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Melatonin treatment tunes biochemical changes

and cellular architecture of endocrine pancreas in


streptozotocin induced diabetic rat

A Dissertation Submitted
In partial fulfillment for degree of
M.Sc. in Zoology

Submitted By
SHRISHTI SINGH
Under the supervision of
Dr. SEEMA RAI

DEPARTMENT OF ZOOLOGY
GURU GHASIDAS VISHWAVIDYALAYA
KONI, BILASPUR (C.G.) -495009
2017
Department of Zoology
Guru Ghasidas Vishwavidyalaya
(A Central University Established by the Central University act 2009, No. 25 of 2009)

CERTIFICATE

This is to certify that Miss. Shrishti Singh has worked on dissertation work
entitled “Melatonin treatment tunes biochemical changes and cellular
architecture of endocrine pancreas in streptozotocin induced diabetic rat” for
degree in M.Sc. (Zoology) under my supervision for three months and this work
has not been formed the basis for the award of any other similar title. It represents
entirely an independent work on the part of candidate.

Date:
Signature of Supervisor
Place: Bilaspur

Forwarded Head of the Department


Department of Zoology
Guru Ghasidas Vishwavidyalaya
(A Central University Established by the Central University act 2009, No. 25 of 2009)

DECLARATION

I, Shrishti singh, student of M.Sc. IV semester, Department of Zoology,


Guru Ghasidas Vishwavidyalaya, hereby declare that dissertation entitled
“Melatonin treatment tunes biochemical changes and cellular
architecture of endocrine pancreas in streptozotocin induced diabetic
rat” is a record of original research work done by me under the supervision
of Dr. Seema Rai, Associate professor, Department of Zoology, Guru
Ghasidas Vishwavidyalaya, Koni-Bilaspur 495009(C.G.).
The outcome of this work has not been submitted previously to any
other university for any examination by me.

Signature of the supervisor Signature of candidate

Date:
Place: Bilaspur
ACKNOWLEDGEMENT

I duly take this opportunity to express my deep sense of gratitude to project guide
Dr. Seema Rai for her valuable guidance and encouragement, rendered
throughout the course of investigation and in the preparation of this project work.
I express my sincere thanks to Dr. Monika Bhadauria, Head of the
Department of Zoology, Guru Ghasidas Vishwavidyalaya, for granting permission
and providing me with an environment to complete my project successfully within
the department.
I thank to my departmental teachers Dr. Santosh Singh, Dr. Sushant Kr
Verma, Dr. Manish Kr Tripathi, Dr. Vineet P. Singh, and Dr. Naresh
Chandra Agrawal for their valuable suggestions during the entire dissertation
work. I also extend my thanks to the non teaching staff of our department - Mr.
Bechan Paikra, Mr. Sushil Sharma, Mr. Balram Khairwar.
I am also thankful to our seniors Mr. Muddasir Basheer, Mr. Hindole
Ghosh and Mr. Younis Ahmad Hajam, for their great contribution to enrich my
knowledge and for their kind support and help during the experimental planning
and preparation of the project work. I also thank to all my friends and Swati,
Rajendra, Megha, Ankita, and Shivram, Yogesh and Ullash and my juniors
Monika, Neeraj, Nandani, and Amrish for their help.
I am deeply indebted to my parents Mr. Jugal Kishore Singh and Mrs.
Snehlata Singh and my siblings Kunwar Mritunjay Singh and Chitranshi
Nidhi Singh, who are always in all walks of my life and without their blessing it
was impossible for me to complete this work. Last but not the least; I thank God,
who has been with me in all paths of my life.

Place: Bilaspur
Date: SHRISHTI SINGH
DECLARATION

I, Shrishti Singh, hereby declare that the work presented in the project dissertation
entitled, “Melatonin treatment tunes biochemical changes and cellular architecture of
endocrine pancreas in streptozotocin induced diabetic rat ’’submitted as partial
fulfillment of M.Sc. (Zoology) was carried out at Department of Zoology, Guru Ghasidas
Vishwavidyalaya Bilaspur (C.G.).

The work was carried out by me under the supervision of Dr. Seema Rai
Department of Zoology. The work presented in this dissertation project is original and it
has not previously formed the basis for the award of any degree. It will remain
intellectual property of Department of Zoology, Guru Ghasidas Vishwavidyalaya
Bilaspur (C.G.).

Signature of Candidate Signature of Supervisor

Date:
Place: Bilaspur
CONTENTS

S. No. Title Page No.

1. Introduction 1-5

2. Review of literature 6-24

4. Materials and methods 25-29

5. Results 30-37

6. Discussion 38-42

7. Conclusion 43

8. References 44-47

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