Академический Документы
Профессиональный Документы
Культура Документы
(ICPEP-6)
27-30 November, 2018
Organised by
International Society of Environmental Botanists
&
CSIR-National Botanical Research Institute
Lucknow, INDIA
Abstracts
(ICPEP-6)
27-30 November, 2018
Organised by
International Society of Environmental Botanists
&
CSIR-National Botanical Research Institute
Lucknow, INDIA
Publication Committee
Dr. P.A. Shirke
(Chairman)
Organizing Secretaries
Dr. R.D. Tripathi Dr. Nandita Singh Dr. Vivek Pandey
Preface
E nvironmental pollution is increasing exponentially with each passing season due to drastically
changing human needs. The "Sixth International Conference on Plants and Environmental
Pollution" (ICPEP-6), to be held in Lucknow, India, would be the venue when the luminaries of
Environmental Science across the world would assemble to discuss and deliberate on the pressing
environmental issues, and lay the path for future course of actions and research in the area of Plants and
Environmental Pollution.
The "Sixth International Conference on Plants and Environmental Pollution" (ICPEP-6), was able
to kindle for submission of about 413 abstracts for the four day event. These abstracts have been
compiled into this "Abstract Book" which we received from the distinguished in the area of
Environmental science to the novice research scholars, however, with gleam of new ideas.
The abstracts included in this volume were received within the prescribed time limit. All the
submissions were peer reviewed and edited and classified into the eight themes of the conference and
the abstracts were segregated in different sessions for oral and poster presentation. This time there was a
lot of zeal from the young researchers for the oral presentation, keeping their enthusiasm in mind, the
ratio of oral presentations were increased as compared to the last conference with opportunities to the
young minds as well as to the peers of the respective sessions.
We place on record our deep sense of appreciation to Prof. S.K. Barik, Director CSIR-NBRI and
President ISEB, for his whole hearted support in terms of logistic and expert opinion. We are thankful to
Drs. R.S. Tripathi, P.V. Sane and K.J. Ahmad for their timely apt suggestions and encouragements. We
are beholden to our contributors who regardless of their various assignments and commitments
acceded to our request to commit their valuable time and expertise.
We are thankful to the Scientific Program Committee for categorizing the abstracts and editing
them. We are also thankful to the staff of ISEB Secretariat for their co-operation in various ways.
We are indebted to the various sponsoring agencies for financial support which made this
publication accomplishable. Finally the efforts of M/S Army Printing Press and their team
metamorphosed the publication to the present form, we sincerely thank these people.
Publication Committee
ICPEP-6
Scientic Programme Committee
Prof. R.S. Tripathi
(Chairman)
Session S-V : Sub-tropical and Tropical Oak under Global Change 113
Special Lectures
scale -local, regional, or global. Biodiversity has evolved under cared and often taken for granted. Wide spread
over millennia, is inherently dynamic, multidimensional, deforestation, loss of habitat, growing pollution and
omnipresent and, regarded to have transformed earth’s climate change are serious threats to biodiversity. The
primeval atmosphere into oxygen rich atmosphere and current rate of global diversity loss is estimated to be
more importantly it is only system that harvest and 100 to 1000 times higher over the background extinction
stores solar energy at a planetary scale. Humankind is rate. It is estimated that half of all the species on the
obligately dependent on biodiversity for food, breath, planet could go extinct by 2050- only 32 years from
medicine and other basic needs and wellbeing. The now. Biodiversity loss is a crisis of historical and
nexus between biodiversity and human civilization is planetary proportions. Biodiversity loss may amplify
the single most important factor responsible for climate warming, and could lead to additional,
sustaining human survival, scientific and industrial unforeseen, and potentially irreversible shifts in the
progress, economic growth and cultural evolution. earth system. Despite numerous commitments,
Biodiversity viewed in the context of sustainable biodiversity loss continues to accelerate in all
development offers opportunities for poverty regions.The situation is alarming and unless urgent
eradication, human well-being and livelihood. In spite action taken now, poverty eradication, food and water
rapid stride in the growth of science and technology security and sustainable future will remain elusive.
human dependence on biodiversity is rising and likely Key words: Biodiversity, Pollution, Climate change,
to remain indispensable in foreseeable future. Extinction.
Unfortunately, biodiversity is grossly undervalued and
Session I
Climate Change: Adaptation, Mitigation,
Carbon Sequestration and Food Security
resilience of urban trees and forests to multiple stressors One-year (2014-2015) observations on the
of climate change and pollution. We will demonstrate ecosystem scale CO2/H2O fluxes and other micro-
how we could combine basic research, applied meteorological observations were analysed for
research, policy research, governance, and civil society elucidating the diurnal and seasonal pattern of carbon
under a trans disciplinary framework. We will present fluxes together with their response to biophysical and
a case of on-going research in the city of Karlsruhe, environmental parameters. Daily average minimum and
Germany which is funded by the “Future Cities” maximum air temperatures in the study area ranged
program of the German Government. Our presentation between 10.31 to 32.36 °C. Environmental factors
may motivate Indian researchers, city planners, and governing carbon flux components were found to be
policy makers to formulate similar trans disciplinary related to maximum and minimum net ecosystem
research for urban trees and forests in India cities; exchange (NEE) of carbon, GPP, and the ecosystem
where problems of urban sprawling, pollution, and respiration (Re). On daily scale, maximum absorption
climate change are acute. We believe our presentation of carbon took place on 244th day of the year (DoY)
fulfills: the objective of the “Smart Cities” program by with a NEE value of -5.51 gC m-2 day-1 while maximum
the Indian Government, and Indo-German bilateral release took place on 219thDoY with a NEE of +7.83
research focus on climate change adaptation for future gC m-2 day-1. Highest GPP was observed on 244thDoY
cities. with a value of 14.12 gC m-2day-1 while lowest of 0.21
Key words: Climate change, Air pollution, Vulnerability gC m-2 day-1 on 61thDoY.Highest Re of 11.94 gC m-
2
assessment, Urban forestry, Urban greening, Environmental day-1 was noticed on 218thDoY and lowest of 2.50 gC
governance, Adaptation planning m-2day-1 on 12thDoY. Clear variations in monthly NEE,
GPP and Re were observed during the study period.
SII/L-4 Higher leaf area index (LAI) and sufficient moisture
conditions resulted in higher carbon sequestration during
Climate Change and Carbon September through March. During pre-monsoon season
Sequestration Monitoring in India (April-June), the NEE was lower due to lower LAI
and higher vapour pressure deficit (VPD). During the
S.P.S. Kushwaha rainy season (July-August), the carbon sequestration
potential was highly impeded by cloudy conditions and
Indian Council of Forestry Research and Education,
the rain-accelerated Re. Annual GPP, Re, and NEE of
Dehradun-248006, Uttarakhand, INDIA, Email:
spskushwaha@gmail.com sal forest was 2916.19 gC m-2year-1, 2408.32 gC m-
2
,and -507.89 gC m-2year-1.
Forests play a vital role in maintaining the
atmospheric carbon balance by sequestering it into For upscaling of GPP to entire Dun Valley biome,
products like biomass, wood, and timber in addition to four remote sensing-driven models, viz., (i)
improving the soil fertility, providing a large number of temperature-greenness (TG) model, (ii) greenness-
ecosystem services, and thus, mitigating climate change radiation (GR) model, (iii) light use efficiency (LUE)
over time. However, lack of accurate and spatially- model, and (iv) satellite image-based LUE (LUERS)
explicit information on the carbon exchange components model were parameterized with EC measurements and
from diverse forest ecosystem types in India limits compared with 8-day Moderate Resolution Imaging
regional scale carbon budgeting. Remote sensing-driven Spectroradiometer (MODIS) GPP products. Our
ecosystem models are well-established tools for results showed that TG model had the highest root
estimating gross primary productivity (GPP) over large mean square error (RMSE) of 1.28 gC m-2 day-1 while
areas. The present study usedtall tower eddy covariance GR and LUERS models had moderate RMSE of 0.99
(EC) data and satellite-derived variables to estimate gC m-2 day-1and 0.98 gC m-2 day-1, respectively. The
the carbon source/sink status of the a northern Indian closest GPP estimate was obtained through LUE model
mature moist deciduous/semi-evergreen sal (Shorea with RMSE of 0.93 gC m-2 day-1.The RMSE for all
robusta) forest at Barkot in Dun Valley and upscale four models were 4 times lower than that of MODIS
the GPP over entire Valley sal forest area. GPP. Lower maximum LUE ( in MODIS GPP
algorithm contributed to higher RMSE. Our study be termed as nitropriming for its enormous beneficial
highlights the need of EC data for estimation of net effects instead of nutripriming. During nitropriming
ecosystem level carbon exchange and calibration of NO3 ion in fluxed in the seed and NO, an important
satellite image-based GPP model for upscaling over signaling molecule forvarious abiotic stresses may be
large area. generated via the increased activity of nitrate reductase
Key words: Climate change, Carbon sequestration, GPP. (NR) enzyme, as was observed in nitro primed okra
seeds. Therefore it can be concluded that nitropriming
SI/L-5 is a suitable option to adopt for Climate Resilient
Agriculture in this era. This lecture will summarize the
Nitrate Seed Priming (Nitropriming) impact of nitropriming on important field crops and its
possible mechanism of action in respect to stress
vis-a-vis Climate Resilient Agriculture amelioration in various field crops.
Bandana Bose Key words: Climate change, Seed, Nitro priming, Stress
responses, Field crops.
Seed Priming Laboratory, Department of Plant Physiology,
Institute of Agricultural Sciences, Banaras Hindu University,
SI/O-1
Varanasi-221005, INDIA, Email: bbosebhu@gmail.com
In this century we are facing the biggest threat Adaptive Capacity of Different Norway
of climate change due to increasing population, demand Spruce Provenances to Lethal Drought
for food, fodder and shelter, industrialization,
urbanization including squeezing and over exploitation Tamalika Chakraborty*, Tomasz Czajkowski and
of arable land. Climate is now changing very erratically. Andreas Bolte
Hence it is the time for Climate Resilient Agriculture
so that our planet can be ready to face the challenges Thünen Institute of Forest Ecosystems, Alfred-Möller-Str.
1, 16225 Eberswalde, Germany Email: tamalika.chakraborty@
of food security now as well as in future by maintaining
thuenen.de, tomasz.czajkowski@thuenen.de, andreas.bolte
the sustainable development via adopting various @thuenen.de
technologies. Seed, being best delivery system in
agriculture the plant scientists are engaged in Events of severe heat waves and droughts are
developing various technologies which can protect crop becoming frequent in central Europe and impacting
seeds from various stresses, faced during germination forest ecosystems. Norway spruce (Picea abies) is
in field. Seed priming is where controlled hydration and the most important commercial tree species in Germany.
dehydration is employed in seed by initiating the process However, spruce is sensitive to drought, and thus
of germination but the protrusion of radicle is restricted. maintaining forest structures with spruce will increase
Seed priming ensures proper and synchronized the risk of forest damage and loss of economic value.
germination and seedling development. Since more than Our recent studies on European beech (Fagus
three decades Bose and her group are working with sylvatica) revealed a high variation of drought
seed priming technology using various salts containing tolerance among beech provenances. This enables to
nitrates, phosphates, carbonates and sulphates and have increase drought resistance and resilience of beech
observed that among these, salts of nitrates performed forest stands by selecting more drought tolerant
best in normal as well as in stressed conditions. Plants, provenances from the rear edge of the beech forest
obtained from nitrate primed seeds develop the capacity distribution with low precipitation rates within the
to tolerate the drought, heat, cold, submergence and growing season. Motivated by our previous research
heavy metal stresses. This treatment also improves the on European beech, we studied response of Norway
quality of the produce. The influence of nitrate spruce sourced from different European provenances
treatment has been drawn on the basis of studies related to extreme drought. In a greenhouse pot experiment, a
to germination physiology, nitrogen and antioxidant lethal drought was simulated using spruce seedlings of
metabolism in the growing plants and their yield eight provenances in summer of 2017 (treatment: 100
attributes. Therefore now this treatment technology can plants per provenance, control: 20 plants per
provenance). Drought was applied until plants’ death; because the country is endowed with a variety of plants
during the experiment soil water availability (SWA, and animal species found in the boundless forests and
percentage proportion of available water AW), gas grasslands. Nigerian plant species occur in different
exchange, plant water status (pre-dawn water potential) numbers within the country’s vegetation that range from
and survival rate for each provenance were recorded. the rainforest and mangrove along the coast in the South
Based on survival rates, symmetric logistic survival to the Sahel in the North. Most of the plant species
functions (2 PL) for the different provenances were sustain the rural economy. Attention of Nigerians has
fitted. We found varying responses of mortality of been focused on plant species because of their forestry
plants between provenances to drought. The critical importance and commercial uses in a number of ways
mortality rate of 50% (LD50) at remaining soil water that affect human lives such as construction works,
availability (SWA) ranged from 7% for a provenance nutrition, entertainment, forensic medicine,
from East-German lowland with low yearly precipitation pharmaceutical and cosmetic industries. In spite of the
to 8.3% for a Bavarian mountainous provenance with socio-economic and medicinal values of the plant
considerably higher yearly precipitation. First seedlings species to human well-being and sustainable
of all provenances died when SWA had fallen below a development, Nigerian plant species are still facing
critical threshold of 20%. These outcomes demonstrate prejudicial challenges of climate change. These
the general possibility to increase drought resistance ecological dilemmas were directly attributed to high
of Norway spruce regeneration by using more drought- level of human activities due to deforestation, over
tolerant provenances. population, over harvesting of wild plants for logging,
Key words: Adaptive forest management, Climate change medicine and food; pollution in the cause of energy
impacts, Drought, LD50, Provenance trial, Tree mortality. generation through firewood, electric power supply and
industrialization processes. The consequent effects of
SI/O-2 which were decline in soil fertility, lowering of potable
water, shift in the seasons, starvation, increase in
Potential Impacts of Climate Change incidence of pests and diseases and increased crime
and Air Pollution on Plant Diversity in rate. This paper highlights the threats, challenges and
effects of climate change and air pollution on the
Nigeria: A Review Nigerian plant species as well as suggesting possible
remedy to the menace through intensive afforestation
O.M. Mudasiru1,2*, A.E. Ayodele2 and T.S. Rana1 programmes by establishing woodlots, shelterbelts,
1
Plant Diversity, Systematics & Herbarium Division, CSIR- orchards and agro-silvicultural system as well as the
National Botanical Research Institute, Rana Pratap Marg, application of effective environmental management
Lucknow-226001, Uttar Pradesh, INDIA; 2Department of actions to reverse the process and safe-guard future
Botany, University of Ibadan, NIGERIA Emails: occurrences.
opeyemi.mudasiru2013@gmail.com, bayodele@yahoo.com,
ranats@nbri.res.in, a.e.ayodele@mail.ui.edu.ng, ranatikam Key words: Climate change, Deforestation, Air pollution,
@gmail.com Over-harvesting, Nigeria.
unfavorable condition. Plants selected for this study mismanagement of apple and cherry, and postharvest
were Cynodon dactylon, Nerium oleander and Ficus sprouting of onions and potatoes. Following standard
benjamina. Leaf samples collected from the plants procedures of harvesting and post harvest handling can
were studied and analysed. Physical characters were drastically reduce postharvest losses, with no or little
includes leaf area index, dust load on the leaves, injured additional cost, but certainly high returns. However,
leaf area, pH of soil and water holding capacity of soil. stakeholders, especially growers, neither know the
Physiological characters were includes protein content cause of problem nor the means to control it. So first
of leaves, starch content of leaves and chlorophyll and foremost step would be to sensitize and practically
content of leaves and also total carbon content of soil. educate them about the causes of problem, and the
The results of this study found that the plants growing need and ways to control it. Though simple and standard
in favorable region have more productivity as compared techniques do exist in developed counties to address
to the ones growing in unfavorable region. Plants many such causes, but the access/means and
growing in garden site differ from plants growing in knowledge about them is lacking among stakeholders
roadside both morphologically and physiologically. in developing world (like Kashmir). Thus, practical
Key words: Leaf area index, Dust load, Water holding education/ training of stakeholders through Postharvest
capacity, Roadside. Training and Services Centers (PHTSC) can help in
reducing Postharvest Losses, which in turn can increase
SI/O-4 food availability and food security and thereby reduce
over-reliance on production-oriented activities and
Postharvest Training and Services hence help in environmental protection.
Centers can Help in Food Security and Key words: Postharvest Losses, Training, Education,
Knowhow, Handling, Food security, Pollution.
Environmental Protection by Reducing
the Postharvest Losses SI/O-5
acts as natural conservation microrefugia for many countries particularly India. Conservation agriculture
threatened species endemic to Himalayas. The present (CA) offers a low-cost strategy for mitigating climate
study models the potential distribution of J. squamata change by sequestering carbon in soil and is based on
using ensemble approach in Biomod2 package for the principles of minimal tillage, residue recycling and
present and future (RCP’s 2.6 and 8.5 covering 2050 crop diversification for resource conservation and
and 2070). The results indicated an expansion of sustainable agriculture promote soil carbon restoration
climatic suitability of J. squamata towards much higher by tipping the balance in favor of carbon inputs relative
elevations into areas that are currently unsuitable and to carbon outputs. Carbon storage (sequestration) in
on the other hand, several currently suitable areas may the soil can be achieved by maximizing carbon inputs
become climatically low or unsuitable in the future. and minimizing carbon outputs. Soil carbon fractions
Currently, the total climatically suitable area for this are influenced by the agronomic management practices
species is 126774.66 km2 , with an additional area adopted in CA systems. Contrasting cropping systems,
becoming suitable under RCP 2.6 (2050 and 2070) and tillage and residue management have a
RCP 8.5 (2050 and 2070). The results also revealed a significantinfluence on soil quality, C and N cycling.
northward shift and upslope advance of existing suitable SOM stratification (SR) and carbon management index
climate for J. squamata in future climate scenarios. (CMI) as an indicator of soil quality, related to the rate
Hopefully, the research insights from the present study and amount of SOC sequestration generally used for
will help in understanding the impacts of climate change natural and managed ecosystems. In general, the high
on alpine vegetation in Himalayas with wide implications values of stratification ratio (proportion of a soil
for scientifically-informed adaptation and mitigation property at the surface layer to that at a deeper layer)
strategies. indicate good soil quality and are usually used to assess
Key words: Alpine, Climate change, Himalayas, Microrefugia, agricultural practices. Experimental trials in two
Mountains. research station (Karnal, Haryana, India and
Samastipur, Bihar, India) on CA was established in
SI/O-6 2012.This study aimed to 1) assess the stratification of
soil carbon pools; and 2) evaluate the soil quality of
Impact of Conservation Agriculture different CA practices using SR and CMI values as
Practices on Productivity and the primary assessment parameters. Soil carbon stock
increased in zero tillage and permanent beds as
Stratification of Soil Carbon in Indo- compared to conventional practices. Responses of SR
Gangetic Plains of India in different CA practices to change of soil depth were
significantly different with the highest value in zero
Ajay Kumar Mishra1*, Hitoshi Shinjo1, Hanuman tillage. The SR values of labile carbon fraction differed
Sahay Jat2, Mangi Lal Jat2, Raj Kumar Jat3 and significantly only up to plow layer (0-5:5-15 cm). Short-
Shinya Funakawa1 term practices (5-years) of CA in Indo-Gangetic plains
1 of India influences the distribution and stratification of
Terrestrial Ecosystem Management Laboratory, Graduate
School of Global Environmental Studies, Kyoto University, soil carbon pool. Our study revealed that SR of carbon
Kyoto, JAPAN; 2 International Maize and Wheat pool serves as an efficient indicator for short-term
Improvement Centre, New Delhi, INDIA; 3Borlaug Institute changes offered by CA practices. CA systems not only
of South Asia, International Maize and Wheat Improvement sequestering substantial carbon in soil but also enhances
Centre, Bihar, INDIA, Email: akm8cest@gmail.com productivity and resilience of the cropping system to
Climate change and food security threatens the attain the goal of sustainable agriculture.
present agriculture and is of great concern in developing Key words: Carbon sequestration, Stratification ratio,
Infiltration rate, System productivity, Residue load.
SI/O-7 SI/O-8
Ajeet Singh* and P.C. Abhilash Department of Forestry, North Eastern Regional Institute of
Science and Technology (Deemed to be University), Nirjuli-
Institute of Environment & Sustainable Development, 791109, Arunachal Pradesh, INDIA, Email: ritashree.100@
Banaras Hindu University, Varanasi-221005, INDIA, gmail.com
Email: ajeetbhu97943@gmail.com
The study was carried out to determine the carbon
Winged bean (Psophocarpus tetragonolobus stock and net carbon sequestration potential of land
DC), aptly described by National Academy of Sciences, cover under Citrus sinensis plantation in tropical agro-
United States of America as a ‘supermarket on a stalk’ climatic zone of Arunachal Pradesh. Altogether, fifteen
is an underexploited legume of the tropics. The leaves, (0.1ha each) permanent plots were established along
shoots, flowers, young pods, seeds and tubers are all the altitudinal gradients and carbon stock was measured
edible and rich sources of proteins, minerals and using standard methodologie / allometric equations and
vitamins. The crop can be luxuriously grown in hot and carbon sequestration potential using age of the
humid tropical countries and can be easily grown on plantation crops. Soil samples were also collected in
roadside, boundaries of field, and home gardens. Owing replicate and brought to the laboratory for further
to its high nutritional significance and ecological chemical analysis. Stand density of the plantation ranges
adaptations, it can be projected as a futuristic crop from 476 stems/ha to 620 stems/ha having the basal
under changing climatic conditions. Therefore, area variation of 3.36 m2ha-1 to 10.63 m2 ha -1. The
extensive field survey has been conducted in Eastern individuals have been grouped into four age classes
Uttar Pradesh for knowing the occurrence and i.e., 10, 15, 20, >25 years. The aboveground biomass
distribution of winged bean as well for collecting values of the plantation crop were 23.45 Mg ha-1, 30.57
germplasm for large-scale exploitation. While the Mg ha-1, 70.89 Mg ha-1 and 71.43 Mg ha-1 in aforesaid
cultivation of winged bean is extremely low in Eastern age-classes. The belowground biomass was 8.54 Mg
UP, our study indicates that it can grow well in hot and ha-1, 8.86 Mg ha-1, 20.56 Mg ha-1 and 20.71 Mg ha-1 in
humid environment and even in disturbed and nutrient 10, 15, 20 and >25 year old plantation, respectively.
poor soils. Largescale exploitation of winged bean can Total biomass carbon calculated from the plantation
complement the food and nutritional security initiatives crop ranged between 20.89 Mg C ha-1 and 50.67 Mg
in tropical countries and therefore, suitable conservation C ha-1 and biomass carbon accumulation was positively
and crop improvement programs are imperative for correlated and increased with increase in age of
ensuring the environmental sustainability of winged plantation (r2 =0.99). The soil organic carbon content
bean cultivation. ranged from 35.89 Mg C ha-1 to 75.76 Mg C ha-1
Key words: Environmental sustainability, Food security, among plantation the different ages. The litter carbon
Nutritional security, Tropical countries, Winged bean. content varied from 0.56 MgC ha-1 to 2.46 Mg C ha-1.
Total carbon stock in the present study ranges from
57.79 Mg C ha-1 to 101.44 Mg C ha-1. It was found
that older plants sequester comparatively more carbon
than the younger ones except in some of the carbon
pools like litter and soil, which could be mainly attributed
to management practices in form of litter collection.
The combined approach of statistical regression along
with field based data were used to predict the carbon SI/O-10
density map using various geospatial tools coupled with
satellite data. Detection of Mycoflora and Mycotoxin
Key words: Carbon sequestration, Citrus plantation, in Raw and Roasted Peanut Kernels
Regression, Vegetation indices. in Bangladesh
SI/O-9
Md. Maniruzzaman Khandaker 1 *, Tasmilur
Integrated Farming Strategies for Rahman1, Motiur Rahim2, Maksuda Begum2 and
Tariqul Islam2
Climatic Resilient Agriculture under 1
Department of Botany, Jagannath University, Dhaka,
Rain-Fed Conditions in North West BANGLADESH; 2Food Toxicology Research Lab, BCSIR,
Himalayan Regions Dhaka, BANGLADESH, Email: maniruzzamanbot@gmail.com
Raw peanut kernel samples were collected from
Gulshan Kumar* and Hem Chander thirteen areas of Bangladesh for determination of
Division Botany, Department of Bio-Sciences, Career Point mycoflora and mycotoxin. Fungi associated with the
University Hamirpur, Himachal Pradesh-176041, INDIA tested samples throughout the investigation were
Email: sharmagulshan1980@gmail.com, hemchander78@ Aspergillus flavus, A. niger, Aspergillus sp. (1),
gmail.com Aspergillus sp. (2), Penicillium sp., Fusarium sp.,
The results of the national innovation on climatic Rhizopus sp. and Curvularia sp. A. flavus was the
resilient agriculture (NICRA) investigations on most occurred fungus followed by A. niger. Colonies
integrated farming strategies to cope with rain-fed of fungi were formed in 79.81% to 98.10% of raw
conditions of Hamirpur district of Himachal Pradesh peanut kernels. Out of the thirteen samples, eight
are being described. On the basis of hydrological and samples were found to be contaminated with the range
technical as well as social and cultural conditions, 11.91ppb to 182.62ppb of total aflatoxins and five
appropriate technological strategies have been samples were free from aflatoxins. Another ten
implemented to combat the effects of climate change. samples of roasted peanut kernels were collected from
The main objective was to enhance the resilience in different areas of Bangladesh for determination of
agriculture. The top most priority of the present study mycoflora and mycotoxin association. Fungi associated
was to way out the source of water for meeting with the roasted samples were Aspergillus flavus,
minimum critical water needs of crop diversification Aspergillus niger, Aspergillus sp. (1), Aspergillus sp.
and maintenance of ecological balance under rain-fed (2), Penicillium sp., Fusarium sp., Rhizopus sp. and
conditions and to analyze their technical/economical Curvularia sp. A total of 3630 fungal colonies were
feasibility as well as their affordability for future users. formed. Among these fungal colonies the highest
The present study reveals that it is economically feasible 39.12% of colonies were formed by Aspergillus flavus
to apply decentralized technologies and strategies in and the lowest 1.68% colonies were formed by
farming system for climatic resilient agriculture Curvularia sp. The highest 73.91% of the kernels of
universally. sample 01 were found infected while in sample 05 it
was 25.17%. Out of ten roasted peanut samples four
Key words: Crop diversification, Livestock, NICRA, Rain- were detected with various amounts of aflatoxins (B1,
fed, Resilience. B2, G1, G2). The highest 38.94 ppb of total aflatoxins
were detected in sample 02 and the lowest 5.67 ppb of
total aflatoxins were found in sample 01. Six roasted
samples were free from aflatoxins.
Key words: Raw peanut kernel, Roasted peanut kernel,
Mycoflora, Aspergillus flavus, Aflatoxin.
taxa namely Alnus, Corylus, Betula, Carpinus, difference (p<0.05). On an average, the yield of maize,
Quercus suggesting a relatively warmer temperate turmeric and ginger intercropped were 2.68, 6.15 and
climate in the region supporting the vegetation that 6.96 t ha-1 respectively, which are lower than average
grows well in frost free climatic conditions. Similar yields from sole crops. It was observed that the
synchronous trend of high to low percentage of intercropping of maize had significantly (p<0.05)
pteridophytic and bryophytic spores along with fungal increased yield while turmeric and ginger decreased
spores was observed. The study is also supported by over the years. Maximum above ground biomass (33.35
moderate percentage of dinoflagellate cysts, poorly Mg ha-1) and carbon storage (15.67 Mg C ha-1) was
preserved brackish water centric diatoms indicating the found in Oil palm +Maize intercropping systems with
paralic sediment deposition. The inferred warm significantly higher carbon sequestration rate (5.22 Mg
temperate climate in the Arctic polar region during the C ha-1 yr-1) as compared to other treatments. The results
mid-Pliocene and its consequences on the Asian indicate that intercropping in juvenile oil palm plantations
Monsoon system is potentially important for predicting could have coupling effects both beneficial in providing
the impacts of global warming in the near future. additional crop yield and also carbon sequestration
Key words: Pliocene, Climate, Vegetation, Arctic, India. services. The practice can be an alternative to shifting
cultivation and helpful in reclaiming degraded lands in
SI/O-13 the wake of climate change mitigation efforts.
Key words: Intercropping, Oil palm, Carbon sequestration.
Intercropping Practices in Juvenile Oil
Palm Plantations to Evaluate Crop SI/O-14
Yield and Carbon Sequestration Prospects of Bamboo Resources
Services in Mizoram, Northeast India Available in Northeast India in Boosting
Soibam Lanabir Singh, Uttam Kumar Sahoo* and Socio-Economic Condition of the
Anudip Gogoi People and in Mitigating Climate
Department of Forestry, School of Earth Sciences and Natural Change
Resource Management, Mizoram University, Aizawl-796004,
Mizoram, INDIA, Email: uksahoo_2003@rediffmail.com; L.B. Singha1*, O.P. Tripathi1, M.L. Khan2 and R.S.
uttams64@gmail.com Tripathi3
Oil palm cultivation involves a long gestation period 1
Department of Forestry, North Eastern Regional Institute
(3-4 years) in the initial years after planting posing a of Science and Technology (Deemed to be University),
threat to smallholder farmers, and hence intercropping Nirjuli, Arunachal Pradesh, INDIA; 2Department of Botany,
could provide for additional revenue. The experiment Dr. H.S. Gaur Central University, Sagar, Madhya Pradesh,
was carried out between 2016 and 2018 to determine INDIA; 3National Botanical Research Institute, Lucknow-
the yield of crops and its effect on growth and 226001, UP, INDIA Email: lbsingha@hotmail.com,
development of main oil palm. Different intercropping khanml61@gmail.com, tripathirs@yahoo.co.uk
experiments were designed with standard oil palm Northeast India is rich in bamboo resources as it
density (148 palms ha -1 ) in an intensive three-year provides favorable ecological niches for different
sequential cropping. Complete randomized block design bamboo species. The region harbors more than 60%
with three replicates was adopted and the treatments of the country’s bamboo resource in terms of diversity,
consisted of: Sole oil palm (T0); Oil palm + Maize (T1); and more than 66% of the growing stock of bamboos
Oil palm + Turmeric (T2); and Oil palm +Ginger (T4). is present in this region. They are mostly woody, native
Results showed that maximum oil palm growth and endemic. Only a few exotic species are found in
development in terms of height, girth and estimated the region. Bambusa balcooa, B. tulda, B. nutans,
above ground biomass was observed in T0, however, B. cacharensis, B. polymorpha, Dendrocalamus
the different treatments did not have any significant giganteus, D. hookeri, D. sikkimensis, D. latiflorus,
Schizostachyum dullooa, Melocanna baccifera etc. the main determinant of the ecosystem C-balance.
are large-sized bamboos with high commercial values Changes in soil organic carbon storage as well as in
and multiple applications. Tender shoots ofca.16 soil respiration may affect the atmospheric CO 2
bamboo species in both fresh and fermented forms are concentration and global carbon cycle. Thus the present
highly commercial as vegetable with an annual study was undertaken to find the affects of
consumption of more than 7000 tonnes and a net annual environmental parameters on soil respiration rates
income of ca. 50 million rupees, contributing under Pinus roxburghii vegetation cover using
significantly to the socio-economic condition of the rural environmental gas monitor instrument. CO2 emissions,
people. Most of the bamboo species occurring in the soil temperature and soil moisture were recorded on
region are grown in home gardens, whereas few monthly basis. Results of the study reveal that the rate
develop into pure brakes forming secondary of soil respiration increased with increase in
successional forests. Due to fast growing nature, temperature and soil moisture. Thus, the soil
bamboos act as an important carbon sink. The rate of temperature and soil moisture content exerted the
carbon sequestration by bamboos may exceed that of primary and secondary factors to control soil respiration
the native tree species. Large-sized bamboo species respectively. Maximum soil respiration rates were
accumulate more than 290 tonnesha-1 biomass with a observed during June (0.34 g CO2 m-2 hr-1) and July
net primary productivity of ca. 47 tonnes ha-1 yr-1, which (0.75 g CO2 m-2 hr-1). These rates may be attributed to
is almost double the rate exhibited by eucalypt clones. the high soil temperature and soil moisture rates during
The carbon pool in standing crop of many bamboo these months besides higher air temperature and
species with ca. 149 tonnes of C ha-1 in 6 years old precipitation factors which start declining from August.
plantations is even higher than that of 40 years old teak Seasonally, soil respiration showed positive relationship
forests which accumulate hardly ca. 126 tonnes of C with soil temperature and soil moisture. Therefore, soil
ha -1 . respiration rates can be affected by climate change.
Key words: Bamboo resources, Northeast India, Carbon Key words: Soil respiration (Rs), Soil Temperature (Ts), Soil
sequestration, Carbon pool, Standing crop Moisture (SM).
SI/O-15 SI/O-16
Soil Temperature and Soil Moisture Ecosystem Carbon Pool and Net
Influence on Soil Respiration Rates Primary Production in a Tropical
under Pinus roxburghii Vegetation Matured Forest of North-Eastern
Cover India
S. Sivaranjani* and V.P. Panwar Ratul Baishya1* and Saroj Kanta Barik2
1
Forest Soils Discipline, Forest Ecology and Climate Change Department of Botany, Faculty of Science, University of
Division, FRI, Dehradun, INDIA Email: ranjani.agri@gmail. Delhi, Delhi-110007, INDIA; 2National Botanical Research
com, vppanwar@yahoo.com Institute, Rana Pratap Marg, Lucknow-226001, INDIA, Email:
rbaishyadu@gmail.com, sarojkbarik@gmail.com
The amount of soil respiration that occurs in an
ecosystem is controlled by several factors. Carbon Tropical forests of north-east India provide such
enters in terrestrial ecosystem through photosynthesis vital ecosystem services as biomass and carbon
and return back into atmosphere as soil respiration. In sequestration. However, sufficient data on all these
forest ecosystem, respiration is mainly dominated by aspects are limited and mostly aboveground biomass
soil respiration which accounts for about 60-80 per cent information is only available. Most biomass estimation
of the total respiration. The soil respiration has been studies involving Net Primary Production (NPP)
reported to account for carbon emission of >100 Pg estimation considers only the increment in aboveground
yr-1 globally and specifically in forests, may represent biomass (AGB) and litter fall, completely ignoring the
belowground biomass (BGB) component. For number of hours of sunshine in the month of November-
estimating ecosystem level NPP of a forest, time series December.
biomass data for tree, shrub, herb and litter components Key words: Dendro-climatology, Palaeoenvironment,
are pre-requisites. This data gap prompted us to Conifers, Tree-rings, Climate.
undertake this study in the tropical matured forests of
Meghalaya, North-Eastern India. We attempted to SI/O-18
study the species diversity, ecosystem biomass,
ecosystem carbon pool including aboveground, Understanding Interaction of Abiotic
belowground component, soil carbon, detrital biomass Variables on Net Primary Productivity
and NPP of the forest. The forest biomass was
estimated using aboveground and belowground in Indian Tropical Deciduous Forest
allometric models. The aboveground biomass,
belowground biomass, herb and shrub biomass and Soumit K. Behera1,2*, M.D. Behera2 and R. Tuli1,3
detrital biomass were 313.8 Mg ha–1, 50.82 Mg ha–1, 1
Plant Ecology and Climate Change Science Division, CSIR-
12.43 Kg ha –1 , 37.11 Kg ha –1 and 9.84 Mg ha –1 , National Botanical Research Institute, Lucknow-226001,
respectively. The tree diameter classes between >30- INDIA; 2Centre for Oceans, Rivers, Atmosphere and Land
80 cm girth classes contributed 202.75 Mg ha–1 and Sciences, Indian Institute of Technology, Kharagpur, INDIA;
3
31.56 Mg ha–1 contributing 64.61% and 62.1% to the UIET, Panjab University, Sector 25, Chandigarh, INDIA,
Email: soumitkbehera@gmail.com
total tree AGB and BGB. The total soil organic carbon
upto 1 m depth was 83.22 Mg C ha–1. The ecosystem Net primary productivity (NPP) represents a
biomass, ecosystem carbon, total aboveground NPP major component of net ecosystem production (NEP),
and belowground NPP in the forest were 374.51 Mg which is the net carbon (C) input from the atmosphere
ha–1, 265.52 Mg C ha–1, 12.26 Mg ha–1 yr-1 and 1.35 into the biosphere, the other component being the
Mg ha–1 yr-1, respectively. The tropical matured forest release of C through decomposition or combustion of
of the region showed higher carbon sequestration organic matter. NPP is considered to be the best
capacity in comparison to other forests. integrator measure of resource effects on ecosystem
Key words: Ecosystem biomass, Net primary production, processes. Patterns of terrestrial NPP may change in
Tropical matured forest, Carbon sequestration. response to changes in species association,
microclimate, CO2 concentration, natural disturbances
SI/O-17 and other environmental factors. Improved
understanding of plant functional types (PFT) level NPP
Tree Ring Analysis of Pinus roxbhu- may aid predictions of ecosystem response to ongoing
rghii from FRI, Dehradun climate and land-use changes. Modelling NPP in
tropical deciduous forests is very challenging and suffers
Sangeeta Gupta and Tarun* from data inadequacy and field measurements. Field
data on the interactions of tropical forest NPP to abiotic
Forest Research Institute, New Forest, Dehradun, factors are needed to resolve uncertainties among PFTs
Uttrakhand-248006, India, Email: tarunfri@gmail.com
particularly for seasonally dynamic deciduous
The study presents the dendro-climatic response ecosystems. Investigating the NPP patterns among
of Pinus roxbhurghii established in reserve forest of PFTs will help in assessment of carbon sequestering
Forest Research Institute estate. A collection of 3 tree- potential of these forests and further setting benchmarks
ring chronologies were studied for their dendro-climatic for carbon offset agreements among tropical countries.
response to changing temperature and precipitation. The present work investigates on the NPP patterns of
The various parameters like ring width, early wood, three PFTs {dry mixed (DM), sal mixed (SM) and teak
latewood were used to reconstruct the climatic variable. plantation (TP)} having distinct tree associations,
The tree ring chronology has a significant response to canopy stratification, different carbon assimilation rates
the minimum temperature of May-June. The ring and microclimate within a broad Indian tropical
chronology also had a significant correlation with the deciduous forest. We also tried to analyse the
biodiversity (represented by species richness, SR) and production and litter quality during exposure to e-CO2
productivity (NPP) interactions pattern emerging in which could significantly alter the input of nitrogen,
above three PFTs. Principal component analysis soluble phenolics, soluble sugars, lipids, cellulose, and
(PCA) and generalized linear models (GLM) were used lignin to soils, and so also the biogeochemical cycle of
to examine the relationship between individual the prevailing ecosystem.
structural, taxonomic and microclimatic variables Key words: FACE, Tropical species, Litter functional trait,
against NPP within individual PFTs. GLM analysis Mass loss, Nutrient flux.
revealed that leaf area index (LAI) as the best predictor
for NPP followed by air temperature among SI/O-20
microclimatic variables in all 3 PFTs.
Key words: Tropical deciduous forest, Leaf area index, Net Tree Community Regulate Soil
primary productivity, Plant functional types, Generalized Multifunctionality in a Tropical Dry
linear models.
Forest
SI/O-19
Ashutosh Kumar Singh1,2* and Nandita Singh2
Litter Functional Traits and Nutrient 1
Academy of Scientific and Innovative Research (AcSIR),
Dynamics in Tropical Trees Species CSIR-National Botanical Research Institute, Rana Pratap
Marg, Lucknow-226001, INDIA; 2Plant Ecology and
under Elevated CO2 Environment in Environmental Science Division, CSIR-National Botanical
India Research Institute, Lucknow-226001, INDIA, Email:
ashutosh.evst11@gmail.com
Apurva Rai1,2*, Ashutosh Kumar Singh1, Nandita Tropical dry forest covers large areas of tropical
Singh1 and Nandita Ghoshal2 region and characterized by a mosaic type of tree
1
Plant Ecology and Environmental Science Division, CSIR- communities that can be recognized as a co-dominant
National Botanical Research Institute, Lucknow-226001, multi-species sites to predominant mono-species. The
India; 2Department of Botany, Banaras Hindu University, previous forest management practices resulted in
Varanasi-221005, INDIA Email: apurvaraievs09@gmail.com, incorporation of some exotic species and recombination
ashutosh.evst@gmail.com, nanditasingh8@yahoo.co.in, of the forest vegetation. However, we know little about
n_ghoshal@yahoo.co.in the effect of multi-specific, mono- (here Shorea
Increasing CO2 potentially leads to changes in litter robusta, Hardwickia binate and Tectona grandis)
quality and litter input in forest soils. It is, therefore and an alien-specific (Lantana camara L.) sites on
vital to understand leaf litter traits in response to soil multifunctionality (provision of multiple ecosystem
increased atmospheric CO2. We conducted a field functions and services together that linked to soil). We
experiment to examine how the litter functional traits were assessed soil individual functions such as
and nutrient dynamics respond to e-CO2 concentration availability of soil nutrients (N and P), fractions of soil
in FACE (Free Air CO2 Enrichment) facility in CSIR- organic carbon (SOC) and glomalin related soil protein
NBRI, Lucknow, U.P, India. Litter was analysed for (GRSP), microbial biomass C, CO 2 efflux and
concentrations of carbon (C), nitrogen (N), soluble extracellular hydrolytic (acid and alkaline phosphatase,
sugars (SS), lipids, lignin, cellulose, hemi-cellulose (HC) â-glucosidase, dehydrogenase and fluorescein
and soluble phenolics (SP). We found that elevated diacetate) and oxidative (phenol oxidase and
CO2 significantly increased C/N, lowered litter N peroxidase) enzymes to topsoil (0-15 cm depth) and
concentration in leaf litter of Butea and Teak plantations, subsoil (15-30 cm depth) layers on the seasonal basis
respectively. Increase in litter biomass production (summer, winter, and rainy) in a highland TDF of India.
(Butea and Teak) under e-CO2, resulted in significant Soil multifunctionality index was calculated by averaging
increase in the flux of N, SS, SP and lipid into the soil. Z-scores of these parameters. We found that
Our study demonstrated the changes in litter biomass irrespective of soil depth and season, the extent of a
numerous soil individual functions and soil ecosystem services of tropical deciduous forests in
multifunctionality was higher at the multi-specific sites Northeast India, in general, and Kamrup district of
followed by mono- and alien-specific sites. It suggests Assam in particular. Hence, assessment of ecosystem
that under similar soil type and climatic condition higher services is extremely important to evaluate the impact
plant diversity promote soil multifunctionality. However, of ecosystem services on the livelihood of the local
in particular, we found that soil pH, alkaline phosphatase people.. Keeping above in account, present study aims
and phenol oxidase activity were higher at the alien- to assess the prevalent ecosystem services of the study
specific sites compared with native mono and multi area and examine peoples’ perceptions, knowledge and
specific sites. The multivariate (principal component attitude towards various services. Dominant services
analysis) analysis also showed the discrimination of alien related to utility were identified, categorised and ranked
specific sites from intermixed mono and multi specific following the standard methodologies. Drivers for
sites. These results suggest that L. camara sites alter change of ecosystem services were also identified,
the soil environment as they are very different from prioritized and analysed with emphasis on its impact
those created by native species. We concluded that L. on livelihood. A checklist of the plant diversity from
camara sites are least important in regards to promote the study site was recorded. Information about the
soil multifunctionality. Eventually, this study has broad condition of cultural services in terms of spiritual belief
implication in understanding revegetation of tropical was documented. Assessment of ecosystem services
forest aimed to expedite maximum ecosystem benefits. potential of selected forests was carried out with an
Key words: Glomalin related soil protein, Soil organic carbon, aim to strengthen the ongoing conservation efforts
Lantana camara, Soil enzyme, Hydrolytic enzymes, undertaken by the government, NGOs and local
Oxidative enzymes. community. Findings of the assessment would be helpful
for the decision makers to prioritise the forests for
SI/O-21 conservation purpose based on their ecosystem
services.
Ecosystem Services Assessment and Key words: Ecosystem services, Peoples’ perception, Drivers
Community Role in Conserving the of change in ecosystem services, Dominance, Conservation.
Services in Tropical Deciduous Forests SI/O-22
of Kamrup District of Assam in North-
East India Mainstreaming the Tropical
Underutilized Legume Winged Bean
Sangeeta Deka*, Om Prakash Tripathi and L.B. [Psophocarpus tetragonolobus (L.)
Singha
DC.] for Providing Household Food
Department of Forestry, North Eastern Regional Institute of
Science and Technology, (Deemed to be University), Nirjuli- and Nutrition Security
791109, Arunachal Pradesh, INDIA Email:
deka_sangeeta@yahoo.co.in, tripathiom7@gmail.com, Vinayak Singh, Arpit Chauhan, Shafquat Fakhrah,
lbsingha@hotmail.com Sagar Prasad Nayak, Kunwar Sarvendra and
Ecosystem services accentuate on the benefits Chandra Sekhar Mohanty*
that people derive from varied ecosystems (MA, Genetics and Plant Molecular Biology Division, CSIR-
2005a). These are mainly categorized into provisioning, National Botanical Research Institute, Lucknow-226001,
regulating, supporting and cultural services. Varied Uttar Pradesh, INDIA, Email: visiranj@gmail.com,
dimensions of human well-being namely basic human arpit12191@gmail.com, shafquatfakhrah@gmail.com,
needs, economic needs, environmental needs and sarvendrakr88@gmail.com, sagar.nayak51@gmail.com,
sekhar_cm2002@rediffmail.com
subjective happiness are a few particular benefits
derived from the ecosystems. Literature review Winged bean Psophocarpus tetragonolobus
revealed that there is paucity of empirical data on (L.) DC. is a tropical legume with high protein and oil
content. The plant is endemic to northeast India and is hunger, malnutrition, under nourishment, lack of
infested with the anti-nutrient condensed tannin (CT). adequate vitamins and minerals in their diet. Malnutrition
Leaf being the site of biosynthesis of condensed tannin among the children is rampant resulting anaemia a very
so, a comparative transcriptome analysis detected the serious problem throughout the world. Although, India
responsible transcripts and their corresponding enzymes has passed through various revolutions and achieved
in the biosynthesis of CT. Based on the similarity unprecedented development in the food crop
searches against gene ontology (GO) and Kyoto productivity and many crops have increased their
encyclopedia of genes and genomes database (KEGG) manifold productivity but the challenges of malnutrition
revealed 5210 transcripts involved in 229 different and threat of climate change continues vigorously.
pathways. A total of 1235 contigs were found to be Underutilized wild edible plants may sustain the effect
differentially expressed between high and low-CT lines of climate change; appear to be the crop of future and
of winged bean. There were 2237 simple sequence need focused attention as it can meet nutritional needs.
repeats (SSRs) in high-CT lines and 1618 SSRs in low- Present paper highlights some of the underutilized wild
CT lines. This study and its finding will be helpful in edible plants species like Basella alba, Bauhinia
providing information for functional and comparative purpurea, Boerahavia diffusa, Capparis zeylanica,
genomic analyses of condensed tannin biosynthesis in Crotalaria juncea, Diplazium esculentum, Ipomoea
legumes in general and winged bean in specific. A aquatica, Physalis minima, Portulaca oleracea,
detailed investigation on CT-biosynthesis is reported Alangium salvifolium, Anthocephalus chinensis,
for the first time in any underutilized legume crop Antidesma ghaesembilla, Averrhoa carambola,
through this study. Marker-assisted selection and Bridelia squamosa, Buchanania lanzan, Carissa
breeding between the diverse lines for mapping opaca, Cordia dichotoma, Dillenia pentagyna,
population development is underway. Validation of Diospyros exculpta, Ficus racemosa, F.virens,
specific genes and their further silencing through virus- Flacourtia indica, F.jangomas, Grewia hirsuta,
induced gene silencing is attempted with preliminary- Limonia acidissima, Madhuca longifolia,
level level of success. Regeneration of the plant from Manilkara hexandra, Pueraria tuberosa, Rumex
cotyledonary and nodal explants have successfully dentatus, Schliechera oleosa, Spondias pinnata and
optimized with an aim for establishing molecular Xeromphis uliginosa etc. as a future food crop provide
transformation of P. tetragonolobus. newer tastes, texture, aroma, flavour and recipe may
Key words: Winged bean, Anti-nutrient, Condensed tannin, sustain the effect of climate change for their sustainable
Transcriptome, Underutilized legume, Virus-induced gene utilization in commerce and trades need urgent attention
silencing. in human welfare.
Key words: Underutilized wild edible plants, Climate change,
SI/O-23 Future food plants, Sustainable utilization, Uttar Pradesh.
SI/P-2 SI/P-3
with two regimes of drought stress i.e. mild drought diurea (EDU) as a foliar spray (300 ppm). Average
(MD; 40-50% RWC) and severe drought (SD). It was ambient ozone concentration during the experiment was
found that the flower size of the plants grown in eCO2 61.6 ppb and average ambient and elevated CO 2
condition was larger as compared to their respective concentration (eCO2) during the experiment was 412.56
controls grown under ambient conditions. Biomass of ppm and 562.53 ppm, respectively. EDU treatment
T. cinerariifolium was found to be reduced during the resulted in less lipid peroxidation along with increased
MD and SD stress in ambient as well as in eCO2 chlorophyll content, biomass and yield. EDU alleviated
conditions. However, biomass reduction was more the negative impacts of ozone by enhancing activities
prominent in ambient ring as compared to eCO2 ring. of antioxidants and antioxidative enzymes. The present
Pyrethrin contents were found to be progressively study indicates that prevailing ozone concentrations in
increased during MD and SD respectively, under eCO2 and around Lucknow city have unfavorably affected
conditions. On the contrary its content was found to various physiological and biochemical characteristics
be significantly decreased in MD and again increased of two sorghum cultivars leading to significant growth
in SD as compared to its respective control in ambient reductions. EDU application protected plants against
conditions. The amount of pyrethrin was found to be harmful effects of ambient ozone whereas elevated
higher in ambient condition as compared to eCO2 . CO2 could ameliorate adverse effects of ozone. Two
Present study reveals that eCO2 condition helps plants cultivars selected for experiments responded
to adapt under drought condition(s) simultaneously differentially at either developmental stage of life cycle.
increasing the secondary metabolite production thus PHS-111 performed better in terms of growth and
increasing its economic value as medicinal plant. enzymatic activity over VHS-999. It indicates PHS-
Key words: Tanacetum cinerariifolium, Drought stress, 111was more responsive to EDU treatment and
eCO2, Pyrethrin, Secondary metabolite. (eCO2 ).
Key words: Tropospheric ozone, EDU, Forage crops, Fibre
SI/P-6 content.
drought conditions. Among 33 Trichoderma isolates different locations of districts Rawalpindi, Jhelum,
(NBRI-PR1–NBRI-PR33), NBRI-PR5 was selected Attock, Chakwal and Islamabad revealed maximum
after screening for stress tolerance, antagonistic activity mean disease incidence (MDI) on potato was recorded
against phyto-pathogens and P solubilization. The in Attock (37.4%) followed by Islamabad (35.8%),
selected strain, Trichoderma koningiopsis (NBRI-PR5) Jhelum (31.76%) and Rawalpindi (30.5%) while
was characterized and identified using ITS sequencing minimum in Chakwal (20.2%). Maximum MDI on
(Accession no. JN375992). Results show that NBRI- tomato was observed in Islamabad (38.7%) followed
PR5 uses different mechanisms of P solubilization under by Attock (36.3%), Rawalpindi (34.9%) and Chakwal
in-vitro alkaline and drought conditions. NBRI-PR5 (29.6%) while minimum in Jhelum (27.5%). MDI on
produced organic acids for solubilizing insoluble tri- chillies was maximum in Attock (30.9%), followed by
calcium phosphate (TCP) at high pH stress. In drought Islamabad and Jhelum (29.5%), while minimum in
conditions NBRI-PR5 accumulated poly phosphate in Chakwal and Rawalpindi (27.3%). Since the climate
its mycelia and produced alkaline phosphatase enzyme of the region has considerable temperature variations.
for P solubilization. The study concludes that T. Daytime temperature reaches above 40oC during the
koningiopsis employs different mechanisms of P summer while ranges between 0-10oC in winter. The
solubilization in different stress conditions and therefore, average annual rainfall is abundant at 1,249 millimetres
it can be used in management of stressed soils. (49.2 in). The fungus can remain active at a range of
Key words: Trichoderma, Abiotic stress, Phosphate temperatures and remains dormant as sclerotia. The
solubilization, Bioavailability. optimum temperature ranges 24-31oC for potato tuber
development, vegetative growth in chilli and the
SI/P-8 emergence of tomato seedlings also provide optimum
temperatures for R. solani disease development; 24-
Climate Change and the Current 32oC. In addition to temperature, soil moisture greatly
Scenario of Rhizoctonia solani Incited influences the amount of R. solani inoculum in the soil
that ultimately favours the disease development.
Disease of Solanaceous Vegetables Considering the changed climatic conditions, there is a
dire need to devise mitigation strategies for sustained
Amjad Shahzad Gondal*, Abdul Rauf and Farah vegetable production.
Naz
Key words: Rhizoctonia solani, Solanaceous vegetables,
Department of Plant Pathology, Arid Agriculture University, Maximum mean disease incidence (MDI), Climate change.
Rawalpindi, PAKISTAN Email: amjad@msu.edu,
amjadshahzad@live.com SI/P-9
Solanaceous vegetables; Potato (Solanum
tuberosum L.), tomato (Lycopersicon esculentum L.) Adaptation of Guar [Cyamopsis
and chilli (Capsicum annuum L.) are the dominant tetragonoloba (L.) Taub.] Plants in
vegetable crops worldwide that are generally cultivated
Response to Abiotic Stress of Light,
in the warm or tropical climate. They are grown
throughout the year in all parts of Pakistan. The quality Temperature and Drought
and quantity of the produce is directly affected by the
climate change as host plants and their pathogens are Shiv Narayan*, Komal Pandey and Pramod Arvind
prone to erratic climatic factors like temperature, relative Shirke
humidity, rainfall and CO2 prevailed during different Plant Physiology Laboratory, CSIR-National Botanical
crop seasons. Rhizoctonia solani is an important soil- Research Institute, Lucknow-226001, INDIA
borne fungal pathogen that is well adapted to various Email:shivnarayan2388@gmail.com, pashirke@nbri.res.in
environmental conditions. It causes black scurf, Guar [Cyamopsis tetragonoloba (L.) Taub.] is
damping-off, stem canker and root rot in these crops. an annual Kharif legume capable of growing under poor
Present study documents the scenario R. solani incited fertility and grown mainly in arid and semi-arid region
diseases in the Pothohar region of Pakistan. Survey of
of India. It is grown as a vegetable for human varieties grown under Free Air Concentration
consumption, forage, green manure and as a grain crop. Enrichment facility (FACE).The study would yield rice
The endosperm of guar seed contains galactomannan germplasms that are tolerant to anticipated climate
used as a food and non-food item. We studied the change as well as possible mechanistic understanding
performance of two guar varieties namely RGC-1002 of response of rice plants to combined stresses. The
and Sarada under different abiotic conditions. study would yield robust data about how rice
Photosynthesis in response to light, temperature and physiological, genetic and yield parameters will respond
water stress was measured in the month of May and to anticipated changes in the atmosphere which may
June. In photosynthesis Vs light response curve, we help improve modelling and breeding efforts in the
observe the maximum photosynthetic saturation at 1200 future.
µmol m-2s-1 of light in RGC-1002, whereas 800 µmol Key words: Ozone, Elevated CO2, Rice, Indo Gangetic Plains,
m-2s-1 of light in Sarada. While in temperature response, FACE.
the maximum photosynthesis ranged from 35-380C in
RGC-1002 and 28-30oC in Sarada. In water stress SI/P-11
condition, RGC-1002 (RWC= 68%) shows significantly
higher photosynthesis then Sarada (RWC= 58%). Our Interaction between Climate Change
studies suggest that RGC-1002 is better adopted for Drivers and Nutrient Fertility:
abiotic conditions of high light, high temperature and
water stress then Sarada at physiological level. Temperature, CO2, and Phosphorus
Key words: Guar, Photosynthetic Photon Flux Density, Water Vangimalla R. Reddy1* and Shardendu K. Singh1,2
stress, Relative Water Content.
1
USDA ARS, Crop Systems and Global Change Laboratory,
SI/P-10 Bldg 001, Rm 342, BARC-W Beltsville, MD, USA; 2School of
Environmental and Forest Science, University of Washington,
Impact of Elevated CO2 and/or O3 on Seattle, WA, USA Email: vangimalla.reddy@ars.usda.gov,
shardendu.singh@ars.usda.gov
Rice Varieties under Open Field
Climate change is already affecting the global
Conditions natural resource base that societies depend on to
provide food, fiber, fuel, and recreational services. An
Rushna Jamal*, Mitul Kotecha, Rekha Kannaujia,
increase in the global air temperature (T) and carbon
Vivek Pandey
dioxide (CO2) concentration is associated with the
Plant Ecology and Climate Change Science Division, alterations in atmospheric chemistry such as emissions
CSIR-National Botanical Research Institute, Lucknow of greenhouse gases (e.g., CO2, methane, nitrous oxide,
226001, INDIA, Email: and chlorofluorocarbons). Temperature, CO 2, and
Human activities are contributing to increase in nutrient availability are among the major determinants
the concentrations of atmospheric carbon dioxide (CO2) of crop’s adaptation that drive their productivity. In
and other greenhouse gases, including tropospheric natural settings, the productivity of an agro-ecosystem
ozone (O3). Indo-Gangetic plains (IGP) region is one depends on the intricate balance between multiple
of the most heavily populated and polluted region of environmental drivers. Lately, our understanding of crop
India. Rice is the most important cereal crop grown in response to individual environmental factors has
this region. There are reports of stagnation and even advanced significantly, but information on multiple
decline in rice production which has been attributed to interacting factors is needed to understand full impacts
many biotic and abiotic factors. We argue that elevated of climate change on crop productivity. Even though a
CO2 and/or O3 might be contributing towards these large proportion of crop productivity globally occurs in
yield losses. In the present experiment, we are assessing nutrient-limited conditions, crop response to climate
impact of elevated CO2 (550 ppm) and/or O3 (+20 ppb) change drivers has often been investigated under well-
on growth, physiological, biochemical, molecular, yield fertilized condition. Thus, the interactive impacts of
and nutritional quality parameters of twenty four rice temperature, CO2, and phosphorus (P) fertility on crop
productivity are still unclear. To fill in this knowledge- cycle helpful in maintaining the balance of atmospheric
gap, soybean was exposed to the combinations of two CO 2 concentrations and their response to
levels of each T (optimum and elevated, eT), CO2 environmental change. Increasing primary productivity
(ambient and elevated), and P fertilization (sufficient in tropical forests over recent decades has been
and deficient, dP) throughout the season. Results attributed to CO2 fertilization and greater biomass in
showed that dP was the most detoroius to soybean tropical forests could function as a substantial carbon
vegetative growths while eT was the most detrimental sink in the future. However, the carbon sequestration
to reproductive growth. The elevated levels of CO2 capacity of tropical forest soils is uncertain and
and temperature (separately or jointly) compensated, feedbacks between increased plant productivity and
at least partially, the detrimental impacts of P deficiency soil carbon dynamics remain unexplored. Soil CO2
on vegetative growth, such as biomass production. efflux is directly related to both microbial and root
However, compensatory effects of eT under P activities and are largely controlled by environmental
deficiency completely disappeared for the reproductive factors such as precipitation, soil moisture and
development, such as pod development. This indicated temperature. Soil CO2 efflux is a major component for
that plant response to combined stresses (e.g., eT+dP) assessing the carbon sequestration capacity of the forest
is exclusive, which might not be accurately inferred ecosystems as part of the global carbon cycle. The
from results obtained when stresses (e.g., eT or dP) present study was conducted in Katerniaghat Wild Life
imposed individually. Furthermore, the impacts of a Sanctuary (KWLS) in three forest communities (Teak
given stress situation might be even more complex due plantation, Sal mixed and dry mixed forests). Diurnal
to the contrasting nature of responses between plant soil CO2 flux (µmol CO2 m”2s”1) was measured in post
attributes (e.g., vegetative versus reproductive monsoon season using automated LI-COR 8100 soil
growth).The potential implication of these results might CO2 flux system (LI-COR, USA) with attached survey
be associated with the P-fertilizer management due to chamber (8100-103; 20 cm diameter). Variations in soil
its link with the agroecosystem and environmental water potential was measured using in Psypro (Wescor
pollution. Inc., USA). Soil was sampled seasonally from two
Key words: Abiotic stress, Climate change, Interaction, depths (0-15 and 15-30 cm) at three times intervals
Photosynthesis. (morning, afternoon and evening) in each forest
community for diurnal measurement of soil water
SI/P-12 potential. We tried to understand the interaction of soil
physical and chemical properties with soil CO2 efflux
Interaction of Soil CO2 Efflux with Soil in each forest community. We observed average efflux
Parameters in Three Different Forest rates of 3.04±0.39, 3.24±0.47, 3.41±0.72 (µmol CO2
m-2s-1) in Teak plantation, Sal mixed and dry mixed
Communities in Tropical Deciduous forests, respectively. Maximum total organic carbon
Forest of Northern India (TOC) was observed in Sal mixed forest (20.28 g kg-
1
) followed by dry mixed forest (19.90 g Kg-1) in post-
Shruti Mishra 1 *, Soumit K. Behera 1 , L.B. monsoon season. Soil physical properties (majorly bulk
Chaudhary1, M.K. Jain2 and Vipin Kumar2 density, particle density, porosity and water holding
1 capacity) are strongly correlated with soil CO2 efflux
Plant Ecology and Climate Change Science Division, CSIR-
National Botanical Research Institute, Lucknow-226001, in comparison to soil chemical properties (majorly total
India; 2Indian Institute of Technology, Indian School of organic carbon, pH, EC, total nitrogen and total
Mines, Danbhad-826004, India Email: mishrashruti. phosphorus). The soil physical properties revealed
1989@gmail.com strong positive control over soil carbon sequestration
in tropical deciduous forests of India.
Tropical forests containing 30% of global soil
carbon (C) are a critical component of the global carbon Key words: Soil CO2 efflux, Soil water potential, Tropical
deciduous forest.
the standpoint of its nutritive quality for ruminant species diversity by highest value of Shannon index
herbivores. Results showed that L. leucocephala is (2.1540). While EP was the community with very poor
tolerant to high ambient O3. species diversity indicated by lowest Shannon index
Keywords: FAOE, TroposphericO3, Leucaena leucocephala, (0.5611). The SAMF exhibited the highest equitability
Leaf nutrients, Fiber Content. index (0.7505), where the species were more evenly
distributed. The nMDS and Pearson’s correlation
SI/P-15 studies reveal that the assembling of different forest
communities is affected by the different environmental
Effect of Environmental Variables in variables such as, Total organic nitrogen (TON),
the Assembling of Tree Community in Electric conductivity (EC), Soil bulk density (BD), Soil
porosity (SP), Available phosphorous (P), Potassium
Tropical Moist Deciduous Forest of ion (K) and Water holding capacity (WHC) (edaphic)
Himalayan Terai and Air temperature (AT), Absolute air humidity (AAH)
and CO2 (climatic). The study also reported 23 site-
Omesh Bajpai1*, Venkatesh Dutta1, Rachna Singh2, specific species, which require proper conservation
L.B. Chaudhary3 and Jitendra Pandey2 measures due to their low ecological amplitude and
1 narrow range of distribution.
Department of Environmental Science, Babasaheb Bhimrao
Ambedkar University, Lucknow-226025, Uttar Pradesh, Key words: Trees, Phytosociology, Environmental variables,
INDIA; 2Centre of Advanced Study in Botany, Banaras Cluster analysis, nMDS, Dudhwa National Park, Tropical
Hindu University, Varanasi-221005, Uttar Pradesh, INDIA; moist deciduous forest.
3
Plant Diversity, Systematics and Herbarium Division, CSIR-
National Botanical Research Institute, Rana Pratap Marg, SI/P-16
Lucknow-226001, Uttar Pradesh, INDIA, Email:
omeshbajpai@gmail.com Emission of GHGS and Community
Tropical forests play a vital role in the global Level Physiological Profiles of Microbes
carbon and energy cycles. The region along the
is Altered by Soil Amendments
Himalayan foothills in northern India forms one of the
distinct eco-climatic regions of the world. The objectives Nishtha Mishra*, Pratibha Verma, Priyanka
of the study are to find out the different tree Chauhan and Aradhana Mishra
communities and summarize major edaphic and climatic
variables affecting the tree community assembling in Division of Plant-Microbe Interactions, CSIR-National
the tropical moist deciduous forest. The forests of Botanical Research Institute, Rana Pratap Marg, Lucknow-
226001, India, Email: mishramyco@yahoo.com
Dudhwa National Park (DNP), a good representative
of tropical moist deciduous forest of Terai region has This study was conducted to elucidate effects of
been selected to explore of edaphic and climatic long-term fertilization practices on plant growth, GHGs
variables. The cluster and non-metric multidimensional emission (methane and carbon dioxide), and microbial
scaling (nMDS) analyses grouped the forests of DNP diversity in rice field soil. The rice field selected for
into five major forest types: Sal miscellaneous forest experiments represented balanced and imbalanced
(SMF), Sal-Asna miscellaneous forest (SAMF), Low fertilization practices. Among five treatments [Control
land miscellaneous forest (LMF), Teak plantation (TP) (SC), 100% urea (SU), NPK-Zn (NPK), 50% NPK-
and Eucalyptus plantation (EP). Each forest type has Green manure-biofertilizer (NGMBF) and biofertilizer
its specific species composition and dominance. The (BF)], a significant increase in root growth parameters
monoculture nature of EP was clearly indicated by was observed in NGMBF treatments which was
highest dominance index (0.7566) followed by TP with closely followed by BF treatments. Similarly, further
dominance index 0.4963. Whereas the lowest analysis of yielding attributes also exhibits the same
dominance index (0.1659) with highest Simpson index pattern of growth where an increase in different yielding
(0.8341) was computed for LMF, indicating its highest parameters was evident in NGMBF treatment. Soil
heterogeneity. The LMF also showed the highest incubation studies showed highest CH 4 and CO 2
emission on day3 with NPK treated soil in submerged recorded in the BF treatment which was followed by
condition whereas it was least in BF treated soil. NGMBF, SU and NPK. These findings suggest that
Dehydrogenase activity in soil was also analyzed and sustainable management practices like amendment
it was recorded highest in BF treated soil. The structure with BF in the soil are needed to mitigate CH4 and
of the microbial community in different soil amendments CO2emission from rice cultivation.
was analyzed through the sole-carbon-source utilization Key words: Climate change, Fertilizer, Methane emission,
profiles using ECO Biolog plates. As compared to SC, Soil microbial diversity.
maximum changes in the community structure was
Session II
Biodiversity: Prospection and Conservation
insensitive to high-CO2, UV-B or nutrients and were Humanity, along with all species, is dependent on
mostly temperature dependent. Since crop growth in the planet earth. Degradation of our environment will
inderminate crops such as cotton is very plastic, affect the carrying capacity of life on the planet and
additional carbon available in a high-CO2 environment this in turn will affect the number of people that the
favored more vegetative and reproductive growth planet can support. Many of the natural resources of
under optimal conditions. Crop reproductive processes, the planet when degraded have impacts far beyond
however, were very sensitive to both higher and extreme what is commonly perceived. Due to the large body of
climatic conditions predicted in future climate (higher research that has been done, it is now clear that many
and more frequent episodes of temperatures and UV- of the world’s ecosystems are under intense pressure
B radiation). Elevated CO2 did not ameliorate the from human encroachment, the harvesting of natural
damaging effects of either higher temperatures or UV- resource and land clearing. This pressure is causing
B radiation on processes related crop yield. More the destruction or degradation of habitat and species
recently, we have been exploring genotype by and permanent loss of productivity, threatening
environment interactions to understand the variability biodiversity and with it human well-being. The
and to assist the breeders select the best lines suited in resilience of many ecosystems is likely to be exceeded
a changing climate in their breeding programs. this century by an unprecedented combination of
Key words: Global climate change, Anthropogenic activities, climate change, associated disturbances like flooding,
Food security, Future environments, Breeding programs, drought, forest fire, insects, ocean acidification and other
High-CO2-grown plants global change drivers such as land use/cover change,
pollution, over-exploitation of natural resources.
SII/L-4
International protocols require regular monitoring,
conservation, and sustainable utilization of biodiversity.
Plant diversity characterization using India has done some exercise under NBSAP
satellite remote sensing programme though it didn’t result in a seamless
countrywide biodiversity spatial database. We took this
S.P.S. Kushwaha challenge under a jointly funded programme of the
Indian Council of Forestry Research and Education, Department of Space and Department of
Dehradun-248006, Uttarakhand, INDIA, Email: Biotechnology, Government of India and used remote
spskushwaha@gmail.com sensing and GIS technology effectively. In process, we
also made use of landscape parameters such as
Biological diversity means the variability among
interspersion and juxtaposition and multi-criteria
living organisms from all sources including, inter-alia,
analysis together with field inventory of plant diversity.
terrestrial, marine and other aquatic ecosystems and
A total of 16,577 field plots were laid in different
the ecological complexes of which they are part; this
vegetation types across India. Even species utility was
includes diversity within species, between species and
taken into account. It took 10 years to complete this
of ecosystems. Amazing forest diversity- 16 Type
mammoth job with high precision. Today country has
Groups, 46 Sub-Groups and 221 Ecologically Stable
wall-to-wall natural vegetation types, disturbance
Natural Vegetation Formations. One of the 18 mega-
diverse countries (ranked between 10th and 12th) with regimes, fragmentation and plant richness database.
No such survey has been carried out by any country
four global biodiversity hotspots (Himalaya, Indo-
of the world. Needless to mention that this database
Burma, Western Ghats and Sri Lanka, and A & N
has provided much needed impetus to biodiversity
Islands). Accounts for 7.8% of recorded species: 45,968
conservation efforts in the country.
recorded species of plants & 91,364 species of animals
comprising 11.18% flora and 7.44% fauna globally. With Key words: Plant diversity, Satellite remote sensing, GIS,
only 2.5% of the world’s land area, supports 16% of Ecosystem
world’s population 18 % of the domestic cattle
population.
SII/O-1 SII/O-2
would be facilitated some woody and shrub species in of Broknes Peninsula. Altogether 16 species of
boreal zone of Canada. Cyanoprokaryotes, belonging to 09 genera were
Key words: Climate change, RCP, Machine learning recorded from diverse habitat of Broknes Peninsula of
Larsemann Hills, East Antarctica as new record from
SII/O-3 this area.
Key words: Diversity, Cyanoprokaryotes, Broknes Peninsula,
Diversity of Cyanoprokaryotes from Larsemann hills, Antarctica.
Broknes Peninsula of Larsemann Hills,
SII/O-4
East Antarctica: First Report
Assessment of genetic diversity using
Pratibha Gupta
ISSR and SCoT markers in natural
Botanical Survey of India, Ministry of Environment, Forest
& Climate Change, Government of India, AJCBIBG, Botanic populations of Ormosia robusta Baker-
Garden, Howrah-711103, INDIA, Email: drpratibha2011@ a critically endangered species from
rediffmail.com
Northeast India
Antarctica is an extreme environment and
contains many unique geological, glaciological and R.K. Nilasana Singha1, Ashiho A. Mao1*, Deepu
biological features and it is a gift of nature in natural Vijayan1 and S.K. Barik2
forms but due to changes in climatic conditions, we 1
Botanical Survey of India, Eastern Regional Centre,
can see the effect of Global environmental changes in Shillong-793003, INDIA; 2National Botanical Research
Antarctica. The Larsemann Hills area are located Institute, Lucknow-226001, INDIA, Email: nilasana@
approximately halfway between Vest fold Hills and gmail.com, aamao2008@gmail.com
Amery Ice Shelf on South-eastern coast of Prydz Bay
which includes two main peninsulas, the western named Ormosia robusta is a perennial tree species
Stornes and the eastern named Broknes. Human belonging to the family Fabaceae. The species is
activities in this area are promoted by the coastal location critically endangered and has been reported from India,
and ice free landscape. The further scientific research Bangladesh, Myanmar, and Thailand. In India, this tree
and the potential for tourist visits may affect the species was reported from Arunachal Pradesh and
cyanoprokaryotes (cyanobacterial) diversity. The Assam. Recent field surveys led to the discovery of
Antarctic environment is highly sensitive and new distributional records of this critically endangered
susceptible to the impacts of human activities and has species from Itanagar and Pasighat (Arunachal
much less natural ability to recover from disturbance Pradesh), Balpakhram and Baghmara (Garo hills,
than the environment of other continents. These Meghalaya) and Lengpui (Mizoram). Because of its
changes ultimately affect the growth and diversity of small population size with only ten individuals, there is
Cyanoprokaryota. Cyanobacteria are ubiquitous, a possibility of low genetic variability among the
microscopic, inhabiting the world’s oceans, freshwater individuals. It is therefore essential to study the genetic
bodies, soils, rocks, etc. and are responsible for most variability within as well as among different populations,
of the global production of organic matter by to have an insight into the possibility of genetic drift. In
photosynthesis. Thus, they play a fundamental role in the present study, we used inter-simple sequence
the world’s ecosystems and their kaleidoscopic repeats (ISSR) and started codon targeted (SCoT)
diversity, systematic and phylogeny is indispensable. fingerprinting to study the genetic diversity of inter-
Cyanoprokaryotes play a very important role in and intra-population variability of O. robusta collected
monitoring the water quality and there are prospects from five locations. The screening was carried out using
of utilisation of cyanoprokaryotes genetic stock in 20 ISSR and 20 SCoT primers. The clear and
multiple significant ways. As Stornes peninsula comes reproducible DNA fragments generated from 17 ISSRs
under the Antarctic Specially Protected Area (ACPA) and 16 SCoT primers, ranged from 200 bp to 800 bp.
hence the samples were sampled from diverse habitat The profiles of all the ten plant samples collected from
five locations viz., Itanagar (1), Pasighat (2), Baghmara systems, and shared species and finally to create
(1), Balpakhram (5) and Lengpui (1) displayed a high wetland reserves.
level of polymorphism among them, indicating wide Key words: Environment, Inter-governmental, Ramsar
genetic variation, albeit their small number. convention, Treaty, Wetlands.
Key words: Critically endangered species, Ormosia robusta,
inter- and intra- population diversity, ISSR and SCoT markers SII/O-6
genera based on ABGD and TaxonDNA analysis. Our discoveries before getting adversely affected by global
analysis underscored the efficacy of matK + ITS2 climate change and anthropogenic influences.
markers with atpFatpH as the best combination for Key words: Phycopeltis, Phyllosphere, Rural-natural forest,
mangrove identification in the west coast India region. Semi-urban, Trentepohliales.
Key words: DNA barcoding, Mangroves, West coast, India.
SII/O-8
SII/O-7
Status and Distribution Pattern of
Leaf Colonising Algae-Phycopeltis Native and Endemic Species on the
spp. (Trentepohliaceae, Chlorophyta) Shimla Water Catchment Wildlife
from the Southern Western Ghats, Sanctuary of Western Himalaya
Kerala, India
Dipika Rana1, Kulraj Singh Kapoor2 and Anupam
Binoy T. Thomas*, M.V. Bhagya and V.P. Thomas Bhatt1
1
Phycotechnology Laboratory, Department of Botany, CSIR-Institute of Himalayan and Bioresource Technology,
Catholicate College, Pathanamthitta, Kerala, INDIA Palampur-176061, Himachal Pradesh, INDIA; 2Himalayan
Email: bttkripa@gmail.com Forest Research Institute, Panthaghati, Shimla-171009,
Himachal Pradesh, INDIA Email: dipikahfri@gmail.com
The study is the pioneer investigation of leaf
colonising algae, Phycopeltis spp. from the tropical Invasions and endemism represents the two most
ecosystems of Kerala, India. Phycopeltis is the most extreme conditions of the possible geographical
abundant and distinct representatives of epiphyllous distributions of species. The areas with high percentage
algae in the tropical ecosystems. The genus of native and endemic species indicate the high
Phycopeltis was established by Millardet and it belongs conservation value of that particular area. The bio-
to the order Trentepohliales, class Ulvophyceae and geographic provinces with high diversity of native and
the division Chlorophyta. Presence of â-carotene and endemic species show the pristine ecosystems with
haematochrome are the unique features of the order least disturbance, whereas low richness of the sensitive
Trentepohliales. While possessing a remarkable biodiversity elements indicates high disturbance and
diversity in the tropical ecosystems, the genus opening the way for the invasion of non-natives.
Phycopeltis appears under-described in this region. Therefore, the native and endemic plants in a natural
Nine species of Phycopeltis from the semi-urban and ecosystem play an important role in the conservation
rural areas of Pathanamthitta District, Kerala, India and preservation of an ecosystem. In the present times
were isolated such as P. arundinacea (Montagne) De the high anthropogenic pressure and climate change
Toni, P. irregularis (Schmidle) Wille, P. epiphyton have increased the stress on natural ecosystem and
Millardet, P. flabellate Thompson and Wujek, P. aurea leading to rapid loss of native species and proliferation
Karsten, P. vaga Thompson et Wujek, P. treubii of non-native species. Maintenance of naturalness
Karsten, P. treubioides Thompson and Wujek and P. (nativity) and uniqueness (endemism) of the ecosystem
minuta Thompson and Wujek. It was found that species has become a big challenge. The extinction of the
richness and diversity indices of Phycopeltis spp. were indigenous species has caused biodiversity reduction,
more prevalent in the natural forest and in monsoon habitat loss and alteration of natural ecological
seasons; limited in the region of semi-urban areas and processes. Introduced species pose threat to
summer season. Phycopeltis was more widespread biodiversity by direct competition with native and
on the adaxial side of the moist leaves during all endemic plant species. The present study was
seasons. The study confirms the diversity of epiphyllous undertaken in the Shimla Water Catchment Sanctuary
Phycopeltis spp. in the tropical ecosystem. The of Western Himalaya in which out of the total reported
detailed surveys of leaf colonising Phycopeltis spp. 476 species, 245 species were native to the Indian
are desirable and can be expected to find new Himalayan Region (IHR), remaining 231 species were
non-natives, while 8 species were endemic and 85 literature. Total 35 persons were selected for the study
species were near endemic to the IHR. Total 312 and information of about 25 plant species was gathered.
species were quantified, of which 53.85% species were Out of 25 plant species, maximum number of plant
native, while 46.15% were non-native. 20.19% of the species recorded was herbs followed by shrubs, trees
total species were near endemic and 2.56% of the total and climbers. Leaves comprised the major plant part
was endemic to the IHR. 71.43% trees, 66.15% shrubs used. Information gathered can be used in search of
and 47.94% herbs were natives, whereas among these new herbal medicines by the researchers in the field
native species, 40.00% trees, 27.90% shrubs and of modern drug development.
29.52% herbs were near endemic. Among the native Key words: Traditional knowledge, Healthcare, Kapurthala,
species 4.65% shrubs and 3.80% herbs were endemic Punjab.
species. The altitudinal distributions of the native, non-
native and endemic species were also evaluated. SII/O-10
Key words: Indian Himalayan Region, Catchment, Sanctuary,
Endemic, Near Endemic, Native. Flower Traits and its Variability as an
Adaptive Strategy for Augmenting
SII/O-9
Reproduction in Plants Growing in
Traditional Knowledge on the Utiliza- High Altitude Environments
tion of Plant Species for Health Care 1 1
Mustaqeem Ahmad *, Sanjay Kumar Uniyal ,
Jaskirat Kaur*, Rajinder Kaur and Avinash Kaur Daizy R. Batish2 and H.P. Singh3
Nagpal 1
High Altitude Biology Division, CSIR-Institute of Himalayan
Department of Botanical and Environmental Sciences, Guru Bioresource Technology, Palampur-176061, HP, INDIA;
2
Nanak Dev University, Amritsar-143005, Punjab, INDIA Department of Botany, Panjab University Chandigarh-
Email: jaskiratk888@gmail.com, swab2002@yahoo.com, 160014, INDIA; 3Department of Environmental Studies,
avnagpal@rediffmail.com Panjab University Chandigarh-160014, INDIA
Email: mustaqeem.env@gmail.com, suniyal@ihbt.res.in,
The present study was aimed at exploring the daizybatish@yahoo.com, hpsingh_01@yahoo.com
traditional knowledge of local inhabitants of Kapurthala
Flower traits are key to reproductive success of
district on the utilization of plant species for health care
a plant species. Along an altitudinal gradient, owing to
management. Since times immemorial, plants have
limited growing season and thereby pollination success,
been used for various purposes by human population.
flower traits become crucial. Therefore, flower display
About 80% of the total world population is depending
area (FDA), flower longevity and resource investment
upon plants for their health care. The traditional
should be higher in plant populations growing at
knowledge of local inhabitants has been passed from
higheraltitudes as compared their low altitude
generation to generation mostly through oral
counterparts. We hypothesized that altitudinal variation
communication and in doing so part of it might be lost.
of flower traits handles reproductive success of plants.
So there is growing interest in documentation of
To test this, a study was conducted along an altitudinal
traditional knowledge. People rely on plants for many
gradient of 2000 to 4000 m asl in the Dhauladhar
health problems like fever, cough, diarrhea, constipation,
mountain range of western Himalaya during May
cuts and wounds etc. This study was based on
2015to September 2017. Along this altitudinal gradient,
questionnaire based personal interviews, field study and
twenty-one, 20×20 m permanent plots were established,
direct observations. Successive field surveys were
one each at every 100 m rise in altitude.Twenty-five
carried out from April 2015 to May 2016 mainly in
plant species were identified and 10 to 20 individuals
search of traditional healers or practioners. Information
of each were collected from the marked plots. Only
was also gathered from house wives, farmers, teachers
healthy looking, fully mature plants were collected for
and elderly people of the study area. Information was
flower trait (display area, mass and flower count)
also cross checked for authenticity from the available
analyses. After fruiting, seed count and seed mass were
also recorded for the selected 25 species. These were on the protection of Chure through initiation of
used as indicators of reproductive fitness. The ‘Rastrapati Chure Conservation Program’ since 2009.
relationship between altitude and traits for every It is an attempt to identify the problems, challenges
population sample were determined with a Pearson’s and issues of Chure forest conservation and to propose
parametric product movement(r) test at 95% an effective conservation plan using qualitative as well
confidence. To test the effect of elevation on as quantitative methods of analysis. Chure region has
reproductive traits, linear model of ANOVA was used. many opportunities of employment and income
The results revealed that except for specific flower generation through establishment of environment
area and seed count, reproductive traits positively and friendly green enterprises. Thus it is an urgent need to
significantly correlated with altitude. Seed traits were formulate short-term to long-term strategies with policy
associated with flower traits wherein FDA and flower priorities actions and result-oriented efforts in order to
mass were of prime importance. Increased flower establish Chure as a rich bio-diversity zone, with
visibility seems to ensure pollination while larger seed hazardless and improved livelihoods of its people. To
size in populations of high altitude species gives them a bridge this research gap, this research is on Community
chance to survive in harsh environments. We suggest and Collaborative Forestry contributing role toward
flower traits variations along altitude to be helpful for Chure development and Conservation completed. The
reproductive success of plants. paper discusses how development and conservation
Key words: Himalaya, Flower traits, Altitude, Reproductive initiatives carried out in the chure areas from the
fitness, Adaptations. Community based Forest Management practice. This
paper also principally discusses the lessons learned on
SII/O-11 Community and Collaborative Forests complementary
for Chure development/conservation to fulfill the broad
Impact of Community based Forest objectives of the President Chure Terai Madhesh Plan
Management (Community/Collaborative and millennium development goals. Based on the
conclusion that action research several recommenda-
Forestry) System in the Chure region tions for better management of Community based
Conservation and Development of Nepal Forest. At present forestry database show that 2.00
million households or 37 % of the population of Nepal
Damodar Sharma is involved in community forestry management program,
19361 Community Forestry User Groups (CFUGs)
Democratic Foresters’, Association of Nepal, Kathmandu,
NEPAL, Email: damodar.dfan@gmail.com have been formed 1,813478 hectares of National forest
have been handed over as community forests and
There is a growing concern regarding the 2,250,858 households have benefited from CF. It has
conservation and development of Chure hill of Nepal, been estimated that 3.75 million hectare of forest land
although land degradation of the Chure area of Nepal of Nepal can be put under Community Forestry.
is primarily contributed by different types of landslides Whereas there are 28 Collaborative Forest
and mass wasting phenomena including flooding and Management groups, who are managing more than
inundation. Landslides cause a huge loss of human life 71,000 ha Forest. There are three major partners
and property as well as environmental degradation in responsible to protect and manage Terai forest,
Nepal requiring extra resources for relief and recovery. including Department of Forest (DOF), local
Chure region is extended up to India, Bhutan & government and local communities in CFM. CFM is a
Pakistan from Nepal, covering 33 districts of Nepal concept, which has emerged after the 2000 A. D. It
with 12.6 % of country area. It is the youngest mountain has been estimated that more than 4.5 million hectare
of the world, and suffering from mass erosion, of forest land of Nepal can be put under Community
landslides and other environmental externalities which based Forestry. Currently, this management system is
make the region vulnerable. The ecological niche in recognized by forest policy, 2015 and Forest Act (1993)
and around the region makes the region more sensitive 2nd amendment 2017. Community based Forestry
and fragile. Government has given greater emphasis Management is being tools to address the issues of
users including nearby as well distant users, who have garden size and elevation range; generally the small
as well have not access and control over national forest gardens had maximum species diversity than the larger
since last 7 decades because of government policies garden while lower altitude had higher species richness
and programs. At the same time, there are numbers of than the higher altitude. The multistrata nature of most
issues and challenges emerged due to the unclear roles home gardens further reveal their ecological
and responsibilities among three partners. The newly significance in providing suitable niches to various
enacted “Local Government Operating Act 2074” has species at different altitudes. The species that were
also created confusion in the operation of Collaborative grown in the gardens, to some extent, were related to
Forest Management. Likewise, benefit sharing ratio is the socio-cultural and demographic factors of the home
also not clear so far. According to the New Constitution, garden owners and associated traditional knowledge
numbers of laws are being formulated or in the process in managing these resources. Many of the landraces,
of amendment. Capacity building, local level rare and endangered species were found domesticated
development, livelihood promotion as social in the garden. These findings reveal that home gardens
development; Income generation activities through are production system maintained purposively for
forest base enterprises with employment generation harvesting diverse products for food and nutritional
as economic development and water source and soil security and are important avenues for species
conservation as well as alternative energy as conservation at household level.
environment conservation were found fruitful in the Key words: Homestead agroforestry, Biodiversity
Chure and surrounding Terai area development. conservation, Nutritional security, Mizo community.
Key words: Socio-economic Impact, Development spectrum,
Community based forestry, Community Forestry, SII/O-13
Collaborative Forestry, Chure Conservation.
Medicinal Plant Diversity in Chandra
SII/O-12 Prabha Wildlife Sanctuary, Chandauli
Traditional Agroforestry for District, Uttar Pradesh
Biodiversity Conservation, Food and Nitisha Srivastava* and Achuta Nand Shukla
Nutritional Security of Mizo Botanical Survey of India, Central Regional Centre, 10,
Community of Northeast India Chatham Lines, Allahabad-211002, Uttar Pradesh, INDIA
Email: srivastava_nitisha@yahoo.com
Uttam Kumar Sahoo* and Soibam Lanabir Singh India is floristically rich country and is recognized
Department of Forestry, School of Earth Sciences & Natural in twelve mega biodiversity centers in the world. It is
Resource Management, Mizoram University, also blessed with rich medicinal plant diversity. Forests
Aizawl-796004, Mizoram, INDIA, Email: uksahoo_2003@ are the major sources of medicinal plants; besides these
rediffmail.com, uttams64@gmail.com they are also cultivated. Protected areas play a very
We evaluated 137 home gardens for their species significant role in conservation of medicinal plants and
composition and product outputs and use by the Mizo traditional knowledge. Chandra Prabha Wildlife
community, the study covered five districts of Mizoram. Sanctuary (CPWLS) is situated in the district
As many of 460 plant species belonging to various user Chandauli. The presented study was carried out in the
groups were encountered in the study, the majority of area of CPWLS for survey and collection of medicinal
the plant species belonged to vegetables, fruits and plants. Information on medicinal properties of plants
medicinal plants followed by firewood, ornamental encountered in the present study was generated using
species, timber and other category. Many other utility various relevant literatures. A total of more than 120
species such as spices, beverages, broom, handicraft medicinally important plant species were reported.
etc were also widely reported from the gardens. Medicinal plants of CPWLS belong to various families.
Nevertheless the composition of species varied with Out of these many plant species fell into the rare,
Bougainvillea is one of the most colourful, Whether invasive species vary in morphological
drought resistant and pollution tolerant climbing shrubs. traits and at molecular level across native and non-
Their colourful bracts make the Bougainvillea as the native regions, though much speculated, is not
preferred choice for the landscaping of parks and supported by adequate quantitative data. Furthermore,
gardens in tropical and sub-temperate regions and the origin of differences in traits influencing competitive
known as ‘Glory of the Tropics’. Bougainvillea is the success between exotic and native wild populations of
member of the family Nyctaginaceae. Bougainvilleas alien species is subject of debate. Therefore,
have attained popularity due to their bracts, which deciphering the driving factors among-population
appear in various colours and shades. The plant was divergence and the relative contribution from natural
first collected by a French Botanist, Commerson in selection and neutral genetic differentiation is important
1769 from Rio de Janeiro (Brazil). Commerson named in evolutionary biology. On the basis of morphometric
the plant after Lois Antoine de Bougainville the French traits, we compared five putative native populations of
Navigator, with whom he went on a voyage round the Phragmites australis in Quebec, Canada (haplotype
world. History of Bougainvillea is ca 250 years old. QN), six putative populations exotic to Quebec
It has travelled a long distance from Brazil to India. (haplotype QE), and five populations invasive in
There are ca 350 Bougainvillea cultivars of Kashmir, India (haplotype KE), for seven quantitative
Bougainvillea all over the world. 50 % of the traits. CpDNA PCR-RFLP analyses identified only one
Bougainvillea cultivars have been evolved in India. putative native P. australis population in Quebec. Using
India has been recognized as the International ten chloroplastic DNA microsatellite loci, we also
Registration Authority for Bougainvilleas by the compared nine populations each of haplotypes KE and
International Society for Horticulture. CSIR-National QE, and one population of haplotype QN. Among- and
Botanical Research Institute (NBRI), Lucknow is within-population variation was significant for all
maintaining an unique rich germ-plasm collection of measured traits, except for leaf width.Variation between
250 species/cultivars in the Botanical Garden. NBRI haplotypes was highly significant for leaf width.
is well recognized for its R & D work on Invasive haplotypes showed the highest values for the
Bougainvilleas. Institute has evolved ca 30 novel characters that were studied. Phenotypic differentiation
Bougainvillea cultivars, which remain in high demand among populations was moderate to high. The ten loci
in the floriculture industry. Bougainvillea has flourished were variable, and moderate levels of genetic diversity
very well in India than its centre of origin. were found within populations. Moderate genetic
differentiation was observed between haplotypes and
Key words: Bougainvillea, Commerson, Brazil, Louis Antoine
de Bougainville.
populations, indicating occurrence of gene flow correlation between species density (-1.0) and
between populations. Principal coordinates analysis frequency (-0.81) with total carbon density. However,
clearly distinguished three haplotypes. Phenotypic strong positive correlation (0.74) was observed
divergence exceeded genotypic divergence, indicating between basal area and carbon density reflecting the
divergent selection amongst populations. The invasive contribution of the forest with higher girth trees towards
populations in Kashmir and Quebec harboured highest biomass and carbon stock which further necessitates
proportion of common alleles of comparable size, and the conservation of such forests for carbon
belonged to similar haplotypes. Furthermore, their sequestration.
polyploidy nature suggests the European nativity of Key words: Natural resources, Carbon sequestration,
Kashmir populations. Biomass and carbon, Forest types, Conservation.
Key words: Common reed, CpDNA microsatellite loci,
Haplotypes, Phenotypic and genetic differentiation, Plant SII/O-17
invasion.
Environmental Determinants of Plant
SII/O-16 Richness in the Eastern Himalaya
Species Diversity and Carbon Stock in Rajendra Mohan Panda* and Mukunda Dev Behera
Three Temperate Forest Types of Garhwal Centre for Oceans, Rivers, Atmosphere and Land Sciences
Himalayas, Uttarakhand, India (CORAL), Indian Institute of Technology, Kharagpur,
West Bengal, INDIA, Email: rmp.iit.kgp@gmail.com
Siddhartha Kaushal and Ratul Baishya* Understanding underlying causes of high plant
Department of Botany, Faculty of Science, University of diversity is imperative for mountain ecosystems. The
Delhi, Delhi-110007, INDIA Email: kaushal.siddhartha@ study on the Indian Eastern Himalaya assesses effects
gmail.com, rbaishyadu@gmail.com of physiographic, climate, edaphic and disturbance on
With immense pressure on natural resources due plant richness of a national database. 1475 species from
to unsustainable rapid development and anthropogenic 411 geospatial locations between elevation ranges 129
activities, climate change is the worst fear of the and 4412 m were considered. Eight out of 35
century. The exponential carbon emissions have environmental variables were selected after optimisation
prioritized our goals to curb this rise and thus Carbon involving multicollinearity test and principal component
sequestration is nature’s answer to this problem. The analysis. Generalised additive model (GAM) cross-
state of Uttarakhand has 45.43% forest cover and offers validation results were validated using Ordinary least
various forest types because of climatic and geographic squares and structural equation model (SEM) for
variations offered by the Himalayas. The present study independent and multivariate analysis, respectively.
aims to report the species diversity in three temperate Potential evapotranspiration is found to have a
forest types (2144-2285m) of Garhwal Himalayas along significant climatic predictor, with an explained deviance
with tree species biomass and carbon stock. The stem of 22.5% and showed positive correlation with plant
density was observed between 275-360 trees ha-1 with richness. Precipitation seasonality and temperature
total tree biomass density ranging between 480 to 859 seasonality displayed negative correlations with plant
Mg ha -1 and corresponding carbon stock density richness. Elevation could explain 21.9% deviance,
between 220-397 Mg C ha -1 . Species dispersal for whereas cation exchange capacity could describe the
majority of the species in all the three forest types was maximum deviance by any variable i.e., 24%. The best
of contagious type. Simpson’s dominance index showed model could describe 51.1% in plant richness with
strong positive correlation with both elevation (0.79) contributions from climate, physiography, soil and
and total carbon density (0.78), while species richness disturbance. SEM grossly underestimated the GAM
(SR) showing negative correlation with both indicating results and described 26% variance of the best model
reduction of diversity with altitude. The indicating superior execution of nonlinear settings to
phytosociological data indicated a net negative species-environment relationships. Decision tree
described increase in temperature seasonality (> 476.8 0.50. This indicates the high acceptance of these plants
CV) could enhance plant richness at PET > 1163 ha in the primary health care. We also discussed the
yr-1. Precipitation seasonality (d” 82 CV) could increase degree of invasiveness and habitat preference of these
plant richness with decrease in temperature seasonality species. The use of invasive alien plant species relieves
(d” 476.8 CV). Precipitation seasonality (value > 109 the pressure on the native plant species that leads to
mm) and precipitation (> 1719 mm) could increase plant the native plant diversity conservation. Finding of this
richness with rise in temperature seasonality (> 463.6 study can be used as an ethnopharmacological basis
CV). Precipitation seasonality (> 93 CV) and for selecting plants for future phytochemical
precipitation (> 2983 mm) could reduce plant richness pharmacological studies.
further, even at low level of temperature seasonality Key words: Ethnomedicine, Invasive, Tribal, Jhabua.
(463.6 CV). It infers that climate defined by water-
energy dynamics and its heterogeneity act in synergy SII/O-19
in describing plant richness. Comparison of different
modeling approaches is suggested to crosscheck Aconitum duclouxii H. Lév.
validity of model selection.
(Ranunculaceae)- A New Record for
Key words: Eastern Himalaya, Plant richness, GAM, SEM.
the Indian Flora
SII/O-18
Danish Husain1,2*, Priyanka Agnihotri1,2and Tariq
Assessment of the Diversity of Invasive Husain1,2 *
1
Alien Plants and its Role in Ethno- Plant Diversity, Systematics & Herbarium Division, CSIR-
National Botanical Research Institute, Rana Pratap Marg,
medicine in Western Madhya Pradesh, Lucknow-226001, INDIA; 2Academy of Scientific and
India Innovative Research (AcSIR), Ghaziabad-201002, INDIA
Email: husainmohddanish@gmail.com
Vijay Vishnu Wagh The genus Aconitum L. (Ranunculaceae)
Plant Diversity, Systematics and Herbarium Division, CSIR characterized by hooded zygomorphic flowers with
–National Botanical Research Institute, Lucknow-226001, hidden nectaries and characteristic seed-coat
Uttar Pradesh, INDIA, Email: vijaywagh65@gmail.com ornamentation comprises of ca. 300 species worldwide
and is represented by 27 species and 5 varieties from
Tribal community of Jhabua district uses the forest
India. This highly medicinal genus is confined to
resources especially plants primarily for curing various
Himalayan region and taxa are well represented in both
ailments. Ethnobotanical practice has prevailed in this
western and eastern Himalaya. While examining the
area since the ancient time and the invasive plants are
herbarium vouchers from The Natural History Museum,
not the exception. Exhaustive field surveys were
London (BM) during the revisionary study on the tribe
conducted for the collection of the ethnobotanical data
Delphineae, we came across a specimen of Aconitum
and voucher specimen. Information regarding
sp. collected by F. Kingdon-Ward (Coll. No. 20246)
ethnobotanical uses of plants was collected from the
in 1950from Walong, Lohit Valley, Assam (now
tribals using a semi-structured questionnaire. Several
Arunachal Pradesh) at an altitude of 10,000 ft. asl.
extensive reviews which studied invasive plant species
This herbarium voucher has been identified and
are available. From this survey, a total of 102 plant
annotated as Aconitum duclouxii H. Lév. by Y.
species belonging to 38 families were reported for
Kadotain the year 2000. After more closer studies and
curing 37 types of ailments. Asteraceae was the
critical examination of the specimen, we could conclude
dominant family and in life form category herbs stand
that the annotation by Kadota is agreeable and literature
dominant. Leaf is the most frequently used plant part,
surveys confirm that the taxon has never been reported
where as decoctionis the highly preferred preparatory
from Indian Himalayan Region, thus, confirming a new
method for medicine preparation in the study area. 56%
record for Indian flora. Previously, the taxon was
of the invasive plants showed a use value of more than
known endemic to Yunnan, China and this study show
the extended distribution of the taxon in Arunachal series of benefits to the local, regional, and national
Pradesh which is adjacent to Yunnan region, thus making populations and include raw materials, patrimony
the new report from India. A. duclouxiiis closely allied protection, and scenic beauty.
to A. nagarum Stapf and both of them belong to Key words: Fundamental, Nutrition, Medicines, Fuel,
Aconitum subgen. Aconitum, a group of biennials with Physicians, Protection.
paired tuberous roots, but differs from the latter in
having pedicels with spreading glandular hairs, SII/O-21
bracteoles broader, carpels with hirsute golden hairs,
petals with a comparatively shorter lip, and petal-lip Documentation of Pollinator Floral
with inconspicuous veins and entire, non wavymargins. Calendar: A Case Study from Kashmir
Key words: Aconitum, Assam, Ranunculaceae. Himalayas
SII/O-20
Zubair A. Rather 1*, Anzar A. Khuroo 1, Shoukat
Role of the Plants Used in the Ara2, Rameez Ahmad1 and Maroof Hamid1
1
Centre for Biodiversity and Taxonomy, Department of
Traditional Medicine Botany, University of Kashmir, Srinagar-190006, J&K, INDIA;
2
Research and Training Centre for Pollinators, Pollinizers
Mukul Kumar and Pollination Management, Sher-e-Kashmir University of
Department of Botany and Plant Physiology, M.B.A.C., Agricultural Sciences and Technology, Srinagar-190025, J&K,
Agwanpur, Saharsa, (B.A.U. Sabour), Bihar, INDIA INDIA, Email: zubairrather4@gmail.com
Email: drmukul.bau@gmail.com
Insect pollinators play a vital role in the pollination
Plants have played a fundamental role for the of plants occurring in natural as well as human-
development of cultures ever since man first arrived dominated ecosystems. Thus the availability of
to that region approximately 10000 years ago. Through sufficient forage resources, pollen and nectar,
history, man has utilized vegetable resources as a source throughout the calendar year is the pre-requisite for
of nutrition, medicines, fuel, and building materials and the sustainable population of pollinators, and the
they even occupied an important place within their productivity of pollinator-dependent ecosystems. The
system and rites. The use of plants for treating diseases present study documented the insect pollinator and
keeps being necessary Tokoshi zone in Bihar, which, flower interactions in different seasons during the
due to high costs, have a difficult access to western years2012-2016 in Kashmir Himalaya. Based on
medicines and, therefore, use to really on their own systematic surveys and field observations, at each site,
traditional remedies. On the other hand, the integrated interactions were recorded at five points along 30m
forms of modern and traditional medicines are often trail by moving in parallel directions for 60 minutes.
practiced by many physicians nowadays. This has given Flowering period for a particular pollinator foraging plant
origin to a popular medicine comprising the use of more species was observed from the date of opening of first
than 100 local plants, which have been botanically flower to the date of shedding of last flower. Our results
identified. The illnesses cured with the plants are of suggested that majority of forage resources are
course, strictly related with the health situation of local available in the month of May (84%) followed by June
communities. Wound healing aids, renal and stomach (70%), July (53%), April (46%), whereas the insect
infections, headaches, and colds are the most common pollinators especially managed honey bees suffer for
ailments which natural resources are used for. forage resources in the winter months of December,
Nonetheless less frequent diseases, such as cancer, January and February during which only 3.77%, 4.71%,
diabetes, and malaria, are also treated with different 8% forage resources are available respectively. The
plant extracts. According to the World Health present study provides pollinator floral calendar of
Organization about 80% of the world population uses frequently visited forage plants and the period of their
natural remedies and traditional medicine. Such availability for the pollinators in different seasons across
medicine comes from plant resources that provide a the year. The results from the present study will help
in better management of honey bees in apiculture and resulted in maintenance of a thick Cedrusdeodara
also continuous availability of pollinators for sustainable forest from which no resources are extracted except
productivity in agriculture and horticulture in this in emergencies. For water, setting up of baudies
Himalayan region. (water reservoirs) and devtaals (sacred ponds) such
Key words: Pollinators, Pollination, Floral calendar, as Dynasur are pertinent examples. Polluting them is
Himalayas, Agriculture. a sin. For soils, terraces and bund formation are
important practices. Ghasnis- for grassland protection
SII/P-1 ensures sustainable availability of fodder during lean
season. In a nut shell, besides using biodiversity, the
Traditional Uses of Biodiversity Bhangalis are also involved in practices that guide its
amongst the Bhangalis and the conservation. Unfortunately, these practices are
breaking under the influence of market forces and
Practices for their Conservation changing lifestyle. Thus, strategies for their
documentation, preservation and maintenance become
Alpy Sharma1 *, Sanjay Kr. Uniyal2 and Daizy R. important.
Batish3
Key words: Bhangalis, Biodiversity, Conservation,
1
High Altitude Biology Division, CSIR-Institute of Himalayan Management, Market.
Bioresource Technology, Palampur-176061, Himachal
Pradesh, INDIA; 2High Altitude Biology Division, CSIR- SII/P-2
Institute of Himalayan Bioresource Technology, Palampur,
HP 176061 INDIA; 3Department of Botany, Panjab University, Poplar and Other Fast-Growing Tree
Chandigarh-160014, INDIA, Email: sharmaalpy@gmail.com,
suniyal@ihbt.res.in, daizybatish@yahoo.com Culture Outside Forest
Recognizing the importance of biodiversity and Anita Tomar*, Anubha Srivastav and Abhijeet
its conservation, the present study was conducted to Pandey
document traditional uses of biodiversity and various
conservation practices followed by the Bhangalis- Forest Research Centre for Eco-rehabilitation, 3/1, Lajpat
residents of Chhota Bhangal. The Chhota Bhangal lies Rai Road, New Katra, Allahabad, Uttar Pradesh, INDIA
Email: anitatomar@rediffmail.com
in the lap of Dhauladhar mountain range, Himachal
Pradesh. Field surveys were carried out to the area Plantation forestry outside the forest in India is
and personal interactions were held with the Bhangalis largely based on fast-growing trees. This is particularly
to record use of wild plants for edible purposes. The true for agroforestry which is practised by individual
study also targeted documentation of plants derived growers in their farmland and wasteland. Trees are
household products and the traditional conservation systematically planted in farmland as block plantation
practices prevalent in the area. The results revealed or boundary plantation, or maintained as scattered trees
use of 50 wild plant species for edible purposes. for various tangible and intangible benefits. The Forest
Maximum of these were used as vegetable (mean=8.9) Survey of India has estimated the growing stock of
while the minimum was for brew (mean= 0.4). trees outside forest at 1573 billion cubic metre (27.3%
Bhangalis used 55 plants derived products of which of total growing stock). However, the annual wood
majority were used as indigenous tools (34). For making production of trees outside forest is about 13 times more
these products, 20 species belonging to the 12 families than the forest, suggesting the popularity of fast-
were used. Maximum of these belong to the family growing trees outside the forest. The boost to tree
Pinaceae and 50% of used species were trees. As planting outside the forest was provided by the National
evident, since their livelihood is dependent on Commission on Agriculture, 1976 and the National
bioresources, Bhangalis ensure their flow and Forest Policy, 1988. Eucalyptus, Casuarina, Populus,
availability by following traditional practices for forest, Dalbergia, Tectona, Melia etc. are among the most
soil, water, and grassland conservation. Presence of a popular plantation trees. Despite this, several policy
sacred grove dedicated to local deity Ajayapaal has interventions are adversely affecting the interests of
tree growers. Difficulties in obtaining felling and transit weight, lenticels, days of harvest and fruit per plants)
permits, absence of a favourable minimum support price and information on vegetative as well reproductive
mechanism, non-availability of soft bank loans, absence (Leaf, flowers, inflorescence and fruits) parts of the
of agroforestry cooperatives, Uncertainty about Sechium were studied for its morphological characters.
marketing/ sale of end produce, Small landholding, Poor From the investigation, the collected germplasms of
technical knowhow regarding planting practices and the fruits shows great variation in its appearances
suitable tree crop combinations, inadequate availability (shapes, colour, spines distribution) and weight. The
of superior planting material, etc., pose serious plant was found to be herbaceous, monoecious,
challenge to the grower. Populus deltoides, in perennial, climber, supported by branched tendrils and
particular, witnesses large fluctuations in the price of leaves, with light to dark green fruits. Chromosome
wood which renders the planting rate of this species count are tried to establish correct chromosome number
highly variable from year to year. A low level of in all the 14 genotypes as well as ploidy level and
technical knowledge about tree cultivation too deviations, if any. Since the distribution of the species
sometimes results in losses to growers. Insufficient is restricted to a limited area across the world, it is
research grant is also proving a handicap in technical worthwhile to preserve and conserve the species.
innovations in plantation forestry. Aninitative was taken Key words: Sechium edule (Jacq.) Swartz, Phenotype,
by Forest Research Centre for Ecorehabilitation (A Genotype, Chromosome number, Genetic diversity.
Centre of Forest Research Institute, Dehradun) for
planting fast growing tree outside forest in which Poplar SII/P-4
deltoides, Eucalyptus, Tectona grandis, Gmelina
arborea agroforestry trial are proposed in Gorakhpur, Diversity of Parasitic Algae Cephaleuros
Basti, Varanasi, Balia and Allahabad districts. Species (Trentepohliaceae, Chlorophyta)
Key words: Trees outside forest, Challenges, Policy, Poplar, In Kerala, India
Plantation forestry.
important plants. During the present study investigation IAA+BAP+AC combination proved optimal for early
on bryophytes collected from Dzukou valley, Saramati rhizogenesis and seedling development. The
Hills and Thanamir have been carried out. It revealed morphogenetic response of pseudobulb explants were
the occurrence of 99 species of mosses belonging to varied depending upon in vivo grown plants and in
52 genera of 26 families. Among the investigated vitro raised plantlets. In vivo explants induced
families in mosses, Bryaceae, Dicranaceae and regeneration response via direct PLB formation at BAP
Pottiaceae seem to be more dominant in the region, 3 mg l-1. Interestingly, TDZ (3 mg l-1) supplemented
followed by Meteoriaceae and Polytrichaceae. Genus with AC (2 g l-1) and IAA (2 mg l-1) + TDZ (3 mg l-1)
Pogonatum and Pohlia have maximum 6 number of + AC (2 g l-1) proved optimal for regeneration, culture
species followed by Genus Bryum and Dicranum with multiplication and healthy plantlet formation in the in
4 species. Atrichum crispulum Schimp ex Besch. has vivo and in vitro explants respectively. Synthetic seed
been recorded as new to India, while 54 species of technology is today avital tool for the in vitro
mosses have been reported for the first time from propagation and conservation of plants. Protocorm like
Nagaland. bodies (PLBs) formed in vitro were encapsulated in
Key words: Nagaland, Moss, North-East India. 3% sodium alginate matrix to form synseeds.
Encapsulation ensures slow growth and long term
SII/P-10 preservation. The effect of storage at 4oC and 25oC
was tested on the conversion frequency of seeds at 15
In vitro Seed Germination, Regeneration days intervals. Their multiplicity and conversion
frequency was markedly impaired upon storage. The
and Synthetic Seed Production Based protocol developed in the present investigation can be
Micropropagation of a Terrestrial commercially exploited and used as a means for the
Medicinal Orchid (Malaxis acuminata rapid propagation and conservation.
D. Don) Key words: Pseudobulb, Mitra medium, Micropropagation,
Protocorm like bodies, Orchid.
Anuprabha1* and Promila Pathak2
SII/P-11
1
GGDSD College, Sector-32, Chandigarh-160032, INDIA;
2
Orchid Laboratory, Department of Botany, Panjab A Preliminary Study on the Relative
University, Chandigarh-160014, INDIA, Email:
anu857pu@gmail.com, ppathak2007@gmail.com Abundance and Diversity of Insect
Malaxis acuminata is a rare, terrestrial, Pollinators in Different Fruit Orchards
pseudobulbous, shade loving, and medicinal orchid of of Kumaun Himalaya, India
Himalayan region. It is used as aphrodisiac, febrifuge
and has a cooling effect. Commercially known as Fasuil Farooq* and Manoj Kumar Arya
‘Jeevak’, its dried pseudobulbs from a constituent of Insect Biodiversity Laboratory, Department of Zoology, DSB
‘Ashtavarga’ drugs used in the preparation of ayurvedic Campus, Kumaun University, Nainital-263002, Uttarakhand,
tonic ‘Chyavanprash’. Its natural populations are INDIA, Email: faisalmalik.jk@gmail.com
decreasing mainly due to habitat destruction and
commercial collection pressures, so several efforts have Pollinators are regarded as important keys for
been made to develop an efficient micropropagation the production of fruits to increase farmers income.
system. Presently the immature seeds (6, 10, 14 wap) The present study aims to study the relative abundance
were inoculated on M medium with plant growth and diversity of insect pollinators in three fruit orchards,
regulators [IAA (1 mg l-1) and BAP (1 mg l-1)], organic i.e. apple, peach and apricot orchards, respectively.
supplements [YE (2 g l -1 )] and AC (2 g l -1 ). Scan sampling method was used to explore the diversity
Combination containing IAA supplemented with AC of insect pollinators. Observations of the insect
proved optimal for early germination and rapid pollinators were conducted from 8.00 to 11.00 AM and
protocorm multiplication; media containing 2.00 to 4.00 PM in sunny days. A total of 563 individuals
were collected, belong to 47 species in four order as one of the biodiversity hotspots. Himalayan
(Hymenoptera, Diptera, Lepidoptera and Coleoptera). grasslands have been classified into five types viz.,
Number of species of insect pollinators found in apple warm temperate grasslands, cool temperate grassy
orchard (31 species) and peach orchard (22 species) slopes, sub alpine meadows and thatches, alpine
were higher than that in apricot orchard (13 species). meadows and steppe formations of the trans-
Insect pollinators in all the three fruit orchards were Himalayas. Grasses have significance in human life
dominated by giant honey bee (Apis dorsata) followed far beyond meeting the basic requirements of food and
Apis cerana and Bombus haermorrhoidalis. Higher fodder. Economic uses include their use as raw material
foraging activities of insect pollinators occurred in the of sugar and beverages (the sugarcane), as constituent
morning. of baking powder, pies and puddings, building materials
Key words: Abundance, Diversity, Fruits, Insects, Species. for huts and tree homes (machons), art and craft,
vaulting poles (bamboos and reed grasses), paper,
SII/P-12 sweeping brooms (broom grasses) and finishing and
stiffening material in textiles (corn starch). Several
Ecology and Economy of Grasses of grasses are sown as lead plants to reclaim the unused
lands like mud flats and tidal estuaries (eg. cord grass)
Trans-Himalayan Region and alkaline and saline lands. The present study reports
the ecological and economic importance of grasses of
Mudassir Ahmad Bhat*, Priya Badgal, Poonam
Trans-Himalayan region. The grasses for which data
Chowdhary and Amarjit Singh Soodan
on the economic and ecological significance has been
Department of Botanical and Environmental Sciences, Guru presented in the paper include Piptatherum gracile
Nanak Dev University, Amritsar-143005, Punjab, INDIA Mez, Piptatherum munroi (Stapf) Mez, Stipa brandisii
Email: mabhat90@gmail.com Mez, Brachypodium sylvaticum (Huds.) P. Beauv.,
Poaceae constitutes the fifth largest angiosperm Koeleria macrantha (Ledeb.) Schult., Trisetum
family as far as the number of species is concerned. aeneum (Hook.f.) R. R. Stewart, Agrostispilosula
Within monocotyledons, the family stands at the second Trin., Calamagrostis holciformis Jaub. & Spach.,
rank after Orchidaceae and includes more than 11000 Calamagrostis pseudophragmites(Haller f.) Koeler,
grass species distributed in 700-800 genera. India is a Muhlenbergia huegelii Trin, Deschampsia
megabiodiversity country that harbours a rich diversity cespitosa(L.) P. Beauv., Dactylis glomerata L.,
of grasses. Poaceae has emerged as the largest plant Poaalpina L. Eremopoaaltaica (Trin.) Rozhev.,
family in the country with a representation of 268 genera Alopecurus himalaicus Hook. f., etc. and
and 1300 species. Ever since their origin and economically important include Koeleria litvinowii
diversification, grasses have evolved a number of biotic Domin, Trisetum spicatum (L.) K. Richt., Festuca
and abiotic interactions that have made them an pratensis Huds., Lolium perenne L., Poa albertii
important component in almost all ecosystems but Regel, Poa pratensis L., Alopecurus arundinaceus
dominant elements in the grasslands of the world. Apart Poir., Phleum alpinum L., Bromus catharticusVahl,
from monitoring, grasses have been found to possess Bromus inermis Leyss, Elymus nutans Griseb., Elymus
tremendous potential to maintain the carbon balance dahuricus Turcz. Ex Griseb., Bromus japonicas
and mitigating the damage to the environment. Several Houtt., Hordeum brevisubulatum (Trin.) Link,
grass species have been identified and recommended Pennisetum orientale Rich., Setaria italic (L.) P.
as bioenergy crops to obtain bio-fuels that would reduce Beauv. and Tenaxia cumminsii (Hook. f.) N.P. Barker
the burning of fossil fuels and release of Green House & H.P. Linder.
Gases (GHG’s) into the atmosphere. Within the country, Key worlds: Poaceae, Grassland, Himalayas.
north western Himalayan region has been recognized
the moist tropics. The present study revealed 45 species (H’=3.325) and large (H’=3.185) homegardens. Tree
under 12 genera in Caesalpinioideae from Uttar density was found to be higher in the large homegardens
Pradesh, including 13 cultivated species. Among (249 trees ha-1) and followed by medium (216 trees
different genera, Senna is the dominant ones with 12 ha-1) and small homegardens (195 trees ha-1). The study
species which constitutes about 26% of total shows that vegetables are the major component of the
Caesalpinioideae occurring in Uttar Pradesh. The homegardens followed by fruit, medicinal, firewood and
genera Bauhinia and Caesalpinia hold second and ornamental plants. The households recorded most
third position with 9 (20%) and 6 (13%) species species as useful for vegetable (24%) followed by fruit
respectively in the state. Out of 45 species, 10 species (18%), firewood (12%), medicine (11%), ornamental
are commonly occurring throughout the state in (9.8%), fodder (9.2%), timber (6.7%) and others
different biogeographic regions like Terai Region, (9.3%). Higher values of biomass were recorded in
Gangetic Plain, Vindhyan Region and Semiarid Region. small followed by medium and large homegardens, and
While, some of the species are area specific, that occurs thus biomass carbon stock amongst the homegardens
only in very restricted pockets. Each species has been was in the order: small (122.3 Mg C ha-1) > medium
dealt with detailed taxonomic information such as (109.0 Mg C ha-1) > large (90.6 Mg C ha-1). Soil organic
correct nomenclature, description, distribution, carbon (SOC) stock upto 1 m soil depth was also
phenology and line drawing illustrations or photographs. recorded maximum in small (229.5 Mg C ha-1) followed
Key words: Caesalpinioideae, Diversity, Uttar Pradesh, by medium (176.2 Mg C ha-1) and large (144.6 Mg C
Taxonomy. ha -1 ) homegardens. The study results indicate that
traditional homegarden agroforestry systems mimics
SII/P-19 forest ecosystems and thus qualify as potential carbon
sinks through sustainable management practices.
Biodiversity Conservation and Ecosystem Key words: Homegardens, Species diversity, Biomass carbon
Carbon Storage in Traditional stock, SOC stock
Homegardens in Mizoram, Northeast SII/P-20
India
Algal Diversity with Special Reference
Soibam Lanabir Singh and Uttam Kumar Sahoo* to Chlorophyceae of Shershah Suri Pond,
Department of Forestry, School of Earth Sciences and Natural Kanpur, U.P.
Resource Management, Mizoram University, Aizawl-796004,
Mizoram, INDIA, Email: uksahoo_2003@rediffmail.com,
Vishal Saxena* and R.K. Khare
uttams64@gmail.com
Department of Botany, Govt. Model Science College (Jiwaji
The study was conducted to document species University), Gwalior, M.P., INDIA, Email:vishsaxena@
composition, their utilization and carbon storage in rediffmail.com, rakesh.khare1965@ gmail.com
traditionally practiced homegardens in Mizoram,
Northeast India. A stratified sample of 42 homegardens Fresh water is natural source of fundamental
classified on the basis of size as small (0.02 to 0.05 importance to all living beings on earth. Natural water
ha), medium (0.05 to 0.75 ha) and large (> 0.75 ha) is rich in bacteria, algae, protozoa, worms and other
with 14 homegardens each were surveyed in 2017-18. organisms. Algae, carbon fixing and oxygenating
A total of 198 species (82 trees, 31 shrubs and 79 herbs) organism, and their diversity is the key parameter to
belonging to 69 families and 169 genera were recorded, determine the productive nature of the pond ecosystem.
being most of them perennials (78 %). Overall highest The limnological survey and the algal diversity of
plant species was recorded in small (157 species) Shershah Suri freshwater pond in Kanpur (26o 24’29"
followed by medium (141 species) and large (141 N latitude and 80o 23’34’E longitude) district, Kanpur
species) homegardens. Diversity index for trees was Nagar (U.P.) was undertaken. Water samples were
maximum in medium (H’=3.780) followed by small analysed for the concentration of various physico-
chemical parameters. Phytoplankton of all the sides of
pond were collected for analysis. Detailed examination of Selinum also fall under the Catalogue of Life list by
shows that algae exhibit a high degree of qualitative Catalogue of Life Indexing. Since the plant has
and quantitative variance throughout stretch of principal constituents (alkaloids as well as essential oils)
Shershah Suri Pond. The aquatic system have shown mainly in the root, the plant is completely destroyed
existences of 34 algal forms and out of them 12 belongs while being put to use. To avoid mass destruction of
cyanobacteria, 4 to Euglenophyceae, 7 to this high altitude medicinal plant, several conservation
Bacillariophyceae and 11 to chlorophyceae. There are strategies need to be under taken such as cultivation,
certain algae occur in all the season. The present paper germplasm conservation, seed bank procurement etc.
deals with the study of members of chlorophyceae e.g. Conservation of plants can be tackled by scientific
Cladophora, Oedogonium, Spirogyra, Vaucheria and techniques such as in situ conservation to secure
Zygnema. genetic variation and also conserve population of
Key words: Algal diversity, Chlorophyceae, Aquatic system, Selinum; ex situ conservation or biodiversity
Shershah Suri Pond. management for human utilization; local action such
as Legislation, to give sustainable benefit to the present
SII/P-21 generation while retaining its potential to meet the
requirements and aspirations of future generations as
Conservation Strategies in Selinum: well.
A Himalayan Medicinal and Aromatic Key words: Conservation, Threatened, Medicinal, Bhutkeshi,
Selinum, Apiaceae.
Herb
SII/P-22
Ravi Prakash Srivastava* and Gauri Saxena
Department of Botany, University of Lucknow, Lucknow- A Preliminary Diversity Analysis of
226007, INDIA, Email: ravisrivastava02@gamil.com, Dicotyledonous Plants of Dima Hasao
gaurigupta72@ yahoo.com
District of Assam, India
Medicinal plants and herbal medicines form an
important part of the treatment in the Indian medicine R.K. Verma1*, B.A. Khan1, V.V. Wagh1, Baleshwar1,
systems. One such Himalayan medicinal herb is S.K. Behera1, S. Tamta2 and L.B. Chaudhary1
Selinum that belongs to Apiaceae family. The plants
1
are also widely distributed over the temperate zones Plant Diversity, Systematics and Herbarium Division, CSIR-
of both the hemispheres. The genus commonly referred National Botanical Research Institute, Lucknow-226001,
INDIA; 2Department of Botany, DSB Campus Kumaun
to as Bhutkeshi in India, is known for its medicinal and
University, Nainital-263001, Uttarakhand, INDIA, Email:
aromatic properties and has been used as an alternative dr_lbchaudhary@rediffmail.com
source and adulterant of compounds obtained from
Nardostachys jatamansi. All the plant parts including The study enumerates the dicotyledonous plants
leaf, stem and root are utilized for medicinal uses such found in the district Dima Hasao in Assam with their
as nervine sedative in hypertension, aphrodisiac with important taxonomic information. This is the outcome
analgesic properties and in common ailments like cold, of the several botanical tours conducted to different
cough, fever, wounds, stomachache and toothache etc. parts of the district during 2017–2018. Dima Hasao
It is also used for aromatic purposes such as incense. district, formerly known as North Cachar Hills district,
These plants have been locally as well as globally is located in the southern part of Assam between 240
exploited that is why the current status of plant is now 582 to 25o472 N latitudes and 92o272 to 93o432 E
near threatened. According to the IUCN Red List 2001, longitudes at elevations ranging from 140 to 1866 m.
the species Selinum carvifolia, was designated as The entire district comprises about 89 % forest cover
endangered in the United Kingdom. Furthermore, of its total geographical area of 4,888 sq. km. with
another Red List by the IUCN in 2009 indicated evergreen, semi-evergreen and deciduous types of
Selinum vaginatum as threatened species and was forest. The Jhoom cultivation is the major agricultural
considered to be in low risk category. Several species practice in the area while the settled agricultural land
is only 1.29 % of the total geographical area of the pharmacological aspects, ecology, growth condition and
district. After conducting several tours, about 436 environment needed; products and by-products,
species belonging to 307 genera and 87 families have international and local market and current scenario
so far been collected and identified from the study area. about demand and supply, current issues and challenges
All voucher specimens have been deposited in CSIR- for conservation, domestication and commercial
National Botanical Research Institute, Lucknow cultivation, future prospect and recommendations and
(LWG). Based on life forms 184 species have been need of policy support etc.
identified in herbs, 70 in shrubs, 146 species in trees Key words: Pedalium murex, Bada Gokhru, Large Caltrops,
and 39 species in climbers. Among all families, Medicinal plant.
Fabaceae with 92 species, and among genera,
Crotalaria with 9 species were found largest in the SII/P-24
study area. Some of the other dominant families are
Asteraceae (34 spp.), Euphorbiaceae (21 spp.), Checklist of Green Algae (Chlorophyceae)
Amaranthaceae (20 spp.), Malvaceae (14 spp.), of Uttar Pradesh, India
Acanthaceae (12 spp.) and Convolvulaceae (10 spp.)
Similarly, Ficus (8 spp.), Desmodium (6 spp.) and Sushma Verma* Kiran Toppo and Sanjeeva Nayaka
Senna (5 spp.) are frequently occurring genera. One
of the interesting plants Nepenthes khasiana has also Algology Laboratory, CSIR-National Botanical Research
Institute, Lucknow-226001, INDIA, Email: creation.sanju@
been relocated from the area. During the present study,
gmail.com
about 47 highly medicinally important and useful plants
were also recorded from the study area based on the Algae are predominantly aquatic photosynthetic
information gathered by locals. organisms and are the major primary producers of
freshwater ecosystem. The class chlorophyceae are
Key words: Diversity, Dima Hasao District, Dicotyledon,
Assam. diverse group of green algae comprising of various
forms distributed widely in freshwater bodies and
SII/P-23 terrestrial habitats. They include some of the most
common species as well as several members that are
Pedalium murex L. from Wild to ecologically significant. Thallus organization of this
group varies from microscopic unicellular to
CommercialProduction:An Understanding
multicellular macroscopic forms, may be colonial,
with Issues and Challenges coccoid, palmelloid, sarcinoid, syphonacious,
filamentous, thalloid and or pseudoparenchymatus. In
Sujata Shekhar*, Akhilesh Kumar and J.N. India the total algal taxa are 7396, with class
Shrivastava chlorophyceae representing approximately 2592 taxa.
Department of Botany, Dayalbagh Educational Institute The present checklist of class chlorophyceae is the
(Deemed University), Agra-282005, Uttar Pradesh, INDIA first compilation of the freshwater algal flora of Uttar
Email: akhilpbh@rediffmail.com Pradesh based on the updated literature records and
Pedalium murex (Pedaliaceae), commonly bibliographic data intending to contribute towards the
known as Large Caltrops in English and Bada Gokharu knowledge and information on the algal biodiversity of
in Hindi. It is a mucilaginous medicinal herb having the state. The list summarizes the bibliographic database
multiple uses in traditional medicine system. It is mainly from approximately 120 scientific research papers, 6
used to cure reproductive disorders, like impotency in books and 20 theses from libraries of various Research
men, nocturnal emissions, gonorrhea as well as Institutes and Universities in Uttar Pradesh. The
leucorrhoea in women. It is also useful in the treatment taxonomic identity of the taxa is checked by
of urinary and gastrointestinal tract disorders. The www.algaebase.org designed by M. Guiry. The present
present paper deals with botanical description, origin, list records 973 taxa of class chlorophyceae pertaining
diffusion and distribution, usages in traditional and to 9 orders, 35 families and 115 genera from 24
modern system of medicine, phytochemical and different aquatic and terrestrial habitats of
approximately 65 districts of Uttar Pradesh. The genus reticulate, foveolate, reticulately-rugulate, reticulately-
Cosmarium was recorded to be the dominant taxa with foveolate, stellate papilllate, irregularly thickened cell
approximately 142 species followed by the genus walls, irregularly ribbed to faintly tuberculate. Although,
Oedogonium with approximately 100 species. The these characters may not be utilized to classify the
least number of taxa recorded are from the genus genus at sungeneric or sectional, but definitely they
Papenfussiomonas, Trochiscia, Sphaeroplea and add an additional features to the individual taxon, which
Protosiphon represented with single species each. The tremendously help in the identification.
present study could be considered as a baseline data Key words: Diversity, Seeds, SEM, Identification, Astragalus.
for further exploration on the algal biodiversity from
the state of Uttar Pradesh. SII/P-26
Key words: Green algae, Freshwater Chlorophyceae,
Checklist, Uttar Pradesh Distribution and Diversity of Lichens
in Dima Hasao district, Assam with
SII/P-25
Special Reference to Forest Health
Application of Macro and Micromor-
Gaurav K. Mishra, Sanjeeva Nayaka and Dalip
phological Characters of Seeds in Kumar Upreti
Diversity Analysis of Astragalus L. Lichenology Laboratory, CSIR-National Botanical Research
(Legumonosae) in India Institute, Rana Pratap Marg, Lucknow-226001, U.P., INDIA,
Email: gmishrak@gmail.com
Shivani Kashyap and L.B. Chaudhary The Indian Himalayan region exhibit rich diversity
Plant Diversity, Systematics and herbarium Division, CSIR- of lichens in India. The Western Himalayan region has
national Botanical Resarch Institute, Lucknow-226001, luxuriance of lichens due to its unique topography and
INDIA, Email: dr_lbchaudhary@rediffmail.com climatic condition, however, the Eastern Himalayan
Astragalus L. is the largest angiospermic genus region showed rich diversity of lichens due to its warmer
in the world with about 2900 species. In India the genus and moist climate condition. The rich moist and warmer
which is chiefly found in the Himalaya, is represented climate in Eastern Himalayan region supports forest
by about 79 species. Due to its large size and enormous with dominance of evergreen tree vegetation. The
morphological plasticity, the taxonomy of the genus is Dima Hasao district of Assam is situated at higher
very complex and challenging. The identification of altitudes between 500 to1500 m having a suitable
species only based on macro morphological characters condition for many lichen taxa belonging to family
is tedious in the genus. In the present study, therefore, Graphidaceae, Pertusariaceae, Pyrenulaceaeand
both macro and micro morphological features of seeds Thelotremataceaeas the micro lichens together with
like shape, size, colour and spermoderm pattern under macro-lichens families such as Parmeliaceae,
Scanning Electron Microscope (SEM) have been Physciaceae, Collomataceae and Cladoniaceae. The
examined to evaluate their role in the identification and district is represented by occurrence of 125 species
classification. The seeds of 30 species belonging to belonging to 54 genera and 16 families. Among the
about 14 sections have been analysed for the study of different genera of lichens, Graphis showed its
variations among them in the present study. The overall maximum diversity represented by 20 species followed
size of the seeds ranges from 1.5-3.2 x 0.8-2.2 mm. by lichen genera Parmotrema and Pyrenula with 10
The shape of the seeds, observed in the investigated and 9 species respectively. The study area has
species, are reniform, ovoid, oblong, discoid, dominance of crustose lichens represented by 91
suborbicular and deltoid. In the majority of the cases species followed by 27 species of foliose lichens. Based
the seeds are brown or blackis-brown. The on the occurrence of lichen bio-indictor communities
spermoderm pattern showed great variability in the study area can be mapped in to different zones
ornamentations which exhibit variation from rugulate, having different levels of environmental conditions and
forest health. The Lobariantaxa considered as sensitive
to micro climatic condition and air quality are reliable well as Bacteria. Fungi isolated were mostly from the
indicators of species rich old forest with long forest group Basidiomycetes, Ascomycetes, Deuteromycetes
continuity. The rich diversity of all the growth forms of and Phycomycetes. Bacterial isolates include
lichens clearly indicates that moist of the forest within Staphylococcus sp., Streptococcus sp., Micrococcus
the district of Dima Hasao has good health. The forest sp., Pseudomonas sp. The Bacterial identification was
in Ethenic village area of Halflong and Umranso are done with reference to Bergey’s Manual of
considered as forest having good health and rich old Determinative Bacteriology and that of fungus by Book
forest having ecological continuity. The fast pace of for Fungal Identification. The results and comparative
urbanization and other developmental activities within study revealed that both the ponds are highly polluted
the district will definitely have adverse effects on the with diverse microorganisms, some are harmful for
diversity and distribution of lichens in different forest health and the water not safe for the domestic use.
type and the present distribution pattern will act as base There has been great impact on physicochemical
line data for carrying out future bio-monitoring studies parameter like DO, BOD, COD that affects the every
in the area. component of ecosystems. The details and effect of
Key words: Biodiversity, Eastern Himalaya, Climate change, seasons are discussed in detail in paper.
Bio-monitoring. Key words: Microbiota, Biodiversity, Bacteria, Fungi, Water.
SII/P-27 SII/P-28
from August, 2015 to January 2017, which shows the from 36.3 to 201.8 Mgha”1 . Total C storage ranged
occurrence and distribution of the aquatic macrophytes between 16.3 and 92.8 Mgha”1. The range of Shannon–
in the selected ponds, and were classified according to Wiener diversity index was between 0.31 and
their habit. Both the ponds are filled with water all the 1.08.Furthermore most of the conifer-dominated forest
year round, its vegetation which includes 53 different types had higher C accumulation potential than
species of aquatic macrophytes were found from the broadleaf-dominated forest types. Protection of the
two ponds , species distribution and seasonal variation conifer-dominated forest stands from deforestation
are discussed in paper. 36 Algal genera were recorded especially Cedrus deodara, Abies pindrow, Pinus
from the class Chlorophyceae, Cyanophyceae and wallichiana and Pinus roxburghii would have the
Bacillariophyceae. Oscillatoria was found throughout largest per-unit-area-impact on reducing human-
the year. Chlorophycean microphytes dominated during induced C emission. The results also showed that the
the monsoon season and Cyanophycean microphytcs forest biomass is a multipart property that integrates
after monsoon. Bacillariophycean microphytes have no diverse ecological functional and structural attributes,
relation with the season. The high algal population could thereby linking growing stock volume density(GSVD),
be due to equal exposure of aquatic surface to sun and basal area, height, and wood density and hence plays a
pH. significant role in C accumulation, but not the tree
Key words: Macrophytes, Microphytes, Diversity, diversity. The results of the present study help in filling
Phytoremediation, Aquatic ecosystem. the knowledge gaps in scientific studies on the forest
ecosystems in this eco-fragile part of the Himalayan
SII/P-29 region and hopefully would help in mitigating the regional
climate change by minimizing the anthropogenic
Carbon Accumulation Pattern in impacts within these forest ecosystems in the region.
Different Forest Types of Northwestern Key words: C accumulation, Diversity, Forest ecosystems,
Northwestern Himalaya.
Himalaya, India: the Role of Tree
Structural and Species Diversity SII/P-30
Shiekh Marifatul Haq1*, Maroof Hamid2, Anzar A. Assessment of Plant Growth and
Khuro2 and Irfan Rashid1 Physiological Performance among
1
Department of Botany, University of Kashmir Srinagar- Eight Varieties of Chickpeas in Indo-
190006, J&K, INDIA; 2Centre for Biodiversity & Taxonomy,
Department of Botany, University of Kashmir, Srinagar- Gangetic Plains of Uttar Pradesh
190006, J&K, INDIA, Email: snaryan17@gmail.com
Shiv Naresh Singh1*, Shruti Mishra1, N. Manika1,
Forests are considered as the important sinks for
Bilal A. Khan1, Nayan Sahu1, Nalini Pandey2 and
atmospheric carbon (C), yet C uptake and accumulation
Soumit K. Behera1
rates are highly uncertain, and the mechanisms remain
1
poorly understood. The aim of the study was to assess Plant Ecology and Climate Change Science Division, CSIR
the tree structural attributes such as (basal area and - National Botanical Research Institute, Lucknow-226001,
height), diversity, stem density, biomass and C stocks INDIA; 2Department of Botany, Lucknow University,
Lucknow-226007, INDIA, Email: 777shivlko@gmail.com
in different forests of Northwestern Himalaya and to
make recommendations for forest management and Chickpeas are the most popular pulse crop of
conservation based on priorities for biodiversity India, popularly known as Gram or Bengal gram, mainly
protection and C sequestration. Eleven forest types in grown in Rabi season. Chickpeas are a rich source of
sub-tropical to temperate zones (400-2900 masl) of the highly digestible dietary protein. There is as such no
region were studied during the study. The results of detailed study available on growth, biomass and yield
the present study revealed that stem density ranged of different varieties in Chickpeas, Cicer arietinum
between 140 and 357 Nha”1, while total biomass ranged L. with special reference to Indo-Gangetic plains (IGP)
of Uttar Pradesh. We compared the plant growth and loss caused by these species may soon surpass the
yield among 08 varieties of chickpea under farmer’s damage done by other anthropogenic activities.
field and screened the high yielding variety for Indo- According to IUCN, Alien Invasive Species are those
Gangetic region. Eight different varieties of Chickpeas alien species which become established in natural or
viz. HK94-134, JG -11, PANT-G -186, Radhey, semi-natural ecosystems or habitats, outside their
Avarodhi, Shubhra, Ujjwal and DCP-92-3 were natural distributional range and act as an agent of
analyzed in terms of growth, physiological attributes change causing threats to native biological diversity.
and yield in order to screen the best adaptive variety The opportunity of inadvertent introductions will may
with highest yield under rain fed conditions of IGP of become more with rapidly increasing global trade.
Uttar Pradesh. Field evaluation on the basis of During the course of floristic exploration of the Parvati
phenological traits, physiological performance, leaf area Aranga Wildlife Sanctuary and adjacent Tikri Forest
index and yield were carried out in randomized plots Area, Gonda district, Uttar Pradesh, the author has
with three replicates for each variety in Banda district recorded 381 species of flowering plants; out of these
of Uttar Pradesh. The measurements were made in 64 species belonging to 31 families analyzed as invasive
three growth stages i.e. vegetative phase, reproductive alien species. The species with American nativity
phase and senescence. We observed significant (Tropical America, Central America and South
differences in majority of evaluated traits among the America) were most dominant alien invasive, followed
08 varieties. Although, the morphological traits were by African, Asian, European and Mediterranean species
better in the variety ‘Ujjwal’ among all, but altogether, which hold the probability of their introduction through
‘Avarodhi’ observed the highest yield with maximum other agents along with the food grains, mainly by
photosynthesis rate. The above ground biomass was human beings. Further, the family Asteraceae
highest in Radhey variety with an average of dominated over all others with 09 taxa followed by
39.76±3.38 g plant-1 followed by Avarodhi (average Convolvulaceae, with 05 taxa, Euphorbiaceae and
30.33±2.64 g plant -1 ) and Shubhra. Highest total Papilionaceae with 04 taxa each. Invasive alien species
biomass was observed in Radhey (average 45.16±4.07 of the area and their impact on the wetland ecosystem
g plant-1) among all targeted varieties. From the present have been also discussed. A better planning is needed
findings, it may be concluded that Avarodhi has highest for early forecasting and reporting of infestation of
carbon sequestration potential with highest yield in the spread of these invasive weeds by creating plant
IGP of Uttar Pradesh in rain fed condition, which further detection network by collaborative links between
needs to be revalidated with more field trials at multiple taxonomist, ecologist and other stakeholders related to
locations. this field.
Key words: Chickpea, Indo-Gangetic plains, Uttar Pradesh. Key words: Alien species, Parvati Aranga Wildlife Sanctuary,
Tikri forest, Gonda.
SII/P-31
SII/P-32
Invasive Alien Flora of Parvati Aranga
Wildlife Sanctuary and Adjacent Tikri Aroma Yielding Grasses of India; A
Forest Area, Gonda District Uttar Potential Resource for Bioprospection
Pradesh, India Shailja Tripathi1,2*, Abhai K Srivastava1, Priyanka
Agnihotri1,2 and Tariq Hussain1,2
Vineet Singh 1
Plant Diversity, Systematics & Herbarium Division, CSIR-
Botanical Survey of India, Central regional Centre, National Botanical Research Institute, Rana Pratap Marg,
Allahabad-211002, Uttar Pradesh, INDIA, Email: Lucknow-226001, INDIA; 2Academy of Scientific and
vineet.singh332@gmail.com Innovative Research (AcSIR), Ghaziabad-201002, INDIA,
Email: tripathishailja16@gmail.com
Convention of Biological Diversity (1992)
visualizes that “biological invasion of alien species as Aromatic compounds have always been of great
the second worst threat after habitat destruction”. The significance due to a variety of chemical constituents
and have a diverse range of uses. Plant-based aroma an average of 0.40±0.17 alleles per loci. The neighbor
is of major choice for development of essential oil- joining (NJ) based clustering grouped all the accessions
based products whether it is pharmaceuticals, into three major clustering grouped were also confirmed
cosmeceuticals or perfumery industries. Moreover, by scatter plot of PCoA. Several potential accessions
nowadays aromatherapy has become the main line have been identified which could be of potential for
medication system for various health disorders. future T. cordifolia genetic improvement program.
However, despite the vast range of uses, extraction of Additionally, the grouping of various T. cordifolia
aromatic compounds has been limited to only a few accessions in three different clusters, as analyzed with
plant species resulting in the use of synthetic aromatic SSR markers, indicates that T. cordifolia has potential
compounds which in turn leads to the health side effects. inherent diversity that can be exploited towards genetic
Poaceae, the largest plant family in India comprising enhancement program.
1300 species belonging to 268 genera is of vital Key words: Tinospora cordifolia, microsatellite, genetic
significance useful in different economical aspects but diversity, NJ tree, PCoA.
its use is limited to only few species. In the present
paper, 14 grass species belonging to eight genera have SII/P-34
been documented for their aroma yielding properties.
Cymbopogon is found to be the most widely used grass Distribution and Current Status of
for aromatic properties, followed by Chrysopogon Thalictrum (Ranunculaceae) from
zizanioides, Eleusine indica, Eragrostis tremula, India
Thelopogon elegans, Sorghum arundinaceum,
Andropogon gayanus and Dicanthium sp. distributed Harsh Singh and Dibyendu Adhikari
in different parts of the country. The study is designed
with an idea of bioprospecting Indian grasses for their Department of Botany, North-Eastern Hill University,
aromatic and essential oil yielding properties on the basis Shillong-760002, Meghalaya, INDIA, Email: harshchamlegi@
gmail.com
of their traditional uses.
Key words: Aroma, Grasses, India, Bioprospection.
The genus Thalictrum L. (meadow rue) belongs
to the family Ranunculaceae and comprises 200 species
SII/P-33 mainly distributed in temperate regions of the world of
which twenty species with five varieties are found in
SSR Marker based Genetic Diversity India. The genus shows great complexities in its
morphological characters and is therefore a difficult
Analysis in Tinospora cordifolia and complicated genus for the identification. Throughout
the world, plants of this genus have been used as a
Suchita Lade 1,2 *, Hemant Kumar Yadav 1 and
general tonic, purgative, stimulant, aperient and for the
Veena Pande2
treatment of snake-bite, jaundice, rheumatism, wounds,
1
CSIR- National Botanical Research Institute, Lucknow- swellings, uterine tumours, paralysis, joints pain, nervous
226001, INDIA; 2Kumaun University, Nainital, INDIA, Email: disorders, toothache, diarrhea, piles, fever, peptic ulcers,
suchitalade.sl@gmail.com ophthalmic problems, headaches, as a tonic, diuretic,
The nature and extent of diversity in Tinospora etc. in all systems of medicine. Ecological niche
cordifolia, a dioecious climber, widely distributed in characterization and modeling potential distribution area
India, and one of the most important medicinal plants should help in developing strategies for its conservation.
has remained under explored. The present investigation In the present study, we used Maxent modeling to
aimed to explorethe level of genetic diversity in a larger characterize the environmental niche of the genus
set of germplasm of T. cordifolia using microsatellite Thalictrum and also assess the inter-specific niche
marker. A total of 96 accessions of T. cordifolia were differentiation. The finding of the study should be useful
evaluated for diversity analysis and SSRs markers in population survey and should be helpful in
developed for diversity assessment. A total of 77 alleles identification of sites for reintroduction and cultivation.
were amplified by 10 SSRs ranging from 4 to 12 with Key words: Thalictrum, Ranunculaceae, Maxent, Meadow rue.
SII/P-35 SII/P-36
Session III
Environmental Biotechnology
and Microbiology
SIII/KN-1 SIII/L-1
purpureum, Chenopodium album, Rumex dentatus, constituent which consists of polysaccharides, lipids,
Tinospora cordifolia, Calotropis procera and protein and DNA especially uronic acids, pyruvate, and
Basella alba revealed the high accumulation of Fe, O-methyl, Oacetyl, and sulfate groups. Cyanobacteria
Zn, Cu, Mn, Ni, and Pb in their root and leaves is one of the most efficient microbial community platform
compared to shoot. This indicated high accumulation to naturally synthesize diverse range of therapeutics
and translocation capabilities of these plants. Further, biomolecules. In the present study, six fast growing
the bioaccumulation coefficient factor (BCF) and nitrogen fixing filamentous cyanobacteria representing
translocation factor (TF) was found >1 for majority of the genus of Nostoc, Anabaena and Plectonema
plants for various metals. Thus, this given strong having EPS production in the range of 1.01-1.58 µg ml-
1
evidence for hyperaccumulation tendency of these were identified, biochemically characterized and
native weeds and grasses from complex polluted sites. observed their flocculating activities. Highest levels of
Furthermore, the ultrastructural observations of root EPS were produced by Nostoc sp. when nitrogenase
tissues also revealed the deposition of heavy metals at activity was very low. Maximum EPS production
various cellular components without any apparent toxic occurred at pH 8.0 in the absence of any combined
effects. This indicated the variable adaptive nitrogen source. The cyanobacterial EPS consisted of
characteristics of these plants growing at a hazardous soluble proteins and polysaccharides that included
waste polluted site. Thus, the study given a strong substantial amounts of neutral sugars and uronic acid.
evidence for application of these weeds and grasses Another set of twelve nitrogen fixing cyanobacteria
as tools for in-situ phytoremediation and eco- represent the genus of Nostoc and Anabaena having
restoration of polluted sites contaminated site and EPS production in the range of 0.80-1.66 µg ml-1 were
distillery sludge after phytoremediation showed a characterized. The EPS isolated from Anabaena sp.
sustainable development of ecosystem. and Nostoc sp. demonstrated high flocculation capacity.
Keywords: Native weeds; Endocrine-disrupting chemicals; There was a positive correlation between uronic acid
Distillery sludge; In-situ phytoremediation, â-Sitosterol content and flocculation activity. These strains were
executed for production of EPS, PBS, Chl a and
SIII/O-1 bioflocculant activity. Based on this current global
scenario, the main purpose of this study is to identify
Cultural Studies and Biochemical diverse cyanobacterial regime for its
Characterization in High Yielding biophysicochemical characterization and screening high
yielding EPS and carotenoid producing cyanobacterial
Carotenoids and Exopolysaccharides communities towards commercial exploitation of
Producing Cyanobacteria suitable candidate.
Key words: Carotenoids, Exopolysaccharide, Cyanobacteria,
O.N. Tiwari Ammonia Excretion, Phycobiliproteins.
Centre for Conservation and Utilisation of Blue Green Algae,
Division of Microbiology, ICAR-Indian Agricultural Research SIII/O-2
Institute, New Delhi, 110012, INDIA, Email:
ontiwari1968@gmail.com Beneficial Microbial Services in
Blue green algae or cyanobacteria have been
Management of Agro-Environmental
emerged as a sustainable bioresource of novel bioactive Problems
secondary metabolites. Bioactive secondary
metabolites include value added pigments, food Jay Shankar Singh
colorants, cosmetics, feed additives, polyunsaturated Department of Environmental Microbiology, Babasaheb
fatty acids, exopolysaccharides, vitamins, lipids, Bhimrao University, Lucknow-226025, Uttar Pradesh, INDIA
proteins and therapeutic agents i.e. antioxidants, anti- Email address: jayshankar_1@yahoo.co.in
inflammatory etc. EPSs of cyanobacteria are anionic Land use/cover changes, amongst the most
high molecular weight heteropolymeric extracellular influential environmental issues, are negatively
impacting the soil quality and sustainability. Currently Key words: Agro-environmental sustainability, Ecosystem
it is uncertain how the soil microbial communities and services, Microbial agents, Methanotrophs.
biomass, the foundation of soil fertility support system
will be influenced by anthropogenic mediated land use SIII/O-3
disturbances. It is also not clear how land cover changes
may influence the soil microbial biomass levels and
Isolation, Growth and Identification of
fertility relevance across different soil depths in a given Chloropyrifos Degrading Bacteria
forest or agro-ecosystem. The changes in soil microbial from Agricultural Soil
biomass levels due land use changes may also adversely
affect the soil physico-chemical properties, fertility and Sonal Suman, Satyamvadaswayamprabha and
ecosystem stability. The increasing disturbances in Tanuja Singh*
agriculture and environmental resources due to
urbanization and over exploitation of natural resources Department of Botany and Biotechnology, TPS College,
Kankarbag, Patna-80001, INDIA, Email: sonal.micro89@
create a great concern to the peoples of all over the
gmail.com, tanujasinghbotany@gmail.com
world. The anthropogenic activities led to climate
changes such as drought, flood, and irregular rainfall, Pesticides are a large and varied group of
forest fires, habitat loss, water scarcity, poor agricultural substances that are specifically designed to kill
productivity, soil and environment degradation, etc. organisms including of weeds, insects, and the
These disturbances ultimately impact the sustainability indiscriminate use of pesticides in agricultural field
of agriculture, environment, economy and health. resulted into contamination of soil environment leading
Although global climate change due to increase in green to toxicity. India is an agriculture based country and
house gases such as atmospheric methane (CH 4), maximum portion of it’s economy is dependent on
affect all over the world, it becomes more vulnerable agriculture. The promotion of high yielding varieties of
to developing countries which mainly depend upon crops has led to large scale use of chemicals as
agriculture and agriculture based economy. pesticides. Chlorpyrifos, is one of the most commonly
Management of agricultural yields is a major concern and widely used commercial orgnophosphate pesticide.
for agricultural sustainability, however, until now It is a broad-spectrum, moderately toxic pesticide that
synthetic chemicals have played a fundamental role in has been widely used in the prevention of both
suppressing pests and maintaining high crop yields but agricultural pests and urban public health pests. It was
also responsible for soil degradation and pollution. Soil introduced in 1965 by Dow Chemical Company India.
pollution not only affects the area where it applied but It has large blights on public health and environment
also creates pollution to nearby water bodies, streams resulting from its long residual period in soil and water.
and rivers. They also disrupt the natural ecosystem The microbial action in the environment causes the
and soil microbial diversity, which plays a vital role to natural degradation of the pesticides which might
keep up soil sustainability and health. Bio-agents are convert parent compounds to intermediates or
primarily living microorganisms like fungi, bacteria and comparatively less toxic compounds. The adaptability
cyanobacteria which is used for the controlling of many of microorganisms during bioremediation releases
ecosystem services. These bio-agents provide good certain enzymes, which metabolizes wide spectrum of
agricultural productivity and also enhance soil fertility anthropogenic chemicals. The current method for
through improving natural agro-ecosystem. Some of removing such contaminants from the environment
the bio-agents like cyanobacteria, mycorrhizae, through biodegradation has been shown to be more
methanotrophs, etc., are widely used in restoration of effective than any other method. In the present study,
degraded lands and green house gas -CH4 mitigation. soil sample was collected having history of Chlorpyrifos
Microbial agents are not in optimum use in most from four different soil sample (rice, wheat, maize and
developing countries due to technical, social and vegetable). Nine Chlorpyrifos pesticide utilizing bacteria
institutional constraints. The effectiveness and efficacy were isolated and identified through cultural and
of microbial agents and their resources have yet to be biochemical tests as strains of Bacillus sp.,
explored to become alternate of agro-chemicals. Staphylococcus sp. and Coccus sps. Their growth in
minimal salt medium supplemented with 200 and 250 0.5 mM Cd + PSB. Spinach grown with Cadmium
µg ml-1 of Chlorpyrifos was monitored at optical density showed a decrease in biomass, chlorophyll content and
600 nm. The result showed that Staphylococcus sp., increase in MDA indicating a dysfunction in
Streptococcus sp. and Bacillus sp. had maximum photosynthetic apparatus. Nutrient analysis in spinach
growth at twelve days, while Coccus sp. Gram-ive showed a decrease in sodium (59%), potassium (24%),
Bacillus sp. shows highest growth upto four days of calcium (42%), iron (21%), magnesium (67%),
incubation at 200 µg ml-1 and upto eight days of manganese (32%), copper (22%), nitrogen (18%) and
incubation at 250 µg ml-1 of Chlorpyrifos. The results phosphorus (16%). With higher exposition time, the
of this research indicated that the isolated bacteria can plant was observed to have accumulated 20 mg kg-1 in
be used for bioremediation of Chlorpyrifos shoots and 5 mg kg-1 in roots. The plant showed a strong
contaminated soil. defense mechanism against the oxidative stress with
Key words: Isolation, Bacteria, Chlorpyrifos, Contaminated, the activation of CAT, GPOX and GR indicating the
Agricultural soil. active involvement of glutathione in the plant tolerance
mechanism. While in the plants treated with Cadmium
SIII/O-4 + PSB, there was a decrease in metal accumulation
by 20-25% and increase in phosphorus content by 30%
Potential of Phosphorous Solubilizing as compared to the plants treated with only Cadmium.
Bacteria (PSB) in Improving the Our study indicates that PSBs can influence the
bioavailability of heavy metals in soil, thereby decreasing
Palatability of Vegetable Crops the bioaccumulation in plants.
Grown in Heavy Metal Contaminated Key words: Antioxidative enzyme, Cadmium, Oxidative stress,
Environments PSB, Spinach.
SIII/O-7 SIII/O-8
endophytes were isolated from this medicinal plant also significantly increased shoot biomass without
belonged to twenty-four (24) different species. elevating As accumulation in PvPht1;2 transgenic
Key words: Fungi, Endophytes, Endemic, Artemisia tobacco. Taken together, our results demonstrated that
amygdalina, Kashmir Himalayas. PvPht1;2 is a specific P transporter responsible for P
acquisition and translocation in plants. On the other
SIII/O-9 hand, enhancing PvPht1;3 increased arsenate uptake
and translocation in tobacco plants. As concentrations
Identification and Functional Analysis in shoots of PvPht1;3-Oxwere 29-38%higher after
of Phosphate Transporters in AsV exposure for 1day than that of WT, respectively.
AsV uptake rate, As concentration in xylem sap and
Phosphate and Arsenate Absorption translocation factor were increased compared to wild
and Transport in Pteris vittata type plants with different degree, respectively. It’s
suggested that PvPht1;3 played key role in efficient
Yue Cao*, Dan Sun, Yanshan Chen and Lena Q. absorption and transport of arsenic in Pteris vittata.
Ma In this study, diverse functions of Pteris vittata PHT
transporter in arsenate and phosphate uptake pathway
State Key Lab of Pollution Control and Resource Reuse,
School of the Environment, Nanjing University, Nanjing, were preliminarily identified. We expect to use the key
Jiangsu 210023, CHINA, Email: caoyue@nju.edu.cn gene, PvPHTs, to improve the efficiency of
phytoremediation in future.
Pteris vittata, the first arsenic hyperaccumulator
identified, has very strong ability to absorb the arsenic, Key words: Pteris vittata, Nicotiana tabacum,
Phytoremediation, Transcriptome, Hyperaccumulator.
so it is widely used in phytoremediation of arsenic
contaminated soil. However, the molecular mechanism
SIII/O-10
of arsenic uptake efficiency of Pteris vittata is not
clear. As a phosphorous analog, arsenate is absorbed Sustainable Solutions for Arsenic
mainly by the phosphate transporter Pht1. Using the
transcriptome sequencing and gene cloning, five Stress Mitigation and Arsenic
members of Pteris vittata Pht1, have been identified. Reduction in Rice
We determined their arsenic transport capacity through
the analysis of arsenic content in different Pht1 strains Sudhakar Srivastava
of yeast transformation lines. By qRT-PCR, expression Plant Stress Biology Laboratory, Institute of Environment
pattern of PvPht1 protein have been identified, and Sustainable Development, Banaras Hindu University,
PvPht1;2 and PvPht1;5 transcripts were induced to Pi Varanasi-221005, INDIA, Email: sudhakar.srivastava@gmail.com
starvation and PvPht1;4 expression level was increased
in response to As exposure. PvPht1;2-PvPht1;5 were The problem of geogenic arsenic contamination
all localized to plasma membrane, by expression in is widespread in Gangetic plains in Indian states of
tobacco leaves with GFP fusion protein, respectively. Uttar Pradesh, Bihar and West Bengal. In this region,
Further, PvPht1 transporters were transformed into arsenic concentration of groundwater is several folds
tobacco (Nicotiana tabacum), respectively. In higher over the safe limit and toxic levels of arsenic
hydroponic experiments, all PvPht1;2-Ox lines have been reported in crop plants, vegetables, fruits
displayed markedly higher P content and better growth and fish and meat products. In the affected states, rice
than wild type plants, suggesting that PvPht1;2 mediated is the main crop and staple food for humans. Rice is
P uptake in plants. In addition, expressing PvPht1;2 more efficient in arsenic accumulation in its grains than
also increased the shoot/root 32P ratio by 69-92% and other cereal crops. The problem can be tackled either
enhanced xylem sap P by 46-62%, indicating that through remediation of contaminated water or through
PvPht1;2 also mediated P translocation in plants. Unlike management of agronomic practices for growing safe
many Phts permeable to AsV, PvPht1;2 showed little rice. Further, there are known variations in rice
ability to transport AsV. In soil experiments, PvPht1;2 genotypes in terms of arsenic accumulation in their
grains. The grains of these contrasting genotypes show their relationship and the underlying mechanisms have
variations in accumulation of other mineral elements not been investigated. From this backdrop, pot
as well as in amino acid contents. Hence, genotype experiments were carried out to determine interplay
screening and selection and development of suitable between Zn (500 and 1000 mg kg-1 dry soil) and AM
genotype for cultivation in affected regions are fungus (Funneliformis mosseae) on growth, nutrient
promising approaches. With respect to agronomic acquisition, oxidative stress and non enzymatic
practices, supplementation of rice crops with redox antioxidants in two pigeonpea [Cajanus cajan (L.)
active chemical, thiourea, has been proved to be a Millsp.] genotypes (Tolerant- Sel 85N and Sensitive-
promising approach in lab conditions. In addition, other P792) exposed to Cd stress (25 and 50 mg kg-1 dry
chemical (nitrogen) and biological (microbial consortia) soil). Results revealed that accumulation of Cd and Zn
amendments could pave way for reducing arsenic in reduced plant biomass, disrupted ionic balance by
rice grains. Hence there lies possibility of its application interfering with the uptake and translocation of various
in field conditions for safe rice cultivation. The nutrients (N, P, K, Mg, Ca, Fe), induced membrane
presentation highlights key findings in the direction of destabilization, hydrogen peroxide (H2O2) accumulation
suggested possible approaches to tackle the issue of and increased non-protein thiols (NP-SH) [glutathione
arsenic contamination of rice. (GSH), glutathione disulphide (GSSG) and
Key words: Arsenic, Genotype, Nitrogen, Microbial consortia, phytochelatins (PCs)], with Cd being more toxic in
Rice. P792 than Sel 85N. Zn supplementation reduced the
uptake of Cd which further declined when plants were
SIII/O-11 co-inoculated with F. mosseae that resulted in increased
nutrient pool, decreased reactive oxygen species (ROS)
Zinc-Funneliformis mosseae generation via enhanced production of GSH and PCs,
Composite Application Attenuates ultimately improving growth. These findings suggested
the integrated application of Zn and AM as a promising
Cadmium Toxicity in Pigeonpea via management strategy to ameliorate Cd toxicity in
Modulating ROS Generation and pigeonpea.
Enhanced Production of Glutathione Key words: Arbuscular mycorrhiza, Cadmium, Glutathione,
Pigeonpea, Phytochelatins, Zinc.
and Phytochelatins
SIII/O-12
Harmanjit Kaur1* and Neera Garg2
1
Department of Botany, Akal University, Talwandi Sabo, Prospectives of Trichoderma sp. in
District: Bathinda, Punjab-151302, INDIA; 2Department of Amelioration of Arsenic Induced
Botany, Panjab University, Chandigarh-160014, INDIA, Email:
harmanjit.garcha@yahoo.com, garg_neera@yahoo.com Phyto-Toxicity
Cadmium (Cd) is a widespread toxic trace Pratibha Tripathi 1,2 *, R.D. Tripathi 2 and Alok
element, non-essential for plant growth and is found in Kalra1
agricultural soils due to various anthropogenic activities.
1
However, Zinc (Zn) is an essential micronutrient at CSIR-Central Institute of Medicinal and Aromatic Plants,
low concentrations but toxic to plants at higher Lucknow-226001, INDIA; 2CSIR-National Botanical
Research Institute, Lucknow-226001, INDIA, Email:
concentrations along with Cd. Zn interferes with Cd
pratibhaa25686@gmail.com
transport by competing with Cd for binding sites of a
common transport protein on root plasma membrane. Arsenic (As), a toxic metalloid, is a food chain
Arbuscular mycorrhizal (AM) symbiosis is considered contaminant and is threat to all living forms. Irrigation
a potential biotechnological approach for increasing with As-contaminated groundwater has increased As
plant tolerance to heavy metal contamination. accumulation in agricultural soils which is a potential
Composite application of excess Zn and AM fungi can health risk for humans. Plant-microbe interaction is a
increase plant resistance to Cd toxicity, nevertheless, promising eco-friendly strategy for conventional and
organic agriculture. Microorganisms have developed growth and development of vegetable and ornamental
different mechanisms to transform toxic forms of As plants. Use of Trichoderma spp. as bio control agents
to less toxic forms. In present study, role of As tolerant as well as growth promoters in agricultural systems
Trichoderma reesei NBRI 0716 in countering the As has been identified as an effective methodology to
induced stress in chickpea (Cicer arietinum) plants minimize the use of agrochemicals. Use of problematic
was studied. Arsenic exposure significantly hampered invasive plants together with agricultural wastes as
the growth, uptake of mineral nutrients and antioxidant organic materials facilitates proper recycling under
activity in chickpea resulting in poor pod setting and sustainable agriculture. Three Trichoderma spp. (T.
limited seed yield. Inoculation of chickpea with T. reesei virens, T. harzianum and T. asperullum) were
significantly promoted plant growth, yield and mineral selected and mass produced separately, using 250 g of
nutrients both in absence and presence of As. sawdust as the carrier material. Four different potting
Trichoderma inoculation also helps in restoration of media (coir + invasive plants + poultry manure with
stem anomalies like reduced trichome turgidity and rice husk; T-1, coir + invasive plants; T-2, coir +
density, deformed sclerenchymatous and compost; T-3 and compost + field soil; T-4) were
collenchymatous cells induced by As stress. prepared. Next, all the potting media were divided into
Upregulation of drought responsive genes, enhanced two parts and one set was treated with Trichoderma
proline content and shrinked cortex cells in presence spp. while the other set was used as controls, without
of As were modulated by T. reesei. In addition, adding Trichoderma. Physical and chemical properties
inoculation of T. reesei lead to three fold reduction of of the four potting media were analyzed following
inorganic As and enhanced dimethylarsinic acid and standard methods. Effect of Trichoderma amended
mono-methylarsonic acid content in soil and seed as potting media on plant growth was evaluated using
well, indicated that T. reesei probably has a role in Abelmoschus esculentus by measuring several growth
methylation of As as the possible mechanism for parameters (plant height, number of flowers and fruits
maneuvering As stress in chickpea. Therefore, yield) and disease incidence under greenhouse
bioremediation and PGP potential of T. reesei makes it conditions. Of the four potting media, T-1 exhibited
a promising bio-inoculant for application in As affected optimum physical properties i e; porosity (45.2%), bulk
sites and production of safe farm produce. density (0.34 gcm-3) and water saturation capacity
Key words: Arsenic, Bioremediation, Chickpea, Trichoderma, (52.4%); as well as chemical properties i.e. pH (6.8),
Stress. EC (0.89 ds m-1), organic carbon (17.2%), N (1.35%),
P2O5 (1.23%), K2O (1.48%) and C/N ratio (15.8). The
SIII/O-13 highest mean plant height (85.8±2.5 cm), number of
flowers per plant (18±2) and fruits yield (125.5±3.5 g)
Formulation of an Organic Potting were observed in plants treated T-1 medium with
Medium Incorporated with Trichoderma amendments. A. esculentus planted
without Trichoderma treatment in T-4 showed
Trichoderma spp. to Enhance Growth significantly lower (p<0.05) growth than T-1 and T-3
and Disease Suppression of media. A. esculentus grown in Trichoderma amended
T-1 medium showed a significantly lower (p<0.05)
Abelmoschus esculentus disease incidence (5%) compared with untreated
controls. Based on the results (T-1) was selected as
N. Hewavitharana*, S.D.P. Kannangara, L.R.
most effective medium and was prepared as a brick to
Jayasekera and S.P. Senanayake
facilitate the easy handling as well as for and
Department of Botany, Faculty of Science, University of commercialization of the product in order to introduce
Kelaniya, SRI LANKA, Email: nalakahewavitharana to both local and export markets.
@gmail.com
Key words: Agriculture, Invasive plants, Coir dust,
Organic potting media is an important source for Trichoderma, Abelmoschus esculentus.
SIII/O-14 SIII/O-15
orthologs of rice OsHKT1;4 and OsHKT1;5. Orthologs property in the area of biotechnology helps to accelerate
of OsHKT1;1 and OsHKT 1;3 were included in the economic and social development for a developing
main cluster II and the third main cluster included country like India. However, there are IP concerns in
orthologs of OsHKT2;1 and OsHKT2;4. Interestingly developing countries and a need for greater protection
the genes that exist as neighboring pairs were clustered and access. Isolation of live organisms e.g. bacteria
in different main clusters indicating that tandem gene etc. simply from their habitat were not eligible for
duplication and subsequent divergence could be patents. Biotechnology and genetic engineering makes
potential mechanism in the evolution of HKTs. it possible to include such biotechnological products.
However, G block predictions identified highly Also the patenting of life forms is unlike mechanical
conserved sequence fragments among the HKTs, inventions and thus poses ethical issues in biotechnology.
specifically in the P-loop domains identifying potential Key words: Patent, Innovation, IPR, Intellectual Property,
functional sites. These findings provide a basis to GI, Economy.
illuminate on the potential functional sites on HKTs.
Key words: Salinity, Granimae, Genome databases, BLAST, SIII/O-19
G block.
Analysis of HKT Homologs in Grasses
SIII/O-18 by Comparative Genomics and Protein
Intellectual Property Rights and Structure Prediction
Biotechnology Issues M.H.M.M.N. Mapa1,2 *, P.R. Weerasinghe1,3 and
H.A.C.K. Ariyarathna1
Saurabh Yadav
1
Department of Botany, University of Peradeniya, Peradeniya,
Department of Biotechnology, Hemvati Nandan Bahuguna SRI LANKA; 2Sara Bhumi Lanka Bio Products (Pvt.) Ltd,
Garhwal (Central) University, Srinagar Garhwal-246174, Dambrawa, Pilawala, SRI LANKA; 3Coconut Research
Uttarakhand, INDIA, Email: saurabhyadav40@rediffmail.com Institute, Bandirippuwa Estate, Lunuwila, SRI LANKA
Everyone desires to acquire a property, mostly Email:nisansala.mapa1@gmail.com, pabasaraweerasinghe@
tangible ones. But intangible property have also much yahoo.com, ckariyarathna@yahoo.com
value, since it is having long term effect. One of the
Soil salinity is a major abiotic stress that incurs
intangible property is Intellectual property. Intellectual
economic losses in crops. High affinity K+ transporters
property is generated by the application of human
(HKTs) are a class of integral membrane proteins
intellect. ‘Intellectual property’ is the dynamic area
(IMPs) involved in Na+ transport and some members
due to the emergence of new knowledge and
of this group are candidate proteins associated with
technologies. The ‘brick and mortar economy’ deals
salinity tolerance. This analysis aims in-sillico prediction
with all the properties such as land, house etc. The
of potential functional sites in this group of proteins
‘Knowledge economy’ is the focus of the worldwide
based on evolutionary significance of the different
economy, in current scenario. IP protection is mainly
domains. Fifty-one orthologous protein sequences from
bypatents, copyrights, geographical indications,
Brachypodium distachyon, Sorghum bicolour
trademarks, trade secrets, know-how etc.
(Sorghum), Setaria italica, Zea mays (Corn),
‘Biotechnology’ deals with living forms and inventions
Hordeum vulgare (Barley), and Triticum aestivum
related to biotechnology must be dealt with extra care
(Wheat), were retrieved by quarrying genome
to avoid legal issues and needs to be protected by
databases using eight previously known rice (Oryza
intellectual property rights. Inventions related to
sativa) HKT proteins as the queries. The conserved
biotechnology seems to be promising for industrial
domains in the proteins were identified by G Blocks
productivity. The legal battle related to turmeric, basmati
(www.idtdna.com) prediction. Protein 3D structures
rice and neem are examples of strict IPR laws. Non-
were obtained by homologous protein prediction by
patentable biotechnology inventions and also legal issues
PYRE2 and analyzed by PYMOL viewer. The
like ethics, morality etc. Protecting the intellectual
conserved domains were studied in the 3D structures. use a set of Arabidopsis GUS mutation detector lines
Three highly conserved domains were identified where to study the impact of anthropogenic activities on
group mean pair wise distance was 0.451. Based on genome stability. Our research has revealed that the
structure predictions these domains were found in the two crop protection methods - genetic transformation
first, second and third Trans- membrane domain and and pesticide application, have opposite impact on
the P loop regions. These three domains predict the somatic mutations like homologous recombinations,
potential functional sites in HKTs that can be further frame-shift and point mutations. Both the stresses
studied to illuminate on mechanisms of iron transport caused epigenetic changes, as analysed by methylation-
and to identify functional variants among the proteins sensitive restriction digestion PCR, in the promoters of
to enable utilizing these genes in improvement of salinity certain DNA repair genes involved in recombination,
tolerance in plants specially the important cereal crops. base-excision repair or nucleotide-excision repair
Key words: Salinity tolerance, Homology prediction, Protein pathways. To the best of our knowledge, there are no
3-D structure, Functional domains. previous studies on such an epigenetic analysis of DNA
repair system. Our approach provides an excellent
SIII/O-20 platform to study transgenerational memory of other
environmental hazards as well.
Molecular Basis of How Plants can be Key words: Transgenerational memory, Epigenetics,
Used as a Potential Detector System Mutation, Methylation, DNA repair.
to Analyse Environmental Hazards SIII/O-21
Jasmine M. Shah*, J. Sijila and T.J. Joyous Effect of Fly Ash on Growth of
Department of Plant Science, Central University Kerala, Cellulolytic Bacteria
Kasaragod, Kerala-671316, INDIA, Email: jasmine@cukerala.
ac.in, sijila.janardhanan@gmail. com, joyoustj@gmail.com
M.S. Dhanya* and P. Ashoka Chakravarthy
Plants are sessile organisms and are forced to Department of Environmental Sciences and Technology,
face the injustices imposed by environment and Central University of Punjab, Bathinda-151001, Punjab,
anthropogenic activities. Stress perceived by any INDIA, Email: dhanyasubramanian@gmail.com,
organism, is often evidenced by a physiological change. ashokachakravarthy987@gmail.com
Changes when occur in animals, often turn out to be
The cellulose is the most abundant material on
lethal whereas, plants can still thrive with an
the earth. This is the major component of organic wastes
abnormality. For example, sterility, a phenotype which
such as kitchen waste and requires waste management.
brings an end to a generation in animals, can be easily
The cellulolytic bacteria play an important role in
ignored by a vegetatively propagated flora. Phenotype
maintaining the ecosystem balance by means of
is nothing but manifestation of the genotype. Recent
decomposition. The hydrolysis depends on cellulolytic
research has revealed that not only genetic, but also
microbes which is overall rate limiting step in
epigenetic changes reflect into the making of a
composting and anaerobic digestion. The fly ash is an
phenotype. DNA, the genetic basis of life, is highly
inorganic waste that is generated from thermal power
conserved across the life forms. More importantly, the
plants as a major pollutant. The disposal of food waste
mechanisms of DNA replication, repair and epigenetic
and fly ash are of great concern. The focus of the
regulation are highly conserved across the eukaryotic
study was to evaluate the effect of fly ash on the growth
world, from yeast to plants to mammals. Hence, plants
of cellulolytic bacteria involved inorganic waste
offer an excellent system to monitor the molecular
digestion. The fly ash addition to food wastes enhanced
impact of environmental hazards, both genetic and
the cellulase activity and biomass growth of cellulolytic
epigenetic, simply because of ethical reasons and easy
bacteria. The fly ash addition to rice wastes increased
handling. Our group works on genomic stability,
cellulase production by cellulolytic bacteria than roti
expression and epigenetic regulation of DNA repair
wastes. The fly ash improved the manorial efficiency
and transgenerational memory of plant genome. We
of food wastes and helps in production of nutrient rich approach in order to enhance the beneficial effects of
manure that can be used for plant growth. Si in chickpea. Conclusively, these results support usage
Key words: Fly ash, Cellulolytic bacteria, Rice waste, Roti of Si along with AM as potent candidates for alleviating
wastes, Sustainable waste management. salt-induced osmotic stress.
Key words: Chickpea, Mechanism(s), Salinity, Silicon,
SIII/O-22 Proline, Soluble sugars.
(Aspergilus niger) and it was found that the extract in PGPR and its significance as an alternative of
of the plants grown in vivo was much less effective chemical fertilizers towards sustainable agriculture.
as an antifungal agent than the extract obtained from Key words: Crop yield, Plant growth promoting rhizobacteria
those grown in vitro by the Minimum Inhibitory (PGPR), Sustainable agriculture.
Concentration (MIC) test. (Note: MS - Murashige and
Skoog, PGR – Plant growth regulator, BAP – Benzyl SIII/P-2
amino purine, 2.4 D – 2-4 dichloro phenoxy acetic acid)
Key words: Nodal explants, MS, Callus, Antifungal activity, Indirect Organogenesis from Leaf
Flavonoids, MIC. Segments of Solanum lycopersicum
SIII/P-1 Poonam Chaudhary 1 *, Balwinder Singh 2 and
Avinash Kaur Nagpal1
Plant Growth Promoting 1
Department of Botanical and Environmental Sciences, Guru
Rhizobacteria: Prospects towards Nanak Dev University, Amritsar-143005, Punjab, INDIA;
Sustainable Agriculture 2
Post Graduate Department of Biotechnology, Khalsa
College, Amritsar, INDIA, Email: poonamchaudhary73@
Ashmita Ghosh 1 , Mrinal Ray 1 , Debnirmalya yahoo.com, bbs171@rediffmail.com, avnagpal@
rediffmail.com
Gangopadhyay1* and Suman Das2
1
Department of Sericulture, Raiganj University, Raiganj, Uttar
Series of experiments were performed to test the
Dinajpur-733134, West Bengal, INDIA; 2Deptartment of effect of different concentrations of 2,4-
Handloom Handicrafts and Sericulture, Sericulture Training dichlorophenoxy acetic acid (0.1-1.0 mg l-1) alone and
Institute, Tripura, INDIA, Email: deb_ganguly2003@ in combination with kinetin (0.5 mg l-1) on indirect
yahoo.com organogenesis response from leaf segments of
Solanum lycopersicum. Callus initiation was observed
The chemical fertilizers and pesticides not only
on leaf segments in all the treatments from 7-10 days
create environmental and human health problems but
after inoculation. Maximum callus induction (90%) was
also greatly affect the soil health. It has been reported
observed on MS medium supplemented with 0.1 mg l-
that regular use of chemical fertilizers brings down the 1
2,4-dichlorophenoxy acetic acid and 0.5 mg l-1 kinetin.
productive capacity of the soil. Therefore, the soil with
The in vitro response of explants for callus induction
poor productivity often results in low profit particularly
was recorded on 28th day after inoculation and it was
for the marginal farmers who viciously tied to take loans
found that the calli were white and compact. The calli
to meet the rising cost of agrochemicals. Of late, the
were cut into small pieces and inoculated onto
importance of plant growth promoting rhizobacteria
regeneration medium [MS medium containing different
(PGPR) attracted the attention of the researchers.
concentrations of 6-benzylaminopurine (0.1-2.0 mg l-
Various studies revealed that PGPR helps in increasing 1
)]. Maximum shoot regeneration (76%) was observed
crop yield by improving soil fertility, accelerating plant
on medium supplemented with 0.25 mg l -1 6-
growth and suppressing phytopathogens etc. The
benzylaminopurine. Shoots were healthy and achieved
physiology of plant gets affected by PGPR through
the length of 6.18±1.75 cm within 4 weeks. Shoot
various mechanisms like phytostimulation (via
regenerated on MS medium containing 6-
production of gibberellins, indole acetic acid, ethylene
benzylaminopurine (0.1-0.75 mg l-1) was shown to root
and cytokinins), biofertilization (through phosphate
as well without the addition of auxins. This study reveals
solubilization, biological fixation of atmospheric nitrogen,
that transfer of calli (raised on medium containing 0.1
exopolysaccharides production and siderophores
mg l-1 2,4-dichlorophenoxy acetic acid and 0.5 mg l-1
production); and bio-defense mechanisms (via systemic
kinetin) to MS medium with 6-benzylaminopurine 0.25
resistance, competition for nutrient and space,
mg l-1 was the best option to obtain complete plantlets.
production of lytic enzymes, antibiotics, hydrogen
This experiment would be useful to create somaclonal
cyanide) etc. The study illustrates the latest advances
variations and also for utilizing transgenic approaches indicator lichen species can be used for monitoring of
to improve tomato varieties. environmental quality in the study area.
Key words: Solanum lycopersicum, Somaclonal variations, Key words: Bio-indicator, Heavy metal, Nitrophile, Pollutant,
MS medium. Toxi-tolerant.
SIII/P-3 SIII/P-4
alcohol-to-oil molar ratio. So, these chemical catalysts such as Pseudomonas sp., Aspergillus versicolor,
may replace by lipase enzyme. Lipase have the capacity Saccharomyces cerevisiae, Cunninghamellla
to performed transesterification reaction. Lipase elegans, Microbacterium profundi, Cladosporium
produced from number of microorganisms such as are widely used (alone or in combination) for
bacteria, fungi etc. Lipase from fruit waste also used bioremediation of heavy metals from metal
in biodiesel production. The use of lipase will minimize contaminated sites. Moreover, associations of plant
the chemical catalysts. So, the enzymatic based roots with soil microorganisms such as rhizobium and
biodiesel production is the emerging research area for arbuscular mycorrhizal fungi have proved to be a
grow toward green chemistry and sustainable promising strategy for imparting metal stress tolerance
development. in crop plants for sustainable agriculture. In this review
Key words: Energy, Biodiesel, Transesterification, Catalyst, poster, we explore the difference mechanisms
Lipase. undertaken by plants and microbes in response to heavy
metal stresses.
SIII/P-8 Key words: Heavy metals, Bioremediation, Rhizobium,
Arbuscular mycorrhizal fungi.
Heavy Metal(loid) Stresses in Plants
and their Detoxification by Soil SIII/P-9
Microorganisms Trichoderma Mediated Modulations
Ipsa Gupta* and Neera Garg of Chickpea Rhizospheric Activities in
Department of Botany, Panjab University, Chandigarh- Presence of Organic Amendments and
160014, INDIA, Email: garg_neera@gmail.com, ipsagupta Inorganic Fertilizers
1996@gmail.com
Heavy metal(loid) (HMs) including arsenic, lead, Anshu*, Ashmita Tandon, Udit Uadav, Pankaj
nickel, cadmium, chromium and mercury are important Kumar Srivastava, Kumkum Mishra and Poonam
environmental pollutants that are increasing across the C. Singh
globe due to geologic and anthropogenic activities. Their CSIR-National Botanical Research Institute, Rana Pratap
presence in the soil leads to increase in oxidative stress, Marg, Lucknow-226001, INDIA, Email: anshugautam1808
degradation of plant growth cycles (including @gmail.com
embryogenesis, organogenesis), oxidation of protein, Interaction of plant growth promoting
inactivation of enzymes, DNA damage etc. Plants microorganisms (PGPMs) with plants interchanges
respond to HM toxicity in a variety of different ways signals at physical, physiological and molecular levels.
such as immobilization, exclusion, chelation and Proliferation and root colonization of PGPMs contribute
compartmentalization of the metal ions, and the in improving plant functions by manipulating the
expression of more general stress response mechanisms rhizosphere. They benefit plants through different
such as ethylene and stress proteins however, these mechanisms like nutrient enrichment and induction of
mechanisms are scarcely effective under high plant vigor and defence system. The present work aims
concentrations of metals. Several physical techniques to decipher the rhizosphere modulations by an organic
such as adsorption and chemicals like alginates, citrus acid producing Trichoderma strain (NBRI-PR5) under
pectin, flavonoids and phytic acid are being used to different fertilization regimes. Chickpea reciprocates
remove heavy metal contaminants from the thoroughly to the application of organic amendments,
environment which however are costly, mechanically inorganic fertilizers, and NBRI-PR5. The determining
complex and time consuming. Bioremediation is, factor in the study was the microbial communities
therefore, an eco-friendly and efficient method of associated with the plant rhizosphere which was
reclaiming metal(loid) contaminant environments by determined by culturable populations of heterogeneous
making use of the inherent biological mechanisms of microflora (Bacteria, fungi, and Actinomycetes) .The
microorganisms and plants. In this technology, microbes
rhizospheric functions were studied by determining the LWG002611 possesses suitable biodiesel properties as
soil enzyme activities and HPLC profiles of organic per the range defined by the European biodiesel
acids in root exudates. The application of NBRI-PR5 standard EN14214 and petro-diesel standard
strain induced favourable changes in rhizosphere upon EN590:2013. The whole genome sequencing (WGS)
the application of amendments. The minimum bacterial predicted a total of 13514 genes from de novo and
CFU noticed in the NPK treatment alone (4.18 Log10 16739 genes from reference guided assembly. The
CFU g-1) confirming the inhibitory effects of chemical genome size was estimated at 65.35 Mb. Metabolic
fertilizers on rhizosphere microflora compared to the pathways including lipid metabolic pathways were
unamended control (6.71 Log10 CFU g-1). Application reconstructed and a total of 283 genes were identified
of NBRI-PR5, with organic amendments or inorganic in lipid metabolism. Multiple gene homologs were
fertilizers lower the pH of the rhizosphere causing identified in triacylglycerol (TAG) biosynthesis pathway.
inhibition of alkaline phosphatase activity in the The phylogenetic analysis from WGS revealed that the
treatments compared to the unamended control strain belongs to class Chlorophyceae and order
(0.5µgpNP.g-1 soil.h-1 ). NBRI-PR5 had refurbished Sphaeropleales. Molecular identification was carried
microbial activities to facilitate nutrient uptake in plants out by sequence of small subunit ribosomal RNA gene
rather than fix in the microbes which is demonstrated (18S rRNA) and validated through morpho-taxonomy.
by an opposite trend in DHA and protease enzyme Taken together, this first report on WGS of S.
activities in the rhizosphere of FYM and FYM+PR5 quadricauda and an insight into the lipid biosynthesis
treated plants. The conclusion of the study is that pathway could identify the targets for genetic and
NBRI-PR5 momentously regulates rhizosphere metabolic engineering to further improvement of the
activities in terms of enzymatic and microbial activities strain and useit as a sustainable source of biodiesel.
of the rhizosphere, distinct to different fertilization Key words: Secenedmus quadricauda, Biodiesel properties,
regimes by varying the enzyme activities to maximize Whole genome sequencing, Lipid biosynthesis pathway,
the utilization of available nutrients. Phylogenetic analysis.
Key words: Soil enzymes, Organic acids, Rhizosphere
remodelling, Chickpea, Trichoderma. SIII/P-11
Langra, cv. Zill, cv. Shelly and cv. Kent from Pakistan, properties through the modulation in physico-chemical
China, Israel, and Mexico respectively. There are parameters viz. pH, time, temperature, concentration
30,953-85,036 unigenes identified from RNA-Seq of cell free extract, and gold salt during biosynthesis of
datasets of mango cultivars in De-novo sequence gold nanoparticles. A varying size ranges from 2–500
assembly. The significance of this specific tools is to nm with different shapes i.e. spherical, triangular,
provide the scientific community and general public a nanoprisms, pentagon/hexagon and nanosheets were
mango genomic resource and allow the user to examine synthesized. Catalytic activity of these gold
their data against our analyzed mango genome nanoparticles were evaluated by UV-visible
databases of four cultivars (cv. Langra, cv. Zill, cv. spectroscopy followed by gas chromatography-mass
Shelly and cv. Kent). A mango web genomic resource spectrometric analysis. Spherical nanoparticles of size
MGdb, is based on 3-tier architecture, developed using 3-10 nm were showing highest catalytic activity among
Python, flat file database, and JavaScript. MGdb is a different shape and size nanoparticles. Decreases in
website which contains the information of predicted size and spherical shape strongly affect the adsorption
genes of the whole genome and the unigenes annotated and activation of the reactants. An attempt has been
by homologous genes in other species, GO (Gene made to obtain unique balance of physical parameters
Ontology) terms which provide a quick look of the traits to set a strategy for selection of specific structures of
in which they are involved. This web based portal gold nanoparticles for enhancing catalytic properties
belonging to different mango cultivars could be a in degradation of organic pollutants.
millstone in the assessment of the research, Key words: Gold nanoparticles, Green technology,
development of the medicines, understanding genetics Trichoderma.
and provides useful bioinformatics solution for analysis
of nucleotide sequence data. We report here world’s SIII/P-13
first web-based genomic resource particularly of mango
for analysis and comparative studies of different mango Histopathological Studies of Drought
cultivars. Tolerant and Sensitive Rice Varieties
Key words: RNA-seq, Annotation, Gene Ontology, in Presence of Endophytes under
Phylogeny.
Drought Condition
SIII/P-12
Touseef Fatima*, Isha Verma, Sanjeev Kumar and
Synthesis of Bioengineered Gold Poonam C. Singh
Nanoparticles for Enhancing Catalytic CSIR-National Botanical Research Institute, Lucknow-
226001, INDIA, Email: taus.fatima@gmail.com
Activity
There exists a large variability in root system
Ashutosh Tripathi*, Shipra Pandey, Ved Prakash architecture in cultivated rice varieties which
Giri, Vivek Pandey and Aradhana Mishra consequently determines its drought tolerance ability.
Division of Plant Microbe Interactions, CSIR-National Roots are sensitive to abiotic and biotic stresses and
Botanical Research Institute, Rana Pratap Marg, Lucknow- respond at all levels of plants’ structure and function
226001, INDIA, Email: mishramyco@yahoo.com including morphological and anatomical traits. The
drought tolerance or avoidance in rice is largely
Biosynthesis of nanoparticles is an emerging
considered to be dependent on the deep rooting seminal
technology that is green, cost-effective and eco-friendly
and primary roots and are the targets for breeding
but the optimization for its maximum activity is doubtful
varieties better adapted to drought. To understand the
thus there is a need of improvisation for maximum
morphological plasticity, which affect the water
catalytic activity. The main aim of our work is to
harvesting property we studied the morphological and
synthesize gold nanoparticles by cell free extract of
anatomical differences induced by PEG6000 in two
Trichoderma viride, a reducing and stabilizing agent
contrasting rice varieties, Heena (drought resistant) and
with effective potency and improved catalytic
Kiran (drought sensitive), in both absence and presence mg kg-1 dw) and serine (17.53 mg kg-1 dw) were
of rice endophytes. The Morphological and Anatomical significantly higher in seeds of fusant inoculated
features of Heena and Kiran varieties were studied in chickpea. Mineral contents like i.e. Cu (157.73 mgkg-
1
presence of 20% PEG6000. The sampling was carried dw), Co (0.06 mg kg-1 dw), Ni (1.85 mg kg-1 dw), Zn
out 1, 3, 5, 7 and 10 days after PEG6000 treatment. (157.73 mg kg-1 dw) and S (16.29 mg kg-1 dw) were
The sensitive variety appears to give a spontaneous significantly improved. Fusant inoculated plants showed
response and immediately starts increasing the root better biocontrol and defence activities in chickpea from
length similar to hydrotropism to explore deeper water 20 to 35% during biotic stress. This is one of the first
while the tolerant one is more planned to first increase report on increase in amino acids and mineral content
the root numbers which are structurally and of chickpea by fusant inoculation.
physiologically adapted to drought and then grow in Key words: Protoplast fusant, Trichoderma, Amino acids,
size to get maximum benefit. Increased prevalence of Nutritional value, Biocontrol.
tight packing of cells in the exodermis and lignified
sclerenchyma layer which improves its effectiveness SIII/P-15
as a barrier and prevent loss of water was observed in
presence of the microbial intervention. Increased Molecular Diversity and
lignification and thickened walls in presence of bacteria Characterization of Indigenous Salt
make large negative pressures possible and help in
water uptake. Tolerant Plant Growth Promoting
Key words: Rice, Drought, Endophyte, Heena, Kiran. Rhizobacteria Isolated from Different
Agro-Climatic Zones of Uttar Pradesh
SIII/P-14
Sankalp Misra, Shashank Kumar Mishra, Vijay
Modulation in Nutritional Value and Kant Dixit, Swati Gupta and Puneet Singh
Defence Activity of Chickpea by Chauhan*
Supplementation of a Novel Division of Plant Microbe Interactions, CSIR-National
Botanical Research Institute, Lucknow-226001, INDIA,
Trichoderma Fusant Email: puneetnbri@gmail.com
Pratibha Verma*, Nishtha Mishra, Priyanka A extensive study for 9 agro-climatic zones of
Chauhan and Aradhana Mishra Uttar Pradesh, India was conducted for the isolation
and characterization of salt tolerant 1-
CSIR-National Botanical Research Institute, Lucknow-
226001, INDIA, Email: mishramyco@yahoo.com
aminocyclopropane-1-carboxylic acid (ACC)
deaminase possessing plant growth promoting (PGP)
Due to rise in global population and food rhizobacteria for salt stress amelioration in rice. For
consumption, yield of agricultural crops has to be this study, we have isolated 1125 bacteria having the
increased substantially in the coming years. To fulfill ability to tolerate 1M NaCl and screened for utilizing
the dietary requirements diminishing nutritional value ACC as sole nitrogen source. The resultant 77 isolates
of the grains has to be maintained by improving the were further evaluated for seed germination assay, PGP
agricultural practices. Legumes and cereal crops are and abiotic stress tolerance ability in vitro. This
important staple food crops with high nutritional values evaluation revealed 15 potent rhizobacteria representing
in amino acids content, carbohydrate content etc. In each agro-climatic zone and salt stress mitigation in
the present study we have used a protoplast fusant vitro. In particular, the biomass obtained for bacteria
(H. lixii MTCC 5659) for enhancing the nutritional value coated rice seedlings were corroborated with the
and defence activity in chickpea. Essential amino acids; performance of isolates exhibiting maximum IAA
methionine (9.82 mg kg-1 dw), cysteine (2.61 mg kg-1 production. Surprisingly based on 16S rRNA, much of
dw), glycine (11.34 mg kg-1 dw), valine (9.26 mg kg-1 the propitious isolates belonged to same specific epithet
dw), and non-essential amino acids; aspartic acid (39.19 exhibited variedly in their characteristics. Overall,
Bacillus spp. was explored as dominant genera in toto metal contamination sites. ã-Proteobacteria was found
with highest distribution in Western plain zone followed to be the most abundant bacterial community followed
by Central zone. Therefore, this study provides a by Actinobacteria, Firmicutes, â-Proteobacteria and á-
counter-intuitive perspective of selection of native Proteobacteria. Overall, the present study leads to a
microflora for their multifarious PGP and abiotic stress better understanding of the interactions between soil
tolerance abilities based on the agro-climatic zones to variables, soil enzyme activity, functional diversity, and
empower the establishment and development of more complex bacterial communities in As-contaminated
suitable inoculants for their application in agriculture paddy soils, and provides useful information of
under local stress environments. indigenous populations with potential application to
Key words: Molecular Diversity, Salt, Rhizobacteria, Agro- improvement in soil remediation processes and explore
Climatic Zones. the deep insight of microbiological imbalance in soil
ecosystem.
SIII/P-16 Key words: Heavy metal, Paddy, Actinobacteria, Firmicutes,
Proteobacteria.
Assessment of Bacterial Community
as Fertility Indicators in Response to SIII/P-17
Heavy Metal Contamination in Paddy Characterization of Potential Microbes
Fields Soil for Arsenic Stress Amelioration in Rice
Nikita Bisht, Shashank Kumar Mishra, Swapnil Vandana Anand, Salil Kumar Arkvanshi*, Sonal
Pandey, Sankalp Misra, Vijaykant Dixit and Puneet Srivastava and Suchi Srivastava
S. Chauhan*
Division of Plant Microbes Interaction, CSIR-National
Division of Plant Microbe Interactions, CSIR-National Botanical Research Institute, Lucknow-226001, INDIA,
Botanical Research Institute, Lucknow-226001, INDIA, Email: saliltheredeemer@gmail.com, s.srivastava@nbri.res.in
Email: puneetnbri@gmail.com
Arsenic (As), a ubiquitous metalloid frequently
Heavy metal contamination especially arsenic is present in most environmental samples like soil, water,
a huge problem worldwide. Environmental issues etc. The contamination of this toxic element has become
related to heavy metal contamination draw a great a menace for the human health and natural ecosystems
concern about the soil quality of crop cultivating lands. worldwide. Millions of people are affected by the “As”
Therefore, it is essential to know the status of soil quality poisoning. Rice is popular for more efficient As
or fertility of the areas having high amount of heavy accumulation as compared to other crops due to its
metal, especially As. It is also necessary to evaluate high water requirement. Presence of As mainly in the
the impact of microbial community structure in those form of arsenite As(III) in anaerobic rice field
areas. The present work was done to examine the soil conditions and its transportation via over-expressed
physico-chemical properties, functional, and silicic acid transporters has added to the adversity.
uncultivable microbial diversity of As contaminated Microbes are known to play significant role in
paddy field soil from West Bengal, India. The result detoxification of this metalloid. Microbial activity is
revealed that the fertility indicators and growth-limiting linked to the biogeochemistry of arsenic and is involved
factors were negatively correlated with the heavy metal in its biotransformation. Several bacteria, archaea, and
stress except the soil total organic content which fungi have developed different detoxification
demonstrated significant positive correlation with the mechanisms, which include the reduction of arsenate
heavy metals. In case of functional diversity of soil, all to arsenite; oxidation of arsenite to the arsenate;
the considered diversity indices exhibited no specific immobilization, complexation, vacuolar sequestration
pattern along with the availability of heavy metals. and its methylation to less toxic organic form. The
Moreover, we observed a very complex and indifferent present study deals with the identification,
pattern of bacterial community composition in the heavy characterization and elucidation of detoxification
mechanisms of different bacterial isolates from different production of siderophore, redox system on cell
parts of India. In addition to this, the strains were also membrane, chelated ligands and acidification of nearby
characterized for different plant growth promoting traits soil by organic acid synthesis. The plant growth
viz. indole-3-acetic acid and siderophore production, promotion activity is beneficial to this approach. Thus,
and phosphate solubilization. Three strains from very small increases in bioavailable MN in edible crop
Bulandshahr, U.P., India NBRIB4.14 (Bacillus mainly staple food implement effective impact on human
tequilensis), NBRIB5.12 (Bacillus sp.) and health.
NBRIB5.12 (Lysinibacillus xylanilyticus) with Key words: Malnutrition, Micronutrient, Biofortification.
arsenate reductase, methyl transferase and arsenite
oxidase activity has been selected as best “As” tolerant SIII/P-19
strains. Assessment of arsenic stress amelioration using
rice as a host plant under greenhouse condition is under Isolation, Production and Reaction
progress.
Conditions Optimization of Amidase of
Key words: Arsenic, Abiotic stress, Methyl transferase,
Arsenite oxidase, Arsenate reductase.
Bacillus sp. MNB-1
SIII/P-20 SIII/P-21
Soybean is the important crop of Madhya production of ethylene leads to abnormal root growth,
Pradesh and largely harvested for production of this leads to impart a visible dent on plant growth and
vegetable oil. There are different types of abiotic and development. ACC synthesized in root is transported
biotic stress faced by the plants from seedling to to plant shoot where it is converted to ethylene by ACC
production stage. Drought stress adversely affects the oxidase. Bacteria being most abundant microorganisms
plant growth and yield of soybean. The present study in the rhizosphere influence the plants’ physiology to a
investigated the effect of inoculation of plant-growth greater extent, especially considering their
promoting rhizobacteria (PGPR) strains AKAD 1-2 competitiveness in root colonization, hence, referred
(Pantoea agglomerans), AKAD 1-3, (Bacillus as plant growth-promoting rhizobacteria (PGPR).
subtilis), and AKAD3-5 (Micrococcus luteus) in Several studies revealed increased health and
improving drought tolerance in soybean. These PGPR productivity of various plant species under both normal
strains were screened for different PGPR activities and stressed conditions by the application of plant
like PSB, IAA production, ammonia production, growth promoting rhizobacteria. PGPR containing ACC
catalase, chitinase, and HCN production. Effect of deaminase can lower the impact of various
PGPR inoculation on various morphological, environmental stresses such as flooding, heavy metals,
physiological, biochemical parameters was studied soil-borne phytopathogens, drought and high salt on host
under drought stress. Root colonization at the surface plant. ACC deaminase can hydrolyse the ACC,
and interiors of roots were shown using scanning intermediate precursor of ethylene to á-ketoglutaric
electron microscopy (SEM). Drought stress acid and ammonia thus inhibiting the transport to shoot
significantly affected various growth parameters, and thus retards the harmful effect of ethylene on plant.
integrity of the membrane, water status, accumulation Inoculation of ACC deaminase-containing PGPR in
of the osmolyte, which were positively ameliorated by association with plants subjected to a wide range of
PGPR-inoculation in soybean. The PGPR strain abiotic stresses results in enhanced plant tolerance
AKAD 1-2 was found to be the best in terms of against exposed stressors.
influencing biochemical and physiological status of the Key words: Plant Growth Promoting Rhizobacteria (PGPR),
seedlings under drought stress. Our report demonstrate ACC deaminase, Rhizobacteria.
the role of PGPRs (Pantoea agglomerans, Bacillus
subtilis, and Micrococcus luteus) in plant growth SIII/P-24
promotion of soybean under drought stress. The study
reports the potential of PGPR in alleviating drought Biotechnological Investigation for
stress in soybean. Propagation and Conservation of
Keyword: Soybean, Bioinoculants, PGPR, Drought stress, Beles (Opuntia ficus-indica (L.) Mill
Biofertilizer.
in Tigrey Region of Ethiopia (Africa)
SIII/P-23
Shastri Prasad Shukla 1 *, Zinabu Hailu 2 and
Applications and Mechanism of Plant Yemene Kehsay3
Growth Promoting Rhizobacteria 1
Department of Horticulture, College of Agriculture &
(PGPR) Containing ACC Deaminase Environmental Sciences (Beles Institute), Adigrat University,
Adigrat, ETHIOPIA, AFRICA; 2Research and Community
under Water logging Conditions Service, Beles Institute, Adigrat University, Adigrat,
ETHIOPIA, AFRICA; 3Beles Institute, Adigrat University,
Jyoti Prakash Adigrat, ETHIOPIA, AFRICA, Email: sp_shukla2002@
yahoo.co.in, zinisheh@gmail.com, yemane02@gmail.com
Amity Institute of Biotechnology, Amity University, Uttar
Pradesh, Lucknow Campus, Lucknow-226028, INDIA, Email: Cactus having about 130 genera and 1,500 species
jprakash@lko.amity.edu of cactaceae family. Of these, Opuntia ficus-indica
Ethylene production in plant roots is accelerated (L.) Mill. commonly known as prickly pear or Beles is
in response to both biotic and abiotic processes. Over a xerophytic, succulent, CAM (Crassulacean Acid
1
Metabolism) plant introduced in Ethiopia (Africa), Microbial Technology Division, CSIR-Central Institute of
particularly to Tigray region between 1848 and 1920. Medicinal and Aromatic Plants, Lucknow-226015, INDIA;
2
Throughout the African continents and especially in Department of Botany, University of Lucknow-226007,
Ethiopia, prickly-pear cultivation has long had an INDIA, Email: biosuman14@gmail.com
important role both in subsistence agriculture and in Growing demand for mint oil in food and flavours
the intensive fruit production. Beles is a multipurpose requires eco-friendly agricultural practices. Plant
plant with great potential as source of livestock feed in growth promoting rhizobacteria or fungi alone or in
Tigray region and Ethiopia at large. It is known as a combination become useful soil microorganisms
multipurpose plant since it can be used for human food improving production and control abiotic and biotic
(fruits and vegetables), medicinal and ornamental plants, stresses. The present study was conducted to assess
animal forage, natural wind break barrier, soil stabilizer, the potential of co-inoculation of Trichoderma spp. with
re-vegetation resource to control water and wind erosion the plant growth promoting rhizobacteria (PGPR) on
in eroded soils. The sweet fruit of this plant is known the growth and essential oil yield of Mentha arvensis
as “Tuna” useful as human food and entire plant can cv. “Kosi” under pot as well as field conditions. The
be cultured as raw-industrial material to produce several results revealed that dual inoculation of Trichoderma
sub-products such as jam, wine, candies and jellies etc. spp. and Brevibacterium spp. increased plant growth,
It is also source of natural-dye, carminic acid which is oil content, leaf-stem ratio, photosynthetic pigments,
used for colouring fabrics, food, and cosmetics. Stem and nutrient uptake resulting in higher oil yields. The
of this plant has been reported to treat diabetes and density (colony forming unit) of Trichoderma, as well
useful in the cure of hyperlipidemy (excess of lipids in as rhizobacteria, was significantly higher when
the blood) and obesity. O. ficus-indica is well compared to the single inoculations and control.
recognized for their wound healing properties and Scanning electron microscopy revealed significant
anticancer effects. Conventionally, O. ficus-indica is colonization of microbes on the root surface particularly
propagated by seeds but physiological limitations of the for the best combination of Trichoderma and
seeds, such as; low germination rate, genetic Brevibacterium spp. Similarly, under field conditions
segregation, less guarantee of genetic stability, a long the synergistic association of Brevibacterium spp. and
juvenile stage, less availability and low viability, and Trichoderma showed increased plant growth which
slow seedling growth rate are major constraints in the provides evidence that both enhance the growth
production of the population of this species in the nature. promoting abilities of each other significantly improving
Therefore crop improvement programme, microcloning growth and yield of the crop. A higher number of
and ex-situ conservation of the species is underway propagules, both of Brevibacterium spp. and
which is a principal objective of the Beles Institute of Trichoderma may exert similar crop growth benefits
Adigrat University, Adigrat, Ethiopia (Africa). in the subsequent crops while higher the population of
Key words: Opuntia ficus-indica, Biodiversity, Trichoderma may protect the subsequent crops from
Conservation, Micropropagation, CAM plant. many phytopathogens.
Key words: Mentha arvensis, Trichoderma spp., Plant
SIII/P-25 growth promoting rhizobacteria, Brevibacterium spp.,
Scanning Electron Microscopy.
Evaluation of Selected Compatible
Microbial Consortia for the Growth SIII/P-26
Promotion and Increased Essential Oil Algal Biomass Pretreatment for
Content in Mentha arvensis under Enhanced Methane Potential
Field Conditions
Shashi Bhushan1*, Mohit Singh Rana2 , Mamta1
Suman Singh 1 *, Arpita Tripathi 1 , Deepamala and Sanjeev Kumar Prajapati1
Maji1, Anju Yadav1, Poornima Vajpayee2 and Alok 1
Bio-Resource Engineering Lab (BREL), Department of
Kalra1 Chemical and Biochemical Engineering, Indian Institute of
Technology (IIT) Patna, Bihta, Patna-801106, Bihar, INDIA; Abiotic stresses are one of the major barriers for
2
Department of Energy and Environment, National Institute crop productivity. The native rhizospheric soil
of Technology (NIT) Trichy, Tiruchirappalli-620015, Tamil microflora has limitations to withstand against these
Nadu, INDIA, Email: shashiascientist@gmail.com abiotic stresses and is mostly exclusive to plant growth
Human kind receives their major portion of energy promotion. In the current study, an attempt was made
needs from fossil fuels. These fuels are non-renewable to evaluate soil bacteria from extreme environments
and their reservoir is finite. Ever increase energy of volcano soils based on plant growth promoting and
demands can be addressed through biofuels. Among abiotic stress mitigating characteristics. The screening
the available biofuel substrate, algal biomass shows led to 08 (NBRISH4, NBRISH6, NBRISH10,
most promising feed stock for biodiesel, biomethane, NBRISH11, NBRISH13, NBRISH14, NBRISH16 and
and bioethanol. Microalgae contain chlorophyll and NBRISH26) bacterial isolates capable of withstanding
perform photosynthesis at higher rate as compared to stress namely temperature (up to 45° C), salt (up to
the terrestrial plant. They can trap atmospheric carbon 2M NaCl) and drought (up to 60% PEG) in vitro.
dioxide and fixed them in biomass. Their cell wall is Further, the selected isolates were outstanding in vitro
composed of cellulose, which make them tuff and temporal performance with regards to their survival
resistant to many cell disruption techniques. To enhance (in terms of colony count), auxin production, phosphate
the biofuel potential of the algal biomass, it is advisable solubilization, biofilm formation, alginate and exo-
to perform pretreatment steps. These pretreatment polysaccharide production abilities under abiotic stress
steps enhance the anaerobic digestibility of cell wall (40°C temperature; 500 mMNaCl representing salt;
and release the high value cellular content. This paper 250 mM LiCl representing drought) conditions. In vivo
gives a brief idea about the available pretreatment treatments of each bacterium to maize plants resulted
modules. It also present the results of physical into significant enhancement in root and shoot length,
pretreatment (microwave, ultrasonic and thermal) root and shoot fresh and dry weight and no. of leaves
performed on Chlorella pyrenoidosa. Among tested per plant (NLPP). Overall, the plant growth promoting
technique microwave seems most efficient in terms of and abiotic stress tolerance ability was prominent for
soluble COD (Chemical oxygen demand) recovery. The NBRISH6 bacterial isolate which was identified as
released COD enhance the methane potential of algal Ochromobactrum sp. using 16S rRNA based
feedstock. phylogenetic analysis. Therefore, NBRISH6 having
multiple plant beneficial and abiotic stress tolerance
Key words: Microalgae, Biofuel, Pretreatment, COD,
Methane. characteristics shall be considered for its application in
agriculture under stress environments.
SIII/P-27 Key words: Rhizobacteria, Ochromobactrum, Abiotic
stresses.
Characterization of Plant Growth
Promoting Rhizobacteria Isolated SIII/P-28
Scientific and Innovative Research (AcSIR), Anusandhan silencing and overexpression analysis among japonica
Bhawan, 2 Rafi Marg, New Delhi-110001, INDIA, Email: and indica rice subspecies. Further phenotypic analysis
yuvi_indolia@yahoo.com of OsSERK and OsGLK overexpressing lines of PB-1
Rice (Oryza sativa L.), a major cereal crop for variety (Indica subspecies) showed significant
around half of the world population, also acts as a model increase in somatic embryogenesis and regeneration
crop for monocot plants in order to study various potential compared to wild type. On the other hand,
developmental, environmental and biochemical aspects knock down lines of Nipponbare variety (Japonica
due to its small size genome (430 Mb), better genomic subspecies) showed notable decrease in somatic
identity with other cereal crops and comparatively better embryogenesis and regeneration efficiency compared
transformation system using agrobacterium mediated to wild type. Functional significance of both the genes,
transformation. Compare to japonica, indica rice has in terms of in vitro plant regeneration may be used as
been less responsive to in vitro culture. In the present an efficient tool for improving agrobacterium mediated
study, comparative somatic embryogenesis and transformation, especially in cereal plants.
regeneration efficiency of two genes (OsSERK and Key words: Rice, Somatic embyrogenesis, Japonica, Indica.
OsGLK) was carried out using amiRNA mediated gene
Session IV
Environmental Impact Assessment
the CRA approach. This involved scoring of five major basis. Water quality of the Ghat has been assessed on
attributes/aspects of species invasion through direct a regular basis during the summer, winter and rainy
measurements in the field, deductions from field season, in the course of the present study. Parameters
observations, and inferences based on information like turbidity, temperature, pH and EC were measures
available in the literature. The attributes were (i) species on spot by thermometric and potentiometric method,
morphological features, (ii) biological characteristics & respectively. Water samples were collected in BOD
dispersal ability, (iii) distribution potential, (iv)ecological bottles as well as pearl pet bottles for investigating
impact, and (v) difficulty to control. The study revealed various parameters including Alkalinity, Hardness,
that as more risk of invasion has been observed for Chlorides, Nitrate, total dissolved solids, BOD (5-day),
Eichhornia crassipes, Ipomoea carnea, Phosphate etc. National Environmental Engineering
Alternanthera philoxeroides, Ipomoea aquatica, Research Institute and the American Public Health
Lemna minor, Pistia stratiotes, and Trapa natans, Association protocols were followed for investigating
these species should be prioritized for suitable remedial various physic-chemical parameters. Significant
measures to control their further spread in the study seasonal variation has been observed in the physic-
area. The present study therefore showed that through chemical characteristics of the study site. These
CRA approach it is possible to identify the AMs with variations were also very obvious in accordance with
greater invasion potential which in turn would help the the Hindu festive calendar. An increase in values of
scientists and policy makers in prioritizing such species EC, pH, TDS, turbidity, alkalinity, chloride, BOD,
for their control and management in a suitable way. phosphate etc. have been recorded particularly during
Key words: Invasion potential, Scoring technique, Aquatic the winter months and after Kartik and Maagh months
macrophytes, Tropical floodplains, Barak basin. of the Hindu calendar. Same is true for the period
following the immersion of idols after the two Navratras
SIV/O-3 and Ganesh festival.
Key words: Jaipur Ghat, Physico-chemical analysis, Pollution,
Effect of Religious Rituals on the Pushkar Sarovar, Religious Rites, Seasonal variations.
Water Quality of Jaipur Ghat of
SIV/O-4
Pushkar Sarovar (Rajasthan)
Assessment of Soil Pollution using
Anita Sharma
Multivariate Techniques and Indexing
Samrat Prithviraj Chouhan Government College, Ajmer,
Rajasthan, INDIA, Email: anitasharma9339@gmail.com Approach
The Pushkar Sarovar, situated in the lap of the Vinod Kumar
Central Aravalli Ranges in the State of Rajasthan, is
perhaps one of the most revered holy lake. It is Department of Botany, DAV University, Jalandhar-144012,
INDIA, Email: vinodverma507@gmail.com
supposed to have originated at the point where Lord
Brahma’s lotus struck the land. Thousands of devotees Soil pollution is one of the most important issues
visit the Sarovar to take holy bath and worship it. Over throughout the globe and increasing urbanization,
the years, this resulted in enhancement of pollution level industrialization and application of pesticides, fertilizers
in the water body accompanied with accelerated etc. on agricultural activities are responsible for soil
eutrophication. Steps have been taken by the local pollution. The present work was designed to study the
administration and various local groups to combat the soil properties (pH, conductivity, soil organic matter,
problem. One of the common decision taken was not phosphorus, calcium and magnesium) and heavy metals
to immerse idols, flowers and ashes into the Lake. A (Cr, Cu, Pb and Co) in soils of Jalandhar District. Cluster
part of the lake has been delimited at the Jaipur Ghat analysis (CA) and principal component analysis (PCA)
for the purpose. The concrete tank built for the purpose showed that anthropogenic activities have great
is cleaned of the debris, thus preventing the Lake from influence on the soil properties and heavy metal content
receiving the pollutants directly and that too on a regular in the soil. The various indices such as contamination
factor, geoaccumulation index, potential contamination this species under both current and future climate
index, enrichment factor (%), pollution index and change scenarios. Also, the Himalayas is predicted to
potential ecological index showed that soils are be at moderate invasion risk under climate change. The
moderately polluted by the heavy metals. This is the research findings of the present study have practical
first such baseline study in the area and further research applications in predicting invasion hotspots and
needed in order to monitor the soil pollution status of developing region-specific management strategies in
Jalandhar, District. the country.
Key words: Heavy metals, Soil pollution, Jalandhar. Key words: Biogeographic zones, Climate change,
Distributional modelling, Habitat suitability, Invasion.
SIV/O-5
SIV/O-6
Potential Distribution and Invasion
Risk Assessment of Parthenium ICT Promotes Environmental
hysterophorus in India under Climate Awareness through ENVIS activities
Change under a National Programs
Sunil Tripathi*, Diwakar Saini, Vineeta Yadav,
Rameez Ahmad 1 *, Anzar A. Khuroo 1 , Maroof
Amrita Awasthi and Pankaj Kumar Srivastava
Hamid1, Bipin Charles2 and Zubair A. Rather1
1 Environmental Technologies, CSIR-National Botanical
Centre for Biodiversity and Taxonomy, Department of
Research Institute, Rana Pratap Marg, Lucknow-226001,
Botany, University of Kashmir, Srinagar-190006, J&K, INDIA;
2 INDIA, Email: sunil9351@gmail.com
Ashoka Trust for Research in Ecology and the Environment
(ATREE), Royal Enclave, Srirampura, Jakkur PO, Bengaluru- Environmental information plays a vital role not
560064, INDIA, Email: rameezkhuroo929@gmail.com only in formulating environmental management policies,
Congress grass (Parthenium hysterophorus L.), but also in the decision making process aiming at
native to Tropical America, is one of the world’s worst environmental protection and improvement of the
weeds with deleterious ecological and economic environment for sustaining a good quality of life of the
impacts. In India, the weed was accidentally introduced living beings. Realizing this need of information system,
from Central America about a century ago and since Ministry set up an Environmental Information System
then it has invaded most of the regions across the (ENVIS) in 1983 to establish a comprehensive network
country. The present study using ensemble modelling in environmental information collection, collation,
approach implemented in biomod2 package has storage, retrieval and dissemination to users. Presently,
mapped the current and future distribution of P. The ENVIS network consists of 66 network partners,
hysterophorus in India and assessed its invasion risk out of which 35 are subject-specific and 31 State/UT.
in different biogeographic zones under climate change These network partners are called ENVIS Centers that
scenarios. The modelling results revealed that about are located in the notable organizations/institutions/State/
65% of the total area in the country is suitable for UT Government Departments/Universities throughout
potential invasion of this species under current climatic the country. ENVIS centre at CSIR-National Botanical
conditions. The model predicted a decrease in overall Research Institute has started in January 2004 and
habitat suitability for this species under future climate focusing on the specific theme of “Plants and Pollution”.
change with more than half of suitable habitat reduced ENVIS Resource Partner-NBRI systematically
under RCP 8.5-2070. Nonetheless, some of the collects, compiles and disseminates data on all the
currently invaded areas will become highly vulnerable diverse aspects of “Plants and Pollution” viz; effects
to invasion under climate change. On downscaling to of different pollutants on plant kingdom and plant
different biogeographic zones, North-east and coastal resources for mitigation ofspecific pollutants. The centre
areas in the country remained as high and moderate is publishing theme based Newsletters (Quarterly),
risk regions respectively to the potential invasion of Subjective News (monthly), thematic bulletins (twice
a month), maintaining Databases (34), Case Studies, is resistant or to cope with open cast mining generated
Latest news based on theme, Upcoming Events, dust pollution in and around mining areas as well as
Glossary, Bibliography, technical reports, etc. The beautification of adjacent highways. Maximum
ENVIS RP-NBRI website is designed and fully devoted deposition was recorded in Ficus hispida and followed
on the latest information about the specific theme by Calotropis procera, Butea monosperma, Ficus
“Plants and Pollution” and it helps to the scientists, benghalensis, Allianthus excelsa, Azadirachta
researches, students etc. Those are involved in the indica.
research area of the same. Besides these, ENVIS also Key words: Dust pollution, Chlorophyll content, Ficus spp.,
provides Discussion Forum and a mobile app viz; National highway.
“Green Planner” (Android and itunes app). Further,
ENVIS centre is do various outreach activities to spread SIV/O-8
environmental awareness among the people. The
national and international scientific data present on Determination of Geochemical
ENVIS RP-NBRI website are of immensely use to Properties and Temporal Variation of
several research organizations, town planners, policy
makers, teachers, students including kids and others Ground Water in Kanchipuram
stakeholders to aware about Environmental status District, Tamilnadu, India
especially related to the plants and pollution.
Key words: ENVIS, Environmental Information System, A. Chandrasekaran1* and R. Ravisankar2
Plants and pollution, Pollutants, Green planner. 1
Department of Physics, SSN College of Engineering,
Kalavakkam, Chennai-603110, Tamilnadu, INDIA;
SIV/O-7 2
Department of Physics, Government Arts College,
Tiruvanamalai-606603, Tamilnadu, INDIA, Email:
Open Cast Mining and Impact of Dust chandrasekarana@ssn.edu.in
on Roadside Plant Species at Jhansi- Water is essential for all living organisms for their
Allhabad National Highway existence and metabolic process. The aim of the present
study is to assess the quality and temporal variations
Priyanka Singh and Amit Pal* of ground water in Kanchipuram district, Tamilnadu,
India. In order to assess the water quality, water
Institute of Environment & Development Studies,
samples were collected from 20 different locations in
Bundelkhand University, Jhansi-284128, INDIA, Email:
apu13@rediffmail.com, spriyanka680@gmail.com
pre-monsoon season and geo-chemical properties such
as pH, Total Dissolved Solids (TDS), Electrical
Deposition of dust on roadside plant and its impact conductivity (EC) Chloride (Cl-), Sulfate (SO4 2-),
on leaf i.e. leaf pigment concentration, the carotenoid Calcium (Ca2+), Magnesium (Mg2+), Nitrate (NO3-),
and protein has been investigated on fifteen Sodium (Na +), Potassium (K+), Carbonate (CO3),
selectedroadside plants species namely Allianthus Hydrogen Carbonate (HCO3-) were determined using
excelsa, Azadirachta indica, Butea monosperma, different analytical methods. The measured geo-
Calotropis procera, Cassia fistula, Datura metel, chemical properties are compared with prescribed limits
Ficus benghalensis, Ficus hispida, Ficus infectoria, given by world health organisation (WHO, 2004). The
Ficus religiosa, Holoptela integrifolia, Millettia results of geochemical properties indicates that average
pinnata, Nerium oleander, Phoenix dactylifera and of Ca, Cl, NO3 and pH slightly higher than WHO, 2004
Psidium guajava respectivelyat Jhansi-Allahabad permissible limit whereas other measured properties
National Highway – 76. The variation in terms of dust are lower. Multivariate statistical techniques like
deposition with species specific result observed during Pearson correlation, factor and cluster analysis were
the entire period of study. Declination of leaf pigment applied to water quality variable for evaluation of
concentration, carotenoid content and protein content temporal variations in the study area.
indicate the positive impact of dust pollution. Above
Key words: Ground water, Temporal variations, Multivariate
findings may be helpful to find out some species which
statistical techniques.
may arise due to the proposed residential project. current study reveals that the trend of organochlorine
Environment Management Plan is a practical and pesticides is decreasing in comparison to the past. The
achievable plan of management to ensure that any study concluded that the levels of metals and pesticide
environmental impact during all the phases is minimized residues are variable in different fish species but within
and lead in the direction of sustainable development. the maximum residue levels recommended at National
EMP for the above mentioned impacts are; provide and International standards.
separate entry & exit points and driveways, site Key words: Accumulation, Fish species, Heavy metals,
barricading, installed organic waste converter, frequent Pesticides
water sprinkling. In conclusion, results from EIA study
showed that the proposed residential development SIV/P-5
project has significant impacts on the environment.
Implementation of an effective EMP will minimize the Tree Falling Risk Assessment of IOCL
environmental issues occur during the project cycle. Campus New Bongaigaon, Assam and
Key words: Building, Construction, Environment, EIA, Impact. their Control Measures
SIV/P-4 Rehanuddin, Priyanka Agnihotri, P.S. Chauhan and
S.K. Tewari
Heavy Metals (Pb, Cd, Hg) and Org- CSIR-National Botanical Research Institute, Lucknow-
anochlorine Pesticides in Freshwater 226001, INDIA, Email: rehanfaruqui2117@gmail.com
Fishes: Risk Evaluation for Consumers Trees are important natural resources; they
provide us food, cloth, medicine, firewood, timber wood,
Geeta Singh beauty and shade also. An overall assessment of trees
present near at IOCL sites in Bongaigaon, Assam has
DDU Gorakhpur University, Gorakhpur-273009, Uttar
been done in order to identify the risk of falling
Pradesh, INDIA, Email: geetasanger@gmail.com
associated with them. In order to carry out the study,
Fishes are a valuable source of high-grade protein grouping of trees depending on their position, angle of
and nutritious component of the human diet. Heavy inclination and ageing were done. Few of them were
metals and OCPs from natural and anthropogenic found to be infected with different microbes, which
sources are released into aquatic ecosystems, where weakens them and makes them prone to fall. Several
they pose a serious threat because of toxicity, long approaches have been made to manage the risks from
persistence, bioaccumulation, and biomagnifications in trees which involved assessment of trees according to
the food chain. Fishes are badly affected because they the risk of their falling and causing injury, and further
are top consumers in aquatic life. Five most cutting of vulnerable trees alleviating the danger. Total
commercially important fresh water fish species were 72 trees are observed/studied at the study site, some
collected, digested and analyzed for organochlorine of which pose direct threat on pipeline of IOCL which
pesticide and toxic metals. The metal concentrations probably leads to bursting of the pipeline causing several
ranges of Pb, Cd and Hg detected were 0.02-18.0, hazards. Therefore “Tree Falling Risk Assessment”
0.05- 21.0 and 0.01-26.0 ppb respectively. Among the has been undertaken emphasizing the prevention of any
three metals, concentration of mercury was detected hazard. The parameters under consideration involve
highest. The fish samples were contaminated with one girth of the trees, their height, angle of inclination,
or more DDT analogue and HCH isomer residues number of trees infected by pests, termites, pathogen
respectively. pp-DDT and gamma-HCH was the most followed by their age. Biological treatment (Basically
frequently occurring residues in fish samples. The level Trichoderma and PSB) has been provided to eradicate
of contamination with pp-DDT ranged ND-48.0 ppb infection, while the trees carrying high potential of
and gamma-HCH from ND-68.2 ppb. Delta-HCH damage due to major degree of infection need to be
isomer was not detected in any of the fish sample cut for the safety purposes.
analyzed. Concentration of total DDT is higher than Key words: Trichoderma, PSB, Tree Falling Risk Assessment,
total HCH. The level of pesticides observed in the IOCL.
Session V
Sub-tropical and Tropical Oak under
Global Change
SV/KN-1 SV/KN-2
Genetic Diversity, Hybridization and Prem Prakash Singh*1 , Anna Dermann2, Florian
Dermann 2 , Dibeyndu Adhikari 1 , Tamalika
Conservation of Mexican Oaks Chakraborty3, Purna B. Gurung 1 , Saroj Kanta
(Fagaceae: Quercus) Barik1,4, Jürgen Bauhus2 and Somidh Saha2,5
1
Department of Botany, North-Eastern Hill University, INDIA,
Ken Oyama* 2
Chair of Silviculture, University of Freiburg, Freiburg,
Universidad Nacional Autónoma de México (UNAM), GERMANY, 3Chair of Site Classification and Vegetation
National Autonomous University of Mexico, MEXICO, Email: Science, University of Freiburg, GERMANY, 4National
kenoyama@enesmorelia.unam.mx Botanical Research Institute, Lucknow, INDIA, 5Institute for
Technology Assessment and Systems Analysis, Karlsruhe
Mexican oaks (Fagaceae: Quercus) are one of Institute of Technology, Karlsruhe, GERMANY, Email:
the richest genera of trees with more than 160 species prem12flyhigh@gmail.com
described including 109 endemics. Mexico is considered
The montane subtropical broadleaved wet hill
one of the main centres of oak diversification in the
forests of Meghalaya are part of megadiverse biome.
world. Oaks are distributed in temperate and subtropical
It is situated at the transition zones between the
forests in heterogeneous environments along an
Himalayas and Indo-Burma biodiversity hotspots. Ten
altitudinal gradient of 0 to more than 2500 m asl. Oaks
tree species of Fagaceae family dominate these forests.
are considered key species in the ecosystems where
Fagaceae tree species are important for sustenance
they are distributed with an intricate net of interactions
of the local tribal populations. These forests are highly
with ectomycorrhizal fungi, insects, epiphytes, among
degraded and fragmented due to anthropogenic
others. A comparative analysis of population genetic
disturbances such as mining, unsustainable forest
diversity between selected oak species distributed in
utilization, shifting cultivation, and browsing. In past,
Mexico and other parts of the world indicated that
these forests originally formed large continuous areas
Mexican oaks harbor higher levels of genetic diversity
in the landscape. Today, numerous degraded and
but low genetic differentiation. Hybridization between
fragmented forest patches are scattered over the entire
different oak species can explain in part this high levels
landscape. In this study, we assessed for the first time,
of genetic diversity. Patterns of oak hybridization are
the restoration potential i.e. capacity of natural
presented in order to show the different modes and
regeneration and state of vitality of Fagaceae species
number of oak species involved. Conservation of oak
in Meghalaya. The main objective was to understand
forests in Mexico is urgent due to the high deforestation
how different biotic factors, abiotic factors, and
rates and the fragmentation of remnant natural forests.
anthropogenic disturbances influence restoration
Key words: Genetic differentiation, Mexican Oak, Population potential of Fagaceae species. We selected two
genetic diversity. Lithocarpus, four Castinoopsis, four Quercus species
and fragmented forest patches in six locations on an
elevational gradient between 800 - 2000m asl along
the south-facing slope of Khasi Hills, Meghalaya. We
found the degraded forests still harbour high tree species
richness (143 species). Additionally, our study showed
that the plant community was heterogeneous in
composition, structure, and distribution. We found a
general trend that the Fagaceae family had high natural
regeneration potential i.e. a high number of seedlings entropy (MaxEnt) model using field observations and
and saplings but low recruitment of adult trees. The literature generated occurrence locations and CMIP5
vitality of Fagaceae species was poorer than non- (Couple Model Inter-comparison Project) derived
Fagaceae species because of high anthropogenic bioclimatic variables were used for the study. The
disturbance to Fagaceae species. This case study will predictions were done on the geographic area of
help us in enhancing our understanding of the status of Uttarakhand according to four IPCC RCPs for the
these forests and possible restoration strategies for these future periods 2050 and 2070. Our results show that
degraded and fragmented forests patches of the estimated potential area of the species is likely to
Meghalaya. have mixed results in future for all climate change
Key words: Forest Landscape Restoration, Quercus, scenarios for Quercus in its actual habitats. Shifts in
Castanopsis, Lithocarpus, Forest, Regeneration, geographic distributions of climatic niches reflect
Anthropogenic Disturbances. unusual patterns, implying the need for climate adaptive
habitat restoration. Solutions intended need to be
SV/O-2 climate resilient restoration of degraded patches using
in-situ conservation measures.
Assessing the Impacts of Climate Keywords: Oak forests, Central Himalayas, Uttarakhand,
Vulnerability on Quercus Habitats in Climate Change, Species Distribution Models
Central Himalayas: Future SV/O-3
Implications
Status of Oaks and Related Species
Shalini Dhyani* and Rakesh Kadaverugu for Management and Conservation
CSIR-National Environmental Engineering Research
Institute, Nagpur-440020, Maharashtra, INDIA, Email:
Strategies
s_dhyani@neeri.res.in
Yangchenla Bhutia1,2*
Oak forests in Central Himalayas represent 1
Suri Sehgal Centre for Biodiversity and Conservation,
climax between 1000-3500 m amsl. Five species of Ashoka Trust for Research in Ecology and the Environment,
oaks viz. Quercus glauca, Q. leucotrichophora, Q. Jakkur Post, Royal Enclave, Sriramapura, Bangalore-560064;
floribunda, Q. lanata and Q. semecarpifolia grow Karnataka, INDIA; 2Manipal Academy of Higher Education
naturally and successively replace other species along (MAHE), Manipal-576104, INDIA, Email:
the increasing elevation in stated order. Oak dominated yangchenla.bhutia@atree.org
mixed broadleaved forests not only provide efficient
The oaks and its related tree species form the
provisioning ecosystem services but are also source of
dominant composition of temperate forests. They
regulating, supporting and cultural ecosystem services
contribute significantly to overall functioning of forest
in the region. Oak forests are proven for their high
ecosystem. Besides, they also have an intricate history
water holding capacity, sustaining healthy watersheds
with humans for its different uses. But, these species
and spring sheds. Deforestation in last few decades
are globally threatened, and the Himalayan oaks are
has led to change in succession patterns and biodiversity
no exception. Recent studies from the Eastern
loss due to accelerated invasion of Chir Pine (Pinus
Himalayan region of Sikkim reported forest degradation
roxburghii) in oak forests. Cumulative impact of human
particularly concentrated in the oak forests. In the
interferences coupled with climate change has been
current study we evaluated the diversity, structure,
significant on oak dominated forests. Conservation of
forest composition of tree species, including the
oak forests is a socio-ecological challenge that requires
regeneration status of oaks and their related species
an interdisciplinary approach. The present study
along the altitudinal gradient. We covered three forest
focuses on assessing the climatic niche of Quercus
types ranging from 900m to 3200m above sea level,
species and understanding its shift in the future
the potential distributions zone of oak and its related
distributions due to climate change. The Maximum
species. Our result reported oaks and its related species
as the major contributor to the overall forest ecosystem over-exploitation and growth of invasive species. About
with highest Importance Index Value. However, an 78 species of oaks are listed as threatened. Himalayan
exhaustive analysis showed a more nuanced condition. oak forests are under serious threat and are being
One of the major concerns was the size class invaded by pine forests due to the increase in
distribution. Overall the oaks and the related species temperature. The species growing as associates of pine
were predominantly of large diameter. However, the may cross the frontiers of pine forests, and become
low elevation forests completely lacked trees above new elements of the oak forests. In most of the oak
80cm diameter. It is feared under current situation the species, seed (acorn) germination and seedling survival
oak forests will disappear with unsustainable loss. We are very poor. It is reported that only one in 10,000
suggest conservation strategies focusing on of different acorns results in an eventual tree.This critical issue
stages of plant growth to ensure perpetual functioning prompted us to study the seed germination, seedling
of forest ecosystem. survival and growth in two species of Quercus (Q.
Key words: Forest composition, Conservation strategies, dealbata and Q. griffithii) growing in northeast India.
Regeneration status. Seeds of these two species vary widely in mass and
can be categorized into light-, medium- and heavy-
weight classes. Protein, carbohydrate, lipid and energy
contents were greater in heavy seeds than in the
SV/O-4
medium-weight and small seeds. The heavy seeds
emerged earlier and showed higher germination
Effect of Microsite Characteristics on percentage than small seeds. Further, the seedlings
Seed Germination, and Survival and emerged from heavy seeds performed better in terms
Growth of Seedlings of Two Species of survival and growth. Of the four substratum
microsites tested (T 1 - herbaceous vegetation was
of Quercus Growing on the Hills of allowed to grow and litter was left undisturbed; T2 –
Northeast India herbaceous vegetation was allowed to grow, but litter
was removed; T3 - herbaceous vegetation was cleared
M.L. Khan1* and R.S. Tripathi2 but litter was left intact; T4 – both litter and herbaceous
1
Department of Botany, Dr. Harisingh Gour Vishwavidyalaya vegetation were removed), emergence, survival and
(A Central University), Sagar-70003, MP, INDIA; 2Ex-INSA growth of seedlings were highest in the T4 plots and
Senior Scientist, CSIR - National Botanical Research Institute, lowest in T1 plots. Further, seedling emergence, survival
Rana Pratap Marg, Lucknow-226001, INDIA, Email: and growth were more adversely affected by the
khanml61@gmail.com herbaceous cover than the litter. The occurrence of
The genus Quercus (oak) is native to the northern seeds of different weights showing large variation in
hemisphere and extends from cold latitudes to tropical food reserves and energy content in two studied oak
Asia and the Americas. Oaks are considered as species, and differential response of these seeds and
keystone species in a wide range of habitats from seedlings produced by them to microsite characteristics
Mediterranean semi-desert to subtropical rainforest. may be viewed as an important regenerative strategy.
Quercus species are considered humus-creating The findings of this study have considerable implication
machines, house for many animals, source of free for the silviculture and management of oak forests.
livestock feed and creator of microclimates. However, Key words: Quercus spp., Seed mass variability, Seed
many species of oaks are under threat of extinction in germination, Seedling survival and growth, Microsite
the wild due to destruction of their natural habitats, characteristics.
in the literature in respect of a few species. Future assessed on the mortality of the trees. Climate-growth
distributional ranges as predicted under different climate relations (fifty years), resistance, recovery, and
change scenarios show that some species will lose resilience (for multiple droughts) were quantified. The
climatic suitability, while in case of some other species, retrospective ecophysiological analysis was performed
previously less suitable areas will become more by using stable C and O isotopic signatures of the tree
conducive for species persistence. However, certain rings. We found, increasing AWC, light, plant-plant
areas will retain climatic suitability. We suggest that interactions, and tree species diversity reduced
such areas may be useful for long-term conservation mortality. Beech trees died when the dead
of oaks. Appropriate silvicultural practices should be aboveground-biomass crossed a threshold of 58%. The
developed in these areas to counter the climate and dependency of tree growth to AWC became higher
anthropogenic pressure. with increasing magnitude of drought. Recovery and
Key words: Fagaceae, Ecological niche, Climate change, resilience were higher in trees with higher AWC, but,
Geographic distribution. opposite for resistance. Dual isotopic analyses revealed
that beech trees growing in lower AWC plots showed
SV/O-7 a higher response to drought, climatic dependency, and
higher stomatal resistance compared to those growing
Temperate Broadleaved European in higher AWC; later showed higher stomatal
Beech (Fagus sylvatica L.) Trees are conductance. We conclude that: 1) beech trees growing
on dry sites have a high ecological plasticity to drought;
Showing High Ecological Plasticity however, died after crossing a threshold of dead
under Multiple Drought Events aboveground-biomass; 2) high tree species diversity in
dry sites could mitigate drought stress in beech trees;
Tamalika Chakraborty1*, Somidh Saha2,3, Andreas and 3) changes in soil water condition even at microsite
Matzarakis4 and Albert Reif1 level could alter the growth and survival strategies of
1 beech trees. This study will enrich the state of the
Chair of Site Classification and Vegetation Science, Faculty
of Environment and Natural Resources, University of knowledge about the ongoing debate on the vulnerability
Freiburg, Tennenbacherstr. 4, D-79106 Freiburg, GERMANY; of beech trees to drought in Europe.
2
Institute for Technology Assessment and Systems Analysis, Keywords: Available Soil Water Storage Capacity, Biomass,
Karlsruhe Institute of Technology, Karlstr. 11, D-76133 C and O Stable Isotopes in Tree Rings, Dendroecology, Plant-
Karlsruhe, GERMANY; 3Chair of Silviculture, Faculty of plant interactions, Tree mortality.
Environment and Natural Resources, University of Freiburg,
Tennenbacherstr. 4, D-79106 Freiburg, GERMANY; 4Centre SV/O-8
for Medical Meteorological Research, German Weather
Service, Stefan-Meier-str. 4, D-79104 Freiburg, GERMANY, Mapping of Ecosystem Services as
Email: tamalika.chakraborty@waldbau.uni-freiburg.de,
chakraborty.tamalika@gmail.com Sustainable Management Tool – A
Droughts in central Europe have been increasing Case from Oak Forests in Western
due to rising temperature and shifting precipitation. We Himalayas, India
studied the cause and threshold of drought-driven
mortality and survival strategies of beech trees, which Sabyasachi Dasgupta1,2 *, Subhasis Mahato1 and
is the most important broadleaved tree species of central N.P.Todaria1
Europe. The study was conducted in five near-natural 1
Department of Forestry and NR, H.N.B. Garhwal University,
dry forests in Germany and Switzerland. Tree-level Srinagar (Garhwal), Uttarakhand-246174, INDIA; 2
mortality (as a percentage of dead aboveground- Department of Forestry and Biodiversity, Tripura University,
biomass) and available soil water storage capacity Agartala, Tripura-799022, INDIA, Email: sdhnbgu@
(AWC) were quantified. Influences of biotic (plant- gmail.com
plant interactions, tree species, structural diversity) and
Through out the world oak species have
abiotic (light, AWC, soil pH, bulk density) factors were
considerable economic and conservation significance.
However, the Garhwal Himalayan Oak forests provide Institute of Technology (KIT), Karlsruhe, GERMANY;
2
various ecosystem services and are not considered for Institute of Geography and Geoecology (IfGG), Karlsruhe
their timber value. The present study was desired to Institute of Technology (KIT), Karlsruhe, GERMANY,
3
explore the spatial mismatch in the delivery of Hooman Latifi, Khajeh Nasir University, Tehran, IRAN,
Email: e.shafeian90@gmail.com, fabian.fassnacht@kit.edu,
ecosystem services by oak forest ecosystems and also
Hooman.Latifi@kntu.ac.ir
to understand the dynamics and future of oak in the
Himalayas. An oak dominated forest site was selected An alarming decrease in oak forest habitats has
in Rudraprayag district of Uttarakhand, India with an been recently reported throughout the world. Oak
average elevation of 2400m above sea level. The stock decline has also been observed in the Zagros mountain
and flow data were mapped using polygon approach area in Iran. Identifying and mapping declining trees is
with the help of GIS techniques. Contribution of different an important step to manage the spread of infestations.
species to total forest biomass significantly varied. The Several earlier studies have demonstrated the potential
biomass extraction was disproportionate to availablility of remote sensing for such purposes, however, there
of Quercus semecarpifolia and Quercus are still open questions concerning why certain declining
leucotrichophora in that particular forest. Flow of areas can be detected with remote sensing and others
ecosystem services in the form of fuel wood and green not. Moreover, mapping declined areas is just the first
fodder was variable in spatio-temporal scale. In flow step towards understanding the reasons for forest
and stock map, it was clearly visible that the flow of decline. Once the affected areas have been identified,
fuel was more in lower altitudinal zone whereas fodder it is necessary to understand the underlying drivers that
flow was high in the mid and high altitudinal zones. have caused the decline. In this paper, we will review
The availability of fodder was less near the village area the current literature on oak dieback, with a specific
because of more fuel and fodder extraction in the past. focus on Iranian Oak forests and the current status on
Therefore, if extraction is not controlled, the mid and the application of remote sensing data to understand
upper elevation oak forest will be soon degraded. Spatial and map patterns and processes related to oak decline.
dynamics showed that as we move away from the This review will provide the basis for a subsequent
settlement, the realization of ecosystem services by remote sensing study consisting of four steps: Initially,
the people also decreases. It is likely that extraction in the minimum size of declined forest patches that are
a planned manner with the help of stock and flow map detectable with current multi-spectral satellite data, will
will drastically reduce the unsustainable extraction be identified. Next, the influence of co-occurring land-
patter and help in sustainable measure of a natural oak cover classes on these results will be assessed by
forest. applying a multi-scale remote sensing approach
(including very high-resolution drone imagery and multi-
Key words: Flow and stock map, Ecosystem Services, Oak
forest, Biomass, Himalaya.
spectral satellite data). Then, the derived spatial patterns
of declined forest areas will be analyzed in relation to
SV/O-9 a range of explanatory variables. Ultimately, dendro-
chronological time-series of stress events will be
A Review of Forest Decline with a compared to spectral information available from historic
Landsat datasets to check the potential of reconstruc-
Special Focus on the Oak Forests in ting stress events over time using satellite data.
Iran Key words: Oak Decline Review, Multi-scale Remote Sensing,
Dendro-chronology, Persian Oak, Forest vitality.
Elham Shafeian1*, Fabian Ewald Fassnacht2 and
Hooman Latifi3
1
DAAD awardee and future PhD student at Karlsruhe
demanding species, to establish. Litter removal leads Key words: Oaks, Lithocarpus dealbatus, Quercus griffithi,
to poor acorn germination as exposed acorns get nitrogen resorption.
desiccated and become unviable. Understory
disturbance is also evidenced in soil nutrient levels, and SV/O-13
soil carbon was over 3% in undisturbed stands but under
1% in disturbed and degraded forests. Collection of
How Important are Oaks and Other
biomass from oak forests through an opening of canopy, Fagaceae Members in Undisturbed
and changes in regeneration patterns encourage the Sacred Subtropical Wethill
ingression of pine at the expense of oak. This is further
exacerbated by climate change which leads to Broadleaved Forests of Meghalaya,
increased drought period and thereby vulnerability to India?
fire. An increased frequency of fire further benefits
the fire tolerant pines thereby accelerating oak decline. S. Kharbhi1*, R.R. Gogoi1, B.R. Suchiang1, I.M.
Key words:Oak-decline, Central Himalaya, Lopping, Litter Nonghuloo1, K. Upadhaya1 and S.K. Barik2
removal. 1
Centre for Advanced Studies in Botany, North-Eastern Hill
University, Umshing, Shillong-793022, INDIA; 2CSIR-
SV/O-12 National Botanical Research Institute, Rana Pratap Marg,
Lucknow-226001, INDIA, Email: serrada2020@gmail.com
Nitrogen Resorption Efficiency of Two Most oak (belonging to genus Quercus) and other
Oak Species in a Montane Sub- Fagaceae (belonging to the genera Lithocarpus and
Tropical Forest of Meghalaya Castanapsis) species of north-eastern India are now
confined to subtropical wet hill broadleaved forests of
Namita Thapa1 * and Saroj Kanta Barik1,2 the region. The members of Fagaceae are best
1
represented in the undisturbed sacred forests of
Department of Botany, North-Eastern Hill University,
Meghalaya, India. These species provide a number of
Shillong-793022, Meghalaya, INDIA; 2CSIR-National
Botanical Research Institute, Lucknow-226001, INDIA,
ecosystem services such as checking soil erosion and
Email: namita_2424@yahoo.in, sarojkbarik@gmail.com nutrient leaching, providing natural habitat and feeds
to wild animals, and maintaining nutrient cycling in the
Leaf nutrient resorption before senescence is forest and adjacent agroecosystems. The seeds of many
considered an important mechanism for survival and Fagaceae members are one of the most important feeds
growth of plants in low nutrient environment. The for wildlife as well as human being. In addition, oaks
process is important as it directly influences the leaf with their large crown, bole and deep root system, play
litter quality, thereby affecting the overall forest an important role in regulating the water cycle and
productivity. In the present study, N- resorption in two carbon sequestration. Fagaceae contributes
oak species belonging to two different functional groups, significantly to the tree diversity of subtropical and
viz. Lithocarpus dealbatus, an evergreen tree species, temperate forests. The present article quantifies the
and Quercus griffithi, a deciduous tree species was relative contribution of various oak and other Fagaceae
studied in a montane subtropical forest of Meghalaya. species to total tree diversity in three undisturbed sacred
Labelled nitrogen (15N) was used to understand the forest stands of Meghalaya. In the study sacred forests
pattern of nitrogen uptake and resorption in the two viz, Nongbah, Ialong and Mawnai, a total of 10 oak
oak species. N- resorption efficiency of Q. griffithi and other Fagaceae species were recorded belonging
was 44.73±0.63 %, while that of L. dealbatus was to the genera of Castanopsis, Lithocarpus and
32.21 ±0.37 %. A higher foliar resorption efficiency of Quercus. In Nongbah sacred forest, Castanopsis
labelled nitrogen (15N) was also observed in Q. griffithi tribuloides contributed about 30% to the total tree
(65.61 ±1.55 %) than in L. dealbatus (54.53±1.21%). importance value (IVI 87.05), followed by Castanopsis
These results suggest that N- resorption was better in purperella (IVI 19.97). The dominant species in terms
the deciduous oak species than the evergreen species. of IVI value in Ialong sacred forest were Castanopsis
tribuloides (13.63), Quercus serrata (6.36) and determining the mean biomass value for each branch
Quercus griffithii (5.71). In Mawnai sacred forest, diameter class. The total branch biomass under each
Quercus griffithii (IVI 6.60), Castanopsis kurzii branch diameter class was determined by multiplying
(4.46) and Quercus semiserrata (3.72) were the mean branch biomass value for a particular branch
dominant. The tree population structure in each of these diameter class with the respective length of the
stand revealed that Fagaceae members constituted branches. The total branch biomass value was obtained
more than 90% in the higher DBH class trees. The by summing the biomass of the three branch diameter
contribution of Fagaceae members to total carbon stock classes. The mean leaf biomass was estimated for each
of the forest was very high due to their dominance in tree by taking leaf samples from three random branches
higher girth class trees. In view of their ecological and from each branch diameter class, and the total leaf
economic importance, oak forests should be prioritized biomass per tree was estimated by multiplying the total
for conservation. number of branches of the tree under each branch class.
Key words: Oaks, Importance Value, Sacred forests, Carbon The bole biomass was estimated using volume equations
stock. based on the DBH. The best fit model for volume (V)
estimation was of the form “V=a+bX, where X is DBH
SV/O-14 (R2=0.96). The mean bole volume per tree was 0.29
m3. The model can be applied to estimate biomass/
Estimating Carbon Stock in carbon and volume of the species in the diameter range
Lithocarpus dealbatus by Developing of 11-34.8 cm.
Species-Specific Allometric Models Key words: Lithocarpus dealbatus, Above ground biomass
(AGB), Carbon sequestration, DBH, north-eastern India.
Rashmi R. Gogoi 1 *, K. Upadhaya 2 and S.K. SV/O-15
Barik1,3
1
Department of Botany, North- Eastern Hill University, Conservation of Oaks: From the
Umshing, Shillong-793022, INDIA; 2Department of Basic
Sciences & Social Sciences, North-Eastern Hill University,
Perspective of a Wood Anatomist
Umshing, Shillong-793022, INDIA; 3CSIR-National Botanical
Research Institute, Lucknow-226001, Uttar Pradesh-226001, Prachi Gupta1 * and Sangeeta Gupta2
INDIA, Email: rasrekh.aj@gmail.com 1
Wood Anatomy Discipline, FRI, Dehradun, INDIA, Email:
prachi.gpta@gmail.com, sangeeta.fri@gmail.com
Oak forests have high potential for carbon
sequestration. For carbon stock estimation both at Natural disturbances caused by climate change,
species and ecosystem levels, it is desirable to have rising temperature and forest fire coupled with
species-specific allometric models, since each species indiscriminate anthropogenic activities such as lopping,
varies in its carbon sequestration potential. Lithocarpus free grazing of cattle, litter removal, fuelwood and
dealbatus being one of the dominant oak species in fodder collection have adversely affected oak forests
the montane subtropical forests of north-eastern India, in the Himalayas. Secondary xylem is indispensable
biomass model for the species is crucial for carbon for water conduction, storage and providing mechanical
pool assessment of these oak forests. The above ground support to the tree, yet the knowledge of its
biomass (AGB) and carbon values were estimated by microstructure is hardly taken into consideration while
harvesting 17 trees in the diameter at breast height formulating conservation strategies. In the present study,
(DBH) range of 11.0 - 34.8 cm. AGB of each tree the wood microstructure of Quercus leucotrichophora
was determined by summing the biomass values for and Q. acutissima was analysed in detail as per the
branches, leaves and bole. The biomass for branches terminology given by the International Association of
was determined separately for three branch diameter Wood Anatomists, taking samples from its naturally
classes i.e. < 5 cm, 5-10 cm and > 10 cm. Three occurring areas as well as those planted in FRI campus.
replicate samples from each diameter class having 15 Q. acutissima is a deciduous oak found in the eastern
cm length was oven dried till constant weight for Himalayas where snowline is at a higher altitude and
temperature and rainfall are high compared to the oak. The results of the analysis reveal that potential
western Himalayas which is dominated by evergreen oak cover in the absence of competition and disturbance
oak, Q. leucotrichophora. The study revealed that can be 63% more than its present distribution in the
these two species of oak occurring in contrasting study area. The regeneration pattern of oak was studied
environment at higher altitudes are anatomically through a structured field survey in five vegetation
different and both maintained their respective wood formations (grasslands, pine, pine–oak, open oak, and
microstructure even when planted at lower elevations dense oak), representing a theoretical successional
in Dehradun (668 m). Since wood anatomy is a highly sequence from early- to late-successional stages.
conservative feature and doesnot change in different Uniformly distributed sampling plots were used to
environmental conditions, the plants are thriving well gather information about the vegetation communities.
in Dehradun too. The present study suggests that these The proportion of oak regeneration was substantially
oak species have potential for ex-situ conservation at higher in sites which had not witnessed forest fires in
lower elevations and can be a part of botanical gardens the recent past. Our results show that the density of
and urban forestry as well. oak seedlings regenerating in early successional pine
Key words: Degradation, Wood microstructure, Lower forests was close to 900 seedlings per hectare, however
elevations, Ex-situ conservation. more than 75% of these regenerating seedlings were
grazed. Additionally, overexploitation of existing oaks
SV/P-1 trees via lopping affected the structural complexity of
the forest canopy, thereby increasing light availability
Regeneration and Potential on the ground and supporting the regeneration of light-
Distribution of Late Successional demanding early successional pine species, as opposed
to the desired oak. We conclude that with strategic
Quercus leucotrichophora (Banj Oak) management that restricts the current anthropogenic
in the Central Himalayan Forests of disturbances, the extent of oak forest in the study area
can be increased.
Uttarakhand
Key words: Himalaya, Succession, Oak, Regeneration,
1
Niyati Naudiyal * and Joachim Schmerbeck 2 Distribution.
1
School of Environment and Natural Resources, Doon SV/P-2
University, Mothorawala Road, Kedarpuram, Dehradun-
248001, INDIA; 2Chair of Silviculture, Institute of Forest
Sciences, Faculty of Environment and Natural Resources,
Altitudinal Distribution of Oak Species
University of Freiburg, Tennenbacher Str. 4, 79085 Freiburg, in Three Wildlife Sanctuaries in
GERMANY, Email: naudiyal.niyati@gmail.com, joachim.
schmerbeck@waldbau.uni-freiburg.de
Sikkim, India
We studied the potential distribution of Quercus Arun Chettri and Subhankar Gurung*
leucotrichophora (oak) and factors influencing its Department of Botany, Sikkim University, 6th mile, Tadong,
regeneration in mid-montane forests of Central Gangtok, INDIA, Email: achettri01@cus.ac.in
Himalaya. Oak forests (covering 38.5% of total 57516
ha of the study area) play an important role in The present study aimed to understand the
maintaining the local livelihoods and forest ecosystem altitudinal distribution of oak species in Maenam Wildlife
services (FES) in the region. The potential distribution Sanctuary (2100-3300 m) in South Sikkim, Fambonglho
of oak in the study area was identified using the Wildlife Sanctuary (1500 – 2200 m) in East Sikkim and
ecological niche modelling tool, MaxEnt. Presence Bersay Rhododendron Sanctuary (1900-3800 m) in
location of oak along with bioclimatic and physiographic West Sikkim. These sanctuaries are predominantly
spatial data derived from WorldClim and digital dominated by Oak species. Associated species such
elevation model (DEM), respectively, were used as as Daphniphyllum himalayense, Eurya japonica,
input variables for identifying the fundamental niche of Symplocos theifolia, Rhododendron arboreum, R.
griffithianum, Lyonia ovalifolia, Acer caudulatum of Castanopsis varied between 2.25 and 2.55 pg/2C.
grows along with oak species. At least 11 species of The genome sizes of these Castanopsis species are
oak have been reported from Sikkim by ENVIS, Sikkim. greater than that of Quercus species (range:- 1.88 to
The present study reports the distribution of nine oak 2.00 pg/2C). The genome size was used for
species in the study sites which fall under three genera. seggregating the three species. The three species had
The distribution of genus Castanopsis spp. ranged from significantly different genome sizes. This indicates the
1500 – 2400 m, Lithocarpus spp. from 1500- 2300 m successful application of flow cytometry results in
and Quercus spp. between 1500 – 2600 m at all the seggregating the elements within a taxa.
three sites. The oak species are: C. hystrix, C. Key words: 2C DNA content, flow cytometry (FCM),
lanceofolia, C. tribuloides, C. indica, L. Castanopsis, Fagaceae, Genome size.
pachyphyllus, L. elegans, L. fenestratus, Q.
lamellosa and Q. thomsoniana. SV/P-4
Key words: Wildlife sanctuary, Oak species, altitudinal
distribution, Sikkim Patterns of Diversity and Structure of
Oak Forests in Meghalaya along a
SV/P-3
Disturbance Gradient
Genome Size Estimation of
Aabid Hussain Mir1* and Krishna Upadhaya2
Castanopsis Species (Fagaceae) from 1
Department of Environmental Studies, North-Eastern Hill
Meghalaya, India using Flow University, Shillong-793022, Meghalaya, INDIA;
Cytometry 2
Department of Basic Sciences and Social Sciences, North-
Eastern Hill University, Shillong-793022, Meghalaya, INDIA,
Kiran*, Babita Joshi, Gopal Ji Tiwari, Satya Email: aabidm4@gmail.com, upkri@yahoo.com
Narayan Jena and Saroj K. Barik Oak dominated forests represent the subtropical
Plant Molecular Genetics Laboratory, CSIR-National broadleaved vegetation of Meghalaya and are closely
Botanical Research Institute, Rana Pratap Marg, Lucknow- linked with the livelihood of rural people. They are used
226001, INDIA, Email: kiranbhasker691992@gmail.com for timber, fuelwood and for edible nuts. Oak species
also play a major role in the maintenance of ecosystem
Castanopsis, commonly known as chinkapin is
stability, soil fertility, watershed, and local biodiversity.
one of the dominant genera of Fagaceae. In addition
The current study highlights the diversity and structural
to the timber value, the raw acorns of most species of
patterns of Oak forests of Meghalaya. Fifteen forests,
the genus are eaten by human and wildlife indicating
along a disturbance gradient (low, medium and high)
ecological and economic importance of the genus.
were sampled during the study. A total of 258 woody
Genome size determination is a pre-requsite for
(e”5 cm dbh), 217 shrubs and 210 herbaceous species
calculation of covergae of any de novo sequencing.
were recorded. Tree species richness of the forests
Many studies have shown that genome size is an
varied from 34-75, density ranged from 560-1164
important indicator for distinguishing different taxa.
individuals ha-1, and stand basal area from 7.4-77.4
There are various techniques for assessing genome
m2ha-1 . Shrub and herb species density varied from
size i.e. 2C DNA content. Among all these, flow
2912-1008 and 109200-378000 individuals ha -1
cytometry (FCM) is convenient, rapid and precise for
respectively. A total of 11 species of Fagaceae viz.
detecting small variation in genome size. In the present
Quercus kurzii Hance, Q. armata Roxb., Q. indica
study, the leaf samples of three species of Castanopsis
(Roxb. ex Lindl.) Drake, Q. brunnea H. Lév., Q.
viz., C. purpuella, C. tribuloides and C. armata were
tribuloides Sm., Q. dealbata Hk. f. & Th. ex Miq.,
collected from Jarain, Sohrarim and Umsining of
Q. elegans Bl., Q. glauca Thunb., Q. griffithii Hk. f.
Meghalaya, respectively, and were analyzed for their
& Th. ex Miq., Q. kamroopii D. Don and Q.
2C DNA content with the help of flow cytometer. The
semiserrata Roxb were recorded. These species
results revealed that the genome size of three species
contributed 28% and 48% to the overall density and
stand basal area of the forests, respectively. that have been high-graded limit choices between
Disturbance favoured the growth of Fagaceae clearfell and regenerate naturally or underplant with
members as evident by a positive correlation with Quercus seedlings to alter composition. In this
density except for Q. glauca, which showed a reverse presentation, we will review current restoration methods
trend. Density-diameter distribution of the species including innovations from improvements in planting
showed the reverse J shaped curve, while basal area stock and new silvicultural systems that restore
showed an irregular pattern. Forests exposed to low ecological function more quickly than traditional
disturbances had a high density and basal area in large afforestation with single-species stands. We also
diameter (>55 cm) class. Based on the density of consider the challenge of managing the understocked
seedling, saplings and adult individuals, the regeneration restoration stands that have resulted from current
of the oak species may be rated as good in disturbed federal policy and incentive programs. The yet
habitats, except Q. glauca, which prefer to grow in unaddressed challenges to sustainable management that
undisturbed forests. Most of the Fagaceae members would come from altered climate and sea level rise
were heliophytic in nature, hence can be used in have also been discussed.
ecological restoration programmes. Key words: Nuttall oak, cherrybark oak, bottomland
Key words: Community forests, Fagaceae, Subtropical hardwoods, functional restoration, Wetlands Reserve
broadleaf forests, Traditional forest management Program, climate change
SV/P-5 SV/P-6
accessible fuelwood and fodder biomass, only 11.9 % changing social dynamics this unregulated flow would
was extracted annually from the reserved forests. lead to unsustainable harvesting.
Though the current level of extraction from the reserved Key words: Beneficiaries, Oak forest, Flow of provisioning
forest seems to be sustainable in short term, with the services, Himalaya.
Session VI
Biomonitoring and Bioremediation
1
SVI/L-1 Deen Dayal Upadyay Gorakhpur University, Gorakhpur,
INDIA; 2CSIR-National Botanical Research Institute,
Economic Valuation of Ecosystem Lucknow, INDIA; 3UFZ-Helmholtz Centre for Environmental
Research, Leipzig, GERMANY; 4Universität Konstanz,
Ecological Services GERMANY; 5Deutsches Elektronen-Synchrotron (DESY),
Hamburg, GERMANY, Email: seema_mishra2003
Mir Mehrdad Mirsanjari @yahoo.co.in
Department of Environmental Sciences, Faculty of Natural Toxic metals and metalloids not only restrict plant
Resources and Environment, Malayer University, Malayer, growth and productivity, but also cause food chain
IRAN, Email: mmmirsanjari@malayeru.ac.ir contamination resulting in risk to human health. One of
Each ecosystem is a dynamic set of variables, the most relevant examples is the contamination of
each of which plays an essential role in the formation ground water with arsenic (As) in the Indian
of an ecosystem. In order to determine the economic subcontinent. It affects health of millions through
value of each ecosystem, it is necessary to pay attention drinking water and food chain contamination and
to all variables and their role, otherwise the value stated threatens the sustainability of food production.
does not indicate the true value of the ecosystem and Understanding the mechanism of As toxicity is crucial
in the long run, due to miscalculations and less economic for finding a sustainable solution to the problem, and
value, the space to destroy the creation It turns out.The determining the in planta distribution and speciation of
United Nations, the IMF, the World Bank, and the As are important steps in this process. Speciation and
Organization for Economic Co-operation and compartmentation of arsenic (As) in Ceratophyllum
Development (ICECD) have developed a “integrated demersum, a good laboratory model for shoot, and rice,
accounting system for the environment and economy”, the most As affected crop, was performed to explore
based on the results of previous studies on the mechanism of As toxicity. Speciation was analyzed
environmental asset valuation. In the recipe, chromatographically (high performance liquid
environmental assets consist of ecosystem goods and chromatography-[inductively coupled plasma-mass
services.Valuation, the ecosystem of two aspects, one spectrometry]-[electrospray ionization-mass
of the values of use and the other non-usable values spectrometry]) in whole-plant extracts and by tissue-
will be examined. Usage values are divided into two resolution confocal µ-X-ray absorption near-edge
groups: consumable (such as wood products) and non- spectroscopy (ì-XANES) in intact shock-frozen
consumable. Non-consumable values include direct hydrated root and leaves. The sub-cellular element
values (such as outing) and indirect values (such as distribution (compartmentation) was analyzed through
the value of water, soil and gas conservation). The µ-X-ray fluorescence (ì-XRF).Speciation through
ecosystem’s non-use values can also be seen as the chromatography revealed that in C. demersum, up to
conservation value of forests and the value of wildlife 60% accumulated As was in the form of thiol
conservation.In this research, we intend to determine complexed-AsIII. Most of which was in epidermis of
the economic value of the ecosystem based on the mature leaves as revealed by tissue resolved ì-XANES
ecological services function. of intact hydrated leaves. At sublethal concentration,
Key words: Ecosystem, Economic value.
As predominantly accumulated in the nucleus of the
epidermal cells, as revealed by ì-XRF analysis,
SVI/O-1 indicating replacement of P by As in DNA molecules,
providing first in vivo evidence for arsenic induced
Speciation and Compartmentation of DNA damage. While at lethal concentration, vacuole
was the main storage site of As, yet a significant
Arsenic in Plants and Mechanism of increase of unbound AsIII in mesophylls of young
its Sub-Lethal Toxicity mature leaves occurred. This small amount of As
reaching chloroplasts caused a strong and specific
Seema Mishra1,2,3,4 , Sanjay Dwivedi 1 , Rudra D. inhibition of tetrapyrrole biosynthesis and severe growth
Tripathi1, Gerald Falkenberg5, Jürgen Mattusch3 retardation. In rice plant, exposed to inorganic arsenate
and Hendrik Küpper4
(AsV) and methylated As, most of the AsV and was proven to be one of the important heavy metals
methylarsonate (MAV) were efficiently reduced to emissions sources in the present day urban scenario.
arsenite (AsIII) and MAIII respectively, but Using biological markers can potentially improve the
dimethylarsinate (DMAV) did not transformed. A large way in which exposure to environmental factors is
proportion of AsIII and MAIII were complexed with assessed. At present, however, only a few valid
thiols showing up to 20 and 16 As species respectively biological markers are available that can be effectively
in the roots. Many of them were identified as new As- used in epidemiological studies. The use of human hair
thiol species. Despite high complexation in root, more analysis technique can be used as a tool for monitoring
MA was translocated to shoot, with shoot/root transfer exposures to environmental pollutants. Of late, this
factor being in order DMA>MA>inorganic As in rice. technique has proved to be a well-suited biological
Further, the distribution study of As in frozen hydrated marker of occupational and environmental exposure
roots (both the root tip and the root hair zone) of rice of man. During the past three decades the
revealed that in the main root, As was mostly confined determination of trace elements levels in hair has been
to the endodermis. Whereas, in the young root branches the subject of continual interest in the biomedical and
apart from endodermis arsenic was also spread into environmental sciences (Arnold 1994 and Ciszewski
the cortex, particularly in younger, not fully 1997). In the present study concentration of four toxic
differentiated tissue including root tip. This shows that metals Pb, Cu, Cr and Zn was determined, using
As is taken up inadvertently by young tissues without standard methods, in human scalp hair residing near
proper control. In conclusion at a concentration that heavy traffic areas of Ajmer city (Rajasthan). Sample
can be easily attained in shoots of crop plants, As is were collected, decontaminated and digested before
already toxic to plants, i.e.it can inhibit pigment analysis using Atomic Absorption Spectrophotometer.
biosynthesis and cause DNA damage leading to The aim of the present study was to determine the
hampered growth and reported yield loss. This concentration of the chosen metals in hair of subjects
knowledge can be a starting point for targeted breeding residing near the sites of study and assess the
of more resistant plants. comparative burden of vehicular pollution in the selected
Key words: Arsenic, Ceratophyllum demersum, areas.
Compartmentation of arsenic, Rice, Speciation of arsenic, ì- Key words: Biomarkers, Vehicular pollution, Heavy metals,
XANES, ì-XRF. Scalp hairs.
SVI/O-2 SVI/O-3
was within the safe levels of Cd in edible tissues. The (phytolith) within and around the cells by grasses has
results pointed towards the efficacy of Cd emerged as a feature of tremendous potential in
phytoextration by P. stratiotes and suitability of this environmental monitoring and mitigation. As phytoliths
plant for the large scale remediation purposes. show variations in size in relation to changes in the
Key words: Cadmium, Phytoremediation, Antioxidants, ambient growth temperatures, they have proved to be
Uptake, Catfish bioassay. ideal markers for monitoring global warming and climate
change. As phytoliths occlude organic carbon of the
SVI/O-5 cytoplasm and stay unchanged in the soil for millenia,
they have tremendous potential in carbon sequestration.
Role of Grasses in Monitoring Global Also, persistence of biogenic silica in the soil converts
Warming and its Mitigation through the simple geochemical weathering of silica into a
biogeochemical cycle within which operates the shorter
Carbon Sequestration biological silica cycle. Owing to excellent preservation
down the millenia and diagnostic variations in
Amarjit Singh Soodan morphotypes, phytoliths have found an ever-increasing
Department of Botanical and Environmental Sciences, Guru role in understanding palaeoecology and
Nanak Dev University, Amritsar-143005, Punjab, INDIA, palaeoclimatology of the planet besides playing a role
Email: assoodan@gmail.com in contemporary environmental mitigation. The
The uncontrolled burning of fossil fuels has lead dynamics of phytolith accumulation in grasses with
to tremendous increase in green house gases (GHS’s), special emphasis on their role in carbon sequestration
mainly carbon dioxide and the resultant global warming shall be discussed in the lecture.
and its harmful effects. Scientists are looking for model Key words: Carbon sequestration, Environmental monitors,
organisms that could help monitor the pace, quantum Grasses, Palaeoecology, Phenology, Phytoliths.
and direction of climate changes taking place at regional,
national and global levels as also to reduce the SVI/O-6
concentration of GHG’s in the atmosphere and mitigate
their harmful effects. Grasses meet all the requirements Rhizo-Remediation of Hazardous
of environmental monitors including a cosmopolitan Waste: A Sustainable Approach for
distribution, high species diversity, habitat specificity
Environment Clean-up
and climatic zonation from tropical through subtropical,
temperate, alpine and the polar regions. Grasses display Bhawana Pathak
a closely coordinated cycle of well defined phenophases
in the vegetative and reproductive phenology that could School of Environment and Sustainable Development,
be utilized for assessing the impact of climatic factors Central University of Gujarat, Gandhinagar-382030, INDIA,
Email: bhawana.pathak@cug.ac.in
such as temperature, precipitation and duration of
sunshine. Grasses show special leaf anatomy correlated Industrial and technological revolution is an
with carbon assimilation cycles in cold and dry (C3) economic indicator for consideration as the principle
and hot and humid (C4) conditions. India is home to criteria for measuring progress. Technological
several cultivated (cereals and millets) and wild grasses revolution has lead to changes in the industrial product
that dominate the cultivated fields and vast ecosystems and processes. The development of product and
(grasslands) of the country. One only needs to earmark processes in the industries has generated wastes;
permanent environmental monitoring plots in natural enhanced environmental pollution and cause threat to
grasslands in different climate zones and select grass human life. The present treatment technologies -
species for experimental observations on phenological laboratory scale are insufficient to treat pollutants to
changes. Some such grass species have been identified acceptable level (contaminated land). Treatment
during our investigations in the North-West Himalayan technologies also resulted in the release of large number
region. Apart from possessing a well defined phenology, of natural and synthetic hazardous compounds (organic
accumulation of silica in the form of silica bodies and inorganic chemicals) due to lack of effective waste
treatment especially in industries like chemical, dyes, The arsenic contamination in soil and water has
textile, printing, construction and metal manufacturing increased up to levels that are unsafe for humans as
sectors. Considering all these flaws of physicochemical well as plants and animals. The problem of arsenic
treatment processes for remediation of hazardous contamination in ground water is severe in Bangladesh
compounds present in the industrial effluent, and in the state West Bengal of India. The release of
biotechnological approaches i.e bioremediation and industrial wastes, sewage waste and solid waste
phytoremediation are the emerging areas to remediate dumping has alleviated the problem of water
the environmental contaminants and are cost effective, contamination. Phytoremediation has been the cheap
eco-friendly and produce less toxic compounds and an and environmental friendly method to reduce
alternative method offers the possibility to destroy contamination, and thus, a hydroponic experiment with
harmful contaminants with the help of natural biological Phragmittes australis (Ph) and Vetiveria zizanioides
activity. Rhizoremediation is the superior biodegradation (Vt) was designed. The study was carried out using
of organic pollutants/ hazardous waste by root- different densities of accumulator (12 and 24 Ph, and
associated bacteria and fungi under the influence of Vt) in trays with arsenic concentration of 1 ppm, and
plant species. The use of plants and native remediation was observed with time duration (20 days).
microorganisms to degrade or remove pollutants has Sampling of water was done after every 2 days. It
established as an effective technology for in situ was found that arsenic concentration in water
remediation. The potential role of plants and associated decreased with time duration and density of the
rhizomicrobial population for in situ bioremediation of accumulators. Complete remediation of arsenic was
hazardous organic compounds is significant. The achieved after 20 days with 24 Ph and 24 Vt, and the
present paper focuses on the rhizoremediation of dye arsenic remaining in the medium was 0.04 and 0.25
compound and pesticides as case studies. The different ppb respectively. With these observations a 4 stage
factors influencing the plant-microbe-toxicant setup was made with 24 Ph and 24 Vt with same
interaction in soils is also highlighted. Rhizoremediation concentration of arsenic and sampling of water after
is a promising technology for the removal of different every 5 days. The arsenic concentration in water after
hazardous compounds from soil, and the developed 20 days was reduced, and was within the safe limit set
technology in the laboratory is helpful for transfer of by WHO with both accumulators. The 4 stage setup
technology from lab to land - a green technology for can be applied to arsenic contaminated irrigation water,
sustainable environment. reducing the further contamination of soil and toxic
Key words: Environmental pollution, Hazardous waste, effects on plants.
Treatment technology, Rhizoremediation, Technology Key words: Arsenic, Hydroponic, Phragmittes australis,
transfer - lab to land. Vetiveria zizanioides, 4 stage setup.
SVI/O-7 SVI/O-8
but can cause morphological, physiological and Telangana state was formed on 2nd June, 2014 and
biochemical dysfunctions in plants, animals and humans. project opening date was on 4th August 2016. It is
Phytoextraction is one of the possible method for situated in Yellampalli village, Ramagundam, Mancherial
mitigation of heavy metals from the environment. District (old Adilabad District) in Telangana State, India.
Helianthus annuus has been identified as a good metal The project is fourth largest one on Godavari river in
hyperaccumulating plant. Therefore, the present study Telangana. The project would supply water for 6tmc
has been carried out for screening of lead to NTPC Ramagundam city and Hyderabad City. After
hyperaccumulation potential of five varieties of H. the flood gates are installed, the project would store
annuus (DRSF-108, DRSF-113, Phule Bhaskar, about 20tmc of water. Barrage type of Dam height
LSFH-171 and KBSH-44). Seedlings of sunflower 263m, length 1180.7m, spillways 62. Construction cost
were treated with different concentrations (0, 50, 100, 5400 crore rupees. Its geographical coordinates
300, 500, 700 and 1000 ppm) of Pb(NO3)2. Plantlets 18050’45" North and 79022’05" East.
were harvested at 20, 40 and 60 days of time intervals.
The water samples were collected at 9 stations
Physiological studies such as root length and shoot
for analysis of physico-chemical and biological
length, fresh and dry weight of shoot and root of plants
parameters in three different seasons (rainy, winter,
performed. Concentration of Pb in various plant parts
summer) during 2017-18. Physical parameters like
were estimated by atomic absorption
appearance, color, odor, temperature, electrical
spectrophotometer. This study showed maximum
conductivity, turbidity, etc.; chemically characteristics
accumulation (693.69 ppm) of Pb in Phule Bhaskar
such as dissolved oxygen, biochemical oxygen demand,
and minimum accumulation (333.16 ppm) in KBSH-
chemical oxygen demand, total alkalinity, total hardness,
44 at 1000 ppm after 60 days. Phule Bhaskar
calcium, magnesium, chloride, free carbon-dioxide,
accumulated maximum (394.32 ppm) Pb in the roots.
phosphates were analyzed. Biological parameters like
Level of proline and polyphenol increased with
algae, fungal, bacterial, actinomycetes population were
increasing Pb concentration was reported in treated
analyzed and compared with IBS (10800) 2012. The
leaves of all varieties. The maximum proline and
total values indicate that river water belongs to the
polyphenol contents were 31.16 µmol g-1 and 7.15 mg
category of excellence to good water quality as per
g-1 respectively at 60 days. Thus, the results from this
IBS (10800) 2012.
study showed that among the five varieties, Phule
Bhaskar had a greater survivability and ability for lead Key words: Physical, Chemical, Biological parameter, BOD,
accumulation than rest of other varieties proving as COD.
good hyperaccumulating variety.
SVI/O-10
Key words: Helianthus annuus, Lead, Phytoextraction,
Heavy metals. Characterization of Iron Infused
SVI/O-9 Activated Carbon Developed from
Citrus limetta Biomass: An Application
Physico-Chemical and Biological for Theremoval of Fluoride from
Characteristics of Godavari River Aqueous Solution
Water at Sripada Yellampalli Project,
Mancherial Dist., Telangana, India Lata Verma*, Mohd. Ibrahim and Jiwan Singh
Department of Environmental Science, Babasaheb Bhimrao
B. Lalitha Kumari Ambedkar University, Lucknow-226025, INDIA, Email:
Department of Botany, Kakatiya University, Warangal, lataverma2501@gmail.com, mohdibra1144@gmail.com,
Telangana, INDIA, Email: lalitha21prasad@gmail.com jiwansingh95@gmail.com
Sripada Yellampalli Project foundation was laid In this study, a low cost adsorbent was prepared
on 28th July, 2004. It started in 2005, Andhra Pradesh. at 500oC temperatures from pulp of Citrus limetta and
applied for the treatment of fluoride contaminated around Kanpur from two points (middle of the river
water. Prepared bioadsorbent was characterized stream and a discharge point) at each location. Metals:
through X-ray diffraction, Fourier transform infrared Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Mo, Cd, Se and Pb
spectroscopy, pHzpc and scanning electron microscopy. were detected in all the samples. The metals showed
This bioadsorbent shows very effective removal of no noteworthy disparity with respect to sampling
fluoride as observed in batch adsorption study which locations as well as discharge points. However, the
was performed at different temperature (298K, 308K, concentration of the metals varied with season, being
318K and 328K), pH (4-10), dose (0.5, 1.0, 2.0, and higher in summer and lower in rainy season. Out of all
3.0 g L-1) and concentrations (1-30 mg L-1). The result the metals found in the study site, Cr, Fe Cu, As and
shows maximum removal of 86% with ACP-500. Pb concentration in all the sample during each season
Adsorption isotherm, intraparticle diffusion, at all the study sites are higher than the prescribed
thermodynamics and kinetics reaction were also limit by Bureau of Indian Standard (BIS) and World
studied. For kinetic study, pseudo-second-order kinetics Health Organization (WHO) for drinking, aquatic life
was best fitted and in adsorption study it was observed and irrigation. Result of pollution indices shows that
that Langmuir isotherm model well fitted. Adsorption river water is not suitable for drinking and aquatic life
process was found to be exothermic, it represents but suitable for irrigation purpose only.
removal percentage decreased with increasing Key words: Metals, Seasonal variation, River Ganga,
temperature. Regeneration study revealed that the Pollution indices.
bioadsorbent can be reused three to four times. The
authors are grateful to the SERB-DST, Government SVI/O-12
of India (Project No. ECR/2016/001924) for providing
financial support for this work. Role of Bacteria in Remediation of
Key words: Adsorption, Isotherm, Kinetics, Fluoride, Indoor Pollution with the Help of
Characterization.
Aglaonema commutatum Schott
SVI/O-11
Seemaa Ghate
Monitoring and Assessment of Know How Foundation, C-602, Vaidehi Enclave, Bavdhan,
Pollution Load in Terms of Heavy Pune, Maharashtra, INDIA, Email: drseemaaghate@
gmail.com
Metals in Surface Water of River
Role of Sphingomonas, gram negative bacteria
Ganga around Kanpur: A Study for was examined in remediation of Indoor pollution with
Suitability of this Water for Different help of Aglaonema commutatum. Sphingomonas was
isolated and inoculated in the medium in which
Utilities experimental plants were grown. Aglaonema was
tested against pollutants released from household
Vandana Singh,* Nem Kumar Nagpoore, Jai
products like mosquito coil and incense sticks. It was
Chand and Alok Lehri
found that Sphinomonas helped plant in the activity of
Central Instrumentation Facility, National Botanical Research phytoremediation. Exposure to Incense sticks showed
Institute, Rana Pratap Marg, Lucknow-226001, INDIA, Email: no absorption of any pollutant under consideration.
singh_vini02@yahoo.in Exposure to smoke from mosquito coil showed that
A study was conducted during April, 2016–March, levels of allethrins absorbed by plant, were very high,
2017 to evaluate the surface water quality of river up to 20-30 ppb as tested on GCMS. When GCMS
Ganga around Kanpur. The samples were analyzed analysis of same plant was done after 20 days it was
for a number of physico-chemical parameters using observed that levels of allethrin reduced to 0. Allethrins
standard laboratory procedures and giving prime plunge may be digested by Aglaonema plant with help of
to determine the heavy metal concentrations of surface Sphingomonas, as this bacteria helps phytoremediation
water at five different locations of the river Ganga activity. Further research on this line is in the channel.
Key words: Sphingomonas, Aglaonema, Indoor pollution, strongly suggest that greater phytoextraction can be
Mosquito coil, Incense sticks, Phytoremediation. accomplished by the application of environment friendly
and biodegradable chelant NTA.
SVI/O-13
Key words: NTA, Chelant, Phytoextraction,
Phytoextraction of Lead from Soil Hyperaccumulator.
application of silicon (soil/foliar), enhanced the growth results, physicochemical treatment combined with
and yield of rice plants by increasing the activity of biological treatment showed some sort of efficiency
antioxidants enzymes and simultaneously leading to rather than physicochemical treatment with
reduced accumulation of arsenic in grains. Parallel sophisticated techniques like electro coagulation with
studies revealed that foliar application of silicon is more chemical coagulation. However, it is very clear from
beneficial to the plants rather than soil based application. the study that industries have made significant efforts
The study concludes that the silicon application in the to comply with tolerance limits of industrial discharges
form OSA had a significant contribution in increasing to inland surface waters with high organic waste load.
the quality and quantity of the rice produce from the Further, it was evident that combined system of physical,
heavy metal affected areas. chemical and biological treatment facilities has shown
Key words: Rice, Arsenic, Silicon, Heavy metal stress. a remarkable efficiency in achieving the expected
targets.
SVI/O-15 Key words: Wastewater, Pollution, Industrial discharges,
Textile processing, Electro coagulation.
Assessment on Environmental Issues
Related to Water Pollution Associated SVI/O-16
with Textile Processing Industries Heavy Metal and Prospects of
Indehiwala-Mt. Lavinia and Moratuwa Phytoremediation in India
Municipal Council Areas in Sri Lanka
Jamshed Zaidi and Amit Pal*
1 2
M.R. Namalie and T.K. Weerasinghe Institute of Environment & Development Studies,
1
Central Environmental Authority, Denzil Kobbekaduwa Bundelkhand University, Jhansi-284128, INDIA, Email:
Mawatha, Battaramulla, SRI LANKA; 2Centre for apu13@rediffmail.com, jamshedphd@gmail.com
Environmental Studies and Sustainable Development, The Many fold increase of population and the pace of
Open University of Sri Lanka, Nawala, Nugegoda, SRI industrialization and urbanization have caused the
LANKA, Email:namalie44@gmail.com
widespread dissemination of heavy metals in our
The textile processing and garment industry is environment. Heavy metals are among the most
one of the most important industrial sectors in Sri Lanka. hazardous contaminants that may be present within the
This study mainly focused on assessment of aquatic ecosystem. They pose a serious threat to aquatic
environmental issues related to water pollution during biodiversity, and severe health risk for humans due to
textile processing while introducing appropriate the intake of contaminated water. Several existing
mitigatory measures. During the study, existing pollution technologies have been used to reduce aquatic
control measures to obtain Environmental Protection pollution, but they are generally not cost-effective, are
License from the Central Environmental Authority of labor-intensive and generate secondary waste. It is an
16 industries with different scales (large/medium and intriguing option which is an eco-friendly and affordable
small) were investigated. A detailed study was carried technique. Phytoremediation, which is growing to be
out in 07 industries including wastewater treatment the most attractive innovation in this area, utilizes plants
systems, volume of discharge or consumption of water, for the evacuation or alteration of ecological
work force, production capacity and mode of discharge contaminants, causing them to become innocuous. In
of treated waste water to the environment while this chapter, we summarize use of this technique, which
analyzing the treated effluent. The obtained results may play a strong role in the elimination of heavy metals
were statistically analyzed to find out suitable from our aquatic environment using some existing
relationships and possible significant differences. Seven macrophytes available in Bundelkhand region of Uttar
critical parameters such as temperature, colour, pH, Pradesh.
BOD, COD, TSS and oil and grease content were Key words: Heavy metal, Phytoremediation, Aquatic
measured for a period of six months. According to the macrophytes.
SVI/O-17 SVI/O-18
quality may degrade in the near future due to increasing the two villages. Apart from that, analysis of As in rice
anthropogenic inputs in the river basin may cause grains of five popular varieties, collected from residents
irreparable ecological approach. of two villages, presented occurrence of high As
Key words: Hydrological status of river, Alluvials/ sediment concentrations in rice grains viz- Gosai (0.95 mg kg-1),
transport, Potential heavy metal, Ecological approach. Satabdi (0.79 mg kg-1), Banskathi (0.60 mg kg-1), Kunti
(0.47 mg kg-1) and Ranjit (0.29 mg kg-1). Furthermore,
SVI/O-19 Gosai and Satabdi were found the most popular
varieties being consumed by local people. This survey
Arsenic Hazard Assessment in based assessment is a primary observation needed to
Groundwater-Soil-Rice System in Two be further immediate action for arsenic remediation.
Villages of Bengal Delta Basin, West Key words: Average daily dose, Arsenic, Groundwater,
Hazard quotient, Rice, Soil.
Bengal, India
SVI/O-20
Munish Kumar Upadhyay1* , Arnab Majumdar 2 ,
Anil Barla2, Sutapa Bose2 and Sudhakar Srivastava1 Relative Effectiveness of Different
1
Institute of Environment & Sustainable Development, Iron Complexes in Functional Iron
Banaras Hindu University, Varanasi-221005, Uttar Pradesh,
INDIA; 2Earth and Environmental Science Research
Availability in the Rapseed Plants
Laboratory, Department of Earth Sciences, Indian Institute
of Science Education and Research, Mohanpur-741246, West Rajesh K. Tewari
Bengal, INDIA, Emails: munish007up@gmail.com, Department of Botany, University of Lucknow, Lucknow-
arnabmajumdar891@gmail.com, barla.neal@gmail.com, 226007, INDIA, Email: rktewari_bot@yahoo.com
sutaparai@gmail.com, sudhakar.srivastava@gmail.com
Iron (10 µM) in complex with aromatic ligands
Arsenic (As) problem in groundwater is a global (10 µM) such as salicylic acid, 8-hydroxyquinoline, (+)-
menace since last three decade. Arsenic can also be catechin did not provide enough Fe to plants, which
build up in soil and crop plant through irrigation process. was indicated by decreases in the concentrations of
West Bengal is an important As pollution hotspot in chlorophyll and carotenoids, activities of catalase and
India. To understand the actual reality about As problem, ascorbate peroxidase in the leaves and activated ferric
present study conducted and demonstrated As chelate reductase activity in the roots. These plants
concentrations in soil, groundwater and rice grain exhibited cell death and accumulated ROS. Moreover,
samples of two remote villages, Sarapur and Chinili, they also enhanced DNase, protease and caspase-3-
Nadia district, West Bengal, India. The study was like activity. Iron (10 µM) in complex with juglone (10
conducted in Sarapur and Chinili villages of Dewali µM) was highly toxic as Brassica napus plants
Gram panchayat in Nadia district, West Bengal, India. collapsed within an hour of treatment. However, at 10
The study also comprised a survey among people µM Fe and 2 µM juglone supply plants did not show
residing in these two villages to correlate the knowledge any toxic effect. While B. napus plants treated with
among villagers about the problem with the observed aromatic organic ligands accumulated enough Fe to
results. The data of consumption of rice per day in the support normal metabolic function, they exhibited Fe
survey was used for the measurement of average daily deficiency-like effects. Iron (10 µM) in complex with
dose (ADD) and hazard quotient (HQ). The results non-aromatic organic ligands (10 µM) such as EDTA,
revealed the presence of As in high concentrations in citrate and deferoxamine supplied sufficient functional
groundwater [124.50 µg L-1 (Sarapur) and 138.20 µg Fe to the plants for their metabolic function and growth.
L-1 (Chinili)] and soil [47.7 to 49.3 mg Kg-1 (Sarapur) These plants did not produce any visible toxic effect.
and 57.5 to 62.5 mg Kg-1 (Chinili)]. So, people with
Key words: Iron complexes, EDTA, Citrate, 8-
skin related As toxicity symptoms were also stated in
hydroxyquinoline, (+)-catechin, Juglone, salicylic acid.
SVI/O-21 SVI/O-22
Key words: Algae, Waste water, Lipid, Constructed wetland, India, Andhra Pradesh and Karnataka are suffering
Phycoremediation. with ground water As contamination. Low level of As
(up to 17 µg l-1) has also been reported in Tamil Nadu
SVI/P-1 from South India. Many of the states like Jammu and
Kashmir, Uttarakhand, Odisha, Gujrat, Kerala,
Increasing Ground Water Arsenic Telengana, Goa etc. are still unexplored for ground
Contamination in India: A Future water As contamination. Thus, according to current
Danger reports out of 640 districts in India, 141 districts are As
affected (As >10 µg l-1), and 120 are above 50 µg l-1.
Seema Mishra1,2*, Sanjay Dwivedi2, Amit Kumar3, Owing to the toxicity of As to humans as well as its
Reshu Chauhan 2 , Surabhi Awasthi 2 , and R.D. impact on crop productivity As contamination in ground
Tripathi2 water present a great national challenge.
1
Universität Konstanz, Mathematisch-Naturwissens Key words: Arsenic, Ground water arsenic contamination,
chaftliche Sektion, Fachbereich Biologie, Postfach M665, Indian states.
D-78457 Konstanz, GERMANY; 2Plant Ecology and
Environmental Science Division, CSIR-National Botanical SVI/P-2
Research Institute, Lucknow-226001, INDIA; 3Department
of Botany, Lucknow University, Lucknow-226007, INDIA; Appraisal of Heavy Metals in
4
UFZ-Helmholtz Centre for Environmental Research, Roadside Soil from Surroundings of
Department of Analytical Chemistry, Permoserstr 15, D-04318
Leipzig, GERMANY, Email: seema_mishra 2003@yahoo.co.in Ropar Wetland, Punjab, India
India is consisting of 29 states and 7 union Sakshi1*, Avinash Kaur Nagpal 1 and Inderpreet
territories, including a national capital, Delhi. Elevated Kaur2
concentration (>10 µg l-1) of arsenic (As) in ground
1
water of many states of India has become a major Department of Botanical and Environmental Sciences, Guru
concern in recent years. Up to now about 0.2 million Nanak Dev University, Amritsar-143005, Punjab, INDIA;
2
Department of Chemistry, Guru Nanak Dev University,
ground water samples have been analyzed for As
Amritsar-143005, Punjab, INDIA, Email: svsharma69@
contamination from all over India by various researchers yahoo.com, avnagpal@rediffmail.com, inderpreet11@
and Government agencies. About 90% of these cover yahoo.co.in
only the Eastern part of India while several states and
union territories are still unexplored. However, from In the present study, an attempt was made to
the available data, ground water of eighteen Indian determine the concentration of various heavy metals
viz. cobalt, cadmium, lead, zinc, copper and chromium
states and three union territories has been found to be
in roadside soil samples from the areas surrounding
As contaminated to different extents through natural
Ropar wetland, Punjab, India. Soil samples were
or anthropogenic origin. Among these, As >300 µg l-1
collected from three locations along roadsides of the
has been reported from at least one locality representing
areas surrounding Ropar wetland in March,
fourteen states. The maximum level of As (7350 µg l- 2013.Contents of different heavy metals were assessed
1
) in ground water has been reported from a highly using flame atomic absorption spectrometer. It was
industrialized area, Patancheru in Medak district of observed that contents of different heavy metals in
Andhra Pradesh. However, the gravity of problem is roadside soil samples ranged as 1.14 to 14.20 mg kg-1
more in West Bengal followed by Bihar and Uttar for Co, 0.26 to 1.10 mg kg-1 for Cd, 5.10 to 14.13 mg
Pradesh. Five out of eight North-Eastern states are kg-1 for Pb, 20.76 to 45.07 mg kg-1 for Zn, 9.16 to 15.16
also affected by As contamination. Manipur is ranked mg kg-1 for Cu and 1.30 to 16.35 mg kg-1 for Cr. Order
first and Assam as second followed by Arunachal of different metals based on their metal contents was:
Pradesh, Tripura and Nagaland. The ground water in Zn (31.36 mg kg-1) > Cu (11.82 mg kg-1) > Co (9.54
these regions is naturally As enriched, and therefore mg kg-1) > Pb (8.38 mg kg-1) > Cr (7.04 mg kg-1) > Cd
wide spatial distribution of As has been found in these (0.68 mg kg-1). Further, it was found that 33% samples
areas. In North India, Punjab and Haryana and in South
showed Pb content above typical soil concentration of diatom studies not only help in a realistic assessment
10 mg kg -1 , whereas 67% samples had higher of pollution but it also assists in carbon sink potential.
concentrations of Co than the typical concentration of Key words: Algae, Diatom, CO2, Pollution, Futala lake.
8 mg kg-1. All samples showed Cd content to be much
higher than the typical soil concentration of 0.06 mg SVI/P-4
kg-1. The high concentrations of these metals can be
attributed to vehicular emissions. Effect of Arbuscular Mycorrhizal
Key words: Heavy metals, Flame atomic absorption Fungi on Growth and Cadmium (Cd)
spectrometer, Roadside soil samples.
Uptake by Solanum nigrum Grown in
SVI/P-3 Cd Contaminated Soil
Diatoms as an Important Indicators of P. Sharma1, H.P. Singh1* and D.R. Batish2
Pollution, Futala Lakestudy, Nagpur, 1
Department of Environment Studies, Panjab University,
India Chandigarh, INDIA; 2Department of Botany, Panjab
University, Chandigarh, INDIA, Email: hpsingh_01
M.P. Meshram*, K.J. Cherian and S.D. Dhyani @yahoo.com
CSIR-National Environmental Engineering Research It is well known that plant–microbe interactions
Institute, Nagpur-440020, INDIA, Email: m.manisha1029 in the rhizosphere play an important role in soil fertility
@gmail.com and plant health. Arbuscular mycorrhizal fungi (AMF)
The rising carbon dioxide (CO2) emission leading are the most important microbial symbioses for most
to global climate change is one of the greatest plants roots that facilitate nutrient uptake, water
environmental challenges that the world faces today. relations and above-ground productivity in exchange
Most phytoplankton mainly diatom algae is one of the for organic carbon from their host plants. They can
marine and freshwater plants having higher enhance plant growth and resistance to toxicity
photosynthetic efficiencies than any terrestrial plants produced by heavy metals, affect their bioavailability
and is able to capture carbon more efficiently. Algae in soil and uptake by plants and thus have emerged as
utilize bicarbonate directly or indirectly, by its the most prominent symbiotic fungus for contribution
disassociation into carbon dioxide and in so doing cause to phytoremediation. A pot experiment was conducted
an increase in pH of the water and precipitation of in a dome to determine the effect of AMF association
calcium carbonate. Diatoms have the ability of to do with the roots of Solanum nigrum in soils with different
this, that within a dense surface bloom an intense
concentrations of cadmium (0, 10, 25, 45 mg Cd kg-1
carbondioxide demand occurs. In western area of
soil). Control and two experimental sets, one having
Nagpur, ancient Futala Lake exists since 200 years,
different concentrations of cadmium and the other
spread over 60 acres and was selected as the study
having different concentrations of cadmium and AMF
area. In this context an attempt was made to study the
diatom diversity of Futala lake. The surface water (from plant roots) were set up. Growth, biomass,
samples were collected and monitoring was carried photosynthetic efficiency, Cd uptake and accumulation
out seasonally during Jan. 2014 to Dec. 2014. Collected by the plants were measured after six weeks. A better
materials were mounted with Iodine solution and accumulation and tolerance of Cd in the AMF-treated
observations were recorded under the microscope and plants was seen as compared to the control plants and
identified the taxa as per standard literature and the experimental plants with Cd only. The results
encouraging results have been obtained. The 21 genus indicated that S. nigrum associated with AMF
of Diatoms were recorded. Thus the Diatom population effectively enhanced the Cd uptake by plants and offer
is represented with 41 species belonging to 21 genus a novel strategy in microbe-assisted phytoremediation
of which 5 forms were of pollution indicators as per for Cd-contaminated soils.
palmer index. However, the species belonging to Key words: AMF, Hyperaccumulator, Heavy metals,
Bacillariophyceae were more, it is clear that the Rhizosphere, Toxicity.
diatom density was higher in the Futala Lake. Therefore
SVI/P-5 SVI/P-6
sediment can be applied for monitoring of heavy metal decreased biosotption of Pb and Cd, while other anions
pollution in sediment, and the magnetic properties of and cations did not show significant effect. Maximum
leaf and bark for monitoring of atmospheric deposition. desorption of Pb and Cd was achieved in the presence
Moreover, the magnetic properties of sediment and of EDTA and HNO3, respectively. Results also showed
mangrove organs (root, bark, leaf) can be applied for that the test biosorbent could be repeatedly used up to
the evaluation of ecological impacts of heavy metal on six biosorption/desorption cycles without significant loss
mangrove trees, and for conservation of this sensitive of its initial metal adsorption capacity. An analysis of
ecosystem in the shore regions. biosorption of Pb and Cd by using free living,
Key words: Heavy metal, Boushehr shore, Magnetic immobilized living and non-viable forms of N.
properties, Bioaccumulation, Mangrove trees. muscorum revealed that immobilized biomass of N.
muscorum was able to remove more metal as compare
SVI/P-7 to other forms.
Key words: Adsorption isotherms, FTIR spectra, Heavy
Biosorption of Lead and Cadmium by metals, Kinetics, Nostoc muscorum.
Cyanobacteria and Optimization of its
SVI/P-8
Biosorption Potential
Role of Algae in the Assessment of
Sonal Dixit1* and D.P. Singh2
1
Pollution Status of River Ganga at
Department of Botany, University of Lucknow, Lucknow-
226007, INDIA; 2Department of Environmental Science, Kanpur, India
Babasaheb Bhimrao Ambedkar University, Raibareilly Road,
Lucknow-226025, INDIA, Email: sonal.env@gmail.com, Vinod Rishi 1 , Bindeshwari Prasad 2 * and A.K.
dpsingh_lko@yahoo.com Awasthi3
1
The present study relates to the use of Faculty of Science and Environment, Mahatma Gandhi
cyanobacterium Nostoc muscorum as a model system Chitrakoot Gramodaya Vishwavidyalay, Satna, M.P., INDIA;
2
for removal of lead (Pb) and cadmium (Cd) from aquatic Department of Zoology, Sukdev Singh Luvkush PG College,
systems and influence of different factors on the Baberu, Banda, U.P., INDIA; 3Department of Botany,
Brahmanand College, Kanpur, U.P., INDIA, Email:
biosorption process. The maximum sorption of Cd
vinod.rishi25@gmail.com, bindeshwari1456@gmail.com,
(83.2%) and Pb (90.3%) was achieved at 60 and 80 ashok.awasthi15@gamil.com
ìgml-1 initial concentrations of respective metal, within
30 and 15 min, pH 5 and 6 respectively, with optimum Algae are the morphologically diverse group of
cyanobacterial biomass (2.8 g L -1 ) and at 40 o C autotrophic organisms. They are found in almost all
temperature. A significant proportion of Cd and Pb kinds of habitats and are especially flourished in the
removal was mediated by surface binding of metals organically polluted waters. Some algae highly tolerated
(79.2% Cd and 85% Pb), rather than by intracellular to the organic pollution and also act as indicator of such
accumulation (4% Cd and 5.3% Pb). N. muscorum kind of pollution. Algal samples were collected from
has maximum amounts of metal removal (qmax) capacity two sampling sites viz. Bithoor (Upstream) and Jajmau
of 833 and 666.7 mg g-1 protein for Pb and Cd, (Downstream) of river Ganga at Kanpur during the
respectively. The kinetic parameters of metal binding period from March 2016 to February 2017 and total 51
revealed that adsorption of Pb and Cd by N. muscorum genera spread over 228 species belonging to the class
followed pseudo-second-order kinetics, and the Chlorophyceae, Bacillariophyceae, Cyanophyceae and
adsorption behaviour was better explained by Langmuir Euglenophyceae have been identified. The most
isotherm model. The surface binding of both the metals dominating group was Cyanophyceae followed by
was apparently facilitated by the carboxylic, hydroxyl, Bacillariophyceae, Chlorophyceae and Eugleno-
and amino groups as evident from Fourier transform phyceae. During the present investigations 34 genera
infrared spectra. The presence of anions and cations and 36 species were identified as indicator of organic
showed that existence of Ca and EDTA resulted in pollution on the basis of Palmer’s Pollution Index (PPI)
for the assessment of pollution status of river. The PPI hyperaccumulators and enhanced Fe, P, K, and Zn
of both genus and species for Bithoor were 29, 28 and uptake. Different plant growth responses to As among
Jajmau 31, 32, respectively. Thus, the river Ganga at hyperaccumulators PV and PM and non-
Kanpur was highly polluted due to the organic pollution. hyperaccumulator PE may help to better understand
Key words: Algae, Organic pollution, PPI. why hyperaccumulators grow better under As-stress.
Key words: Hyperaccumulators, Pteris vittata, Arsenic,
SVI/P-9 Phytoremdeidation.
SVI/P-11 SVI/P-12
promoting microbial strains can be isolated for and amidase pathways. Cyanide dihydratases readily
developing a carrier based consortia formulation and convert cyanide to relatively non-toxic formate, does
creating a bio-inoculant arsenic remediation product not require any cofactor and its activity is enhanced by
for their soil applications/field trials. This approach will the presence of heavy metal (Cr+, Fe3+) ions. Native
serve dual purpose in reducing the arsenic uptake by novel microbial communities can be utilised to harness
the plants and promoting plant growth Such carrier these potential enzymes which degrade cyanide
based microbial formulation will nullify/reduce arsenic effectively and can further remediate cyanide from
toxicity and gain safe and sustainable agriculture. contaminated effluents. Additionally, the identified
Key words: Arsenic, Microbes, Consortia, Formulation, Plant micro-organisms can be used as a source of potential
growth promotion. genes for development of transgenic plants having
enhanced cyanide detoxification capability.
SVI/P-13 Bioremediation approach utilizing microbial consortium,
fast metabolizing and transgenic plants will help in
Bioremediation as a Prospective reducing cyanide concentration in effluents below the
Technology to Remove Cyanide from permissible level. As such it will result in development
of a viable and effective integrated bioremediation
Coke Oven Plant Effluent of Steel technology for the cyanide contaminated wastewater
Industry and other matrices.
Key words: Cyanide, Bioremediation, Fungi, Wastewater,
Saloni Rishi1 *, Pankaj Kumar Srivastava 1 , R.D. Steel.
Tripathi1, R.N. Kharwar2 and S.K. Verma2
1
CSIR-National Botanical Research Institute, Rana Pratap SVI/P-14
Marg, Lucknow-226001, INDIA; 2Centre of Advanced Study
in Botany, Institute of Science, Banaras Hindu University, Remediation of Dyes in Water using
Varanasi-221005, INDIA, Email: rishi.saloni@gmail.com; Biogenic Nanoparticles
drpankajk@gmail.com, tripathird@gmail.com, rnkharwar@
gmail.com, skvermabhu@gmail.com Akanksha Pandey* and Pankaj Kumar Srivastava
Cyanide is a poison and well-known as a Environmental Technologies, CSIR- National Botanical
metabolic inhibitor being released in large amounts (2- Research Institute, Rana Pratap Marg, Lucknow-226001,
20 mg l-1 ) in wastewaters of different industrial INDIA, Email: akanksha.p1995@gmail.com
processes related to metal plating, coke oven, and
With the rapid growth of population and increasing
jewellery industry for gold and silver extraction. Its legal
urbanization and industrialization, the environment
discharge limit as per MINAS is 0.2mgl-1. Since
pollution is becoming a serious concern worldwide. The
cyanide is a fast acting toxin, therefore cyanide-
inevitable and unjudicious release of various types of
containing effluents cannot be discharged without being
pollutants into water bodies from a wide range of
subjected to treatment for its content beyond the
industries and chemical factories has been the main
permissible limit. Chemical treatments are often cost
cause of environment pollution. In order to ensure the
intensive and reckoning additional hazardous chemicals
better quality of people’s living standard, how to ensure
like chlorine to the environment. Bioremediation could
the pollutant-free water resources is one of the most
be a felicitous alternative because of its speed,
difficult challenges in the 21st century. Among various
simplicity, cost effectiveness, and eco-friendly
contaminants, dyes are one of the most widely used
mechanisms. Certain bacteria and fungi metabolize
chemicals that are mainly discharged from industries
cyanides by producing different enzymes like cyanide
viz; textile, cosmetic, paper and leather, etc. Even at
hydratase, monoxygenase, nitrilase etc. Trichoderma
minute content (<1ppm), dyes are posing detrimental
spp. has been demonstrated to produce two important
threat to ecosystem and human health risks. The
cyanide-degrading enzymes, rhodanese and cyanide
wastewater containing dyes is one of the most difficult
hydratase. Fusarium solani under alkaline conditions
industrial wastewaters to treat. Recently,
(pH 9.2-10.7) degraded cyanide by cyanide hydratase
Nanotechnology has emerged as an efficient compounds as root exudates viz., L-arginine, DL-
technology for the remediation of different leucine, DL-methionine may change the chemical and
environmental pollutants from water. Green synthesis biochemical properties of soils and thus, regulating the
of nanoparticles can be done for rapid degrade atoms structure of soil microbial community exists in the
of dyes in wastewater. Various nanoparticles such as vicinity of plant rhizosphere. Root exudates of different
iron, palladium and cerium dioxide using Camellia plant species may promote growth of fungal strains as
sinensis, Boswellta serrata , and Azardirachta indica well. There was a requirement to increase numbers of
extracts have been reported successfully for the viable fungal cell counts in the pure fungal cultures for
remediation of anthroquinone, azo and various synthetic consortium preparation. Above mentioned amino acids
dyes like Rhodamine B, methylene blue etc. Therefore, were used to promote fungal cell counts and resulted
the idea of biogenic green synthesis of nanoparticles in increased fungal growth by one fold log units. Despite
for the remediation of different hazardous dyes from conventional methods, an alternative method based on
wastewater may help in achieving an eco-friendly the transformation and colorimetric quantification of
remediation technology for dyes. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet-
Key words: Nanotechnology, Dye pollution, Remediation, razolium bromide] was used to determine the impact
Green synthesis. of amino acids on the increase of viable fungal cell
counts. It was concluded that amino acids in the form
SVI/P-15 of root exudates may increase viable fungal growth
which was validated by the sensitive evaluation using
Role of Root Exudates to Promote MTT assay in their pure cultures.
Fungal Growth in an Arsenic Remedia- Key words: Arsenic, Fungi, Root exudates, MTT assay,
Amino acids.
ting Consortium and Validation of
Growth using MTT Assay SVI/P-16
correlation of different soil characteristics with fractions carcinogen. Various strategies have been adopted from
of bioavailable arsenic in contaminated soil. Soil samples time to time to reduce arsenic contamination viz; use
were collected from 10 different sites of West Bengal, of surface water for irrigation, use of physico-chemical
India. Three extractants, such as water, sodium treatment options etc. These days bioremediation
bicarbonate (NaHCO 3 ), di-hydrogen ammonium approaches to reduce the arsenic level at the
phosphate (NH4 H2 PO 4), were used to extract the contaminated sites are gaining popularity being eco-
different bioavailable fractions viz water soluble, friendly and cost effective. Mycoremediation of arsenic
weakly adsorbed, specifically adsorbed and residual contaminated sites has been found to be effective as
of arsenic, respectively. The results showed that the fungi are predominant living microbiota in soil and
relative abundance of As fractions were in the order exhibit various mechanisms to reduce As content of
of water-soluble < weakly adsorbed < specifically the affected sites viz. biosoption, bioaccumulation,
adsorbed < residual. It has been found that the biotransformation, biomethylation/ biovolatilization of
availability of arsenic in the paddy soils is highly and arsenic and tend to reduce its bioavailability to crops.
significantly correlated to pH, EC, available sulphur, Biostimulation is a strategy which can be adopted to
organic matter and available phosphorus of enhance the growth of these native arsenic remediating
contaminated soil. Bioavailable soil arsenic fraction fungi under As stressed soils, which are also nutrient
increased with increasing available sulphur-sulfate, EC deficient. Within this strategy organic matter
and organic matter contents and decreased with amendments are added to enhance the performance
increasing P and Fe content. This study provides new of the soil fungi leading to the better arsenic removal.
insight to arsenic bioavailability in soil and this may lead Rice straw, biochar and cow dung manure are some
with reference to develop an effective soil-specific of such amendments that have been found effective in
remediation strategy to arsenic contaminated regions stimulating the growth of arsenic remediating fungi
of India. leading to better removal of arsenic in contaminated
Key words: Arsenic, Bioavailable fractions, Soil property, matrices (water and soil) compared to unamended
Groundwater, Plant. matrices.
Key words: Arsenic, Bioremediation, Biostimulation, Fungi,
SVI/P-17 Nutrients.
groundwater. There are some physicos-chemical them, inorganic forms of arsenic i.e. arsenate (AsV)
methods for arsenic reduction or immobilization in soil and arsenite (AsIII) are highly toxic to living organism.
but these methods have some limitations such as These inorganic forms of arsenic are easily taken up
generation of highly toxic waste, as secondary source by the plants. Arsenic contaminated water used for
of contamination and high cost. Therefore, there is need drinking, irrigation purpose and food preparation poses
of less expensive ecofriendly technologies like great threat to public health. In India, arsenic
bioremediation for arsenic decontamination. Arsenic concentration in ground water has been found ranging
in soil should be below 20 mg kg-1 as per FAO and for from<10 ppb to as high as 3192 ppb by several authors.
paddy grains it’s should be below 1 mg kg-1 of dry Environmental protection agency (EPA) has reduced
weight (WHO). Arsenic contamination in soil the maximum contamination level for arsenic in drinking
environment has now gained concern of scientific water from 50 ppb to 10 ppb. In soil background arsenic
community because of its accumulation in rice (above concentrations has been reported from 1 to 40 ppm
its permissible limit) a major staple crop especially in with a mean value of 5 ppm. Due to emerging problem
Asian countries. In this review, mycoremediation of arsenic accumulation in plants, soil contamination
(remediation by fungal biomass) has been reviewed has gained interest. Various plants species have different
for lowering and immobilizing arsenic concentration in mechanisms responsible for As (III) and As (V) uptake,
soil and ultimately its accumulation by plants. Various toxicity and detoxification in plants at physiological and
mechanisms have been reported based on fungal biochemical levels. Its chemistry is governed by
activity to immobilized arsenic through bioaccumulation, oxidation-reduction process, acid-base reactions,
redox reactions, volatilization, methylation, co- absorption-precipitation process, plant uptake and
precipitation and complexation or chelation of arsenic accumulation. Ultimately, such mechanisms for
from the contaminant sites. Among these mechanism reducing arsenic contamination provide the opportunity
methylation or methylation followed by volatilization to safe agriculture practices and hence safeguarding
have been found comparatively more effective arsenic the food supply. The use of bioremediation is a potential
decontamination process. Since fungi have advantage method to reduce arsenic concentration in contaminated
over other microbes due to having comparatively larger areas of Gangetic plains of Uttar Pradesh.
biomass, hyphal network, metabolic competence for Bioremediation process in this regards in an option that
metals, and longer life-cycle. Therefore, methylation offers the possibility to destroy various contaminants
of arsenic using soil fungi concluded as a potential using plants and microbes. The aim of this article is to
alternative to arsenic remediation from soil. review arsenic contamination in soil and its mechanisms
Key words: Arsenic, Mechanism, Mycoremediation, Review, for removal from the contaminated source by the
Soil. potential application of plant and microbes.
Key words: Acid-base reaction, Arsenic toxicity,
SVI/P-19 Bioremediation, Detoxification, Soil contamination.
earth’s crust and is a potential human carcinogen. are more toxic than its organic forms, which is
Geogenic arsenic contamination in groundwater is predominant in arsenic contaminated groundwater. The
prevalent in several countries of the world viz; presence of arsenic in groundwater has been a major
Bangladesh, China, USA, India etc., which is posing public health concern in many countries, such as
serious health hazards such as skin lesions, Bangladesh, India, Thailand, China, USA and Nepal.
carcinogenic aliment in human beings. Several Human exposure of arsenic occurs via arsenic polluted
technologies have been applied for mitigation of arsenic water, through ingestion of arsenic contaminated food
contaminated water such as filtration, coagulation, produces. It may cause ‘arsenicosis’ and several
membrane separation, ion exchange, etc. But these carcinogenic diseases. Biosorption is a treatment
methods are less effective, requiring high material process involving biological oxidation-filtration-sorption
costs, high energy requirements, and generation of processes. Fungi have been recognized as promising
sludge. To overcome these limitations, nanotechnology low-cost adsorbents for arsenic removal from arsenic
has been emerged as a proficient strategy to treat contaminated water. Arsenic removal using fungal
arsenic contaminated water in a more specific and biomass have been found effective after modification
accurate way using biogenic nano materials. of fungal biomass by various modifying agents. There
Nanomaterials (NMs) have higher surface volume due are various modifying agents such as iron oxide, ferric
to their nano size and possessing other relevant chloride, and surfactants (such as hexadecyl-trimethyl
biologically properties. NMs which are being ammonium bromide and dodecylamine). Most of the
synthesized using biological organisms (e.g. microbes studies using fungal biomass (Penicillium
and plants) are referred as biogenic nanoparticles. The purpurogenum, Aspergillus niger, Aspergillus
biogenic synthesis of NMs mainly involves bioreduction fumiguts and Aspergillus flavus) revealed that
and bioprecipitation by polyphenols, peptides, amino modification with different salts of iron have potential
acids and other bioactive compounds obtained from to remove arsenic from the drinking water as well as
living organisms. In the present study, promises and from waste water. Different surfactants as modifying
challenges for synthesis of biogenic nanomaterials from agents have been used for arsenic removal from waste
bacteria, fungi, yeast, algae and plants have been water. Fungi have a specific metal-binding proteins and
discussed to synthesize various size and shape of peptides (such as metallothioneins). Iron oxides have
metallic nanoparticles. Of these biogenic NMs, our aim been reported to have relatively high affinity for several
to apply the plant-mediated synthesis of biogenic inorganic oxyanions. Easy recovery of iron from the
nanomaterials and their potential applications in saturated arsenic loaded fungal biomass by magnetic
removing arsenic from contaminated water as being a separation process added the advantage of using iron
feasible, efficient and ecofriendly. modified fungal biomass.
Key words: Arsenic, Biogenic, Nanotechnology, Key words: Aqueous solution, Arsenic removal, Biosorption,
Bioremediation, Contamination. Fungal biomass, Iron oxide.
SVI/P-21 SVI/P-22
to remove As below WHO permissible limits for region of Ghaghara basin is less arsenic polluted being
drinking water (10 µg l-1) under field conditions in As maximum (4.7ìgl-1) in Mugu district of Nepal. In these
affected areas. districts cereal crops like rice and animal based food
Key words: Arsenic, Arsenic Ground water problem, are the main As exposure to human due to transfer of
Treatments plants. As to higher tropic level through food chain
contamination. The many local inhabitants of these
SVI/P-26 districts are suffering from different disease induced
by arsenic accumulation in their body. The highest
Contamination of Arsenic in Ghaghra amount of arsenic has been detected in their nails
Basin: A Potential Thread to Local followed by hairs and urine.
We found some temporal variations in weather and (86.90%), nitrogen (64%.16), potassium (72%), total
monsoonal events, while studying present weather organic carbon (202%), chlorine (35%), phenol
conditions using these bio indicators as weather (38.46%), hardness (51.48%) and floride (64.90%).
forecasting tool and comparing them with pre described The microalgae also demonstrated high reduction in
theories given by ancient Indian Rishis. Research need the percentage of different heavy metals in the range
and priorities emphasized on- i. Identification and between 43 to 99% in continuous system. Waste water
inventorization of flora as bio indicator species to supported algal growth which exhibit number of
promote non-destructive study of climate change. ii. dependent and independent metabolism process of the
Establishment of bio indicator gardens for research and uptake and accumulation of heavy metal. Thereby with
educational purposes. iii. Awareness about the recent advances in scientific knowledge, sophisticated
importance of ancient Indian sciences among young techniques and environment awareness microalgae
researchers. could offer an elegant, environment friendly solution to
Key words: Bio indicators, Ancient Indian Science, Plants tertiary and quandary treatments of sewage water.
and animals, Weather forecast, Climate change. Furthermore, harvested algal biomass can be utilized
for several value added products development such as
SVI/P-28 bioenergy, pharmaceuticals, nutraceuticals, genetically
engineered products etc.
Bioremediation of Sewage using Two Key words: Phycoremediation, Biorefinery, Waste-water
Potential Microalgae treatment, Chlorophyceae.
of plant height, biomass and heavy metal accumulation the availability of heavy metals. Further, despite the
in different plant parts. The results indicated that fly heavy metal contamination, we observed a very
ash contains significant amounts of Mn (411 mg kg-1), complex and indifferent pattern of bacterial community
Fe (11083 mg kg-1), Cu (41 mg kg-1), Zn (27 mg kg-1) composition along the heavy metal contamination sites.
and toxic Cr (61 mg kg-1), Ni (40 mg kg-1), As (8 mg Overall, we found that c-Proteobacteria had been the
kg-1 ), Pb (4 mg kg -1 ) metals. Among the plants, most abundant bacterial community followed by
Saccharum spontaneum, accumulated highest amount Actinobacteria, Firmicutes, b-Proteobacteria and a-
of Cr (5 mg kg-1) and Ni (13 mg kg-1) in roots, while Proteobacteria. Commemorating all the results, we can
arsenic was found highest in the shoot of Pteris vittata infer that arsenic and other heavy metal contamination
(38 mg kg-1). After one year, the reduction in metal is deteriorating the soil quality and hence warrants
content of fly-ash was found maximum for Cu (65%) immediate attention of concerned soil scientist and
followed by As (64%), Cr (55%), Zn (46%) and Ni agronomists.
(35%), Fe (31%) and Mn (22%). Therefore, the study Key words: Arsenic, Paddy, Proteobacteria, Enzymes.
concluded that the Saccharum spontaneum, and Pteris
vittata are the most suitable plants to develop SVI/P-31
phytoremediation technique for decontamination of fly
ash dykes. Correlation of different parameters/results A Study on Carbon Sequestration
will be presented during the conference.
Potential of Chlorella ellipsoidea
Key words: Fly-ash, Heavy metals, Food chain
contamination, Phytoremediation.
Isolated from Domestic Sewage Water
with Inorganic Source of Carbon
SVI/P-30
Himangshu Sharma*, Mahdi Ahmed, Pampi
Arsenic Contamination Confines Sarmah and Jayashree Rout
Fertility Indicators of Paddy Soil Phycology and Lichenology Laboratory, Department of
Ecology and Environmental Science, Assam University,
Vijay Kant Dixit, Shashank Kumar Mishra, Sankalp Silchar, Assam (India) Pin-788011, Email: hshimangshu270
Misra, Arpita Singh, Puneet Singh Chauhan* @gmail.com, mahdiahmed438@gmail.com, mailtopampi@
rediffmail.com, routjaya@rediffmail.com
Division of Plant Microbe Interactions, CSIR-National
Botanical Research Institute, Lucknow-226001, INDIA, The potential of microalgae have been well
Email: puneetnbri@gmail.com recognized for its efficient carbon fixation and
Emerging environmental issues related to heavy contribution in substantial biomass production. The
metal contamination in rice draw great concern about present paper highlights the carbon sequestration
the soil quality of paddy farming lands irrigated with efficiency of a freshwater algal species viz. Chlorella
groundwater. Investigating the functioning of soil ellipsoidea isolated from domestic sewage water of
microorganisms exposed to heavy metal contamination Cachar district, Assam (India). Growth response of
is imperative for agricultural soil manipulations. The the aforementioned species was monitored under
current study accentuates the influence of heavy metals varying concentrations of nitrate (1.0, 1.5, 2.0 g l-1),
on microbial activity and community composition in phosphate (0.03, 0.04, 0.05 g l-1) and CO2 (g) (20, 40,
arable soil of West Bengal State of India. The result 60, 80,100 ml min-1). The value of pH ranges from
revealed that the fertility indicators (activity of all soil 5.92 to 9.93. The maximum chlorophyll a content and
enzymes) and growth-limiting factors (soil N and P) cell density in case of Chlorella ellipsoidea was
were negatively correlated with the heavy metal stress estimated to be 4.79 µg ml-1 and 984 cells/ml×104 during
except the soil total organic content which demonstrated study period respectively. The maximum protein and
significant positive correlation with the heavy metals. carbohydrate content was observed to be 44.99 µg ml-
1
In case of functional diversity of soil, all the considered and 118.97 µg ml-1, respectively. The highest biomass
diversity indices exhibited no specific pattern along with productivity and CO2 fixation rate was found to be 64.3
(mg l-1 day-1) and 120.89 (mg l-1 day-1), respectively. Key words: Wastewater, Emerging chemicals,
This microalga seems to be a promising candidate for Phytoremediation technology, Constructed wetland.
effective carbon sequestration ability and in general,
this study may be advanced further for strategic SVI/P-33
optimization of higher microalgal biomass yield, lipid
production and other value added products.
Strategies for Arsenic Reduction in
Key words: Carbon sequestration, Microalga, Chlorella
Crops for Sustainable Environment and
ellipsoidea, Sewage. Agriculture
SVI/P-32 R.D. Tripathi 1*, R. Chauhan1 , S. Awasthi 1, P.K.
Singh, M. Shri1, P. Verma1, S. Dwivedi1, S. Mishra2,
Removal of Emerging Contaminants P.K. Srivastava1 , B. Adhikari 3, P.K. Trivedi 1 and
of Municipal Wastewater through D. Chakrabarty1
Sustainable Phytotechnologies 1
CSIR-National Botanical Research Institute, Rana Pratap
Marg, Lucknow-226001, Uttar Pradesh, INDIA; 2DDU
Gurudatta Singh* and Virendra Kumar Mishra University, Gorakhpur, Uttar Pradesh, INDIA; 3Rice Research
Station, Department of Agriculture, Chinsurah, Hooghly,
Institute of Environment and Sustainable Development, West Bengal, INDIA, E-mail: tripathird@gmail.com
Banaras Hindu University, Varanasi-221005, INDIA, Email:
gurudatta.singh2@bhu.ac.in Arsenic is a widely recognized human carcinogen
and declared as non-threshold toxic pollutant
This study mainly discuss the removal of worldwide. The most common routes of arsenic
emerging chemicals in wastewater through exposure to humans are the usages of contaminated
phytotechnologies. The occurrence of emerging water for drinking and irrigation in agricultural field.
contaminants or newly identified contaminants in This leads to arsenic contamination in crops and
municipal wastewater is of continued concern for the vegetables grown on such soil eventually contaminating
health and safety of public. The design parameters of the food chain. In a regime where cultivation of GM
the reported constructed wetlands including the physical crops are not encouraged, selection of low grain arsenic
configuration, hydraulic mode, vegetation species, and cultivars of crops through breeding process, needs to
targeting pharmaceuticals were summarized. The be extensively explored. Rice being a cereal consumed
removal efficiencies of pharmaceuticals under different by half of world’s population, needs special attention.
conditions in the wetlands were evaluated. In addition, Through breeding and selection, a rice cultivar CN1794-
the importance of the three main components of 2-CSIR-NBRI was developed. This rice cultivar while
constructed wetland (substrate, plants and microbes) accumulating low As maintains higher level of
for pharmaceutical removal was analysed to elucidate micronutrients such as Fe, Cu, Zn, Se and Ni.
the possible removal mechanisms involved. The Application of arsenic tolerant bacterial strains
compounds were extracted from municipal wastewater decreased arsenic uptake in shoots and increased
samples by solid phase extraction and analyzed by ultra- stabilization of arsenic in root zone of rice plants, can
performance liquid and gas chromatography couple with also be a cheap and safer strategy. In order to restrict
tandem mass spectrometry (UPLC, GCMS, MS). As in the rice roots a transgenic approach has been
Most of the target compounds were detected with the followed through expression of phytochelatin synthase
concentration of 4.4 ng L -1 to 6.6 µg L -1 . (PCS), CdPCSI, from Ceratophyllum demersum, an
Phytoremediation technology through constructed aquatic As accumulator plant. Rice transgenic lines
wetland was used to remove target compounds such showed enhanced accumulation of As in root and shoot.
as sulhamethox azole and tetracycline, with the removal However, all the transgenic lines accumulated
efficiency of 17% to 98% for sulhamethox azole, and significantly lower As in grain and husk in comparison
12-80% for tetracycline was achieved. Constructed to non-transgenic plant. The higher level of PCs in
wetlands had better efficiency to remove emerging transgenic plants relative to non-transgenic presumably
contaminants from wastewater. allowed sequestering and detoxification of higher
amounts of As in roots thereby restricting its Stable isotopes occur naturally in environment and
accumulation in grain. Another approach could be of have emerged as promising environmental tracers.
As volatilization through genetically engineered They are safe and non-radioactive, and do not decay,
Arabidopsis thaliana with arsenic methyltransferase thus are suitable natural environmental tracer in
(WaarsM) gene from one of the fungal strain understanding various environmental processes and
Westerdykella auantiaca (MTCC10845), isolated pollutant dynamics in environment. Stable isotopes have
from arsenic-contaminated sites. The WaarsM been used in understanding biogeochemical cycles.
transgenic A. thaliana plant showed enhanced Stable isotopes have been used in different biological
tolerance to AsV and AsIII, compared to wild-type and ecological studies. It can be used to understand
(WT) plants. WaarsM expressing transgenic plants fundamental processes in the biology and ecology of
evolved significant amount of volatile arsenicals (mg-1 arthropods, which range from nutrition and resource
fresh weight) after 48 h of exposure. Further, WaarsM allocation to dispersal, food-web structure, predation,
gene expressed in rice was able to convert toxic feeding behavior, mating, etc. Compound-specific stable
inorganic arsenicals to methylated arsenic species, isotope analysis (CSIA) has been used to study the
therefore, reduce arsenic accumulation in rice grains. fate of various groundwater pollutants, such as
In response to arsenic treatment in hydroponics, monoaromatic and polyaromatic hydrocarbons, MTBE,
WaarsM expressing transgenic lines showed a marked chlorinated ethenes, perchlorate, nitrate, and chromium
increase in arsenic resistance and reduced its (VI). CSIA has also been used in monitoring diffuse
accumulation compared to NT. Also, WaarsM pesticide pollution in river. Metal stable isotope
expressing transgenic Line 1 evolved c.a. 157 ng and signatures can be used as tracers to get a deeper
c.a. 43 ng volatile arsenicals (mg-1 fresh weight) after understanding of environmental geochemistry and to
72 h of exposure to 25 ìM AsIII and 250 ìM AsV, deduce information about composition and history of
respectively. Transgenic line 1, grown in soil irrigated environmental samples and understanding other
with arsenic-containing water accumulates about 50% environmental processes like redox process,
and 52% lower arsenic than the NT in shoot and root, complexation and organic matter binding, biological
respectively; while arsenic concentration in polished cycling, etc. This paper presents a review on potential
seeds and husk of the transgenic line was reduced by use of stable isotopes in biological, ecological and
52% compared to NT. Thus, the present study
pollution studies.
demonstrates that the expression of WaarsM in rice
induces arsenic methylation and volatilization, provides Key words: Stable isotopes, Compound-specific stable
a potential strategy to reduce arsenic accumulation in isotope analysis (CSIA), Environmental processes, Pollutant
rice grain. Suitable combined biotechnological and dynamics.
microbe plant interaction approaches may lead to safer
SVI/P-35
levels of arsenic in the food crops grown in the arsenic
contaminated soils.
Lichen Use as a Biomonitoring Tool for
Key words: Arsenic, Methyl transferase, Microbes,
Arabidopsis thaliana.
Assessment of Air Quality
through use of plants, which tolerate and accumulate takes place or not by geophysical signatures.
high levels of heavy metals. Microorganisms alter the chemical and physical
Key words: Phytoremediation; heavy metals, properties of soil during its metabolism process. These
phytoextraction, accumulation, translocation changes lead to altered geophysical signatures. Hence,
geophysical methods being non invasive, cost effective,
SVI/P-37 less time consuming and less expensive, provides an
opportunity for using them as an innovative approach
Bioremediation and Monitoring of 2,4- for getting high density spatial and temporal information.
D in Contaminated Soil of Vidarbha This information can be used in mapping contaminated
soil and monitoring its bioremediation.
using Non-Invasive Geophysical Tools:
Key words: Bioremediation, Geophysical, 2,4-D,
A Novel Approach Bioaugmentation, Biostimulation.
chemical parameters of groundwater for pre and post cytological analysis. The cytogenotoxicity of fertilizers
monsoon variations in Gorakhpur district. For the study was assessed by markers- mitotic index and
of groundwater total twenty samples are collected from chromosomal aberrations during the different stages
different sampling sites during two season winter (post of mitosis. Results will be discussed.
monsoon ) and summer (pre monsoon). The physic- Key words: Biomarkers, Cytogenotoxicity, Allium cepa,
chemical parameters like temperature, pH, EC, TDS, Genotoxic.
Total hardness, turbidity, chloride, alkalinity, fluoride,
nitrate were analyzed. It has been found that the value SVI/P-46
of parameters is higher in pre monsoon season than
post monsoon season. The purpose of this study is to Pyrenocarpous Lichens as an
verify the quality of ground water. Indicator of Ecological Continuity in
Key words: Ground water, Total Hardness, Pre monsoon, Forests of Manipur and Nagaland
Post monsoon, Sampling sites.
SVI/P-49 SVI/P-50
Polycyclic aromatic hydrocarbons (PAHs) are cause pathological stress, false satiation, reproductive
ubiquitous environmental pollutants found in soil and complications, blocked enzyme production, reduced
sediments, groundwater and atmosphere. Many PAHs growth rate and oxidative stress in organisms. MPs
and their epoxides are toxic and mutagenic / can also potentially transfer chemicals adsorbed on their
carcinogenic in nature. This investigation evaluates the surface in the food chain. Currently, knowledge and
degradation ability of three bacterial strains, such as data on the accumulation and effects of microplastics
Pseudomonas aeruginosa PSA5, Rhodococcus sp. in freshwater and terrestrial systems is much limited
NJ2 and Cronobacter sp. PSM10, isolated from than that in marine systems. In this context the
petroleum sludge containing 500 ppm of fluorine in occurrence of microplastic pollution was investigated
mineral salt medium. A maximum degradation of 79% in the sediment and the mussel samples from the Ganga
was attained by PSA5, followed by PSM10 (71%) and river at twelve sites in Varanasi. The samples were
a minimum was recorded for NJ2 (68%) after 7 days processed and measured for microplastic through alkali
of incubation. Induction of catabolic enzymes of two digestion and density separation. Results suggest the
major pathways involved in the formation of catechol presence of variety of microplastics including fibres
and protocatechuate as intermediates was also and fragments in the sediments as well as tissues of
examined. Between the two pathways, enzymes mussels. Based on our results, it is evident that
associated with protocatechuate pathway were more microplastic pollution in River Ganga at Varanasi is
induced than that of catechol pathway. Besides, statistically significant and that freshwater mussels due
degradation kinetics and half life of anthracene were to their extensive filter-feeding activity are exposed to
also determined to find out the degradation pattern these microplastics directly present in the water column
caused by bacteria. Biosurfactant production, cell which may thereby enter our food chain, however
surface hydrophobicity and emulsification index of detailed studies are needed to prove the hypothesis.
anthracene, which facilitate anthracene degradation, Key words: Fresh water pollution, Food chain, Ganga river,
were also determined in all bacteria to substantiate their Microplastics, Varanasi.
degradation potential.
Key words: Fluorene contamination, Anthracene, Catabolic, SVI/P-53
Degrading microbe.
Fluoride: Pollution and Mitigation
SVI/P-52
Gayatri Singh 1 *, Geetgovind Sinam 1 , Babita
Microplastic Pollution in River Ganges Kumari1 , Kriti1 , Mahiya Kulsoom2 and Shekhar
at Varanasi: A Case Study Mallick1
1
Plant Ecology and Climate Change Science Division, CSIR-
Vyomendra Chaturvedi* and Kavita Shah National Botanical Research Institute, Lucknow-226001, U.P.,
INDIA; 2Department of Environment Science, Babasaheb
Environmental Biotechnology Laboratory, Institute of Bhimrao Ambedkar University, Lucknow-226025, INDIA,
Environment and Sustainable Development, Banaras Hindu Email: gayatrisingh.15@gmail.com
University, Varanasi-221005, INDIA, Email: vyomendra.
chaturvedi04@bhu.ac.in, kavitashah@bhu.ac.in Fluorosis is the most prevalent groundwater-
related disease in India, the most severely affected
Microplastics (MPs), a manmade litter having
country worldwide. The desirable and the maximum
particle size <5 mm, have recently been identified as
permissible limits of the flouride concentrations in the
an emerging global concern which affects aquatic
drinking water are 0.6 mg l-1 and 1.0 mg l-1, respectively
organisms and human beings. The small sizes of MPs
(WHO). In Uttar Pradesh Unnao is one of the most
make them easily available for ingestion by a wide
fluorosis affected district. Few studies have previously
variety of organisms such as zooplanktons, bivalves,
reported the F- level in the drinking water in Unnao
mussels, shrimps, oysters, copepods, worms and by
region, which ranges between 0.8-13.9 mg L-1. There
organisms from higher trophic levels as fish, marine
are various physical, chemical and biological methods
mammals etc. Ingestion of these tiny particles can
mentioned to defluoridation of drinking water. To Natural wetland act as a biofilter, removing sediments
mitigate this problem, there is an urgent need to and pollutants such as heavy metal from water, and
understand the current status and brief knowledge of F-. constructed wetland is designed to emulate these
Key words: Fluoride, Unnao, Defluoridation. featureas. Constructed wetland is built by human.
These constructed wetlands are primarily built to clean
SVI/P-54 up waste water and create wild life habitat. Constructed
wetland naturally filters and treats waste water and
Ganga Water Pollution Management are often less expensive than traditional treatment
plants.
by Green Clean Technology and
Key words: Ganga, Water pollution, Green technology.
Outreach Programmes
SVI/P-55
Mridul Kumar Shukla
Outreach Programmes, CSIR-National Botanical Research Estimation of Arsenic in Rice Grains
Institute, Rana Pratap Marg, Lucknow-226001, INDIA, Email:
mridul_shukla@rediffmail.com; mridulcsir0101@gmail.com
and Rice-Based Food Products in
Gangetic Region
The Ganga river has been considered as the most
sacred river of India in Ved Puran. It is called as “Ganga Nidhi Tyagi and Sudhakar Srivastava*
Maa” or Mother Ganga or Ganga Ji. People of world
believe that a bath in the holy water of Ganga washes Institute of Environment and Sustainable Development,
all the past sins of a person. It has a symbol of purity, Banaras Hindu University, Varanasi-221005, INDIA, Email:
sudhakar.iesd@bhu.ac.in, sudhakar.srivastava@ gmail.com
and for Mokshh . It has long history of reverence in
India and it is often called Holy Ganga. The total length Arsenic (As) is a toxic carcinogenic element
of the river is about 2510 K.M.; one of the largest which poses threat to millions of lives around the globe.
groups of human population belts on earth is built around The element has already entered the food web as many
the Ganga. Its originated from Bhagirathi from Gangotri of the crops and animal products are reported to have
glacier in the Uttarakhand. The basin drains eight As in high concentrations. The range of health impact
states, H.P., Panjab, Haryana, U. P., Rajasthan, M. P., of As is from skin disorder to cancer. In few reports,
Bihar, West Bengal and Delhi. The level of pollution in As exposure has been linked to diabetes mellitus and
the Ganga River has reached alarming proportions. Two adverse pregnancy outcomes (APO) too. The present
types of point sources like, surface drains carrying study was conducted to analyze As content in rice
municipal sewage or industrial effluent, pumping stations grains of market sold rice varieties and rice-based food
and sewage system from industries etc are found. The products (laiyya, chuda, and snacks) in Gangetic region.
point source can be measured. Second is non point The cities covered in Gangetic region include Kanpur,
source which cannot be measured such as runoff Allahabad, Varanasi, Balia, Patna and Kolkata. In
agriculture field carrying chemical and fertilizers, run addition, Jabalpur was taken as a control area which is
off from area used for dumping of solid waste and not reported to have As contamination. The samples
open defecation, mass bathing, floral offerings etc. were oven dried, crushed and digested in nitric acid.
According to study nearly 89 million liter of sewage is After this, the samples were analyzed for As using
disposed into Ganga from 12 municipal towns that fall Atomic Absorption Spectroscopy. The results show
along its route till Haridwar, where the amount of presence of As in the samples that varied from variety
sewage disposed in the river increase during the Char to variety, and in different areas. The results are
Dham Yatra season. A constructed wetland is an discussed with possible health impacts considering
artificial marsh or swamp, created for anthropogenic standard As limits in rice grains, and in food products,
discharge such as waste water storm water runoff or rice consumption rates and As consumption limits for
sewage treatment and as a habitat for wild life, or for humans.
land reclamation after mining or other disturbances. Key words: Arsenic, Rice, Gangetic region.
ENPs may also be tested with microalgae in municipal characterized for different plant growth promoting
wastewater, to assess the effect on biomass yield as attributes viz. indole-3-acetic acid, phosphate
well as pollutant removal. If successful, the proposed solubilization, siderophore production. Assessment of
process will serves as a role model for bioremediation arsenic stress amelioration using rice as a host plant
coupled biofuel production using microalgae. under green house condition is under progress.
Key words: Engineered nano-materials, Biofuel, Key words: Arsenic, Yeast, Arsenite oxidase, Reductase
Bioremediation, Microalgae, Wastewater. activity.
SVI/P-58 SVI/P-59
SVI/P-60 SVI/P-61
Session VII
Plant Response to Environmental Pollution
Nitric oxide (NO), a signal molecule play vital agricultural areas has raised an alarming situation. Rice
role to provide tolerance to abiotic stress in plants by is an important food crop of India which feeds about
interplay with reactive oxygen species, and thus half of the population, but presence of As in
promoting their growth and development. Arsenic (As) environment has lead to its accumulation in edible plant
is a toxic metalloid and its contamination found in crop parts, which effects human and animal health. In this
plants, mainly rice which is an important diet for millions study, we have analyzed the effect of AsIII on two
of people. In this study 7 days old hydroponically grown rice varieties- Varsha and Jaya. Rice seeds were
rice seedlings were exposed to As(III) (150 µM), NO exposed to AsIII (150ìM) for 7 days to observe seed
(100 µM), As(III)+NO for 48 h and control (without germination and relative As-injury rate while
metal).We observed NO mediated alteration on hydroponically grown 12 days old seedlings were
physiological, biochemical and stress related exposed to AsIII (150ìM) for 96 h to study the changes
parameters along with auxin transporter PIN genes (Os at biochemical and molecular levels. Changes in
PIN1a, Os PIN1b, Os PIN1c, Os PIN1d, Os PIN2, phenotypic characters, physiological parameters
Os PIN5a, Os PIN5c, Os PIN8, Os PIN9, Os (chlorophyll, protein), stress indicators (MDA and
PIN10b), expression under As stress. As exposure H2O2) along with accumulation of As and macro/micro-
reduced the overall plant growth, formation of lateral nutrients (Na, Mg, S, Mn, Fe, Cu and Zn) constituted
roots, chlorophyll and protein content and enhanced the biochemical approaches, while RAPD analysis was
the oxidative stress by increasing the level of antioxidant done to observe the changes at molecular level. WRKY
enzymes (SOD, CAT, APX, GR), and stress related TFs play a key role in plants during heavy metal stress,
parameters (cysteine, proline, MDA, thus an attempt has been made in this study to observe
H2O2).Supplementation of NO along with As reduced their expression analysis in response to AsIII toxicity
the accumulation of As in rice seedling, improved plant in both rice varieties. Our observations showed that
growth, lateral root formation, increased chlorophyll and seed germination, phenotypic and physiological
protein content, diminished the level of antioxidant parameters decreased, while stress indicators increased
enzymes and stress related parameters by reducing during AsIII exposure as compared to control. Nutrient
the ROS generation. Addition of NO also up regulates profiling results also demonstrated the negative effect
the gene expression of auxin transporters. Overall, NO of AsIII on both rice varieties. Further, results obtained
reduced the toxicity of As using various mechanism from RAPD band profiles and GTS were compatible
and provide tolerance to its stress in rice seedlings. to the above parameters indicating AsIII mediated
Key words: Arsenic, Nitric oxide, Oxidative stress, toxicity in rice varieties. Analysis of the WRKY
Antioxidant enzymes, ROS. expression profile indicated that these TFs play a role
in plant processes by regulating stress tolerant genes.
SVII/O-2 Key words- Arsenic, Rice, Nutrient profiling, RAPD, WRKY.
number of motor vehicles has been found to be their dispersal, impact on river ecology and ecosystem
positively correlated with increase in air pollution levels functions, despite the fact that such information are
in different urban areas of the world. Vehicular pollution important on which integrated river basin management
has resulted in various adverse and irreplaceable effects strategies can be keyed. In this study, we investigated
on plants, animals and human beings at various levels shifts in the pattern of ecological nutrient limitation and
ranging from biochemical to ecosystem levels. Plants, relative diatom assemblage as influenced by a point
being the true friends to all life on this earth have the source (Assi drain) input, and up- and downstream
capability to improve the quality of air by absorption of Varanasi urban influences along a 37 km stretch of the
gases and particles. Various plant parts viz., roots, Ganga River for three consecutive year(March 2013
leaves and pollen generally get exposed to different to February 2016).We found point- and non-point
types of soil and air pollutants. The leaves show sources of input shifts N:P:Si stoichiometry of the river
apparent symptoms like, necrosis and chlorosis and toward N and Si limitation consequently altering the
likewise pollen grains also show various symptoms that relative diatom assemblages with increased abundance
can be assessed to measure the type and extent of of P favoured species such as Cyclotella
pollution. Pollen grains can provide important meneghiniana, Cocconei splacentula, Melosira
information about the biological impacts and can act varians and Hyalodiscus laevis downstream city. The
as sensitive bio-indicators of air pollution. Air pollutants dynamic fit function showed significant positive
like suspended particulate matter (SPM), SOX, O3, NOX, correlation of C. meneghiniana, C. placentula and
HF and heavy metals (Pb and Cr) led to reduction in Fragilaria intermedia with N: P (R 2= 0.99-0.85;
pollen grains germination, pollen tube length, size and p<0.001) and with Si: P ratio (R2= 0.99-0.83; p<0.001)
viability; pollen abortiveness; distorted pollen shape and reflecting their P favoured nature. Excessive growth
pollen tectum. The present paper is an attempt to review of such exploitative species replaced less adapted
summary of some literature on the effects of air pollution diatoms such as Aulacoseira granulates, Cymbella
on pollen of different plant species growing along affinis and Craticula halophila. If excessive P loading
roadsides/ traffic stressed areas. is continued, as expected in future, it will lead to high P
Key words: Motor vehicles; pollen grain; Vehicular pollution; condition and consequently a niche based shift in diatom
Bio-indicators. species composition. The study has relevance in
understanding interactive effect of diatom shift under
SVII/O-4 N and Si limiting conditions to restore the river property
as buffering agent and provides important cues for
Distributional Pattern of Diatom further study for river rejuvenation.
Assemblages in the Ganga River: Key words: Diatom, N: P: Si stoichiometry, Ganga River, Land
use, Point source, Water pollution.
Impact of Nutrient Enrichment and
Limitation SVII/O-5
Amita Yadav 1 *, Jitendra Pandey 1 and Usha Effect of Marble Slurry Dust on the
Pandey 2 Proline Content of Callistimon
1
Ganga River Ecology Research Laboratory, Environmental lanceolata and Albizialebbec Trees
Science Division, Centre of Advanced Study in Botany,
Institute of Science, Banaras Hindu University, Varanasi-
Vibha Khanna
221005, INDIA; 2Department of Botany, Faculty of Science
and Technology, Mahatma Gandhi Kashi Vidyapith, Varanasi Samrat Prithviraj Chouhan Government College, Ajmer,
221002, India, Email: amita0804@gmail.com, Rajasthan, INDIA, Email: vibhaajmer@yahoo.co.in
jiten_pandey@rediffmail.com, usha_pandey28@
yahoo.co.in
Free proline accumulation in the various plant
parts in Angiosperms has been considered as one of
The Ganga River still lacks systematic data the most widespread stress induced response. So, its
evaluating point- and non-point sources of nutrient input, measurement is an excellent stress detector. Increase
in free proline contents have been recorded under water accumulation in some higher plants as well as in arsenic
and osmotic stress. The accumulation of free proline hyper-accumulator Pteris vittata (Chinese Brake fern).
depends on the type and intensity of stress. The During present study the formation of low molecular
Callistimon lanceolata and Albizia lebbec trees weight thiols were investigated in a common native
growing in the green belt developed on the embankment fern Ampelopteris prolifera which was luxuriantly
of the Marble Slurry Dumpyard at Kishangarh (Ajmer) growing on fly ash (FA) dumping sites. In order to
are subjected to continuous suspended slurry dust which observe its toxic metal- induced adaptation, a pot
gets settled on various plant parts including the experiment was set up in three sets of FA amendments
phylloplane. The texture, thickness and orientation of viz. first set of 100% FA, second set of 50% FA and
leaves and the canopy architecture of the tree species 50% garden soil and third set of 100% garden soil only,
influence the dust holding capacity, which in turn treated as control. In this study, the synthesis of total
regulates the proline content of the plant. Availability thiols and acid-soluble thiols in Ampelopteris prolifera
of Slurry dust in the soil also leads to osmotic stress was investigated under fly-ash exposure. The strong
which in general enhances during rainy season when and positive correlation between heavy metal
the dust present on the trees as well as in the concentration and acid soluble thiols in leaf fronds
atmosphere is washed down into the soil. This exposes suggests that these thiols play pivotal role in metal
the vegetation to water stress and leads to accumulation detoxification. In addition to cystein and glutathione,
of proline in various plant parts particularly the leaves. an unidentified thiol was observed in plants grown on
Proline accumulation is as an adaptive response to fly ash, which was not found in the control. Our study
stress. It acts as an osmolyte and antioxidant, thus suggests that the thiols are synthesized due to metal
helping plants to maintain the survival of cell turgor. In stress in ferns grown on fly ash and these compounds
the present course of study, higher level of proline has (thiols) are helpful in metal detoxification in ferns and
been reported during winter months as well as during thus provide metabolic adaptation to the plant against
the rainy season. The former may be attributed to the toxic fly ash.
greater accumulation of suspended slurry dust on the Key words: Power plants, Fly ash, Heavy metals,
arial plant parts while higher level of proline during Pteridophytes, Thiols, Metal detoxification.
monsoons indicates the stress induced by the soil and
ground water pollution. Of the two tree species under SVII/O-7
consideration, proline content in plants exposed to
pollution was higher in Albizia lebbec as compared to Effects of the Desert Barriers in the
that in Callistimon lanceolata. Genetic Structure of Laperrine’s
Key words: Albizia lebbec, Callistimon lanceolata, Marble Olive, from the Central Saharan
Slurry, Seasonal variations, Stress, Pollution, Proline.
Mountains
SVII/O-6
Djamel Baali-Cherif 1,2 *, Guillaume Besnard 3 ,
Fly Ash-Induced Thiol Synthesis and Fabien Anthelme4 and Med-Salah Abdellaoui5
Metabolic Adaptation in Fern Searcher LRZA/USTHB BP 44 16000 Algiers-Gare, ALGERIA;
2
Ampelopteris prolifera (Retz.) Copel. Teacher ENSA (ex INA) El-Harrach, ALGERIA; 3CNRS,
Laboratory of Evolution and Biologic Diversity, UPS
Toulouse, FRANCE; 4IRD Montpellier (France), University
Alka Kumari1* and Ashu Gulati2 of Bamako, NIGER; 5Searcher INRF station, Tamanrasset,
1
Department of Botany, University of Lucknow, Lucknow- Hoggar, ALGERIA, Email: bacherdj@yahoo.fr
226007, INDIA; 2NPCPD Division, CSIR-Institute of
The Laperrine’s olive (Olea europaea subsp.
Himalayan Bioresource Technology, Palampur-176001, H.P.,
INDIA, Email: kumarialkasanjay@gmail.com
laperrinei) persists in the central Saharan mountains
from Algeria, Niger and Sudan. Populations of this tree
Thiol containing compounds have been reported are locally threatened of extinction due to climatic
to play an important role in metal detoxification and changes and human activities, particularly in Niger and
1
Algeria. In order to study the spatial genetic structure Department of Agronomy, Faculty of Agriculture, Sher-e-
and the dynamic of laperrinei populations, we sampled Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-
trees in four isolated massifs [Tassilin’Ajjer and Hoggar 1207, BANGLADESH; 2Department of Agriculture, Faculty
(Algeria), Tamgak and Bagzane (Niger)]. Two hundred of Science, Noakhali Science and Technology University,
Noakhali-3814, BANGLADESH; 3Department of Agricultural
and seven genets were identified based on nuclear
Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural
microsatellite polymorphism. Phylogenetic University, Sher-e-Bangla Nagar, Dhaka-1207,
reconstruction based on plastid DNA data supported a BANGLADESH; 4Laboratory of Plant Stress Responses,
maternal origin of laperrinei populations in South Department of Applied Biological Sciences, Faculty of
Algeria, where a higher allelic richness (on both nuclear Agriculture, Kagawa University, Miki-Cho, Kita-Gun,
and plastid loci) was also observed. Based on nuclear Kagawa 761-0795, JAPAN; 5Department of Plant Pathology,
microsatellite data, we revealed two levels of structure Faculty of Agriculture, Sher-e-Bangla Agricultural University,
using a Bayesian method: the first one allowed Sher-e-Bangla Nagar, Dhaka-1207, BANGLADESH; 6Citrus
recognition of most of individuals from Nigerien and Research Station, Bangladesh Agricultural Research
Algerian massifs whereas the second one showed a Institute, Jaintapur, Sylhet, BANGLADESH; 7Department of
Horticulture, Faculty of Agriculture, Sher-e-Bangla
less marked differentiation among the two populations
Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207,
from Niger and Algeria. These results support that BANGLADESH, Email: mhzsauag@yahoo.com
desert barriers may have greatly limited long distance
gene flow. Spatial genetic structure was then Nickel (Ni) is an essential plant nutrient but very
investigated within populations. We showed that toxic to plant at supra-optimal concentration and causes
genetic coefficients were significantly correlated to inhibition of seed germination, growth, and development
geographical distance within Nigerien populations but of plants. The present study investigates the possible
this was not observed within Algerian massifs. We mechanisms of Ni tolerance in rice seedlings by
discussed that historical factors and habitat exogenous application of silicon (Si). Hydroponically
heterogeneity may explain the differences observed grown 13-day-old rice (Oryza sativa L. cv. BRRI
for the allelic richness and the spatial structure within dhan54) seedlings were exposed to 0.25 mM and 0.5
population. Lastly, based on our results, we discussed mM NiSO4.7H2O alone and combined with 0.50 mM
on possible strategies for the conservation of Na 2SiO3 for 3 days to investigate the effect of Ni
populations of subspecies laperrinei. toxicity as well as the role of Si revoking the stress.
Nickel toxicity caused oxidative stress by
Key-words: Conservation, Microsatellites, Olive tree, Plastid
overproduction of reactive oxygen species and
DNA, Saharan mountains, Threatened species.
methylglyoxal in seedlings of rice that created higher
SVII/O-8 lipid peroxidation, and membrane damage. Nickel stress
also reduced growth and biomass, leaf relative water
Silicon-Induced Antioxidant Defense, content, and chlorophyll content of seedlings. However,
non-protein thiol (NPT) content, phytochelatin and
Methylglyoxal Detoxification and proline content increased in Ni-stress. In contrast, the
Metal Chelation Works Coordinately Ni-stressed seedlings supplemented with exogenous Si
in Alleviating Nickel Toxicity in Oryza recovered from water loss, chlorosis, growth inhibition,
and oxidative stress. Silicon up-regulated most of the
sativa L. antioxidant defense components as well as glyoxalase
systems, which helped to improve ROS and MG
Mirza Hasanuzzaman 1 *, Md. Mahabub Alam 2 , detoxification respectively. Hence, these findings
Kamrun Nahar 3 , Sayed Mohammad Mohsin 4,5 , suggest that the exogenous application of Si can
M.H.M. Borhannuddin Bhuyan 1,6 , Khursheda improve the tolerance of rice seedlings to Ni-toxicity.
Parvin1,7 and Masayuki Fujita1
Key words: Silicon, Reactive oxygen species, Methylglyoxal,
Metal chelation, Nickel.
SVII/O-9 SVII/O-10
metals suggesting its role in heavy metal stress observed in foliar morphology and biochemical
response. Further, functional characterization of constituents of an economically important plant Arjuna
OsSul1;1 was carried out by developing Arabidopsis (Terminalia arjuna) and Morus (Morus alba). It is
transgenic lines to understand its role in abiotic stress interresting to note that around 3-4 times higher fluxes
conditions including heavy metals. No significant of SO4— were recorded on foliar at industrial site as
change in the growth was observed in wild type (WT) compared to residential site.Atmospheric dust was also
and transgenic plants under optimum nutrient medium, characterized for other major ions (F-, Cl-, NO3-, Na+,
however, significant increase in the germination rate, NH4+, K+, Mg++ and Ca++). The morphology of foliar
cotyledon emergence and root length was observed in through Scanning Electron Microscope (SEM)
the transgenic plants in comparison to WT under sulphur indicated a significantdifference in size of the stomatal
limiting condition. Apart from the heavy metals, pores and raptured guard cells at industrial site as
transgenic lines showed resilience towards different compared to residential site.Results ofbiochemical
abiotic stresses such as salt and drought under sulphur analysis showed that plant foliar at the industrial site
limiting conditions. Therefore, the study suggested the had relatively higher values of ascorbic acid, proline
role of OsSul1;1 in maintaining enhanced sulphur pool amino acid and lower values of total chlorophyll.
inside the cell and a significant role in providing tolerance Key words: Dustfall, Sulfate (SO4—) flux, Ascorbic Acid,
against various stress conditions. Proline amino acid, Chlorophyll, SEM.
Key words: Arabidopsis, Arsenic, Detoxification, Rice,
Sulphate transporter, Sulphur. SVII/O-14
plants exposed to 30 minutes of UV irradiation in both Key words: Salinity stress, Salicylic acid, Phenolic profiles,
Nephrolepis tuberosa and Microsorum alternifolia NaCl stress.
than control. There was significant decrease in protein
content in Nephrolepis tuberosa as compared to SVII/O-16
Microsorum alternifolia when irradiated to UV light.
From the results it is suggested that Nephrolepis
Ganga Water Pollution: A Potential
tuberosais more susceptible to UV-B than Health Threat to Inhabitants of Ganga
Microsorum alternifolia. Basin
Key words: Ultraviolet-B, Ferns, Chlrophyll, Carotenoid,
Protein. Sanjay Dwivedi*, Seema Mishra and R.D. Tripathi
Plant Ecology and Environmental Science Division, CSIR-
SVII/O-15
National Botanical Research Institute, Rana Pratap Marg,
Lucknow-226001, India, Email: drs_dwivedi@yahoo.co.in
Ameliorative Effect of Salicylic Acid
The water quality of Ganga, the largest river in
on Growth and Seedling Establishment Indian sub-continent and life line to hundreds of million
of Corn (Zea mays L.) Grown under people, has severely deteriorated. Studies have
NaCl Stress indicated presence of high level of carcinogenic
elements in Ganga water. We performed extensive
P.K. Singh review of sources and level of organic, inorganic
pollution and microbial contamination in Ganga water
Department of Botany, Udai Pratap Autonomous College,
to evaluate changes in the level of various pollutants in
Varanasi-221002, INDIA, Email: drpksinghupc@gmail.com
the recent decade in comparison to past and potential
In this changing environment plant have to face health risk for the population through consumption of
major threat in natural ecosystem. The major cause of toxicant tainted fishes in Ganga basin. The analysis of
stresses are heat, drought, salinity and cold stress. data showed that despite several Govt. plans to clean
Among these soil salinity is one of the most devastating Ganga, the amount of untreated waste water discharge
environmental stress which causes major reduction in into Ganga has increased by many folds in past four
productivity and quality via affecting physiological and decades resulting in high increase in microbial load and
biochemical function of plants like inhibit photosynthesis disturbed physicochemical parameters. The level of
through its effect on stomatal conductance absorption pesticides in Ganga water registered drastic reduction
of water and nutrients .Plant exhibit several mechanism in last decade (i.e. after the establishment of National
that can alleviate the deleterious effect of salinity under Ganga River Basin Authority (NGRBA) in 2009); still
changing environment. The aim of the present work the levels of some organochlorines are beyond the
was to investigate the accumulation of phenolic acids permissible limits for drinking water. Conversely the
in 2 weeks old maize (Zea mays L.) plants grown under inorganic pollutants, particularly carcinogenic elements
salt stress (0, 50, 100, 150 and 200 mM NaCl) in have increased several folds. Microbial contamination
presence and absence of 0.5 mM SA. The results has also significantly increased. These pollutants have
showed sever reduction in plant dry weight, leaf relative adversely affected the unique properties, such as self-
water content and photosynthetic pigments. Content purification, antibacterial and incorruptibility of Ganga
of total phenolics was increased by 64%, in the plants water as well as its fish fauna and fish health. The
after salt treatment. The exogenous application of SA high load of pathogens and chemical toxicants in middle
significantly alleviated the growth inhibition of plants and lower stretch of Ganga make the water unsuitable
caused by NaCl, and was accompanied by higher leaf for consumption or other domestic uses. Hazard quotient
relative water contents, photosynthetic pigments, and and hazard index indicated significant health risk due
lower total phenolics. This result shows that the to metal/metalloid exposure through consumption of
presoaking application of SA was an effective way to tainted fishes from Ganga. Target cancer risk
improve the salt tolerance of maize plants. assessment showed high carcinogenic risk from As,
Cr, Ni and Pb as well as residues of DDT and HCHs. found 28.66±0.97, 31.40±0.74 mg Kg-1 respectively in
Current data analysis showed that Ganga water quality two periodically saturated fields with two selected rice
is deteriorating day by day and at several place even in cultivars, gosai and satabdi. Whereas, in continuously
upper stretch of Ganga the water is not suitable for flooded field soil concentrations of bio-available As were
domestic uses. Although there is positive impact of ban 79.24±0.78, 77.52±0.21 mg Kg-1 in gosai and satabdi
on persistent pesticides with decreasing trend of fields respectively. Correlation has been made in terms
pesticide residues in Ganga water, the increasing trend of arsenic release from irrigated fields to plant uptake
of trace and toxic elements is alarming and the prolong due to As rich groundwater by deriving two proposed
exposure to polluted Ganga water and/or consumption seasonally justified equations. Soil microbial screening
of Ganga water fishes may cause serious illness was performed where 16S rRNA analysis of the same
including cancer. bacterial species from the two irrigation fields showed
Key words: Ganga river, Microbial diversity, Metal and different As tolerance. Fresh plant’s parts (shoot-root)
pesticides contamination. were analysed for biomass, lipid peroxidation, pigment
content, protein and superoxide dismutase (SOD)
SVII/O-17 enzyme in both conditions clearly indicating an
alleviation of arsenic contamination from rice grain with
Ultrastructure Modification of Arsenic certain sustainability and proved to be potential in
Stressed Rice Cultivars under Altered reducing considerable bio-available amount of arsenic
in dry-wet irrigation.
Irrigation Practices in Bengal Delta
Key words: Arsenic stress, Periodical water cycling, Paddy
Basin plant ultrastructure, Sustainable mitigation.
significantly decreased germination percentage, biosynthesis and its response against As(III) in plants
tolerance index, root lengths as well as fresh and dry remains to be explored. The GABA (0.5 mM)
weight as compared to control. Exogenous SNP effectively recovered the growth parameters against
attenuated the inhibition of wheat seed germination. As(III) stressed rice seedlings. Simultaneously,
The promoting effect was most pronounced at 100ìM application of GABA (0.5 and 1mM) against As(III),
SNP. The concentration of Fe and Cr was significantly consistently enhanced the unsaturated fatty acids
decreased by SNP treatment in the toxic seedlings. (USFA) (cis-10-pentadecenoic acid, oleic acid, á-
Toxicity of Fe and Cr caused oxidative stress by lenolenic acid and ã-lenolenic acid) content, which was
elevating hydrogen peroxide (H2O2), malondialdehyde higher than saturated fatty acid (SFA). Among the
(MDA) and proline contents in roots of wheat seedlings. USFAs, level of linolenic acid was found to be always
Treatment with 100 ìM SNP counteracted Fe and Cr higher with GABA application. Similarly, the elevated
toxicity by reducing the H2 O2, MDA and proline level of AAs (proline, methionine, glutamic acid and
contents of toxic Fe and Cr exposed seedlings. cysteine) was observed with the application of GABA
Meanwhile, application of SNP markedly reduced the (0.5 and 1 mM) against As(III) treatments. Among all
activities of superoxide dismutases (SOD), catalases the PAs i.e., putrescine, spermine and spermidine, level
(CAT), peroxidase (POD), ascorbate peroxidases of putrescine was found to be highest with application
(APX), glutathione reductase (GR),non protein thiols of GABA. Elevated level of USFAs, AAs and PAs
(NPT) and increased ascorbate (ASc) compared with were strongly correlated with the reduced level of
Fe and Cr toxic treatment alone, thereby indicating the hydrogen peroxide (H2O2 ) and thiobarbituric acid
modulation of the antioxidative defense system against reactive substances (TBARS) in seedlings. Overall,
Fe and Cr induced oxidative stress by NO. the study demonstrates that GABA plays a vital role in
Key words: Wheat seedlings, Fe toxicity, Cr toxicity, Sodium As(III) tolerance by enhancing the biosynthesis of
nitropruside, Oxidative damage, Antioxidative enzymes. USFA, AA and PA in rice seedlings.
Keywords: Arsenic, Amino acid, GABA, Fatty acid,
SVII/O-19 Polyamines, Oryza sativa.
content were measured by CHNS analyser and NUE variety, which resulted in poor growth, and also
was calculated. Based on the N metabolism, N indicated the adverse impact on biochemical parameters,
utilization and N content accessions were screened out Higher doses i.e. RD and 2RD reduced the Chlorophyll
as High NUE and Low NUE varieties. Other a (25-40%), Chlorophyll b (30-60%), Total Chlorophyll
biochemical parameters like total protein content, total and Carotenoids (25-50%) in comparison of control
soluble sugar content and total phenol content was also plants.
measured. Significant differences among genotypes and Key words: Mustard, Bavistin, Growth parameters,
N-treatments were found. Biochemical parameters.
Key words: NUE, cabbage, Nitrogen, NR activity, Screening,
Genotypic variability. SVII/P-3
affected after 7 and 21 days at higher dilutions i.e. including control. After 60 DAS (Days after sowing)
10%resulted to induce stress in both the test varieties. observation found that all experimental parameters
The results indicate that the sugar mill effluent may be were maximum in both Vigna crops with combined
applied for irrigation in appropriate dilutions with application of fertilizers as DAP (1.0 g) + PSB (10 g)
judicious approach to encourage industrial waste per kg of soil as compared to control and separate
management. applications of fertilizers also showed better result than
Key words: Sugar mill effluent, Raphanus sativus, Chla, Chl control. Therefore, it can be concluded that a balanced
b, Total Chlorophyll, Germination percentage, Seedling fertilizer strategy with or without combination of
vigour. chemical and biofertilizer could be used to achieve
maximum crop yield without contaminating the
SVII/P-4 environment.
Key words: Fertilizer, DAP, PSB, Vigna species,
Effect of Different Levels of Fertilizers Morphological characters.
on Morphological Characteristics of
SVII/P-5
Two Vigna Species
Percussion of Plant Derived Smoke
Supriya Dixit* and R.K. Gupta
and Karrikinolide on Germination,
Department of Botany, Dayanand Vedic College, Orai, Jalaun-
285001, INDIA, Email: supriyadixit.dixit2@gmail.com, Growth, Photosynthesis and
gupta.rk@radiffmail.com Nutritional Values of Carrot
Current soil management strategies are mainly
dependent on inorganic chemical based fertilizers, Arshiya Akeel 1*, M. Masroor A. Khan1, Hassan
which caused a serious threat to human health and Jaleel1 and Moin Uddin2
environment. Today fertilizer has become essential to 1
Department of Botany, Aligarh Muslim University, Aligarh,
modern agriculture to increase yield and to feed the INDIA; 2Botany Section, Women’s College, Aligarh Muslim
growing population. Increased crop production largely University, Aligarh, INDIA, Email: arsh.aqeel@gmail.com,
relies on the type of fertilizers used to supplement masruur@gmail.com, jaleelamu@gmail.com, moinuddin.bt
essential nutrients for plants. Microorganisms play an @gmail.com
important role in soil processes that determine the plant Plant-derived smoke has now been established
productivity. Today most of farmers and agriculturists as a positive regulator of seed germination and growth
are turning to the use of biofertilizers, to enhance the in many plant species. Out of several compounds
crop production, as these are gentler on the soil as present in plant-derived smoke, the major active
compared with the chemical fertilizers. A pot growth-promoting compound is believed to be
experiment carried out during two kharif seasons to Karrikinolide or KAR1 (3-methyl-2H-furo [2,3-c] pyran-
study the effect of different levels of chemical fertilizer 2-one). A pot experiment was performed under the
(DAP) (0.25g, 0.5g and 1.0g per kg of soil) and natural conditions of net house at the Department of
biofertilizer (PSB) (2.5g, 5.0g and 10g per kg of soil) Botany, Aligarh Muslim University, Aligarh, India.
separately and in combination on urd bean [Vigna Smoke-water making apparatus was designed in the
mungo (L.) Hepper] and mung bean [Vigna radiata lab. This study was conducted to test the efficacy of
(L.) Wilczek] to observe certain morphological smoke-water and KAR 1 on carrot (Daucus carota
characteristics i.e. germination percentage, plant height, L.). Smoke-water and KAR1 treatment was applied to
fresh and dry weights of plants, leaf area and total the plants in the form of diluted aqueous solution of
chlorophyll content. The study was aimed to improve variable concentrations. Seeds were pre-soaked in the
the growth and obtain optimum yield of Vigna species solutions of smoke-water and KAR1. Analyzed aspects
with eco-friendly, non-toxic way and reduce the use of of this study were: percent seed germination, plant
chemical fertilizer application in agricultural activities. growth, photosynthesis and nutritional values. The
In the present study, sixteen treatments were prepared results obtained clearly indicate a significant
improvement in all the attributes studied. In case of During the nuclear division, the damage of genetic
seed germination percentage, SSW 1:500 (v/v) and 10- material was observed from lower to higher
8
M of KAR 1 showed an increase of 58.06% and concentrations. Genetic damage index (GDI) during
54.39% respectively, as compared to control. The â- nuclear division has shown a mixed response in A.
carotene content and ascorbic acid content exhibited chinense (19.7, 14.26, 21.07) and A. tuberosum (12.24,
an increment of 32.71% and 37.90% over control by 39.54, 18.36) as the GDI material increase from lower
1:500 (v/v) of SSW and KAR 1 10 -8 M showed to higher concentration but the apoptosis in the cells
enrichment of 42.03% and 48.42% respectively in showed above 90% in all the Allium species. When
comparison to control. This study provides insight into the meiotic index decreased at higher concentration, it
an affordable and feasible method of crop improvement. also indicates that the total percent of abnormal cell
Key words: 3-methyl-2H-furo [2,3-c] pyran-2-one, Daucus must increases at higher concentration. The frequency
carota L., Growth promoter, Karrikinolide, Plant-derived of getting abnormal cells increased from lower to higher
smoke, Photosynthesis, Seed germination. concentration in A. hookeri (2.54, 1.66, 11.75) and A.
porrum (1.90, 3.33, 6.07), which may be also
SVII/P-6 suggested that A. hookeri and A. porrum are more
sensitive to the applied concentration. Since the
Effect of Sodium Dodecyl Sulphate frequency of abnormal cell increased after application
(SDS) to Determine the Indices of of SDS, A. hookeri and A. porrum may be considered
more sensitive than A. ascalonicum (3.26, 5.06, 4.43),
Allium Species Studied A. tuberosum (4.83, 9.04, 3.95) and A. chinense
(16.38, 3.44, 4.34). When the concentration was applied
Thejasenuo Julia Kirha 1 *, Sanjay Kumar 2 and to the Allium species for SDS salt tolerance the higher
Talijungla1 concentration has shown less tolerance in A. porrum
1
Department of Botany, Nagaland University, HQs: Lumami- (0.25, 0.24, 0.14) and A. hookeri (0.38, 0.43, 0.17).
798627, INDIA; 2Department of Botany, Banaras Hindu Which suggests that it involves higher number of
University, Varanasi-221005, INDIA, Email: thejasenuokirha abnormal cells at higher concentration, and it also
@gmail.com, ksanjay79@gmail.com, lkr.talijungla@ correlates with the higher production of abnormal cells
gmail.com frequency, GDI, apoptotic index and meiotic index.
Sodium dodecyl sulfate (SDS) is a primary alkyl None of the species has shown 50% of the salt
sulphate denoted by molecular formula C12H25NaO4S tolerance which also suggested that all the species are
with molecular weight of 288.38 g mol ”1 .T he sensitive to the applied concentration but most sensitive
tremendous use of this chemical in daily life activity species is A. hookeri and A. porrum.
prompted us to determine the Indices of this chemical Key words: SDS, Allium species, Abnormalities, Genotoxicity,
in plant genetic system. The different Allium species Toxicity.
(A. hookeri, A. tuberosum, A. chinense, A.
ascalonicum, A. porrum) and concentrations (500, SVII/P-7
1000 and 3000 ppm) was used in the present study to
observe the said effects for 3 h on the genetics of this Impact of Silicon: Most Neglected
species. Different Indices such as meiotic index (MI), Nutrient, in improving Quality and
apoptotic index (AI), nuclear index (NI) and genetic
damage index (GDI) were calculated along with Quantity of Menthol Production in
frequency of chromosomal aberrations, total no. of Mentha arvensis
abnormal cells, SDS tolerance were taken into
consideration after the application SDS in different Vinod Goyal1*, Sanjay Dwivedi 2 , Anita Kumari 1 ,
concentration in all the Allium species. The nuclear Neeru Jain3 and R.D. Tripathi2
division index which indicates the division rate of a 1
Department of Botany & Plant Physiology, CCS HAU, Hisar-
nucleus shows 100 percent division of nucleus under 125001, INDIA; 2Plant Ecology and Environment Science
the meiosis after application of different concentration. Division, CSIR-NBRI, Lucknow-226001, INDIA; 3Privi Life
SVII/P-9 SVII/P-10
determine the presence of specific polyphenols in all these parameters could be correlated to their differential
the genotypes and gallic acid was the detected As uptake with sensitive varieties displaying higher As
phytochemical. Overall results suggest that guar seeds contents. Increase in the levels of total soluble sugar
can be a good source of natural antioxidants for the and proline proved to be important stress markers
development of nutraceuticals and functional foods. responsible for imparting better As tolerance in HC3
Key words: Cyamopsis tetragonoloba, Genotypes, and HC5 than other genotypes.
Antioxidant, Free radical-scavenging activity, Reducing Key words: Arsenic, Chickpea, Osmolytes, Nodulation
power. potential.
SVII/P-11 SVII/P-12
glutaredoxin (SlGRX1) was found to be involved in an important role played by urban dust in determining
drought stress. During our investigation, we have found the levels of chlorophyll pigments in the foliar surfaces
that chickpea GRX (LOC101493651) was upregulated of indoor plants. By the end of this study, we will be
many folds under drought stress. We are presently able to characterise the tolerant species among the
exploring the role of different GRXs under drought selected plants in polluted indoor environment. Such
stress, using molecular approaches. knowledge is beneficial for mitigating indoor air pollution
Key words: Glutaredoxin, Drought, ROS, Abiotic stress. in urban cities.
Key words: Dustfall flux, Chlorophyll pigments, Urban cities,
SVII/P-13 Indoor plants, Foliar surface.
Delhi is one of the most polluted cities having Regions of the Earth that experience abundant
poor air quality which has an adverse impact on the sunshine and warm temperatures are conducive to plant
health of the individuals. People staying indoors for growth and crop productivity. They are also susceptible
longer hours have to face various air pollution problems. to generation of oxidant air pollutants in the form of
Among living beings, plants are the bystanders facing both gases and particles. When natural ventilation is
pollution. They act as a natural sink for airborne poor, as in basins, valleys, and areas of low wind,
pollutants. The foliar surfaces of plants act as biological unpleasant or dangerous levels of these pollutants may
filters to detoxify harmful gases and also serve as a accumulate. Many of the world’s mega-cities fall into
platform to trap dust. But the deposition of dust onto this category, as does the San Joaquin Valley of
the foliar surfaces affects the physiological and California. In some areas these are also wintertime
biochemical properties of the plants depending upon problems. Drought stress is a concern of agricultural
the tolerance and sensitivity of the species. The level and wild land plant biologists as global change occurs,
of sensitivity varies among different plants depends on including “global change type drought”. This reflects
their response towards characteristic environmental the increase in evaporative demand due to elevated
stress. The present study includes three indoor plants temperature, even if precipitation is stable. Ozone, the
namely, Ficus elastica, Ficus lyrata and Schefflera, most damaging gaseous air pollutant to vegetation,
growing at two urban localities of distinct characters reduces carbon allocation to roots, root proliferation,
in Delhi during summer season. We aim to find out the and water harvesting. Ozone also reduces steady state
dust fall fluxes on the foliar surfaces and its effect on stomatal conductance and induces changes in the rate
the chlorophyll content of these plants. The average at which stomata respond to environmental variables.
dust fall flux for Ficus elastica, Ficus lyrata and When slow closure leads to longer open periods in high
Schefflera is 199, 275 and 191 mgm-2d-1 respectively light or dry air, water loss increases and drought
at Mayapuri and 178, 192 and 130 mg m -2 d -1 hardiness declines. Particulate matter is less well-
respectively, at Dwarka. It has been seen that higher studied. Many aerosol species are hygroscopic, which
dust fall flux at Mayapuri shows a clear reduction in increases dew deposition on leaves, increases
the chlorophyll pigments of all the three plants at deposition of gases such as ozone and ammonia, and
Mayapuri, as compared to Dwarka. This trend indicates penetrates the stomatal pore creating a thin liquid phase
pathway between leaf apoplast and the atmosphere. nilotica. Further, we investigated the allelopathic
This reduces drought hardiness. These impacts of air potential and soil properties of Prosopis juliflora in
pollutants are just beginning to be incorporated into terms of pH, conductivity, phenolics and organic matter
models of regional hydrology. The accuracy of such in control soil and Prosopis soil. Prominent alteration
modeling efforts is poor, due to the very preliminary was observed in various above-mentioned parameters
understanding of mechanisms. More research on in invaded soil.The present study clearly inflicts the
vegetative impacts of gases and particles is urgently invasive nature of Prosopis juliflora as it possesses
required. better functional traits and allelopathic potential which
Key words: Ozone, Particulate matter, Plant productivity, Plant further inhibits the growth and germination of local
water relations, Stomata. species.
Key words: Plant functional traits, Leaf traits, Stem traits,
SVII/P-15 Root traits, Allelopathy.
starvation response 1) and putative SNRK2-type objective to propagate and enhance the biomass of this
kinases, as these proteins have been previously potential moss ‘in vitro’. Plants of Rhodobryum roseum
implicated in metabolic control in algae and other were cultured in two different combinations of culture
organisms. Genetically modified lines were used to media: Knop’s + 2,4-D and Knop’s + vitamin B1 using
evaluate the role of these proteins in storage metabolite stem as explant. In this study role of exogenous
biosynthesis in response to nutrient stress. C. application of a plant growth regulator called 2,4-D (a
reinhardtii were grown under nutrient limiting and synthetic Auxin) and vitamin B1 (as a cofactor) has
sufficient conditions and analysed using combinations been observed. Study has shown that Rose moss grown
of high-throughput metabolic profiling by Fourier in culture media exogenously supplemented with 2,4-
transform infrared (FTIR) spectroscopy, and detailed D have high protonemata density, while plants grown
metabolite analysis by chromatography–mass in medium with Vitamin B1, produced longer stem with
spectrometry (LC- and GC-MS), whole transcriptome wider and robust leaves as compared to plants grown
analysis by RNA sequencing and validation by simply in Knop’s medium. Subsequently use of
quantitative real-time PCR. The analysis showed combination of 2,4-D and Vitamin B1 in Knop’s medium
specific metabolic and transcriptomic fingerprints in has resulted into enhanced plant and leaf size with
response to specific nutrient limitation conditions that density of protonemata. Hence it is a better strategy to
correlate with lipid and starch biosynthesis. enhance the biomass of this medicinally important moss
Furthermore, PSR1 was shown to function as a for bioprospection.
regulator of carbon metabolism specifically under P Key words: Rhodobryum roseum, Vitamin B1, 2,4-D, Biomass.
limiting conditions, by controlling the expression of
specific metabolite biosynthesis enzymes. SVII/P-20
Key words: Microalgae, Chlamydomonas reinhardtii, Lipid,
Starch. Role of Exogenous Polyamines on
Plant Biomass, Nutrient Status,
SVII/P-19
Osmolytes Synthesis and Yield
In vitro Propagation of Rhodobryum Parameters in Cajanus cajan (L.)
roseum Limpr. with Exogenous Millsp. (Pigeon pea) Genotypes under
Application of 2, 4-D and Vitamin B1: Nickel Stress
A Strategy to Enhance Biomass
Kiran Saroy* and Neera Garg
Isha Pathak* and A.K. Asthana Department of Botany, Panjab University, Chandigarh-
Bryology Laboratory, CSIR-National Botanical Research 160014, INDIA, Email: kiransaroy747@gmail.com, gargneera
Institute, Lucknow-226001, INDIA, Email: ishapathak69 @gmail.com, garg_neera@yahoo.com
@gmail.com Nickel (Ni) is an essential micronutrient for plants,
Present study highlights in vitro propagation of but its excessive concentration in the soil is considered
Rhodobryum roseum known as “Rose Moss” toxic for plant growth and development. Polyamines
belonging to family Bryaceae. It is a cosmopolitan (PAs) such as putrescine (Put), spermidine (Spd) and
taxon growing at higher altitudes in cool and shady spermine (Spm) are small aliphatic nitrogenous
places. Reproduction of this species commonly takes compounds and have been reported to play significant
place vegetatively through horizontal stems or stolons. roles in the regulation of heavy metal (HM)induced
This species is medicinally important to treat nervous toxic responses in plants. Experiments were conducted
prostration and cardio-vascular diseases. In spite of its to investigate the relative roles of Put, SpdorSpm
potential medicinal value it cannot be fully utilized for (0.5mM) on plant biomass, nutrient status, osmolytes
isolation of chemical compounds due to little biomass synthesis and yield in two pigeonpea genotypes
in nature. Present work has been carried out with an (Tolerant - Pusa 2001, Sensitive - AL 201) subjected
to nickel stress (NiSO4 - 100 mg kg-1). Ni stress had is a transcriptional activator which contains a DNA
more negatively effects on roots as compared to shoots binding domain which is a highly conserved, a TIG
which led to a decline in root to shoot ratio. A significant domain which is an immunoglobulin chain like domain,
decrease in nutrient (N, P and K) concentrations an ANK repeat which helps in protein-protein
resulted in reduced productivity which had a direct interaction and an IQ motif. In Arabidopsis, studies
correlation with increased Ni uptake in both the with the CAMTA gene have showed steady recovery
genotypes. Pusa 2001 had significantly greater potential after drought and also under different abiotic stresses.
to withstand Ni stress when compared with AL 201. Therefore, CAMTA can play a major role during
Seed priming with polyamines reduced Ni uptake, drought conditions and could open new perspective for
improved plant growth, nutrient acquisition and the improvement of crops for enhancement of stress
productivity in a genotype dependent manner with tolerance and thereby lead to enhanced crop
higher positive effects displayed by Put followed by productivity.
Spd and Spm. Although, increase in osmolytes status Key words: CAMTA, WUE, RWC, Drought recovery.
was recorded under Ni stress PAs treatments further
boosted the osmolyte synthesis especially proline. The SVII/P-22
study concluded that Put was more effective than Spd
and Spmin imparting tolerance to Ni stress in Pigeonpea Ethylene Di Urea as Ozone
plants.
Protectant under High Tropospheric
Key words: Polyamines, Nickel, Pigeonpea, Nutrient,
Osmolyte.
Ozone in Two Mung Cultivars
activities of antioxidant glutathione and antioxidative trend with excess Zn levels. Therefore, the above study
enzymes in both the cultivars as compared to non EDU concluded that the elevated levels of Zn in soil, showed
treated plants. However, insignificant increase was inhibitory effects on growth and biochemical
recorded in physiological parameters viz. stomatal constituents as studied in Trigonella plants while the
conductance, photosynthesis, and water use efficiency lower levels of Zinc (2 and 4 ppm)promoted its growth.
in EDU treated plants. Significant increments in yield Key words: Zinc, Trigonella, Antioxidative enzymes, Protein,
parameters like no. of pod plant-1, pod weight plant-1, Chlorophyll.
no. of seeds plant-1, and hundred seed test weight were
observed in both the cultivars under EDU treatments. SVII/P-24
Results revealed that EDU concentration of 300 ppm
was more effective than 200 ppm in alleviating the Screening of Cajanus cajan (L.)
negative effects of ozone toxicity in SML-668 cultivar. Millsp. (Pigeonpea) Genotypes for
SML-668 was found to be more sensitive (EDU
responsive) than PDM-139 cultivar. The present study their Arsenic Tolerance in Terms of
demonstrated that EDU can be used as valuable Germination, Seedling Growth, ROS
research tool to screen sensitive/tolerant cultivars under
high ambient ozone.
Generation and Osmotic Balance
Key words: Tropospheric Ozone, EDU, Mung, Yield, Vigna Shyna Bhalla* and Neera Garg
radiata.
Department of Botany, Panjab University, Chandigarh-
160014, INDIA, Email: shynabhalla@gmail.com, gargneera@
SVII/P-23
gmail.com; garg_neera@yahoo.com
Growth, Pigments and Antioxidative Presence of heavy metals (HMs) in the soils has
Responses of Trigonella foenumg- become a potent threat to agriculture due to their non-
degradable nature. Arsenic (As) is a non-essential toxic
raecum against Excess Zinc in Soil metalloid which gets accumulated in plants and its
elevated concentration adversely affects germination
Sanjoli Tripathi* and S.N. Pandey and plant growth. The study was aimed to investigate
Department of Botany, University of Lucknow, Lucknow- the comparative effects of different concentrations of
226007, INDIA, Email: sanjolitripathi@yahoo.com inorganic forms of As, i.e. AsV (Arsenate-0, 10, 20,
Clay pot culture experiment was conducted in 30, 40, 50 mg kg-1) and AsIII (Arsenite- 0, 2, 4, 6, 8, 10
glass-top wire house to study the excess levels of zinc mg kg-1 ), on seed germination, plant biomass, ROS
amendment viz.nil (control), 2, 4, 10 and 20 ppm Zn production and osmolyte synthesis in four genotypes
(as ZnSO 4 ) in soil on growth and antioxidative (PUSA2001, PUSA2002, AL882 and MANAK) of
responses of Trigonella foenumgraecum. The growth pigeonpea. 50 mg kg-1 of AsV and 10 mg kg-1 of AsIII
(length, dry weight yield and branching) and biochemical were found to be critical concentrations that significantly
properties (total chlorophyll, protein content and the delayed and reduced the seed germination, seedling
activity of catalase and peroxidase) were measured in growth, vigor index, emergence percentage, respiration
Trigonella foenumgraecum leaves at 60 days of rate etc. in all the four genotypes. On the basis of metal
growth. The length and dry weight of plants increased tolerance index (MTI), PUSA2001 was found to be
up to 4 ppm zinc levels in soil, Thereafter decreased at more tolerant, PUSA2002 moderately tolerant,
its higher concentration. Total chlorophyll contents also MANAK and AL882 highly sensitive to As. A
followed similar trends. Protein content in leaves significant decline in root and shoot length as well as
increased about two times more at elevated Zn levels their dry weights were recorded in concentration and
in soil as compared to native soil without amendment. genotype dependent manner with more negative impact
The activity of catalase in plants was not affected under the combined treatments of AsV and AsIII.
significantly with gradual increase of the Zn levels in Decline in plant biomass had direct effect on leaf water
soil. Whereas peroxidase activity showed decreasing status and photosynthetic pigment concentration. AsIII
was more toxic and induced higher oxidative stress in which was declined in roots. The polyphenols content
terms of increased hydrogen peroxide and was also altered in all the treated concentrations as
Malondialdehyde contents. There was a significant compared to control. Furthermore, the exposure of BBP
increase inproline accumulation in the leaves which induced stomatal abnormalities, cytotoxicity, and loss
played the role of osmoprotectant and imparted of plasma membrane integrity in the treated plants.
relatively higher As tolerance in PUSA2001. Presently, BBP disturbed the normal physiology of
Key words: Arsenic, Pigeonpea, Photosynthetic pigments, barley which could also affect the yield of the crop
ROS, Osmolytes. under field conditions.
Key words: Benzyl-butyl phthalate, Hordeum vulgare L.,
SVII/P-25 Oxidative stress, Proline, Polyphenols, Cytotoxicity.
SVII/P-27 SVII/P-28
physiological changes in plants. The results showed the curve in variety RGC-936 decreased around 22%
that the virus with heavy metals retards the growth of in RGC-936 then 10% in RGC-1066 under water deficit
shoots as well as roots. Interestingly high conc. of metal condition. The decrement in the Jmax values signifies
inhibits the accumulation of virus. This study suggested shift in the maximum electron transport rate process
that using non-toxic concentration of metals may protect caused by water stress. The unutilized CO2 in plants
plants from viral infection. at saturating CO2 concentration i.e, triose phosphate
Key words: Arsenic, Begamovirus, Defense related enzymes, utilization increased significantly in RGC-1066 than in
Nicotiana tabacum. RGC-936. At saturating CO2 there is non stomatal
limitation of CO2 assimilation because of reduction in
SVII/P-31 ATP synthesis and RuBP regeneration. The rate of
CO 2 assimilation under drought in different
Alterations in CO2 Assimilation Vs photosynthesis limiting conditions is efficient in RGC-
Internal CO2 Response Curve under 1066 than RGC-936 showing intraspecies variation and
performance of the two varieties at physiological level.
Water Deficit Condition in Cyamopsis
Key words: Internal CO2, CO2 assimilation, Vcmax, Jmax, TPU,
tetragonoloba (L.) Taub Intraspecies variation.
diphosphate synthase, cytochrome P450, squalene complexation of arsenite with thiol-rich peptides. Thus,
synthase, sterol Ä-7 reductase, sterol glycosyl maintaining sufficient Sulfur nutrition may be
transferase and sterol desaturase) were upregulated particularly important in As-contaminated
in treated and microbial consortium plants under environments. Arsenic contamination is a major problem
A.alternata stress. In addition, use of endophytes not in rice growing areas hence sulfur nano particles may
only increase the content of withanolide A, withaferin be supplemented with the nutrients given to the plants.
A and withanolide B (3.32-5.96, 1.52-1.96 and 12.49- Due to its small size and shape, it may easily enter and
21.47 fold respectively) under A. alternata infection translocate in to the plants and thus reduce arsenic
but also provide the resistance to host via improvement toxicity.
of their photochemical efficiency, standardizing the non- Key words: Arsenic, Sulfur nano particles, Toxicity, Rice.
oxized and oxidized fractions, accelerating electron
transport rate and photochemical and non- SVII/P-34
photochemical quantum yield. Furthermore, depletion
in passively dissipated energy of PSI and PSII in Evaluation of Leaf Proteomics
microbial combination treated plants validates the above
findings. Altogether, the study provides novel insights
Responses during Selenium Mediated
into the application of endophytes and their ability to Tolerance of Arsenic Toxicity in Rice
accelerate withanolide biosynthesis in W. somnifera (Oryza sativa L.)
under biotic stress caused by A.alternata.
Key words: Endophytes, Bacillus amyloliquefaciens, Reshu Chauhan 1,2 *, Surabhi Awasthi 1 , Sanjay
Pseudomonas fluorescens, Withania somnifera, Dwivedi1, Lalit Agrawal1, Shekhar Mallick1, Veena
Withanolides. Pande2 and R.D. Tripathi1
1
CSIR-National Botanical Research Institute, Rana Pratap
SVII/P-33
Marg, Lucknow-226001, INDIA; 2Department of
Biotechnology, Kumaun University, Bhimtal, Nainital,
Sulfur Nanoparticle Mediated Uttarakhand-263001, INDIA, Email: reshu.biotech.87@
Detoxification of Arsenic Stress in gmail.com
Rice (Oryza sativa L.) Arsenic (As) is a toxic element, which poses
significant threat to human health due to frequency of
Garima Awasthi1,2*, Amit Kumar2,3, Kiran Gupta2 , occurrence, toxicity and various routes for human
Kumkum Mishra2 and R.D. Tripathi3 exposure. Arsenic contamination of water and food,
1
Vivekananda Global Universit, Jaipur-302012, Rajasthan,
especially rice, is a serious issue. Selenium (Se) is an
INDIA; 2University of Lucknow, Lucknow-226007, India; essential element for human and other animals and is
3
CSIR-National Botanical Research Institute, Lucknow- beneficial for plants as well. Selenium has been found
226001, INDIA, Email: gariimaa21@gmail.com to antagonize the toxicity of As in plants. Importantly,
rice is a good accumulator of both As and Se and hence,
Arsenic (As), a ubiquitous toxic metalloid element
the application of Se to rice can be a feasible strategy
in earth crust and has become a global concern in many
to mitigate As toxicity. The present study focused on
regions of the world due to geochemical weathering of
understanding proteomic responses of leaves of arsenite
rocks, microbial and human activities. Arsenic is an
(AsIII) stressed rice plants under the supplementation
environmental and food chain contaminant. Indian soils
of selenite (SeIV). Supplementation of SeIV (25µM)
are sulfur deficient making crop sulfur deficient. Sulfur
significantly decreased As accumulation in rice plants
is a ubiquitous and essential element for all living
as compared to that of AsIII (25µM) alone treated
organisms, like its counterpart’s carbon and nitrogen,
plants. The proteomic study was also performed using
plays a pivotal role in plant metabolism and
2-dimensional gel electrophoresis (2DE). By
development. Sulfur deficiency has considerable impact
comparison of gel images of different treatments viz.,
on plant growth and development, reducing crop yield
control, As and Se with the help of PDQuest software,
and quality. Sulfur helps to detoxify As through
89 differentially expressed proteins were identified in subjected to arsenate [As(V)] stress alone and also
rice leaves using MALDI-TOF/TOF. Functional with microbial consortium for 15 days. The presence
categorization revealed that As and Se responsive of consortium promoted the growth of the plants and
differentially expressed proteins are mostly related to accumulation of nutrients by reducing the toxicity of
defense, photosynthesis, redox homeostasis, antioxidant As. Two-dimensional gel electrophoresis (2DE) analysis
system, amino acid metabolism, energy metabolism and revealed that 145 spots were significantly expressed,
transport. Protein ubiquitinisation and phosphorylation out of these, 79 spots were identified using MALDI-
also might be playing important roles in Se mediated TOF-TOF. The differentially expressed proteins during
amelioration of As toxicity in rice. In conclusion, the supplementation of microbial consortium under As stress
present study provides new insights into As-Se belonged to defense, growth promotion, energy
interaction in rice leaves at the proteome level and metabolism, cellular and metabolic processes. This
identifies crucial proteins involved in Se mediated study revealed the expression of IAP100 (Inhibitor of
amelioration of As toxicity in rice. Apoptosis) in the presence of microbial consortium
Key words: Arsenic, Selenium, Rice, Protein profile. under As stress, which has ability to regulate caspases
and also influence ubiquitin (Ub)-dependent pathways
SVII/P-35 that modulate innate immune signaling in plants. In
conclusion, these results provide new insights about
Evaluation of Proteomics Responses the proteins and mechanisms involved in microbial
consortium mediated As toxicity amelioration in rice.
of Rice (Oryza sativa) during Arsenic
Key words: Arsenic, Pseudomonas putida, Chlorella
Toxicity Amelioration by a Potential vulgaris, Rice.
Microbial Consortium
SVII/P-36
1,2* 1
Surabhi Awasthi , Reshu Chauhan , Sudhakar
Srivastava1 , Sanjay Dwivedi1, Lalit Agrawal1 and Selenium Mitigates Arsenic Toxicity by
R.D. Tripathi1 Modulation of Phenolics, Antioxidant
1
CSIR-National Botanical Research Institute, Rana Pratap System and Thiols in Rice (Oryza
Marg, Lucknow-226001, INDIA; 2Institute of Environment
& Sustainable Development, Banaras Hindu University, sativa L.)
Varanasi-221005, INDIA, Email: surabhi.microbiology@
gmail.com Reshu Chauhan1*, Surabhi Awasthi 1 , Sudhakar
Srivastava2, Sanjay Dwivedi1 and R.D. Tripathi1
Environmental pollution of arsenic (As) is a
1
serious problem that is not only affecting the local CSIR-National Botanical Research Institute, Rana Pratap
people but also population throughout the world through Marg, Lucknow-226001, INDIA; 2Institute of Environment
rice and rice based food products. The scale of problem & Sustainable Development, Banaras Hindu University,
Varanasi-221005, INDIA, Email:reshu.biotech.87@gmail.com
is reaching high with the discovery of new As
contaminated regions. Rice accumulates As in Arsenic (As) contamination of paddy rice is a
significantly greater amounts in comparison to other serious threat all over the world particularly in South
cereal crops. Microbiological interventions can serve East Asia. Narrow range of selenium (Se) is beneficial
as protective agents to ameliorate As stress in rice. to plants as well as arsenite (AsIII) and selenite (SeIV)
The present study was carried out to explore can be biologically antagonistic to each other due to
mechanistic details of a microbial consortium [plant having same electronic structure and sharing the same
growth promoting rhizobacterium, Pseudomonas transporter for their uptake in rice. Thus during the
putida and alga, Chlorella vulgaris] mediated As present study, the detoxification mechanism of As
toxicity amelioration in rice (Oryza sativa) using toxicity was explored by evaluating As accumulation,
comparative proteomic approach. The experiments growth, oxidative stress, phenolic compounds,
were conducted in hydroponically grown rice seedlings antioxidant enzymes activity and thiols during AsIII and
SeIV interaction in hydroponically grown rice seedlings. are posing serious threat to biota including plants due
The results indicated that the presence of Se (25µM) to their release into environment. This may affect
significantly decreased As accumulation in roots and adversely the production of staple crops. Rice (Oryza
shoots as compared to As (25µM) alone treated plants. sativa) is a global staple food for a large population.
Oxidative stress was analyzed by detection of reactive CuO NPs (size <50 nm) inhibit the root and shoot growth
oxygen species (ROS), lipid peroxidation, electrical of rice seedlings in concentration dependent manner in
conductivity (EC), nitric oxide (NO) and pro-oxidant comparison to control. Upward translocation of CuO
enzyme (NADPH oxidase), which were in the order NPs result in accumulation of NPs in cell wall,
of As>As+Se>control>Se for both roots and shoots. chloroplasts etc. Rice plants under NPs (CuO) stress
Selenium combat As phytotoxicity by increased level combat toxicity generated by reactive oxygen species
of phenolic compounds particularly gallic acid, (ROS) through hyperactivity of antioxidant defense
protocatechuic acid, ferulic acid andrutin, antioxidant system comprised of antioxidant enzymes and cellular
enzymes, non-protein thiols (NPTs) and thiol metabolism antioxidants. However, roots are the first plant part
related enzymes viz., serine acetyl transferase (SAT), exposed to stress generated by CuO. Hence, they have
cysteine synthase (CS) and glutathione-S-transferase shown toxic symptoms viz. browning of the roots and
(GST). Selenium supplementation also enhanced the lower hyper response of the antioxidant defense
uptake of nutrient elements viz., Fe, Mn, Co, Cu, Zn, system. CuO NPs induce drought like condition due to
Mo, resulted in improved plant growth. The results adhesion of large agglomerates on roots. One of the
concluded that Se addition in As contaminated known marker for water stress, proline, increased
environment might be an important strategy to reduce dramatically in all treated samples to provide an
As uptake and associated phytotoxicity by modulation osmoprotective function when compared to their
of thiols and phenolic compounds and increased uptake respective controls. Transcripts of PIN-1 gene was
of nutrient elements in rice plant. down regulated in leaves and roots of rice. Further,
Keywords: Arsenic, Nutrient elements, Phenolics, ROS expression of PIN-2 and AUX1 genes were upregulated
staining, Selenium in these plants. Auxin distribution in roots mediated by
auxin efflux carriers was probably responsible for root
SVII/P-37 growth inhibition in rice exposed to CuO NPs. Thus,
rice plants attempt to reduce Phytotoxicity caused by
Phytotoxicity and Metabolic NPs by metabolic adaptations.
Adaptations in Rice Exposed to Keywords: Copper oxide nanoparticles, Copper ions,
Phytotoxicity, Metabolic adaptations.
Copper Oxide Nanoparticles and
Copper Ions SVII/P-38
Archana Dwivedi1*, Subodh Kumar1, Alok Kumar CuO Nanoparicles Affects Growth,
Pandey2 and Poornima Vajpayee1 Photosynthetic Pigments and Cellular
1
Ecotoxicology, Bioremediation and Nanotoxicology Antioxidants in Ocimum basilicum
Laboratory, Department of Botany University of Lucknow,
Lucknow-226007, INDIA; 2Nanomaterial Toxicology, CSIR-
Subodh Kumar1*, Archana Dwivedi1, Alok Kumar
Indian Institute of Toxicology Research, Vishvigyan Bhavan,
31, Mahatma Gandhi Marg, P.O. Box No. 80, Lucknow-226001, Pandey2 and Poornima Vajpayee1
INDIA, Email: devpoornima@rediffmail.com, poornima 1
Ecotoxicology, Bioremediation and Nanotoxicology
vajpayee@gmail.com Laboratory, Department of Botany University of Lucknow,
Lucknow-226007, U.P., INDIA; 2Nanomaterials Toxicology,
The increasing use of engineered metallic
CSIR-Indian Institute of Toxicology Research, Vishvigyan
nanoparticles in industry and therapeutics has led to Bhawan, 31, Mahatma Gandhi Marg, P.O. Box 80, Lucknow
growing need for investigating their potential negative 226001, U.P., INDIA, Email: devpoornima@rediffmail.com,
impact on human and environmental health. Metallic poornimavajpayee@gmail.com
nanoparticles, extensively used in commercial products
CuO nanoparticles (CuO-NPs) are globally used be categorized into textile and fabric, carpet, Diesel
in many agricultural applications such as pesticides, Locomotive Works (DLW), wood, leather, metal, paper
herbicides, fertilizers, additives for soil remediation, and products, food processing, plastic–rubber and glass
growth regulators. Copper is an essential element and industries. The Industrial effluents from these industries
is involved in several vital metabolic activities in plants. carrying heavy metal directly or indirectly (get mixed
Indiscriminate use of copper nanoparticles in various with the city sewage system) gets discharged into the
applications paves gateway for these materials into the river. These effluents contain high concentration of
atmosphere, soil, and water. Thus, the widespread use heavy metals (HMs) ultimately polluting water,
of CuO-NPs raised concerns worldwide about the sediment and soil. Metals discharged into the river get
impacts that these materials might have on staple and precipitated and accumulated onto river sediments and
medicinal crops. In this study plants of Ocimum eventually enter the food chain. Higher concentrations
basilicum were exposed to CuO (0.0-.10 µgml-1) for of heavy metals can form harmful complex compounds,
24 days in hydroponics and their impact on growth, which critically effect different biological functions.
photosynthetic pigments and cellular antioxidants was Heavy metals are not easily degraded by the natural
determined. Result indicates that CuO-NPs exhibit processes and persist in soil and sediment. This study
adverse impact on growth and photosynthetic pigments. emphasis on the effect of heavy metal toxicity on the
Root and shoot biomass was decreased in CuO-NPs benthic microbiota community, as benthic microbial
exposed plants. Chlorosis in leaves and browning of communities play crucial roles in ecological processes
the roots was also observed in plants exposed to CuO- such as organic matter recycling and biomass
NPs. An increase in MDA contents and membrane production. Due to their functional role in aquatic
permeability of CuO-NPs treated plants showed that ecosystems, it poses an ecological risk as it may impact
these particles generate oxidative stress in O. ecosystem functioning.
basilicum. Hence, it can be inferred from the present Key words: River Ganga, Heavy metal, Effluents, Toxicity,
study that CuO-NPs induced toxic effects in O. Benthic microbiota.
Basilicum could alter their medicinal properties.
Keywords: Copper oxide nanoparticles, Ocimum basilicum, SVII/P-40
Photosynthetic pigments, Phytotoxicity.
Temporal Development of Stomatal
SVII/P-39 Traits Regulate Physiological
Impact of Heavy Metal on the Benthic Responses in Cotton
Microbial Community Raghvendra Dubey 1 , Shivanand Suresh
Dudhagi2and Pramod Arvind Shirke2 *
Chitra Pokharia* and Kavita Shah
1
Plant Physiology Laboratory and Academy of Scientific and
Environmental Biotechnology Laboratory, Institute of Innovative Research (AcSIR),CSIR-National Botanical
Environment and Sustainable Development, Banaras Hindu Research Institute, Lucknow-226001, INDIA;2CSIR-National
University, Varanasi-221005, Uttar Pradesh, INDIA, Email: Botanical Research Institute, Rana Pratap Marg, Lucknow-
chitra.pokharia04@bhu.ac.in, kavitashah@bhu.ac.in 226001, INDIA, Email: raghvendra.jnu@gmail.com,
River Ganga, considered as one of the most pashirke@nbri.res.in
sacred river in the world, is one of the largest and The balance of gaseous flow between plants and
longest rivers in India. The Ganges basin is the most their atmosphere largely depend on stomatal traits
heavily populated river basin in the world, with over (stomatal density and size). Hence, in response to
400 million people and a population density of about fluctuating environmental and internal stimuli, plants
1,000 inhabitants per square mile (390 km-2). Varanasi, have to coordinate two type of regulation, a short term
situated on the bank of river Ganga, is an important response regulation by opening of stomata and long
industrial and religious centre in northern India. Varanasi term response through control of stomatal development
region has about 5000 registered industries which can that actively adjusts stomatal conductance,
photosynthesis and water loss. The mechanism metalloid [AsIII & AsV (15 to 240 µM)], salinity [NaCl
controlling stomatal development and their physiological (50 to 200 mM)] and drought stress [polyethylene glycol
significance are still not well explained. Here, we (PEG) (10 to 40 mM). Among all ten cultivars of rice
demonstrate the stomatal development mediated and maize, Pant dhan-10 rice seedlings survived in all
physiological responses in three varieties of cotton treatments, however, fresh weight (FW) was reduced
grown under well watered conditions at different growth against AsIII (120 µM) ~21%, AsV (120 µM) ~17%,
stage of life cycle (day after sowing). Stomata NaCl (100 mM) ~43% and PEG (20 mM) ~47%, in
development indicates disparities in stomatal traits on comparison to control. However, rice cultivar Saryoo-
both surfaces (lower and upper) accompanied with their 52 seedlings exhibited maximum reduction in FW ~20%
physiological responses. This understanding may help at As(V) (120 µM) and ~38% with PEG (20 mM),
to reveal as to how the long term stomatal development however, rice seedlings could not survive against As(III)
enable plants to adapt under climate change and manage (120 µM) and NaCl (100 mM). Similarly, maize cultivar
its water use strategy. Further molecular analysis will Vievk QPM-9 seedlings survived against all treatments,
be required for elucidation of the mechanism at multiple however, FW was reduced with AsIII (240 µM) ~ 51%,
levels of stomatal response. AsV (240 µM) ~35%, NaCl (200 mM) ~47% and PEG
Key words: Cotton, Physiological responses, Stomatal traits, (40 mM) ~63%. However, Vivek hybrid-43 maize
Temporal development. seedlings could not survive against AsIII (240 µM),
NaCl (200 mM) and PEG (40 mM) treatments. Overall,
SVII/P-41 result indicates that maximum FW reduction was
observed in rice Saryoo-52 and minimum in Pant dhan-
Screening of Sensitive and Tolerant 10 against AsIII & AsV (120 µM), NaCl (100 mM)
Cultivars of Rice (C3) and Maize (C4) and PEG (20 mM) treatments. Similarly, maize Vivek
hybrid-43 exhibited maximum FW reduction and Vivek
based upon Morphological Response QPM-9 showed minimum FW reduction against AsIII
against Abiotic Stress & AsV (240 µM), NaCl (200 mM) and PEG (40 mM)
treatments.
Ambedkar Gautam1,2*, Kavita Shah2 and Shekhar Key words: Abiotic stress, PEG, NaCl, C3 and C4 plants.
Mallick1
1
CSIR-National Botanical Research Institute, Lucknow- SVII/P-42
226001, INDIA; 2Institute of Environment and Sustainable
Development (IESD), Banaras Hindu University, Varanasi- Difference in Leaf Proteome Response
221005, INDIA, Email: shekharm@nbri.res.in of Winter Wheat to Interactive Effect
The present study was conducted to identify a of Elevated CO2 and EDU against
pair of sensitive and tolerant cultivars of rice (C3) and
maize (C4) based upon morphological response against High Ambient Ozone
abiotic (salinity, drought and metalloids) stress. These
Vivek K Maurya 1,2 *, Nalini Pandey 2 and Vivek
stresses disrupt the biochemical and physiological
Pandey 1
processes, leads to reduce growth and yields of the
1
plants. The cereal crops such as rice, wheat, maize, Plant Ecology and Environmental Science Division, CSIR-
barley, sorghum, millet, oat and rye crops are cultivated National Botanical Research Institute, Lucknow-2260012,
worldwide. Particularly, in India, maize is the third most INDIA; 2Department of Botany, Lucknow University,
cultivated cereal crop after rice and wheat. Rice and Lucknow-226007, INDIA, Email: vivekthevivu@gmail.com
maize are classified as C3 and C4 plants, respectively, Concentration of ground level ozone ([O3]) has
on the basis of their anatomy and physiology. These increased twice than preindustrial times and still
plants may also differ in their defense mechanism continuously increasing in northern hemisphere. After
against abiotic stress. In present study, ten cultivars of Bangladesh (21.3%), India (20.2%) is among the top
maize and rice plants each were examined against ten most ozone populated countries worldwide during
given special attention and promoted only after detailed untreated plants under stress conditions. The high
studies. concentration of gold nanoparticles affected these
Key words: Nanoparticles, Polypedates maculates, Enzyme parameters in negative manner.
activities. Key words: Tobacco, Heavy metal, Toxicity, Gold
nanoparticle, Stress conditions.
SVII/P-44
SVII/P-45
Synthesis of Gold Nanoparticles from
Leaf Protein of Nicotiana tabacum Identification of Arsenic Sensitive and
and their ROS Scavenging Responses Arsenic Tolerant Rice Varieties on the
under Stress Conditions Basis of Early Seedling Growth
Responses
Mohammad Israil Ansari*1 and Syed Uzma Jalil2
1
Amity Institute of Biotechnology, Amity University Uttar Poonam Yadav1, Sudhakar Srivastava1 and Madhu
Pradesh, Lucknow Campus, Lucknow, INDIA; 1Department Babu Pulagam2
of Botany, University of Lucknow, Lucknow, INDIA, Email: 1
Institute of Environment and Sustainable Development,
ansari_mi@lkouni.ac.in, ansari_mi@hotmail.com Banaras Hindu University, Varanasi-221005, INDIA;
2
Heavy metals induce serious contamination Directorate of Rice Research, Rajendranagar, Hyderabad,
Telangana, INDIA, Email: sudhakar.srivastava@gmail.com
because of high toxicity. This is transmitted through
the food chain of ecosystem. Heavy metals are naturally The threats of arsenic (As) hazards are known
occurring and industrial activities as a result drastic globally including several states of India. Arsenic
environmental pollution. Plants accumulate metal ions accumulation in rice (Oryza sativa L.) grains is a
that will distribute all over plant as a result will disturb serious issue as it affects health of millions all over the
cellular ionic homeostasis. The stress condition imposed world. It is known that rice varieties differ in their
by the impact of metal and non-metal oxide tolerance to As and in their ability to accumulate As in
nanoparticles over plant systems enhances the synthesis grains. Hence, identification of appropriate rice
of reactive oxygen species (ROS), resulting in oxidative varieties showing low grain As accumulation is a
damage at cellular level. The objective of this study feasible approach to tackle the problem. In the present
was to synthesize the gold nanoparticles from the leaves work, rice screening experiments were conducted to
protein of tobacco and reactive oxygen species (ROS) compare the performance of 64 rice varieties against
scavenging activity on plant after exposure to different As stress (25 and 50 µM). Control and treatments were
stresses. We have treated tobacco plants different run in parallel for each set of experiments. The
concentrations of synthesized gold nanoparticles and performance of rice varieties was assessed on the basis
examined physiological as well as biochemical changes. of seed germination and early seedling growth. Seed
The biochemically synthesized gold nanoparticles germination was recorded when both plumule and
exposure significantly increased the seed germination, radical were grown more than 2mm from the seed coat.
root, shoot growth and antioxidant ability of plants Root-shoot length, percent change in root-shoot growth
depending on bioengineered gold nanoparticles and seedling vigour, were measured after 7 d. On the
concentrations. Low concentrations of gold basis of above data analysis, two most sensitive varieties
nanoparticles boosted growth by approximately 50% (CO-50 and Shobhini) and tolerant varieties (Pooja and
and significantly increase in photosynthetic parameters Vivek dhan) were identified. These varieties can be a
such as total chlorophyll content, membrane ion leakage basic tool for further research in future.
as well as malondialdehyde content with respect to Key words: Arsenic, Rice, Sensitive, Tolerant.
SVII/P-46 SVII/P-47
SVII/P-48 SVII/P-49
Session VIII
Contemporary Issues in Environment and Plant
antimicrobial, cytotoxicity, anti-inflammatory, and technologies. Both approaches have their merits.
responses against selected cell lines-of the crude However it has now become increasingly clear that
alcoholic extracts of the representative species of the disturbing the inherent synergism of a plant metabolome
genera Pandanus, Freycinetia, and Benstonea. could be one of the main reasons for loss of biological
Key words: Pharmacology, Pandanaceae species, Philippines. activity in previous bioassay-guided studies on medicinal
plants. In this regard, systems biology, in particular
SVIII/L-2 metabolomics/metabonomics, will have greater promise
in our efforts to gain a deeper understanding of a plant
A Systems Biology Approach in metabolome effects on a disease state or on other
biological perturbations. This paper will present some
Research on Medicinal Plants: From of our findings and progress made using both
Phytochemistry to Metabolomics approaches with selected examples.
Key words: Medicinal plants, Metabolomics,
Khozirah Shaari
Phytochemistry.
Laboratory of Natural Products, Institute of Bioscience,
University Putra Malaysia, 43400 Serdang, Selangor, SVIII/O-1
MALAYSIA, Email: khozirah@upm.edu.my
Medicinal plants have been investigated and Assessment of Protein Content and
utilized to alleviate disease since early human history. Antioxidant Activity in the Waste
Extracts and infusions containing natural products from Pupae of Mulberry, Tasar and Muga
plants have historically been a major source of
pharmaceutical ingredients, often comprising mixtures Silkworm
of several bioactive compounds with complex
synergistic effects. Their dominant role in modern Ashmita Ghosh 1 , Mrinal Ray 1 , Debnirmalya
medicine is evident in the approximately 60% of Gangopadhyay 1 *, Suchisree Jha 2 and Palash
anticancer compounds and 75% of drugs for infectious Mandal
diseases that were either natural products or natural 1
Department of Sericulture, Raiganj University, Raiganj, Uttar
product derivatives. Despite a declining interest in the Dinajpur, 733134, West Bengal, INDIA; 2Department of
screening of natural products by the pharmaceutical Botany, University of North Bengal, Darjeeling, 734013, West
industry, natural products have continued to be Bengal, INDIA, Email: deb_ganguly2003@yahoo.com
significant source of drugs and leads. The tropical The waste pupae of the silkworm such as
rainforest is a source of unimaginable chemical diversity mulberry (Bombyx mori), tasar (Antheraea mylitta)
for drug discovery. Recognition that this valuable and muga (Antheraea assamensis) generated from
biological diversity is rapidly diminishing is fostering a the silk reeling industry can be a substantial source of
renewal of interest in natural products research. Over protein. In the present study, the waste pupae of
the past twenty years, our group has been focusing on mulberry, tasar, and muga collected from the silk reeling
the isolation and identification of bioactive compounds industry were defatted, crushed into powder and
from the tropical rainforest plants of Malaysia. For analyzed through different biochemical methods to
most part of our research, the emphasis has been on assess protein content and antioxidant activity. The
inflammation, cancer and diabetes mellitus. Recently, present study revealed highest content of protein in
we have also adopted metabolomics approach in the the pupae of tasar (189.4 ± 0.5 mgg-1 ) followed by
hope of obtaining a more holistic understanding of the muga (165.6 ± 0.92 mgg-1) and mulberry (158.2 ± 0.37
biological properties of a medicinal plant or herb. Our mgg-1). The antioxidant activities of the samples were
research endevours which began with a mainly determined by using standard antioxidant assays like
reductionist perspectives, in accordance with the then DPPH, ABTS, superoxide and metal chelating assay.
popular drug discovery approaches used by big pharmas The methanolic pupae extract (MPE) of muga and tasar
and academic laboratories all over the world, has now showed high DPPH and ABTS scavenging activity with
mellowed into a more holistic approach, utilizing ‘omics’
IC50 value of 163.11 and 63.69 ìg mL-1 respectively. products T0 were studied in which Protein, Iron and
On the other hand, the MPE of mulberry showed high Vit C, content were 11.35 g-100g, 10.54 mg-100g and 27.87
metal chelating and superoxide radical scavenging mg-100g respectively. Thus there was a remarkable
activity with IC 50 value of 1.01 and 0.86 ìg mL-1 increase in the nutritional contents of the value added
respectively. The study demonstrates that the waste products.
silkworm pupae rich in protein content may serve as a Key words: Hedonic rating, Nutrients deficiency, Value added
potential source of natural antioxidants for the product, Nutritional composition.
development of animal feed products.
Key words: Antioxidant, Protein, Silkworm pupae, Animal SVIII/O-3
feed product.
Conservation of Herpetofauna in
SVIII/O-2 Cachar District of Assam: Challenges
Product Development and Sensory and Opportunities
Evaluation of Value Added Food Sibam Sarkar and Mithra Dey*
Products made by Incorporating Dried Department of Ecology and Environmental Science, Assam
Murraya ioenigii (Curry Leaf) Powder University, Silchar -788011, Assam, INDIA, Email:
mithradey@gmail.com; shibamsarkar831@gmail.com
Mukta Singh* and Khushaboo Prajapati Recent research has shown that reptilian and
Department of Home Science, M.M.V Banaras Hindu amphibian population worldwide are on a sharp decline
University, Varanasi-221005, INDIA, Email: with approximately 32.5% and 22% of amphibian and
drmuktasingh@gmail.com reptilian species being endangered. Herpetofauna are
Murraya koenigii (Curry Leaf) is one of the important group of vertebrates and play significant role
most common and popular plant grown in South India in the ecosystem. Amphibians are biphasic in lifestyle
but in North India still neglected by people in their and depend on both aquatic and terrestrial ecosystems
consumption pattern. In view of this, an attempt was for their larval and adult phase of life. Herpetofauna-
made to utilize (dried curry leaf powder) to develop occupy an important place in food chain being both
Iron and Vitamin C rich value added products for people predator and prey and form important vertebrate
suffering from nutrient deficiency. The present study biomass in the ecosystem. Cachar is one of the three
was undertaken with an aim to develop value added districts of Barak Valley; the other two being Hailakandi
products (Bengal gram flour sweets) incorporating dried and Karinganj, located in Southern Assam. Barak
curry leaf powder with other ingredients. Three Valley Region of Northeast India is a part of the Indo-
samples were prepared with different incorporation of Burma hotspot, which is one of the 35 global biodiversity
dry curry leaf powder T1:5g, T2:10g, T3:15g and one hot spots and has myriad freshwater ecosystems along
control sample T0 was prepared without incorporation with thick tropical vegetation which offer excellent
of dry curry leaf powder. Hedonic rating was used for habitats. However, herpes have not commanded much
sensory evaluation of value added products. attention and little is known about their ecology and
Incorporation of dried curry leaf powder 5 g was highly natural history. During the present study 16 species of
accepted in sample T1 of Bengal gram flour sweets. anurans (Class: Amphibian) and 24 species of Reptiles
The shelf life studies revealed that the value added (23 species of Order Squamata and 1 species of Order
products stored in glass containers covered with Testudines) were recorded. The study was carried out
aluminium foil had better shelf life. Nutritional from January, 2016 to July, 2018 in Innerline Reserve
composition of value added products T1 were studied Forest (24°35’36.47"N, 92°46’57.47"E), Rosekandy
in which protein, iron and Vit C content were 12.51 g- Tea Estate (24°41’40.12"N,92°41’17.34"E), Silcoorie
100g
, 13.84 mg-100g and 33.87 mg-100g respectively. Tea Estate (24°41’5.55"N, 92°46’18.53"E) and in
Nutritional compositions of control value added Assam University, Silchar Campus (24°41’12.70"N,
92°45’4.18"E ). The economic activity is mainly tea
Key words: Growth retardants, Rare sugar ester, Gibberellin, greenest metro cities of India owing to its green belt
Biological activity, Environmental Friendly. planted along the roadsides, its green zone in the heart
of the city and plantation across the city which was a
SVIII/P-6 visual treat from above; and very soon it appeared in
the list of most polluted cities of the world. The PM2.5
Vermicomposting: Technology level in the air of Delhi crossed all the limits and reached
towards Green Revolution a level which is more than 7 times than WHO safe
levels. The State and the Central government reacted
Bhawna Sharma in the form of series of rules and bans to this grave
Department of Botany, Shri Shakti Degree College, pollution problem. The old diesel vehicles were banned
Sankhahari, Ghatampur, Kanpur Nagar-209206, INDIA, Email: to enter the city, ban on registration of new SUVs, ban
bhawnasharma2468@gmail.com on burning biomass in and around Delhi, odd-even rule
and many such regulations were implemented in an
Domestic solid waste disposal is a matter of every
attempt to control the situation. The improvement in
day discussion at local and global level yet the problem
the Delhi’s air quality could not be visualised by
evades solution. The technique of vermin-culture, if
scientists. A number of other questions arose such as
utilized by every homemaker would certainly reduce
are the existing rules and regulations not effective?
the serious environmental and health problem the world
Were the other factors like overpopulation,
over. There is an urgent need to develop proper waste
industrialisation or climate playing a major role? Are
management technique for recycling of organic waste
these factors easy to control under a short period of
created from kitchen, farms and water bodies. It is
time, say 15 days as allocated for odd-even rule? So
estimated that in cities a human being creates about
we go back to the old existing policies, planning and
0.4kg waste per day. Thus by year 2000, when there
legislation which may have been playing an underlying
will create city refuse of about 44 (0.4 kg X 300m X
role and their implementation or non-implementation
365 days) million per year. The present practice is just
have worsen the situation over the years. This paper is
a way to remove dispose-off garbage, no matter where
an attempt to look into the laws and regulations adopted
it put off, but actually a standard method of vermin-
by the government in the time of crisis and what would
composting technique is required there. The process
have been the situation if the existing policies were
of vermin-composting requires the individual to collect
actually followed.
the kitchen and garden waste in a container rather than
disposing it. So, the vermin-culture is an environmentally Key words: Air pollution, PM2.5, environmental laws, Delhi
beneficial technique and it requires little investment of pollution.
time and some inexpensive materials.
SVIII/O-8
Key words: Kitchen waste, Indoor environment, Eco-friendly,
Worms. To Study the Air Pollution Caused by
SVIII/O-7 Informal E-waste Burningand its
Effect on Human Health
Legal Perspectives of Control on Air
Pollution in Delhi and other Metro Anju Chauhan1,2*, Charu Gangwar1,2, Atul Kumar1,2,
Juhi Khan1,2, Ajay Kumar1,2, Garima Varshney1 and
Cities Anamika Tripathi1
1
Deepika Pandey Pollution Ecology Research Laboratory, Department of
Botany, Hindu College, Moradabad, INDIA; 2School of
Amity School of Earth and Environmental Sciences, Amity Science, IFTM University, Moradabad, INDIA, Email:
University Haryana, Gurugram-122413, Haryana, INDIA, anjuchauhan696@gmail.com
Email: dpandey@ggn.amity.edu
Informal e-waste burning in Moradabad is one
Almost ten years back Delhi was one of the of the major source of increasing toxic heavy metal
concentration in Environment. Present study was aimed crop productivity and carbon sequestration in soil. Yard
to investigate the air pollution and its relation to human waste biomass samples were carbonised with limited
health risk. Therefore, three monitoring sites (SI, SII supply of air in a mufûe furnace at different temperature
and SIII) were selected on the basis of different (300-500°C) and residence time (30-120 min). The
activities in the respective areas. Air samples were resultant biochar was characterized for labile C and
collected with the help of RDS and then subjected to stable organic matter yield index (SOMYI). With
heavy metal analysis by ICP-OES. One of the increase in temperature, biochar yield decreased,
objectives of the study to assess the level of heavy whereas the stability increased. SOMYI was
metal concentration (ICP-MS) in blood of studied calculated from the biochar yield and stable organic
population from different monitoring sites. Results matter content. The carbon liability index of biochar
indicated that except SI, all sites had significant level decreased with increasing temperature and duration
of PM10 and its heavy metal content. Therefore, local of pyrolysis. The yield of stable organic matter
residents had various levels of heavy metal decreased at temperature >300°C. The optimum
concentrations in their blood. The study concluded that condition was found to be heating the yard waste at
exposure to high level of air pollution, containing toxic 300°C for 30-40 min. As the biochar is to be used for
metal such as Pb, Cu, Cr, Ni and Zn, released from e- soil amendment, stable organic matter (SOM) yield is
waste burning activities, resulted into significant health important, rather than the SOM content. TGA and
risk for the exposed human population. On the basis of FTIR studies showed that yard-waste biochar prepared
above it is concluded that environment of Moradabad at 300-350°C has aromatic functional groups and the
City should be closely monitored by government yield of stable carbon is higher. Thus, we recommend
agencies on routine basis, particularly for its metal a low temperature moderate pyrolysis (300-350°C, 30-
content in order to safeguard the health of the population 40 min) condition for preparation of yard-waste
exposed in general and e-waste workers in particular. biochar.
Key words: E-waste Burning Activity, Industrial Activity, Key words: Biochar, Yard waste, Labile carbon, Stable organic
PM10, Blood, Heavy Metal, Human Health, Air pollution. matter yield index.
SVIII/O-9
SVIII/O-10
Stable Organic Matter Yield Index of Characterization and Adsorptive
Biochar Prepared from Yard-Waste Removal of Phenol Red from the
Sangeeta Mukhopadhyay* and R.E. Masto Water using Green Synthesized Iron
Industrial Biotechnology and Waste Utilisation Division, Nano Particles
CSIR-Central Institute of Mining and Fuel Research
(Digwadih Campus), Dhanbad-828108, INDIA, Email: Shalu Rawat1*, Jiwan Singh1, Samiksha Sikarwar2
sangeeta.dccs@rediffmail.com, mastocfri@yahoo.com and B.C. Yadav2
Agricultural waste, crop residues, garden and 1
Department of Environmental Science, Babasaheb Bhimrao
yard wastes are increasing at an alarming rate due to Ambedkar University, VidyaVihar, Raebareli Road, Lucknow-
increase in population. It is reported that about 600 226025, INDIA; 2Department of Physics, Babasaheb Bhimrao
MT of wastes have been generated in India from Ambedkar University, VidyaVihar, Raebareli Road, Lucknow-
agricultural sources alone. All these organic wastes 226025, INDIA, Email: shalurawat200@gmail.com,
jiwansingh95@gmail.com, samiksha9.2009@gmail.com,
create environment pollution. There is a vast potential
balchandra_yadav@rediffmail.com
for production of biochar from organic wastes, which
is otherwise left unutilized or burnt. Moreover, burning Synthesis of nanoparticles via green route
of these wastes causes significant degradation of soil, reduces adverse effects of harmful chemical used in
and emission of greenhouse gases. Biochar is produced chemical synthesis of nanoparticles. Green synthesis
by heating organic waste under limited supply of air. of nanoparticle is an advance and eco-friendly
Biochar has fertilizer value that improves soil quality, technique. In this study extract of waste tea was used
as reducing agent for the synthesis of iron nanoparticles. effects such as radiation-induced cancer, for which
The synthesised iron nanoparticles were characterized there is a certain risk even at low doses of radiation.
by UV-Visible spectrophotometer, scanning electron This risk cannot be completely prevented but can only
microscope (SEM), energy dispersive x-ray be minimized. Therefore, the balancing of benefits from
spectroscopy (EDX), Brunauer-Emmett-Teller (BET), nuclear and radiation practices against radiation risk
particle size analyser, Zeta potential, X-ray diffraction and efforts to reduce the residual risk has become a
(XRD) and Fourier Transform Infrared Spectroscopy major feature of radiation protection. In this paper, we
(FTIR). The average particle size of the nanoparticles shall be looking at the precautionary measures for
was found 98.79 nm. Synthesised iron nanoparticles protecting life, properties and environment against
showed maximum weight percentage of iron 69.07% ionizing radiation.
in elemental composition analysis by EDX. Synthesised Key words: Radiation, Alpha particles, Beta particles, Gamma
iron nanoparticles were used as adsorbent for removal rays, X-rays.
of phenol red dye from aqueous solution. Effect of
different parameters on the adsorption of phenol red SVIII/O-12
was also investigated. Tea waste iron nanoparticles
(TW-FeNPs) effectively adsorbed phenol red from Impact of Healthcare Waste Management
aqueous solution. Maximum adsorption capacity (95.08 on Environmental Health Disaster
mg/g) of TW-FeNPs was found at pH 8 and at room
temperature 25ÚC. The adsorption of phenol red was Salil Chaturvedi 1 , Marisha Rai 2 and Rachna
decreased with increasing temperature of the process Chaturvedi3
that showed that the adsorption of phenol red with TW-
1
FeNPs was an exothermic process. Shyma Prasad Mukharjee (Civil) Hospital, Lucknow-226001,
INDIA; 2Balrampur Hospital, Lucknow, INDIA; 3Amity
Key words: Green synthesis, Adsorption, Tea waste, SEM, Institute of Biotechnology, Amity University Uttar Pradesh
BET. Lucknow-226028, INDIA, Email: drrachnachaturvedi
@gmail.com
SVIII/O-11
Environment hazard disaster due to health care
Effect of Ionizing Radiation on Human waste management is recognized as the worldwide
problem. Globally, it is observed that health care waste
Health management is underfunded and poorly executed. The
collective harmful contagious and additional unsafe
Ashish Chaturvedi
properties of medical waste characterize a significant
King George’s Medical University, Department of Pathology, environmental and public health threat. Health care
Lucknow-226007, INDIA, Email: ashishchaturvedi.jr.sc waste is created by different health care activities. It
@gmail.com includes needles, body fluids, body parts,
The effects of radiation were first recognized in pharmaceuticals, radioactive materials, and cytotoxic
the use of X-rays for medical diagnosis, then later with drugs which are generated by health care
the discoveries of radiation and radioactivity. The rush establishments, health-related laboratories, and health
in exploiting the medical benefits led fairly to the research facilities. There are many potential threats
recognition of the risks and induced harm associated connected with management of health care waste,
with it. In those early days, only the most obvious harm posturing hazards not only to the patients and health
resulting from high doses of radiation, such as radiation care personnel, but also to the community and the
burns were observed and protection efforts were environment health. Nevertheless, hospital waste is
focused on their prevention, mainly for practitioners typically characterised rendering to the hazard it
rather than patients. Although the issue was narrow, conveys. One of the recognized results of overflowing
this lead to the origin of radiation protection as a garbage is the air pollution, which is responsible for
discipline. Subsequently, it was gradually recognized various respiratory infections and other adverse health
that there were other, less obvious, harmful radiation effects as pollutants are absorbed from lungs into other
parts of the body. The poisonous substances in air soil, fish, dung and milk samples were collected in and
polluted by waste include different hazardous gases as around the mine pit and adjacent villages on an
carbon dioxide, nitrous oxide and methane. Health increasing distance of 0.5 to 1 km. These samples were
workers who are managing the waste which is holding acid digested as per American Public Health Association
harmful chemicals and metals may involve in toxic regulation and diluted in distilled water. The prepared
exposure. Therefore it is advisable that disposal of samples were analyzed in ICP-OES and AAS to
hazardous health-care wastes must need distinctive care quantify the concentration of heavy metals. The
because it can create major health hazards, such as comparison was made between the bioaccumulations
Hepatitis B and C, through wounds caused by discarded of heavy metals in plant, soil, fish, dung and milk samples
syringes. It must be taken in consideration that sharps through ANOVA statistical analysis and the rate of
used needles, syringes, or other contaminated should transfer from each soil to plant and higher trophic level
not be mixed in common waste disposal or recycle bin was established by the regression equation. Our result
because by this much of practice the entire waste will reflect that due to coal mining carried out in the past
become infectious. Some facilities even provided should and maybe mineral mining in Talcher might be
include special vehicles and protective devices to responsible for heavy metal accumulation in the soil.
dispose, handling or transport the health care waste Heavy metals accumulated in plants enter the food
products. Disposing of waste has enormous chain and cause metabolic dysfunction in animals along
environmental hazardous impacts and can cause serious with blotting and stunted growth in plants. The impact
problems. In near future this management of health assessment monitoring would help to make changes in
care should be taken seriously to minimize the the mining process that will help in reducing heavy metal
environment hazard disaster. leaching by finding sensitive spots in the mining area
Key words: Environment health disaster, Health care waste, and generate awareness for using heavy metal
Public health, Diseases. contaminated
Key word: Bio-magnification, Spectroscopy, Trophic-level,
SVIII/P-1 Watershed, Mineral leaching
functions, isoelectric point, molecular weight and amino environment and living organisms, the paper suggests
acid sequences, PR proteins are grouped into 17 certain biological management practices and eco-
classes. Few examples of PR proteins are antimicrobial friendly approaches which could be a better option for
peptides, oxidoreductases, hydrolytic enzymes, the farmers. So experiments were conducted to
proteinases and proteinase inhibitors etc. The present evaluate the effectiveness of four wild variety medicinal
paper focuses on PR9 protein family present in oilcakes against root knot nematodes infecting tomato
Solanum lycopersicum (SlPR9). PR9 (Peroxidases) in pot cultures. The experiment comprised of six
proteins catalyze cross-linking of macromolecules in treatments in order of T1 = Neem cake @ 100 gm-²,
the plant cell wall. They also produce free radicals like T2 = Mahuacake @ 100 gm-², T3 = Karanja cake @
H2O2 against a wide range of pathogens. The present 100 gm-², T4 = Kusumcake @ 100 gm-², T5 =
study aims to analyze the cis-elements present in the Carbofuran standard check @ 0.3g a.i.m-², T6 =
promoter regions of SlPR9 followed by its gene Untreated check. Each treatment was replicated thrice
expression analysis.SlPR9 was found to be down- following complete randomised design. The tomato
regulated in all states except in inflorescence visible plantlets variety Pusa ruby was transplanted to the
stage followed by main shoot growth. Protein structure pots. At one week after transplanting, nematodes were
of SlPR9 was modeled using MODELLER followed inoculated into the pots. Observations were taken at
by docking with various phytohormones. 45 days of inoculation which indicated that all
Key words: Peroxidases, Solanum lycopersicum, Promoter, treatments increased the plant growth parameters and
Docking reduced root knot nematode population over check.
The result revealed that the pot treated with neem cake
SVIII/P-3 showed improved plant growth rate and reduction in
nematode infection as compared with other treated
A Comparative Study on the Effects plants. However the present investigation needs testing
of Four Wild Variety Medicinal Oil of treatments in micro plots / field condition for more
concrete results.
Cakes against Root Knot Nematode
Key words: Meloidogyne incognita, Tomato, Oil cakes,
Infecting Tomato Carbofuran.
under below poverty line resulting in malnutrition. (signifying availability in food) and straw(signifying
Utilization of indigenous vegetables can help in availability to milk animals) over full growing season
mitigating food security issues and addressing of rice-wheat for two consecutive years (2013-2014)
malnutrition. Carrot (Daucus carota L.) is a well- in an alkaline soil. Treatments included: recommended
consumed food crop in India. It can be consumed raw, fertilizer doses (F: N: P: K: Zn 180:26:42:7 kg ha-1)
cooked and in processed form. Sixteen Indian carrot compared with reduced fertilizer use (~ 50% cut) but
accessions were analyzed for variation in root size, dry supplemented with organic inputs, namely legume (LE;
matter, chlorophyll content, total soluble protein content, Vigna radiata), green manure (GM; Sesbania
total phenolics, total soluble sugar content and mineral aculeata), farmyard manure (FYM), wheat straw
compositions. Among carrot accessions, significant (WS), and rice straw (RS). The soil stock of Zn was
differences (p<0.05) were obtained with respect to total highest in case of F (fertilizers only) where ZnSO4
phenols and total soluble sugars. Purple coloured fertilizers were applied than organic management.
accessions were found to be a rich source of calcium These trends substantiate stray evidences that Zn
and phenols with a very high antioxidant activity. Yellow fertilizer application could be helping in building up Zn
coloured accessions were rich in sugar content. This content of soils to the level of a pollutant in the western
study revealed that different coloured carrots have their Indo-Gangetic plain (IGP). Overall, Zn availability in
own distinctive nutritive quality. Roots of carrot contain rice and wheat through organic fertilizer application
a considerable quantity of essential nutrients and can was 12.45 and 16.87 µg cm-2 respectively, which was
be a useful source of minerals for a population suffering comparable to the availability provided by fertilizer
from hidden hunger. Indian population is mostly application. In terms of availability of Iron, manganese
consuming red and oranges carrots. It is required to and copper, the integrated management with organics
increase awareness, production and consumption of and cut down fertilizer application proved better than
yellow and purple coloured underutilized carrots, as fertilizers only. The availability was higher in straw than
they are a rich source of nutrients and can help to grain and exhibited the following order: Fe>Mn>Zn>Cu.
combat malnutrition. This could be particularly important for areas where
Key words: Carrot, Hidden hunger, Bioavailability, Nutrition, straw is fed to milch animals. Organic treatment for
Minerals. nutrient management in soils indicates very effective
method of managing plant, human and animal micro-
SVIII/P-5 nutrition as well as to control fertilizer pollution.
Key words: Crop residues, Organic manures, Micronutrients.
Micronutrient Availability and
Distribution in Soils Amended with SVIII/P-6
Crop Residues and Organic Manures Development Projects on Silk
Deepika Rajwar* and Ajay Kumar Bhardwaj Production in North East India
Central Soil Salinity Research Institute, Kachhwa road, Suman Das1*, Mrinal Ray2 , Ashmita Ghosh2 and
Karnal-132001, Haryana, INDIA, Email: deepika.rajwar@
Gangesh B. Singh3
gmail.com; akbhardwaj@gmail.com
1
Department of Handloom, Handicrafts and Sericulture,
Micronutrients are important components of Sericulture Training Institute, Tripura, INDIA; 2Department
human diet. The intensive cropping systems which are of Sericulture, Raiganj University, Raiganj, Uttar Dinajpur-
being followed nowadays are depleting micronutrients 733134, West Bengal, India; 3Central Silk Board, R.E.C.,
from soils. Thus, their amount in our diet has decreased Agartala, Tripura, INDIA, Email:suman.agribiotech@
significantly. In a 13 year long experiment with gmail.com
fertilization treatments, ion exchange resin membranes
India has a long history of silk production and at
were used as plant root simulators to determine
present ranks second largest producer in the world after
availability of micronutrients and their distribution in
China accounting for just five percent of the global silk
soil solution (signifying plant availability), grain
market. The North Eastern Region of India is endowed key role in the ecosystem, utilizing both terrestrial and
with unique agro-climatic conditions suitable for aquatic environment. Being both predators and prey,
practicing all the four sectors of sericulture, namely they are essential components of the food web and
mulberry, eri, muga and tasar contributing about 21% play a crucial role in maintaining a healthy ecosystem.
of the total silk production in the country. With the prime Worldwide, 32.5% and 22% of amphibian and reptilian
objective of promoting the first 3 major sectors in the species respectively, are endangered, and current
region, the Government of India introduced Integrated trends suggest even more species could become
Sericulture Development Project (ISDP) under the threatened in near future. The present paper gives an
North Eastern Region Textile Promotion Scheme overview of the herpetofauna in Tripura. The state of
(NERTPS) which includes the different objectives like Tripura (named after the Hill Tippera) is located in the
assessment of component-wise impacts, issues etc., north-eastern region of India. Tripura consists of eight
assessment of present implementation practices, districts (North Tripura, Unokuti, Dhalai, Khowai, West
marketing mechanisms etc. to counter China’s growing Tripura, Sepahijala, Gomati and South Tripura). Several
silk production. Indian government has invested Rs. locations of the state were selected for the present
690 core in projects in 24 districts under the North East study. For identification morphological study along with
Region Textile Promotion scheme. Under these photography was done. After identification all
schemes, six projects are running in Assam, one in specimens were released. A total of 53 species of
Sikkim, two each Meghalaya and Manipur and rest in herpetofauna (amphibians and reptiles) belonging to 42
other states. Eri silk production reached 3,600 tons in genera and 17 families were recorded; 11 species of
2016-17. The detailed knowledge about the integrated amphibians belonging to 9 genera and 6 families; 10
sericulture development projects and intensive bivoltine species of lizards belonging to 9 genera and 4 families;
sericulture development projects of North East has been 28 species of snakes belonging to 20 genera and 5
possible with broad-based support and co-operation families and 4 species of turtles and tortoises belonging
from various stakeholders. (Note: NERTPS - North to 4 genera and 2 families. Considering the threats it is
Eastern Region Textile Promotion Scheme, ISDP - necessary to design strategies for conservation and also
India introduced Integrated Sericulture Development maintain a balance between economic and
Project) developmental activities and the environment.
Key words: Sericulture, Projects, North-East India, Eri, Muga. Key words: Tripura, Herpetofauna, Amphibians, Reptiles,
Threats, Decline, Conservation.
SVIII/P-7
SVIII/P-8
Documentation of Herpetofaunal
Species and Need for their Is Nitrogen the Next Carbon?
Conservation: A Study in Tripura, India Viney P. Aneja
Department of Marine, Earth, and Atmospheric Sciences
Sumit Nath and Mithra Dey
North Carolina State University Raleigh, NC 27695-8208,
Department of Ecology and Environmental Science, Assam USA, Email: viney_aneja@ncsu.edu
University, Silchar-788011, Assam, INDIA, Email:
nathsumit389@gmail.com and, mithradey@gmail.com
Just as carbon fueled the Industrial Revolution,
nitrogen has fueled an Agricultural Revolution. The use
The recent increase of threats to amphibian and of synthetic nitrogen fertilizers and the cultivation of
reptilian population in north-east India, such as nitrogen-fixing crops both expanded exponentially
deforestation, climate change and various other during the last century, with most of the increase
anthropogenic activities are creating a serious need for occurring after 1960. As a result, the current flux of
the implementation of conservation policies. For better reactive, or fixed, nitrogen compounds to the biosphere
design of conservation practices, study of herpetofaunal due to human activities is roughly equivalent to the total
population is necessary. Amphibians and reptiles play flux of fixed nitrogen from all natural sources, both on
land masses and in the world’s oceans. Natural fluxes activities in the area. The wetland/aquatictaxa as
of fixed nitrogen are subject to very large uncertainties, Cyperaceae, Apiaceae, Polygonum spp. and
but anthropogenic production of reactive nitrogen has Potamogeton are recorded in good number from lake
increased almost five-fold in the last half-century, and sediments. The drifted taxa such as long-dispersed
this rapid increase in anthropogenic fixed nitrogen has Pinus roxburghii (Pine), Cedrus deodara (Deodar),
removed any uncertainty on the relative importance of Picea (spruce) and Abiespindraw (fir) are recorded
anthropogenic fluxes to the natural budget. The in good percentage. The records of oak (broad-leaved
increased use of nitrogen has been critical for increased temperate taxa) constitutes Quercus, Rhododendron,
crop yields and protein production needed to keep pace Alnus, Betula etc. indicating its provenance from higher
with the growing world population. However, similar altitudes in the vicinity indicating the frost free
to carbon, the release of fixed nitrogen into the natural conditions conducive for such forests. The overall pollen
environment is linked to adverse consequences at local, assemblage is dominated by Semi-evergreen to moist
regional, and global scales. Anthropogenic contributions deciduous vegetation with negligible conifers suggesting
of fixed nitrogen continue to grow relative to the natural moderate to low seasonality and good precipitation in
budget, with uncertain consequences. the region with dry summer months at an altitude of
Key words: Carbon, Environment, Nitrogen. ~670 m a.m.s.l. with winter temperatures ranging
between 0-18°C and summer temperatures between
SVIII/P-9 18-35°C. The study serves as modern analogue for
the appropriate assessment of the Climate- vegetation
Pollen-Vegetation Relationship in equilibrium in the region in order to define the past
climate fluctuations in terms of vegetational evolution.
Surface Sediments Renuka Region, The study also highlights the relative percentage of
Sirmaur, Himachal Pradesh, India pollen preservation in the sediment with reference to
plant frequency in the studied area providing clue for
Anjali Trivedi and Anjum Farooqui assessing the quantitative estimation of pollen the
Birbal Sahni Institute of Palaeosciences, 53, University Road, sedimentary profile.
Lucknow-226007, INDIA, Email: atrivedee@gmail.com, Key words: Pollen, Tress, Himachal Pradesh.
afarooqui@yahoo.com
SVIII/P-10
The Renuka lake and the adjoining reserved forest
area is situated at 30°362 363 Lat. 77°272 303 Long. Fungalflora Associated with Indian and
The surface sediment samples were collected in a
transect from different ecological niches in about 4.0 Foreign Coins and their Potential
square kilometers area to study pollen assemblage and Health Risks
its relationship with the vegetation in the region. The
qualitative and quantitative study revealed the Drishti Navlani*, Aditi Maheshwari, Vidhatri Thakkar,
dominance of arboreals (trees and shrubs) over the Rohit Taunk, Naitik Chaudhary and Arun Arya
non-arboreals (herbs) suggesting forested area. Shorea Department of Environmental Studies, Faculty of Science,
robusta (12-17%) shows its dominance followed by The Maharaja Sayajirao University of Baroda, Vadodara,
Terminalia, Dalbergia sisso, Mangifera indica, INDIA, Email: sarojarun10arya@rediffmail.com
Syzygium cumini, etc. Gardenia and Cassia fistula
Potential role of spread of pathogenic miroflora
are in moderate to low values. Acanthaceae, Fabaceae,
on coins and currency notes has been evaluated in
Murraya koenigii, Lantana camara and Saxifraga
different countries. Notes and currency coins may
sp. are the representatives of under storey shrubby
serve as universal vehicles for transmission of
vegetation. The herbaceous flora are less-diversified
potentially pathogenic microorganisms among persons
and largely constituted of Poaceae whereas,
either directly, through hand to hand contact, or
Asteraceae, Ranunculaceae, etc.. However, the
indirectly, through water and food. The Purpose of
sporadic presence of ruderal plants as Artemisia,
study was to do qualitative and quantitative analysis of
Chenopodiaceae and Brassicaceae depicts the human
microflora associated with Indian and foreign coins. contains atranorin, alectoronic, á-collatolic acid, 4-O-
Results of the study revealed that bacteria were present methylphsodic, and physodic acid. These lichens
in most of the coins more than 40% coins showed monitor air pollution and play vital role in dye, perfumery
presence of fungi like Aspergillus niger, Curvularia and medicinal industries. Samples were collected from
lunata, Chaetomium globosum, Fusarium roseum inroute Chansile, 4 kms on Pinus and its organic extract
and Trichoderma viride. Fungi like Aspergillus was prepared using solvents methanol, ethanol, acetone
nigerand Fusarium roseumc an cause bronchial and ethyl acetate by the soxhlet setup of extraction.
diseases and may act as potential allergens and few of Common negative control used was DMSO i.e.
these are reported to cause skin problems. The Use of dimethyl sulphoxide and specific positive control
digital transaction will reduce such harmful transaction. streptomycine for antibacterial and ketoconazole for
Cleaning the coins with water or UV treatment may antifungal. This present work was promulgated to
be reduce the fungal load. explore the lichen Cetrelia braunsiana with special
Key words: Currency coins, Indian, Foreign, Aspergillus, reference to its antimicrobial testing against pathogenic
Fusarium, Chaetomium. bacteria such as Staphylococcus aureus,
Streptococcus mutans, Agrobacterium, E. coli,
SVIII/P-11 Pseudomonas aeruginosa, Klebsiella pneumonia
and pathogenic fungus Candida albicans, Aspergillus
Bioprospecting Role of Cetrelia niger and Fusarium oxysporium. Maximum inhibition
braunsiana against Pathogenic Micro- zones were observed in methanolic extracts against
bacteria Agrobacterium tumefaciens and
organisms Staphylococcus aureus whereas ethyl acetate
extracts against fungi Candida albicans and
Devashree 1 , Anupam Dikshit 1 and Sanjeeva Fusarium oxysporum and it can be used as a herbal
Nayaka 2 formulation in future use. This will definitely provide a
1
Biological Product Laboratory, Department of Botany, base and for the future perspectives and highlight the
University of Allahabad, Allahabad, INDIA; 2Lichenology need for further studies of this promising source to
Laboratory, CSIR-National Botanical Research Institute, harvest more beneficial in the field of bioprospection.
Lucknow-226001, INDIA, Email: devashreerishabh021
@gmail.com Key words: Symbiotic, Cyanobacterium, Antimycobacterial,
Antiproliferative, Bioprospecting.
Lichens are one of the best examples of symbiotic
association where two dissimilar organisms, a fungus SVIII/P-12
and an alga or a cyanobacterium live together
physiologically synchronized and well intermixed so as Adoption Extent and Horizontal
to form a single biological unit. The lichen substances Spread of Tomato (Lycopersicon
also do have great biological potentials including
antibiotic, antimycobacterial, antiviral, antioxidant, anti- esculentum Mill.) Cultivation through
inflammatory, analgesic, antipyretic, antiproliferative Front Line Demonstration in Eastern
and cytotoxin. In the present study, crude extracts of Uttar Pradesh of India
Cetrelia braunsiana were tested for its antimicrobial
activity. Cetrelia belongs to the lichenized ascomycete P.K. Misra1, P.N. Singh1, S.N. Singh2 and Pardeep
family Parmeliaceae, cetrarioid genera. The name of Kumar3
Cetrelia is a fanciful concoction from Cetraria and
1
Parmelia, in which most of the species had previously Krishi Vigyan Kendra, Varanasi, INDIA; 2Krishi Vigyan
Kendra, Masouda, Faizabad, INDIA; 3Krishi Vigyan Kendra,
been placed. Although there were many reports on the
Siddharthnagar, INDIA; ND University of Agriculture &
study of Cetrelia almost no expert study on Cetrelia Technology, Kumarganj, Faizabad, Email: pkmisra2001
had been conducted in Korea until the macrolichen flora @gamil.com
of South Korea was published there were 8 species of
Cetrelia recorded in Korean peninsula. Thallus The present study was undertaken to find the yield
in this crop. Among additive and dominance rooted among the local inhabitants. Allopathic drugs
components additive component of variance was having adverse effects, the use of ethnomedicinal plants
predominant for all the traits. Thus selection of parts for treating diabetes in form of infusions,
promising lines/plants based on these traits in advance decoctions and powders are in demand. An
generations can lead to get elite line(s). ethnobotanical survey was conducted to identify the
Key words: Alkaloids, Epistasis, Gene actions, Heterosis. medicinal plants being used to treat diabetes in Gajapati
district of Odisha. During the survey, all the seven
SVIII/P-14 administrative blocks such as Paralakhemundi/Gosani,
Gumma, Kashinagar, Mohana, Nuagada, Ramgiri
Effect of Different Soils on Leaf Udaygiri and Rayagada of the district were covered
and the traditional practitioners and local tribes (50-70
Growth of Jatropha: A Biodiesel Plant years age groups) belonging to Savar, Shavarlodha and
Kandha were interviewed to collect information
Sapna La’Verne1,2*
regarding the mode of administration, doses and their
1
Department of Botany, University of Lucknow, Lucknow- efficacy in treating diabetes. Over 62 medicinal plant
226007, INDIA; 2Residence: 13/1/33, Vikas Nagar, Lucknow, species belonging to 42 angiospermic families were
INDIA, Email: sapna.laverne@rediff mail.com. identified used for the treatment of diabetes in the
Soil is a critical part of successful agriculture and district. The most frequently used plants were observed
is the original source of the nutrients in which we use belonging to families: Fabaceae, Rutaceae and
to grow crops. The nutrients move from the soil into Caesalpiniaceae. According to the traditional
plants through absorption and translocation. Soil plays practitioners some plants are quite effective in the
an important role in filtrating and purifying water. management of the disease but still clinical intervention
Nutrient cycling, water regulation and other soil studies are required to provide evidence for a safe and
functions are normal processes occurring in all effective use of plants or plant parts in the treatment
ecosystems. Adequate nutrients result into healthy of diabetes.
plants, some of them are very good source of Key words: Diabetes, Ethnomedicinal, Gajapati district,
economical important constituents. Since carbohydrates Odisha, Traditional practioners, Tribal groups.
synthesis mainly takes place in leaves. It is the size of
leaf which matters the nutritive and other quality of SVIII/P-16
plants including that of Jatropha curcus plant, which
is considered to be an important diesel plant. Defluoridation of Real Groundwater
Key words: Jatropha curcus, Plant leaves, Alluvial, Sample using Two Step Electro-
Mountain, Desert, Red soil.
coagulation
SVIII/P-15
Vishakha Gilhotra* and Manpreet Singh Bhatti
Ethnomedicinal Plants Used by the Department of Botanical & Environmental Sciences, Guru
Nanak Dev University, Amritsar-143005, Punjab, INDIA,
Tribals for the Treatment of Diabetes Email: mbhatti73@gmail.com, vishgilhotra@gmail.com
in Gajapati District of Odisha In the latest report by Centre Ground Water Board
(CGWB), New Delhi, there are many areas in Punjab
Somani Jethi1 and Kunja Bihari Satapathy2
(Amritsar, Bathinda and Tarn Taran), showing
1
S.K.C.G (Auto) College, Paralakhemundi, Gajapati, Odisha, groundwater fluoride content as high as 5 mg/L.
INDIA; 2Centurion University of Technology and Prolonged exposure of fluoride can cause dental
Management, Bhubaneswar, Odisha, INDIA, Email:
fluorosis and osteo-fluorosis. Thus, an effort was made
jethi.somani@gmail.com, kbs_bot@rediffmail.com
to defluoridize real groundwater sample having fluoride
Being a tribal rich district of vast forest cover the content of 4 mg L-1collected from 200 feet tube well in
use of medicinal plants as herbal healers is traditionally Tarn Taran district (31.39555° N 74.62112° E) Punjab,
India. The health effects of fluoride exposure were lower urinary tract infection affects urethra (urethritis)
also observed in local people such as mottling of teeth, or the bladder (cystitis). The increased susceptibility
abnormal tooth enamel and crippling disease indicated of the females to UTIs is associated with anatomical,
fluoride toxicity. Electrocoagulation employing behavioral and physiological factors. Most of the UTIs
aluminium electrodes and response surface occur due to bacterial invasion from the bowel or
methodology (RSM) was used for removal of fluoride vaginal mucosa. Women are at high risk of infection
from water. The effect of treatment time (3-37 min) due to shorter urethra as compared to men which
and current density (10-40 A/m2) on fluoride removal provides an ideal path for invasion of uropathogens. In
efficiency was studied at fixed pH 7.35. Residual addition, during sexual intercourse when a diaphragm
fluoride concentration satisfied BIS standard (< 1.5 mg is used for contraception, and during pregnancy, due to
L-1) at 37 min treatment time and 40 A/m2 of current partial stagnation of the urine from pressure on the
density with energy consumption of 1.64 KWh/m3. To urinary tract increases the susceptibility rate of UTIs.
make the treatment process energy efficient, two step Strains of Escherichia coli are the most commonly
defluoridation process was done at low current density identified microorganisms in the UTI cases. Other
(14 A/m2) for 37 min with the removal efficiency of microbes involved are Enterobacter, Enterococcus,
48%. In the second step, final residual fluoride Klebsiella, Proteus mirabilis, Staphylococcus
concentration was <1.5 ppm with energy consumption aureus etc. The UTIs were diagnosed by clean-catch
of 0.9 KWh/m3. Negative values of zeta potential midstream technique which involves the cleansing of
indicated the OH- surface exchange mechanism of skin and mucous membranes around the urethral
fluoride. Furthermore, to elucidate the mechanism of opening before urination. Urine samples were collected
defluoridation, sludge samples were characterized using in sterile containers and cultured using calibrated loop.
X-ray diffraction analysis, Fourier transform infrared The types of media used for routine culturing includes
spectroscopy (FTIR) and Raman spectroscopy blood agar, MacConkey’s agar and nutrient agar. The
suggested the complex formation between aluminium antibiotic susceptibility of the uropathogens was also
hydroxide/ oxy hydroxide and fluoride. Scanning checked using different antibiotics as per standard CLSI
electron microscope (SEM) indicated the change in guidelines. The most common bacteria identified
surface morphology after addition of fluoride. through morphological and biochemical tests were E.
Key words: Water Pollution, Process optimization, Coli, Klebsiella, Enterobacter, Staphylococcus etc.
Electrochemical treatment, Response surface methodology The increasing bacterial resistance to commonly used
(RSM), Aluminium electrodes. antibiotics emphasizes us to use them judicially.
Key words: UTI; Bacterial invasion; Uropathogens;
SVIII/P-17 Antibiotic susceptibility, Bacterial resistance.
towards sustainable environment-friendly development nutrient contents. Humicil amended and control B.
in the coming years. The basic theme of nanotechnology juncea (L.) fields were maintained at three selected
is to use particles having size in nanometer range for agro-ecosystems (experimental sites (ES) ES1
various applications in medical fields, cosmetics industry, (Varanasi), ES2 (Sultanpur) and ES3 (Gorakhpur) of
and agriculture and food technologies. The benefits eastern Uttar Pradesh, India. We reported that humicil
associated with nanotechnology include among others amendment significantly improved the soil properties
increase in yield and quality of produce in agriculture, by 19.7, 10.4 and 12.3 % increase in moisture content
improved cosmetic products and directed delivery of and 7.4, 6.8 and 3.0% increase in cation exchange
medicines. However, immense focus on capacity in comparison to the control at ES1, ES2 and
nanotechnology in past few decades has led to its ES3 respectively. A 1.4, 1.4 and 1.5 fold increase in
unrestricted development and consequently enormous total organic carbon and 2.8, 2.5 and 2.1 fold increase
use of nanoparticles (NPs). It is considered that NPs in soil available nitrogen were recorded at ES1, ES2
may pose risks to the environment and biological and ES3 sites respectively. Moreover, humicil also
systems. It is also becoming evident that the size, improved the microbial biomass carbon at each site
structure and type of nanomaterials have different with highest 28.3 % increases at ES1. Under practiced
effects on plants and environment. Hence, long term field, soil enzymes showed maximum 3.1, 2.8 and 3.0
life cycle analyses are needed to assess impacts of fold increase for alkaline phosphatase at ES1, ES3 and
nanoparticles. This review presents a brief overview ES2 respectively followed by â-glucosidase and urease
of various applications of nanotechnology and discusses activity. Among all three sites, highest 2.9 fold increase
its positive and negative aspects especially in in yield were found in 75% NPK+25% humicil
agricultural field. The review emphasizes that future amended field at ES1 followed by 2.4 and 2.2 fold
development of nanotechnology must be based on increase at ES3 and ES2 respectively. Humicil also
scientific evaluations of benefits and risks associated enhanced crop nitrogen, phosphorus and potassium
to it in long term. contents with maximum 46.4, 47.0 and 17.7% increase
Key words: Agricultural usage, Growth, Metal oxides, at ES1 along with the better soil sustainability indicators
Nanotechnology, Reactive oxygen, Species. at each site. Thus, humicil is a promising amendment
for improving the agricultural sustainability through
SVIII/P-19 improved soil quality, crop yield and nutritional quality
of B. juncea.
Application of Humicil for Enhancing Key words: Agro-ecosystems Sustainability, Crop yield, Crop
the Soil Quality and Yield of Indian nutritional quality, Humicil, Soil quality.
Mustard Brassica juncea (L.) SVIII/P-20
Rajan Chaurasia*, Rama Kant Dubey and P.C. Endolichenic Fungus: New Horizon for
Abhilash
Biopharmaceutical Development
Institute of Environment & Sustainable Development,
Banaras Hindu University, Varanasi 221005, UP, India, Email: Jyotsna Chakarwarti* and Sanjeeva Nayaka
rajan.chaurasiya92@gmail.com
CSIR-National Botanical Research Institute, Rana Pratap
Agricultural practices may influence soil fertility Marg, Lucknow-226001, Email: j.chakravorty90@gmail.com
as they directly influence the soil biochemical
processes. The largely practiced conventional farming Lichens are the result of symbiotic association
has led to the global deterioration of arable soil. Today, between a fungal and a photosynthesising partner (alga
organic amendments are being promoted for sustainable or cyanobacteria). In addition to these mycobiont, many
cropping and better soil quality. Therefore, two-year non obligatemicrofungi reside in lichen thalli in close
field experiments were conducted with Brassica relationship with the algae, Thesemicrofungi are called
juncea (L.) to examine the effect of humicil “endolichenic fungus” (ELF). Endolichenic fungus are
amendment on soil quality, crop growth, yield and its diverse group of predominantly filamentous fungi that
reside asymptomatically in the interior of the lichen thalli. development. An immense amount of effort is still
Endolichenic fungi are akin to endophytic fungi of required to discover the potential of these bioactive
vascular plants in many aspects. Like the endophytic metabolite producers. As rich sources of unique
fungi they produce an array of secondary metabolites bioactive metabolites, therapeutic agents from ELF may
such as alkaloids, quinones, furanones, pyrones, represent a new horizon for the development of
benzopyranoids, xanthones, terpenes, steroids, peptide biopharmaceuticals.
sand allycyclic compounds. Natural products remains Key words: Endolichenic fungus, Endophyte, Bioactive
a consistent source of drug leads with 40% of new metabolite, Antimicrobial activity.
chemical entities (NCE). It has become imperative to
explore micro organisms for NCEs and lead –drug SVIII/P-21
molecules for the drug discovery. Keeping this in view
bioprospecting of micro organisms is carried out from Impact of Radionuclides around
every possible source, including extreme environments Nuclear Facilities: Radio-Ecological
like ocean beds, geothermal vents, cold desserts etc.,
in search of novel strains with promising and Public Health Approach
bioactivities.During past two decades it has been
observed that much wealth of microbial biodiversity Devi D. Pandey
with novel biochemistry and secondary metabolite Centre for Science Education, (Affi. to VP, DST, Govt. of
production resides in endophytes. So far, numerous India-Delhi VP-UP-0050), Mehndawal, Sant Kabir Nagar-
bioactive molecules have been isolated from endophytic 272171, U.P., INDIA, Email: vatsdd111@gmail.com
fungi. An important step towards tapping their potential Current study revealed that status of
for human welfare including drug discovery and environmental risk and associated risk to public and
sustainable agriculture, it is very essential to isolate proper monitoring on the basis of Bioaccumulation and
endophytes from various ecological niches. Among the genetic toxicological approach mainly on Non-human
endophytes lichen associated fungi are unique biota e.g. Daniorerio, Channapanctatus, Dafniasp and
organisms that have the potential bioactive properties plants mainly spirogyra and lemnamnor and major.
including antibiotic, antioxidant, antiviral, anti- Samples are collected from outside of facilities within
inflammatory, analgesic antipyretic, anti-proliferating 30 km. areas and radioecological studies are carried
and cytotoxic activities. More than 30 endolichenic fungi out with the help of counters mainly alpha and beta
(ELF) have been characterized for their bioactive activities in samples then alpha and beta activities in
compounds, and over 145 novel metabolites have been given target species fallowed by stable and unstable
described so far. Besides metabolites, simple aromatic element profile together by AAS, ICP,LSA etc. Tritium
compounds have also shown high levels of antioxidant activities also monitored with the help of LSA for
activity that might protect against cardiovascular disease assessment of HTO, OBT and Genotoxicity profile.
and certain cancers. Viridins are unique class of sterols During operation of plant HTO evaluated particularly
that are promising bioactive compounds due to their CANDU Reactors. Activities are under limit and
potent inhibitory activities. Bioactive sesterterpenes exposure to public as per as norms given by IAEA,
identified in Ulocladium species not only exhibited AERB etc. bioaccumulation studies show high beta
antimicrobial but also displayed potent cytotoxic activities because of hyper accumulating species like
properties. Ambuic acid, isolated from Pestalotopsis daphnia. MN assay also carried out over Channa spp.,
sp. showed potential antifungal activity. Xnthoquinodins rarely observed because of high tolerant species due
characterized from Chaetomiumelatum exhibited to stress.
strong cytotoxic property against various cancer cell
lines. Recently, there has been tremendous interest in Key words: Bioaccumulation, Channa spp., MN Assay, AAS,
ICP, Beta activities, Radioecology.
use of ELF based metabolites for natural drug
potential sources can lead to the discovery of some bulletin on local and International prices etc. The
novel phytomolecules which can be of great use of promotion of organic agricultures is directly concerned
mankind. with its principles of health, ecology, fairness & care
Keywords: Invasive Alien Species, Tribal Communities, and for promoting organic agriculture in the country
Madhya Pradesh, Gynecological disorders, niche are and niche crops are to be identified and
Ethnopharmacology. demand driven crops should be taken. Besides this, for
the benefit of farmers there is a need of main streaming
SVIII/P-24 the organic agriculture as a sustainable alternative
production system approach and long term studies in
Organic Farming: A Scientific different agro-ecological regions for strengthening the
Approach for Sustainable Agriculture data support system in terms of productivity, soil health
and quality.
in Rainfed Hill Conditions of North-
Key words: Organic Farming, Global market, agriculture,
Eastern Hill region North-Eastern Hill region
of heavy metals. Copper is present in higher wheat cultivars. Lipid peroxidation decreased in EDU
concentration followed by Zinc. Absence of Arsenic treated plants at both the stages while increment in
and Cadmium shows less threat of this plant to human total chlorophyll and carotenoids were more prominent
consumption. at flowering stage. Grain wt plant-1 increased in EDU
Key words: Parkia roxburghii, Thioproline, Condensed treated plants of 9 wheat cultivars. With application of
tannin, GC-MS analysis. linear regression statistics by taking average grain
weight plant”1 as the main parameter, three cultivars
SVIII/P-26 (PBW 502, WH 711 and DBW 17) out of eleven had
higher than average grain weight plant ”1 with all
Assessment of Sensitivities among 11 treatment combinations and can be recommended for
Wheat Cultivars Towards Ambient cultivation in areas having high O 3 level. This
investigation concluded that EDU had protected the
Ozone by Application of Ethylenediu- plants from ozone induced toxicity and also helped in
rea (EDU) in Natural Field Conditions monitoring the differential responses of eleven wheat
cultivars.
Baisakhi Majumder1,2*, Ashutosh K.Pandey1, Elina Key words: Wheat, EDU, Ozone, Antioxidants, Yield.
Oksanen3, Tapan K. Nailwal2 and Vivek Pandey1
1
Plant Ecology and Climate Change Science Division, CSIR- SVIII/P-27
National Botanical Research Institute, Lucknow-226001,
INDIA; 2Department of Biotechnology, Kumaun University, Gentianaceae: Two New Species and
Bhimtal Campus, Bhimtal-263136, Uttarakhand, INDIA; One New Addition to Flora of India
3
University of Eastern Finland, Department of Biology, POB
111, 80101 Joensuu, FINLAND, Email: baisakhi.nbri12@ from Ladakh Trans-Himalaya, India
gmail.com
Mohd Shabir1,2*, Priyanka Agnihotri1, J.K. Tiwari2
Prevailing ambient ozone in India is sufficiently
and Tariq Husain1
high to affect the agriculture productivity hence a great
1
threat to food security and agro-economy. Wheat is Plant Diversity, Systematics and Herbarium Division, CSIR-
2nd most important food crop for Indian population and National Botanical Research Institute, Lucknow-226001,
sensitive to ozone. Chemical ethylenediurea (EDU) Uttar Pradesh, INDIA; 2Plant Systematics Laboratory,
Department of Botany and Microbiology, HNB Garhwal
protect plants specifically against O3 stress and has
University, Srinagar Garhwal-246174, Uttarakhand, INDIA
been utilized in the natural field conditions as a Email: shabir1610@gmail.com,
phytomonitoring agent for assessment of sensitivity and
yield losses in various crops and their genotypes. Trans-Himalaya region usually described as “High
Present study involves screening of 11 cultivars of Altitude cold Desert” lies in Ladakh region in Jammu
wheat (Triticum aestivum) for ozone tolerance with and Kashmir, Lahul, Spiti and Kinnaur in Himachal
EDU treatment at an urban site (CSIR-NBRI) Pradesh and small portion of Uttarkashi and Chamoli
experiencing high atmospheric O3. During the study districts in Uttarakhand. The total area of the cold
ambient O3 concentration was monitored by automatic desert in India is ca 98,660 sq.km, of which ca 82,665
ozone monitor (2B Tech). 300 ppm of antiozonant sq. km (84%) area is covered by Ladakh, which lies
ethylenediurea (EDU) was applied in form of foliar between 32o 50’–37o 20’ N and 72o 30’–80o 15’ E and
spray at an interval of 15 days. Biochemical parameters consists of two districts Leh and Kargil. The area has
such as pigment content, lipid peroxidation, antioxidants been surveyed during the recent botanical explorations
and antioxidative enzymes along with yield parameters in our ongoing revisionary studies on genus Gentiana
such as grain wt plant-1 were measured. During the L. in Indian Himalaya. As a result two new species
study period, average O3 concentration of 8 hr ranged Gentianella tumailica M. Shabir, P. Agnihotri, J.K.
between 44 ppb- 64 ppb. The activities of CAT and Tiwari and T. Husain, and Gentiana pringlei M. Shabir,
antioxidant GSH was significantly higher at flowering P. Agnihotri, J.K. Tiwari and T. Husain, have been
stage in comparison to vegetative stage in EDU treated described as novelty from the region, the former is
named after the type locality Tumail, Kargil district of propagate gerbera for large scale production of
Ladakh, while the latter is named in honor of Dr. J.S plantlets. Seven most attractive and market valued
Pringle, Royal Botanical Garden, Ontario, Canada, the cultivars were selected for this in vitro study. A high
authority of new world Gentian. In addition, Gentiana frequency shoot organogenesis has been developed
aperta Maximowicz, has been reported as a new from inflorescence and peduncle of ex vitro gerbera
distributional record to India from Ladakh, Trans- cultivars grown in CSIR-NBRI polyhouse. Inhibition
Himalaya, India. Few more discoveries and new reports of ex plant regeneration due to phenolic content was
from the area are in pipeline, thus, Trans-Himalayan overcome with the addition of ascorbic acid, cysteine
region in India serve as a favorable habitat for and insoluble PVP to the medium. The conversion
Gentianaceae and a promising area of research in the pattern of floral axis to vegetative axis and the
family on various aspects. The stout and diminutive development of shoots from different florets have been
nature, deeply rooted, reduced stem, very small leaves discussed. Organogenic cultures were obtained in
are some factors, which help it to flourish in the extreme Murashige and Skoog’s medium supplemented with BA
conditions of cold and harsh environment of Trans- and IAA. Regenerated shoots were rooted in medium
Himalayan region. without plant growth regulator and after hardening
Key words: Gentianaceae, Two new species, One new record, grown under net house, where they flowered. It could
Trans-Himalaya, India be inferred from this investigation that gerbera cultivars
‘Dana Ellen’, ‘Salvador’ and ‘Rosaline’ are the best
SVIII/P-28 cultivars to grow in this warm subtropical climate, as
compared to ‘Goliath’, ‘Zingaro’ and ‘Silvester ’,
Selection of Gerbera jamesonii whereas, ‘Sunway’ is not performing well under field
Cultivars for Subtropical Floriculture conditions.
through In vitro Regeneration of Key words: Gerbera, cultivars, ex plant, floral axis,
inflorescence, tissue culture
Shoots - A Laboratory to Field Study
SVIII/P-29
1
Dhermendra Kumar Purshottam* , Ramesh Kumar
Srivastava2 and Pratibha Misra*1 Technology and Training Needs
1
Council of Scientific and Industrial Research-National Assessment of Farm Women in West
Botanical Research Institute, Rana Pratap Marg, Lucknow- Kameng District Arunachal Pradesh
226001, INDIA; 2CSIR-Central Institute of Medicinal and
Aromatic Plants, Kukrail Picnic Spot Road, Lucknow, INDIA
Email: pratibhamisra@nbri.res.in N.K. Mishra, T.S Mishra, A. N Tripathi and V.K
Misra
Gerbera (Gerbera jamesonii, family
KVK West Kameng Dirang Arunachal Pradesh, INDIA; KVK
Asteraceae) is a temperate ornamental plant. Beautiful
Zero, Lower Subansiari Arunachal Pradesh, INDIA
cut flowers of this ornamental plant have high potential
for floriculture industry in India due to its availability in Email: navneetkumarmishra123@gmail.com
various colors and long vase life. Natural propagation The West Kameng District, comes under remote
of this plant is very restricted though viable seeds. Ex areas of Arunachal Pradesh having ample scope and
vitro propagation through suckers /rhizome is also poor opportunity in Agriculture field with very good
to initiate new plants on large scale. A lot of work on conducive climate supportive for all type of Agriculture
tissue culture of gerbera has been done but the selection and horticultural crop as the district has a wide range
of suitable cultivars growing in hotter climates, their of climate (Temperate and Sub tropical) and elevation
regeneration potential and the selection of proper about 250 meters at (Bhalukpong) – 4000 meters at
explants to obtain maximum shoots in minimum period Selapass. The most of the population of the district
is the major concern of gerbera growers. Keeping this about 99% population is tribal among which most of
view in mind we initiated the in vitro practices to them are from poor background having no any technical
Know-how for their livelihood support. In this district capable of As-binding was investigated by FTIR. The
Women population play a significant and crucial role in As-removal by immobilized fungal beads tested in the
agricultural production and development processes. packed columns also. The As-adsorption by biomass
They perform a variety of agricultural operations (qe as mg g-1) were recorded as 59.5 (FNBR_3 and
physically, mostly drudgery filled and manage the FNBR_6), 74.8 (FNBR_13), and 66.3 (FNBR_19) in
livestock production activities. Kitchen gardening and the column and validated by Thomas model. This is
fish farming honey cultivation, spice cultivation, flower the first report concerning the arsenic removal by
cultivation poultry keeping are also the exclusive jobs immobilized biomass of these novel fungal strains from
of women, besides home making. The role of farm aqueous solution both in batch and column studies with
women in agricultural development is substantial and a prospect of their further industrial application.
their involvement in transfer of technologies is Keywords: Biosorption, Arsenic, Immobilized fungal
becoming more and more crucial due to the changing biomass, Sodium alginate, Adsorption isotherm Kinetics.
socio-economic environment. Therefore, it is imperative
to understand the Technology and Training Needs of Application of Silica Enhanced
farm women in the transfer of farm technologies from Growth, Yield and Level of Trace
the laboratories to the land.
Elements in Rice Grain and Reduced
Key words: Technology, Farm Women, Agriculture, West
Kameng district.
Arsenic Accumulation
control. Application of Si enhanced the growth and yield of different oil properties showed: percentage oil yield
of plants under As stress by stimulating synthesis of 32 w/w%, moisture content 0.071%, specific gravity
thiols and activities of antioxidant enzymes, and greatly 0.922, acid value 9.718 meg/g, peroxide value 3.56099
reduced grain As accumulation. The level of trace meg/kg, saponification value 184.11. Bio-fuel properties
elements also increased in the grains of Si treated plant showed: percentage oil yield 76v/v%, moisture content
in presence or absence of As. Foliar application of Si 0.04%, specific gravity 0.762,acid value 1.67 meg/g,
was found to be more effective in reducing grain As peroxide value 7.87meg/kg, saponification value 93.50.
level than soil-Si. Results of the present study showed Key words: Jatropha curcas oil, ransesterification, Future
that application of Si in the form of OSA reduces fuel, Bio-diesel.
negative impacts of As, not only in terms of economic
yields but also by improved grain quality. Antioxidant Stress Response and
Keywords: Antioxidants, Arsenic, Orthosilicic acid, Rice, Micro Nurient Content Availability in
Trace element. Foliar of Leguminous Varieties under
Optimistic Jatropha curcas in India eCO2 Concentration
Hoor Fatima Sonali Mehrotra1,3*, Ashish Praveen1, K.P. Tripathi2
Department of Botany, University of Lucknow, Lucknow- and Pramod Arvind Shirke1
226007, INDIA, Email: hoorfatima136@gmail.com 1
Plant Ecology and Environmental Sciences Division, CSIR-
The worldwide effort to find a sustainable and National Botanical Research Institute, Rana Pratap Marg,
Lucknow-226001, INDIA; 2Botany Department, Dolphin (PG)
environmental friendly alternative to today’s fossil fuel
Institute of Biomedical and Natural Sciences, Manduwala,
dominated energy supply has already resulted in a Dehradun-248007, INDIA; 3Life Science Department,
substantial and rapidly growing demand for bio-fuels. Suddhuwala, Uttrakhand Technical University, Dehradun,
Bio-fuels are fuels produced directly or indirectly from INDIA, Email: sonali.mehrotra412@gmail.com.
organic material which includes biomass of plant
material and animal waste. Jatropha curcas is a Antioxidant stress response and micro-nutrient
perennial, oil-seed bearing shrub originating from content were determined in foliar of Cyamopsis
Central America, and which has now been introduced tetragonoloba varieties RGC 1002 and RGC 1066
to other tropical regions in Africa and Asia. It belongs grown under elevated e[CO2 ] and ambient a[CO2 ]
to a spurge family, Euphorbiaceae. There were pronged concentration (450±50ppm and 300±50ppm
benefits of this Jatropha as it would help in greening respectively) maintained in FACE setup. Antioxidant
the wastelands as it can be grown on solic lands and stress response including Superoxide dismutase (SOD)
has drought resistance, yield potential, poverty alleviation enzyme assay, Total Ascorbate, Ascorbate peroxidase
and most importantly it will improve the income and (APX) enzyme assay, Flavanoid and Total Phenolic
livelihood of poor farmers. Also it will reduce India’s content (TPC) were determined in leaves sample in
import bill on oil often referred to as liquid gold. triplicates. Superoxide dismutase activity was found to
Biodiesel, a promising substitute as an alternative fuel decline in RGC 1002 [-16.63%] and RGC 1066 [-
has gained significant attention due to the predicted 17.90%] while total ascorbate, ascorbate peroxidise
shortness of conventional fuels and environmental activity, total phenol and flavanoid content increased in
concern. Jatropha is ecologically sustainable, eventually RGC 1002 [+9.37%, +6.30%, +11.53%, +10.46%] and
reduce the pollution burden. The utilization of liquid fuels RGC 1066 [+66.32%, +12.17%, 76.50%, +19.82%]
such as biodiesel produced from the non-edible under elevated carbon dioxide concentration in both
vegetable oil from Jatropha curcas by the cultivars. Micro nutrient availability and nutitional
transesterification process represents one of the most quality were estimated including Cr, Mn, Fe, Co, Cu,
promising options for the use of conventional fossil Zn, As, Se, Mo and Pb. Micro nutrient content declines
fuels. The Jatropha oil is converted into Jatropha oil under e[CO2] concentration in RGC 1002 including Fe
methyl ester known as biodiesel prepared in the [-56.00%], Cr[-62.14%], Co[-9.11%], Cu[-23.16%],
presence of homogeneous acid catalyst. The analysis Zn[-30.61%], Se[-13.57%], Mo[-38.42%], Pb[-
49.12%] excluding Mn[+32.29%] content. However, defences system. It was observed that the interaction
in RGC 1066 Cu[-52.81%], Zn[-20.29%], Se[- between e[CO2 ] and both plant varieties increased
19.00%], Mo[-31.54%], Pb[-40.21%] content declined uptake of micro-nutrients. Apart from these RGC 1066
where as Cr[+92.00%], Mn[+14.40%], Fe[+32.99%], varieties showed better uptake and translocation of
Co[+15.30%] content was found to increased under micro-nutrient efficiency (Cr, Mn, Co, Cu, Mo) than
e[CO2] concentration. Antioxidant response in both the RGC 1002 plant variety (Mo) under e[CO 2 ]
cultivars was enhanced under elevated carbon dioxide concentration. Thus, it can be concluded that RGC 1066
concentration that leads to the scavenging of ROS is better than RGC 1002 plant variety which is adapting
particles thus leading to declining of ROS and mitigating and performing in better way under e[CO 2 ]
the plant against abiotic stress condition. This conditions concentration.
leads to altogether improvement in plant antioxidant Keywords: e[CO2], a[CO2], FACE, ROS, SOD, APX.
Author Index
Author Index
D Dwivedi S. 160
Dabi H. 14 Dwivedi Sanjay 132, 139, 144, 155, 156, 157, 158, 167,
Das B. 14 185, 191, 205, 206, 232
Das Suchismita 111, 134 F
Das Suman 87, 88, 226 Faizan Rabia 92
Das Tapati 106 Fakhrah Shafquat 21, 236
Dasgupta Chitralekha Nag 92, 158 Falkenberg Gerald 132
Dasgupta Sabyasachi 120, 127 Farooq Fasuil 53
Deeba Farah 25 Farooqui Anjum 16, 228
Deka Sangeeta 21 Fassnacht Fabian Ewald 121
Dermann Anna 116 Fatima Touseef 26, 93
Dermann Florian 116 Fricke Annika 8
Devashree 229 Fujita Masayuki 181
Devkota Anjana 182 Funakawa Shinya 13
Dey Daniel C. 115, 127 G
Dey Mithra 219 Gangopadhyay Debnirmalya 88, 218
Dey Sumit Nath and Mithra 227 Gangwar Charu 221
Dhankher Om Parkash 131 Gao Yuan 148
Dhanya M.S. 86 Gardiner Emile S. 127
Dhyani S. 163, 224 Garg Arti 40
Dhyani S.D. 145 Garg Neera 81, 87, 91, 187, 177, 192, 194, 198, 200,
213
Dhyani Shalini 117
Gautam Ambedkar 168, 209
Dikshit Anupam 229
Geetanjali Monika 96
Dixit Garima 232
George V. 35
Dixit Sonal 147
Ghate Seemaa 138
Dixit Supriya 190
Ghosh Ashmita 88, 218, 226
Dixit Vijay Kant 94, 95, 100, 159, 212
Ghoshal Nandita 20, 110
Dubey Anamika 97
Gilhotra Vishakha 231
Dubey Arvind Kumar 24, 168, 172, 193
Giri Ved Prakash 93
Dubey N.K. 35
Godio A. 163
Dubey Priya 152
Gogoi Anudip 17
Dubey R.S. 202
Gogoi R.R. 123
Dubey Raghvendra 208
Gogoi Rashmi R. 124
Dubey Rama Kant 233
Gondal Amjad Shahzad 27
Dudhagi Shivanand Suresh 208
Goswami Sunayana 134
Dutta Venkatesh 31
Goyal Vinod 139, 191
Dwivedi Archana 207
Grantz David A. 195
Dwivedi Brajesh K. 141
Grill Erwin 7
Tamta S. 59 Tripathi Rudra Deo 81, 83, 84, 132, 139, 144, 150, 155,
Tan C. 217 156, 157, 160, 167, 183, 185, 191, 205, 206, 212
Tan M. 217 Tripathi R.S. 17, 118
Tandon Ashmita 91 Tripathi Sanjoli 163, 200
Taneja Lisha 165 Tripathi Shailja 64
Tarun 19 Tripathi Sunil 108
Taunk Rohit 228 Trivedi A.K. 22
Tayung Kumanand 225 Trivedi Anjali 228
Tewari Ashish 30 Trivedi Prabodh Kumar 83, 160, 183
Tewari D.D. 164 Tuli R. 19
Tewari Lalit Mohan 202 Tyagi Nidhi 170
Tewari Rajesh K. 142 U
Tewari Sri Krishna 112, 155 Uadav Udit 91
Thadani Rajesh 122 Uddin Moin 190
Thakkar Vidhatri 228 Umar Shahid 188, 225
Thakur Monika 89 Uniyal Sanjay Kumar 42, 49
Thapa Namita 123 Upadhaya K. 123, 124
Thomas Binoy T. 41, 50 Upadhaya Krishna 126
Thomas V.P. 41, 50 Upadhyay A.K. 213
Tiwari Avinash 67 Upadhyay Atul Kumar 143
Tiwari Gopal Ji 122, 126 Upadhyay Munish Kumar 142, 186, 197
Tiwari J.K. 237 Uppadhyay Vindhyeshwari 166
Tiwari K.K. 162, 173 Upreti D.K. 56, 61, 166
Tiwari O.N. 75 Upreti Priyanka 202
Tiwari Poonam 100 V
Tiwary Raghuvar 119 Vajpayee Poornima 99, 183, 207
Todaria N.P. 120, 127 Varshney C.K. 3
Tomar Anita 49 Varshney Garima 221
Toppo Kiran 60, 68, 92, 158 Verma Ashok K. 202
Toyama Hironori 119 Verma Isha 26, 93
Tripathi A.K. 236 Verma Lata 137
Tripathi A.N. 236, 238 Verma Laxmi 186
Tripathi Anamika 221 Verma P. 160
Tripathi Arpita 99 Verma Pratibha 31, 94, 204
Tripathi Ashutosh 93 Verma Praveen C. 202
Tripathi Om Prakash 14, 21, 17 Verma Preeti 24
Tripathi Pratibha 81 Verma R.K. 59
Tripathi Preeti 84 Verma S.K. 150
Vice-Presidents
Secretary
Dr. K.J. Ahmad (Lucknow)
Additional Secretary
Dr. R.D. Tripathi (Lucknow)
Joint Secretaries
Treasurer
Dr. D.K. Upreti (Lucknow)
Councilors
Advisors
Prof. J.N.B. Bell (U.K.) Dr. B.P. Singh (Lucknow)
Prof. C.R. Bhatia (Mumbai) Prof. R.S. Tripathi (Lucknow)
Prof. C.K. Varshney (New Delhi)
Prof. R.K. Kohli (Bathinda)
Prof. H.N. Verma (Jaipur)
Dr. P.V. Sane (Lucknow)
Prof. P.K. Seth (Lucknow)