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METHODS
The TAC of Sixteen multibacillary leprosy patients which fullfill the inclusion criteria
were assessed before and three months after receiving WHO MDT-MB therapy. The numbers
were then evaluated.
Measure levels of TAC plasma :
• Sample : Collect blood 5cc from vena cubiti using spoit and add to EDTA or heparin as an
anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30
minutes of collection. Samples should be aliquoted or must be stored at -80°C for 6 months
using.
• Reagent Provided : Standard Diluent, Streptavidin-HRP, Stop Solution, Substrate Solution
A , Substrate Solution B , Wash Buffer Concentrate (30x), Biotin-Conjugate Anti-human
T-AOC Antibody.
• Assay Principle : This kit is a Enzyme-Linked Immunosorbent Assay (ELISA). T-AOC is
added to the wells pre-coated with T-AOC monoclonal antibody. After cubation a biotin-
conjugated anti-human T-AOC antibody is added and binds to human T-AOC. After
incubation unbound biotin-conjugated anti-human T-AOC antibody is washed away during
a washing step. Streptavidin-HRP is added and binds to the biotin-conjugated anti-human
T-AOC antibody. After incubation unbound Streptavidin-HRP is washed away during a
washing step. Substrate solution is then added and color develops in proportion to the
amount of human T-AOC. The reaction is terminated by addition of acidic stop solution and
absorbance is measured at 450 nm.
CONCLUSION
EXPRESSION OF GRATITUDE