HDL CHOLESTEROL KIT (PEG | CHOD - PAP method) rte getorinaon of HL Cholstoa Inseum or lasra (Forinvio Dagrose Use On) ‘Summary LUppreans arth pris which mainly wanspert fs in ‘hebeod sean, Toy canbe geupes ito oylomons, wry law density Spoprotins (VLDL). low density Tipopetcs (10) ard igh densty popes (HOL} Chylanicons and VIDL tanspart manly tgycdes, Bough VLDLs also anspor sare aout of choles LDLearasctlestelo he parperal tissues wherein be copes and inzeas th ik of atioscroc haat and periph vasa eae, Honea gh evel of LDL are aberogei. HDL varspets cist tem the spealtssueso te verfr exces, ence HDL nasa Drolecive eect The massuemen: of tal and HO ‘helestre and rgyerdes prove vail iremetin frnerisaseserentfoornay hart eens, Princpe When fe serum i roscted wen the Poythyane Gyo! carsined th repeating reagent alte VOL and LDL ‘re procptaled Te HDL remanenbe superar aris ‘hor assayec ab a saree fr chlstarcl usng the Chelsioro(CHODPAP) cago. Normalrefrence values Prognostally Standard Risk favourable RiskLevelIndzator HOLOWL mass 55 5-85.35 (mg) fomaee 7655-655 UDLcre males st > Tue ae, } <1 tso-ton > 100 Wala, nase 3828-69 <5 HOLCro, finales 3313148 ees 1s rermended hat each bere extabah is own era rangerepreseringls pate oauatn. Contents 15m Lis Engme Reagent 1 oni 12: Emme Reagort2 16m 13: reiting Rapport 25m HDL Chlstral Slandard (25 mala) Sm Storage stabity Contents ae ssl 28°C a te expiry menions on tele. Reagent Preparation Reagents ar ready ue, Working reagent: Pour tho cont of 1 bole of 2 (Eyre Reagent 2) tbl of Lt (Enz Reagort 1) Teswocingreagetisabaoralios. 6 eaks wren LT ‘Soret at 28°C, Upon sage the working reagent nay Getto asighipine clo owever Sis does at alee armanes othe eagon: ‘Amatl fr exiey ab much f working reagent nay bemace sana when cased by mixing taper pars of Li (Enayme Reagent and garot L (Enzyme Reagent 2}Aoratly 08 of 1 and 0.2m) of 2 may also ba Used insta a ro he working reagent recy ing eassay, ‘Sample matral ‘Seun, ETA plasma, Chalet! & HDL Cholesaa are roportediobe salen sri for days hon saad 2° (CThesarsle stad prefrabiybeot 1a ahourstasig, Procedure Wavelet 50am (9 48 rm) Geen Tongeraure wert Usnah ton Precipitation of VLDL LOL: peters ceanay ete racing Reaper 03) Sempe Ti 1m: Micwel an neste at orS min. Cette at 2500 - orpmlo bla aces” peasant. ‘Cholesterol Assay it in ean dy ‘Standard, and Test) lubes lated as Bark (2), ‘Aiton . os 7 Sequence a ae Wang reagent to 1040 Distledwater os HL Stender) 005 Supamatant™ 008 Nix wel and abate at 37 fo Sin ora. 25°) or {Smin. Measure th absorbance of to Standard (Ads), and Test Sarl (Abs T gate lank win 60, ‘ony Total Cholestos be estarined use eny 0.01 rl of DW/Chel SeSenple ares in the cet! sey Caletations pos HOLCroesarclinngd = x 2x2 boss (Wve 2s he dlton factor du tb dpretnizaton sp) Calelation of LOL Cholesterol mg) (Freéewads Ferme) gy = {iltsion) — | —— ] — crsFrecowaidsFomua i eabl proved ts {No homisors are poset. tis fst sompl 2 Thgyeaie vals ae below 400 1 Tpe ll yperipoprtiorais absent Linearity “his procedures incr up 180 raidlof HL Chalet Irae exceed ti int, data te sum wih raral seine (Nat 0.9%) and ropat to assay. Calle ho vaususngthe proper dluonfator Note The spemaant shouldbe clear # is hazy or Saud, the saple shoud be died 1+ wih moral eae (§aC10.9%) ad the precptton sep shaud ba repeat (Resuisx2) Aaleoanian’s such as four, ork serum shouldbe vd, yan hao References “dr .('968)Ann. Cin Bochon 6:28 ‘Mla, etal (874 Ci, Chem. 20:¢70 Fege, HM, (1872)Ann Ci. Bite 10:78 ilo Fea (861 Cin. Chem. 27375 Demasto; PNM, eal (1880) Cin Chem 26:1775 System Parameters LT Reaction End Pont Wavelength 505m Zero Seting Roagert Blak Ineub. Temp, S7CIRT, Ineub: Time Sri. 15min DeltyTime: = ReadTime : Novofread : = Interval Sample Vol 005ml ReagentVol, 100m Standard: 2emgidlx2 Factor 2 React Slope: lnceasing Lineanty ‘sang Unis ng ‘Gana Arteris Do Rago Bagh, Bamboim Gorpox 20. GOA. 403202, NDIA