5/31/2018

Scenario: Selena is suspected

to have killed by an
accidental paracetamol
overdose
You need to investigate if:
1. Her cause of death is due to high dose of paracetamol
2. What is the concentration of paracetamol in her blood

By Dr Tan Suk Fei (Sophie)

School of Pharmacy
Management & Science University
1

INTRUMENTATION & MATERIALS

1. Instrument
a) HPLC

2. Materials
a) Stock solution of 500 µg/mL of paracetamol solution
b) Pipette
c) Mobile phase (1:3 Methanol:water)
d) Lab manual (Detection of paracetamol with HPLC)
e) Syringe
f) Syringe filter

Dr. Tan SF 2

1
 5/31/2018

What you need to do?

1. Prepare a standard curve with these following concentration:

 100, 50, 25, 12.5, 6.25, 5 ,1, 0.05 µg/mL of paracetamol standard

2. Run all these standard solution with HPLC

3. Construct a standard curve with HPLC software
4. Run the sample
5. Quantify the sample by calculating it with standard curve that you have
constructed

Dr. Tan SF 3

Introduction

 What is paracetamol and how it works?

 What is pathophysiology of paracetamol (Hepatotoxic)?
 What is HPLC and how can it helps in your investigation?

Dr. Tan SF 4

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Result & Discussion

1. **What is the condition of HPLC in analysing the paracetamol ?

a) What column?
b) Temperature?
c) Flow rate?
d) What mobile phase? [4]
2. Is paracetamol found in her blood sample? [1]
3. If yes, what is the concentration of paracetamol found in her blood sample (Should
the calculation of how you quantify that) [2]
4. Is she died of overdose of paracetamol? Why? [2]
5. Conclusion [1]

Dr. Tan SF 5

Dr. Tan SF 6