Eisenthal-Cornish Transform

 Eisenthal and Cornish-Bowden (1974) who rearrange the hyperbola to give

maximum velocity, V in terms of remaining parameters
 Equation is based on the reciprocal form of Michaelis-Menten equation at constant
total enzyme concentration E t level:

Eisenthal And Cornish-Bowden Plot Equation

at constant
Vo and S o  , a plot of Vmax against K m will be linear

Vmax S o 
Vo 
K m  S o 

1 K  S o 
 m
Vo Vmax S o 

Vmax K m  S o 

Vo S o 
Vmax K m S o 
 
Vo S o  S o 
Vmax K m
 1
Vo S o 

Eisenthal‒Cornish-Bowden Direct Plots

Pairs of v, [S] data are plotted as follows:
• Values of v along the y axis and the negative values of [S]
along the x axis.
• For each pair, one then draws a straight line connecting the
points on the two axes and extrapolates these lines past their
point of intersection.
• When a horizontal line is drawn from the point of intersection of these line to the y axis, the value
at which this horizontal line crosses the y axis is equal to Vmax.
• Similarly, when a vertical line is dropped from the point of intersection to the x axis, the value at
which this vertical line crosses the x axis defines Km. Eisenthal—Cornish-Bowden direct plot of
enzyme kinetic data.