Fluorescence Spectrophotometer

● SPECIFICATIONS ● FUNCTIONS F-7100

Item Description Item Description
Sensitivity Noise: Background S/N 20,000 or above*4 Contour plotting (fluorescence/phosphorescence),
(Raman light of water) Noise: Peak 1,200 or above*5 bird’s eye view
3-dimensional
Minimum sample volume 0.6 mL (in use of standard 10 mm rectangular cell) Readout of EX/EM spectra from contour
measurement
Photometric principle Monochromatic light monitoring ratio calculation Peak detection
Calculation between files (+, –, ×, ÷)
Light source 150 W xenon lamp, self-deozonating lamp house
Fluorescence/phosphorescence/luminescence spectra
Stigmatic concave diffraction grating: 900 Synchronous spectra/repetitive measurement/CAT
lines/mm, F2.2 Excitation spectrum correction (200 to 600 nm)
Monochromator
Brazed wavelength: Excitation side 300 nm, Emission spectrum correction (200 to 600 nm)
emission side 400 nm Excitation longer wavelength spectrum correction
Measuring wavelength range 200 to 750 nm, and zero-order light (500 to 800 nm)
(on both EX and EM) (Expandable up to 900 nm with optional detector) Wavelength scan Emission longer wavelength spectrum correction
Excitation side: 1, 2.5, 5, 10, 20 nm (500 to 800 nm)
Bandpass Note: Sub standard light source (option) is necessary.
Emission side: 1, 2.5, 5, 10, 20 nm
Tracing, scale conversion, graph axis conversion
Resolution 1.0 nm (at 546.1 nm) Smoothing
Wavelength accuracy ±1 nm Calculation between files (+, –, ×, ÷)
30, 60, 240, 1,200, 2,400, 12,000, 30,000, Differentiation (first to fourth order)
Wavelength scan speed
60,000 nm/min Contour plotting (fluorescence/phosphorescence),
Wavelength drive speed 60,000 nm/min 3-dimensional
time bird’s eye view
3D measurement time 3 min*6 scan measurement Readout of time scan/EM spectra from contour
Response from 0 to 98 %: Peak detection
Response Calculation between files (+, –, ×, ÷)
0.002, 0.004, 0.01, 0.05, 0.1, 0.5, 2, 4 s Time scan fluorescence/phosphorescence meas-
Photometric value range –9999 to 9999 urement mode (minimum data interval 1.0 ms)
Data processing unit PC: Windows 7 Phosphorescence attenuation curve measurement
Printer Printer compatible with Windows 7 Time scan Rate calculation
Spectrophotometer: 620 W × 520 D × 300 H mm measurement Tracing, scale conversion, graph axis conversion
Dimensions/weight mode Smoothing
(excluding protrusions)/41 kg
Working temperature 15 to 35 °C, 25 to 80 % (condensation not Calculation between files (+, –, ×, ÷)
Differentiation (first to fourth order)
/humidity allowed, 70 % or less at 30 °C or higher)
Area calculation
Power consumption Quantitative analysis
100, 115, 220, 230, 240 V AC, 50/60 Hz, 380 VA
(spectrophotometer) (fluorescence/phosphorescence/luminescence)
FL Solutions program Standard software Two/three-wavelength calculation
*4 EX 350 nm, Slit 10 nm, Response 4 s Calibration curve (linear, quadratic, cubic,
*5 EX 350 nm, Slit 5 nm, Response 2 s polygonal), factor enterable
*6 EX 200 to 750 nm, Sampling interval 10 nm Photometry mode Peak ratio, peak area, quantization via differentiation
EM 200 to 750 nm, Sampling interval 10 nm Interruption, sample blank measurement, data deletion
* “MICROSOFT”, “WINDOWS” and “EXCEL” are registered trademarks or Calibration curve data correction, calibration
trademarks of Microsoft Corporation in the United States and other countries. curve tracing
Cumulative data averaging
labeled model is available Statistic calculation
Automatic sensitivity measurement function
Pre-scan
Others
Data transport and graph copying to Microsoft Excel
Print preview function
FL Intensity Standardization
File conversion of 3D Scan Data
NOTES 1. A PC set is not supplied as standard equipment.
Is should be prepared separately.

Science Ring
This logo symbolizes Scientific and Analytical instruments of Hitachi High-Tech Group. It is composed with an “S”, standing for
“Science”, our technology core competency, and with a ring that represents close connection we make with our customers.
This “Science Ring” shows how we are committed to create new values by strengthening ties between Science and Society.

The above logo is a registered trademark of Hitachi High-Technologies Corporation in the United States and other countries.

CAUTION: For correct operation, follow the instruction manual when using the instrument.
Specifications in this catalog are subject to change with or without notice, as Hitachi High-Tech Science Corporation continues to develop the latest
technologies and products for its customers.
NOTICE: The system is For Research Use Only, and is not intended for any animal or human therapeutic or diagnostic use.
These data are an example of measurement; the individual values cannot be guaranteed.

●Sales ●Manufacture

www.hitachi-hightech.com/global/science/ www.hitachi-hightech.com/global/hhs/
Head Office Head Office
24-14, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo 105-8717, Japan 24-14, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo 105-0003, Japan

Printed in Japan (H) HTB-E119 2016.1


Increased sensitivity achieved via optimized optical
detection system and ultra-bright Xenon lamp
F-7100 is the evolution of the robust and reliable F-7000
with the latest optical technology and improved
analytical performance.
Enhanced Optical System
• Increased excitation luminance
• Improved emission detection sensitivity
• Optimized signal processing
“Best-in-Class” Analytical Signal-to-Noise
Increased sensitivity 1.5x compared to conventional instruments: > 360 of S/N (P-P) and > 1,200 of S/N (RMS)
Comparison of S/N with conventional instruments
The S/N via Raman scattering of water is com-
pared with conventional instruments*1. In addi-
tion to the 1.5x higher sensitivity, weak signals
are detectable due to very low noise levels. This
is also observed in high-speed scanning, which
is a widely utilized function of the F-7000 series.
*1 Conventional instrument: F-7000 Fluorescence Spectrophotometer
Fluorescence Spectrophotometer
Industry leading lamp lifetime
Light source with 5x*2 longer lifetime compared to conventional instruments – lifetime of 2,500 hours*3 –
With the application of the new Xe lamp and the improved lamp ignition
power source, both luminance and the lamp lifetime were increased.
Conventional Increased lamp lifetime reduces operating Cost-of-Ownership and instru-
instrument ment service time.
*2 Comparison with the service life (recommended replacement interval) of the standard Xe lamp
5x longer lifetime (PN: 650-1500) of F-7000 fluorescence spectrophotometer
*3 Lifetime is usable limit. Guaranty for 500 operating hours or 6 months, whichever shorter.
Model F-7100
Troubles due to mishandling are not within guarantee.
Example of S/N measurement result 500 hours 2,500 hours
The automatic sensitivity (S/N) measurement
via Raman scattering of water demonstrates Comparison of lamp lifetimes
“Best-in-Class” analytical sensitivity.
New and Improved Functions and Features for Enhanced Operability
Additional software functions – Enhanced convenience for routine analysis –
FL intensity standardization to correct for fluorescence intensity variation over time and between instruments.
The variations in the fluorescence intensity over time and between instruments can be corrected. Fluorescence
intensity is affected by changes in lamp brightness, room temperature, optical system, etc. The fluorescence intensity
of the standard sample is measured, and the sample fluorescence intensity is converted to the fluorescence intensity
EM Spectra Calibration Curve
relative to the standard sample. This standardization is also used for the intensity comparison between different
instruments, including the analysis of humic substances in environmental water (conversion to quinine sulfate), the
analysis of chlorophyll in water (conversion to fluorescein), and the specified value for reagent purity (conversion to
quinine sulfate).
Window for fluorescence
FL int.
intensity standardization
Enhanced report output function to support multivariate analyses such as fluorescence fingerprint analysis
❶ ❶ File conversion of 3D scan data
EM WL Collective output of multiple 3D fluorescence spectral data to Excel. Output Rows
Conc. ❷ and Columns can be transposed, if needed.
❷ Applicable to the 3D measurement results of the wavelength data table
Example of High Sensitivity Analysis (Fluorescein) ❸ By registering the wavelengths to be focused in the wavelength data table, only the
An example of the high sensitivity analysis for fluorescein is shown. The F-7100 required wavelengths are exported to the Excel sheet.
detected fluorescence in the order of 1x10-13 mol/L (sub-picomol) compared
with a blank sample (purified water); a useful calibration was obtained in the ❸ Data output of eliminate scattering, Eliminate multi-order scattering
ultra-trace range. For the multivariate analysis of 3D fluorescence spectral data, the setting to
exclude the unnecessary scattered light or the data from the secondary light
region is available.
Window of file conversion of 3D scan data

Increased sensitivity achieved via optimized optical
detection system and ultra-bright Xenon lamp
F-7100 is the evolution of the robust and reliable F-7000
with the latest optical technology and improved
analytical performance.
Enhanced Optical System
• Increased excitation luminance
• Improved emission detection sensitivity
• Optimized signal processing
“Best-in-Class” Analytical Signal-to-Noise
Increased sensitivity 1.5x compared to conventional instruments: > 360 of S/N (P-P) and > 1,200 of S/N (RMS)
Comparison of S/N with conventional instruments
The S/N via Raman scattering of water is com-
pared with conventional instruments*1. In addi-
tion to the 1.5x higher sensitivity, weak signals
are detectable due to very low noise levels. This
is also observed in high-speed scanning, which
is a widely utilized function of the F-7000 series.
*1 Conventional instrument: F-7000 Fluorescence Spectrophotometer
Fluorescence Spectrophotometer
Industry leading lamp lifetime
Light source with 5x*2 longer lifetime compared to conventional instruments – lifetime of 2,500 hours*3 –
With the application of the new Xe lamp and the improved lamp ignition
power source, both luminance and the lamp lifetime were increased.
Conventional Increased lamp lifetime reduces operating Cost-of-Ownership and instru-
instrument ment service time.
*2 Comparison with the service life (recommended replacement interval) of the standard Xe lamp
5x longer lifetime (PN: 650-1500) of F-7000 fluorescence spectrophotometer
*3 Lifetime is usable limit. Guaranty for 500 operating hours or 6 months, whichever shorter.
Model F-7100
Troubles due to mishandling are not within guarantee.
Example of S/N measurement result 500 hours 2,500 hours
The automatic sensitivity (S/N) measurement
via Raman scattering of water demonstrates Comparison of lamp lifetimes
“Best-in-Class” analytical sensitivity.
New and Improved Functions and Features for Enhanced Operability
Additional software functions – Enhanced convenience for routine analysis –
FL intensity standardization to correct for fluorescence intensity variation over time and between instruments.
The variations in the fluorescence intensity over time and between instruments can be corrected. Fluorescence
intensity is affected by changes in lamp brightness, room temperature, optical system, etc. The fluorescence intensity
of the standard sample is measured, and the sample fluorescence intensity is converted to the fluorescence intensity
EM Spectra Calibration Curve
relative to the standard sample. This standardization is also used for the intensity comparison between different
instruments, including the analysis of humic substances in environmental water (conversion to quinine sulfate), the
analysis of chlorophyll in water (conversion to fluorescein), and the specified value for reagent purity (conversion to
quinine sulfate).
Window for fluorescence
FL int.
intensity standardization
Enhanced report output function to support multivariate analyses such as fluorescence fingerprint analysis
❶ ❶ File conversion of 3D scan data
EM WL Collective output of multiple 3D fluorescence spectral data to Excel. Output Rows
Conc. ❷ and Columns can be transposed, if needed.
❷ Applicable to the 3D measurement results of the wavelength data table
Example of High Sensitivity Analysis (Fluorescein) ❸ By registering the wavelengths to be focused in the wavelength data table, only the
An example of the high sensitivity analysis for fluorescein is shown. The F-7100 required wavelengths are exported to the Excel sheet.
detected fluorescence in the order of 1x10-13 mol/L (sub-picomol) compared
with a blank sample (purified water); a useful calibration was obtained in the ❸ Data output of eliminate scattering, Eliminate multi-order scattering
ultra-trace range. For the multivariate analysis of 3D fluorescence spectral data, the setting to
exclude the unnecessary scattered light or the data from the secondary light
region is available.
Window of file conversion of 3D scan data
 Fluorescence Spectrophotometer
● SPECIFICATIONS ● FUNCTIONS F-7100
Item Description Item Description
Sensitivity Noise: Background S/N 20,000 or above*4 Contour plotting (fluorescence/phosphorescence),
(Raman light of water) Noise: Peak 1,200 or above*5 bird’s eye view
3-dimensional
Minimum sample volume 0.6 mL (in use of standard 10 mm rectangular cell) Readout of EX/EM spectra from contour
measurement
Photometric principle Monochromatic light monitoring ratio calculation Peak detection
Calculation between files (+, –, ×, ÷)
Light source 150 W xenon lamp, self-deozonating lamp house
Fluorescence/phosphorescence/luminescence spectra
Stigmatic concave diffraction grating: 900 Synchronous spectra/repetitive measurement/CAT
lines/mm, F2.2 Excitation spectrum correction (200 to 600 nm)
Monochromator
Brazed wavelength: Excitation side 300 nm, Emission spectrum correction (200 to 600 nm)
emission side 400 nm Excitation longer wavelength spectrum correction
Measuring wavelength range 200 to 750 nm, and zero-order light (500 to 800 nm)
(on both EX and EM) (Expandable up to 900 nm with optional detector) Wavelength scan Emission longer wavelength spectrum correction
Excitation side: 1, 2.5, 5, 10, 20 nm (500 to 800 nm)
Bandpass Note: Sub standard light source (option) is necessary.
Emission side: 1, 2.5, 5, 10, 20 nm
Tracing, scale conversion, graph axis conversion
Resolution 1.0 nm (at 546.1 nm) Smoothing
Wavelength accuracy ±1 nm Calculation between files (+, –, ×, ÷)
30, 60, 240, 1,200, 2,400, 12,000, 30,000, Differentiation (first to fourth order)
Wavelength scan speed
60,000 nm/min Contour plotting (fluorescence/phosphorescence),
Wavelength drive speed 60,000 nm/min 3-dimensional
time bird’s eye view
3D measurement time 3 min*6 scan measurement Readout of time scan/EM spectra from contour
Response from 0 to 98 %: Peak detection
Response Calculation between files (+, –, ×, ÷)
0.002, 0.004, 0.01, 0.05, 0.1, 0.5, 2, 4 s Time scan fluorescence/phosphorescence meas-
Photometric value range –9999 to 9999 urement mode (minimum data interval 1.0 ms)
Data processing unit PC: Windows 7 Phosphorescence attenuation curve measurement
Printer Printer compatible with Windows 7 Time scan Rate calculation
Spectrophotometer: 620 W × 520 D × 300 H mm measurement Tracing, scale conversion, graph axis conversion
Dimensions/weight mode Smoothing
(excluding protrusions)/41 kg
Working temperature 15 to 35 °C, 25 to 80 % (condensation not Calculation between files (+, –, ×, ÷)
Differentiation (first to fourth order)
/humidity allowed, 70 % or less at 30 °C or higher)
Area calculation
Power consumption Quantitative analysis
100, 115, 220, 230, 240 V AC, 50/60 Hz, 380 VA
(spectrophotometer) (fluorescence/phosphorescence/luminescence)
FL Solutions program Standard software Two/three-wavelength calculation
*4 EX 350 nm, Slit 10 nm, Response 4 s Calibration curve (linear, quadratic, cubic,
*5 EX 350 nm, Slit 5 nm, Response 2 s polygonal), factor enterable
*6 EX 200 to 750 nm, Sampling interval 10 nm Photometry mode Peak ratio, peak area, quantization via differentiation
EM 200 to 750 nm, Sampling interval 10 nm Interruption, sample blank measurement, data deletion
* “MICROSOFT”, “WINDOWS” and “EXCEL” are registered trademarks or Calibration curve data correction, calibration
trademarks of Microsoft Corporation in the United States and other countries. curve tracing
Cumulative data averaging
labeled model is available Statistic calculation
Automatic sensitivity measurement function
Pre-scan
Others
Data transport and graph copying to Microsoft Excel
Print preview function
FL Intensity Standardization
File conversion of 3D Scan Data
NOTES 1. A PC set is not supplied as standard equipment.
Is should be prepared separately.

Science Ring
This logo symbolizes Scientific and Analytical instruments of Hitachi High-Tech Group. It is composed with an “S”, standing for
“Science”, our technology core competency, and with a ring that represents close connection we make with our customers.
This “Science Ring” shows how we are committed to create new values by strengthening ties between Science and Society.

The above logo is a registered trademark of Hitachi High-Technologies Corporation in the United States and other countries.

CAUTION: For correct operation, follow the instruction manual when using the instrument.
Specifications in this catalog are subject to change with or without notice, as Hitachi High-Tech Science Corporation continues to develop the latest
technologies and products for its customers.
NOTICE: The system is For Research Use Only, and is not intended for any animal or human therapeutic or diagnostic use.
These data are an example of measurement; the individual values cannot be guaranteed.

●Sales ●Manufacture

www.hitachi-hightech.com/global/science/ www.hitachi-hightech.com/global/hhs/
Head Office Head Office
24-14, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo 105-8717, Japan 24-14, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo 105-0003, Japan

Printed in Japan (H) HTB-E119 2016.1