MATERIALS AND METHODS

COLLECTION OF MEDICINAL PLANTS :-

Fresh and healthy plants were collected for over three months time (Oct-
Dec 2017) from various locations of the kanyakumari District (Attoor) of these
plants two medicinal plants were selected for the study.

Andrographics paniculata, Nees.

Division - Magnoliophyta

Class - Magnoliopsida

Sub Class - Dilleniidae

Order - Capparales

Family - Moringaceae

Genus - Moringa

Species - oleifera

Moringa oleifera is the most widely cultivated species of a monogeneric

family, the Moringaceae, which is native to the Sub – Himalayan tracts of India,
Pakistan, Bangladesh and Afghanistan. India is the largest producer of Moringa
with an animal production of 1.1 to 1.3 million tones of tender fruits from an area
of 380 Km2.
 Moringa oleifera is a short, slender, deoduous, perennial tree to about 10m
tall rather slender with drooping branches corhy bark, leaves fieathery, pale green,
compound, tripinnate 30, 60cm long with many small leaflets. Flowers fragrant,
white or creamy, stamens yellow pods pendulous, main root thick.

PREPARATION OF EXTRACT

Freshly collected plant leaves were shade dried for ten days and grind. The
grind powder was soaked in 10ml of different solvents such as ethanol, n-butyl
alcohol, iso - propyl alcohol, benzene and acetone. All the solvent extracts were
kept at room temperature for 10 days with periodic shacking to extract the
compounds from the powdered material. The extracts were obtained by passing
through the muslin cloth, the respective solvents were evaporated with the help of
heating mantle. Then it was stored in air tight bottles for further study.

PREVENTION OF NATURAL DISC

Sterile discs were obtained and stored at 4°C. Discs were handled using a
pair of presterilized forceps. The extract was loaded on to the disc carefully using
capillary tube, without spreading out. Thus the disc completely saturated with the
extract was used for testing antibacterial activity.

SYNTHETIC DISC

The synthetic discs used were chloramphenicol, Kanamycin and neomycin.

HUMAN PATHOGENIC USED

A total of five human pathogens were used in the study staphylococcus

aurous, Escherichia coli, proteus vulgaris, pseudomonas aeruginosa and salmonella
typhi. All human pathogens were purchased from the (Scudder laboratory,
Nagercoil)

CULTURE OF TEST HUMAN PATHOGENS (DILUTION)

An inoculums of each pathogen was suspended in 3m1 of nutrient broth. The

bacteria species were cultured in the nutrient broth. All culture were incubated at
37°C for l8hr and then diluted to 1/10 the concentration to yield a culture density
of approximately 108 CFU/ML.