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Macr SEVERN AND BREWING MATERIALS (Chapter 27, p. 4 tract directly from 970.90 (see Appendix C). If 100 mL beer was correct as follows Extract found x specific gravity of dealeoholized beer/specific gravity of beer = gextract/100 g beer Revised: March 1996 271.09 AOAC Official Method 935.20 Extract of Original Wort from Beer First Action 1935 Final Action Caleulate from following formula and report to frst decim place: = ((P 2.0665) + £) x 100 100% (P 10665) where 0 =extvact of original wort;P=% alcohol by weight (g/100 beer): and E = % real extract, 925.091B(a) ot (b) (see 321.02 27.1.10 AOAC Official Method 935.21 ‘Alcohol (by Volume) in Specific Gravity Method First Action 193 Final Action See 920.57 (see 28.1.08), but use pycnameters deséribed ir 935.30B4i) and 935-30C(b) or (e) (sce 27.3.07). 27a AOAC Official Method 935.22 ‘Alcohol (by Weight) in Beor Specific Gravity Method First Act'on 1935 Final Action yee 945.07 (see 26,1.12), But use pyenometers des 935.3084) and 935.30C(b) or () (see 27.3.07) 27.41.12 AOAC Official Method 970.15 Alcohol (by Weight) in Beer Refractometer Method First Action 1970 Final Action 1871 A. Apparatus (a) Immersion refractometer—Carl Zeiss, Germany, PO Box 1369/1380, D-7082, Oberkochen, Germany, or equivalent, with ge 132-137 n prisms covering r (b) Water bath—See 935.30B(k) (see 27.3.07) (©) Prenometer—see 9 213.01), BLA) and 935.30C() oF (©) (see ‘©2000 AORG INTERNATIONAL ‘AOAC Orneit Menscos.or Ava.yais (2000) B. Callbration ‘Adjust refractometer light to give maximum contrast between light and dark fields. Adjust color compensator and focus for sbarp, color-free dividing line. H,O, double-disilled from glass, should read €a 14.50 at 20°C. Read H,0 before each sample series. Rinse ‘prism with H,O after each sample and dry with soft issue. Prepare calibration curve to convert refractometer readings and specific gravity determinations to % alcohol by weight by analyzing Doers covering alcohol range of interest by 935.22 (see27.1.11) and Plot results, using abscissa (RN), where reading (Rize~ Rese)» N= 1000 (specific gravity — 1.00000), and ordinate = % alcohol by weight, 935.22 (see 27.1.11). Fit least squares line to adequate number of points to get accuracy desire. ‘Determine equation of ine and slope. (See Definitions of Terms and [Explanatory Notes.) Calculate % alcohol by weight by formula or read from calibration curve, alcohol by weight = F (RN) + C, lope of calibration line, and C= constant of calibration curve equation = refiactometer ©. Determination Determine specific gravity of decarbonated beer by 920.80 (see 1.05); Place refraciomete> cuvet containing distilled 1,0 and clear decarbonated beer samples in 20°C H,0 bath, Place prism of refractometer in H,O cuvel and check temperature after 15 mi. Make 5 readings to nearest 0.1 scale division and average results (®ene)“Fansfer dry prism to bees samp!s cx 2t, wait 21 min, make 5 readings to nearest 0.1 scale di. ision, and average results (Rs) Calculate % aleohol by weight by formula or read from calibration Reference: ASBC: Beer $C. CAS-64-17-5 (alcohol) prs AOAC Oficial ttothod 984.14 Ethanol in Beor “Gas chromitogrphic ethos! Fiat Acton 1804 Firat Aton 1988 ASBC-AOAC Method A. Principle n-Propanol internal standard is added to sample, and ethanol is de {termined by GC using flame ionization detection. B. Reagents and Apparatus (a) m-Propanal—Internal standard 5% aqueous stock solution. Refrigerate (b) Ethanol standard solutions —3, 4, 5, 6, 7, and 8% aqueous ethanol solutions. Determine exact % ethanol by pycnometer, 942,06B(a) (see 26.1.07), hydrometer, 957.034. (see 26,1.08), oF refractometer, 950.04 (see 26.1.10). Altematively, prepare standard solutions by quantitative dilution of concentrated ethanol solution hniques. Keep solutions refrigerated. analyzed by one of above (©) Gas chromatograph—With flame ionization detector and {it Jin. (1.8 mx 0.3m) stainless steel or glass column containing 80-100 mesh Chromosorb 103. He of Ny carrier gas 20 mL/min; in Jector temperature 175°C; column temperature 185°C isothermal (adjust temperature so ethanol elutes in ¥ min, n-propanol in 1.6 ‘AOAC Orne, MeTH905 oF Aussi (2000) Macr Bevenaces ano Breve MarEnaLs 1 Chapter 27, p.5 ‘min; detector ternperature 250°C; chart speed and atenuation ase uired based on instrument used ©. Calibration Pipet 5.0 mL ethanol standard solutions into separate sglass-stoppered flasks, Add 5.0 mL intemal standard solution (0 each and snix weil Inject 0.2 yl of each solution in duplicate and ‘measure peak heights (integrator may be used). Calculate ratio of ethanol to n-propanol peaks and average foreach concentration. Plot ratio against concentration and ealeulate slope of line (F). Repeat analysis of 5% ethanol standard solution each day. . Determination ecarbonate beer by filteting through S&S 560 or equivalent pa- per. Pipet 5.0 ml into glass-stoppered flask. Add S.0 mL, aqueous ‘n-propanol intemal sterdaed solution, Mix thoroughly by switling, and inject 0.2 pL onto GC column, ad determine ratio of ethanol to rn-propanol peaks. & Cateulation Ethanol, % (wh) = tnanol / peak height propanol F peak height Reference: JAOAC 67, 192(1984). AOAC Official Method 992.29 Ethanol and Original Gravity Content in Beor SCABA Mathod First Action 1892 ASBC-AOAC Method (Applicable to determination of exiginal gravity, 3-15, and etha- nol, 1-6% {w/w}, in beer) Results ofthe interlaboratory study supporting the acceptance ofthe method, Ethanol, % (w/w) (006; sp = 0.036; RSD, = 0.156%; RSDp Original gravity, O18: 5, A. Principle ‘The instrument calculates original gravity, *Plato, from measure- ‘ment of density with density meter and measures ethanol, % (wx), by catalytic combustion of ethanol vapor, from evaporated boer sample, in ai steam passing over sensor surface. 1B. Reagents and Apparatus (a) Automatic beer analyzer-—Capable of calculating original ‘gravity from measuring density ofbeer (Anton Paar density meters suitable). Capable of calculating % (ww) ethanol inbeer; equipped ‘with peristaltic pump (to pass samples through analyzer), therm0- statically controlled eal (o evaporate ethanol in sample), tubing (to ‘mix seam of air with ethanol vapor), and oven with ettanol sensor (focombust and measure ethanol). Microcomputer control uniteval- uates and prints outs results. (Tecator SCABA Automatic Beer Ana lyzer, PO Box 110, $-162 12, Stockholm, Sweden, is suitable. 939% (091; RSD, # 0.168%; RSD, = 0.828% (b) Ethanol standard solutions.—Propare 3.5 and 7.0% (wi) cethanol sandr solutions by diluting 46.4 ml. and 92.3 mLofetha- ‘no (99.58 minimum parity) to velurme with H,O in separate 1 L volumettc Nass. Discard after 2 weeks, or system suitablity tet, determine % ethanol to nearest 0.01% by pycnometer, 942.06 (see 26.1.07), or refractometer, 950.04 (see 261.10). (©) Conditioning solution. Dissolve ea 7 g chlorine-feee deter- {gent (Tecator Cat. No. 5000-1928 is suitable) inc 500 mL 1,0 in 1L volumetric ask. Add'75 ml.ethano!, and dilute to volume with @ Instrument Conditioning and Calforation ‘Turn on analyzer and control unit. Select microcomputer program for desired analysis, adjust ait ow to analyzer requirements, and ‘connect tubing for peristaltic purmp. ‘Tocondition analyzer, place almost full vil of conditioning solu tion one syace hina sampling position. When oven and thermostat indicate run temperatures have been reached, start pump and regirculate conditioning solution for 20 min by placing end of drain age tube in vik Calibrate analyzer by placing vial with HO one space behind sampling position, directly followed by vial with3.9%ethen vial with 7.0% ethanol standard solutions, B(b). Select calibration program. and process vials, . Determination Degas beer by 920.49 (see 27.1.01). Turbid samples must be fil- tered, Fill somple vils atleast full and load instrument turmable. Select desized analysis programs and process vials. Read calculated values for original gravity and eihanol (Ss {w/v} and [w/) from contol unit printout ‘To maintain instrument in standby mode (>10 min between measurements), place almost full viel of conditioning solution behind sample vials, leaving one empty space between them, When instrument begins to pump conditioning solution, recirculate conditioning solution by placing end of drainage tube in conditioning solution vial. Replace vial with fresh condition- ing solution every 1-2 h. ‘At completion of analysis, pump 1 vial of conditioning solution hough instrument then 1 vial of HO. When all H,0 has been ‘pumped out, tum off pump, control unit, and then analyzer. Unhook ppamp wbes, Reference: J. AOAC In. 76, 918(1993), ASDC: Beer-4, Revised: March 1997 274.18 AOAC Official Method 950.06 Fermentation of Beer First Action 1950 Final Action A. Real Degree of Fermentation or Real Atteauation Calculate as follows and report to first decimal plave: (Original extract ~ rel extract) x (100Voriginal extract) x (UA ~ [0.005161 x real extract) Reference: ASBC: Beor-6,E, ‘WROD AOAC INTERNAL