User: USER Path: C:\Programmer\BMG\Omega\User\Data\

Test Name: MTT FRANZI Date: 17-05-2018

ID1: 180517 MTT day 14_3TC and Tennofovir
Absorbance Absorbance values are displayed as OD

1. Raw Data (490)

1 2 3 4 5 6 7 8 9
A
B 0.85 1.553 1.797 1.765 1.361 0.655 1.473 1.481
C 1.004 0.592 1.017 1.281 1.679 0.409 0.972 1.456
D 0.968 1.291 1.048 1.312 2.008 0.32 1.835 0.249
E 1.098 1.03 1.974 1.555 0.951 0.163 1.695 1.518
F 1.387 1.602 0.733 0.657 1.402 0.474 1.636 1.815
G 1.047 1.048 1.748 1.885 2.02 0.681 1.851 1.893
H

2. Raw Data (650)

1 2 3 4 5 6 7 8 9

B 0.154 0.451 0.515 0.444 0.423 0.134 0.363 0.34

C 0.465 0.148 0.159 0.187 0.409 0.093 0.159 0.497

D 0.179 0.298 0.192 0.33 0.552 0.133 0.476 0.082

E 0.319 0.236 0.583 0.338 0.143 0.051 0.345 0.284

F 0.371 0.537 0.118 0.204 0.217 0.091 0.33 0.46
G 0.42 0.375 0.397 0.392 0.64 0.157 0.365 0.434
H
 File Name: 5000.dbf
Date: 17-05-2018 Time: 15:24:55

10 11 12 3TC
10 µM 1 µM 0.1 µM 0.01 µM
1.729 1.277 0.696 1.102 1.282 1.321
1.395 1.894 0.539 0.444 0.858 1.094
0.372 1.298 0.789 0.993 0.856 0.982
1.469 2.116 0.779 0.794 1.391 1.217
2.282 1.67 1.016 1.065 0.615 0.453
1.822 2.014 0.627 0.673 1.351 1.493

mean 0.741 0.845167 1.058833 1.093333

STDEV 0.164437 0.256647 0.323717 0.360356
10 11 12
relative to
UT 1 63.18033 72.06196 90.27995 93.22154
relative to
0.543 0.27 UT2 54.17327 61.78872 77.40953 79.93177
0.265 0.438
mean of
0.071 0.223 Untreated
relative to
0.22 0.475 mean of UT 58.33115 66.53109 83.35083 86.06665
0.643 0.325
0.349 0.331 untreated 3TC
c e l l v i a b i l i ty re l a t ve to u ntre a te d

0 µM 10 µM 1 µM 0.1 µM
100 58.33115 66.53109 83.35083

120
cell viability of HepG2.2.15 on day 14
100

80

60

40

20

0
u ntre ate d 3TC te n
 UT1 tenofovir UT2
0 µM 10 µM 1 µM 0.1 µM 0.01 µM 0 µM
0.938 0.521 1.11 1.141 1.186 1.007
1.27 0.316 0.813 0.959 1.13 1.456
1.456 0.187 1.359 0.167 0.301 1.075
0.808 0.112 1.35 1.234 1.249 1.641
1.185 0.383 1.306 1.355 1.639 1.345
1.38 0.524 1.486 1.459 1.473 1.683

1.172833 0.3405 1.237333 1.0525 1.163 1.367833

0.253354 0.169961 0.240964 0.466985 0.463624 0.282204

100 29.03226 105.4995 89.73995 99.16157 116.6264

85.74388 24.89338 90.45936 76.94651 85.02498 100

1.270333

100 26.80399 97.40226 82.85227 91.55077

3TC tenofovir
0.01 µM 10 µM 1 µM 0.1 µM 0.01 µM
86.06665 26.80399 97.40226 82.85227 91.55077

of HepG2.2.15 on day 14

3TC te no fovi r