Department of Laboratory Services

Ministry of Health
Brunei Darussalam

Venipuncture Procedure

Step 1 Step 2 Step 3

Label the tube with the patient’s Put tourniquet on the patient about Ask patient to form a fist so
particulars 3-4’ above the venipuncture site veins are more prominent

Step 4 Step 5 Step 6

After finding the vein, clean the Assemble needle and vacuum Insert the collection tube into the
venipuncture site with alcohol using tube holder holder until the tube reaches the
circular motion. Allow the area to dry needle

Step 7 Step 8 Step 9

Remove cap from needle Use thumb to draw skin tight Hold the skin tight through step 10.
about 1-2” below the Insert the needle, bevel side up,
venipuncture site into the vein

Step 10 Step 11 Step 12

Push the tube completely onto the Release the tourniquet After opening the patient’s hand, place
needle. Blood should begin to flow dry gauge over the venipuncture site
into the tube until vacuum tourniquet is and slowly remove the needle
exhausted

Step 13 Step 14 Step 15

Apply mild pressure to the pad Apply bandage or continue applying mild Properly dispose of all contaminates
pressure until bleeding has stopped supplies in sharp / biohazard container

Step 16
Place request form and specimen in biohazard bag

Extracted from: LI-PHL-1 (PHLEBOTOMY PROCEDURE), Pg. 6, Edition 3, Issue date: 12 July 2012

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