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CONTACT

45-1 Ramsey Road, Shirley, NY 11967, USA


Tel: 1-631-619-7922
Email: contact@cd-genomics.com
Web: https://www.cd-genomics.com

Viral Metagenomic Sequencing Service


By CD Genomics

As an experienced provider of NGS services and a partner of Illumina, CD


Genomics is committed to offering unprecedented amounts of sequence data
permitting rapid and innovative analytical approaches with cost-effective
solutions. We have strong expertise in providing the confident and unbiased
viral metagenomic sequencing service by employing state-of-the-art high
throughput sequencers, sequencing strategies, and bioinformatics pipelines.

The Introduction of Viral Metagenomic Sequencing

Viruses are the most abundant biological entities in the world which
outnumber microbial cells 10:1 in most environments. Viruses constantly
inhabit our body, and asymptomatic hosts are no exceptions. This emerging
vision raises the need for the exploration of the virome. Viral metagenomics
can provide insights into the composition and structure of viral communities.
Profiling the taxonomic composition of viral communities is important not only
for basic research but also for clinical science and practice.

However, viral communities are difficult to be characterized because there is


no a single gene that shared by all viral genomes, which limits the application
of analogous methods used in bacteria for ribosomal DNA profiling. The low-
abundance of free and cellular DNA is another challenge. Metagenomic
shotgun sequencing and RNA-seq are two approaches for the
characterization of viral communities. Direct sequencing can result in a high
background of genetic materials. Therefore, extra procedures are needed to
concentrate and purify viral particles (VPs). Multiple viable enrichment
methods have been suggested, including filtration, ultracentrifugation,
nuclease treatment, multiple displacement amplification (MDA), linker-
amplified shotgun library (LASL), and random PCR approach.

Advantages of Viral Metagenomic Sequencing

• No need for cultivation or antibody laboratory tests


• Comprehensive information on community biodiversity, taxonomy, and
function
CONTACT
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-619-7922
Email: contact@cd-genomics.com
Web: https://www.cd-genomics.com

• Promising applications include virus discovery and viral pathogen


identification associated with horticulture, veterinary medicine, and human
health, especially for the difficult-to-diagnose cases.

Viral Metagenomic Sequencing Workflow

Our highly experienced expert team executes quality management by


following every procedure to ensure comprehensive and accurate results. The
general workflow for viral metagenomic shotgun sequencing is outlined below.
Briefly, the basic steps involved in viral metagenomics include the isolation
and purification of viral particles by size filtration or density-based
centrifugation, sequence-independent amplification of viral nucleic acid,
shotgun sequencing or RNA-seq and matched analyses on diversity,
taxonomy, and function through a battery of reliable bioinformatics tools.

Service Specification

Apart from the qualified viral metagenomic sequencing, we provide


assistance, including the experimental design, the determination of the
CONTACT
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-619-7922
Email: contact@cd-genomics.com
Web: https://www.cd-genomics.com

appropriate sequencing platform, software tools, and analysis methodologies,


to accommodate your project. If you have additional requirements or
questions, please do not hesitate to contact us, our experienced specialists
would like to help you to solve your questions.

References:

1. Li Y, et al. VIP: an integrated pipeline for metagenomics of virus identification


and discovery. Scientific reports, 2016, 6: 3774
2. Rampelli, S. et al. ViromeScan: a new tool for metagenomic viral community
profiling. BMC Genomics. 2016, (17):165.
3. Lewandowska D W, Zagordi O, Geissberger F D, et al. Optimization and
validation of sample preparation for metagenomic sequencing of viruses in
clinical samples. Microbiome, 2017, 5(1): 94.