Material （

Calibration（ ）・Value assignment（

（ ） Procedure

International conventional
reference measurement
procedure

ICSH recommended
reference method *1

Manufacturer's working Sysmex

calibrator
Fresh human whole blood Manufacturer's standing
Traceability

Sysmex's in-house standard

Manufacturer's product

Sysmex XN CAL

End-user's routine measurement

Sysmex's Hematology Analyzer

Routine sample XN series
EDTA-anticoaglated blood End user

Result

WBC, RBC

Traceability chain of WBC and RBC count

*1: ICSH Expert Panel on Cytometry, Clinical Laboratory Haematology, 16, 131-138, 1994
“Reference method for the enumeration of erythrocytes and leucocytes”
(ICSH: International Council for Standardization in Haematology)

All Right Reserved, Copyright (c) Sysmex 2011

 Material （
Calibration（ ）・Value assignment（
（ ） Procedure

International
conventional reference
measurement procedure

ICSH recommended
reference method *2

Manufacturer's working Sysmex

calibrator
Fresh human whole blood Manufacturer's standing
measurement procedure
Traceability

Sysmex's in-house standard

Manufacturer's product
calibrator
Sysmex XN CAL, XN CAL PF

End-user's routine measurement

procedure
Sysmex's Hematology Analyzer
Routine sample XN series

EDTA-anticoaglated blood
End user
Result

Platelet

Traceability chain of Platelet count

*2: ICSH Expert Panel on Cytometry and International Society of Laboratory Hematology Task Force on Platelet Counting,
American Journal of Clinical Pathology, 115, 460-464, 2001
“Platelet Counting by the RBC/Platelet Ratio method – A reference method”
　　(ICSH: International Council for Standardization in Haematology)
All Right Reserved, Copyright (c) Sysmex 2011
 Material （
Calibration（ ）・Value assignment（
（ ） Procedure
International conventional
reference measurement
procedure
ICSH recommended reference
method *3
CLSI recommended reference
method *4

Manufacturer's working Sysmex

calibrator
Fresh human whole blood Manufacturer's standing

Sysmex's in-house standard

Traceability

Manufacturer's product
calibrator
Sysmex XN CAL

End-user's routine measurement

procedure
Routine sample Sysmex's Hematology Analyzer
XN series
EDTA-anticoaglated blood End user

Result

Hemoglobin concentration

Traceability chain of Hemoglobin concentration

*3: Journal of Clinical Pathology, 49, 271-274, 1996
“Recommendation for reference method for haemoglobinometry in human blood (ICSH standard 1995) and
specification for international haemiglobincyanide reference preparation (4th ed.)”
*4: CLSI H15-A3; Reference and Selected Procedures for the Quantitative Determination of Hemoglobin in Blood – 3rd edition;
Approved Standard

All Right Reserved, Copyright (c) Sysmex 2011

 Material （
Calibration（ ）・Value assignment（
（ ） Procedure

International conventional reference

measurement procedure
ICSH recommended reference
method *5
CLSI recommended reference
method *6 Sysmex

Manufacturer's working
calibrator
Fresh human whole blood Manufacturer's standing

Sysmex's in-house standard

Manufacturer's product
Traceability

calibrator
Sysmex XN CAL

End-user's routine measurement

procedure
Sysmex's Hematology Analyzer End user
Routine sample
XN series
EDTA-anticoaglated blood

Result

Hematocrit

Traceability chain of Hematocrit

*5: Laboratory Hematology, 7, 148-170, 2001

"Recommendations for reference method for the packed cell volume (ICSH Standard 2001)"
*6: CLSI H7-A3; Procedure for Determining Packed Cell Volume by the Microhematocrit Method – 3rd edition ; Approved Standard

All Right Reserved, Copyright (c) Sysmex 2011

 Material （
Calibration（ ）・Value assignment（
（ ） Procedure

International conventional reference

measurement procedure
ICSH recommended reference
method (visual observation method)
Sysmex

Manufacturer's working
calibrator
Fresh human whole blood Manufacturer's standing

Sysmex's in-house standard

Traceability

Manufacturer's product
calibrator
Sysmex XN CAL

End-user's routine measurement

procedure
Routine sample Sysmex's Hematology Analyzer End user
XN series
EDTA-anticoaglated blood

Result

Reticulocyte ratio

Traceability chain of Reticulocyte ratio

*7: CLSI H44-A2; Methods for Reticulocyte Counting (Automated Blood Cell Counters, Flow Cytometry, and Supravital Dyes);
Approved Guideline—Second Edition

All Right Reserved, Copyright (c) Sysmex 2011