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Name: Enriquez, Jigs 4Bio8

Exercise 3: Neurophysiology of Nerve Impulses: Activity 7: The Action Potential: Conduction Velocity Lab Report

Pre-lab Quiz Results You scored 100% by answering 5 out of 5 questions correctly.

1. An action potential can be propagated along an axon because there are

2. The units of conduction velocity are

channels in the membrane.

3. Which of the following will affect axonal conduction velocity?

You correctly answered: c. both the diameter of the axon and the amount of myelination

4. Which of the following describes an A fiber?

You correctly answered: a. large diameter, heavily myelinated

5. Which of the following describes a C fiber?

You correctly answered: c. small diameter, unmyelinated

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Experiment Results Predict Question:

Predict Question 1: How will the conduction velocity in the B fiber compare with that in the A Fiber? Your answer : b. The conduction velocity in the B fiber will be slower because the B fiber has a smaller diameter and less myelination.

Predict Question 2: How will the conduction velocity in the C fiber compare with that in the B Fiber? Your answer : b. The conduction velocity in the C fiber will be slower because the C fiber has a smaller diameter and less myelination.

Stop & Think Questions:

3. Note the difference in time between the action potential recorded at R1 and the action potential recorded at R2. The

distance between these sets of recording electrodes is 10 centimeters (0.1 m).

Convert the time from milliseconds to seconds, enter the time (in seconds) in the field below, and then click Submit Data to display your results in the grid. You answered: 0.002 sec

4. Calculate the conduction velocity in meters/second by dividing the distance between R1 and R2 (0.1 m) by the time it took

for the action potential to travel from R1 to R2.

Enter the conduction velocity in the field below and then click Submit Data to display your results in the grid. You answered: 50 m/sec

7. Note the difference in time between the action potential recorded at R1 and the action potential recorded at R2.

Convert the time from milliseconds to seconds, enter the time (in seconds) in the field below, and then click Submit Data to display your results in the grid. You answered: 0.01 sec

8. Calculate the conduction velocity in meters/second by dividing the distance between R1 and R2 (0.1 m) by the time it took

for the action potential to travel from R1 to R2.

Enter the conduction velocity in the field below and then click Submit Data to display your results in the grid. You answered: 10 m/sec

11. Note the difference in time between the action potential recorded at R1 and the action potential recorded at R2.

Convert the time from milliseconds to seconds, enter the time (in seconds) in the field below, and then click Submit Data to display your results in the grid. You answered: 0.1 sec

12. Calculate the conduction velocity in meters/second by dividing the distance between R1 and R2 (0.1 m) by the time it

took for the action potential to travel from R1 to R2.

Enter the conduction velocity in the field below and then click Submit Data to display your results in the grid. You answered: 1 m/sec

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Experiment Data:

 Axon Type Myelination Stimulus Distance From Time Between Time Between Conduction Voltage (mV) R1 to R2 (m) APs (msec) APs (sec) Velocity (m/sec)
 A fiber Heavy 30 0.1 2 0.002 50 B fiber Light 30 0.1 10 0.01 10 C fiber None 30 0.1 100 0.1 1

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Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly.

1. Action potential conduction velocity is fastest in which of the following fibers?

You correctly answered: a. A fibers

2. Action potential conduction velocity is slowest in which of the following fibers?

You correctly answered: c. C fibers

3. Why did the timescale have to be changed to measure the conduction velocity of the C fibers?

You correctly answered: b. The total time shown on the oscilloscope would have been too short to see the action potential at

 R2. 4. The axons from touch fibers are A fibers, and the axons from pain fibers are C fibers. When you stub your toe, which

would you expect to perceive first? You correctly answered: b. your toe touching something

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Review Sheet Results

1. How did the conduction velocity in the B fiber compare with that in the A Fiber? How well did the results compare with

The B Fiber indeed was slower by 40 m/sec most likely due to less myelination as well as smaller diameter.

2. How did the conduction velocity in the C fiber compare with that in the B Fiber? How well did the results compare with

Similar to the B and A Fiber comparison, C Fiber was slower which again is likely due to less myelination and smaller diameter.

3. What is the effect of axon diameter on conduction velocity?

Larger axon diameter will have less resistance to current flow as well as provide a larger surface area.

4. What is the effect of the amount of myelination on conduction velocity?

Higher myelination will provide better insulation thus allowing decreased capacitance of the membrane which means that the conduction spreads down the channel of the axon more quickly.

5. Why did the time between the stimulation and the action potential at R1 differ for each axon?

The time between the simulation and the action potential at R1 differ for each axon because the axon diameter and amount

of myelination varied for each axon.

6. Why did you need to change the timescale on the oscilloscope for each axon?