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BIO 101 Laboratory

This serves as a supplement in the laboratory.


Don’t use this in your lecture class.

EXERCISE VII
PLANT TISSUES

A. MERISTIMATIC TISSUES / MERISTEMS


 composed of mitotically dividing cells
 responsible for elongation & widening the diameter of plant organs
1. Apical Meristem
 found at the tips of stems (shoot apical meristem) & roots (root apical meristem); produce
primary tissues
 account for primary growth → elongation of roots & shoots

Root Apical Meristem Shoot Apical Meristem


(RAM) (SAM)

2. Intercalary Meristem
 occur between mature tissues
 most common in grasses → occur at the bases of the nodes
 help regenerate parts removed by grazing herbivores
 for rapid elongation of the internodes in monocots

3. Lateral Meristem
 cylindrical meristems that form the subapical regions of the roots & shoots of woody plants
 produce secondary growth → increases the girth of the plant
 secondary growth makes the plant sturdier enabling it to grow taller & intercept light
 it includes the vascular cambium & cork cambium (also, phellogen)
 vascular cambium → forms the wood (secondary xylem) & secondary phloem
 cork cambium (phellogen
phellogen)
phellogen → forms the periderm which in turn forms a layer of dead,
suberized cells that protects the inner tissues of the secondary plant body

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

Intercalary Meristem Lateral Meristem


(found only in monocots) (Vascular cambium & Cork Cambium)

B. PROTECTIVE TISSUES / DERMAL TISSUES


 comprise the outermost layer of the plants
1. Epidermis
 dermal tissue that covers the primary body of the plant, protecting the underlying tissues
 regulates the movement of gases between the plant and the atmosphere
 sheet of cutinized cells covering the plants to protect it against rapid water loss &
mechanical injury
 modified epidermal cells:
 Guard Cells
 kidney-shaped in dicots
 dumbbell-shaped in monocots
 Trichomes
 single-celled or multicellular outgrowths of epidermal cells
 found in leaves, stems, flowers, & fruits
 e.g.: cotton fibers → from the epidermis of cotton seeds
2. Cork
 the outermost part of the periderm
 secondary tissue produced by the cork cambium

Epidermis with Guard Cells & Trichomes Cork


Stomata
Stomata

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

C. FUNDAMENTAL/ GROUND TISSUES


 more variable in function & type
 it constitute the mesophyll in leaves & the cortex & pith in stems & roots
1. Parenchyma
 isodiametric or polyhedral cells with prominent protoplast & thin walls
 common examples are:
 Chlorenchyma – contains chloroplasts
 Aerenchyma – cells with prominent intercellular spaces
2. Collenchyma
 consists of cells with unevenly thickened cell walls
 functions for support
3. Sclerenchyma
 consists of cells which are devoid of protoplast upon maturity but has thick lignified walls
 two types of cells:
 Sclerids – short & polygonal
 e.g.: stone cells in pears gives it a gritty texture (rough)
 Fibers – long and with tapering ends
 e.g.: hemp fibers for making ropes

Parenchyma Collenchyma Sclerenchyma


Distinguishing  made up of  made up of collenchyma  made up of fibers (long
Features parenchyma cells → cells → elongated in slender cells typically
usually many sided shape occurring in strands) &
sclerids (variable shape,
shorter than fibers)
Cell Wall  primary or secondary;  unevenly thickened  primary & thick secondary;
may be lignified, primary cell wall only; usually lignified
suberized, or cutinized not lignified
 alive at maturity  alive at maturity  maybe alive or dead at
maturity
Location on  Throughout the plant  beneath the epidermis in  Fibers: usually associated
the Plant Body body young elongating stems with xylem & phloem; in
often seen as a cylinder leaves in monocots;
of tissue or only in sometimes in cortex of
patches in ribs along stems
veins in some leaves  Sclerids: throughout the
plant
Function  for metabolic processes  support in primary plant  Fibers → for support
such as respiration & body  Sclerids → for protection &
photosynthesis; storage mechanical support
& conduction; wound
healing & regeneration

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

Parenchyma Cells Chollenchyma Cells Sclerenchyma Cells

Sclerenchyma (Fiber Cells) Sclerenchyma (Stone Cells)

D. Vascular / Conducting Tissues


 Vascular tissues are complex → i.e. composed of four types of cells
1. Xylem
 for transport of water & dissolved minerals absorbed by the roots from the soil
 composed of fibers, parenchyma cells, vessels (large), & tracheids (small, devoid of
protoplast)
 Vessels & Tracheids
 main water-conducting elements
 exhibit secondary wall thickenings which can be annular, spiral, scalariform,
reticulate, or pitted

Xylem showing vessel elements & tracheids

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

in longitudinal (left) and transverse (right) sections.

2. Phloem
 for transport of food materials from the leaves to all plant parts
 in woody stems, phloem tissues constitute the greater bulk of the bark
 consists of fibers, parenchyma cells, & sieve tube cells (devoid of protoplast)
 Sieve Tube Cells
 main food-conducting elements
 thick-walled & provided at both ends with perforated sieve plates
 companion cells → associated with sieve tube cells

Phloem showing sieve tube members & companion cells


in longitudinal (left) & transverse (right) sections.

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

PLANT ORGANS
Vegetative Plant Organs:
1. Roots
2. Stems
3. Leaves

Reproductive Plant Organs:


1. Flowers
2. Fruits
3. Seeds

A. ROOTS
Root System

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

Monocot Root

Young Eudicot Root

1. Epidermis – uncutinized cells with root hairs (to facilitate absorption)


2. Cortex – multi-layered parenchyma cells:
 Endodermis = inner most layer (endodermal cells)
 Casparian strip = suberized
 Passage cells = for water transport
3. Stele – innermost cylinder
 Pericycle – made up of parenchyma cells; gives rise to the vascular cambium &
secondary/lateral roots
 Xylem – 3-4 radial rows of thick walled cells alternating with thin phloem cells
 Vascular cambium – meristimatic zone between phloem & xylem
 Phloem – thin-walled; food conducting cells alternating with xylem rays
 Pith – if present, composed of parenchyma cells

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

B. Stems
Morphology

Monocot stem (cross section)

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

C. Leaf
Morphology Venation

Monocot Eudicot
(Parallel) (Netted/Reticulated)
Monocot Eudicot

Simple Leaf

Pinnately Compound Leaf Palmately Compound Leaf

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

Leaf Arrangement

Whorled
Alternate Opposite

Monocot:

Eudicot:

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

D. Flower
Morphology

 Complete Flower – has all 4 parts (sepals, petals, pistil, stamen)


 Incomplete – lacks one or more of the 4 parts
 Perfect Flower (bisexual) – has both stamens & carpels/ovary
 Imperfect Flower (unisexual)– has stamen or carpel but not both
Imperfect flower = Incomplete Flower
 Monoecious – male & female reproductive parts in separate flowers (e.g.: corn)
 Dioecious – male & female reproductive parts in separate plants (e.g.: papaya)

Inflorescence

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

E. Fruit

 matured / ripened ovary


 holds & protects seeds
 Simple Fruit – developed from a single ovary
 Aggregate – several ovaries of a flower (sugar apple)
 Multiple – several ovaries of several flowers (pineapple)

TERMS:
 Pericarp - the fruit wall (derived from the ovary of the pistil) which is composed of the exocarp,
mesocarp and endocarp (the three layers are not always recognizable, ex. dry fruit)
 Endocarp - the innermost layer of the pericarp (often hard, stony or papery)
 Mesocarp - the middle layer of the pericarp (often fleshy)
 Exocarp - the outermost layer of the pericarp (often like a skin or peel). (peach)

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

Types of Fruits

Fleshy

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

Dry Fruits

Dry Dehiscent

Dry Indehiscent

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BIO 101 Laboratory
This serves as a supplement in the laboratory.
Don’t use this in your lecture class.

F. Seed
Albuminous (Endospermic) Seed :

Exalbuminous (Non-
(Non-endospermic) Seed

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