ENZYMES/ NAME OF TEST PRINCIPLE REAGENT PRODUCT WAVELENGTH REFERENCE ADDITIONAL INFO

ELECTROLYTES RANGE
Lactate Wroblewski-Cabaud Colorimetric test that utilizes LACTATE+
Dehydrogenase NAD forming PYRUVATE+ NADH
DNPH GOLDEN BROWN 440-525nm
Pyruvate produced reacts with DNPH at PHENYLHYDRAZINE
an ALKALINE pH
Lactate Wroblewski- LaDue Spectrophotometric test based on the -DECREASED IN
Dehydrogenase conversion of 340nm 80-280 U/L at ABSORBANCE is
PYRUVATE TO LACTATE 37o monitored

-NADH acts as a co-

substrate
Lactate WACKER Spectrophotometric test based on the - INCREASED IN
Dehydrogenase conversion of 100-225 U/L at ABSORBANCE is
LACTATE TO PYRUVATE 37o monitored

-NADH is generated during

the reaction
Amylase Caraway This measures the decrease in loss of the blue color -It is also known as the
( Amyloclastic substrate concentration when iodine is mixed iodimetric method
Method) with starch -It uses I2, as indicator

Amylase Saccharogenic Method This measures the products based on

their reducing properties
Amylase Amylometric Method This measures the amount of starch similar to amyloclastic
hydrolyzed within a specified time
Amylase Chromometric This measures the time required for
Methods complete hydrolysis of a substrate
Amylase Chromolytic Methods This measures the amount of soluble
dye released from an insoluble starch-
dye complex
Amylase Teitz method The hydrogen peroxide produced is Uses starch, Forms gluconic acid and Coupled Kinetic Method
coupled with oxidation of chromogen maltose, and hydrogen peroxide
glucose (coupled w/ chromogen)
: -Olive oil
Lipase Cherry-Crandall -lipase hydrolyzes triglycerides in olive -NaOH Lipase is increased in
(Titremetric method) oil -Phenolphthalein 0-1.0 U/mL acute pancreatitis
as the indicator
-fatty acid released is titrated with
NaOH using phenolphthalein as the
indicator
Lipase modified Cherry-Crandall
Henry-Sobel- -pH meter or a
Berkman method thymolphthalein
(Titremetric method) indicator is used.

Lipase Shihabi-Bishop and Olive oil as

Sigma-Teitz substrate
(Turbidimetric)

LAP Goldbarg-Rutenburg The β-naphthylamide reacts with Ethylene diamine Male:

method ethylene diamine dihydrochloride dihyrochloride blue product 80 to 200 U/mL

Female:
75 to 185 U/mL

Female : Used as a marker of liver

GGT Szasz method p-nitroanilide as 0 - 45 U/L damage
. the substrate
Male : Used along with LAP and
0 - 65 U/L 5’N to study hepatobiliary
diseases.
 ENZYMES/ NAME OF TEST PRINCIPLE REAGENT PRODUCT WAVELENGTH REFERENCE ADDITIONAL INFO
ELECTROLYTES RANGE

Increased in post-hepatic
jaundice, intrahepatic
5Nucleotidase 5AMP as cholestasis, and infiltrative
substrate lesions of liver

-It has only slight increase

in hepatocellular jaundice

-Normal in bone disease