Electronic supplementary material

Interaction of Nano-TiO2 with Lysozyme: Insights into the Enzyme

Toxicity of Nano-sized Particles

0.36
1
2
0.30 3
4
5
0.24 6
A280 nm

0.18

0.12

0.06

0.00
0 100 200 300 400 500
Time (min)

Fig. S1 Effect of time on the absorbance of supernatant. The suspensions initially contained 0.139, 0.417, 0.694,
0.972, 1.250, 1.736 μM lysozyme (from 1 to 6), 140 mg/L nano-TiO2 and 0.15 M NaCl solutions at pH 7.4

40

30
MRE (10 degs cm mol )
-1

4
2

20

10
3

1
0

-10

-20
200 220 240
Wavelength (nm)
Fig. S2 CD spectra of the suspensions containing 1.38 μM lysozyme and 0, 5, 20 and 40 mg/L nano-TiO2 (from
curve 1 to 4) at pH 7.4