A.

IDENTIFICATION/ENUMERATION

1. Who suggested that disease is made by invisible living creatures?

2-3. Who is the father of protozoology and bacteriology ? What did he call the microorganisms he observed ?

4-6. Father of modern microbiology ; what are his attenuated vaccines

7-8. Who developed an antiseptic system in surgery ? ; what is this antiseptic called

9-10. Enumerate 2 medically important bacteria that Robert Koch discovered

11. The use of agar is discovered by

12. The use of petri dish is discovered by

13-16 Who discovered salvarsan ? ; What disease does it treat; What is the bacteria responsible for such disease ;
What special stain is used to stain this bacteria ?

17. What is the genus of the fungi where penicillin is derived from ?

18-19. Taxonomy involves characterization, nomenclature and identification of organisms according to

20. The grouping of classification in taxonomy is?

21. Father of taxonomy ?

22. Discovered the kingdom system based on nutritional pattern

23. Discovered the domain system based on cell function

24-26. Enumerate the 3 classifications of subspecies and explain each

27. Give the family name of Staphylococcus aureus

28-29. Reference culture collections

30-32. Embedded proteins in the cell membrane

33. Point of attachment of nucleoid

34-36. 3 types of cell wall

37. Main component of cell wall

38-39. The answer to # 37 is primarily made of alternating ?

40-41. M protein ( a virulence factor) is primarily found in what bacteria ? What is the purpose of this protein ?

42. A waxy substance that prevents digestion found in acid fast staining cells

43. Responsible for staining characteristics of the cell

44-46. Bacteria with LOS instead of LPS

47-49. LOS has presence of principal proteins / major outer membrane proteins such as

50-51. What are the 2 bacteria that fall under acid fast?

52. Which of these are weakly acid fast

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53-54. The cell membrane of acid fast microorganisms possesses:

55. Glycocalyx that is firmly attached to cell wall

56-59. Polysaccharide containing (55.) ?

60 Polypeptide D- glutamic acid containing (55.) ?

61. Hyaluronic acid containing (55.)

62. physical characteristic of (55.) in an agar

63. Glycocalyx that is unorganized and not firmly attatched to the cell wall

64-65. Polysaccharide containing (63.)

66. Virulence factor for locomotion mostly seen in gram –

67-69. parts of (66)

70. A Sheathed (66) belongs to what genus of bacteria ?

71-75. Classifications of (66.) and describe each

76-78. Stains to view (66.)

79. What is the main component of one of the stains in # 76-78

80-81. Methods to view (66.)

82. Other name for flagella in spirochetes

83-84. 2 types of pili ; give their function

85-88. Gram negative pili containing bacteria

89-91. Gram positive pili containing bacteria

92. Amorphous gel containing enzymes, granules,inclusions and genome

93. Produced by bacteria in harsh conditions; these are highly resistant, dormant and asexual

94. Composition of (93)

95. Staining method for (93)

95-96. The stains employed for (95)

97-98. Give the 2 bacteria that form (93)

99. aka jumping genes ; state the function

100-102. 3 types of main inclusions

103. Glycogen is an inclusion seen in

104-105. Starch is an inclusion seen in

106-107. Poly B-hydroxybutyrate is an inclusion seen in

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108-109. Bipolar bodies are found in these 2 bacteria

110. Sulfide containing bacteria

111-112.Classes of dyes

113-117. Staining procedures ( give 1 example for each )

118. Fluorescent dye for Mycobacterium tuberculosis

119. What method is employed for (118)

120. Gram staining is developed by

121. Accentuator in acid fast staining?

B. TABLE COMPLETION

Prokaryotic Eukaryotic
Nuclear body

Genome

Cell division

Cell wall ( also include exceptions)

Site of electron transport chain

Ribosomes
Cytoplasmic membrane (also
include exceptions)
Motility

Presence of organelles

Gram + Gram -
Murein layer
Cell wall

Periplasm
Outer cell membrane

S-layer

O-antigen K-antigen Vi-antigen H-antigen

Heat labile / stable
Found in :

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Inclusion bodies Bacteria Types of inclusions Stain to employed for viewing
(if specified otherwise choose
between gram staining and acid
fast )
Much granules

Iodine

Basophilic

Corynebacterium
diphtheriae

Special stain (give at least 1 ) Part being targeted by the stain

Cell wall

Spirochete

Feulgen

Albert

Endospores
Flagella

Reagents Gram + Gram -

Primary stain

Mordant
Decolorizer

Counter stain

Reagents Hot method / Cold method/ Acid fast Non-acid fast

Primary stain

Mordant

Decolorizer
Counter stain

JTC2019