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LIST OF TESTS PERFORMED WITH EQUIPMENTS / METHODS USED

A. HEMATOLOGY SECTION :

Tests Method Equipments used


Hematocrit - Manual Microhematocrit centrifuge by
Hettich
WBC count/Differential - Manual -Biological Light Microscope by
count Olympus CH2
- Hemocytometer
- Thoma pipette
- Pipet shaker
- Hematologic stains
- Tally counter
Hemoglobin - Cyanmeth. - Chemistry analyzer by
spectrophotometric Humalyzer 2000
Platelet count - Direct Method Unopette system by BD
Biological Light Microscope by
Olympus CH2
Hematologic stain
- Indirect Method Light Microscope

Reticulocyte count - Direct Method Light Microscope by Olympus


CH2
BCB stain
Tally counter
Clotting Time / Bleeding - Ivy method/ Lee and BP apparatus
Time White Water bath / filter paper/ timer
ESR - Modified westergen Disposable westergren tube
method ESR tube stand / timer
Blood Typing( ABO/Rh ) - Slide and tube Typing sera/ slides tubes
method NSS
Light Microscope
Prothrombin Time ( PT ) - Automated Option 4 plus by Biomerieux
Micropipettes by BRAND
Activated partial - Automated Option 4 plus by Biomerieux
Thromboplastin
time(APTT)
Malarial smear - Slide method Biological Light Microscope by
Olympus CH2
Wright’s /Giemsa Stain
Hgb/Hct/RBC/WBC - Automated CELL-DYN 1200
MCV/MCH/MCHC/RDW MYTHIC 22
Platelet ct

B. PARASITOLOGY / URINALYSIS ( MICROSCOPY ) :

Tests Method Equipments


Urinalysis Physical / Gross
- Chemical tests : - Urine reagent strips 10
Glucose/ketone/albumin/nitrites/sp. parameters
Gravity/urubilinogen/ - Uryxxon Relax
pH/Hgb/Leukocyte esterase (Machery-Nagel)
automated urine strip
reader

- Microscopic Biological Light


Microscope
Clinical centrifuges by
Hettich
Urine HCG - -Immunochromatographic assay One step kit
Urine creatinine/ T. - -Endpoint and Humalyzer 2000
Protein kinetic(spectrophotometric) Chemistry analyzer
method
Microalbumin - Chemical test Micral Test Strip by
Roche

Fecalysis Physical/ Gross


- Microscopic exam for Biological Light Microscope
parasites/ ova NSS/ slides/ Lugol’s soln.
Occult Blood test - Guaiac method: test One step kit
cards
Body Fluid Analysis - Chemical tests : -Humalyzer 2000 Chemistry
Spectrophotometric analyzer by Human
- Chemwell/Chemray 240/Selectra
- Microscopic tests: junior
Cell count / diff count. -Biological Microscopes by
Olympus
-Stains by Hemacolor(merck)
C. CLINICAL CHEMISTRY

Tests Method Equipments


a.)Semi-Automated
1. Glucose - Enzymatic Chemistry Analyzers:
2. Total Cholesterol/HDL LDL - Enzymatic -Visual by Biomerieux
3. Triglycerides - Enzymatic - Humalyzer Jr.
4. Uric Acid / Urea - Enzymatic - Humalyzer 2000
5. Creatinine - Enzymatic - Microlab 300
6. SGOT/SGPT/ALP/ACP/LDH - Enzymatic b.)Automated machine
Gamma GT/Amylase/ CK- - Kinetic - Chemwell
MB - Chemray 240
7. Bilirubin - Colorimetric - Selectra junior
8. CSF/ Urine/ T.Protein - Colorimetric c.) Glucose monitoring
9. Phosphorus/Magnesium - Colorimetric device by Medisense
Calcium - ISE Optium / Medisense
10. Serum T.Protein/ albumin - Colorimetric Precision
11. Na/K/Cl - Ion selective - Easylyte plus by Medica
Electrode - Gemlyte
12. pH /pO2/pCO2 - ABL 500 blood gas
analyzer

13. HBA1c - HPLC - D10 (Biorad)


- Reflectance - LabonaCheckA1c
(Ceragem)
14. Troponin – T/ D-Dimer - Reflectance - Nycocard reader II
- Cardiac reader by Roche
15. Others: send out to referral
laboratories.

D. SEROLOGY :

Tests Method Equipments


- Biological Light
1. HBsAg Screening -Immunochromatographic Microscope by Olympus
2. Anti-HCV Screening assay CH2
3. Anti-HBs Screening - Clinical Rotators by
4. Anti-HIV Screening - s/a Fisher
5. RPR - s/a - Incubators by Melag
6. T.pallidum antibody - Carbon Agglutination - Micropipettes by Brand /
- Immunochromatographic Timers
assay

7. C-RP (quali.) -Latex Agglutination

8. C-RP (quanti ) -Reflectance ( Immuno.) - Nycocard reader II


9. Rheumatoid Factor - Latex agglutination

10. IgG/IgM antibody - Strip, pre doted antigen


to S.Typhi -Immunochromatographic
assay
11. Antigen/IgG/IgM to - Immunochromatographic
Dengue virus assay

12. ASO titer -Latex Agglutination

BLOOD BANKING :

Tests Method Equipments


1. 3 phase- -Slide/tube method -Biological Microscopes by
Crossmatching Olympus CH2
- Incubators / waterbath
- AHG / 22% Bovine Albumin
- Blood Bank ref. by Jewett
2. Blood Component -Manual ( whole blood and - Blood separation stand by
preparation Packed red cells) Terumo

-Sealing clips by Terumo

3. Phlebotomy of -Double and single blood bags


Donors -Blood collection scale by
Terumo

E. MICROBIOLOGY :

Tests Method Equipment


Manual Method -Biological Microscopes by
Olympus CH2
-Biological Incubators by Melag

1. Specimen collection - Transport media by Bacte/Alert


(BioMerieux)

2. Media preparation - Speed Autoclave


- Triple beam balance
- Hot plate by Corning
- BAB Thiogycollate,
BHI,Mac,TSB,SIMMH
media
3. Biochemical Tests -Prepared media for gram - Crystal ID by BD
negative ID - Incubators by Melag

- Latex agglutiunation for - Slidex Staph


Staph. Aureus ID

- Latex agglutination for - Slides Strep by


Strep. groupings BioMerieux
- Biological Microscope by
Olympus CH2

4. Antimicrobial tests Diffusion method - Antibiotic disks by


MAST dx
--MH agar
--Plates by Greiner
5. Grams Staining and - Grams stain-slide
AFB staining Manual Method Cold - Kinyoun Stains
staining - Biological Microscopes
6. KOH mount Slide method - KOH reagent
- Warm plates by Melag
- Biological Microscopes

F. HISTOPATHOLOGY :

Tests Method Equipments


1. Tissue processing - Generally as Manual -Microtome by Leica
2. Cytology: method -Paraffin Oven by Fisher
- Cell Block -Centrifuges by Clay Adams
- Paps smear -Glasswares by O.Kindler
-Blades by Feather
-Stains by Merck
-Biological Microscopes by
Olympus CH2

Noted by :

Dr. Glenn C. Battad Dr. Modesty A. Leaňo


Medical Director Pathologist/Technical Supervisor

Roger P. Villareal, RMT


Chief, Medical Technologist

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