NAME OF UNIVERSITY/SCHOOL/COLLEGE

LOGO Location
College of Medical Technology/Medical Laboratory Science
Learning Plan/Instructor’s Guidein Microbiology ( Bacteriology 1)
 VISION (University/College) Vision of the College/School/Department of Medical Technology/Medical
Laboratory Science
MISSION (University/College)

Mission of the College/School/Department of Medical Technology/Medical

INSTITUTIONAL CORE VALUES Laboratory Science

Institutional Intended Learning Outcomes (ILOs)

Student Outcomes (SO) or SLO
A _________ must be able to: (Sample Only) SO # 1.
demonstrate technical competence in the performance of clinical laboratory tests in
ILO 1:exhibit proficiency in the practice of his/her profession( Proficient
aid of diagnosis, treatment and management of diseases vis-à-vis biosafety and waste
and Trained Expert) management;

Description: A proficient and trained expert is one who can solve and deal Performance Indicators (PI)
with problems in their respective professions
The graduates shall be able to:
ILO 2 : demonstrate excellent leadership skills ( Excellent Leader)
 collect, handle and process biological specimens properly and safely;
Description: An excellent leader who can establish good working relationship
in achieving professional goals
 perform laboratory testing accurately through the use of appropriate techniques,
skills and technology;
ILO 3:manifest ethical standards and behavior( Ethical practitioner)
 analyze and interpret laboratory test data;
Description: An ethical practitioner who can implement standard procedures
or norms in the practice of their profession
 monitor testing procedures, equipment and professional/ technical competency
using quality assurance methodologies;
ILO 4:generate new knowledge through scientific inquiries manifesting
responsibility ( Responsible Professional, Lifelong Knower and Doer )
 operate instruments properly and perform appropriate preventive and corrective
Description: A responsible individual is one who can exemplify dedication in maintenance;
the practice of their profession . He/she is responsible to self, community and
country they serve  adhere to all laboratory safety rules and regulations;

 use computers and laboratory software competently;

 discuss appropriate and novel technology for medical technology/medical

laboratory science application;

 carry out the evaluation of new procedures and instruments; and

 UNCIACOLLEGES, INC.
Circumferential Road, Antipolo City
College of Medical Technology/Medical Laboratory Science

Learning Plan /Instructor’s Guide

In Microbiology( Bacteriology 1)

Course Title: Microbiology 1 (Bacteriology)

Course Description:

The course covers the morphology and physiology of bacteria, rickettsiae and other significant atypical bacteria and their role in infection and immunity. Emphasis is made on their isolation
and identification and susceptibility testing as an aid in laboratory diagnosis.

Course Credit: 5 units (3 units lecture, 2 unit laboratory)

Contact Hours: 3 hours lecture, 6 hours laboratory per week (54 hours lecture and 108 hours laboratory per semester)

Prerequisites: Biochemistry, Human Anatomy and Physiology

Placement: Third Year, 1st Semester

Course Learning Outcomes :

At the end of this course, the student is able to:
1. Identify bacteria according to standard laboratory techniques: microscopic and staining; growth and biochemical characteristics; and antigen typing.
2. Perform appropriate antimicrobial susceptibility testing
3. Operate common instruments used in Bacteriology
4. Participate in quality assurance/quality control program in Microbiology
5. Understand the duties and responsibilities of medical technologists in the clinical setting
6. Perform proper specimen collection, processing, transport and waste disposal
7. Apply concepts in controlling the growth and spread of pathogenic bacteria

References: Please write the latest edition and year of publication

1. Alcamo, Edward I. Fundamentals of Microbiology 6th ed. Boston: Jones and Barlett Publishers, 2001.
2. Bartlet, Margaret A. Diagnostic Bacteriology: A Study Guide. F.A. Davis Co. Philadelphia, USA, 2001.
3. Black, Jacquelyn. Microbiology: Principles and Explorations 7th ed. USA: Wiley, 2008.
4. Brooks, Geoff. Jawetz, Melnick&Adelberg’s Medical Microbiology 24th ed. Boston: McGraw Hill, 2007.
5. Burton, Gwendolyn and Paul Engelkirk. Burtn’s Microbiology for the Health Sciences 8th ed. USA: Lippincott Williams and Wilkins, 2007.
6. Forbes, Betty, Daniel Salm and Alice Weissfield. Bailey & Scott’s Diagnostic Microbiology 12th ed. USA: Mosby, 2007.
7. Funke, Berdell. Microbiology: An Introduction. USA: Addison Wesley Publishing Co., 2007.
8. Gladwin, Mark and Bill Trattler. Clinical Microbiology Made Ridiculously Simple 4th ed. USA: MedMaster, Inc., 2007.
9. Hawkey, Peter and Dierdre Lewis. Medical Bacteriology: A Practical Approach 2nd ed. USA: Oxford University Press, 2004.
10. McClatchey, Kenneth. Clinical Laboratory Medicine 2nd ed. USA: Lippincott Williams and Wilkins, 2002.
 11. Mcpherson, Richard A. and Matthew R. Pincus. Henry’s Clinical Diagnosis and Management by Laboratory Methods 21st ed. Philadelphia: Elsevier Inc., 2007.
12. Murray, Patrick R. et. al. Medical Microbiology 5th ed. USA: Mosby, 2005.
13. Pommerville, Jeffrey. Alcamo’s Fundamentals of Microbiology 8th ed. USA: Jones and Bartlett Publishers, 2006.
14. Presscott, L., J. Harley and D. Klein. Microbiology 6th ed. USA: McGraw-Hill Science, 2004.
15. Tortora, G., B. Funke and C. Case. Microbiology: An Introduction 9th ed. USA: Benjamin Cummings, 2006.
16. Vandepitte J., J. Verhaegen and K. Engbaek. Basic Laboratory Procedures in Clinical Bacteriology 2nd ed. World Health Organization, 2003.

Electronic References:
1. http://connection.lwww.com/go/burton/7e
2. http://medic.med.uth.tmc.edu/path/00001450.htm
3. http://www.kensbiorefs.com/Microbio.html
4. http://www.microbes.info
5. http://www.splammo.net/JLbactsite.html

Unit Learning Outcomes ( ULOs) Teaching Learning Activities Assessment Tasks Resources
Content T.A. Lecture Laboratory Values Deployed Assessm Tools
ent
At the end of this unit, the student is I. Introduction to Microbiology Didactic Demonstration Adherence to rules MCQ Answer Textbooks
able to: Lecture: 2 Method Return Responsibility TFQ Keys
1. Define terms 1. Definition of terms: hrs demonstration Accountability MTQ E-books
2. Enumerate the development of a. Bacteriology, bacteria, Lecture Cooperation
Bacteriology as a science with pathogenecity, virulence, etc. Or Blended Engaged Practical Rubrics Prepared
emphasis on person/scientists and 2. Historical development Lecture Method of Teamwo exam. on PPT
their contributions 3. Divisions of Microbiology /Discussion Learning Laborat
3. Explain the divisions of ory Laboratory Manual
Microbiology Laboratory: Performance of Perform
4. Discuss laboratory safety and 1. Expectations/requirements of 6 tests ance
 general considerations regarding Microbiology Laboratory hrs
proper handling, processing, 2. Rules to follow in handling,
storage of specimens and proper processing, disposal of
waste disposal specimens/waste in microbiology
5. Discuss the roles of a medical laboratory
technologist in a microbiology 3. Barrier precautions
laboratory 4. Proper use of different apparatus,
materials and equipment:
5. Review use and care of
microscope
At the end of this unit, the student is II. Laboratory Safety & Infection Inductive Engaged Honesty MCQ Answer
able to: Control 3hrs Teaching Method of Commitment MTQ Keys Textbooks
1. Define terms Lecture: ( Specific to Learning Adherence to rules
2. Discuss concepts, principles & 1. Sterilization General) on Responsibility Identificat Rubrics E-books
significance of infection control & a. Physical (Heat) Case Analysis Actual Accountability ion of on
laboratory safety - Autoclave performance Cooperation Equipmen Laborat Prepared
3. Discuss various methods of - Direct heat Computer- Return Teamwork t ory Test PPT
sterilization & disinfections - Oven Assisted demonstration
:temperature , time, - Incineration Methodology Laboratory Manual
principles/mechanism involved, - Pasteurization Discussion of Case
when to use; advantages & - Boiling Blend Method test results Analysis
disadvantages b. Filtration of Teaching Making
4. Differentiates the different 2. Disinfection Lecture / conclusions & Problem
biosafety cabinets a. Chemicals discussion guidelines Solving
5. Explain the significance of b. Alcohol
pathogenic and virulence factors in c. Chlorine
the development of infections d. Others
6. Perform correctly sterilization & 3. Antimicrobial agents
disinfection practices 4. Antimicrobial Susceptibility testing
7. Explain the concepts behind a. Automated
Antimicrobial Susceptibility testing b. Diffusion
8. Differentiate between the different c. Dilution
types of antimicrobial susceptibility 5. Use of bio-safety cabinets
testing 6. Barrier precautions
9. Discuss significance advantages 7. Control of nosocomial infections
and disadvantages of each 6
method of sensitivity test Laboratory: hrs
10. Interpret test results 1. Sterilization:
11. Explain the concepts of a. Autoclaving
nosocomial infections b. Direct heating
12. Outline the roles of med tech in c. Filtration
prevention & control of nosocomial 2. Cleaning of working area with
infections Clorox and other chemicals
3. Proper handwashing techniques
4. Lab exercises on the effects of
chemicals & temperature on
bacteria
5. Proper working attire in a
Bacteriology laboratory
At the end of this unit, the student is III. Bacterial Structure and Function, Inquiry Engaged Honesty MCQ Answer
able to: Growth and Nutrition 3hrs Method of Method of Commitment Key
1. Describe bacteria according to: Lecture: Teaching Learning Adherence to rules Case Textbooks
physiology, replication, 1. Characteristics of Bacterial Responsibility study Rubrics
morphology according to: Or Actual Accountability on E-books
2. Identify the growth requirements a. Physiology: Structure, Investigating performance Cooperation Laborat
for each particular group of Function Puzzling Return Teamwork Problem ory Prepared
bacteria b. Bacterial morphology Situation demonstration solving Perform PPT
3. Discuss the growth curve with c. Classification ance
emphasis on the events d. Replication Computer- Discussion of Practical Laboratory Manual
happening per stage e. Growth Requirements aided lecture / test results examinati Rubrics
4. Discuss proper specimen - Physical discussion Making on on Case
collection, labelling, transport, - Nutritional conclusions & Study
storage, processing, and waste 2. Bacterial Growth Curve guidelines
disposal 3. Bacterial Identification Multi-tasking Rubrics
5. Perform correctly specimen a. Microscopy: Morphology on
collection: b. Cultural/Colony Problem
6. Perform correctly hanging drop Characteristics Solving
and wet mount techniques c. Staining characteristics
7. Describe the results of hanging d. Biochemical
drop and wet mounts e. Serological: Antigen testing
8. Discuss concepts and principles of f. Molecular Techniques
the laboratory techniques used for 4. Quality Assurance-Quality Control 10
identification. hrs
9. Prepare a general schema for Laboratory:
identification of bacteria in the 1. Acceptable specimens for bacterial
laboratory identification
10. Describe microscopic morphology a. Swabs
and staining reactions of bacteria. b. Blood
11. Identify correctly bacterial c. Urine
morphology and staining reactions. d. Stool
12. Perform laboratory exercises e. Tissue
correctly f. Aspirates
13. Write a report based on accepted g. Others
standard format 2. Specimen collection, handling,
transport, processing and
preservation
3. Hanging drop method and wet
mounts
4. Role of Microscopy in Bacterial
identification
a. Bright field
b. Phase contrast
c. Electron
d. Dark-field
e. Fluorescent
5. Preparation of Bacterial Smear for
staining from
a. Directly from specimen (throat
swab or any swab)
b. Colonies growing in culture
 media
6. Staining Techniques
a. Simple stain
b. Differential stain
- Gram stain
- Acid fast stain
c. Special stains
d. others
7. laboratory Safety
8. Laboratory Waste Management
At the end of this unit, the student is IV. Bacterial Genetics Inquiry MCQ Answer Textbooks
able to: Lecture: 2hrs Method of Honesty Key
1. Discuss the concepts of bacterial 1. Basic Concepts Teaching on Commitment Case E-books
genetics and its importance in the a. Bacterial DNA and RNA Case Study Adherence to rules study Rubrics
study of bacteria b. Plasmids & Microbial Responsibility on Lab. Prepared
2. Appreciate the role of medical Resistance Or Accountability Test PPT
technologists in the control and c. DNA exchange in Bacteria Investigating Cooperation Problem
prevention of microbial resistance - Transformation Puzzling Teamwork solving Rubrics Laboratory Manual
- Transduction Situation on Case
- Conjugation Study
Computer-
aided lecture / Rubrics
discussion on
Problem
Solving
At the end of this unit, the student is V. Pathogenesis and Epidemiology Inquiry Honesty MCQ Answer Textbooks
able to: Lecture: 2hrs Method of Commitment Key
1. Basic Concepts Teaching on Adherence to rules Case E-books
1. Discuss the modes of transmission 2. Mode of Transmission Case study Responsibility study Rubrics
of bacterial infections 3. Factors associated with Or Accountability on Lab. Prepared
2. Discuss the factors that contribute pathogenicity and virulence Investigating Cooperation Test PPT
to pathogenicity and virulence a. Toxins Puzzling Teamwork Problem
b. Enzymes Situation solving Rubrics Laboratory Manual
c. Capsules on Case
d. Others Computer- Study
assited
lecture / Rubrics
discussion on
Problem
Solving
At the end of this unit, the student is VI. Normal Flora Large group Engaged Adherence to rules
able to: Lecture: 2hrs discussion Method of Responsibility Textbooks
1. Define terms 1. Basic Concepts Power point Learning Accountability
2. Identify body sites with normal 2. Body Sites with Normal Flora presentation Cooperation E-books
flora 3. Predominant flora Demonstration
3. Discuss the predominant normal 4. Beneficial and harmful effects of & return Prepared
flora in each body site normal flora demonstration PPT
4. Explain the beneficial and harmful Performance of
effects of normal flora Laboratory: 6 tests Laboratory Manual
1. Isolation of normal flora hrs
 2. Laboratory safety
3. Laboratory waste management

VII.
At the end of this unit, the student is VIII. Micrococcaceae and Didactic Performance of MCQ Answer
able to: Streptococcaeae Method Tests Honesty Key
1. Describe Micrococcaceae& 1. Micrococcaceae Commitment Case Textbooks
Streptococcus in terms of their a. staphylococcus Lecture with Demo and Adherence to rules study Rubrics
general properties and b. micrococcus demonstration Return Responsibility on Lab. E-books
pathogenesis 2. Stretococcaceae Multimedia demonstration Accountability Test
2. Discuss the identification a. Streptococcus presentation Cooperation Problem Prepared
characteristics of Micrococcaceae b. entererococcus Teamwork solving Rubrics PPT
in terms of morphology, culture 3.5 Concern for others on Case
media, colony characteristics, Lecture: hrs Interactive & the environment Board Study Laboratory Manual
biochemical test, and serological 1. General characteristics Discussion Work
test 2. Pathogenesis & spectrum of Rubrics
3. Explain the principles/concepts of disease Practical on
different identification techniques 3. Laboratory identification exam. Problem
used a. Specimen used for isolation Solving
4. Differentiate pathogenic from non- b. Morphology & Staining
pathogenic members of the genus Reaction
5. Enumerate the appropriate c. Culture media and colony
specimen submitted in the characteristics
laboratory for isolation d. Biochemical tests
6. Prepare a schematic diagram for e. Antigen typing/serology
identification 4. Prevention and control 6
7. Classify culture media according hrs
to: indications for use, contents, Laboratory:
purpose, etc. 1. Culture media
8. Prepare different culture media a. Classification
9. Perform quality control of culture b. Composition
media before use c. Preparation
10. Identify Staphylococcus, d. Storage
Micrococcus & Streptococcus from e. Quality assurance/control
specimens - Spore strip test
- Sterility test
2. Preparation of culture media
a. Nutrient Agar
b. Blood Agar Plate
c. Chocolate Agar Plate
3. Identification of Staphylococcus
and Streptococcus from
specimens, prepared slides and
visual aids
4. Laboratory safety
5. Laboratory waste management
At the end of this unit, the student is IX. Neisseriaceae Didactic Performance of MCQ Answer
able to: 1. Neisseriaceae Method Tests Honesty Key
1. Describe the members of a. Pathogenic Neisseria Demo and Commitment Case
Neisseriaceae in terms of their b. Non-pathogenic Neisseria Lecture with Adherence to rules study Rubrics
 general properties and c. Branhamellacatarrhalis demonstration Return Responsibility on Lab. Textbooks
pathogenesis Multimedia demonstration Accountability Test
2. Discuss the identification Lecture: 3.5 presentation Cooperation Problem E-books
characteristics of Neisseriaceae in 1. General characteristics hrs Teamwork solving Answer
terms of morphology, culture 2. Pathogenesis & spectrum of Concern for others Keys Prepared
media, colony characteristics, disease Interactive & the environment Board PPT
biochemical test, and serological 3. Laboratory identification Discussion Work
test a. Specimen used for isolation Laboratory Manual
3. Explain the principles/concepts of b. Morphology and staining Honesty Practical
different identification techniques reaction Inquiry Commitment exam.
used c. Culture media and colony Method of Adherence to rules
4. Different pathogenic from non- characteristics Teaching Responsibility
pathogenic members of the genus d. Biochemical tests Accountability
5. Enumerate the appropriate e. Antigen typing/serology Or Cooperation
specimen submitted in the 4. Prevention and control Investigating Teamwork
laboratory isolation and 6 Puzzling Concern for others
identification Laboratory: hrs Situation & the environment
6. Prepare a schematic diagram for 1. Identification techniques for:
identification a. Pathogenic Neisseria
7. Perform correctly inoculation b. Non-pathogenic Neisseria
techniques and tests c. Branhamellacatarrhalis
8. Summarize, based on results of 2. Identification of species using
identification techniques, bacterial prepared slides and visual aids
flora in a human person and 3. Laboratory safety
environment 4. Laboratory waste management

At the end of this unit, the student is X. Enterobacteriaceae Didactic Engaged MCQ Answer
able to: 1. True Intestinal Pathogens Method of Honesty TFQ Keys Textbooks
1. Discuss the process of a. Salmonella Learning Commitment MTQ
identification of b. Shigella Lecture with Adherence to rules Rubrics E-books
Enterobacteriaceae in terms of c. Yersinia demonstration Performance of Responsibility Practical on Lab.
morphology, culture media, colony d. Escherichia Multimedia Tests Accountability exam. Test Prepared
characteristics, biochemical test, 2. Opportunistic pathogens 4.5 presentation Return Cooperation PPT
serological test a. Escherichia hrs demonstration Teamwork Performa Rubrics
2. Explain the principles/concepts of b. Citrobacter Interactive Concern for others nce on Laboratory Manual
different identification techniques c. Proteus Discussion & the environment evaluatio Return
used d. Edwardsiella n Demo
3. Different pathogenic from non- e. Morganella
pathogenic members of the genus f. Hafnia Inquiry Rubrics
4. State the pathogenic and virulence g. Providencia Method of for
factors present in bacteria h. Enterobacter Teaching Perform
belonging to the group and i. Serratia ance
disease caused by them j. Klebsiella Or evaluati
5. Enumerate the appropriate k. Others Investigating on
specimen submitted in the Puzzling
laboratory isolation and Lecture: 9 Situation
identification 1. General characteristics hrs
6. Prepare a schematic diagram for 2. Pathogenesis & spectrum of
identification disease
7. Properly inoculate specimen on 3. Laboratory identification
 culture media f. Specimen used for isolation
8. Summarize, based on results of g. Morphology and staining
identification techniques, bacterial reaction
flora in a human person & h. Culture media and colony
environment. characteristics
9. Perform identification techniques i. Biochemical tests
10. Identify correctly the isolate j. Antigen typing/serology
11. Write result based on the accepted 4. Prevention and control
standard format.
Laboratory:
1. Identification techniques for
enterobacteriaceae:
morphology, colony
morphology, biochemical
tests

2. Laboratory safety
3. Laboratory waste management
At the end of this unit, the student is XI. Non-enteric Gastrointestinal Didactic Engaged Honesty MCQ Answer
able to: Pathogens Method Method of Commitment TFQ Keys Textbooks
1. Discuss the process of 1. Vibrio Learning Adherence to rules MTQ
identification of the 2. Campylobacter Responsibility Rubrics E-books
members of the non-enteric 3. Helicobacter Lecture with Performance of Accountability Board on Lab.
gastrointestinal pathogens 4. Aeromonas demonstration Tests Cooperation work Test Prepared
in terms of morphology, 5. Pleisomonas Multimedia Teamwork PPT
culture media, colony 6. Others presentation Concern for others Case Rubrics
characteristics, biochemical Discussion Return & the environment study on Laboratory Manual
test (plus positive & Lecture: 3.5 demonstration Return
negative results), serological 1. General characteristics hrs Inquiry Demo
test 2. Pathogenesis & spectrum of Method of
2. Explain the disease Teaching
principles/concepts of 3. Laboratory identification
different identification a. Specimen used for isolation Or
techniques used b. Morphology and staining Investigating
3. Different pathogenic from reaction Puzzling
non-pathogenic members of c. Culture media and colony Situation
the genus characteristics
4. Enumerate the appropriate d. Biochemical tests
specimen submitted in the e. Antigen typing/serology
laboratory isolation and 4. Prevention and control 6
identification hrs
5. Construct a schematic Laboratory:
diagram for identification 1. Isolation and Identification of
6. Prepare a schematic members of non-enteric
diagram for identification gastrointestinal pathogens
7. Properly inoculate specimen 2. Identification of species using
on culture media prepared slides and visual aids
8. Summarize, based on 3. Laboratory safety
results of identification 4. Laboratory waste management
techniques, bacterial flora in
 a human person &
environment.
9. Perform identification
techniques
10. Identify correctly the isolate
11. Write result based on the
accepted standard form

At the end of this unit, the student is XII. Non-fermentative Gram Negative Didactic Engaged MCQ Answer Textbooks
able to: Bacilli and Aerobic Gram Positive Method of Method of Honesty TFQ Keys
1. Discuss the process of Bacilli Teaching Learning Commitment MTQ E-books
identification of the non- 1. Non-fermentative Gram Negative Adherence to rules Rubrics
fermentative group in terms of Bacilli Lecture with Performance of Responsibility Board on Lab. Prepared
morphology, culture media, colony a. Pseudomonas demonstration Tests Accountability work Test PPT
characteristics, biochemical test b. Acinetobacter Multimedia Return Cooperation
(plus positive & negative results), c. Flavobacterium presentation demonstration Teamwork Case Rubrics Laboratory Manual
serological test d. Burkholderia Concern for others study on
2. Explain the principles/concepts of e. Alcaligenes Interactive & the environment Return
different identification techniques f. Achromobacter Discussion Practical Demo
used g. Stenotrophomonas exam.
3. Different pathogenic from non- h. Others
pathogenic members of the genus 2. Aerobic Gram Positive Bacilli
4. Enumerate the appropriate a. Bacillus
specimen submitted in the b. Listeria
laboratory isolation and c. Corynebacterium
identification d. Erysipelothrix
5. Prepare a schematic diagram for e. Nocardia
identification f. Streptomyces
6. Perform correctly inoculation g. Others
techniques and tests
Lecture: 3.5
1. General characteristics hrs
2. Pathogenesis & spectrum of
disease
3. Laboratory identification
a. Specimen used for isolation
b. Morphology and staining
reaction
c. Culture media and colony
characteristics
d. Biochemical tests
e. Antigen typing/serology
4. Prevention and control 6
hrs
Laboratory:
1. Isolation and Identification of
members of non-fermentative gram
negative bacilli and aerobic gram
positive bacilli
2. Identification of species using
 prepared slides and visual aids
3. Laboratory safety
4. Laboratory waste management
At the end of this unit, the student is XIII. Small, Pleomorphic Gram Didactic Engaged Honesty MCQ Answer Textbooks
able to: Negative Bacilli Method of Commitment TFQ Keys
1. Discuss the process of 1. Hemophilus Lecture with Learning Adherence to rules MTQ E-books
identification of small pleomorphic 2. Bordetella demonstration Responsibility Rubrics
gram negative bacilli and the 3. Brucella Multimedia Performance of Accountability on Lab. Prepared
aerobic gram positive bacilli in 4. Francisella presentation Tests Cooperation Test PPT
terms of morphology, culture 5. Gardnerella Discussion Teamwork
media, colony characteristics, 6. Legionella Demo and Concern for others Rubrics Laboratory Manual
biochemical test (plus positive & 7. Others Return & the environment on
negative results), serological test demonstration Return
2. Explain the principles/concepts of Lecture: 3.5 Demo
different identification techniques 1. General characteristics hrs
used 2. Pathogenesis & spectrum of
3. Different pathogenic from non- disease
pathogenic members of the genus 3. Laboratory identification
4. State the pathogenic & virulence a. Specimen used for isolation
factors present in bacteria b. Morphology and staining
belonging to the group and reaction
disease caused by them c. Culture media and colony
5. Determine the appropriateness of characteristics
the different specimens submitted d. Biochemical tests
in the laboratory for isolation & e. Antigen typing/serology
identification 4. Prevention and control 6
6. Prepare a schematic diagram for hrs
identification Laboratory:
7. To distinguish the species of the 1. Isolation and Identification of
aerobic gram positive bacilli from members of small, pleomorphic
other bacteria gram negative bacilli
8. Identify members from submitted 2. Identification of species using
sample prepared slides and visual aids
3. Laboratory safety
4. Laboratory waste management
At the end of this unit, the student is XIV. Mycobacterium Group Didactic Engaged Honesty MCQ Answer
able to: 1. Mycobacterium tuberculosis Method of Method of Commitment TFQ Keys Textbooks
1. Discuss the process of complex Teaching Learning Adherence to rules MTQ
identification of Mycobacteria in 2. Non-tuberculosis Mycobacteria Responsibility Rubrics E-books
terms of morphology, culture 3. Mycobacterium leprae Lecture with Performance of Accountability Board on Lab.
media, colony characteristics, 3.5 demonstration Tests Cooperation work Test Prepared
biochemical test (plus positive & Lecture: hrs Multimedia Teamwork PPT
negative results), serological test 1. General characteristics presentation Demo and Concern for others Case Rubrics
2. Explain the principles/concepts of 2. Pathogenesis & spectrum of Return & the environment study on Laboratory Manual
different identification techniques disease Interactive demonstration Return
used 3. Laboratory identification Discussion Demo
3. Different pathogenic from non- a. Specimen used for isolation
pathogenic members of the genus b. Morphology and staining
4. State the pathogenic & virulence reaction
factors present in bacteria c. Culture media and colony
 belonging to the group and characteristics
disease caused by them d. Biochemical tests
5. Determine the appropriateness of e. Antigen typing/serology
the different specimens submitted 4. Prevention and control 6
in the laboratory for isolation & a. TB-DOTS hrs
identification
6. Prepare a schematic diagram for Laboratory:
identification 1. Isolation and Identification of
7. To distinguish the species members of Mycobacteria
8. Identify members from the gram a. Staining
positive bacilli from other bacteria - Acid Fast Staining
submitted sample - Other Staining
Techniques
2. Identification of species using
prepared slides and visual aids
3. Laboratory safety
4. Laboratory waste management
At the end of this unit, the student is XV. Anaerobic Bacteria Didactic Engaged Honesty MCQ Answer Textbooks
able to: 1. Clostridium Method of Method of Commitment TFQ Keys
1. Enumerate the characteristics of 2. Bacteroides Teaching Learning Adherence to rules MTQ E-books
anaerobic bacteria in terms of 3. Actinomyces Responsibility Rubrics
metabolism/physiology 4. Fusobacterium Lecture with Performance of Accountability Board on Lab. Prepared
2. Explain precautions of the 5. Peptostreptococcus demonstration Tests Cooperation work Test PPT
collection, processing, handling, 6. Porphyromonas Multimedia Teamwork
disposal of specimen 7. Bifidobacterium presentation Demo and Concern for others Case Rubrics Laboratory Manual
3. Discuss the clinically significance 8. Lactobacillus Return & the environment study on Case
anaerobic in terms of morphology, 9. Veilonella Interactive demonstration Study
isolation techniques, biochemical 10. Megasphaera Discussion
tests 11. Acidomicocus Rubrics
4. Perform the set-up of anaerobic 12. Others on
bacteriology Board
5. Discuss the importance of the Lecture: 3.5 Work
components in the set-up 1. General characteristics hrs
2. Pathogenesis & spectrum of
disease
3. Laboratory identification
a. Specimen collection &
processing
b. Anaerobic set-up and culture
media
4. Prevention and control 6
hrs
Laboratory:
1. Isolation and Identification of
members of anaerobic bacteria
a. Anaerobic culture
b. Anaerobic set-up
c. Microscopic identification
2. Identification of species using
prepared slides and visual aids
 3. Laboratory safety
4. Laboratory waste management
At the end of this unit, the student is XVI. Spirochetes Didactic Engaged Honesty MCQ Answer
able to: 1. Treponema Method of Method of Commitment TFQ Keys Textbooks
1. Discuss the process of 2. Borrelia Teaching Learning Adherence to rules MTQ
identification of medically 3. Leptospira Responsibility Rubrics E-books
important Spirochetes Accountability Board on Lab.
2. Correlate appropriate specimen Lecture: 3 Lecture with Performance of Cooperation work Test Prepared
with isolates 1. General characteristics hrs demonstration Tests Teamwork PPT
3. Characterize the organisms 2. Pathogenesis & spectrum of Multimedia Concern for others Case Rubrics
according to morphology and disease presentation Return & the environment study on Case Laboratory Manual
pathophysiology 3. Laboratory identification demonstration Study
4. Perform correctly staining 4. Prevention and control Interactive
techniques for Spirochetes Discussion Rubrics
5. Describe Spirochetes observed Laboratory: 3 on
under the microscope 1. Staining techniques for hrs Board
Spirochetes Work
2. Identification of species using
prepared slides and visual aids
3. Laboratory safety
4. Laboratory waste management

At the end of this unit, the student is XVII. Unique Bacteria/Atypical Didactic Engaged Honesty MCQ Answer Textbooks
able to: Bacteria Method of Method of Commitment TFQ Keys
1. Discuss the process of 1. Rickettsiaceae Teaching Learning Adherence to rules MTQ E-books
identification of unique bacteria a. Rickettsia Responsibility Rubrics
2. Explain the pathogenesis and b. Ehrlichia Accountability Board on Lab. Prepared
spectrum of disease caused by c. Orientia Lecture with Performance of Cooperation work Test PPT
unique bacteria 2. Mycoplasmataceae demonstration Tests Teamwork
3. Discuss the mode transmission of a. Mycoplasma Multimedia Return Concern for others Case Rubrics Laboratory Manual
the microorganisms b. Ureaplasma presentation demonstration & the environment study on Case
4. Explain the principles behind the 3. Chlamydiaceae Interactive Study
tests employed to identify unique a. Chlamydia Discussion
bacteria b. Chlamydophila Rubrics
5. Describe the members of the 3 on
group as observed under the Lecture: hrs Board
microscope 1. General characteristics Work
2. Pathogenesis & spectrum of
disease
3. Laboratory identification
4. Prevention and control

At the end of this unit, the student is XVIII. Miscellaneous Bacteria Didactic Engaged Honesty MCQ Answer
able to: 1. Eikinella Method of Method of Commitment TFQ Keys Textbooks
1. Discuss the process of 2. Kingella Teaching Learning Adherence to rules MTQ
 identification of miscellaneous 3. Streptobacillus Responsibility Rubrics E-books
bacteria 4. Bartonella Accountability Board on Lab.
2. Explain the pathogenesis and 5. Spirillum Lecture with Performance of Cooperation work Test Prepared
spectrum of disease caused by 6. Others demonstration Tests Teamwork PPT
miscellaneous bacteria Multimedia Return Concern for others Case Rubrics
3. Discuss the mode transmission of Lecture: 3 presentation demonstration & the environment study on Case Laboratory Manual
the microorganisms 1. General characteristics hrs Study
4. Explain the principles behind the 2. Pathogenesis & spectrum of Interactive
tests employed to identify disease Discussion Rubrics
miscellaneous bacteria 3. Laboratory identification on
4. Prevention and control Board
Work

At the end of this unit, the student is XIX. Applied Bacteriology Didactic Engaged MCQ Answer
able to: Lecture: 3 Method of Method of Honesty TFQ Keys
1. Discuss the process of 1. Applied Bacteriology hrs Teaching Learning Commitment MTQ Textbooks
water, food and a. Water Bacteriology Adherence to rules Rubrics
environmental bacteriology. b. Food Bacteriology Responsibility Board on Lab. E-books
c. Milk Bacteriology Lecture with Performance of Accountability work Test
2. Prepare media for the finale d. Environmental Bacteriology demonstration Tests Cooperation Prepared
examination Multimedia Return Teamwork Case Rubrics PPT
3. Perform correctly the procedures Laboratory: presentation demonstration Concern for others study on Case
in water, food and milk analysis 1. Laboratory identification of 9 & the environment Study Laboratory Manual
4. Interpret the results of the analysis unknown bacteria as practical hrs Interactive
examination Discussion Rubrics
2. Laboratory safety on
3. Laboratory waste management Board
Work

DATE PREPARED:

GRADING SYSTEM:

REQUIREMENTS OF THE COURSE:

PREPARED BY: (By the faculty /team; signature over printed name; date)

RECOMMENDED BY: (By the dean, signature over printed name; date)

APPROVED BY: (By the VP-Academics/Academic Director; signature over printed name; date)