Abstract
The purpose of this study was to prove and determine
antioxidant activity of leaf extracts from Pateteg (Selliguea
taeniata) and Behel api (Elaeagnus philippinensis) and compile
summary of their content substances and effects. Selliguea
taeniata and Elaeagnus philippinensis are often used in natural
therapy and food industry. Method DPPH (2,2-difenyl-1-
pikrylhydrazyl) was used for determination antioxidant activity
of methanolic leaf extracts. Results of antioxidant activity were
expressed as IC50 values (concentration of extract which causes
50% reduction of radical). The average observed value is 0.884
mg/ml. Deviations can be caused by ongoing synthesis of the
secondary metabolites.
Background of the Study
Elaeagnus philippinensis is a large Philippine shrub often used as an
ornamental in the south because of its lovely silver green leaf. Through
phytochemical test, it was discovered that it contains saponins, steroids,
tannins, and flavonoids. Selliguea taeniata is an indigenous plant of
Kankana-ey, Mt. Province. Through phytochemical test, it was
discovered that Pateteg (Selliguea taeniata) leaf contains carbohydrates,
phytosterols, phenolics, flavonoids and proteins. These plants contain
flavonoid that is a source of antioxidant. This motivated the researchers
to conduct a study to screen and evaluate the antioxidant activity of
these two plants when mixed together.
Statement of the Problem
This study aims to test the antioxidant property both
plants combined.
1) How do the chemical components present in Selliguea taeniata leaf
compare with that of Elaeagnus philippinensis leaf?
2)Is there a significant difference between the different combinations of
Selliguea taeniata (Pateteg) and Elaeagnus philippinensis (Behel api)
leaves extracts and the positive control through antioxidant activity
based on DPPH absorbance readings?
3) Is there a significant difference among the treatments through
antioxidant activity based on DPPH absorbance readings?
Significance of the Study
In our present society, herbal medicines have become
increasingly popular. We know as used that medicinal herbs are the
basis of the pharmaceutical industry, drugs can be derived from plants
and it cannot deny the fact that it will always be useful in terms of
therapeutic values. Many people especially old ones care so much of
their health that they use medicines and vitamins to boost or maintain
their health and to avoid diseases. This study determines the antioxidant
potential of Elaeagnus philippinensis mixed with Selliguea taeniata
leaf extract, so that it would be useful in making new herbal medicines.
THE ANTIOXIDANT POTENTIAL OF Conclusion
1)Both Selliguea taeniata leaf and Elaeagnus philippinensis
SELLIGUEA TAENIATA (PATETEG) leaf contain flavonoids and they also both don’t contain alkaloids and
anthraquinones. While Elaeagnus philippinensis leaf has tannins that
Selliguea taeniata leaf does not have.
AND ELAEAGNUS PHILIPINNENSIS 2)There is a significant difference between the different
combinations of Elaeagnus philippinensis and Elaeagnus
(BEHEL API) LEAF EXTRACTS philippinensis leaves and the positive control, wherein treatment 5 is
higher than treatment 6 and 7 which are positive controls and
treatment 4 and 3 are higher than treatment 6.
Methodology
3)There is a significant difference between the different
treatments, wherein treatment 10, 9, and 8 are significantly different
Gathering of Materials Incubation of the treatments mixed with each other, but treatment 8 and 5 have the same effect; treatment
Preparation of Materials
with DPPH solution at 37° C for 30 5 has higher effect than treatments 11, 7, 6, 3, 2, 1, and has lower
minutes effect than treatments 10, and 9. Treatment 4 has lower effect than
Preparation of Elaeagnus treatments 10, 9, 8, and has higher effect than treatments 2, 1, 11, but
Absorbance reading through
philippinensis and Selliguea taeniata has the same effect as treatment 5; treatment 1 and 2 are not
spectrophotometer at 517 nm
leaf extracts significantly different than the treatment 11, which is the negative
Interpreting results in DPPH assay control.
Preparation of DPPH solution
Recommendation
Statistical Analysis 1)The phytochemical analysis/testing of Selliguea taeniata
Mixing of Elaeagnus philippinensis
and Selliguea taeniata leaf extracts leaf and Elaeagnus philippinensis leaf should be repeated since the
Conclusion
and the controls to the DPPH solution constituents tested on the leaves were not the same.
2)The experimentation between the different treatments of
Selliguea taeniata leaf extract and Elaeagnus philippinensis leaf
Results and Discussion
extract should be repeated to improve the consistency of results.
Selliguea taeniata leaf extract having flavonoids and phenolic proves that it 3)The computation of results should also be repeated to
has antiviral, anti-allergic, antiplatelet, anti-inflammatory, antitumor, and antioxidant correct any mistakes on the DPPH absorbamce reading, ANOVA and
activities. Elaeagnus philippinensis leaf extract has antiviral, anti-allergic, antiplatelet, Duncan reading.
anti-inflammatory, antitumor, and antioxidant activities because of flavonoid. It is
capable of reducing inflammation because of steroids. And because of saponins it Bibliography
contains, it is capable of producing soap. treatment 10, ascorbic acid (100µg/ml), has Alindayo, A. C., & Daoay, S. Z. Antimicrobial and
the highest free radical inhibition of 94.598 % and lowest mean absorbance of 0.064. antioxidant potential of Selliguea taeniata (Pateteg) leaf extract. Baguio
This means that it has the highest antioxidant activity, because the lesser the mean City National High School, Baguio City, 2013
absorbance, the higher the activity in antioxidants. It is then followed by treatment 9, Capdos, Aaron A. Antioxidant property of Elaeagnus
also ascorbic acid, which has 68.206 % free radical inhibition and 0.379 mean philippinensis leaf extract. Cordillera Regional High School, La
absorbance. Then followed by treatment 8, 5, 4, 7, 3, 6, 2, 1, and lastly treatment 11, Trinidad, Benguet, 2013
the negative control, which don’t have a zone of inhibition. The computed f value, Fc =
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50.571, is greater than the tabulated f value, which is 2.2967 at .05 level. This means
that there is a significant difference between the different treatments. The difference
between T5 and T8 at 0.127 is lesser than the computed least significant range for two
samples, R2 at 0.139, it is concluded that there is no significant difference between the
combination of extracts (1000µg/mL) and the positive control (100µg).