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Critical Ratio of Calcium and Boron in Maize Shoot for Optimum Growth
Shamsa Kanwal a; Rahmatullah a; Tariq Aziz a; Muhammad Aamer Maqsood a; Najam Abbas a
a
Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan
To cite this Article Kanwal, Shamsa, Rahmatullah, Aziz, Tariq, Maqsood, Muhammad Aamer and Abbas, Najam(2008)'Critical Ratio of
Calcium and Boron in Maize Shoot for Optimum Growth',Journal of Plant Nutrition,31:9,1535 — 1542
To link to this Article: DOI: 10.1080/01904160802244530
URL: http://dx.doi.org/10.1080/01904160802244530
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Journal of Plant Nutrition, 31: 1535–1542, 2008
Copyright © Taylor & Francis Group, LLC
ISSN: 0190-4167 print / 1532-4087 online
DOI: 10.1080/01904160802244530
ABSTRACT
Boron (B) deficiency is widely reported in alkaline calcareous soils of the world, in-
cluding Pakistan. High calcium (Ca) content in such soils can affect the availability and
utilization of B by plants. Effect of applied B at different levels of Ca addition on maize
was studied in hydroponics. Four maize cultivars (‘EV-5089’, ‘SWL-2000’, ‘EV-6089’,
and ‘Sultan’) were grown at three levels of Ca (0.25 mM, 1 mM, and 2 mM) and two
levels of B (0 and 25 µM). Application of both the nutrients increased shoot dry mat-
ter production. However, application of Ca antagonized the B concentration in shoot
of four maize cultivars. A curvilinear relationship existed between Ca/B ratio in shoot
and relative shoot dry matter of maize cultivars. Implication of using of Ca/B ratio for
managing commonly occurring B deficiency in calcareous soils is suggested.
INTRODUCTION
Boron (B) is an essential micronutrient required for normal growth and develop-
ment of higher plants. World-wide B deficiency is one of the major constraints
to crop production (Sillanpae, 1982). Soils of Pakistan are generally alkaline
and calcareous in nature and micronutrient deficiencies especially of zinc (Zn),
iron (Fe), and B are reported in various parts of the country (Rashid and Rayan,
2004). Boron deficiency was reported in 49% of the soil samples collected
1535
1536 S. Kanwal et al.
from twenty districts of Punjab for an FAO study (Sillanpae, 1982; Rashid
et al., 1997).
Boron availability in soil is affected by several factors including soil tex-
ture, nature of clay minerals, pH, liming, organic matter, interrelationships with
other elements, and environmental conditions like moderate to heavy rainfall,
dry weather, and high light intensity (Moraghan and Mascagni, 1991). Pres-
ence of free calcium carbonate (CaCO3 ) in alkaline calcareous soils affects the
availability of B to crop plants. In addition to its effect on soil pH, calcium
carbonate also acts as an important B adsorbent in calcareous soils (Goldberg
and Forster, 1991). Boron adsorption is more on soils having higher calcium
carbonate content (Elrashidi and O’Connor, 1982).
Crop plants differ in their B requirement. In general, dicots have more of
a B requirement (20–70 mg kg−1 ) than monocots (5–10 mg kg−1 ) (Marschner,
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to 40 mesh with a Wiley mill fitted with stainless steel blades and chamber.
The ground samples of shoot were dry ashed at 600◦ C for 12 hours. After dry
ashing, samples were digested in sulfuric acid (H2 SO4 ), filtered, and analyzed
for B concentration by UV-Visible spectrophotometer (Shimadzu, UV-1201,
Shimadzu, Columbia, MD) at 420 nm wavelength. Calcium concentration in
plant digest was determined by atomic absorption spectrophotometer (Perkin
Elmer Aanalyst 100, Perkin Elmer, Waltham, MA).
The data regarding shoot dry matter, B uptake and Ca uptake were statisti-
cally analyzed using PC based MSTAT-C (Russel and Eisensmith, 1983). Two
factors, ANOVA and LSD, were used to separate treatment means.
There was a highly significant (P < 0.01) main and interactive effect of addition
of Ca and B and cultivars on shoot dry matter production by the four maize
cultivars. Shoot dry matter of the four maize cultivars ranged from 0.68 to
3.25 g plant−1 (Table 1). There was a significant constant increase in shoot dry
matter production of the four maize cultivars by adding Ca and B to the root
medium. There was no general trend for increasing shoot dry matter yield in
the four maize cultivars at the two levels of B application along with low (0.25
mM Ca), medium (1 mM Ca), and high levels (2 mM Ca) of Ca. However,
a significant interaction (P < 0.01) among Ca, B, and maize cultivars had
a profound effect on relative shoot dry matter production. For example, B
application significantly (P < 0.01) increased shoot dry matter production 62
to 159% of the maize cultivars at various levels of Ca addition used in this study.
Similarly, application of higher level of Ca at different levels of B significantly
increased shoot dry matter production 27 to 111% as compared to its lowest
level of 0.25 mM Ca application.
1538 S. Kanwal et al.
Table 1
Shoot dry matter of maize cultivars (mean of 9 replications)
Ca × B × Cultivar
There was a significant (P < 0.01) main and interactive effect of Ca, B, and
cultivar on concentration and uptake of Ca in maize shoot (Tables 2 and 3).
Addition of Ca to the growth medium significantly (P < 0.01) increased the
Ca concentration in shoot. The calcium concentration ranged from 1.91 to 3.78
mg g−1 of shoot dry matter in four maize cultivars. Boron application had a
Table 2
Concentration of calcium in shoot of four maize cultivars (mean of 9 replications)
+B at 25 +B at 25 +B at 25
Maize cultivars –B µM –B µM –B µM
Table 3
Total uptake of calcium in shoot of four maize cultivars (mean of 9 repeats)
Ca × B × Cultivar
Table 4
Concentration of boron in shoot of four maize cultivars (mean of 9 repeats)
Table 5
Total uptake of boron in shoot of four maize cultivars (mean of 9 repeats)
Ca × B × Cultivar
Figure 1. Relation between relative shoot dry matter production and Ca:B ratio in
shoots.
Calcium Boron Ratio for Optimum Growth 1541
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