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Lab 1 : Viral Isolation - Direct Detection

of Virus in Clinical Specimen


Lab a parts
diag
1. isolation I culture Tonita otosrik35W niwmrntnwwiwngrn
pock formation hem agglutination test

haem
egg
:
,

culture :
cytopathic effect plaque
,
formation , adsorption
2.
Ag detection
immuno

Emmivirion
method

immuno
non : ,
staining m inclusion

method : It
,
IP
,
ELISA ,
WB

direct latex
detecting :
agglutination immunochromatography ,

3 .
Ab detection

function : hem agglutinin inhibition neutralization complement fixation test


, ,

non -
function : ELISA WB

of plaque in plate

molecular
diag
21 .
GOLD STD .

!1!

A IAOMFM
mom On site non
a

1 onion virus
Ygfwuwton influenza
'

9Fur7wgw
9wmrTn
2
purpose
ni vaccine
f @ diagnosis
e. dx
g. ,

@ in vaccine


oporouwon
gtomwn Minn vaccine %NTnMroW%Wni%U3mmmnni7
ioiwrliwni hem agglutinin test IN
CEP

CEHTIIIWW
inclusion body
1. indoor eg
multinucleated giant cell
2. NOWMJIMW
syncytial formation
RYTMFJOOITCPE birinivirusoioacpenignow celllysis

muscle all

(
spindle shape) voo virus noiniownmioriw

p %%0Mh0nYn% ' cell IT minor virus


Tin ngowtnrjnotonw -

TJJMOJI lowdown

gold standard
culture iidiqdiauoow'D virus on cPEu% plaque Not

↳ 9 iiwcelwon

g. w}nmtrnwTrToowj rroinrsiiarovirusoignroon
irntoioiwrkrnoioohwqn report
Nws
direct immuo

!2!

I .

2 . ( cnz .

assay )
!3!

of
1 . WN
neg

pos
at pos resolution )
(
neg
→ .

ioironorouwn's
2.
{ } going to
e.

nrntstskioi

Milito
3 .

NOIGM

boiinotsironmirrtoinwotooq
n'T clinical now
OTNFEOISJWWJN tilculture
Monty
: ,

2Wh
'

MHo1gM
;
" "

strip

AY fix Ab test control


INNYAG ,Ab

sandwich
Mining
memo

DRQP
SNQW
South West PNA protein

north RNA

Mwh
40
9 Fprobefispecificriovirus
e. virus TTG
g.
probe AAC
CPE
=qnwwr8io9o


cpeoiosgcelt

:
culture lisolation

.PE

✓ Tscperdwmoqcell

active Implication
ndusionbodyrdwlswc
× .

✓ gfnron isolation mints


nrirnwinfectnai negative
iroioiosrssomoowjua.mn#omstWva8

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