Con. 6081-13. , BB-9559 (3 Hours) [Total Marks : 75 N.B.: (1) All quest ins are compulsory (2) Figures tthe right indicate Fol marks. (3) Answer a! sub questions together and in order. (4) Draw nea! labelled diagrams wherever necessary 1.(a) Name the foilov ing (any six) 6 (i) One mu ticomponent analysis technique where absorbance is measured at Amax 0! one compound and at isobestic point One nev et sampling technique in FTIR. Any 2 units for chemical shift value in 'UNMR Spectroscopy. (iv) Any one ionisation technique used in Mass Spectrometry (v) Two supercritical fluids used in chromatography. (vi) A gas chromatographic technique used to quantify residual solvents. (vii) ‘Two chi al detectors used commonly in IPLC plain the full wing terms (any four} ® (i) COSY-SMR \ (ii), MALDI Gil) UPLC (iv) TMA CT ermo Mechan (v) , Chiral Chromatography. (0) Anewer the following (any three) — . (i) Describe use of chemical shift reagent in ‘HNMR spectroscopy with a example (ii) Explain Setro Diel’s Alder rearrangemer t mass fragmentation pathway with a suitabl example. (itl) Give cheracteristic 1, KR. bands observed for Aniline. Cv) Differenvate between taiting factor and asymmetry factor, 2.4a) Answer the falls ving (any two) 8 (3) Give the principle invoived in ion pair chromatography and exp applicati-n of the same in detail. (ii) Explain he principle and give pharmaceutical applications for confocal microsec Gii) Explain sulticomponent analysis of drugs by UV difference speciroscopy. none (b) What is gradient lution? Give its advantages TURN OVERcary ™MaAMy crrsacom weceNteit® t- Con. 6081-BB-95 39-13. 2 : . 34a) Answer the following (any two) (i) Enlist arious interfaces used in LCMS and discuss one of them in detai! (ii) Give te working of attenuated reflectance in FTIR (ii) Explai the sample preparation for the microscopic analysis usi Electre 1 microscopy and give one pharmaceutical application for SEM \ (h) Define coupt ve ¢ stant 4 Z use in structural elucidation 4. (a) Answer the following (any two) (1) Explain the instrumentation of DSC. (ii) Explair finger print analysis using HPLC with a suitable example Gi) Explain with schematic diagram coup ing of a proton with two and three j neighbe aring protons. Show multiplicities with ratios of peaks in the multiplets in both vases. a. (b) Give the form alae for calcul: (2) Resolut/on (ii) Capacit factor § (a) Answer the fo lowing (any two) — (i) Enlist the chiral stationary phases with a suitable example. Discuss a pharma cunieal application using one etiral stationary phase ' Gi vite on FENMR. (iii) Deseribe the instrumentation of Time of Flight analyzer, Give its limitations | (b) With respect tc super critical fluid define critical temperature and critical pressure. ¥ Give any two. nportant properties of super critical Nuid for chromatography (a) Answer the fol owing (any (wo) = 6 (i) Discuss 9 brief MALDI-TLC technique Gi) Give in ¢ tail any three pharmaceutical z pplic Gii) Discuss Vig relationship between column effic Deemter -quation. fons of FGA. ney and flow rate using Van (b) Give the C = 0 treteh IR frequencies for the following : 3 4) oft uns. (ii) Aliphatic xetone. Gili) Aliphatic esters (ix) Aliphatic acid chlorides (9) Anhydrid uated ketone.