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MEIJO UNIVERSITY

Faculty of Agriculture
2012
Postgraduate Courses in Agriculture and Facuty of Agriculture

POSTGRADUATE COURSEs IN AGRICULTURE FACULTY OF AGRICULTURE

Special Course in Agrobiological Resources Department of Agrobiological Resources

Plant Production Science Laboratory of Crop Science 1

Laboratory of Horticultural Science 2

Genetics Laboratory of Genetics & Breeding Science 3

Laboratory of Plant Molecular Genetics 4

Plant Protection & Biodiversity Laboratory of Plant Pathology 5

Laboratory of Entomology 6

Agricultural & Resource Economics Laboratory of Agricultural & Resource Economics 7

Special Course in Applied Biological Chemistry Department of Applied Biological Chemistry

Life Science Laboratory of Microbiology 8

Laboratory of Biological Chemistry 9

Food Science Laboratory of Nutrition & Food Science 10

Laboratory of Functional Food Science & Technology 11

Bio-organic Chemistry Laboratory of Biophysical Chemistry 12

Laboratory of Natural Organic Chemistry 13

Laboratory of Pesticide Chemistry 14

Special Course in Environmental Bioscience Department of Environmental Bioscience

Environmental Bioscience Laboratory of Plant Conservation Science 15

Laboratory of Environmental Zoology 16

Dynamic Soil Science Laboratory of Environmental Analytical Chemistry 17

Laboratory of Environmental Soil Science 18

Bioremediation & Environmental Response Laboratory of Plant Physiology & Function 19

Laboratory of Environmental Microbiology 20

Landscape Design Laboratory of Landscape Design 21

EXPERIMENTAL FARM 22
Laboratory of Plant & Animal Science (Field Science)

[Curriculum]

Curriculum of Faculty of Agriculture, Curriculum for Bachelor's Degree 23


Meijo University in 2012 Common Liberal Curriculum 24

Curriculum for Advanced Doctral Course of Agriculture


POSTGRADUATE PROGRAM OF AGRICULTURE 25
Curriculum for Master's Course of Agriculture
More Information

How to apply for admission to the faculty of Agriculture.


1. Confirm your requirements for admission to Meijo university: At the middle of
November. You should be a good command of Japanese and stay in Japan.
2. Application procedure: At the beginning of January. Examination fee: 35,000yen.
3. Entrance examination: The first of February, at Meijo university, Tempaku, Nagoya.
4. School registration: By the middle of March.
5. Entrance ceremony: At the beginning of April. (1st April)

How to take the postgraduate course in Agriculture.


[Entrance at April]
1. Confirm your requirements for admission to Meijo university:
1) At the middle of May. 2) At the late of September.
2. Application procedure:
1) At the middle of June. 2) At the late of October.
3. Entrance examination: Examination fee: 35,000yen.
1) At the beginning of July. 2) At the beginning of November.
4. School registration: By the middle of March.
5. Entrance ceremony: At the beginning of April. (1st April)

[Entrance at September]
1. To confirm your requirements for admission to Meijo university:
At the middle of May.
2. Application procedure: At the middle of June.
3. Entrance examination: At the beginning of July.
4. School registration: Same above.
5. Entrance ceremony: At 20th of September, the current year.

E-mail for admission (Admission Office): nyugaku@ccmails.meijo-u.ac.jp


E-mail for another information: kouhou@ccmails.meijo-u.ac.jp

International Exchange Committee, Faculty of Agriculture.


(Edited by Public Service Committee, Faculty of Agriculture.)
Department of Agrobiological Resources

Laboratory of Crop Science


STAFF Professor Hiroyasu MICHIYAMA Associate Professor Tatsuya HIRANO
Food Crops Science Metabolic Physiology
Tropical Agriculture Crop Production Science
TEACHING Plant Resources Advanced Crop Physiology (MC) Professor Associate Professor
Advanced Crop Production Science (MC)
Hiroyasu MICHIYAMA Tatsuya HIRANO

Research
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Recent publications:
・Hirano et al. (2007) Virus-induced gene silencing of 14-3-3 genes abrogates dark repression of nitrate reductase activity in Nicotiana benthamiana. Mol. Genet. Genomics 278: 125-133
・Kumazaki et al. (2008) Effects of day length and air temperature on stem growth and flowering in sesame. Plant Prod. Sci. 11: 178-183
・Hirano et al. (2008) Inhibition of ammonium assimilation restores elongation of seminal rice roots repressed by high levels of exogenous ammonium. Physiol. Plant 134: 183-190
・Kumazaki et al. (2009) Effects of day length and air and soil temperatures on sesamin and sesamolin contents of sesame seed. Plant Prod. Sci. 12: 481-491 1
・Hirano et al. (2011) Identification of two plastid-targeted beta-amylases in rice. Plant Prod. Sci. 14: 318-324 Back
Department of Agrobiological Resources
Laboratory of Horticultural Science
Professor Professor Associate Professor
STAFF
Naosuke NII Shigetoshi SUZUKI Masato TSURO
Horticultural Science Vegetable Crop Science Floricultural Science
Pomology Plant Physiology Cell Technology
Advanced Horticultural Environmental Control in Advanced Horticultural
TEACHING Production Science (MC) Crop Growth and Development Production Science (MC) Professor Professor Associate Professor
Advanced Horticultural Naosuke NII Shigetoshi SUZUKI Masato TSURO
Physiology (MC)

Research

Fruit Study of the relationship between structure and function during


development of fruit, leaf and root

Analysis of fruit growth Structural and functional analysis


and development in root development

Structural and functional Analysis of functional Control 5 days after


analysis in leaf development ingredient in fruit trees Nacl (500mM) treatment

Vegetable Ecophysiological study on


photosynthesis
and water relations

Photosynthesis Interaction between transpiration Water


and environmental factors relations

Salt tolerance

Hydroponics

Flower
Elucidation and utilization of
floricultural characters

Production of useful
Mass propagation secondary metabolites
Tissue culture Development of
Development of a new odor plant
Meristem culture
a flower with a new character Development of a new colored flower
Cell fusion
Genetic transformation
DNA marker
Genetic analysis

Volatile Components
in lavender flower
Recent publications:
・L. Ye, Y. Song, K. Yamada, Y. Nakao and N. Nii (2012) Anatomical and histological changes in developing silverberry (Elaeagnus multiflora var. gigantea L.) fruit. J. Hortic. Sci. Biotech. 87: 64-70.
・Y. Song, L. Ye and N. Nii (2011) Effects of soil water availability on development of suberin lamellae in the endodermis and exodermis and on cortical cell wall thickening in red bayberry (Myrica rubra Sieb. et Zucc.) tree
roots. Sci. Hortic. 129, 554-560.
・Y. Song, L. Ye, T. Astha and N. Nii (2011) Anatomical development of cell structure including Casparian strip during root growth in grapevines. J. Japan. Soc. Hort. Sci. 80, 164-168.
・T. Kaneko and S. Suzuki (2006) Effects of high temperature and growth retardant on dry matter accumulation, hypocotyl thickening, photosynthesis and sugar content in radish (Raphanus sativus L.) plants. J. Japan. Soc.
Hort. Sci. 75, 231-235.
・M.Tsuro and H.Ikedo (2011) Changes in morphological phenotypes and essential oil components in lavandin (Lavandula × intermedia Emeric ex Loisel.) transformed with wild-type Agrobacterium rhizogenes. Sci. Hortic.
130, 647-652.
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Department of Agrobiological Resources
Laboratory of Genetics and
Breeding Science
STAFF Professor Rie TERADA Lecturer Shigeo TANAKA
Genetics Statistics of Agricultural Science I
Professor Lecturer
TEACHING Breeding Science Statistics
Advanced Genetics and Breeding Science (MC) Information Science Rie TERADA Shigeo TANAKA

Research

1. Gene modification by the gene targeting in rice.


The technology of gene targeting (GT) mediated by homologous recombination (HR) is a powerful tool for
genetic engineering and the study of gene function, because GT has a possibility of artificial gene design
through precise nucleotide manipulation. We firstly succeeded GT of Waxy based on a strong positive-
negative (PN) selection in rice (Oryza sativa L.), an important staple food. Fifteen individual rice genes were
subsequently targeted by our GT procedure. Our research purpose is improvements of the GT technol-
ogy and the progression of molecular breeding in rice.

Figure; GT of Waxy resulted in mutant waxy . The waxy phenotype was detected in rice seed and pollen.

2. Improvement of Potato production by True Potato seed.


On potatoes, we are developing TPS (True potato seed) system. This system uses biological seed (TPS)
instead of seed tuber as planting material. TPS is not only to facilitate storage and transport but also to
prevent from infecting many disease intermediate seed tuber.

Recent publications:
・Moritoh, S., Eun, C-H, Ono, A., Asao, H., Okano, Y., Yamaguchi, K., Shimatani, Z., Koizumi, A. and Terada R. (2012) Targeted disruption of an orthologue of DOMAINS REARRANGED METHYLASE2, OsDRM2, impairs
the growth of rice plants by abnormal DNA methylation. The Plant Journal in press
・Ono, A., Yamaguchi, K., Fukada-Tanaka, S., Terada, R., Mitsui, T., Iida, S. (2012) A null mutation of ROS1a for DNA demethylation in rice is not transmittable to progeny. The Plant Journal in press
・Terada, R., Nagahara, M., Furukawa, K., Shimamoto, M., Yamaguchi,K. I., Iida, S.: Cre-loxP mediated marker elimination and gene reactivation at the waxy locus created in rice genome based on strong positive-negative
selection. Plant Biothechnology 27: 29-37 (2010)

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Department of Agrobiological Resources

Laboratory of Plant Molecular Genetics


STAFF Professor Atsushi MORIKAMI
Biological Chemistry
Cell Biology Professor
TEACHING Molecular Biology
Atsushi MORIKAMI
Advanced Molecular Genetics (MC)

Research

Exploration of genes involved


in plant production
Plants contain tens of thousands of
genetic blueprints called “genes” Research projects
involved in the biological processes
that contribute to the growth of plant 1 Regulation of plant gene
cells. Coordinated application of
blueprints allows plants to grow,
expression by sugar
mature, and produce seeds at the end The main aim of agricultural production is to develop
of their life cycle. Fully developed plant foods containing starch, lipids, and proteins. Thus,
organs, such as tuberous roots, stems, clarification of the mechanisms underlying the
leaves, and seeds, are used as food accumulation of these
sources. Individual plants have their Water
products in seeds or
unique set of blueprints, which partially
differ among plants. Whether a
tuberous organs is very
blueprint is associated with good farm important. To address
6%Sucrose
productivity depends on the information this problem, we
contained in the blueprint of each plant. focused on the analysis
of plant genes whose
The recent progress in molecular expression was regu- Emission of light by leaves of a
biology and genetics has led to the lated by different con- transgenic plant under conditions
introduction of techniques to decode centrations of sugar. of high sugar concentrations.
the information contained in a gene.
The techniques yield information about
the product of a genetic blueprint, the
part of the plant in which the product
2 Transcription factors involved
performs its functions, the chief in the control shoot branching
function of the product, and the capac-
Each plant has a unique shape by
ity of the product to carry out its
functions. Depending on the informa- which the species of the plant can be
tion, a plant with superior traits and an classified; the shape of a plant is
ideal combination of genes could be governed by a strict control of gene
selected to increase the agricultural expression that determines the
output. timing and direction of cell divisions.
The shape of a plant affects crop
In the future, the population and productivity and efficiency of works
demand for energy are thought to on the farm. Therefore, in our labora-
increase worldwide. We think that Specific expres-
tory, shoot branching was studied sion of the green
studies on plant production are needed
to solve these problems. Therefore, we using molecular methods. In addition, fluorescent protein
investigated the functions of individual we analyzed other genes involved in in the central cells
genes in plants in an attempt to search plant development, such as the size of a root meristem.
for useful genes. of seeds and root structure.

Recent publications:
・Morikami, A et al (2005) Two cis-acting regulatory elements are involved in the sucrose-inducible expression of the sporamin gene promoter from sweet potato in transgenic tobacco. Mol Gen Genomics 272: 690–699
・Inagaki S et al (2006) Arabidopsis TEBICHI with Helicase and DNA Polymerase Domains is Required for Regulated Cell Division and Differentiation in Meristem. Plant Cell 18, 879-892
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Department
Department of Agrobiological
of Agrobioresources Resources

LaboratoryofofPlant
Laboratory PlantPathology
Pathology
STAFF Kimiharu INAGAKI, Professor Masao ARAKAWA, Associate Professor
STAFF
Education Professor
Plant PathologyKimiharu INAGAKI MicrobiologyAssociate Professor Masao ARAKAWA
Plant PathologyMicrobiology
Agroenvironmental Microbiology
Plant Disease Control
TEACHING Advanced Plant Pathology
Agroenvironmental MicrobiologyAdvanced Physiological
Plant DiseasePlant Pathology
Control Dr. KimiharuProfessor
Inagaki Dr. MasaoProfessor
Associate Arakawa
Advanced Plant Pathology (MC) Advanced Physiological Plant Pathology (MC)
KimiharuProfessor
INAGAKI Associate Professor
Masao ARAKAWA

Research
Research
Significant research topics include
- Disease modelings and ecologies of pathogenic fungi
- Biological control of fungal diseases
- Molecular diagnosis of pathogenic fungi
- Population genetics and evolutionary biology
- Adaptability of plant pathogen to agroenvironmental changes

Field Works

Microscopy Macroscopic Observation


Control the Plant Diseases

Using Approaches Ranging

from the Field Experiments

to the Molecular Techniques!

Mycelial Compatibility Fluorescent Probes

Tc03
Continental Japan
Okinawa Japan
Northern China
Eastern China
Central China
Southern China
Western China
DNA Sequencing
India

Populations in Asia 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

SSR Genotyping for Global Population


3D Modeling of the Enzyme
Recent publications:
・Inagaki, K., Guo, Q., Mathur, A. C. and Arakawa, M.(2011)Rice sclerotial disease fungi, Rhizoctonia spp., in relation to their distribution and movement in/between paddy fields. BIT’s Annual World Congress of Microbes-2011, Beijing, China.
・Arakawa, M., Ohkawa, Y. Urushizaki,S. Kato, M. and Inagaki, K. (2008) Designing of PCR primers to discriminate eight sclerotial disease pathogens of rice plant. 9th International Congress of Plant Pathology, Torino, Italy.
Publications
・Guo, Q., Kamio,/ - Inagaki, K.,B.,
A., Sharma, Guo, Q., Mathur,
Sagara, A. C.
Y., Arakawa, M.,and
andArakawa, M.(2006)
Inagaki, K., (2011) Rice sclerotial
Survival disease
and subsequent dispersal Rhizoctonia
fungi, of spp.,
rice sclerotial in relation
disease to their distribution
fungi, Rhizoctonia oryzae andand movement
Rhizoctonia in/between paddy
oryzae-sativae, fields.
in paddy fields.BIT's
Plant Annual World
Disease 90, 615-622.
Congress
・Inagaki,ofK.,Microbes-2011, Beijing,
Guo, Q., and Arakawa, M.,China.
(2004) Overwintering of rice sclerotial disease fungi, Rhizoctonia and Sclerotium spp. in paddy fields in japan. Plant Pathology Journal 3, 65-71.
- Arakawa, M., Ohkawa, Y. Urushizaki, S. Kato, M. and Inagaki, K. (2008) Designing of PCR primers to discriminate eight sclerotial disease pathogens of rice plant. 9th International Congress of Plant Pathology, Torino, Italy.
・Kanematsu,
- Guo, Q., Kamio,S., A.,
Arakawa, M.,B.,
Sharma, Oikawa,Y., Onoue,
Sagara, Y., M., Osaki,
Arakawa, M., H.,
andNakamura, H., (2006)
Inagaki, K., Ikeda, K., Kuga-Uetake,
Survival Y., Nitta, dispersal
and subsequent H., Sasaki,ofA.,rice
Suzaki, K., Yoshida,
sclerotial diseaseK.fungi,
and Matsumoto,
RhizoctoniaN.oryzae
(2004)and
. A Reovirus Causes
Rhizoctonia Hypovirulenceinofpaddy
oryzae-sativae, Rosellinia necatrix.
fields.
Phytopathology
Plant 94: 561-568.
Disease 90, 615-622. 5
- Inagaki, K., Guo, Q., and Arakawa, M., (2004) Overwintering of rice sclerotial disease fungi, Rhizoctonia and Sclerotium spp. in paddy fields in Japan. Plant Pathology Journal 3, 65-71.
- Kanematsu, S., Arakawa, M., Oikawa, Y., Onoue, M., Osaki, H., Nakamura, H., Ikeda, K., Kuga-Uetake, Y., Nitta, H., Sasaki, A., Suzaki, K., Yoshida, K. and Matsumoto, N.(2004). A Reovirus Causes Hypovirulence Back
Department of Agrobiological Resources

Laboratory of Entomology
STAFF Professor Kenzou YAMAGISHI
Fundamental Entomology
Applied Entomology Professor
TEACHING
Zoological Systematic and Morphology
Advanced Entomology and Biodiversity (MC) Kenzou YAMAGISHI

Research

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Recent publications:
・Yamagishi, K. (2004) Generic Composition of Scelionidae (Hymenoptera) in Agricultural Land. Jpn. J. Entomo., (N. S.), 7: 39-54. (in Japanese). Jpn. J. Entomol. (N.S.), 7: 39-54.
・Fursov, V., Shirota, Y., Nomiya, T., & Yamagishi, K. (2002) New fossil Mymarommatid species, Palaeomymar japonicum sp. nov. (Hymenoptera:Mymarommatidae), discoverd in cretaceous amber from Japan. Entomol.
Sci., 5: 51-54.
・Yokoyama, E., Yamagishi, K., & Hara, A. (2003) Structures of the mating-type loci of Cordyceps takaomontana. Appl. Environ. Microbiol., 69: 5019-5022. 6
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Department of Agrobiological Resources
Laboratory of Agricultural and
Resource Economics
STAFF Professor Hideji ISOMAE Associate Professor Shintaro HIRAKO
Agricultural and Resource Economics Food System Economics
Management of Agrobioproduction Statistics of Agricultural Science II Professor Associate Professor
TEACHING Agricultural Policy Advanced Theory of Agricultural and
Hideji ISOMAE Shintaro HIRAKO
Advanced Theory of Farm Management (MC) Resource Economics(MC)

Research

National Land Conservation and Succession of Rural Culture:


Approach from Social Science

1. What is our mission?


Our expertise in food and agricultural economics, management
science, environmental and rural resource economics, and
international economics work in concert to fulfill the mission to
inform and foster the public stewardship and private management of
businesses, organizations, livelihoods, and natural resources.

2. Outlook of our research field and recent issues

Political welfare and agricultural market


Decision making in farming
Management of natural resource
Development of sustainable agriculture
International trading of agricultural products
Agricultural marketing and consumer’s behavior
Industrial structure of rural economy

3. Our recent subjects

a) Economic effect of land improvement


b) Compensation for unexhausted value of improvement
c) Measurement of rice yield fluctuation and uncertainty
d) Stochastic dominance model and econometrical approach
e) Firm organization of food and agricultural sector

Recent publications:
・Isomae, H. (2006) Lease-Period Desired by Agricultural Capitalist. Journal of Rural Economics (Special Issue) 2006: 95-102.
・Isomae, H. (2002) Reconstruction of the Image of Capitalist and Land Improvement. Journal of Rural Economics (Special Issue) 2002: 86-90.
・Hirako, S. (2006) Factors in Extension of Rice Whole Crop Silage Based on Farmers' Evaluation: A Case Study of Dairy Farms in Misato, Saitama. Journal of Rural Economics (Special Issue) 2005: 160-165.
・Hirako, S. et al. (2006) Potential Demand for Rice Whole Crop Silage and Examination of Extension: An Application Choice Based Conjoint Model. Agricultural Information Research 15(2): 165-172. 7
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Department of Applied Biological Chemistry

Laboratory of Microbiology
STAFF Professor Masashi KATO Assistant Professor Motoyuki SHIMIZU
Microbiology I & II Microbial Technology I
TEACHING Microbial Technology II Molecular Biology Professor Assistant Professor
Advanced Microbiology (MC) Biotechnology
Masashi KATO Motoyuki SHIMIZU

Research
We study microbes to boost their potential contributing to
biotechnology.

1. Screening of novel biologically active substances from actinomycetes.

Fig. 1 Inhibitory effect of the Fig. 2 Promotion of the Fig. 3 Inhibition assay of fungal growth
substances produced by strain degradation of filter paper shows antifungal agent production by
No. 3-1 on the !-amylase by cellulases. Streptomyces hetsukaensis.
activity.

2. Studies to elucidate the 3. Application of useful genes for


 regulation of iron  biomass utilization.
 homeostasis by the Hap
 complex in fungi.

Fig. 5 Alignment of partial


Fig. 4 SEM microscopy of 5’-region sequences among
conidia of Aspergillus useful genes in fungi.
nidulans

4. Mutualisms between beetles and fungi.

cultivation
Fig. 6 Ambrosia beetles live in nutritional
symbiosis with ambrosia fungi.

feed

Recent publications:
・Murakoshi Y. et al. (2012) Comparison and characterization of α-amylase inducers in Aspergillus nidulans based on nuclear localization of AmyR. Appl. Microbiol. Biotechnol. in press.
・Shimizu M.et al. (2010) Mechanism of de novo branched-chain amino acid synthesis as alternative electron sink in hypoxic Aspergillus nidulans cells. Appl. Environ. Microbiol. 76, 1507-1515.
・Shimizu M.et al. (2009) Proteomic analysis of Aspergillus nidulans cultured under hypoxic conditions. Proteomics 9, 7-19
・Hortschansky P. et al. (2007) Interaction of HapX with the CCAAT-binding complex-a novel mechanism of gene regulation by iron. EMBO J. 26, 3157-3168.
・Machida M. et al. (2005) Genome sequencing and analysis of Aspergillus oryzae. Nature, 438, 1157-1161. 8
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Department of Applied Biological Chemistry
Laboratory of
Biological Chemistry
STAFF Professor Minoru UJITA Assistant Professor Hiroki OKUMURA
Biological Chemistry I & II Metabolic Biological Chemistry II
Professor Assistant Professor
TEACHING Metabolic Biological Chemistry I Cell Technology
Advanced Biological Chemistry (MC) Protein Engineering Minoru UJITA Hiroki OKUMURA

Research

Biological Chemistry
Proteins, Nucleic Acids, Enzymes and Carbohydrates

Food Immunology Biotechnology


Functional Foods Biomass

Biomass
Receptors Cytokines

Glucans Chitinases
Microorganisms
Reactive Oxygen
Immunocytes Species Chicken
Starch
Immunostimulating Synthases
Activity Functional Feed
Antitumor Effects Functional
Hen Egg Functional Hen Egg Functional Starch
Nutritional Supplements

Reproductive Biochemistry Genetic Engineering


Sperm-egg Interactions Cosmetics
Acrosome Egg-coat
Reaction Degradation
Cosmetics
Tyrosine

Species-specific Block to
Binding Polyspermy
Tyrosinase
Functional and Structural Analyses
of Egg-coat and Sperm Proteins

Reproductive Medicine
Reproductive Control
Melanin Melanin

Recent publications:
・Okumura, H. et al. (2007) Heterocomplex formation and cell-surface accumulation of hen's serum zona pellucida B1 (ZPB1) with ZPC expressed by a mammalian cell line (COS-7): a possible initiating step of
egg-envelope matrix construction. Biol. Reprod. 76, 9-18
・Okumura, H. et al. (2007) Association of chicken zona pellucida glycoprotein (ZP) B1 with ZPC induces formation of ZPB1-ZPC fibrous aggregates containing disulfide-bridged ZPB1 dimer. Biochem. Biophys. Res. Commun. 364, 682-688
・Ujita, M. et al. (2009) Carbohydrate binding specificity of recombinant human macrophage β-glucan receptor dectin-1. Biosci. Biotechnol. Biochem. 73, 237-240
・Han, L.*, Monne, M.*, Okumura, H.* et al. (2010) Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3. Cell 143, 404-415 (*equally contributed)
・Ujita, M. et al. (2011) Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures. J. Biosci. Bioeng. 111, 22-25
・Ujita, M. et al. (2011) Binding specificity of the recombinant cytoplasmic domain of Cordyceps militaris β-1,3-glucan synthase catalytic subunit. Biosci. Biotechnol. Biochem. 75, 171-174 9
・Okumura, H. et al. (2012) Diverse lectin-binding specificity of four ZP3 glycoprotein isoforms with a discrete isoelectric point in chicken egg coat. Biochem. Biophys. Res. Commun. 424, 586-592 Back
Department of Applied Biological Chemistry
Laboratory of
Nutrition and Food Science
STAFF Professor Akihiro OHARA Associate Professor Ken-ichiro MINATO
Nutritive Science I Nutritive Science II
Food Hygiene Food Chemistry Professor Associate Professor
TEACHING
Food Palatability I Food Resources I
Akihiro OHARA Ken-ichiro MINATO
Food Habits Advanced Food Hygiene (MC)

Research

Major Research Objective :


To understand the function of food ingredients which
contribute to our health care
Food Science
1) Screening of food factors such as anti-
carcinogenic component in vegetables, fruits
and mushroom
2) Mechanism of activity of immunomodulating
factors in vegetables, fruits and mushroom
3) Evaluation of antioxidative activity of foods
4) Development of dental-care compound
Screening of antimutagenicities of
medicinal and/or edible plants in
Hokkaido

Nutritional Science
1) Where a food factor works most
effectively?
2) How food factors prevent from developing
various diseases?

Food factor showed the cytotoxicity to


cancer cell (HeLa cell)

Nutrition & Food Science


1) Assessment of oxidative stress marker
2) When and to whom food factors are
prescribed?

Since 1950
Recent publications:
・K. Minato (2010) Mushrooms: Immunomodulative activity and role in health promotion., in Dietary Components and Immune Function – Prevention and Treatment of Disease and Cancer,” ed. by R. R. Watson, S. Zibadi
and V. R. Preedy, Springer, 529-539
・Akihiro Ohara, Fumie Saito and Tsugio Matsuhisa:(2008) Screening of antibacterial activities of edible plants against Streptococcus mutans,Food Sci. & Technol. Res.,
14, 190∼193
・Minato K.:(2008) A polysaccharide fraction from an edible mushroom, Pleurotus cornucopiae var. citrinopileatus stimulates an immunomodulating action of murine macrophage, RAW264, on TNF-α and NO productions
in vitro, Int. J. Medicin. Mushrooms 10, 235-244 10
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Department of Applied Biological Chemistry
Laboratory of Functional Food
Science and Technology
STAFF Professor Seiichi HAGA Associate Professor Toshiya HAYASHI
Food Science & Technology I & II Food Functionality
Professor Associate Professor
Food Preservation Food Palatability II
TEACHING Seiichi HAGA Toshiya HAYASHI
Advanced Food Science and Technology (MC) Food Resources II
Advanced Functional Food Science (MC)

Research

Research on food processing technology and


functional improvement of animal products
Source of Animal Protein Traditional and Advanced
(Meat, Milk and Egg)→ Food-Processing Technology

Fermentation Retort Processing Food Freezing


Our research subjects:
①Practical application of mass spectral identification of food-related bacteria
②Improvement of palatability and food functionality of animal products by
food processing technique
③Establishment of objective criteria for the texture evaluation based on food structure

① ②


Lab life

Recent publications:
・Hayashi, T., Washio, S., Arakawa, M., Taguchi, M., Toyoda, N. and Haga, S. (2011) Evaluation of the functional properties in pork meat fermented by psychrotrophic bacteria, Int. Cong. Meat Sci. Technol., 57, P395: 1-4.
・Aota, K., Ichinoseki, S., Numata, M., Kosai, K., Miyaguchi, Y., Hayashi, T. and Haga, S. (2011) A new processing method to reduce sodium in sausages without potassium chloride and phosphates, Int. Cong. Meat Sci. Technol., 57, P375: 1-4.
・Hayashi, T., Toyoda, N., Arakawa, M. and Haga, S. (2010) Retort processing may induce protein degradation and improve food functionalities of meat products. Int. Cong. Meat Sci. Technol., 56, E60: 1-4.
・Haga, S., Hayashi, T., Ohba, M. and Sakata, R. (2009)Muscle protein degradation in pork meat fermented by psychrotrophic lactic acid bacteria, J. Res. Inst. Meijo Univ., 8: 91-96 (in Japanese).
・Hayashi, T., Hattori, A., Kato, K., Fujino, T. and Haga, S.
(2009)Comparison of whey protein and water-soluble vitamin contents in the direct and indirect UHT-processed milk –Effect of pre-heating on the residual whey protein and
water-soluble vitamin contents in UHT milk–, Anim. Sci. J., 80: 41-45 (in Japanese).
・Haga, S., Hayashi, T., Ogawa, Y., Taguchi, M., Arakawa, M., Sakata, R. and Nishikawa, J. (2008)Practical training education for acquiring the safety and security of the food, Bull. Res. Inst. Meijo Univ., 13: 5-8 (in Japanese).
・Hayashi, T., Kato, K. and Haga, S.(2008) Angiotensin I-converting enzyme inhibitory peptide derived from porcine skeletal muscle myosin fermented by Lactobacillus lactis subsp.lactis IFO-12007, J. Res. Inst. Meijo Univ., 7: 71-80.
・Teramoto, K., Sato, H., Sun, L., Torimura, M., Tao, H., Waguri, S., Hayashi, T. and Haga, S.(2007) Rapid identification and classification of psychrotrophic lactic acid bacteria by matrix-assisted laser desorption/ionization mass
spectrometry, Bunseki Kagaku, 56: 1063-1070 (in Japanese).
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Department of Applied Biological Chemistry
Laboratory of
Biophysical Chemistry
STAFF Professor Masaharu OHBA Associate Professor Masahiro MAEBAYASHI
Physical Chemistry I & II Analytical Chemistry
Professor Associate Professor
TEACHING Bio-physical Chemistry Instrumental Analysis
Advanced Biophysical Chemistry (MC) Advanced Biophysical Chemistry (MC) Masaharu OHBA Masahiro MAEBAYASHI

Research
Biophysical Chemistry
To study the life phenomena and biologically relevant
materials by using physico chemical methods.

Behavior of the biologically-relevant molecules in aqueous solutions


Life was born in the magical liquid “water”. We can never discover the essence of life without understanding
relationship between the water molecules and the organic compounds. We are now investigating spontaneous
structure changes of organic compounds such as sugars and proteins in aqueous solutions through the
measurements of thermodynamic quantities of the compounds dissolved in the aqueous solutions.
CH2OH Anomeric CH2OH
O
H H Isomerization H
O
OH
Heating
H H

HO OH H OH HO OH H H
Unfolding
H OH H OH Cooling
α-D-Glucose β-D-Glucose
Refolding
K+, Rb+ and Ce+ affect the anomeric proportion Native structure Denatured structure
Structure change of glucose Unfolding and refolding of a protein molecule
in alkali chloride aqueous solutions.

Elucidation of the properties of supercritical fluids and reaction in these fluids


The properties of the supercritical fluids have been applied in the fields of agriculture such as food
chemistry and pesticide chemistry. We are investigating theoretically and experimentally the properties
and the reaction in the supercritical fluids.
Reactor for high pressure and temperature
Solid

Liquid
SCF Supercritical fluids(SCF) Thermostat
Pressure

・Neither liquid nor gas.


・Higher pressure and temperature Temp. &
Critical point pressure
than the critical point. gauge
Gas ・Density, viscosity, diffusivity etc.
can be varied continuously. Back pressure
regulator
Temperature
Phase diagram

Thermal and viscoelastic evaluations for food in its manufacturing and degeneration processes
Amylopectin Retrogradation
Solution
Jelly Amylose
(sol) Exothermic
(gel)
Exothermic,
Gelatinization

Random coil Helix Starch retrogradation in cooked rice and bread


Thermal and viscoelastic properties of food change in the processing and degeneration processes such as the
gelatinization of solutions and the retrogradation of starch . We are investigating the relationship of those
properties to the conditions and quality of the food through the real-time measurements.

Recent publications:
・Ohba, M., M. Maebayashi, S. Harada and H. Sahara, (2008) The Retrograding Process of Cooked Rice and Bread - The Analysis of the Thermal Output by the Monte-Carlo Simulation. J. Res. Institute of Meijo Univ., 7: 61-69. (in
Japanese)
・Maebayashi, M., S. Koda and M. Ohba (2008) Acoustic Analysis of Boundary Layers within Polymeric Composite Materials Containing Inorganic Small Particles. Netsu Sokutei, 35(2): 82-92. (in Japanese)
・Y. Asakura, M. Maebayashi and S. Koda, (2007) Characterization of Sonochemical Reactors by Chemical Dosimetry, Electron. Comm. Jpn. III, 90, 1-8.
・T. Matsuoka, Y. Nakamura, M. Maebayashi and S. Koda, (2006) Surface Wave Velocity in Methylcellulose Hydrogel in the Drying Process, J. Chem. Eng. Jpn., 39, 864-868.
・M. Maebayashi, M. Endo, T. Matsuoka, S. Koda and Y. Isono, (2006) " Acoustic Analysis of Bound Rubber Formed in Silica/SBR Compounds ", Ultrasonics, 44, E1101-E-1104.
・Ohba M. (2004) The Thermodynamic and Spectroscopic Study on the Solvation of the Glucose and Its Linear Origomers. Bull. Res. Institute of Meijo Univ., 9: 93-98. (in Japanese)
・T. Takigawa, M. Ohba, H. Ogawa, S. Murakami, (2003) ''Thermodynamic properties of binary mixtures of hexane isomer and cyclohexane at 298.15K'', Fluid Phase Equilibria, 204, 119-130. 12
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Department of Applied Biological Chemistry
Laboratory of
Natural Organic Chemistry
STAFF Professor Masato MATSUGI Associate Professor Hiromi HAMAMOTO
Organic Chemistry I & II Bio-organic Chemistry I & II Professor Associate Professor
TEACHING Organic Natural Product Chemistry Advanced Bioorganic Chemistry (MC)
Advanced Synthetic Organic Chemistry (MC)
Masato MATSUGI Hiromi HAMAMOTO

Research

Development of New Applicable Methods to Synthesize Bioactive Compounds


The major research efforts of our group are directed toward the development of new applicable methods to synthesize
various bioactive compounds.

Organic syntheses utilizing a light or Development of novel reaction system utilizing


medium fluorous-tag CH/  interaction

Development of novel fluorous coupling


Synthesis and RCM reactions using reagents for a concise condensation reaction
recyclable Grubbs-Hoveyda metathesis
catalyst

Effective synthesis of biologically active


Novel and efficient methodology for flavones via a cesium enolate assisted
radical cyclization in water intramolecular ipso-substitution reaction

Fulorous mixture synthesis of Direct perfluoroalkylation of non-activated


biologically active peptides aromatic C-H bonds of phenols

New synthetic methods

Novel use of performance materials for biocatalyzed reaction Design of a new solid-phase catalyst utilizing polymer-
silica hybrid materials
Catalyst
species

Ionic polymer
chain

Si
Si
Si O O O OO O
OH
OH O O O OH OH
OH

Development of new organic reaction system utilizing


ionic polymer gel

Development of molecular catalysts and new synthetic


methods utilizing biofunctions.

Design and synthesis of new functional molecules and


(polymer chain) materials.

Recent publications:
・Matsugi, M. et al. (2013) A non-enzymatic kinetic resolution of (±)-trans-2-arylcyclohexanols via esterification using polymer-supported DCC, DMAP, and 3β-acetoxyetienic acid. Synth. Commun. in press.
・Hamamoto, H. (2012) Development and application of new oxidation systems utilizing oxo-metalate catalysts. Chem. Pharm. Bull. 60, 799-817.
・Hamamoto, H. et al. (2012) Synthesis of pyrrolophenanthridone alkaloid kalbretorine from indolecarboxylic acids via hypervalent iodine(III) mediated halodecarboxylation and reduction. Tetrahedon Lett. 53, 1924-1927.
・Matsugi, M. et al. (2012) A fluorous Mukaiyama coupling reagent for a concise condensation reaction: Utility of medium-fluorous strategy. Tetrahedron, 68, 3885-3892.
・Hamamoto, H. et al. (2011) Hypervalent Iodine(Ⅲ)/LiX Combination in Fluoroalcohol Solvent for Aromatic Halogenation of Electron-Rich Arenecarboxylic Acids. Synlett, 11, 1563-1566.
・Hamamoto, H. et al. (2011) Simple Synthesis of Pratosine and Hippadine by Intermolecular Palladium-Catalyzed Cycrization and Decarboxylation. Heterocycles, 83, 1111-1119.
・Matsugi, M. et al. (2010) Synthesis and RCM reactions using a recyclable Grubbs-Hoveyda metathesis catalyst activated by a light-fluorous tag. J. Org. Chem., 75, 7905-7908.
・Matsugi, M. et al. (2010) An effective synthesis of 5, 4’-disubstituted flavones via a cesium enolate assisted intramolecular ipso-substitution reaction. Chem. Pharm. Bull. 58, 1107-1110.
・Matsugi, M. et al. (2010) Pronounced rate enhancements in condensation reactions attributed to the fluorous tag in modified Mukaiyama reagents. Tetrahedron Lett. 51, 133-135.
・Hamamoto, H. et al. (2010) Hypervalent Iodine(Ⅲ) Mediated Decarboxylative Halogenation of Indolecarboxylic Acids for the Synthesis of Haloindole Derivatives. Synlett, 11, 1563-1566.
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Department of Applied Biological Chemistry

Laboratory of Pesticide Chemistry


STAFF Associate Professor Yoshihiko OBARA
Pesticide Chemistry I & II
TEACHING Cell Control of Organic substance I & II Associate Professor
Yoshihiko OBARA

Research

Agrochemicals and their related fields.

Development of
novel pesticides

Agrochemicals
(Registration agrochemicals)
Application of protection of crops by using Mode農薬の
of action of

(     )
pesticides fungicides, insecticides, 作用機構
pesticide
herbicides,
plant growth regulators etc.

Pesticides residue in food


食品への農薬の残留
Environmental pollution caused
環境中への放出と蓄積
by pesticides and
its accumulation in soil

Theme for research in our laboratory

●Biological activity of heterocyclic compounds

●Metabolism of compounds causing blindness

●Search of bioactive substances from actinomyces

●Synthesis of azo-benzens and its utilization

Recent publications:
・A. Sengupta and Y. Obara et al. (2002) Induction of blindness by formoguanamine hydrochloride in adult male roseringed parakeets (Psittacula krameri) J. Biosci. 27, 687-693.

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Department of Environmental Bioscience

Laboratory of Plant Conservation Science


STAFF Professor Guang-Xi WANG Lecturer Shigeru YOKOUCHI
Conservation Plant Science Taxonomy of Seed Plant
TEACHING Environmental Stress Biology Plant Systematic and Morphology
Advanced Plant Conservation Science (MC) Professor Lecturer
Guang-Xi WANG Shigeru YOKOUCHI

Research
Focusing on plant species diversity and their environmental adaptabilities for conservation purpose
Aichi Prefecture, Japan, has a markedly diverse natural environment, with remote mountains, alluvial plains, wetlands
and moors, rivers, lakes, marshes and coastal areas, which are home to many species of wild plants. Many of these
species are now threatened with extinction due mainly to human activities. We see this varied environment with its plant
species, as a precious and irreplaceable resource, the protection and conservation of which is our responsibility. Our
laboratory explores the relationships between the diversity of wild plant species and environmental changes, focusing
on threatened plant species, using taxonomical, ecological and genetic methods. Additionally, we also study plants in
agricultural and urban ecosystems, scaled from plant genes, to individuals, populations, communities and ecosystems,
in order to contribute to their conservation through promoting research of plant diversity.

Reproductive ecology of Monochoria korsakowii, an endangered species

Note the position of the white pistil and dark purple anther. This “mirror-image dimorphism” in the flowers of Monochoria
korsakowii promote outcrossing. It should have an effect on the spreading of a herbicide-resistant gene that has developed
in this species in the recent years.

Taxonomic study of the endangered species in Camellia.

Many of Camellia species native to Vietnam and Laos are now threatened to extinction. The phylogenetic and taxonomic
studies are conducted in our laboratory.

Our range of scope (in addition to above-shown subjects)

Research sites: Research fields: Species under research:


Arable land, Taxonomy, Drosera peltata, Sagittaria aginshi, Sagittaria
urban area, physiological ecology, trifolia, Bidens frondosa, Monochoria vaginalis,
and Andropogon virginicus, Eriocaulon parvum etc.
lawn etc.
conservation botany
Recent publications:
・Shito, Y., H. Usami, A. M. Iwakawa, S. Yokouchi, T. Takeshita and H. Saka. 2010. Time-dependent ethylene performance and its seasonal fluctuation in excised leaf discs of various plant species. Scientific Reports of the Faculty of
Agriculture, Meijo University 46: 19-25.
・Furuhashi, K., Yokouchi, S., Saka, H. (2010) Establishment of in vitro culture system which permits to select parastic ability deficient mutants in Cuscuta campestris, a holoparasitic flowering plant. Scientific Reports of the Faculty
of Agriculture, Meijo University 46: 51-56.
・Kohara, H., X.-C. Wan, K. Akai and G.-X. Wang. 2011. Weed Monograph 7: Monochoria korsakowii Regel et Maack. Journal of Weed Science and Technology 56: 166-181.
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・Liu,S., Y. Xue, X., Wang, B. Zhang, Y. Bi, M .Qiu, G.-X.Wang and P.Wu (2011) A dominant mutation in ARL2 causes impaired adventitious root development in rice.J.Plant Biol. 54, 227-236.
・Yoshino, N., G.-X. Wang, A. Uchino and T. Tominaga (2011) Hybridizations and genetic relationships among Lindernia species (Scrophulariaceae): L. procumbens and two subspecies of L. dubia. Aquatic Botany 94, 165-171. Back
Department of Environmental Bioscience

Laboratory of Environmental Zoology


STAFF Professor Teruaki HINO Associate Professor Yasuaki NIIZUMA
Environmental Zoology Animal Ecology and Conservation
Ecology Biometrics
TEACHING
Biometrics Environmental Bioscience III
Advanced Animal Ecology (MC) Advanced Animal Ecology (MC) Professor Associate Professor
Teruaki HINO Yasuaki NIIZUMA

Research

Research on relationship between animals and their environments

seabirds Freshwater
fish

Influence of soil runoff on marine ecosystem Influence of foraging on river ecosystem

deer cormorants

Influence of grazing on forest ecosystem Influence of feces on satoyama ecosystem

forest birds ground beetles frogs

Our laboratory examines the ecology, behavior and physiology of various animals
living in their natural environment ranging from the ocean to the land. Analyzing
the interactions between organisms in the same or different ecosystems helps us
understand how biodiversity has been maintained and must be conserved.

Recent publications:
・Hino T, Ishida A (2012) Home ranges and seasonal movements of Great Cormorants Phalacrocorax carbo in the Tokai area, based on GPS-Argos tracking. Japanese Journal of Ornithology 61: 17-28.
・Ito H, Hino T, Sakuma D. (2012) Species Abundance in Floor Vegetation of Managed Coppice and Abandoned Forest. Forest Ecology and Management 269: 99-105.
・ Furusawa H, Hino T, Kaneko S, Araki M. (2011) The effects of understory grazing by deer on aboveground N input to soil and soil N mineralization in a forest on Mt. Ohdaigahara in Japan. Japanese Journal of Forest
Environment 53: 1-8.
・Niizuma Y (2011) Relationship between body water and lipid in three seabird species: Leach’s Storm-Petrels, Rhinoceros Auklets and Black-tailed Gulls. Ornithological Science 10: 35-38.
・Sato T, Watanabe K, Kanaiwa M, Niizuma Y, Harada Y, Lafferty K (2011) Nematomorph parasites drive energy flow through a riparian ecosystem. Ecology 92: 201-207
・Kazama K, Niizuma Y, Sakamoto KQ, Watanuki Y (2011) Factors affecting individual variation in nest defense intensity in colonially breeding Black-tailed Gulls. Canadian Journal of Zoology 89: 938-944.

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Department of Environmental Bioscience

Laboratory of Environmental Analytical Chemistry


STAFF Associate Professor Takeshi OHURA
Analytical Chemistry
Environmental Analytical Chemistry
TEACHING
Instrumental Analysis
Advanced Material Cycling Systems (MC) Associate Professor
Takeshi OHURA

Research
MOLECULAR EVIDENCES OF ENVIRONMENTAL CHEMICALS
Environmental contaminants and sustainable materials

 Environmental behaviors
- Exploration of environmental contaminants -

The collected air pollutants are analyzed by


Collection from sediment using HPLC and GC/MS, suggesting their
and air samples distribution and sources.

 Biological effects
- Risks of exposure to environmental contaminants -
Bioassay using recombinant yeast

3200
BaP
3-ClFluor
-gal activity (U)

2400 1-ClPy
7-ClBaA
1600
6-ClBaP

800

Cl 0
0.0001 0.001 0.01 0.1 1 10 100
M

Structure-activity correlation between AhR activities


Toxic assessments of environmental pollutants using and molecular surface areas of chemicals
mice and human cells

 Development of low environmental burden & functional


materials - Efficient use of biomass -
SEM of nano-cellulose fibers

Original Functional
cellulose

Biosynthesis of biodegradability polymers Novel construction of functional bio-based materials


(biopolymer showing yellow dots in cells)

Recent publications:
・ Ohura T, Morita M, Kuruto-Niwa R, Amagai T, Sakakibara H, Shimoi K. (2010) Differential action of chlorinated polycyclic aromatic hydrocarbons on aryl hydrocarbon receptor-mediated signaling in breast cancer cells.
Environ. Toxicol., 25, 180-187.
・Ohura T, Sawada K, Amagai T, Shinomiya M. (2009) Discovery of novel halogenated polycyclic aromatic hydrocarbons in urban particulate matters: Occurrence, photostability, and AhR activity. Environ. Sci. Technol., 43, 2269-2275.
・Ohura T. (2009) "Chemical characterization of ambient particle: PAHs and chlorinated PAHs", In Airborne Particulates (M. Cheng & W. Liu, Eds.), NOVA Science Publishers, NY, pp 157-170.
・Ohura T, Fujima S, Amagai T, Shinomiya M. (2008) Chlorinated polycyclic aromatic hydrocarbons in the atmosphere: Seasonal levels, gas-particle partitioning, and origin. Environ. Sci. Technol., 42, 3296-3302.
・Ohura T, Morita M, Makino M, Amagai T, Shimoi K. (2007) Aryl hydrocarbon receptor-mediated effects of chlorinated polycyclic aromatic hydrocarbons. Chem. Res. Toxicol., 20, 1237-1241.
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Department of Environmental Bioscience

Laboratory of Environmental Soil Science


STAFF Professor Toshiyuki ISOI Assistant Professor Hirotatsu MURANO
Material Cycling Systems Soil Science
Fertilizer Science Environmental Soil Science
TEACHING
Advanced Material Cycling Systems (MC)
Advanced Soil Science (MC) Professor Assistant Professor
Toshiyuki ISOI Hirotatsu MURANO

Research
We research the material cycle of soil, plant and symbiotic
microorganisms to contribute to the environment and society.
To establish low-input farming systems, we We examine the material cycles in
investigate the behavior of nutrients in soil, ecosystems and farmlands using
focusing on chemical speciation, enzyme stable isotopes.
activity, organic matter contents, arbuscular
mycorrhizal fungi and rhizobium.

We aim at developing remediation We examine the symbiotic interactions between plants and
method of the contaminated soils arbuscular mycorrhizal fungi or rhizobium by using the
by studying the bioavailability of biochemical and molecular biological method and try to
inorganic and organic pollutants in improve the level of understanding about the degree to
soil. which these microorganisms contribute to plant growth.
Recent publications:
・Isoi, T. and M. Habte (2012) Reactions of sun hemp (Crotalaria juncea L.) to phosphorus concentration in soil solution, nitrogen fertilization, and arbuscularmycorrhizal colonization. Scientific Reports of the Faculty of
Agriculture, Meijo University, 48:7-12.
・Seike, N., M. Sakai, H. Murano, M. Okamoto, T. Saito, I. Narita, Y. Hashimoto, Y. Ikeda, M. Endo, T. Otani (2012) Relationship between dieldrin uptake in cucumber (Cucumissativus) fruits growing in fields and 50% (v/v)
methanol-water extractable residue in soil. Journal of Pesticide Science, 37 (3): 252-257.
・Maejima, Y., H. Murano, T. Iwafune, T. Arao and K. Baba (2011) Adsorption and mobility of aromatic arsenicals in Japanese agricultural soils. Soil Science & Plant Nutrition, 57 (3): 429-435.
・Ozaki, K. and Isoi, T. (2010) Estimation of rhizobial population in soil by a competitive PCR method. Japanese Journal of Soil Science and Plant Nutrition, 81: 44-47.
・Murano, H., T. Otani and N. Seike (2010) Dieldrin-dissolving abilities of the xylem saps of several plant families, particularly Cucurbitapepo L. Environmental Toxicology and Chemistry, 29(10): 2269-2277.
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Department of Environmental Bioscience

Laboratory of Plant Physiology and Function


STAFF Professor Toru FUNAGUMA Associate Professor Ayumu KONDO
Biological Chemistry Plant Biochemistry
Plant Nutrition Plant Physiology
TEACHING
Plant Functional Science Advanced Plant Environmental Physiology (MC)
Advanced Plant Environmental Physiology (MC) Professor Associate Professor
Toru FUNAGUMA Ayumu KONDO

Research
Elucidation of the environmental
adaptation mechanisms in plants
Response mechanism of plant male
gametophyte to nutrient stress
Plant male gametophyte, pollen plays an important role such as bridging life from
the past to the future on earth. Mature pollen released from anther is extremely dry,
and in a state of dormancy. However, when pollen absorbs water, it germinates and
subsequently elongates the pollen-tube for fertilization.
Mature pollen (photo 1) from Pinus thunbergyi germinates in about 20 h on agar
medium and then significantly elongates the pollen-tube after 48 h-cultivation
(photo 2). We found an interesting phenomenon that the pollen-tube growth is
strongly suppressed even after 48h-cultivation with the addition of a natural
substance to this medium (photo 3). Currently, to clarify the regulatory mechanism
of pollen-tube elongation of pinus pollen, we have biochemically tried to elucidate
the inhibition mechanism of pollen-tube elongation by the natural substance.

Active oxygen scavenging


mechanism in plants
When plants encounter stressful environment, active oxygen is generated in its
Clumped chloroplasts cell. Since the strong oxidizing power of active oxygen may cause various
obstacles to living cells, it is thought that the plant uses the defense and
Chloroplast
response mechanism using antioxidants.
In this laboratory, it was found that the clumping of chloroplast was caused by
both factors of light and water stress in several sorts of succulent plants (photo
1, control; photos 2, 3, water stress). The optical transmittance of the leaf
increased 3-fold compared with the control by forming the clumping of
Clumped Chloroplasts
chloroplasts. The results suggested that such a phenomenon plays an
important role as one of the evasion mechanisms of the optical obstacle
resulting from water stress. Further analysis is required to know more about
the relationship between the clumping phenomenon of chloroplasts and active
oxygen scavenging enzyme systems including ascorbic acid peroxidase.

Carbon metabolism mechanism


of CAM plants
Chloroplast

Nucleus
CAM (Crassulacean Acid Metabolism) represents one of the three major
photosynthetic carbon metabolisms, together with C3 and C4 pathways. CAM
plants have drought tolerance that enables plants to survive in arid areas.
Therefore, the features of CAM plants have been attracting for use in preserva-
tion of greenery and prevent desertification. Recent studies reveal that the
Chloroplasts clumped around the nuclei. intracellular localization of a key enzyme in carbon metabolism, pyruvate, Pi
dikinase (PPDK) in mesophyll cells of CAM plants varies among species, and
connected with that, the pathways in decarboxylation of malic acid are also
different. These findings show that CAM system is more diverse than
previously thought. Further detailed analysis is necessary to know the behavior
of intermediate on responses to environmental stress. Our goal is to clarify the
metabolic pathway and its regulation in CAM to apply the useful features of
CAM plants to crop production under water deficit conditions.

Data analyzed by use of HPLC

Some CAM plants. (Pineapple, Zygocactus, Kalanchoe showed from the left.)

Recent publications:
・Kondo A., Murakami H. Y. and Funaguma T. (2010) Induction of CAM by salt stress in the common purple ice plant, Lampranthus spectabilis. J. Res. Inst. Meijo Univ., 9:11-17
・ Kondo A, Shibata K, Sakurai T, Tawata M, Funaguma T. (2006) Intracellular positioning of nucleus and mitochondria with clumping of chloroplasts in the succulent CAM plant Kalanchoë blossfeldiana: an investigation
using fluorescence microscopy. Plant Morphology, 18: 69-73
・Kondo A, Kaikawa J, Funaguma T, Ueno O. (2004) Clumping and dispersal of chloroplasts in succulent plants. Planta, 219: 500-506
・Kondo A, Nose A, Ueno O. (2001) Coordinated accumulation of the chloroplastic and cytosolic pyruvate, Pi dikinases with enhanced expression of CAM in Kalanchoë blossfeldiana. Physiol. Plant., 111: 116-122 19
・Funaguma T, Okabayashi T, Horii T, Hara A. (1999) A glycosidase with β - D - glucosidase and β - D - fucosidase from pollen of Typha latifolia. Jpn. J. Palynol., 45: 159-163
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Department of Environmental Bioscience

Laboratory of Environmental Microbiology


STAFF Professor Hiroto TAMURA Associate Professor Akifumi HOSODA
Environmental Bioscience I Microbiology
Environmental Microbiology Environmental Bioscience II
TEACHING Molecular Biology Advanced Science in Bioremediation (MC)
Environmental Toxicology (MC) Professor Associate Professor
Advanced Science in Bioremediation (MC)
Hiroto TAMURA Akifumi HOSODA

Research

Taking up the challenge for environmental remediation


through Cutting-edge analytical approaches & Biotechnology
@ Mechanism of metabolic toxicity by microbial degradation
* Environmental dynamics of alkylphenol * Molecular phylogenetic analysis based on
& Isolation of APEOn degrading bacteria novel identification & discrimination method
- S10-GERMS method -

Gas Chromatography (GC)


Matrix-Assisted Laser Desorption Ionization mass spectrometry Electron micrograph of
(MALDI-MS) Phylogenetic analysis based on S10-GERMS method
surfactant-degrading bacteria
Gas Chromatography mass spectrometry (GC-MS)

@ Biodegradation of environmental pollutants to Biorefinery


* Biorefinery of wastes & pollutants * Dechlorination of chlorinated volatile organic
 compounds by anaerobic bacteria
(a)

(b)

Growth chamber for anaerobes


Generation of renewable energy Our goal is to be No.1 (a) Microtip electrophoresis
by GET system Electron micrograph of
oil-degrading bacteria
on solid surface
eco-friendly laboratory !! (b) Analysis of microbial diversity
by Denature gradient gel electrophoresis

* Wastewater treatment @ Investigation of environmental compounds


disrupting normal endocrine function
F e 2+ c o n c
100
90
80
70
60
F e 2+ m g/L

50

* Analysis of Androgen (Glucocorticoid) receptor


40
30
20
10

agonist and/or antagonist activity of chemicals


0
0 10 20 30 40 50
h o u rs

Fe-oxidizing bacteria
Decrease of Fe2+ ion
on Fe3+ particles
Fe-oxidizing
bacteria

Let’ s find scientific solutions


to environmental issues at
our Laboratory ! Proteome analysis Real-time PCR

Recent publications:
・Hotta, Y, H. Sato, A. Hosoda, H. Tamura, (2012) MALDI-TOF MS analysis of ribosomal proteins coded in S10 and spc operons rapidly classified the Sphingomonadaceae as alkylphenol polyethoxylate-degrading bacteria from the
environment., FEMS Microbiol. Lett. in press
・Hosoda, A., T. Takahashi, K. Numano, K. Nakajou, A. Higashimoto, M. Toda, H. Arai, Y. Hotta, H. Tamura, (2012) Rapid reductive dechlorination of trichloroehtene in contaminated ground water using biostimulation agent, BD-1,
formulated from canola oil., J. Oleo Sci. 61: 155-161.
・Hotta, Y, J. Sato, H. Sato, A. Hosoda, H. Tamura, (2011) Classification of the genus Bacillus based on MALDI-TOF MS analysis of ribosomal proteins coded in S10 and spc operons. J Agric Food Chem. 59: 5222-5230.

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Department of Environmental Bioscience

Laboratory of Landscape Design


STAFF Professor Hiroshi MARUYAMA Assistant Professor Hiroshi HASHIMOTO
Landscape Design Landscape Ecology and Management
Public Open Spaces Garden Materials and Planting
TEACHING
Advanced Landscape Design (MC)
Advanced Theory of Public Open Spaces (MC) Professor Assistant Professor
Hiroshi MARUYAMA Hiroshi HASHIMOTO

Research

Landscape Design
The field of present-day landscape design covers not only private
gardens, urban parks and open spaces in urban areas, but also
Satoyama in suburb, National Parks etc. Our laboratory studies
about these areas from various perspectives.

Urban Greening
Park, Open space, Roadside trees, Promenade

History & Culture of Greenery


Shrine forest, Satoyama, Japanese Garden

Amenity of Greenery
Recreation, Landscape, Scenic beauty

Harmony with Nature


Nature conservation, Biotope, National park

Environmental Education
Civic participation, Recycle

To design any landscapes, we need to have basic


design skill and knowledge of plants. In addition,
we must also understand the history, culture and
environment of that particular region.
Our faculty will provide you with various lectures,
experiments and practices to widen your vision.
Recent publications:
・Maruyama, H.et al. ed. (2008) Modern Kyoto Studies. Kyoto: Shibunkaku publishing company.
・Maruyama, H. et al. ed. (2007) Modern Age of ' Miyako(Kyoto)', Shibunkaku publishing company.
・Maruyama, H. (2006) Landscape Architecture in Exposition. (in) Morimoto, Y. and Shirahata, Y. ed. Environmental Design. Asakura publishing company.
・Hashimoto, H. et al. (2012) Characteristics and Long-term Trends of the Avifauna of Lake Biwa. (in) Kawanabe, H. et al. ed. Lake Biwa: Interactions between Nature and People. Springer.
・Natuhara, Y. and Hashimoto, H. ( 2009) Spatial pattern and process in urban animal communities. (in) McDonnell, M. J., ed. Ecology of Cities and Towns: A Comparative Approach. Cambridge University Press.
・Hashimoto, H. (2007) Connectivity analyses of avifauna in urban areas. (in) Hong, S. K. et al. ed. Landscape Ecological Applications in Man-Influenced Areas: Linking Man and Nature Systems. Springer.
・Hashimoto, H. et al. (2005) A habitat model for Parus major minor using a logistic regression model for the urban area of Osaka, Japan. Landscape and Urban Planning, 70: 245-250.

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Experimental Farm
http://www-agr.meijo-u.ac.jp/labs/nn023/
Professor Shoji TSUCHIYA Associate Professor Yoshinori NAKAO
STAFF Associate Professor Takashi MORITA
Associate Professor Yoshiaki HAYASHI
Instructor Yoshiharu KATAYAMA Professor Associate Professor Associate Professor
Agricultural Practice A, B, C, D, E (for Department of Agrobiological Resources) Shoji TSUCHIYA Yoshinori NAKAO Takashi MORITA
Agricultural Practice (for Department of Applied Biological Chemistry)
TEACHING Agricultural Practice (for Department of Environmental Bioscience)
Special Topics in AgricultureⅡ
Special Topics in Agriculture Ⅲ

Associate Professor Technical Assistant


Yoshiaki HAYASHI Yoshiharu KATAYAMA

Laboratory of Plant and Animal Science


䞉 The quality and growth of spring, summer and autumn crops.
䞉 Studies of the cultivation density and growth between spring and
Crop Science summer cropping potato.
䞉 Studies of garlic production.

䞉 Fruit growth and maturation control by the plant growth regulator


treatment.
Pomology 䞉 Studies of the response mechanism of fruits to environmental changes.
䞉 Studies of the functional ingredients in fruits.

䞉 Studies on the evaluation and utilization of companion plants.


䞉 Studies on malformation and sunburn of paprika fruits.
Vegetable science
䞉 Studies on production, storage and utilization of taro.

䞉Studies on the growth and flowering of bedding plants.


Floricultural science 䞉Studies on the production of bulbs, corms, tubers and tuberous roots.

䞉 Studies on effective utilization of unused resources in poultry


production and immunity.
Animal Science 䞉 Studies on goat utilization for reclamation of abandoned land and
dairy production.
䞉 Studies on effective utilization of unused resources with manure for
composting.
䞉 Studies on improvement of livestock production in tropical areas.

Cultivation of Stone cell of Mixed planting of


various rice spices. Japanese pear. cabbage and chamomile
(companion plants).

Improved growth of Impatiens Research on growth


in lower soil-temperature. of buffaloes in Nepal.
Recent publications:
・Hayashi Y., Shah M. K., Tabata Y., Kumagai H., Shah S. K. and Devkota N. R. (2011) Feeding characteristics and body dimensions of growing buffaloes (Bubalus bubalis) raised on small-scale farms in Tarai, Nepal. Journal of
Institute of Agriculture and Animal Science. 32,181-186.
・Hayashi Y., Shah M. K., Kumagai H. and Shah S. K.(2010) Comparison of bodyweight and body size of growing buffaloes between large and small scale farms in Nepal. Revista Veterinaria. 21, 781-783.
・Hayashi Y., Shah M. K., Kumagai H. and Shah S. K.(2009) Body weight and body size of growing buffaloes raised in Nepal. 2009. Pakistan Journal of Zoology. 9, 143-145.
・Hayashi Y., Thapa B. B., Sharma M. P., Sapkota M. and Kumagai H.(2009) Effects of maize (Zea mays L.) silage feeding on dry matter intake and milk production of dairy buffalo and cattle in Tarai, Nepal. Animal Science Journal. 80, 418-427.
・Nakao, Y., T. Taira, S. Horiuchi, K. Kawase, and Y. Mukai(2005) Chromosomal Difference between Male and Female Trees of Ginkgo biloba Examined by Karyotype Analysis and Mapping of rDNA on the Chromosomes by
Fluorescence in situ Hybridization. J. Japan. Soc. Hort. Sci. 74, 275-280. 22
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  Curriculum of Faculty of Agriculture, Meijo University in 2012
Curriculum for Bachelor's Degree
Department of Agrobiological Resources Department of Applied Biological Chemistry Department of Environmental Bioscience

Biology Mathematics Biological Experiments Scientific English I


Chemistry Information Science Chemical Experiments Scientific English II
Physics Geology Internship I Internship II
Special Topics in Agriculture I Special Topics in Agriculture II Special Topics in Agriculture III Overseas Language Study
Introduction to Agrobiological Resources Laws on Food Rural Areas & Environment Taxonomy of Seed Plant
Crop Production Science Statistics Biological Chemistry
Horticultural Science Analytical Chemistry Inorganic Chemistry
Food Crops Science Instrumental Analysis Environmental Bioscience I
Biological Chemistry Agricultural Practice Analytical Chemistry
Genetics Food Processing Practice Environmental Bioscience II
Plant Pathology Public Hygiene Biometrics
Fundamental Entomology Plant Science Introduction to Environmental Bioscience
Management of Agrobioproduction Inorganic Chemistry I Conservation Plant Science
Research Methods of Life Science Inorganic Chemistry II Ecology
Laboratory works in Agrobiological Resources Organic Chemistry I Environmental Zoology
Agricultural Practice A Organic Chemistry II Animal Ecology and Conservation
Agricultural Practice B Biological Chemistry I Wildlife Management and Conservation
Laboratory Works in Crop Science Biological Chemistry II Environmental Stress Biology
Laboratory Works in Horticultural Science Physical Chemistry I Forest Ecology
Laboratory Works in Genetics and Breeding Science Physical Chemistry II Landscape Design
Seminar in Agrobioproductional Management Metabolic Biological Chemistry I Landscape Ecology and Management
Laboratory Works in Plant Pathology Metabolic Biological Chemistry II Public Open Spaces
Laboratory Works in Entomology Molecular Biology Garden Materials and Planting
Undergraduate Research Biotechnology Ecology of Hymenomycetes
Seminar I Microbiology I Environmental Law
Seminar II Microbiology II Environmental Impact Assessment
Agricultural Practice C Microbial Technology I Plant Biochemistry
Agricultural Practice D Microbial Technology II Plant Nutrition
Agricultural Practice E Cell Technology Plant Physiology
Plant Systematic and Morphology Protein Engineering Plant Functional Science
Zoological Systematic and Morphology Nutritive Science I Material Cycling Systems
Microbiology Nutritive Science II Soil Science
Agricultural and Resource Economics Food Chemistry Environmental Soil Science
Statistics of Agricultural Science I Food Functionality Fertilizer Science
Statistics of Agricultural Science II Food Resources I Environmental Analytical Chemistry
Pomology Food Resources II Instrumental Analysis
Vegetable Crop Science Food Science & Technology I Hydrosphere Environmental Chemistry
Floricultural Science Food Science & Technology II Environmental Bioscience III
Breeding Science Food Preservation Environmental Microbiology
Breeding Science Food Hygiene Molecular Biology
Environmental Control in Crop Growth and Development Food Palatability I Laboratory Works in Environmental Bioscience I
Soil Science Food Palatability II Laboratory Works in Environmental Bioscience II
Fertilizer Science Food Habits Landscape Design Practice
Plant Resources Bio-organic Chemistry I Seminar I
Tropical Agriculture Bio-organic Chemistry II Seminar II
Weed Science Cell Control of Organic substance I Undergraduate Research
Organic Chemistry Cell Control of Organic substance II Practice in Environmental Bioscience
Metabolic Physiology Pesticide Chemistry I Special Topics in Environmental Bioscience I
Plant Physiology Pesticide Chemistry II Special Topics in Environmental Bioscience II
Cell Technology Organic Natural Product Chemistry Special Topics in Environmental Bioscience III
Cell Biology Bio-physical Chemistry Agricultural Practice
Molecular Biology Chemical Engineering
Agroenvironmental Microbiology Introduction to Applied Biological Chemistry *Bold letter is required subject.
Plant Disease Control Seminar I
Applied Entomology Seminar II
Pesticide Science Laboratory Works in Biological Chemistry I
Agricultural Policy Laboratory Works in Biological Chemistry II
Food System Economics Laboratory Works in Biological Chemistry III
Fundamentals of Animal Production Laboratory Works in Biological Chemistry IV
Animal Production Science Laboratory Works in Biological Chemistry V
Special Topics in Agrobiological Resources I Laboratory Works in Biological Chemistry VI
Special Topics in Agrobiological Resources II Laboratory Works in Biological Chemistry VII
Special Topics in Agrobiological Resources III Undergraduate Research
Special Topics in Applied Biological Chemistry I
*Bold letter is required subject. Special Topics in Applied Biological Chemistry II
Special Topics in Applied Biological Chemistry III

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  Curriculum of Faculty of Agriculture, Meijo University in 2012
Common Liberal Curriculum
Subjects for Human and Culture Subjects for Languege and Communication
History and Culture English(Elementary)(Reading)I German Oral Communication(Basic)I
Literature and Human Beings English(Elementary)(Communication)I German Oral Communication(Basic)II
Aesthetics English(Elementary)(Reading)II German Oral Communication(Intermediate)I
Area Studies (Europe and America) English(Elementary)(Communication)II German Oral Communication(Intermediate)II
Area Studies (Asia) English(Pre-intermediate)(Reading)I Chinese(Beginner)I
Cultural Anthropology English(Pre-intermediate)(Communication)I Chinese(Beginner)II
Psychology English(Pre-intermediate)(Reading)II Chinese(Basic)I
Religion English(Pre-intermediate)(Communication)II Chinese(Basic)II
Philosophy English(Intermediate)(Reading)I Chinese(Intermediate)I
English(Intermediate)(Communication)I Chinese(Intermediate)II
Subjects for Human and Society English(Intermediate)(Reading)II Chinese Oral Communication(Basic)I
Japanese Constitution English(Intermediate)(Communication)II Chinese Oral Communication(Basic)II
Low and Society English(Advanced)(Reading)I Chinese Oral Communication(Intermediate)I
Economy and Society English(Advanced)(Reading)II Chinese Oral Communication(Intermediate)II
Management and Society English(Advanced)(Communication)I Korean(Beginner)I
Politics and Society English(Advanced)(Communication)II Korean(Beginner)II
Humans & Society in the Age of Internationalization English(Basic)(Reading)I Korean(Basic)I
Sociology English(Basic)(Communication)I Korean(Basic)II
Urban Society and Human Beings English(Basic)(Reading)II Korean(Intermediate)I
Gender and Society English(Basic)(Communication)II Korean(Intermediate)II
Seminar on Humans and Society French(Beginner)I Korean Oral Communication(Basic)I
French(Beginner)II Korean Oral Communication(Basic)II
Subjects for Nature and Envirnment French(Basic)I Korean Oral Communication(Intermediate)I
Introduction to Earth & Planetary Sciences French(Basic)II Korean Oral Communication(Intermediate)II
Introduction to Physics French(Intermediate)I Spanish(Basic)I
Introduction to Biology French(Intermediate)II Spanish(Basic)II
Introduction to Mathematics French Oral Communication(Basic)I Japanese I*
Introduction to Principles of Science French Oral Communication(Basic)II Japanese II*
Introduction to Environmental Studies French Oral Communication(Intermediate)I Japanese III*
French Oral Communication(Intermediate)II Japanese IV*
Subjects for Computer Operation German(Beginner)I Japanese V*
Computer Literacy German(Beginner)II Outline of Japanese Things I*
Computer Systems German(Basic)I Outline of Japanese Things II*
Application of Computer Literacy German(Basic)II (* : Only for an international student)
Introduction to Data Processing German(Intermediate)I
Introduction to Programming German(Intermediate)II
Moral and Security in Information Society

Subjects for Health and Sport Science


Health and Sport Science I Bold letter is required subject.
Health and Sport Science II
Health and Sport Science III
Health and Sport Science IV
Health Science Theory I
Health Science Theory II
Health and Sport Science V

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POSTGRADUATE PROGRAM OF AGRICULTURE
Curriculum for Advanced Doctral Course of Agriculture Curriculum for Master's Course of Agriculture
Advanced Course in Agrobiological Resources
Plant Production Science
Advanced Crop Production Science
Advanced Crop Physiology
Advanced Studies on Plant Production Sciences Advanced Horticultural Production Science
Advanced Horticultural Physiology
Advanced Seminar in Plant Production Science
Advanced Experiments in Plant Production Science
Genetics
Advanced Genetics and Breeding Science
Advanced Studies on Genetics Advanced Molecular Genetics
Advanced Seminar in Genetics
Advanced Experiments in Genetics
Plant Protection Science
Advanced Entomology and Biodiversity
Advanced Plant Pathology
Advanced Studies on Plant Protection Sciences
Advanced Physiological Plant Pathology
Advanced Seminar in Plant Protection and Biodiversity
Advanced Experiments in Plant Protection and Biodiversity
Agricultural and Resource Economics
Advanced Theory of Farm Management
Advanced Studies on Agricultural & Resource Economics Advanced Theory of Agricultural and Resource Economics
Advanced Seminar in Agricultural and Resource Economics
Advanced Exercises in Agricultural and Resource Economics
Advanced Course in Applied Biological Chemistry
Life Science
Advanced Microbiology
Advanced Studies on Life Sciences Advanced Biological Chemistry
Advanced Seminar in Life Science
Advanced Experiments in Life Science
Food Science
Advanced Nutritional Science
Advanced Food Hygiene
Advanced Studies on Food Sciences Advanced Food Science and Technology
Advanced Functional Food Science
Advanced Seminar in Food Science
Advanced Experiments in Food Science
Bioorganic Chemistry
Advanced Bioorganic Chemistry
Advanced Synthetic Organic Chemistry
Advanced Studies on Bioorganic Chemistry
Advanced Biophysical Chemistry
Advanced Seminar in Bioorganic Chemistry
Advanced Experiments in Bioorganic Chemistry
Advanced Course in Environmental Bioscience
Environmental Bioscience
Advanced Plant Conservation Science
Advanced Studies on Environmental Biosciences Advanced Animal Ecology
Advanced Seminar in Environmental Bioscience
Advanced Experiments in Environmental Bioscience
Dynamic Soil Science
Advanced Soil Science
Advanced Studies on Dynamic Soil Sciences Advanced Material Cycling Systems
Advanced Seminar in Dynamic Soil Science
Advanced Experiments in Dynamic Soil Science
Bioremediation・Environmental Response
Advanced Science in Bioremediation
Environmental Toxicology
Advanced Studies on Bioremediation & Environmental Response
Advanced Plant Environmental Physiology
Advanced Seminar in Bioremediation・Environmental Response
Advanced Experiments in Bioremediation・Environmental Response
Landscape Design
Advanced Landscape Design
Advanced Studies on Landscape Design Advanced Theory of Public Open Spaces
Advanced Seminar in Landscape Design
Advanced Practice in Landscape Design
Common Course in Agricultural Science
Advanced Seminar in Agricultural Science
Advanced Lecture in Agricultural Science I - VI
Advanced Presentation Training
Advanced Training in Agricultural Science I - III

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Meijo University
Graduate School of Agriculture
Faculty of Agriculture

Department of Department of Department of


Agrobiological Applied Environmental
Resources Biological Bioscience
Chemistry

1-501 Shiogamaguchi, Tempaku, Nagoya


468-8502 JAPAN

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