C.

Primer design and compositional analysis

1. Search for the longest transcript of the human BRCA2 gene from Ensembl. Retrieve the sequences of
the exons. Using Primer3Plus, design primers that would amplify exon 16. Provide a screenshot of your
top result.

EXON 14 Sequence:

CTGTATACGTATGGCGTTTCTAAACATTGCATAAAAATTAACAGCAAAAATGCAGAGTCT

TTTCAGTTTCACACTGAAGATTATTTTGGTAAGGAAAGTTTATGGACTGGAAAAGGAATA

CAGTTGGCTGATGGTGGATGGCTCATACCCTCCAATGATGGAAAGGCTGGAAAAGAAGAA

TTTTATAG

2. Show that the PCR product using your primers is unique. Provide a screenshot of your analysis.
3. Using the Genomatix DNA Sequence Toolbox, determine the GC content of the PCR product in item
C2.

GC-content 38.41%