VALIDASI 1 (Aris dkk)

A. BAKU

Konsentrasi (ppm) AUC

50 221.9
100 1149.8
200 2405.3
300 1975.5
400 3585.2
500 2583.2

A: 549.946

B: 5.562

R:0.825

Y= 5.562x + 549.946

B. RF ADISI

RF adisi konsentrasi... Replikasi Rf Rata rata

(ppm)
50 1 0.97
2 0.97
3 0.97
200 1 0.98
2 0.98 0.97
3 0.98
500 1 0.96
2 0.96
3 0.96

C. KADAR BAKU ADISI

a. 50 ppm
R1 AUC 846.4
Kadar: 846.4 = 5.562x + 549.946
X = 53.29 ppm
R2 AUC 1327.9 Analisis statistik
Kadar: 1327.9 = 5.562x + 549.946 rerata : 106.358
X = 139.869 ppm SD : 46.485
CV : 43.706%

R3 AUC 1250.3 %recovery

106.358 𝑝𝑝𝑚
Kadar: 1250.3 = 5.562x + 549.946 x 100% = 212.716 %
50 𝑝𝑝𝑚
X = 125.917 ppm

b. 200 ppm
R1 AUC 2057.8
Kadar: 2057.8 = 5.562x + 549.946
X = 270. 956 ppm

R2 AUC 2506.9 Analisis statistik

Kadar: 2506.9 = 5.562x + 549.946 rerata : 323.646 ppm
X = 351.681 ppm SD : 45.662
CV : 14.1 %

R3 AUC 2487.2 %recovery

323.646 𝑝𝑝𝑚
Kadar: 2487.2 = 5.562x + 549.946 x 100% = 161. 823%
200 𝑝𝑝𝑚
X = 348.301 ppm

c. 500 ppm
R1 AUC 6410.4
Kadar: 6410.4 = 5.562x + 549.946
X = 1053.659 ppm

R2 AUC 4344 Analisis statistik

Kadar: 4344 = 5.562x + 549.946 rerata : 803. 587 ppm
X = 682. 138 ppm SD : 216.598
CV : 26.953 %
R3 AUC 4304.1 %recovery
803.587 𝑝𝑝𝑚
Kadar: 4304.1 = 5.562x + 549.946 x 100% = 160. 717%
500 𝑝𝑝𝑚
X = 674. 964 ppm
 VALIDASI 2 (Epen dkk)

A. BAKU

Konsentrasi (ppm) AUC

50 776.2
100 278.3
200 2288.3
300 1331.9
400 -
500 -

A: 1261.12

B: -1.865

R: -0.361

Y= -1.865x + 1251.12

B. AUC ADISI

RF adisi konsentrasi... Replikasi AUC

(ppm)
100 1 2826.8
2 3436
3 8386.7
300 1 9690.3
2 7825.7
3 11820.4
500 1 10039.2
2 8570
3 2111.7

C. KADAR BAKU ADISI

a. 100 ppm
R1 AUC 2826.8
Kadar: 2826.8= -1.865x + 1251.12
X = -889. 506 ppm
R2 AUC 3436 Analisis statistik
Kadar: 2826.8= -1.865x + 1251.12 rerata : -1958.77
X = -1166.15 ppm SD : 1618.37
CV : 82.62%

R3 AUC 8386.7 %recovery

−1958.77 𝑝𝑝𝑚
Kadar: 8386.7 = -1.865x + 1251.12 x 100% = 1958.77 %
100 𝑝𝑝𝑚
X = -3820.68 ppm

b. 300 ppm
R1 AUC 9690.3
Kadar: 9690.3 = -1.865x + 1251.12
X = -4519.66 ppm

R2 AUC 7825.7 Analisis statistik

Kadar: 7825.7 = -1.865x + 1251.12 rerata : -4567. 11 ppm
X = -3519.88 ppm SD : 1071.75
CV : 23.46 %

R3 AUC 11820. 4 %recovery

−4567.11 𝑝𝑝𝑚
Kadar: 11820. 4 = -1.865x + 1251.12 x 100% = 1522.37%
300 𝑝𝑝𝑚
X = -5661.81 ppm

c. 500 ppm
R1 AUC 10039.2
Kadar: 10039.2= -1.865x + 1251.12
X = -4730.87 ppm

R2 AUC 8570 Analisis statistik

Kadar: 8570 = -1.865x + 1251.12 rerata : -3043.34 ppm
X = -3918.97 ppm SD : 1842.47
CV : 60.54 %
R3 AUC 2111.7 %recovery
−3043.34 𝑝𝑝𝑚
Kadar: 2111.7 = -1.865x + 1251.12 x 100% = 608.66%
500 𝑝𝑝𝑚
X = -480.2 ppm
 PENETAPAN KADAR 1 (Ria Nonita dkk)

A. KURVA BAKU

Konsentrasi (ppm) AUC

50 3676.0
100 5908.7
200 3955.9
300 3484.7
400 6887.8
50 11403.9

A : 2582.7

B : 12.78

R : 0.736

Y= 12.78x + 2582.7

 Digunakan persamaan dari validasi 1 (y=5.562x + 549.946)

B. AUC SAMPEL

Sampel AUC Kadar (ppm)

1 5131.9 823.79
2 3568.3 542.67
3 3339.6 501.56
4 2036.4 267.25
5 11579.8 1983.07
6 7586.1 1265.04
7 3646.2 556.68
8 7673.6 1280.77

Rerata : 902.604 ppm

SD : 568.42

CV : 62.98 %

%Recovery

902.604 mikrogram/mL x 50 mL = 45.130 mg

45.130 𝑚𝑔
x 100 % = 361.04 %
12.5 𝑚𝑔