HONEY EXTRACT AS GROWTH INHIBITORS AGAINST Escherichia coli,

Staphylococcus aureus, and Aspergillus fumigatus

LIFE SCIENCE-TEAM CATEGORY

An Investigatory Project

Presented to the Board of Judges as Entry to

The 2014-2015 Division Science Fair

BONGABON NATIONAL HIGH SCHOOL

Bongabon, Nueva Ecija

By:

Reala N. Zabat

Mrs. Mary Claire C. Payawal

Project Adviser

August 2014
 ABSTRACT

The present study entitled HONEY EXTRACT AS GROWTH INHIBITORS

AGAINST Escherichia coli, Staphylococcus aureus, and Aspergillus fumigatus
aims to determine whether honey can inhibit bacterial growth.
Different test was conducted to determine the antibacterial and antifungal
properties of honey extract in varying concentrations; T1-100%, T2-50%, and T3-
10%.There were also T4-positive control and T5-negative controls. The test organisms
used were Escherichia coli, Staphylococcus aureus and Aspergillus fumigatus. These
subjects were tested and measured through their zones of inhibition.
In vitro bioassay test results revealed that Treatment 1 (100%) honey extract
inhibit the growth of Escherichia coli at 0 mm zone of inhibition as well as in Treatment 2
(50%) extract, Treatment 3 (10%) extract and Treatment 5 (negative control) while the
Treatment 4 that is the positive control (penicillin) shows 21.95 mm zone of inhibition.
This implies that only the positive control (penicillin) has the antibacterial property
against E.coli.
Results also revealed that Treatment 1 (100%) honey extract significantly inhibit
the growth of Staphylococcus aureus at 16.23 mm followed by Treatment 2 (50%) and
Treatment 3 (10%) that showed no zone of inhibition. Positive control shows 45.43 mm
zone of inhibition. Negative control shows no zone of inhibition. This also shows that the
100% honey extract concentration showed 16.23 mm zone of inhibition and positive
control shows 45.43 mm zone of inhibition against Staphylococcus aureus. This implies
that both treatments extract exhibit antibacterial property against the test organism.
The positive control on the Aspergillus fumigatus showed 24.98 mm zone of
inhibition. This was followed by 50%, 10% and negative treatment of honey extract with
no significant differences. The 100% honey extract showed 44.02 mm. In the case of
Honey extract against Aspergillus fumigatus the 100% honey extract (44.02mm) is more
effective than the positive control (24.98mm) that implies that the honey extract has
anti-fungal property against Aspergillus fumigatus.
It was concluded honey extract is best in treating fungal organisms.

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 ACKNOWLEDGMENT

The writers are grateful to many people who gave the much-needed support and

assistance to bring this task into completion. Without their assistance, this piece of

work could not have been possible.

First of all, to our Almighty God, for his countless blessings, guidance, and love

and for whom we believed greatly helped us in our studies.

Our beloved research adviser and friend, Mrs. Mary Claire C. Payawal, for her

patience, technical and moral support, brilliant suggestions, and for being there with us

throughout the study;

Mrs. Gemerlyn G. Garcia,Ph.D, Veterinary Microbiology College of Veterinary

Medicine , Central Luzon State University, for helping us in the microbial testing of the

product;

To our Science Department Head, Mrs. Rosemarie Z. Burayag, for her support

on all BNHS student’s undertakings;

To our beloved Principal Madam Celia M. Inducil for her suggestions and moral

support for the development of the study.

To all IV-Cattleya students, especially to Asie, Keith, Samantha, Angelica,

Michael and Carlos, for their moral support and cooperation to make this study

complete;

All the teachers, specially to our beloved adviser Ma’am Marivic C. Calara, and

all students of Bongabon National High School for helping us raised funds for our

project; and

Finally, to our parents, sisters, friends, and love ones who stood by through the

radiant moments of joys and in the darkest of our needs to accomplish this research

study.

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 Table of Contents

Abstract ii

Acknowledgment iii

Table of Contents iv

I. Introduction

A. Background of the Study 1-2

B. Statement of the Problem 2-3

C. Hypothesis of the Study 3

D. Significance of the Study 3-4

E. Scope and Delimitation 5

II. Review of Related Literature and Studies 6-13

III. Methodology

A. Materials 14

B. Experimental Design 14

C. Methods 15-17

D. Data Gathering 17

IV. Results and Discussion 18-23

V. Summary, Conclusion and Recommendation 24-25

VI. Bibliography 26-27

VII. Appendices 28-37

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