Propagation of Ornamental Plants

Vol. 15, № 1, 2015: 35-41

CRYOPRESERVATION OF CHINESE OLD ROSES (ROSA CHINENSIS JACQ.) SHOOT TIPS

BY VITRIFICATION

Qian Liu1, Yan Liu1*, Jin Xu1, Jiaopeng Cui2, Mengxue Jia1, and Yin Shi1

1
Beijing Forestry University, National Engineering Research Center for Floriculture, 35 Tsing Hua East Road, 100083 Beijing,
China, *E-mail: chblyan@163.com
2
Beijing Botanical Garden, Xiangshan Road, Beijing 100093, China

References
Ai P. F., Luo Z. R. (2003). Cryopreservation of in vitro shoot-tips of persimmon and date plum by vitrification and genetic stability
of regenerated plantlets. Scientia Agriculturae Sinica, 37: 2023-2027.
Benson E. E., Harding K. (2012). Cryopreservation of shoot tips and meristems: an overview of contemporary methodologies. In:
Loyola-Vargas V. M., Ochoa-Alejo N. (Eds). Plant Cell Culture Protocols. Humana Press, New York: 191-226.
Benson E. E., Harding K., Johnston J. W. (2007). Cryopreservation of shoot tips and meristems. In: Day J. G., Stacey G. N. (Eds).
Cryopreservation and Freeze-Drying Protocols. Humana Press, New York: 163-183.
Halmagyi A., Pinker I. (2006a). Cryopreservation of Rosa shoot tips: importance of preculture conditions. Acta Horticulturae, 725:
351-356.
Halmagyi A., Pinker I. (2006b). Plant regeneration from Rosa shoot tips cryopreserved by a combined droplet vitrification method.
Plant Cell, Tissue and Organ Culture, 84: 145-153.
Hong S. R., Yin M. H. (2009). High-efficiency vitrification protocols for cryopreservation of in vitro grown shoot tips of rare and
endangered plant Emmenopterys henryi Oliv. Plant Cell, Tissue and Organ Culture, 99: 217-226.
Kami D., Shi L., Sato T., Suzuki T., Oosawa K. (2009). Cryopreservation of shoot apices of hawthorn in vitro cultures originating
from East Asia. Scientia Horticulturae, 120: 84-88.
Kaviani B. (2011). Conservation of plant genetic resources by cryopreservation. Australian Journal of Crop Science, 5: 778-800.
Le Bras C., Le Besnerais P., Hamama L., Grapin A. (2014). Cryopreservation of ex-vitro-grown Rosa chinensis ‘Old Blush’ buds
using droplet-vitrification and encapsulation-dehydration. Plant Cell, Tissue and Organ Culture, 116: 235-242.
Li D. Z., Pritchard H. W. (2009). The science and economics of ex situ plant conservation. Trends in Plant Science, 14: 614-621.
Li M. J., Zhao X. T., Hong S. R., Zhang X. L., Li P., Liu J., Xie C. H. (2009). Cryopreservation of plantlet nodes of Dioscorea op-
posita Thunb. using a vitrification method. Cryoletters, 30: 19-28.
Lynch P. T., Harris W. C., Chartier-Hollis J. M. (1996). The cryopreservation of shoot tips of Rosa multiflora. Plant Growth Regu-
lation, 20: 43-45.
Murashige T., Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum,
15: 473-497.
Niino T., Sakai A., Yakuwa H., Nojiri K. (1992). Cryopreservation of in vitro-grown shoot tips of apple and pear by vitrification.
Plant Cell, Tissue and Organ Culture, 28: 261-266.
Niino T., Tashiro K., Suzuki M., Ohuchi S., Magoshi J., Akihama T. (1997). Cryopreservation of in vitro grown shoot tips of cherry
and sweet cherry by one-step vitrification. Scientia Horticulturae, 70: 155-163.
Ozudogru E. A., Previati A., Lambardi M. (2010) In vitro conservation and cryopreservation of ornamental plants. In: Jain S. M.,
Ochatt S. J. (Eds). Protocols for In Vitro Propagation of Ornamental Plants. Humana Press, New York: 303-324.
Pawlowska B. (2012). Cryopreservation of Rosa canina and R. rubiginosa apical buds by the droplet vitrification method. Acta
Horticulturae, 937: 905-909.
Pawlowska B., Bach A. (2011). Cryopreservation by encapsulation-dehydration of in vitro grown shoot buds of Rosa ‘New Dawn’.
Acta Horticulturae, 908: 303-307.
Pawlowska B., Szewczyk-Taranek B. (2014). Droplet vitrification cryopreservation of Rosa canina and Rosa rubiginosa using shoot
tips from in situ plants. Scientia Horticulturae, 168: 151-156.
Preetha T. S., HemanthaKumar A. S., Krishnan P. N. (2013). Shoot tip cryopreservation by vitrification in Kaempferia galanga L.,
an endangered, overexploited medicinal plant in Tropical Asia. Journal of Pharmacy and Biological Sciences, 8: 19-23.
Previati A., Benelli C., Da Re F., Ozudogru A., Lambardi M. (2008). Micropropagation and in vitro conservation of virus-free rose
germplasm. Propagation of Ornamental Plants, 8: 93-98.
Quest-Ritson C., Quest-Ritson B. (2003). The American Rose Society encyclopedia of roses. Dorling Kindersley, London, 290 pp.
Sakai A., Engelmann F. (2007). Vitrification, encapsulation-vitrification and droplet-vitrification: a review. Cryoletters, 28: 151-172.
Towill L. E., Jarret R. L. (1992). Cryopreservation of sweet potato (Ipomoea batatas [L.] Lam.) shoot tips by vitrification. Plant
Cell Reports, 11: 175-178.
Uragami A., Sakai A., Nagai M., Takahashi T. (1989). Survival of cultured cells and somatic embryos of Asparagus officinalis
cryopreserved by vitrification. Plant Cell Reports, 8: 418-421.
Vandenbussche B., De Proft M. P. (1998). Cryopreservation of in vitro sugar beet shoot tips using the encapsulation-dehydration
technique: influence of abscisic acid and cold acclimation. Plant Cell Reports, 17: 791-793.
Verleysen H., Samyn G., Van Bockstaele E., Debergh P. (2004). Evaluation of analytical techniques to predict viability after cryo-
preservation. Plant Cell, Tissue and Organ Culture, 77: 11-21.
Verleysen H., Fernandes P., Pinto I. S., Van Bockstaele E., Debergh P. (2005). Cryopreservation of Robinia pseudoacacia. Plant
Cell, Tissue and Organ Culture, 81: 193-202.
Wang G. L. (2003). History of roses in cultivation. In: Roberts A. V. (Ed.). Encyclopedia of Rose Science, vol. 1. Amsterdam, El-
sevier: 387- 395.
Wang Q. Z., Lin L. H., Dong W. X. (2009). Cryopreservation of Rose in vitro shoot tips by vitrification. Journal of Shenyang Agri-
cultural University, 40: 156-159.
Wang Z. C., Deng X. X. (2001). Cryopreservation of Citrus shoot tips by vitrification and regeneration. Acta Horticulturae Sinica,
28: 301-306.
Wang Z. C., Lei J. S., Zhang C. W., He Y. X. (2013). Analysis genetic stability in Prunus humilis Bunge. Plants after cryopreserva-
tion twice. Advances in Forestry Letters, 2: 67-75.
Zhang Z. S., Zhu X. Z. (2006). Rose of China. China Forestry Press, Beijing, 170 pp.