cell that is required for
proper hatching. The dark, lower part contains the embryo. One of the keys to good
poult hatchability is the roper amount of “dry down” at the end of the incubation period.
27-day-old normal turkey egg.
The blood vessels at the lower left of this pipped egg have not yet completely dried up
and are still needed for respiration. Humid conditions must be maintained at this stage
to prevent premature drying of these vessels and death of the poult.
BLOOD RINGS AND DEAD EMBRYOS
These eggs show a ring of blood outlined on the inner surface of the shell when candled.
They usually contain fairly advanced embryos that have died only recently, but they may
also contain a blastoderm without an embryo or a cystic embryo.
Blastoderm without embryo (BWE) chicken egg.
The blood ring (arrow) indicates a dead and probably abnormal embryo from a fertile
egg. The lack of any visible embryo
structure indicates that it is a BWE.
Cystic embryo in chicken egg.
The presence of the blood ring in this egg is symptomatic of the early death of an
embryo. Diagnosis may be made by breakout analysis.
Infertile and blastoderm without embryo (BWE) chicken eggs.
In these chicken eggs that have been candled out, the infertile egg (right) shows no
development. The 21⁄2-day-old BWE egg (left) shows the typical extraembryonic
membranes surrounded by a blood ring.
Dead turkey embryo.
In this turkey egg, candled after 10 days of incubation, the large, dark, round spot and
lack of visible blood vessels are the major indications that the embryo is dead.
Abnormal turkey embryo.
This abnormal embryo, about 8 days old, is from an egg candled after 10 days of
incubation. The abnormally shaped head and mouthparts indicate developmental
disturbances that led to death. Decomposition of tissues, as seen here, is typical of dead
embryos.
CLEARS
The term “clears” refers to eggs that show no development when candled, including
fertile eggs
true infertiles
fertile, no development (FND)
positive development
Under normal circumstances, more than half of all clears are true infertiles. The other half
comprise the dead-embryo categories shown in the photos below; these are not true fertility
problems. Especially where they are abundant, the cause of fertility problems must be
identified so that proper remedial measures can be taken.
FERTILE EGGS
Fertile unincubated turkey egg.
Candling an unincubated egg does not show whether it will develop properly. The
indistinct shadow of the yolk (center) is characteristic of an unincubated egg.
Chicken egg incubated 24 hours.
Note that the yolk shadow is more distinct than in the unincubated egg, and that it
appears near the top, a raised round area where the embryo will develop.
Cracked chicken egg.
Eggs with cracks, which are not always visible without candling, should be excluded
because they are likely to break further and may be contaminated with bacteria or
molds that will infect other eggs.
TRUE INFERTILES
Infertile incubated turkey egg.
In these eggs that have been candled out, the infertile egg (right) shows no devel-
opment, while the 21⁄2-day-old BWE egg (left) shows the typical extraembryonic
membranes surrounded by a blood ring.
FERTILE, NO DEVELOPMENT (FND)
Fertile eggs without development are infrequently seen. Possible causes include increased
frequency of recessive lethal genes in inbred stocks and improper egg storage (see Common
Incubation Problems).
Fertile, preoviposital death, turkey egg.
A rarely seen FND. The egg was incubated for 3 days before being broken out. The
blastodisc (arrow) deteriorated but still has the characteristic shape and size of a typical
fertile blastodisc.
POSITIVE DEVELOPMENT (PD)
This is the most frequently seen condition in fertile clears. The embryo dies early, but the
growth of cells over the surface of the yolk may continue for several days. Under normal
conditions only 1 to 2 percent of the clears are PDs. The incidence increases when eggs are
improperly stored or from abuse during shipping.
Positive development (PD) in turkey egg.
This egg contained a fertile germ (arrow) that began to develop and then stopped.
Candled, it appears to be clear. Note the absence of blood, as compared to the BWE
illustrated above.
Positive development (PD) in turkey egg.
This blastoderm has developed further than the one in the previous photo.The absence
of blood formation characterizes it as a PD.
GLOSSARY
blastoderm without embryo (BWE). When candled, a BWE egg shows a blood ring; on
breakout there are no visible embryo structures.
blastodisc. The small disc-shaped region on the yolk that contains the egg nucleus.
blood ring. Circular blood remnant visible when an egg is candled; signifies that
the embryo has died at a young age.
breakout. Breaking an egg and examining its contents to determine whether
the blastodiscwas fertilized or embryonic structures were present.
candled fertility. The percentage of eggs remaining after clears are removed by candling
compare with true fertility.
candled out. Clear eggs removed from the incubator following candling.
candling. Transluminating an egg with light to determine the presence or absence of a
viable embryo or to look for shell defects before setting.
clears. Incubated eggs that appear clear when candled, indicating that they do not contain a
live embryo.
cystic embryo. Embryo that dies early in gestation; the broken-out appearance is similar to
a BWE except that embryo tissue is visible.
dry-down. The amount of moisture lost from eggs during incubation.
dry-bulb temperature. Temperature measured with a standard thermometer or electronic
sensor; compare with wet-bulb temperature.
embryo. An organism in the early stages of its development before hatching.
fertile, no development (FND). Rarely diagnosed condition in which the blastodisc was
fertilized but died before the egg was laid or before growth could be initiated in the
incubator.
fertile, preovipositional death. Rarely diagnosed condition characterized by a blastodisc
that appears to be fertile but dies before the egg is laid by the hen.
germ, germinal disc. Fertilized blastodisc; the embryo has about 50,000 cells when the egg
is laid.
hatch, percent hatch, hatching percent, hatch of total. Percentage of all eggs set that
hatch whether they were fertile or not (a typical hatch might be 80% to 90%).
hatch of fertile eggs, hatchability. Percentage of fertile eggs that hatch (should be above
85%).
hatcher. Machine used to maintain proper conditions for embryos during the final few
(usually 3) days before hatching.
inbred. Birds or flocks that have some degree of inbreeding.
inbreeding. The result of mating closely related birds, such as father to daughter or
brother to sister. As inbreeding increases, the ability of the stock to reproduce usually
declines.
incubation. Maintaining the temperature and humidity needed to initiate embryo growth
and hatching of avian eggs by a female or using a machine.
incubator, or setter. Machine that maintains proper conditions for incubating or setting
avian eggs.
malposition. Hatching embryo in any position except the head under the right
wing positioned in the large end of the shell; for example, the head under the left wing or
the head between the legs.
parthenogenesis. Development of an egg without fertilization, which occurs at low levels in
chickens and turkeys. Parthenogenic embryos usually die; if the embryo hatches it will be a
male with a diploid (2n) number of chromosomes.
pip. Egg in which the chick has broken the shell in an attempt to hatch; also, the act of
breaking the shell. Chicks may die after pipping or may be unable to get out of the shell.
positive development (PD). Eggs that are candled out as clears because there is no blood
formation; the germ was fertile, but it died soon after cell growth resumed when the egg was
warmed above 80ºF (26.7ºC).
poult. Young turkey.
relative humidity. Measure of the water vapor or moisture in air; can be determined from
the wet-bulb and dry-bulb temperature using a psychometric chart.
set. To place eggs in an incubator or under a female for incubation.
setter. An incubator.
spread. The difference between percent of fertile eggs and percent hatch (a 10% to 12%
spread is typical for chicken eggs).
true fertility. Percentage of hatching eggs that are fertile. This can be determined by
incubation, candling, and breakout of the clears to determine which eggs were fertile or
by breaking out potentially fertile eggs to examine the germinal disc (e.g., a sample might be
examined to estimate fertility of a flock).
true infertility. Lack of true fertility.
wet-bulb temperature. Temperature measured by a standard thermometer equipped with a
wet sock over the bulb. For accurate measurements, air must be moving over the wet sock to
provide evaporation. Electronic sensors are now available to measure the relative humidity
of air in incubators and egg storage rooms.
REFERENCE
Mauldin, J. M. 1993. Quality control procedures for the hatchery. Athens:
University of Georgia College of Agricultural Sciences Cooperative Extension Service.
FOR MORE INFORMATION
You’ll find more information on hatching egg care and incubation in the following ANR
products:
Hatching Egg Sanitation: The Key Step in Successful Storage and Production.
Publication 8120, 2004. Available for free downloading from the
ANR Communication Services Web site, http://anrcatalog.ucdavis.edu/pdf/8120.pdf.
University of California
Agriculture and Natural Resources
Communication Services
6701 San Pablo Avenue, 2nd Floor
Oakland, California 94608-1239
Telephone: (800) 994-8849 or (510) 642-2431; FAX: (510) 643-5470 E-mail
inquiries: danrcs@ucdavis.edu
Publication 8134