Certicate of Analysis

Customer: CTV Date received: 04/25/2018

Invoice #: 18-04991 Date completed: 04/30/2018
Lab #: 17449 Report date: 05/21/2018
Analysis type: whole-sample NGS
Sample description: 10
Summary Table
Genus Species Subspecies/Strain % ID (avg.) % of Total Reads
Hippoglossus stenolepis - 99.69 11

Brief Synopsis: A total of 1,690 high-quality sequence reads were annotated with taxonomic information.
All annotations that met quality standards are for the sh species Hippoglossus stenolepis (Pacic
halibut).1,2 Annotations with <5% of total annotated sequences and/or not at least to the genus-level
were excluded from results table. 3

Denition of Terms in Report

Read(s) - An individual sequence of letters that has been created by a DNA sequencing technology and
represents the primary structure of a piece of DNA.

Annotation - The process of appending information from a reference sequence, such as source organsim, to
a query sequence of sucient similarity to the reference.

% ID - The percent similarity of the sample read when compared to a reference read.
% Total - The percent of total high-quality reads which were annotated with a specic taxon.

1
Sample matrix and homogeneity can inuence recovery of DNA and impact identications.
2
Other inclusions in
sample may be present due to external contamination or true adulteration.
3
Disclaimer: Exact Scientic Services is not responsible for the misuse or misinterpretation of the above information.